Tissue fluid extraction equipment

Abstract

The invention discloses tissue fluid extraction equipment, and relates to the technical field of health monitoring. The tissue fluid extraction equipment comprises an alternating current source and an electrode, the output end of the alternating current source is connected with the electrode, the alternating current source is used for outputting set alternating current signals of preset times to the electrode according to a preset time interval, the preset time interval is larger than 0, and the preset times are larger than or equal to 2; the electrode is used for applying electrical stimulation corresponding to an alternating current signal output by the alternating current source to the tissue fluid extraction position, and the tissue fluid extraction position releases tissue fluid under the electrical stimulation. The tissue fluid extraction equipment can realize non-invasive tissue fluid extraction.

Description

Technical Field

[0001] The present application relates to the field of health monitoring technology, and more specifically, to a tissue fluid extraction device. Background Art

[0002] Human tissue fluid is a liquid that maintains a stable environment for human tissue cells. It contains many biomarkers closely related to human health indicators, such as glucose, electrolytes, and lactate. Therefore, extracting human tissue fluid and then testing for biomarkers in it can detect human health indicators.

[0003] Current interstitial fluid extraction devices primarily extract interstitial fluid through invasive methods. However, these devices require microneedles to penetrate the skin, which can cause trauma and infection. Therefore, achieving non-invasive interstitial fluid extraction has become a pressing technical challenge. Summary of the Invention

[0004] One object of the present application is to provide a new technical solution for tissue fluid extraction.

[0005] According to a first aspect of the present application, a tissue fluid extraction device is provided, comprising: an alternating current source and electrodes, wherein: The output end of the alternating current source is connected to the electrode, and is configured to output a preset alternating current signal to the electrode for a preset number of times according to a preset time interval, wherein the preset time interval is greater than 0, and the preset number of times is greater than or equal to 2; The electrodes are used to apply electrical stimulation corresponding to the AC current signal output by the AC current source to the tissue fluid extraction site, and the tissue fluid extraction site releases tissue fluid under the electrical stimulation.

[0006] Optionally, the tissue fluid extraction device further includes: a detection element and a processor, wherein: The detection member is used to detect whether the electrode is attached to the tissue fluid extraction site; The processor is respectively connected to the output end of the detection component and the control end of the AC current source, and is used to control the AC current source to be in a working state of outputting a set AC current signal to the electrode for a preset number of times according to a preset time interval when the detection component determines that the electrode is applied to the tissue fluid extraction site.

[0007] Optionally, the tissue fluid extraction device further comprises: a conductive liquid dripping device, wherein: The control end of the conductive liquid dripping component is connected to the processor, and the processor is also used to control the conductive liquid dripping component to drip conductive liquid to the tissue fluid extraction site before controlling the AC current source to be in a working state of outputting a set AC current signal to the electrode for a preset number of times according to a preset time interval when it is determined by the detection component that the electrode is applied to the tissue fluid extraction site.

[0008] Optionally, the tissue fluid extraction device further comprises: a cleaning liquid dripping device, wherein: The control end of the cleaning liquid dripping component is connected to the processor, and the processor is also used to control the cleaning liquid dripping component to drip cleaning liquid to the tissue fluid extraction site before controlling the conductive liquid dripping component to drip conductive liquid to the tissue fluid extraction site when it is determined by the detection component that the electrode is applied to the tissue fluid extraction site.

[0009] Optionally, the reference current amplitude of the AC current signal has a value range of [100, 250] μA, the variation amplitude has a value range of [10, 50] μA, and the frequency has a value range of [5, 200] Hz.

[0010] Optionally, the reference current amplitude of the AC current signal is 150 μA, the variation amplitude is 20 μA, and the frequency is 20 Hz.

[0011] Optionally, the preset time interval has a value range of (0, 30] s.

[0012] Optionally, the preset time interval is 10s.

[0013] Optionally, the preset number of times is [5, 20] times.

[0014] Optionally, the preset number of times is 10 times.

[0015] The present application provides a tissue fluid extraction device comprising: an alternating current source and electrodes, wherein the output end of the alternating current source is connected to the electrodes and is configured to output a set alternating current signal to the electrodes at a preset time interval for a preset number of times, wherein the preset time interval is greater than 0 and the preset number of times is greater than or equal to 2; the electrodes are configured to apply electrical stimulation corresponding to the alternating current signal output by the alternating current source to a tissue fluid extraction site, causing the tissue fluid extraction site to release tissue fluid under the electrical stimulation. This tissue fluid extraction device can achieve non-invasive tissue fluid extraction. Furthermore, on the one hand, the tissue fluid extraction device can apply stable electrical stimulation to the tissue fluid extraction site during tissue fluid extraction. On the other hand, this tissue fluid extraction device can also reduce pain and skin redness and swelling at the tissue fluid extraction site during tissue fluid extraction, while also increasing the tissue fluid extraction rate. Furthermore, the electrical stimulation is intermittent, which can induce a contraction reaction at the tissue fluid extraction site, further increasing the tissue fluid extraction rate.

[0016] Other features and advantages of the present application will become apparent from the following detailed description of exemplary embodiments of the present application with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate embodiments of the application and, together with the description, serve to explain the principles of the application.

[0018] Figure 1 This is a schematic structural diagram of a tissue fluid extraction device provided by the present application; Figure 2 It is a structural schematic diagram of another tissue fluid extraction device provided by the present application; Figure 3 This is a waveform diagram of a set AC current signal provided by this application; Figure 4 This is a schematic diagram of the sodium ion concentration of the extracted tissue fluid after dilution to the same degree under different types and preset times of current signals provided in an embodiment of the present application; Figure 5 1 is a schematic diagram of sodium ion concentrations of tissue fluid extracted by a tissue fluid extraction device after dilution to the same degree under AC current signals of different reference current amplitudes output by an AC current source, provided in an embodiment of the present application; Figure 6 This is a schematic diagram of the sodium ion concentration of the extracted tissue fluid after dilution to the same degree under the AC current signals of different changing amplitudes output by the AC current source, provided in an embodiment of the present application. DETAILED DESCRIPTION

[0019] Various exemplary embodiments of the present application will now be described in detail with reference to the accompanying drawings. It should be noted that unless otherwise specifically stated, the relative arrangements of components and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present application.

[0020] The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the present disclosure, its application, or uses.

[0021] Technologies, methods, and equipment known to ordinary technicians in the relevant art may not be discussed in detail, but where appropriate, the technologies, methods, and equipment should be considered part of the specification.

[0022] In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not limiting. Therefore, other examples of the exemplary embodiments may have different values.

[0023] It should be noted that like reference numerals and letters refer to like items in the following figures, and therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0024] The present application provides a tissue fluid extraction device 100, such as Figure 1 As shown, it includes an AC current source 110 and an electrode 120, wherein: The output end of the AC current source 110 is connected to the electrode 120 and is configured to output a preset number of set AC current signals to the electrode 120 at a preset time interval, wherein the preset time interval is greater than 0 and the preset number of times is greater than or equal to 2; The electrode 120 is used to apply electrical stimulation corresponding to the AC current signal output by the AC current source 110 to the tissue fluid extraction site, and the tissue fluid extraction site releases tissue fluid under the electrical stimulation.

[0025] In this embodiment, the interstitial fluid extraction device 100 includes an AC current source 110 and an electrode 120. In one embodiment of the present application, the AC current source 110 is provided with a start button. When the start button is activated, the AC current source 110 outputs a set AC current signal to the electrode 120 a preset number of times at a preset time interval. In one example, the AC current source 110 is a sinusoidal AC current source that outputs a sinusoidal AC signal.

[0026] As well as Figure 1As shown, the electrode 120 generally includes a working electrode and a counter electrode, and the working electrode and the counter electrode are respectively connected to the AC current source 110 to form an electrical circuit. In one embodiment of the present application, the specific preparation method of the electrode 120 is: a mixed conductive paste is printed on the surface of the substrate by screen printing, and the area of ​​the printed two electrodes working area is 0.2-0.8 ( The mixed conductive paste is preferably a mixture of carbon black, graphene paste, carbon nanotubes, nano-gold, and silver paste. In one embodiment, the mixed conductive paste is a mixture of carbon black and nano-gold paste, and the substrate material is polyvinyl alcohol, polyimide, and cellulose paper.

[0027] It should be noted that if Figure 3 As shown, the above-mentioned preset time interval is greater than 0, that is, the AC current source 110 may stop outputting the AC current signal to the electrode 120. Based on this, the electrode 120 may stop applying the AC current signal to the tissue fluid extraction site. In this case, the electrical stimulation received by the tissue fluid extraction site is intermittent. In addition, the above-mentioned preset number of times is greater than or equal to 2, that is, the AC current source 110 outputs the AC current signal to the electrode 120 in a cycle of at least 2 times. Based on this, the electrode 120 applies the electrical stimulation corresponding to the AC current signal to the tissue fluid extraction site in a cycle of at least 2 times.

[0028] In conjunction with the above, when the tissue fluid extraction device is used by a user, the electrode 120 is applied to the tissue fluid extraction site, such as the fingertip. The AC current source 110 outputs a set AC current signal to the electrode 120 for a preset number of times at a preset time interval. Electrode 120 then applies electrical stimulation corresponding to the set AC current signal output by the AC current source 110 for a preset number of times at a preset time interval to the tissue fluid extraction site. Consequently, the tissue fluid extraction site receives electrical stimulation corresponding to the AC current signal output by the AC current source 110 (the set AC current signal output for a preset number of times at a preset time interval to the electrode). This electrical stimulation creates an electric field at the tissue fluid extraction site. Under the action of the electric field, the tissue fluid undergoes ion migration, cell membrane electroporation, and electroosmosis, allowing the tissue fluid to permeate through the skin. In this way, non-invasive tissue fluid extraction can be achieved.

[0029] Furthermore, in this embodiment, the AC current source 110 applies electrical stimulation corresponding to an AC current signal to the tissue fluid extraction site via the electrodes 120. On the one hand, compared to electrical stimulation corresponding to a voltage signal, this electrical stimulation does not change due to changes in the state of the extraction object (e.g., the human body), thereby achieving stable electrical stimulation corresponding to the AC current signal applied to the tissue fluid extraction site via the electrodes. Furthermore, the electrical stimulation is an AC current signal, and the direction of the current corresponding to the AC current signal periodically alternates, resulting in relatively dispersed stimulation of the nerves and tissues at the tissue fluid extraction site. This also prevents large molecular proteins in the tissue fluid from blocking the skin tissue channels, thereby increasing the permeability of the tissue fluid. This reduces skin irritation at the tissue fluid extraction site and increases the tissue fluid extraction rate. In other words, the tissue fluid extraction device provided in this embodiment can reduce pain and skin redness and swelling at the tissue fluid extraction site during tissue fluid extraction, while also increasing the tissue fluid extraction rate. Furthermore, the electrical stimulation is intermittent, which can induce a contraction reaction at the tissue fluid extraction site, further increasing the tissue fluid extraction rate.

[0030] The present application provides a tissue fluid extraction device comprising: an alternating current source and electrodes, wherein the output end of the alternating current source is connected to the electrodes and is configured to output a set alternating current signal to the electrodes at a preset time interval for a preset number of times, wherein the preset time interval is greater than 0 and the preset number of times is greater than or equal to 2; the electrodes are configured to apply electrical stimulation corresponding to the alternating current signal output by the alternating current source to a tissue fluid extraction site, causing the tissue fluid extraction site to release tissue fluid under the electrical stimulation. This tissue fluid extraction device can achieve non-invasive tissue fluid extraction. Furthermore, on the one hand, the tissue fluid extraction device can apply stable electrical stimulation to the tissue fluid extraction site during tissue fluid extraction. On the other hand, this tissue fluid extraction device can also reduce pain and skin redness and swelling at the tissue fluid extraction site during tissue fluid extraction, while also increasing the tissue fluid extraction rate. Furthermore, the electrical stimulation is intermittent, which can induce a contraction reaction at the tissue fluid extraction site, further increasing the tissue fluid extraction rate.

[0031] In one embodiment of the present application, Figure 2 As shown, the tissue fluid extraction device 100 provided in this application further includes: a detection element 140 and a processor 130, wherein: The detection member 140 is used to detect whether the electrode 120 is attached to the tissue fluid extraction site; The processor 130 is respectively connected to the output end of the detection component 140 and the control end of the AC current source 110, and is used to control the AC current source 110 to be in a working state of outputting a set AC current signal to the electrode for a preset number of times according to a preset time interval when the detection component 140 determines that the electrode 120 is applied to the tissue fluid extraction site.

[0032] In this embodiment, detection element 140 detects whether electrode 120 is attached to the tissue fluid extraction site and further reports the detection result to processor 130. If processor 130 determines that electrode 120 is attached to the tissue fluid extraction site based on the detection result reported by detection element 140, it controls AC current source 110 to output a set AC current signal to electrode 120 a preset number of times at preset time intervals. Based on this, unnecessary output of AC current signals by AC current source 110 can be avoided, thereby reducing the power consumption of the tissue fluid extraction device 100 provided herein.

[0033] The detection member 140 can be exemplarily at least one of a temperature sensor, a pressure sensor, and an infrared sensor. The detection member 140 is typically disposed on the surface of the electrode 120 for application to the tissue fluid extraction site. If the detection member 140 is a temperature sensor, if the temperature detected by the detection member 140 is the temperature of the extraction object, it is determined that the electrode 120 is applied to the tissue fluid extraction site. If the detection member 140 is a pressure sensor, if the detection member 140 detects a pressure signal, it is determined that the electrode 120 is applied to the tissue fluid extraction site. If the detection member 140 is an infrared sensor, if the detection member detects an infrared signal, it is determined that the electrode 120 is applied to the tissue fluid extraction site.

[0034] Furthermore, the processor 130 may be exemplarily an MCU.

[0035] In one embodiment of the present application, Figure 2 As shown, the tissue fluid extraction device 100 provided in the present application further includes: a conductive liquid dripping component 150, wherein: The control end of the conductive liquid dripping component 150 is connected to the processor 130. The processor 130 is also used to control the conductive liquid dripping component 150 to drip conductive liquid to the tissue fluid extraction site before controlling the AC current source 110 to be in a working state of outputting a set AC current signal to the electrode 120 for a preset number of times according to a preset time interval, when it is determined through the detection component 140 that the electrode 120 is applied to the tissue fluid extraction site.

[0036] In this embodiment, the conductive liquid dripping device 150 is used to drip conductive liquid into the tissue fluid extraction site. The conductive liquid is used to increase the conductivity between the electrode 120 and the tissue fluid extraction site. In one embodiment of the present application, the conductive liquid is phosphate buffered saline (PBS). Furthermore, 50 μL of conductive liquid is dripped into the conductive liquid dripping device at a time.

[0037] Furthermore, when the processor 130 determines that the electrode 120 is attached to the tissue fluid extraction site, it first controls the conductive liquid dripping element 150 to drip conductive liquid to the tissue fluid extraction site before controlling the AC current source 110 to output a set AC current signal to the electrode 120 a preset number of times at a preset time interval. This prevents the problem of the tissue fluid extraction site drying out, which could lead to a decrease in the intensity of the electrical stimulation corresponding to the AC current signal output by the AC current source 110 applied by the electrode 120 to the tissue fluid extraction site, and thus a decrease in the rate of tissue fluid release from the tissue fluid extraction site.

[0038] In one embodiment of the present application, Figure 2 As shown, the tissue fluid extraction device 100 provided in the present application further includes: a cleaning liquid dripping component 160, wherein: The control end of the cleaning liquid dripping component 160 is connected to the processor 130. The processor 130 is also used to control the cleaning liquid dripping component 160 to drip cleaning liquid to the tissue fluid extraction site before controlling the conductive liquid dripping component 150 to drip conductive liquid to the tissue fluid extraction site when it is determined through the detection component 140 that the electrode 120 is applied to the tissue fluid extraction site.

[0039] In this embodiment, the cleaning liquid dripping member 160 is used to drip cleaning liquid to the tissue fluid extraction site. The cleaning liquid is used to clean the tissue fluid extraction site. In one embodiment of the present application, the cleaning liquid is alcohol or iodine tincture.

[0040] Furthermore, when the processor 130 determines through the detection element 140 that the electrode 120 is attached to the tissue fluid extraction site, it first controls the cleaning fluid dripping element 160 to drip a cleaning fluid into the tissue fluid extraction site before controlling the conductive fluid dripping element 150 to drip a conductive fluid into the tissue fluid extraction site. This prevents the tissue fluid released from the tissue fluid extraction site from being contaminated by contaminants.

[0041] In one embodiment of the present application, the base current amplitude of the AC current signal is in the range of [100, 250] μA, the variable amplitude is in the range of [10, 50] μA, and the frequency is in the range of [5, 200] Hz. When using an AC current signal within these ranges to extract tissue fluid, the amount and rate of tissue fluid extracted can be balanced and reasonable. The variable amplitude refers to the difference between the peak and valley values ​​of the AC current signal.

[0042] In one embodiment of the present application, the reference current amplitude of the AC current signal is 150 μA, the variation amplitude is 20 μA, and the frequency is 20 Hz.

[0043] In one embodiment of the present application, the preset time interval is in the range of (0, 30] s. On this basis, rapid extraction of tissue fluid can be achieved while taking into account skin irritation of the extraction object. In one embodiment of the present application, the preset time interval is 10 s.

[0044] In one embodiment of the present application, the preset number of times is in the range of [5, 20]. On this basis, rapid extraction of tissue fluid can be achieved while taking into account skin irritation of the subject. In one embodiment of the present application, the preset number of times is 10.

[0045] Based on the above embodiment, the waveform of the set AC current signal output by the AC current source in the tissue fluid extraction device provided by the present application is as follows: Figure 3 shown.

[0046] Example 1: Step 1: Print the carbon black and nano-gold mixed conductive paste onto the substrate surface by screen printing. The working area of ​​the two printed electrodes is , then put it into an oven and dry it at 120℃ for 1h; Step 2, AC current source design: output a preset time interval of 10s, set the base current amplitude of the AC signal to 150μA, the variation amplitude to 10μA, the frequency to 20Hz, and set the duration of the AC signal to a sinusoidal AC signal of 30s. Perform 5 experiments under the same experimental conditions, and the preset number of times for each experiment is: 0, 5, 10, 15, and 20 times respectively.

[0047] Step 3, tissue fluid extraction: Use alcohol to clean the tissue fluid extraction site. After the alcohol evaporates, add 50 μL of PBS solution to the tissue fluid extraction site. Then, apply the conductive side of the electrode to the tissue fluid extraction site and start extracting tissue fluid using the AC current source designed in step 2. Step 4, tissue fluid detection: Collect tissue fluid from the working electrode surface and quantitatively dilute it. The diluted tissue fluid is then tested for sodium ion concentration, and the tissue fluid extraction rate is calculated based on the sodium ion concentration. It should be noted that a higher sodium ion concentration indicates a higher tissue fluid extraction rate.

[0048] like Figure 4 As shown in the figure, based on the experiment corresponding to Example 1 above, it can be seen that as the preset number of times increases, the sodium ion concentration in the tissue fluid gradually increases, and after the preset number of times exceeds 10, the sodium ion concentration in the tissue fluid tends to be stable. Therefore, the preset number of times is 10, which can meet the requirements of tissue fluid extraction.

[0049] Example 2: Step 1: Print the carbon black and nano-gold mixed conductive paste onto the substrate surface by screen printing. The working area of ​​the two printed electrodes is , then put it into an oven and dry it at 120℃ for 1h; Step 2, DC current source design: output a DC current signal with a DC current amplitude of 150 μA and a DC current duration of 40 s. Perform 5 experiments under the same experimental conditions, and the preset number of times for each experiment is 0, 5, 10, 15, and 20 times respectively.

[0050] Step 3, tissue fluid extraction: Use alcohol to clean the tissue fluid extraction site. After the alcohol evaporates, add 50 μL of PBS solution to the tissue fluid extraction site. Then, apply the conductive side of the electrode to the tissue fluid extraction site and use the DC current source designed in step 2 to start tissue fluid extraction. Step 4, tissue fluid detection: The tissue fluid on the working electrode surface is collected and quantitatively diluted. The sodium ion concentration of the diluted tissue fluid is then detected, and the tissue fluid extraction rate is calculated based on the sodium ion concentration.

[0051] like Figure 4 As shown, based on the experiments corresponding to the above-mentioned embodiment 1 and embodiment 2, it can be seen that under the same preset times, the tissue fluid extraction efficiency of the direct current signal is lower than that of the alternating current signal.

[0052] Example 3: Step 1: Print the carbon black and nano-gold mixed conductive paste onto the substrate surface by screen printing. The working area of ​​the two printed electrodes is , then put it into an oven and dry it at 120℃ for 1h; Step 2, AC current source design: output a sinusoidal AC signal with a preset time interval of 10s, a preset number of times, a variation amplitude of 10μA, a frequency of 20Hz, and a duration of 30s. Four experiments were performed under the same experimental conditions, and the corresponding reference current amplitudes for each experiment were 100μA, 150μA, 200μA, and 250μA, respectively.

[0053] Step 3, tissue fluid extraction: Use alcohol to clean the tissue fluid extraction site. After the alcohol evaporates, add 50 μL of PBS solution to the tissue fluid extraction site. Then, apply the conductive side of the electrode to the tissue fluid extraction site and start extracting tissue fluid using the AC current source designed in step 2. Step 4, tissue fluid detection: The tissue fluid on the working electrode surface is collected and quantitatively diluted. The sodium ion concentration of the diluted tissue fluid is then detected, and the tissue fluid extraction rate is calculated based on the sodium ion concentration.

[0054] like Figure 5 As shown in the experiment corresponding to Example 3 above, it can be seen that as the reference current amplitude of the AC signal output by the AC current source increases, the tissue fluid extraction rate increases. However, after the reference current amplitude exceeds 150 μA, the rate of increase in sodium ion concentration gradually slows. Therefore, to prevent excessive current from causing excessive irritation to human skin, a reference current amplitude of 150 μA is selected.

[0055] Example 4: Step 1: Print the carbon black and nano-gold mixed conductive paste onto the substrate surface by screen printing. The working area of ​​the two printed electrodes is , then put it into an oven and dry it at 120℃ for 1h; Step 2, AC current source design: output a sinusoidal AC signal with a preset time interval of 10s, a preset number of times of 10 times, a frequency of 20Hz, a reference current amplitude of 150μA, and a duration of the AC signal of 30s. Six experiments were performed under the same experimental conditions, and the corresponding amplitude changes for each experiment were 0μA, 10μA, 20μA, 30μA, 40μA and 50μA respectively.

[0056] Step 3, tissue fluid extraction: Use alcohol to clean the tissue fluid extraction site. After the alcohol evaporates, add 50 μL of PBS solution to the tissue fluid extraction site. Then, apply the conductive side of the electrode to the tissue fluid extraction site and start extracting tissue fluid using the AC current source designed in step 2. Step 4, tissue fluid detection: The tissue fluid on the working electrode surface is collected and quantitatively diluted. The sodium ion concentration of the diluted tissue fluid is then detected, and the tissue fluid extraction rate is calculated based on the sodium ion concentration.

[0057] like Figure 6 As shown, based on the experiment corresponding to the above embodiment 4, it can be seen that the sodium ion concentration is the largest when the change amplitude is 20 μA.

[0058] Comparative Example 1: Step 1: Print the carbon black and nano-gold mixed conductive paste onto the substrate surface by screen printing. The working area of ​​the two printed electrodes is , then put it into an oven and dry it at 120℃ for 1h; Step 2: Design a DC current source with a current amplitude of 150 μA and a duration of 400 ms. Step 3, tissue fluid extraction: Use alcohol to clean the tissue fluid extraction site. After the alcohol evaporates, add 50 μL of PBS solution to the tissue fluid extraction site. Then, apply the conductive side of the electrode to the tissue fluid extraction site and use the DC current source designed in step 2 to start tissue fluid extraction. Step 4, tissue fluid detection: The tissue fluid on the working electrode surface is collected and quantitatively diluted. The sodium ion concentration of the diluted tissue fluid is then detected, and the tissue fluid extraction rate is calculated based on the sodium ion concentration.

[0059] Comparative Example 2: Step 1: Print the carbon black and nano-gold mixed conductive paste onto the substrate surface by screen printing. The working area of ​​the two printed electrodes is , then put it into an oven and dry it at 120℃ for 1h; Step 2, AC current source design: output a preset time interval of 10s, set the base current amplitude of the AC signal to 150μA, the variation amplitude to 10μA, the frequency to 20Hz, and set the duration of the AC signal to a sinusoidal AC signal of 30s. Perform 5 experiments under the same experimental conditions, and the preset number of times for each experiment is: 0, 5, 10, 15, and 20 times respectively.

[0060] Step 3, tissue fluid extraction: Use alcohol to clean the tissue fluid extraction site. After the alcohol evaporates, add 50 μL of PBS solution to the tissue fluid extraction site. Then, apply the conductive side of the electrode to the tissue fluid extraction site and start extracting tissue fluid using the AC current source designed in step 2. Step 4, tissue fluid detection: The tissue fluid on the working electrode surface is collected and quantitatively diluted. The sodium ion concentration of the diluted tissue fluid is then detected, and the tissue fluid extraction rate is calculated based on the sodium ion concentration.

[0061] The experiment found that the skin stinging occurred in Comparative Example 1 after 150 seconds, which shows that the DC signal can cause trauma to the skin. Comparative Example 2 did not experience skin stinging within the preset number of 20 times.

[0062] The present application may be a system, method and / or computer program product. The computer program product may include a computer-readable storage medium carrying computer-readable program instructions for causing a processor to implement various aspects of the present application.

[0063] A computer-readable storage medium can be a tangible device that can hold and store instructions for use by an instruction execution device. A computer-readable storage medium can be, for example, but not limited to, an electrical storage device, a magnetic storage device, an optical storage device, an electromagnetic storage device, a semiconductor storage device, or any suitable combination thereof. More specific examples (a non-exhaustive list) of computer-readable storage media include: a portable computer disk, a hard disk, random access memory (RAM), read-only memory (ROM), erasable programmable read-only memory (EPROM or flash memory), static random access memory (SRAM), a portable compact disc read-only memory (CD-ROM), a digital versatile disk (DVD), a memory stick, a floppy disk, a mechanical encoding device, such as a punch card or raised-in-groove structure on which instructions are stored, and any suitable combination thereof. As used herein, a computer-readable storage medium is not to be construed as a transient signal per se, such as a radio wave or other freely propagating electromagnetic wave, an electromagnetic wave propagating through a waveguide or other transmission medium (e.g., a light pulse through a fiber optic cable), or an electrical signal transmitted through an electrical wire.

[0064] The computer-readable program instructions described herein can be downloaded from a computer-readable storage medium to each computing / processing device, or downloaded to an external computer or external storage device via a network, such as the Internet, a local area network, a wide area network, and / or a wireless network. The network can include copper transmission cables, fiber optic transmission, wireless transmission, routers, firewalls, switches, gateway computers, and / or edge servers. The network adapter card or network interface in each computing / processing device receives the computer-readable program instructions from the network and forwards the computer-readable program instructions to be stored in the computer-readable storage medium in each computing / processing device.

[0065] The computer program instructions for performing the operations of the present application may be assembly instructions, instruction set architecture (ISA) instructions, machine instructions, machine-dependent instructions, microcode, firmware instructions, state setting data, or source code or object code written in any combination of one or more programming languages, including object-oriented programming languages ​​such as Smalltalk, C++, and conventional procedural programming languages ​​such as "C" or similar programming languages. The computer-readable program instructions may be executed entirely on the user's computer, partially on the user's computer, as a stand-alone software package, partially on the user's computer and partially on a remote computer, or entirely on a remote computer or server. In the case of a remote computer, the remote computer may be connected to the user's computer via any type of network, including a local area network (LAN) or a wide area network (WAN), or may be connected to an external computer (e.g., via the Internet using an Internet service provider). In some embodiments, the state information of the computer-readable program instructions is used to personalize an electronic circuit, such as a programmable logic circuit, a field programmable gate array (FPGA), or a programmable logic array (PLA), so that the electronic circuit can execute the computer-readable program instructions, thereby implementing various aspects of the present application.

[0066] Various aspects of the present application are described herein with reference to flowcharts and / or block diagrams of methods, apparatus (systems), and computer program products according to embodiments of the present application. It should be understood that each block of the flowcharts and / or block diagrams, and combinations of blocks in the flowcharts and / or block diagrams, can be implemented by computer-readable program instructions.

[0067] These computer-readable program instructions can be provided to a processor of a general-purpose computer, a special-purpose computer, or other programmable data processing device, thereby producing a machine, so that when these instructions are executed by the processor of the computer or other programmable data processing device, a device is generated that implements the functions / actions specified in one or more blocks in the flowchart and / or block diagram. These computer-readable program instructions can also be stored in a computer-readable storage medium, where these instructions cause the computer, programmable data processing device, and / or other device to operate in a specific manner. Thus, the computer-readable medium storing the instructions comprises an article of manufacture that includes instructions for implementing various aspects of the functions / actions specified in one or more blocks in the flowchart and / or block diagram.

[0068] Computer-readable program instructions may also be loaded onto a computer, other programmable data processing apparatus, or other device so that a series of operational steps are performed on the computer, other programmable data processing apparatus, or other device to produce a computer-implemented process, thereby causing the instructions executed on the computer, other programmable data processing apparatus, or other device to implement the functions / actions specified in one or more blocks in the flowchart and / or block diagram.

[0069] The flowcharts and block diagrams in the accompanying drawings show the possible architecture, functions and operations of the systems, methods and computer program products according to multiple embodiments of the present application. In this regard, each box in the flowchart or block diagram can represent a part of a module, program segment or instruction, and the part of the module, program segment or instruction contains one or more executable instructions for realizing the specified logical function. In some alternative implementations, the functions marked in the box can also occur in an order different from that marked in the accompanying drawings. For example, two consecutive boxes can actually be executed substantially in parallel, and they can sometimes be executed in the opposite order, depending on the functions involved. It should also be noted that each box in the block diagram and / or flowchart, and the combination of the boxes in the block diagram and / or flowchart, can be implemented by a dedicated hardware-based system that performs the specified function or action, or can be implemented by a combination of dedicated hardware and computer instructions. It is well known to those skilled in the art that implementation by hardware, implementation by software, and implementation by a combination of software and hardware are all equivalent.

[0070] The embodiments of the present application have been described above. The above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terms used herein are selected to best explain the principles of the embodiments, practical applications, or technical improvements to technologies in the market, or to enable other persons skilled in the art to understand the embodiments disclosed herein. The scope of this application is defined by the appended claims.

Claims

1. A tissue fluid extraction device, characterized in that: include: An alternating current source and electrodes, wherein: The output end of the alternating current source is connected to the electrode, and is configured to output a preset alternating current signal to the electrode for a preset number of times according to a preset time interval, wherein the preset time interval is greater than 0, and the preset number of times is greater than or equal to 2; The electrodes are used to apply electrical stimulation corresponding to the AC current signal output by the AC current source to the tissue fluid extraction site, and the tissue fluid extraction site releases tissue fluid under the electrical stimulation.

2. The tissue fluid extraction device according to claim 1, characterized in that: The tissue fluid extraction device further includes: a detection element and a processor, wherein: The detection member is used to detect whether the electrode is attached to the tissue fluid extraction site; The processor is respectively connected to the output end of the detection component and the control end of the AC current source, and is used to control the AC current source to be in a working state of outputting a set AC current signal to the electrode for a preset number of times according to a preset time interval when the detection component determines that the electrode is applied to the tissue fluid extraction site.

3. The tissue fluid extraction device according to claim 2, characterized in that: The tissue fluid extraction device further comprises: a conductive liquid dripping member, wherein: The control end of the conductive liquid dripping component is connected to the processor, and the processor is also used to control the conductive liquid dripping component to drip conductive liquid to the tissue fluid extraction site before controlling the AC current source to be in a working state of outputting a set AC current signal to the electrode for a preset number of times according to a preset time interval when it is determined by the detection component that the electrode is applied to the tissue fluid extraction site.

4. The tissue fluid extraction device according to claim 3, characterized in that: The tissue fluid extraction device further comprises: a cleaning liquid dripping device, wherein: The control end of the cleaning liquid dripping component is connected to the processor, and the processor is also used to control the cleaning liquid dripping component to drip cleaning liquid to the tissue fluid extraction site before controlling the conductive liquid dripping component to drip conductive liquid to the tissue fluid extraction site when it is determined by the detection component that the electrode is applied to the tissue fluid extraction site.

5. The tissue fluid extraction device according to claim 1, characterized in that: The reference current amplitude of the AC current signal has a value range of [100, 250] μA, the variation amplitude has a value range of [10, 50] μA, and the frequency has a value range of [5, 200] Hz.

6. The tissue fluid extraction device according to claim 5, characterized in that: The AC current signal has a reference current amplitude of 150 μA, a variation amplitude of 20 μA, and a frequency of 20 Hz.

7. The tissue fluid extraction device according to claim 1, characterized in that: The preset time interval ranges from (0 to 30]s.

8. The tissue fluid extraction device according to claim 7, characterized in that: The preset time interval is 10s.

9. The tissue fluid extraction device according to claim 1, characterized in that: The range of the preset number of times is [5, 20].

10. The tissue fluid extraction device according to claim 9, characterized in that: The preset number of times is 10 times.

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