Anti-wrinkle, firming and repairing composition and preparation method thereof

Abstract

The invention discloses an anti-wrinkle, firming and repairing composition and a preparation method thereof, and belongs to the technical field of compositions. The haematococcus pluvialis extract rich in astaxanthin is obtained by utilizing an ionic liquid wall breaking agent to assist a low-temperature and high-pressure extraction technology, and the repairing effect of the haematococcus pluvialis extract is improved. Compared with simple physical mixing, the aggregate compound formed by compounding and assembling small molecule peptides (tripeptide-1, pentapeptide-3 and acetyl hexapeptide-8) and polyphenol-containing extracts (ginseng root extract and Chinese ganoderma lucidum extract) through interaction can improve the anti-wrinkle, firming and repairing effects; the haematococcus pluvialis extract, the olive fruit oil, the simmondsia chinensis seed oil, the camellia-seed oil, the butyrospermum parkii butter, the Angelica rotundifolia root essential oil, the lavandula officinalis oil and the lemongrass leaf oil are compounded and cooperated, so that the anti-wrinkle, firming and repairing effects are comprehensively upgraded.

Description

Technical Field

[0001] The present invention relates to the technical field of compositions, and in particular to an anti-wrinkle, firming and repairing composition and a preparation method thereof. Background Art

[0002] As we age, the metabolic rate of our cells gradually decreases. This reduced metabolism of skin cells can lead to roughness, dryness, sagging, and wrinkles. However, human skin aging is primarily caused by both internal and external factors. Endogenous factors primarily involve collagen loss with aging, resulting in reduced skin elasticity and sagging. Exogenous factors primarily involve environmental factors. Long-term exposure to harmful factors such as pollutants, radiation, and ultraviolet rays can damage cells and tissues, accelerating aging. With improved quality of life, people are increasingly interested in alleviating facial wrinkles and sagging. However, most traditional anti-aging cosmetics tend to focus solely on the aging process, lacking a systematic approach to addressing the wrinkles and sagging associated with aging. While existing anti-wrinkle and firming compositions using Haematococcus pluvialis extract as a raw material exist, the extraction efficiency of these extracts is poor, and extracts rich in astaxanthin are difficult to obtain, resulting in poor repair effects for these compositions. Summary of the Invention

[0003] The purpose of the present invention is to provide an anti-wrinkle, firming and repairing composition and a preparation method thereof, so as to solve the problem that the compositions for alleviating wrinkles and sagging caused by skin aging in the prior art have poor repairing effects.

[0004] The purpose of the present invention can be achieved through the following technical solutions: A method for preparing an anti-wrinkle, firming and repairing composition comprises the following steps: S1. Take tripeptide-1, pentapeptide-3, acetyl hexapeptide-8 and deionized water, mix them, and vortex mix to obtain material A; S2, taking ginseng root extract, Chinese ganoderma lucidum extract, deionized water and anhydrous ethanol, mixing and stirring to obtain material B; S3. Slowly inject the material A into the material B, and stir at room temperature at a speed of 120-180 r / min for 1-2 h to obtain material C; S4, taking squalane, cocoa seed butter, polydimethylsiloxane, olive fruit oil, jojoba seed oil, dimer linoleyl dimer linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, tri(laureth-4) phosphate, camellia seed oil, shea butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, polydimethylsiloxane crosspolymer, triethoxycaprylylsilane, angelica root essential oil, lavender oil, lemongrass leaf oil, Haematococcus pluvialis extract, and 1,2-pentanediol, and stirring at 50-60° C. for 30-50 min to obtain material D; S5. Mix deionized water, pearl powder, CI77891, material C, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, and panthenol, and stir at 40-50° C. for 20-30 minutes to obtain material E. S6. Mix the material D and the material E, and shear and homogenize them at 8000-12000 r / min for 3-5 minutes to obtain the anti-wrinkle, firming and repairing composition.

[0005] As a further solution of the present invention, in step S1, the mass ratio of the tripeptide-1, pentapeptide-3, acetyl hexapeptide-8 and deionized water is 5-7:8-12:15-20:500.

[0006] As a further embodiment of the present invention, in step S2, the mass ratio of the ginseng root extract, the Chinese ganoderma lucidum extract, the deionized water and the anhydrous ethanol is 15-17:8-12:500:100.

[0007] As a further solution of the present invention, in step S3, the mass ratio of material A to material B is 7:3.

[0008] As a further embodiment of the present invention, in step S4, the squalane, cocoa seed butter, polydimethylsiloxane, olive oil, jojoba seed oil, dimer linoleyl dimer linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, tri(laureth-4) phosphate, camellia seed oil, avocado tree butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, polydimethylsiloxane crosspolymer, triethoxycaprylylsilane, round-leaved angelica root essential oil, miscellaneous The mass ratio of lavender oil, lemongrass leaf oil, Haematococcus pluvialis extract and 1,2-pentanediol is 1-2:2-3:0.2-0.3:3-5:4-6:0.1-0.2:0.01-0.03:0.04-0.06:0.01-0.02:2-3:4-5:0.1-0.2:8-10:0.1-0.2:3-5:0.4-0.6:8-10:8-10:8-10:18-26:8-10.

[0009] As a further embodiment of the present invention, in step S5, the mass ratio of the deionized water, pearl powder, CI77891, material C, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, and panthenol is 70-80:3-5:2-4:14-16:3-5:2-3:4-5:6-9:2-3.

[0010] As a further solution of the present invention, in step S6, the mass ratio of material D to material E is 2-3:7-8.

[0011] As a further embodiment of the present invention, the preparation method of the Haematococcus pluvialis extract comprises the following steps: A1. Mix Haematococcus pluvialis powder, a cell-breaking agent, and deionized water, stir well, and place in a constant temperature oscillation box. Shake at 50° C. for 1-2 h, filter, retain the solid phase, and wash to obtain Haematococcus pluvialis powder after cell-breaking pretreatment. A2, the Haematococcus pluvialis powder after the pretreatment of the broken wall is mixed with an extraction solvent, uniformly dispersed by a high-speed disperser, injected into a high-pressure crushing and extraction device for crushing and extraction, centrifuged, and the liquid phase was rotary evaporated. The extracts were combined, concentrated under reduced pressure, and dried to obtain the Haematococcus pluvialis extract.

[0012] As a further embodiment of the present invention, the mass ratio of the Haematococcus pluvialis powder, the wall-breaking agent and the deionized water is 0.1-0.2:10-20:10-20.

[0013] As a further solution of the present invention, the ratio of the Haematococcus pluvialis powder to the extraction solvent is 1 g: 10-40 mL.

[0014] As a further embodiment of the present invention, the extraction solvent is selected from any one of methanol, anhydrous ethanol, and acetone.

[0015] As a further solution of the present invention, the conditions for the crushing extraction are a pressure of 50-200 MPa, a temperature of 0-4° C., and 1-3 extraction times.

[0016] As a further embodiment of the present invention, the preparation method of the wall-breaking agent comprises the following steps: Betaine, halogenated hydrocarbons, and anhydrous ethanol are mixed evenly, condensed and refluxed at 70° C. with stirring for 8-10 hours, filtered, the liquid phase is rotary evaporated to remove the anhydrous ethanol, dichloromethane is added, and the mixture is stirred at room temperature for 10-20 hours for reverse salting out to remove excess halogenated hydrocarbons, filtered, and the liquid phase is rotary evaporated to obtain a yellow viscous liquid, dichloromethane and ethyl acetate are added, stirred evenly, and then placed at -30° C. for recrystallization for 20-30 hours, filtered, and the solid phase is vacuum dried to obtain the wall-breaking agent.

[0017] As a further embodiment of the present invention, the proportion ratio of the betaine, the halogenated hydrocarbon, the anhydrous ethanol, the first added dichloromethane, the second added dichloromethane, and the ethyl acetate is 4-6 g: 3-5 g: 30-50 mL: 20-30 mL: 50 mL: 50 mL.

[0018] As a further embodiment of the present invention, the halogenated hydrocarbon is selected from any one of 1-bromopropane, 1-bromobutane, n-pentyl bromide, 1-bromohexane, chloropropane, 1-chlorobutane, 1-chlorohexane, 1-chloroheptane and 1-chlorooctane.

[0019] The anti-wrinkle, firming and repairing composition is prepared by the above preparation method.

[0020] Beneficial effects of the present invention: The present invention obtains an astaxanthin-rich Haematococcus pluvialis extract by utilizing an ionic liquid wall-breaking agent-assisted low-temperature and high-pressure extraction technology, thereby improving the repairing effect of the Haematococcus pluvialis extract.

[0021] Furthermore, the aggregate complex formed by the interaction and assembly of small molecule peptides (tripeptide-1, pentapeptide-3, acetyl hexapeptide-8) and polyphenol extracts (ginseng root extract, Chinese ganoderma lucidum extract) can improve the anti-wrinkle and firming repair effect compared with simple physical mixing; Furthermore, the chlorogenic acid and geniposide in gardenia extract are good at scavenging hydroxyl free radicals and singlet oxygen, while the polyphenols and cinnamic acid in cinnamon extract are more capable of scavenging superoxide anions and lipid peroxidation products. The combination of the two can form a "broad-spectrum antioxidant network", reducing the damage of free radicals to skin cells, lipids and proteins, and delaying the formation of wrinkles. Furthermore, the combination of Haematococcus pluvialis extract, olive oil, jojoba seed oil, camellia seed oil, avocado butter, angelica root oil, lavender oil, and lemongrass leaf oil synergistically delivers excellent anti-wrinkle, firming, and repairing benefits. The core active ingredient in Haematococcus pluvialis extract is natural astaxanthin, which effectively scavenges reactive oxygen species and lipid free radicals from the skin, reducing oxidative damage to collagen and elastin in the dermis caused by free radicals. This delays oxidative skin sagging and wrinkle deepening, promotes collagen synthesis, improves skin elasticity, inhibits the release of inflammatory factors, reduces skin aging caused by chronic inflammation, and promotes keratinocyte proliferation, assisting with barrier repair. Olive oil, jojoba seed oil, camellia seed oil, and avocado butter serve as a foundational care carrier, providing moisturizing, barrier support, and active ingredient delivery for anti-wrinkle repair. Olive oil is rich in olive polyphenols, vitamin E, and unsaturated fatty acids, which can synergize with astaxanthin to enhance antioxidant effects, reduce collagen oxidation, enhance the skin barrier's ability to lock in moisture, and reduce dry fine lines. Vitamin E stabilizes the astaxanthin structure and delays its oxidative degradation. Jojoba seed oil's main components are phytosterols, vitamin E, and long-chain fatty acid esters that are highly similar to skin sebum. It has strong skin affinity and can quickly penetrate and fill skin lipid gaps, regulate sebum secretion, and act as a fat-soluble carrier to promote the transdermal absorption of active ingredients such as astaxanthin and improve bioavailability. Camellia seed oil contains tea polyphenols, vitamin E, unsaturated fatty acids, and squalene, which form antioxidant complementarity with astaxanthin, reducing water loss and fine lines caused by barrier damage. Avocado tree butter is rich in triglycerides, phytosterols, and fat-soluble vitamins, providing a stable environment for other active ingredients.

[0022] Angelica sinensis root essential oil contains angelica lactones and terpenes, which can promote local blood circulation in the skin, enhance the efficiency of the dermis in delivering nutrients (such as astaxanthin and collagen synthesis raw materials), and accelerate the repair of damaged tissues; at the same time, its mild penetration-promoting effect can assist the penetration of fat-soluble ingredients and improve the utilization rate of efficacy; lavender oil is rich in lavandulol, linalyl acetate, etc., which can relieve chronic skin inflammation, while soothing the slight irritation that may be caused by highly active ingredients such as astaxanthin, and improving the mildness of the product; lemongrass leaf oil contains citronellal, geraniol, etc., which have antioxidant, antibacterial and skin microenvironment regulation effects. Its antioxidant capacity can supplement the basic antioxidant network and indirectly assist in anti-wrinkle repair.

[0023] During skin aging, the accumulation of free radicals can lead to cell damage and collagen degeneration. Astaxanthin from Haematococcus pluvialis is a powerful free radical scavenger that can directly quench singlet oxygen and lipid peroxidation free radicals. Olive polyphenols from olive oil and tea polyphenols from camellia seed oil are good at scavenging hydroxyl free radicals and superoxide anions. The broad-spectrum antioxidant network formed by the combination of the three reduces the aging damage of free radicals to the skin from the source. Astaxanthin activates fibroblasts to synthesize type I collagen, and the unsaturated fatty acids in olive oil provide raw materials for collagen synthesis. Angelica sinensis essential oil can improve circulation and Rapid nutrition supply, synergistically improve the efficiency of collagen production; the anti-inflammatory ingredients of lavender oil and lemongrass oil can reduce skin inflammatory response and reduce inflammation-mediated aging; jojoba seed oil and olive oil as carriers can increase the solubility and transdermal efficiency of fat-soluble ingredients such as astaxanthin, making it easier for active ingredients to reach the dermis and exert their effects; the use of Haematococcus pluvialis extract, olive oil, jojoba seed oil, camellia seed oil, avocado tree butter, round-leaf angelica root essential oil, lavender oil, and lemongrass leaf oil in a complex and synergistic combination has achieved a comprehensive upgrade in anti-wrinkle, firming and repairing effects. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention are clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative work are within the scope of protection of the present invention.

[0025] Preparation Example 1 The preparation method of the Haematococcus pluvialis extract comprises the following steps: A1, take Haematococcus pluvialis powder, wall-breaking agent and deionized water, mix, stir evenly and place in a constant temperature shaking box, shake at 50 ° C for 1 hour, filter, retain the solid phase, wash, obtain Haematococcus pluvialis powder after wall-breaking pretreatment; The mass ratio of the Haematococcus pluvialis powder, the wall-breaking agent and deionized water is 0.1:10:10; The preparation method of the wall-breaking agent comprises the following steps: Betaine, 1-bromobutane, and anhydrous ethanol were mixed evenly, condensed and refluxed at 70°C with stirring for 8 hours, filtered, and the liquid phase was rotary evaporated to remove the anhydrous ethanol, dichloromethane was added, and the mixture was stirred at room temperature for 10 hours to perform reverse salting out to remove excess halogenated hydrocarbons, filtered, and the liquid phase was rotary evaporated to obtain a yellow viscous liquid, dichloromethane and ethyl acetate were added, stirred evenly, and then placed at -30°C for recrystallization for 20 hours, filtered, and the solid phase was vacuum dried to obtain the wall-breaking agent; The dosage ratio of betaine, 1-bromobutane, anhydrous ethanol, dichloromethane added for the first time, dichloromethane added for the second time, and ethyl acetate is 4 g: 3 g: 30 mL: 20 mL: 50 mL: 50 mL; A2, the Haematococcus pluvialis powder after the pretreatment of the broken wall was mixed with anhydrous ethanol, and after being uniformly dispersed by a high-speed disperser, injected into a high-pressure crushing and extraction device for crushing and extraction, centrifuged, and the liquid phase was rotary evaporated. The extracts were combined, concentrated under reduced pressure, and dried to obtain the Haematococcus pluvialis extract; The ratio of the Haematococcus pluvialis powder to anhydrous ethanol is 1 g:10 mL; the crushing and extraction conditions are a pressure of 50 MPa, a temperature of 4° C., and two extractions.

[0026] Preparation Example 2 The preparation method of the Haematococcus pluvialis extract comprises the following steps: A1, take Haematococcus pluvialis powder, wall-breaking agent and deionized water, mix, stir evenly and place in a constant temperature shaking box, shake at 50 ° C for 1.5h, filter, retain the solid phase, wash, and obtain Haematococcus pluvialis powder after wall-breaking pretreatment; The mass ratio of the Haematococcus pluvialis powder, the wall-breaking agent and deionized water is 0.15:15:15; The preparation method of the wall-breaking agent comprises the following steps: Betaine, 1-bromobutane, and anhydrous ethanol were mixed evenly, condensed and refluxed at 70°C with stirring for 9 hours, filtered, and the liquid phase was rotary evaporated to remove the anhydrous ethanol, dichloromethane was added, and the mixture was stirred at room temperature for 15 hours to perform reverse salting out to remove excess halogenated hydrocarbons, filtered, and the liquid phase was rotary evaporated to obtain a yellow viscous liquid, dichloromethane and ethyl acetate were added, stirred evenly, and then placed at -30°C for recrystallization for 25 hours, filtered, and the solid phase was vacuum dried to obtain the wall-breaking agent; The dosage ratio of betaine, 1-bromobutane, anhydrous ethanol, dichloromethane added for the first time, dichloromethane added for the second time, and ethyl acetate is 5 g: 4 g: 40 mL: 25 mL: 50 mL: 50 mL; A2, the Haematococcus pluvialis powder after the pretreatment of the broken wall was mixed with anhydrous ethanol, and after being uniformly dispersed by a high-speed disperser, injected into a high-pressure crushing and extraction device for crushing and extraction, centrifuged, and the liquid phase was rotary evaporated. The extracts were combined, concentrated under reduced pressure, and dried to obtain the Haematococcus pluvialis extract; The ratio of the Haematococcus pluvialis powder to anhydrous ethanol is 1 g: 25 mL; the crushing and extraction conditions are a pressure of 150 MPa, a temperature of 4° C., and two extractions.

[0027] Preparation Example 3 The preparation method of the Haematococcus pluvialis extract comprises the following steps: A1, take Haematococcus pluvialis powder, wall-breaking agent and deionized water, mix, stir evenly and place in a constant temperature shaking box, shake at 50 ° C for 2h, filter, retain the solid phase, wash, obtain Haematococcus pluvialis powder after wall-breaking pretreatment; The mass ratio of the Haematococcus pluvialis powder, the wall-breaking agent and deionized water is 0.2:20:20; The preparation method of the wall-breaking agent comprises the following steps: Betaine, 1-bromobutane, and anhydrous ethanol were mixed evenly, condensed and refluxed at 70°C with stirring for 10 hours, filtered, and the liquid phase was rotary evaporated to remove the anhydrous ethanol, dichloromethane was added, and the mixture was stirred at room temperature for 20 hours to perform reverse salting out to remove excess halogenated hydrocarbons, filtered, and the liquid phase was rotary evaporated to obtain a yellow viscous liquid, dichloromethane and ethyl acetate were added, stirred evenly, and then placed at -30°C for recrystallization for 30 hours, filtered, and the solid phase was vacuum dried to obtain the wall-breaking agent; The dosage ratio of betaine, 1-bromobutane, anhydrous ethanol, dichloromethane added for the first time, dichloromethane added for the second time, and ethyl acetate is 6 g: 5 g: 50 mL: 30 mL: 50 mL: 50 mL; A2, the Haematococcus pluvialis powder after the pretreatment of the broken wall was mixed with anhydrous ethanol, and after being uniformly dispersed by a high-speed disperser, injected into a high-pressure crushing and extraction device for crushing and extraction, centrifuged, and the liquid phase was rotary evaporated. The extracts were combined, concentrated under reduced pressure, and dried to obtain the Haematococcus pluvialis extract; The ratio of the Haematococcus pluvialis powder to anhydrous ethanol is 1 g:40 mL; the crushing and extraction conditions are a pressure of 200 MPa, a temperature of 4° C., and two extractions.

[0028] Preparation Example 4 The difference from Preparation Example 2 is that the preparation method of the Haematococcus pluvialis extract includes the following steps: The Haematococcus pluvialis powder is mixed with anhydrous ethanol, uniformly dispersed in a high-speed disperser, injected into a high-pressure crushing and extraction device for crushing and extraction, centrifuged, and the liquid phase is rotary evaporated. The extracts are combined, concentrated under reduced pressure, and dried to obtain the Haematococcus pluvialis extract. The ratio of the Haematococcus pluvialis powder to anhydrous ethanol is 1 g: 25 mL; the crushing and extraction conditions are a pressure of 150 MPa, a temperature of 4° C., and two extractions.

[0029] The total astaxanthin content of the Haematococcus pluvialis extracts obtained in Preparation Examples 1 to 4 was determined by ultraviolet spectrophotometry and the yield was calculated. The results are shown in Table 1 below.

[0030] Table 1 As shown in Table 1, the present invention uses ionic liquid as a wall-breaking agent to assist in pre-treating the wall of Haematococcus pluvialis. The ionic liquid in the wall-breaking agent coordinates the OH in the cellulose. - , thereby weakening the hydrogen bond interaction between cellulose chains, showing better solubility for cellulose, effectively destroying the cell wall of Haematococcus pluvialis, increasing the extraction rate of astaxanthin, the anti-wrinkle ingredient in Haematococcus pluvialis, increasing the content of anti-wrinkle ingredients in the raw materials of Haematococcus pluvialis extract, and improving the anti-wrinkle effect.

[0031] The Haematococcus pluvialis extract obtained in Preparation Example 2 was used as the raw material for the following examples and comparative examples.

[0032] Example 1 A method for preparing an anti-wrinkle, firming and repairing composition comprises the following steps: S1. Mix tripeptide-1, pentapeptide-3, acetyl hexapeptide-8 and deionized water, and vortex mix to obtain material A; the mass ratio of tripeptide-1, pentapeptide-3, acetyl hexapeptide-8 and deionized water is 5:8:15:500; S2, taking ginseng root extract, Chinese ganoderma lucidum extract, deionized water and anhydrous ethanol, mixing and stirring uniformly to obtain material B; the mass ratio of the ginseng root extract, Chinese ganoderma lucidum extract, deionized water and anhydrous ethanol is 15:8:500:100; S3. Slowly inject the material A into the material B, and stir at room temperature at a speed of 120 r / min for 1 hour to obtain material C; the mass ratio of the material A to the material B is 7:3; S4, take squalane, cocoa seed butter, polydimethylsiloxane, olive oil, jojoba seed oil, dimer linoleyl dimer linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, tri(laureth-4) phosphate, camellia seed oil, shea butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, polydimethylsiloxane crosspolymer, triethoxycaprylylsilane, round-leaved angelica root essential oil, lavender oil, lemongrass leaf oil, Haematococcus pluvialis extract, 1,2-pentanediol, mix, stir at 50 ° C for 30 min, obtain material D; the squalane, cocoa seed butter, polydimethylsiloxane, olive The mass ratio of jojoba oil, simmondsia chinensis seed oil, dimer dimer dimer linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, trilaureth-4 phosphate, camellia seed oil, shea butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, dimethicone crosspolymer, triethoxycaprylylsilane, angelica rotundifolia root essential oil, lavender oil, lemongrass leaf oil, Haematococcus pluvialis extract, and 1,2-pentanediol is 1:2:0.2:3:4:0.1:0.01:0.04:0.01:2:4:0.1:8:0.1:3:0.4:8:8:8:18:8; S5. Deionized water, pearl powder, CI77891, material C, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, and panthenol were mixed and stirred at 40°C for 20 minutes to obtain material E; the mass ratio of deionized water, pearl powder, CI77891, material C, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, and panthenol was 70:3:2:14:3:2:4:6:2; S6. Mix the material D and the material E, and shear and homogenize them at 8000 r / min for 5 minutes to obtain the anti-wrinkle, firming and repairing composition; the mass ratio of the material D to the material E is 3:7.

[0033] Example 2 A method for preparing an anti-wrinkle, firming and repairing composition comprises the following steps: S1. Mix tripeptide-1, pentapeptide-3, acetyl hexapeptide-8 and deionized water, and vortex mix to obtain material A; the mass ratio of tripeptide-1, pentapeptide-3, acetyl hexapeptide-8 and deionized water is 6:10:18:500; S2, taking ginseng root extract, Chinese ganoderma lucidum extract, deionized water and anhydrous ethanol, mixing and stirring uniformly to obtain material B; the mass ratio of the ginseng root extract, Chinese ganoderma lucidum extract, deionized water and anhydrous ethanol is 16:10:500:100; S3. Slowly inject the material A into the material B, and stir at room temperature at a speed of 150 r / min for 1.5 h to obtain material C; the mass ratio of the material A to the material B is 7:3; S4, take squalane, cocoa seed butter, polydimethylsiloxane, olive fruit oil, jojoba seed oil, dimer linoleyl dimer linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, tri(laureth-4) phosphate, camellia seed oil, shea butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, polydimethylsiloxane crosspolymer, triethoxycaprylylsilane, round-leaved angelica root essential oil, lavender oil, lemongrass leaf oil, Haematococcus pluvialis extract, 1,2-pentanediol, mix, stir at 55 ° C for 40 min, obtain material D; the ...ate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, tri(laureth-4) phosphate, camellia seed oil, shea butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, polydimethylsiloxane crosspolymer, triethoxycaprylylsilane The mass ratio of seed oil, dimerized dimerized dimerized linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, trilaureth-4 phosphate, camellia seed oil, shea butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, dimethicone crosspolymer, triethoxycaprylylsilane, angelica rotundifolia root essential oil, lavender oil, lemongrass leaf oil, Haematococcus pluvialis extract, and 1,2-pentanediol is 1.5:2.5:0.25:4:5:0.15:0.02:0.05:0.015:2.5:4.5:0.15:9:0.15:4:0.5:9:9:9:22:9; S5. Deionized water, pearl powder, CI77891, material C, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, and panthenol were mixed and stirred at 45° C. for 25 min to obtain material E; the mass ratio of deionized water, pearl powder, CI77891, material C, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, and panthenol was 75:4:3:15:4:2.5:4.5:8:2.5; S6. Mix the material D and the material E, and homogenize them at 10,000 r / min for 4 minutes to obtain the anti-wrinkle, firming and repairing composition; the mass ratio of the material D to the material E is 2:8.

[0034] Example 3 A method for preparing an anti-wrinkle, firming and repairing composition comprises the following steps: S1. Take tripeptide-1, pentapeptide-3, acetyl hexapeptide-8 and deionized water, mix them, and vortex mix them to obtain material A; the mass ratio of the tripeptide-1, pentapeptide-3, acetyl hexapeptide-8 and deionized water is 7:12:20:500; S2, taking ginseng root extract, Chinese ganoderma lucidum extract, deionized water and anhydrous ethanol, mixing and stirring uniformly to obtain material B; the mass ratio of the ginseng root extract, Chinese ganoderma lucidum extract, deionized water and anhydrous ethanol is 17:12:500:100; S3. Slowly inject the material A into the material B, and stir at room temperature at a speed of 180 r / min for 2 h to obtain material C; the mass ratio of the material A to the material B is 7:3; S4, take squalane, cocoa seed butter, polydimethylsiloxane, olive oil, jojoba seed oil, dimer linoleyl dimer linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, tri(laureth-4) phosphate, camellia seed oil, shea butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, polydimethylsiloxane crosspolymer, triethoxycaprylylsilane, angelica root essential oil, lavender oil, lemongrass leaf oil, Haematococcus pluvialis extract, 1,2-pentanediol, mix, stir at 60 ° C for 50 min, obtain material D; the squalane, cocoa seed butter, polydimethylsiloxane, olive oil , jojoba seed oil, dimer dimer dimer linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, tri(laureth-4) phosphate, camellia seed oil, shea butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, dimethicone crosspolymer, triethoxycaprylylsilane, angelica rotundifolia root essential oil, lavender oil, lemongrass leaf oil, Haematococcus pluvialis extract, and 1,2-pentanediol in a mass ratio of 2:3:0.3:5:6:0.2:0.03:0.06:0.02:3:5:0.2:10:0.2:5:0.6:10:10:10:26:10; S5. Deionized water, pearl powder, CI77891, material C, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, and panthenol were mixed and stirred at 50° C. for 30 min to obtain material E; the mass ratio of deionized water, pearl powder, CI77891, material C, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, and panthenol was 80:5:4:16:5:3:5:9:3; S6. Mix the material D and the material E, and shear and homogenize them at 12000 r / min for 3 min to obtain the anti-wrinkle, firming and repairing composition; the mass ratio of the material D to the material E is 2.5:7.5.

[0035] Comparative Example 1 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that the component does not contain tripeptide-1, and its reduced amount is distributed to pentapeptide-3 and acetyl hexapeptide-8 in proportion to the number of parts, while the other components and amounts remain unchanged.

[0036] Comparative Example 2 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that the composition does not contain pentapeptide-3, and its reduced amount is distributed to tripeptide-1 and acetyl hexapeptide-8 in proportion to the number of parts. The other components and amounts remain unchanged.

[0037] Comparative Example 3 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that the composition does not contain acetyl hexapeptide-8, and its reduced amount is distributed to tripeptide-1 and pentapeptide-3 in proportion to the number of parts, while the other components and amounts remain unchanged.

[0038] Comparative Example 4 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that the composition does not contain ginseng root extract, and the reduced amount thereof is distributed to the Chinese Ganoderma lucidum extract in proportion to the number of parts, while the other components and amounts remain unchanged.

[0039] Comparative Example 5 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that the Chinese Ganoderma lucidum extract is not included in the composition, and the reduced amount thereof is distributed to the ginseng root extract in proportion to the number of parts, while the other components and amounts remain unchanged.

[0040] Comparative Example 6 This comparative example provides an anti-wrinkle, firming and repairing composition. The composition differs from Example 2 only in that the composition does not contain gardenia extract, and its reduced amount is distributed to cinnamon extract in proportion to the number of parts, while the other components and amounts remain unchanged.

[0041] Comparative Example 7 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that the composition does not contain cinnamon extract, and the reduced amount thereof is distributed to the gardenia extract in proportion to the number of parts, while the other components and amounts remain unchanged.

[0042] Comparative Example 8 The difference from Example 2 is that the preparation method of the anti-wrinkle, firming and repairing composition comprises the following steps: S1. According to the formula, squalane, cocoa seed butter, polydimethylsiloxane, olive oil, jojoba seed oil, dimer linoleyl dimer linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, tri(laureth-4) phosphate, camellia seed oil, shea butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, polydimethylsiloxane crosspolymer, triethoxycaprylylsilane, angelica root essential oil, lavender oil, lemongrass leaf oil, Haematococcus pluvialis extract, and 1,2-pentanediol were mixed and stirred at 55° C. for 40 min to obtain material D; S2. According to the formula, deionized water, pearl powder, CI77891, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, panthenol, tripeptide-1, pentapeptide-3, acetyl hexapeptide-8, ginseng root extract, and Chinese ganoderma extract were mixed and stirred at 45°C for 25 minutes to obtain material E; S6. According to the formula, take the material D and the material E, mix them, and shear them at 10000 r / min for 4 minutes to obtain the anti-wrinkle, firming and repairing composition.

[0043] Comparative Example 9 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that the composition does not contain olive oil. The reduced amount of olive oil is distributed according to the proportion of parts to jojoba seed oil, camellia seed oil, avocado tree butter, angelica root essential oil, lavender oil, lemongrass leaf oil, and Haematococcus pluvialis extract. The other components and amounts remain unchanged.

[0044] Comparative Example 10 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that jojoba seed oil is not included in the components. The reduced amount is distributed according to the proportion of parts to olive oil, camellia seed oil, avocado tree butter, angelica root essential oil, lavender oil, lemongrass leaf oil, and Haematococcus pluvialis extract. The other components and amounts remain unchanged.

[0045] Comparative Example 11 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that camellia seed oil is not included in the components. The reduced amount is distributed according to the proportion of parts among olive oil, jojoba seed oil, avocado tree fruit butter, angelica root essential oil, lavender oil, lemongrass leaf oil, and Haematococcus pluvialis extract. The other components and amounts remain unchanged.

[0046] Comparative Example 12 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that avocado butter is not included in the components. The reduced amount is distributed according to the proportion of parts among olive oil, jojoba seed oil, camellia seed oil, angelica root essential oil, lavender oil, lemongrass leaf oil, and Haematococcus pluvialis extract. The other components and amounts remain unchanged.

[0047] Comparative Example 13 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that the composition does not contain angelica root essential oil. The reduced amount is distributed according to the proportion of parts to olive oil, jojoba seed oil, camellia seed oil, avocado tree fruit butter, lavender oil, lemongrass leaf oil, and Haematococcus pluvialis extract, while the other components and amounts remain unchanged.

[0048] Comparative Example 14 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that lavender oil is not included in the components. The reduced amount is distributed according to the proportion of parts to olive oil, jojoba seed oil, camellia seed oil, avocado tree butter, angelica root essential oil, lemongrass leaf oil, and Haematococcus pluvialis extract. The other components and amounts remain unchanged.

[0049] Comparative Example 15 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that lemongrass leaf oil is not included in the components. The reduced amount is distributed according to the proportion of parts to olive oil, jojoba seed oil, camellia seed oil, avocado tree butter, angelica root essential oil, lavender oil, and Haematococcus pluvialis extract. The other components and amounts remain unchanged.

[0050] Comparative Example 16 This comparative example provides an anti-wrinkle, firming and repairing composition. The only difference between the composition and Example 2 is that the component does not contain Haematococcus pluvialis extract. The reduced amount is distributed according to the proportion of parts to olive oil, jojoba seed oil, camellia seed oil, avocado tree butter, angelica root essential oil, lavender oil, and lemongrass leaf oil. The other components and amounts remain unchanged.

[0051] Performance Testing Test Example 1 Anti-wrinkle effect test This test refers to the laboratory method (HMC-WI-028 elastase inhibition rate) and compares the elastase inhibition test results of the test sample with the negative control. If the inhibition rate of the test sample is higher than that of the negative control and the difference is significant (P<0.05), it can be considered that the test sample has certain anti-wrinkle efficacy. The test results are shown in Table 2 below.

[0052] Table 2 As can be seen from Table 2, the anti-wrinkle, firming and repairing compositions prepared in Examples 1 to 3 of the present application have good anti-wrinkle efficacy and exhibit significant elastase inhibition effects.

[0053] According to the comparison of the data of Example 2 with Comparative Examples 1 to 5 and Comparative Example 8, it can be seen that the complex formed by the combination and assembly of small molecule peptides (tripeptide-1, pentapeptide-3, acetyl hexapeptide-8) and polyphenol-containing extracts (ginseng root extract, Chinese Ganoderma lucidum extract) has good anti-wrinkle effect.

[0054] According to the comparison of the data of Example 2 and Comparative Examples 6-7, it can be seen that the combination of Gardenia jasminoides extract and Cinnamon bark extract synergistically exerts a good anti-wrinkle effect.

[0055] According to the data comparison of Example 2 and Comparative Examples 9 to 16, it can be seen that the compounding of Haematococcus pluvialis extract, olive oil, jojoba seed oil, camellia seed oil, avocado tree butter, angelica root essential oil, lavender oil, and lemongrass leaf oil synergistically exerts a good anti-wrinkle effect.

[0056] Test Example 2 Repair Effect Test 80 healthy Chinese female subjects with dry, red, fragile, sensitive, rough and obvious wrinkles on their faces were selected, with an age range of 28-58 years old. The subjects were divided into 16 groups, 5 people in each group. The subjects cleaned their faces with facial cleanser, sat quietly for 30 minutes, performed a half-face test on their skin, applied the products of Example 1-Example 3 and Comparative Example 1-Comparative Example 16, massaged to 30 minutes after absorption, and then tested their skin again. Statistical analysis was performed on the changes before and after the skin, and the rates of change of various indicators were obtained. The test results are shown in Table 3 below.

[0057] Table 3 As can be seen from Table 3, the anti-wrinkle and firming repair compositions prepared in Examples 1 to 3 of the present application have better anti-wrinkle and firming repair effects than those in Comparative Examples 1 to 16.

[0058] The above describes some embodiments of the present invention in detail, but the contents described are only preferred embodiments of the present invention and should not be considered to limit the scope of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. A method for preparing an anti-wrinkle, firming and repairing composition, characterized in that: The following steps are involved: S1. Take tripeptide-1, pentapeptide-3, acetyl hexapeptide-8 and deionized water, mix them, and vortex mix to obtain material A; S2, taking ginseng root extract, Chinese ganoderma lucidum extract, deionized water and anhydrous ethanol, mixing and stirring to obtain material B; S3. Slowly inject the material A into the material B, and stir at room temperature at a speed of 120-180 r / min for 1-2 h to obtain material C; S4, taking squalane, cocoa seed butter, polydimethylsiloxane, olive fruit oil, jojoba seed oil, dimer linoleyl dimer linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, tri(laureth-4) phosphate, camellia seed oil, shea butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, polydimethylsiloxane crosspolymer, triethoxycaprylylsilane, angelica root essential oil, lavender oil, lemongrass leaf oil, Haematococcus pluvialis extract, and 1,2-pentanediol, and stirring at 50-60° C. for 30-50 min to obtain material D; S5. Mix deionized water, pearl powder, CI77891, material C, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, and panthenol, and stir at 40-50° C. for 20-30 minutes to obtain material E. S6. Mix the material D and the material E, and shear and homogenize them at 8000-12000 r / min for 3-5 minutes to obtain the anti-wrinkle, firming and repairing composition.

2. The method for preparing an anti-wrinkle, firming and repairing composition according to claim 1, characterized in that: In step S1, the mass ratio of the tripeptide-1, pentapeptide-3, acetyl hexapeptide-8 and deionized water is 5-7:8-12:15-20:

500.

3. The method for preparing an anti-wrinkle, firming and repairing composition according to claim 1, characterized in that: In step S2, the mass ratio of the ginseng root extract, the Chinese ganoderma lucidum extract, deionized water and anhydrous ethanol is 15-17:8-12:500:

100.

4. The method for preparing an anti-wrinkle, firming and repairing composition according to claim 1, characterized in that: In step S3, the mass ratio of material A to material B is 7:

3.

5. The method for preparing an anti-wrinkle, firming and repairing composition according to claim 1, characterized in that: In step S4, the squalane, cocoa seed butter, polydimethylsiloxane, olive oil, jojoba seed oil, dimer linoleyl dimer linoleate, sorbitan sesquioleate, PEG-30 dipolyhydroxystearate, tri(laureth-4) phosphate, camellia seed oil, avocado tree butter, magnesium stearate, 1,2-hexanediol, p-hydroxyacetophenone, polydimethylsiloxane crosspolymer, triethoxycaprylylsilane, round-leaved angelica root essential oil, lavender oil, lemon The mass ratio of citronella leaf oil, Haematococcus pluvialis extract and 1,2-pentanediol is 1-2:2-3:0.2-0.3:3-5:4-6:0.1-0.2:0.01-0.03:0.04-0.06:0.01-0.02:2-3:4-5:0.1-0.2:8-10:0.1-0.2:3-5:0.4-0.6:8-10:8-10:8-10:18-26:8-10.

6. The method for preparing the anti-wrinkle, firming and repairing composition according to claim 1, characterized in that: In step S5, the mass ratio of the deionized water, pearl powder, CI77891, material C, gardenia extract, cinnamon extract, niacinamide, glycerin, butylene glycol, and panthenol is 70-80:3-5:2-4:14-16:3-5:2-3:4-5:6-9:2-3.

7. The method for preparing an anti-wrinkle, firming and repairing composition according to claim 1, characterized in that: In step S6, the mass ratio of material D to material E is 2-3:7-8.

8. The method for preparing the anti-wrinkle, firming and repairing composition according to claim 1, characterized in that: The preparation method of the Haematococcus pluvialis extract comprises the following steps: A1. Mix Haematococcus pluvialis powder, a cell-breaking agent, and deionized water, stir well, and place in a constant temperature oscillation box. Shake at 50° C. for 1-2 h, filter, retain the solid phase, and wash to obtain Haematococcus pluvialis powder after cell-breaking pretreatment. A2, the Haematococcus pluvialis powder after the pretreatment of the broken wall is mixed with an extraction solvent, uniformly dispersed by a high-speed disperser, injected into a high-pressure crushing and extraction device for crushing and extraction, centrifuged, and the liquid phase was rotary evaporated. The extracts were combined, concentrated under reduced pressure, and dried to obtain the Haematococcus pluvialis extract.

9. The method for preparing the anti-wrinkle, firming and repairing composition according to claim 8, characterized in that: The preparation method of the wall-breaking agent comprises the following steps: Betaine, halogenated hydrocarbons, and anhydrous ethanol are mixed evenly, condensed and refluxed at 70° C. with stirring for 8-10 hours, filtered, the liquid phase is rotary evaporated to remove the anhydrous ethanol, dichloromethane is added, and the mixture is stirred at room temperature for 10-20 hours for reverse salting out to remove excess halogenated hydrocarbons, filtered, and the liquid phase is rotary evaporated to obtain a yellow viscous liquid, dichloromethane and ethyl acetate are added, stirred evenly, and then placed at -30° C. for recrystallization for 20-30 hours, filtered, and the solid phase is vacuum dried to obtain the wall-breaking agent.

10. The anti-wrinkle, firming and repairing composition prepared according to the preparation method according to any one of claims 1 to 9.

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