Psychrobacter sp. Capable of efficiently degrading biogenic amine and application of psychrobacter sp.

Abstract

The invention discloses a psychrobacter capable of efficiently degrading biogenic amine and application of the psychrobacter. The strain is a strain FJNU-SIO36, the classification name of the strain is Psychrobacter sp., the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is January 6, 2025, the preservation number is CGMCC No.33316, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing. The strain FJNU-SIO36 does not generate biogenic amines, can degrade nine biogenic amines such as putrescine, cadaverine, histamine and tyramine at the same time, and especially has a degradation rate of 100% for putrescine and cadaverine, which is of great significance to degradation of biogenic amines in fermented food. The strain FJNU-SIO36 provided by the invention can be used for degrading biogenic amines, and is wide in application range, low in cost and simple to operate.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and particularly relates to a psychrophilic bacillus capable of efficiently degrading biogenic amines and an application thereof. Background Art

[0002] Biogenic amines (BAs) are low-molecular-weight, nitrogenous alkaline compounds whose formation mechanisms primarily involve amino acid decarboxylation, aldehyde-ketone amination, and transamination. BAs are widely present in various protein-rich food systems, with particularly high levels found in seafood, meat, and fermented products. Aquatic products, rich in protein, provide amino acid precursors for microbial metabolism during storage and processing to synthesize BAs. Notably, consuming high concentrations of BAs can severely impact health, with symptoms including headaches, nausea, and difficulty breathing.

[0003] Aquatic products, such as oysters, are prone to spoilage during storage and preservation, producing common biocides (BAs) such as histamine, putrescine, cadaverine, tyramine, tryptamine, phenylethylamine, spermine, spermidine, and octopamine, which can affect food quality and safety. Controlling BAs in food has been a hot topic of research in recent years. Current approaches to BA control focus on two main areas: first, utilizing amine oxidases to degrade formed BAs; and second, screening for BA-free strains with amine-degrading activity to replace amine-producing bacteria to reduce BA production during fermentation. Studies have shown that yeast (CN111493271 A), Bacillus subtilis (CN 115161222B), and Lactobacillus (CN 115612642 B) all exhibit the ability to degrade biogenic amines. However, these strains suffer from limited amine-degrading efficacy and poor environmental adaptability.

[0004] There is a gap in the existing research on the development of BAs-degrading strains for special environments (high salt, low temperature and complex bacterial flora) such as aquatic product systems. Screening strains with high efficiency in amine degradation from aquatic products can not only effectively maintain the original flavor of the product, but also effectively control BAs and ensure food safety. Summary of the Invention

[0005] The present invention aims to provide a strain of Psychrophilic Bacillus FJNU-SIO36 that can efficiently degrade biogenic amines and its application in degrading biogenic amines.

[0006] A strain FJNU-SIO36 that efficiently degrades biogenic amines is classified and named Psychrobacter sp. and deposited in the General Microbiology Center (CGMCC) of the China Culture Collection Administration. The deposit date is January 6, 2025, the deposit number is CGMCC No. 33316, and the deposit address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0007] Strain FJNU-SIO36 was isolated from Pacific oysters farmed in Fujian Province. After being harvested from a coastal aquaculture farm in Fuzhou, the oysters were placed in a foam box with ice and transported to the laboratory within 4 hours. They were then refrigerated at 4°C for 4 days, and then screened and isolated from their gills. This strain exhibits a strong ability to degrade biogenic amines and was systematically identified as Psychrobacter sp.

[0008] The biogenic amine-degrading strain FJNU-SIO36 obtained through isolation and screening of the present invention was found to be unable to produce BAs after isolation and screening. The strain of the present invention was able to degrade 100% of putrescine and cadaverine after 24 hours in a culture environment with a concentration of 100 mg / L of each BA, demonstrating high efficiency in degrading putrescine and cadaverine. The strain also achieved a degradation rate of over 60% for spermidine and exhibited a certain degree of degradation for tyramine, tryptamine, phenylethylamine, octopamine, and spermidine.

[0009] The strain FJNU-SIO36 can be used to prepare a degradation bacterial agent for degrading biogenic amines.

[0010] Compared to existing technologies, the strain FJNU-SIO36 provided by the present invention does not produce biogenic amines itself, but can simultaneously degrade multiple biogenic amines. It is low-cost and simple to operate, making it of great significance for biogenic amine degradation. The strain provided by the present invention can be used to degrade biogenic amines in a wide range of applications, such as high-protein food wastewater and biological environmental wastewater. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 This is the single colony morphology of strain FJNU-SIO36 after dilution coating on a plate.

[0012] Figure 2 This is a phylogenetic tree constructed based on 16S rRNA sequences, in which Psychrobacterium FJNU-SIO36 is marked with ★.

[0013] Figure 3 These are the growth curve (A), acid resistance curve (B), and salt resistance curve (C) of strain FJNU-SIO36.

[0014] Figure 4This is a high-performance liquid chromatography (HPLC) graph of BAs produced by the strain; the peak numbers in the graph represent different biogenic amines: 1, tryptamine, 2, phenylethylamine, 3, putrescine, 4, cadaverine, 5, histamine, 6, octopamine, 7, internal standard, 8, tyramine, 9, spermidine, 10, spermine.

[0015] Figure 5 This is a HPLC graph of BAs degradation by the strain for 24 h; the peak numbers in the figure represent different biogenic amines: 1, tryptamine, 2, phenylethylamine, 3, putrescine, 4, cadaverine, 5, histamine, 6, octopamine, 7, internal standard, 8, tyramine, 9, spermidine, 10, spermine.

[0016] Figure 6 is the degradation rate of biogenic amines by strain FJNU-SIO36 at 12h, 24h, 36h and 48h.

[0017] Figure 7 The degradation rate of cadaverine (100 mg / L) by the strain FJNU-SIO36 at different times.

[0018] Figure 8 is the degradation rate of different concentrations of cadaverine by the bacterial strain FJNU-SIO36 in 24 hours.

[0019] Figure 9 Figure 3 is the ability of strain FJNU-SIO36 to degrade cadaverine under different salinity conditions (A) and acidic conditions (B). DETAILED DESCRIPTION

[0020] The present invention will be further described below with reference to the accompanying drawings and examples.

[0021] Unless otherwise specified, the raw materials and reagents in the present invention are commercially available.

[0022] Example 1 Isolation, screening and identification of strains

[0023] 1. Isolation and Screening of Strains

[0024] 1.1 Materials: Oysters were Pacific oysters farmed in Fujian Province. They were harvested from a coastal farm in Fuzhou City, placed in a foam box containing ice, and transported to the laboratory for processing within 4 h.

[0025] 1.2 Experimental culture medium

[0026] (1) LB liquid medium: 10 g / L tryptone, 5 g / L yeast powder, 10 g / L sodium chloride. Stir thoroughly and sterilize at 121°C for 20 min.

[0027] (2) LB-A liquid medium: 10 g / L tryptone, 5 g / L yeast powder, 10 g / L sodium chloride, 1 g of each amino acid (histidine, tyrosine, lysine, tryptophan, arginine, ornithine, and phenylalanine). Stir thoroughly and sterilize at 121°C for 20 min.

[0028] (3) PDA solid medium: potato 200g / L, glucose 20g / L, agar 15g / L

[0029] (4) NA solid medium: peptone 10 g / L, beef extract powder 3 g / L, sodium chloride 5 g / L, agar 15 g / L

[0030] (5) 2216E solid medium: peptone 5 g / L, yeast extract 1 g / L, ferric citrate 0.1 g / L, sodium chloride 19.45 g / L, magnesium chloride 5.98 g / L, sodium sulfate 3.24 g / L, calcium chloride 1.8 g / L, potassium chloride 0.55 g / L, sodium carbonate 0.16 g / L, potassium bromide 0.08 g / L, strontium chloride 0.034 g / L, boric acid 0.022 g / L, sodium silicate 0.004 g / L, sodium fluoride 0.0024 g / L, sodium nitrate 0.0016 g / L, disodium hydrogen phosphate 0.008 g / L, agar 15 g / L

[0031] 1.3 Isolation of strains

[0032] The gills and intestines of oysters stored for 4 days were collected for bacterial isolation. 10 g of sample was added to 90 mL of sterile saline and shaken for 20 minutes. 1 mL of sample was added to 9 mL of saline and mixed to prepare a bacterial suspension. This bacterial suspension was then diluted to prepare 6 gradient bacterial suspensions (10 -2 -10 -7 ), accurately absorb 10 -4 , 10 -5 and 10 -6 The bacterial suspensions were inoculated into PDA solid medium, NA solid medium, LB solid medium, and 2216E solid medium, respectively, and cultured at 28°C for 48 h. Single colonies were streaked three times for purification. A single colony of the purified strain FJNU-SIO36 was inoculated into LB liquid medium and cultured at 28°C, 220 rpm, for 12 h. The culture suspension was then diluted with 50% glycerol (v:v = 1:1) and stored at -80°C for further analysis.

[0033] 1.4 DNA extraction and PCR amplification of strains

[0034] Bacterial cultures grown overnight at 28°C were harvested, and genomic DNA from strain FJNU-SIO36 was extracted using the Trelief Genomic DNA Kit (MP Biomedicals). The extracted bacterial DNA was stored at −20°C until further use. The genomic DNA was used as a template to amplify bacterial 16S rRNA using universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3'), synthesized by Beijing Qingke Biotechnology Co., Ltd.

[0035] A 25 μL PCR amplification reaction system consisted of 1 μL of DNA template, 1 μL each of primers 27F and 1492R (10 μmol / L), and 12.5 μL of 2× San Taq PCR Mix (Shanghai Sangon Biotechnology Co., Ltd.), complete with ddH₂O. PCR reaction conditions were as follows: 94°C initial denaturation for 5 min; 34 cycles of denaturation at 94°C for 30 s, annealing at 49°C for 30 s, and extension at 72°C for 90 s; followed by an additional extension at 72°C for 10 min, and storage at 4°C. Amplified products were sequenced by Sanger sequencing, and the sequencing results were then compared with the NCBI database for Blast analysis.

[0036] 2. Identification of strains

[0037] The isolated strain was activated by streaking on LB medium, and a single colony was picked with an inoculating stick. The colony morphology was described, and the microscopic morphology was measured and described after slide preparation, as follows:

[0038] Morphological description of strain FJNU-SIO36: The morphology of the strain was observed after culturing on inorganic salt medium for 1 day, and the data were recorded. The colony morphology was round, the colony size was 0.5-1.5mm, the front was white, opaque, with neat edges, flat, and moist texture. Figure 1 ).

[0039] A bacterial suspension was prepared according to the MacF concentration required by the corresponding test card. The bacterial suspension was injected into the corresponding test card using a VITKE2 automatic identification instrument. The machine read the absorbance every 15 minutes, and the results were compared with the strain library in the system to issue a strain identification report. Based on the 16S rDNA sequence of the strain, sequence similarity evaluation and phylogenetic tree analysis were used. After Maximum Likelihood phylogenetic tree analysis, strain FJNU-SIO36 had a high homology with Psychrobacter sp. ( Figure 2 ).

[0040] BLASTn comparison of 16S rRNA sequences revealed a 95.41% similarity between strain FJNU-SIO36 and Psychrobacter sp. Based on morphological and molecular data, strain FJNU-SIO36 was identified as belonging to the psychrophilic bacterium Psychrobacter sp. This psychrophilic bacterium was deposited with the General Microbiology Center of the China General Microbiology Culture Collection (CGMCC) under the accession number CGMCC No. 33316 and on January 6, 2025.

[0041] 3. Preservation of strains

[0042] Strain FJNU-SIO36 was streaked onto LB medium plates and incubated upside down at 28°C for 2 days. Single colonies were then streaked three times for purification. A single colony of the purified strain was inoculated into LB liquid medium and incubated overnight at 28°C, 220 rpm. Under aseptic conditions, the culture was aspirated into sterile 50% glycerol (v:v = 1:1) cryovials and stored in a -80°C freezer. Survival was monitored regularly.

[0043] Example 2 Growth curve of strain FJNU-SIO36

[0044] The strain FJNU-SIO36 was cultured overnight and inoculated into LB medium at a 1% inoculum size. The culture was carried out at 28°C and 220 rpm. The absorbance of the bacterial suspension was measured at 600 nm every 2 hours. The OD 600 Indicates its biomass ( Figure 3 ).

[0045] The growth curve of strain FJNU-SIO36 was analyzed. The strain experienced a lag phase, a logarithmic phase and a stable phase. It entered the logarithmic phase at 15 hours and the stable phase at 35 hours.

[0046] Example 3 Acid resistance curve and salt resistance curve of strain FJNU-SIO36

[0047] The strain FJNU-SIO36 was cultured overnight and inoculated into LB medium at a 1% inoculum size at 28°C and 220 rpm. The effects of pH and NaCl concentration on the growth of the strain were analyzed. The strain was inoculated into LB liquid medium with pH values ​​of 4.0, 5.0, 6.0, 7.0, 8.0, and 9.0 and LB liquid medium with NaCl concentrations of 1%, 4%, 8%, 12%, 16%, and 20% at a 1% inoculum size. The OD values ​​were measured after 48 h. 600 ( Figure 3 ).

[0048] The experimental results showed that strain FJNU-SIO36 had the highest biomass at pH 6.0, decreased with increasing pH, and was unable to grow at pH 4.0, 5.0, and 10.0. The strain also had the highest biomass at a NaCl concentration of 4%, decreased with increasing NaCl concentration, and was unable to grow at concentrations above 16%.

[0049] Example 4 Determination of the ability of strain FJNU-SIO36 to produce bioamines

[0050] 1. Preparation of LB-A medium for biogenic amine fermentation: Weigh 10 g of tryptone, 5 g of yeast powder, 10 g of sodium chloride, and 1 g each of amino acids (histidine, tyrosine, lysine, tryptophan, arginine, ornithine, and phenylalanine), add distilled water and dilute to 1000 mL.

[0051] 2. Determination of bioamine production capacity: strain FJNU-SIO36 was cultured overnight and inoculated into LB-A medium at a 1% inoculum size. The culture was incubated at 28°C and 180 rpm for 24 h. The supernatant was collected by centrifugation at 10,000 rpm for 10 min, and 1 mL of the supernatant was used to analyze the BAs content.

[0052] 3. Detection of biogenic amines in fermentation broth:

[0053] Fermentation broth pretreatment: 2 mL of the culture broth collected above was centrifuged at 10,000 rpm for 10 min, and 1 mL of the supernatant was collected to prepare the test sample. The test sample was derivatized and then the biogenic amine content was determined by high performance liquid chromatography.

[0054] (1) Pre-column derivatization of biogenic amines

[0055] Mix 1 mL of the mixed standard BAs working solution or sample supernatant with 1 mL of saturated NaHCO₃ solution, 100 μL of NaOH solution (1 mol / L), 250 μL of the internal standard working solution, and 1 mL of a derivatizing agent (10 mg / mL). The reaction mixture is incubated in a 60°C water bath for 15 minutes. 0.5 mL of aqueous ammonia is then added to terminate the reaction, and the mixture is incubated in a 60°C water bath for 15 minutes. The sample is extracted with 3 mL of diethyl ether, and the supernatant is collected in a 10 mL test tube and dried. The residue is redissolved in 1 mL of acetonitrile, filtered through a 0.22 μm filter, and analyzed by high-performance liquid chromatography.

[0056] (2) Preparation of biogenic amine standard curve

[0057] A 100 mg / L mixed standard BAs working solution was diluted with 0.1 mol / L HCl solution to a concentration gradient of 1, 2.5, 5, 10, 15, 25, 50, and 100 mg / L to prepare a standard curve. The standard curve was plotted with the peak area of ​​each BAs divided by the peak area of ​​the internal standard as the Y-axis and the concentration gradient of each BAs as the x-axis.

[0058] (3) HPLC conditions

[0059] Chromatographic separation conditions: Derivatized BAs were detected using HPLC-UV (Waters e2695-2998, Waters, USA) and a reversed-phase C18 column (Waters Sunfire, 5 μm, 4.6 × 250 mm). A gradient elution rate of 0.8 mL / min was used for acetonitrile (mobile phase A) and water (mobile phase B). UV detection was at 254 nm. The gradient elution program is shown in Table 1.

[0060] Table 1. Elution schedule for RP-HPLC determination of biogenic amines

[0061]

[0062] 4. Results: BAs production capacity of strain FJNU-SIO36: No BAs were produced. Figure 4 .

[0063] Example 5 Degradation experiment of biogenic amines by strain FJNU-SIO36

[0064] 1. Preparation of LB-B medium for biogenic amine fermentation: Weigh 10 g of tryptone, 5 g of yeast powder, 10 g of sodium chloride, and 0.1 g each of biogenic amines (tryptamine, phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermine, and spermidine), and add distilled water to make the volume to 1000 mL.

[0065] 2. Laboratory amine degradation performance verification experiment: Strain FJNU-SIO36 was inoculated into 10 mL of LB liquid culture medium and cultured overnight at 28°C, 220 rpm. A 1% inoculum was then inoculated into LB-B medium and cultured at 28°C, 220 rpm for 24 h.

[0066] 3. Detection of biogenic amines in fermentation broth

[0067] 4. BAs degradation ability of strain FJNU-SIO36: After 24 h of culture, the degradation rates of tryptamine, phenylethylamine, putrescine, cadaverine, octopamine, tyramine, and spermidine were 26%, 14%, 100%, 100%, 2%, 13%, and 73% ( Figure 5The experiment showed that strain FJNU-SIO36 was able to completely degrade putrescine and cadaverine in a culture environment with a concentration of 100 mg / L of each BA, demonstrating its high efficiency in degrading putrescine and cadaverine, and also exhibited a certain degradation ability for other BAs except histamine and spermine.

[0068] After 12 h, 24 h, 36 h and 48 h of culture, the degradation rates of tryptamine, phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermine and spermidine were determined. Figure 6 The experiment showed that the psychrophilic bacterium FJNU-SIO36 had an outstanding ability to degrade cadaverine and putrescine, achieving 100% degradation within 24 hours.

[0069] Example 6 Ability of strain FJNU-SIO36 to degrade cadaverine

[0070] (1) Degradation rate of cadaverine by strain FJNU-SIO36 at different times

[0071] The strain FJNU-SIO36 was cultured overnight and inoculated into LB medium at a 1% inoculum size at 28°C and 220 rpm. The strain was inoculated into LB medium containing 100 mg / L cadaverine at a 1% inoculum size. The degradation rate of cadaverine by the strain was determined at 12 h, 24 h, 36 h and 48 h. Figure 7 ).

[0072] The results showed that the degradation rate of 100 mg / L cadaverine by the strain reached 100% within 24 hours.

[0073] (2) Degradation rate of strain FJNU-SIO36 on different concentrations of cadaverine

[0074] The strain FJNU-SIO36 was cultured overnight and inoculated into LB medium at a 1% inoculum size at 28°C and 220 rpm. The strain was inoculated into LB medium containing 100 mg / L, 200 mg / L, 300 mg / L, 400 mg / L and 500 mg / L cadaverine at a 1% inoculum size. The degradation rate of cadaverine by the strain was determined after 24 h. Figure 8 ).

[0075] The results showed that the strain had a strong ability to degrade cadaverine. After 24 hours, the strain could completely degrade 100 mg / L and 200 mg / L of cadaverine. Moreover, for LB culture medium containing 300 mg / L, 400 mg / L and 500 mg / L of cadaverine, the strain's degradation rate of cadaverine could reach more than 60%.

[0076] Example 7 Ability of strain FJNU-SIO36 to degrade cadaverine under different conditions

[0077] The strain FJNU-SIO36 was cultured overnight and inoculated into LB medium at a 1% inoculum size at 28°C and 220 rpm. The strain was then inoculated into liquid culture medium containing 100 mg / L cadaverine under different conditions at a 1% inoculum size. The ability of the strain to degrade cadaverine under different acidic and salinity conditions was analyzed, as well as the degradation rate of cadaverine at different times ( Figure 9 ).

[0078] (1) Ability to degrade cadaverine under different acidic conditions

[0079] The strain FJNU-SIO36 was cultured overnight and inoculated with 1% of the inoculum into liquid culture medium containing 100 mg / L cadaverine at pH 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0, respectively. The culture was carried out at 28°C and 220 rpm. Samples were taken after 24 hours to determine the degradation rate of cadaverine and the OD value. 600 .

[0080] The results showed that the strain had the highest cadaverine degradation rate at a pH of 6.0-9.0, reaching 100%. However, the strain's degradation rate was less than 10% under extremely acidic (pH 4.0 and 5.0) and extremely alkaline (pH 10.0) conditions. The strain's microbial activity was strongest under neutral conditions, while the microbial content was low under extremely acidic and alkaline conditions, indicating that the optimal pH for cadaverine degradation by the strain is 6.0-9.0.

[0081] (2) Ability to degrade cadaverine under different salinity conditions

[0082] The strain FJNU-SIO36 was cultured overnight and inoculated with 1% inoculum in liquid culture medium containing 100 mg / L cadaverine at NaCl concentrations of 1%, 4%, 8%, 12%, 16% and 20%, respectively. The culture was carried out at 28°C and 220 rpm. Samples were taken after 24 hours to determine the degradation rate of cadaverine and OD 600 .

[0083] The results showed that the strain's ability to degrade cadaverine decreased with the increase of NaCl concentration. When the NaCl concentration was 1% and 4%, the cadaverine degradation rate was the highest, reaching 100%.

[0084] The optimal salinity condition for the strain is 4% NaCl concentration, and its ability to degrade cadaverine can reach 100%.

[0085] Example 8 Preparation of a Degradation Bacterial Agent Based on Strain FJNU-SIO36

[0086] (1) The strain FJNU-SIO36 was streaked and isolated on an LB plate. After activation, a single clone was picked and inoculated into a test tube containing LB liquid medium and cultured until the exponential growth phase as a primary seed liquid; then, a 1% (v / v) inoculum was inoculated into LB liquid medium and cultured with shaking until the exponential growth phase to obtain a seed liquid for fermentation.

[0087] (2) The fermentation seed liquid prepared above is inoculated into the culture medium of the production tank at a concentration of 10-20% (v / v) and fermented for 12-24 hours. During the culture process, the pH is adjusted online to 7.0-7.4, the ventilation rate of sterile air is 1.0-1.5 vvm, the stirring rate is 200-300 rpm, and the culture temperature is 28°C. After the fermentation is completed, the culture liquid is discharged from the tank and directly packaged into a liquid degradation agent, or the bacteria are dried to form a powder, or prepared into a high-purity preparation through separation and purification steps. The culture medium of the production tank is LB liquid medium.

[0088] The above results show that strain FJNU-SIO36 has a good effect in degrading biogenic amines and does not produce biogenic amines itself.

[0089] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention shall still fall within the scope of the technical solution of the present invention.

Claims

1. A strain FJNU-SIO36 that efficiently degrades biogenic amines, characterized by: Its classification is named Psychrobacterium Psychrobacter sp.), deposited in the General Microbiology Center of China Culture Collection of Microorganisms, with a deposit date of January 6, 2025, and a deposit number of CGMCC No. 33316. The deposit address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

2. The strain FJNU-SIO36 for efficiently degrading biogenic amines according to claim 1, characterized in that: The nucleotide sequence of 16S rDNA of the strain FJNU-SIO36 is shown in SEQ ID No.

1.

3. A method for degrading biogenic amines, characterized in that: The method uses the strain FJNU-SIO36 as described in claim 1.

4. The method for degrading biogenic amines according to claim 3, characterized in that: The biogenic amine is tryptamine, phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine or spermidine.

5. The method for degrading biogenic amines according to claim 3, characterized in that: The reaction conditions for degrading biogenic amines are: pH=6.0~9.

0.

6. Use of the strain FJNU-SIO36 according to claim 1 in degrading biogenic amines.

7. Use of the strain FJNU-SIO36 according to claim 1 in preparing a biodegradation bacterial agent for degrading biogenic amines.

8. The use according to claim 6 or 7, characterized in that The biogenic amine is tryptamine, phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine or spermidine.

9. A biogenic amine-degrading bacterial agent, characterized in that: The strain FJNU-SIO36 is prepared by using the strain FJNU-SIO36 as claimed in claim 1.

Images