Method for extracting gingerol at low temperature by using ginger roots

Abstract

The invention belongs to the field of organic matter extraction, and particularly relates to a method for low-temperature extraction of gingerol from ginger roots. The method comprises the following steps: crushing raw materials, preparing an extracting solution, and extracting gingerol. According to the method, an alcohol-acid system is introduced to replace a traditional single alcohol solution, and gingerol extraction under the low-temperature condition is achieved.

Description

Technical Field

[0001] The invention belongs to the field of organic matter extraction, and particularly relates to a method for extracting gingerol from ginger root at low temperature. Background Art

[0002] Ginger is the fresh rhizome of the perennial monocotyledonous herb Zingiber officinale Roscoe, cultivated as an annual. Its rhizome is large and contains volatile oils. The rhizome (dried ginger) has a pungent flavor and is used as a vegetable, spice, and medicine. It is warm in nature, and its unique gingerol can stimulate the gastrointestinal mucosa, causing congestion and enhancing digestion. It is effective in treating abdominal distension, pain, diarrhea, and vomiting caused by excessive consumption of cold foods. It also has antioxidant and anti-allergic properties, and can lower blood and liver cholesterol.

[0003] There are currently many methods for extracting gingerol from ginger, including boiling, column chromatography, organic solvent extraction and physical methods. However, all four methods have defects:

[0004] 1) The boiling method takes a long time and consumes more energy. Due to the long heating time, the active ingredients are more damaged, so the product quality is poor;

[0005] 2) Column chromatography is difficult to use for mass production due to limitations in eluent and elution volume;

[0006] 3) Organic solvent extraction method, according to the principle of like dissolves like, extracts gingerol, because the solubility of solvent is limited, in order to improve the extraction rate, mainly increases the amount of solvent, along with the increase of the amount of solvent, consumption also increases thereupon, cost is bound to rise, so economic benefit often shows negative growth, cannot really put into production, can only stay in the experimental stage of laboratory; As anhydrous ethylene glycol is used to extract gingerol, the extraction rate of gingerol slowly improves along with the increase of anhydrous ethylene glycol consumption, therefore often there is the extraction rate of gingerol and improves, but simultaneously the loss of anhydrous ethylene glycol also significantly increases, in addition the technological process is longer, therefore energy consumption is more, solvent loss increases, production cost rises, final economic benefit usually shows negative growth, cannot really put into production, can only stay in laboratory;

[0007] 4) Physical methods can be divided into three types according to different processing methods: freezing method, microwave method and ultrasonic method; these three methods can play a certain auxiliary role in extraction, but at the same time increase energy consumption and production costs. Summary of the Invention

[0008] To solve the above problems, the present invention provides a method for extracting gingerol, comprising the following steps:

[0009] (1) Sample preparation: Select ginger roots that are free of mold and remove dirt, impurities, and contaminants from the surface to obtain the sample to be extracted;

[0010] (2) Preparation of the extract: Take anhydrous ethylene glycol and add distilled water to prepare an ethylene glycol solution; then take concentrated hydrochloric acid and add it to the above ethylene glycol solution, mix thoroughly, and obtain an extract;

[0011] (3) Gingerol extraction: The sample to be extracted and the extract are mixed and ground to obtain a homogenate, the homogenate is ultrasonically treated, the homogenate after ultrasonic treatment is centrifuged and the supernatant is collected, and the supernatant is freeze-dried to obtain gingerol.

[0012] Furthermore, the concentration of the ethylene glycol solution in step (2) is 20%.

[0013] Furthermore, the concentration of concentrated hydrochloric acid in step (2) is 36% to 38% by volume.

[0014] Furthermore, the volume ratio of the ethylene glycol solution to concentrated hydrochloric acid in step (2) is 100:0.1.

[0015] Furthermore, in step (3), the ratio of the sample to be extracted to the extract is 0.6 g:10 mL.

[0016] Furthermore, the ultrasonic treatment conditions in step (3) are ultrasonic extraction at 25° C. for 30 min, with an ultrasonic frequency of 40 kHz and a power of 200 W.

[0017] Furthermore, the centrifugation condition in step (3) is 5000 r / min for 10 minutes.

[0018] The present invention has the following beneficial effects:

[0019] The method of the present invention not only extracts crude gingerol quickly but also maintains its original active state, reducing the amount of gingerol used by more than 80% compared to conventional extraction methods. It overcomes the shortcomings of water boiling, column chromatography, organic solvent extraction, and physical methods, which are high energy consumption and difficult to scale up, thereby significantly reducing costs. DETAILED DESCRIPTION

[0020] Various exemplary embodiments of the present invention are now described in detail. Unless otherwise specified, the methods in the examples are conventional methods. Unless otherwise specified, the reagents used are conventional commercially available reagents or reagents prepared using conventional methods. This detailed description should not be considered a limitation of the present invention, but rather should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0021] It should be understood that the terms described herein are intended only to describe particular embodiments and are not intended to limit the present invention. In addition, for numerical ranges herein, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. The intermediate value within any stated value or stated range, and each smaller range between any other stated value or intermediate value within the stated range, is also encompassed within the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded within the scope.

[0022] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.

[0023] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from the description of the invention. The description and examples are intended to be illustrative only.

[0024] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0025] Example 1 Extraction of gingerol from ginger root

[0026] 1.1 Sample preparation

[0027] Sample Preparation: Select plump, mold-free ginger root. Clean any dirt, impurities, and contaminants from the surface. Accurately weigh 0.6g of ginger root and place in a mortar.

[0028] 1.2 Preparation of extract

[0029] Extraction solution composition: 0.1% hydrochloric acid in 40% ethylene glycol aqueous solution.

[0030] Preparation method: Take 20mL of anhydrous ethylene glycol and add 60mL of distilled water to make a 20% ethylene glycol solution.

[0031] Then take 0.1 mL of concentrated hydrochloric acid (volume fraction of concentrated hydrochloric acid is 36% to 38%), add it to the above 20% ethylene glycol solution, and mix thoroughly to obtain the extract.

[0032] 1.3 Sample extraction

[0033] Preliminary grinding: Add 2 mL of extract solution to a mortar and pestle and grind the ginger root thoroughly until the sample becomes a uniform paste.

[0034] Mortar cleaning: Clean the mortar and pestle with 2 mL of the extract and flush the remaining ginger root slurry into a 10 mL centrifuge tube.

[0035] Ultrasonic-assisted extraction: Place the centrifuge tube in an ultrasonic chamber and perform ultrasonic extraction for 30 min at 25°C. The ultrasonic frequency is set to 40 kHz and the power is 200 W.

[0036] Centrifugation: After extraction, place the sample in a centrifuge at 5000 rpm for 10 minutes. Collect the supernatant and transfer it to a clean 10 mL centrifuge tube.

[0037] Repeat the extraction: Repeat the ultrasonic extraction and centrifugation process on the centrifuged sediment three times using fresh extraction solution. Collect the supernatant each time and combine the supernatants from the three extractions.

[0038] 1.4 Volume determination and storage

[0039] The combined supernatant was transferred to a 10 mL volumetric flask and diluted to 10 mL with an extractant (0.1% hydrochloric acid in 20% ethylene glycol). The diluted extract was transferred to a sealed container and stored at -20°C to prevent degradation of gingerol and maintain its stability.

[0040] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A method for extracting gingerol, characterized in that, The following steps are involved: (1) Sample preparation: Select ginger roots that are free of mold and remove dirt, impurities, and contaminants from the surface to obtain the sample to be extracted; (2) Preparation of the extract: Take anhydrous ethylene glycol and add distilled water to prepare an ethylene glycol solution; then take concentrated hydrochloric acid and add it to the above ethylene glycol solution, mix thoroughly, and obtain an extract; (3) Gingerol extraction: The sample to be extracted and the extract are mixed and ground to obtain a homogenate, the homogenate is ultrasonically treated, the homogenate after ultrasonic treatment is centrifuged and the supernatant is collected, and the supernatant is freeze-dried to obtain gingerol.

2. The method according to claim 1, characterized in that The concentration of the ethylene glycol solution in step (2) is 20%.

3. The method according to claim 1, characterized in that The concentration of concentrated hydrochloric acid in step (2) is 36% to 38% by volume.

4. The method according to claim 1, wherein The volume ratio of the ethylene glycol solution and concentrated hydrochloric acid in step (2) is 100:0.

1.

5. The method according to claim 1, wherein The ratio of the sample to be extracted to the extract in step (3) is 0.6 g:10 mL.

6. The method according to claim 1, wherein The ultrasonic treatment conditions in step (3) are ultrasonic extraction at 25° C. for 30 min, with an ultrasonic frequency of 40 kHz and a power of 200 W.

7. The method according to claim 1, characterized in that The centrifugal condition in step (3) is 5000 r / min for 10 minutes.