Head-mounted non-human primate cerebral cortex high-precision optical genetic stimulation equipment

Abstract

The invention discloses a head-mounted non-human primate cerebral cortex high-precision optical genetic stimulation device, which comprises a MicroLED display device, an optical genetic stimulation device, an optical genetic stimulation device and an optical genetic stimulation device, and is characterized in that the MicroLED display device is configured to provide image light and project a stimulation pattern; the imaging lens is configured to image stimulation patterns output by the MicroLED display device to the cerebral cortex of the non-human primate animal, the imaging lens is of a holosymmetric structure, and the imaging lens sequentially comprises a second lens group, a third lens group, a diaphragm, a fourth lens group and a fifth lens group in the light transmission direction; wherein the third lens group and the fourth lens group are symmetrical about the diaphragm, the second lens group and the fifth lens group are symmetrical about the diaphragm, the imaging magnification of the imaging lens is 1.0-1.1 times, the optical genetic stimulation equipment can project different patterns at different positions in a cerebral cortex target area according to requirements, the weight is light, and different brightness and colors can be set.

Description

Technical Field

[0001] The present application relates to the fields of optical configuration and biological experiment technology, and in particular to a head-mounted high-precision optogenetic stimulation device for the cerebral cortex of non-human primates. Background Art

[0002] Optogenetics, a technology that precisely modulates the activity of neurons expressing light-sensitive proteins using specific wavelengths of light, has become a revolutionary tool for understanding the function of neural circuits. In brain research using non-human primate (NHP) models (such as macaques and cynomolgus monkeys), this technology is invaluable for exploring higher-level cognitive functions and the mechanisms of brain disease.

[0003] However, traditional optogenetic stimulation technologies face significant challenges: 1) Fiber-based approaches primarily rely on optical fiber to transmit light signals, resulting in a single output spot morphology (typically limited to a circular shape). This lacks flexibility in spatial stimulation patterns, making it difficult to meet the diverse stimulation patterns and high-precision spatial positioning requirements of complex neural circuit studies. 2) Beam expansion and collimation combined with microlens arrays, while capable of generating multi-point stimulation, require multi-stage optical module coupling (beam expansion and collimation, microlens arrays, microscopic imaging, and integrated optical fiber), resulting in a complex and heavy system structure and significant aberrations that affect spot positioning accuracy and stimulation reliability. In summary, existing technologies struggle to achieve lightweight, high-precision, and programmable optogenetic stimulation in freely behaving non-human primates.

[0004] Therefore, there is an urgent need in this field to develop a head-mounted high-precision optogenetic stimulation device for the cerebral cortex of non-human primates, which can project different patterns at different locations in the target area of ​​the cerebral cortex according to needs, is lightweight and can be set to different brightness and colors. Summary of the Invention

[0005] The purpose of this application is to provide a head-mounted high-precision optogenetic stimulation device for the cerebral cortex of non-human primates, which can project different patterns at different locations in the area according to needs, and can set different brightness and colors.

[0006] This application provides a head-mounted high-precision optogenetic stimulation device for the cerebral cortex of non-human primates, comprising: a MicroLED display device configured to provide image light and project a stimulation pattern; An imaging lens configured to image the stimulation pattern output by the MicroLED display device onto the cerebral cortex of the non-human primate, wherein the imaging lens is a fully symmetrical structure and includes, in sequence along the light transmission direction, a second lens group, a third lens group, an aperture, a fourth lens group, and a fifth lens group. The third lens group and the fourth lens group are symmetrical about the aperture stop, and the second lens group and the fifth lens group are symmetrical about the aperture stop; The second lens group includes at least one lens, the second lens group has negative optical power, the third lens group includes a plurality of lenses, the third lens group has positive optical power and is used to converge light diverging through the second lens group into quasi-parallel light and correct spherical aberration; The fourth lens group includes multiple lenses, the fourth lens group has positive optical power, the fourth lens group and the third lens group are optically conjugate, the fifth lens group includes at least one lens, the fifth lens group has negative optical power, and the imaging magnification of the imaging lens is between 1.0 and 1.1 times.

[0007] Preferably, the imaging magnification of the imaging lens is between 1.0-1.05 times, more preferably between 1-1.03 times.

[0008] In another preferred example, the MicroLED display device is JBD 5000DPI AMuLED.

[0009] In another preferred embodiment, the imaging lens takes the aperture as an axis of symmetry.

[0010] In another preferred embodiment, the distance between the aperture and the output surface of the third lens group is between 20-30 mm, and / or the distance between the aperture and the input surface of the fourth lens group is between 20-30 mm.

[0011] In another preferred embodiment, the second lens group includes a second lens, the second lens is a plano-concave lens, and / or The spherical radius of the incident surface of the second lens is between -25 mm and -10 mm, and the spherical radius of the exit surface of the second lens is infinite, and / or The distance between the center of the incident surface of the second lens and the center of the exit surface of the second lens is between 1 mm and 5 mm, and the semi-aperture of the second lens is between 9 mm and 17 mm.

[0012] Preferably, the spherical radius of the incident surface of the second lens is between -20 mm and -15 mm, more preferably between -18 mm and -17 mm. Preferably, the distance between the center of the incident surface of the second lens and the center of the exit surface of the second lens is between 2 mm and 4 mm. Preferably, the semi-aperture of the second lens is between 11 mm and 16 mm.

[0013] In another preferred example, the third lens group includes a third lens, a fourth lens and a fifth lens, the third lens is a meniscus lens, the fourth lens is a plano-convex lens, and the fifth lens is a meniscus lens.

[0014] In another preferred embodiment, the air gap between each of the third lens, the fourth lens and the fifth lens is less than 0.5 mm.

[0015] Preferably, the air gap between each of the third lens, the fourth lens and the fifth lens is less than 0.2 mm.

[0016] In another preferred embodiment, the spherical radius of the incident surface of the third lens is between -60 mm and -40 mm, and the spherical radius of the exit surface of the third lens is between -30 mm and -20 mm; and / or The spherical radius of the incident surface of the fourth lens is infinite, and the spherical radius of the exit surface of the fourth lens is between -80 mm and -60 mm; and / or The spherical radius of the incident surface of the fifth lens is between 50 mm and 70 mm, and the spherical radius of the exit surface of the fifth lens is between 155 mm and 175 mm.

[0017] Preferably, the spherical radius of the exit surface of the fifth lens is between 160 mm and 170 mm.

[0018] In another preferred embodiment, the semi-diameters of the third lens, the fourth lens and the fifth lens are all between 11-22 mm, and / or The distance between the center of the incident surface and the center of the exit surface of the third lens, the distance between the center of the incident surface and the center of the exit surface of the fourth lens, and the distance between the center of the incident surface and the center of the exit surface of the fifth lens are all between 2mm and 6mm.

[0019] Preferably, the semi-diameter of the third lens is between 12-18 mm, and the semi-diameters of the fourth lens and the fifth lens are both between 15 mm and 20 mm.

[0020] Preferably, the distance between the center of the incident surface and the center of the exit surface of the third lens is slightly larger than the distance between the center of the incident surface and the center of the exit surface of the fourth lens and the fifth lens. Preferably, the distance between the center of the incident surface and the center of the exit surface of the third lens is between 3 mm and 6 mm, and the distance between the center of the incident surface and the center of the exit surface of the fourth lens and the distance between the center of the incident surface and the center of the exit surface of the fifth lens are both between 2 mm and 5 mm.

[0021] In another preferred example, the fourth lens group includes a sixth lens, a seventh lens and an eighth lens, the sixth lens is a meniscus lens, the seventh lens is a plano-convex lens, and the eighth lens is a meniscus lens.

[0022] In another preferred embodiment, the fifth lens and the sixth lens are both positive meniscus lenses, and are both curved toward the aperture stop.

[0023] In another preferred embodiment, the third lens and the eighth lens are both curved away from the aperture stop.

[0024] In another preferred embodiment, the spherical radius of the incident surface of the fifth lens is smaller than the spherical radius of the exit surface.

[0025] In another preferred embodiment, the fifth lens group includes a ninth lens, the ninth lens is a plano-concave lens, and / or The spherical radius of the incident surface of the ninth lens is infinite, and the spherical radius of the exit surface of the ninth lens is between -25 mm and -10 mm.

[0026] Preferably, the spherical radius of the exit surface of the ninth lens is between -20 mm and -15 mm, and more preferably, the spherical radius of the exit surface of the ninth lens is between -18 mm and -17 mm.

[0027] Preferably, the distance between the center of the incident surface of the ninth lens and the center of the exit surface of the ninth lens is between 1 mm and 5 mm, and the semi-aperture of the ninth lens is between 9 mm and 17 mm. More preferably, the distance between the center of the incident surface of the ninth lens and the center of the exit surface of the ninth lens is between 2 mm and 4 mm. Preferably, the semi-aperture of the ninth lens is between 11 mm and 16 mm.

[0028] In another preferred example, the imaging lens further includes a first lens group and a sixth lens group. In the light transmission direction, the first lens group is located in front of the second lens group, and the sixth lens group is located behind the fifth lens group; and / or the first lens group includes a first lens, which is a planar element, and / or the sixth lens group includes a tenth lens, which is a flat protective glass.

[0029] In another preferred example, the distance between the object plane end of the imaging lens and the first lens is between 15-25 mm, and / or the distance between the image plane end of the imaging lens and the tenth lens is between 15-25 mm.

[0030] In another preferred embodiment, the total length from the object plane end to the image plane end of the imaging lens is between 110 mm and 150 mm.

[0031] Preferably, the total length from the object plane end to the image plane end of the imaging lens is between 120 mm and 140 mm.

[0032] In another preferred example, the optogenetic stimulation device further includes a mechanical assembly structure, and the mechanical assembly structure is used to assemble the imaging lens.

[0033] In another preferred embodiment, the first lens group, the second lens group, the third lens group, the fourth lens group, the fifth lens group, and the sixth lens group are connected to the mechanical assembly structure via threads.

[0034] In another preferred example, the lens length of the imaging lens (ie, the length between the first lens group and the sixth lens group) is between 80-110 mm. Preferably, the lens length of the imaging lens is between 90-100 mm.

[0035] Preferably, the distance between the object plane end of the imaging lens and the first lens is between 18-22 mm. Preferably, the distance between the image plane end of the imaging lens and the tenth lens is between 17-22 mm.

[0036] In another preferred embodiment, the weight of the optogenetic stimulation device is between 120g and 160g, preferably, between 130g and 150g.

[0037] This application has at least one of the following advantages: (a) The head-mounted, high-precision optogenetic stimulation device for the non-human primate cerebral cortex of this application generates light stimulation patterns of arbitrary shapes and micron-level precision at any location in the cortex, meeting the needs of complex neural coding research; (b) The curvature of the imaging lens of the optogenetic stimulation device of the present application is designed so that the optical power is distributed more dispersedly rather than concentrated on one or two lenses, thereby achieving a smooth transition of the incident angle of light; (c) The optogenetic stimulation device of the present application can simplify the optical path design, reduce aberrations, and ensure the accuracy of stimulation power and positioning; (d) The optogenetic stimulation device of this application has a lightweight wearable structure (weighing only 139g), supports freely behaving animal experiments, and solves the pain points of traditional equipment that are bulky and have limited operation; (e) The optogenetic stimulation device of this application does not require complex fiber coupling and calibration processes, significantly improving experimental efficiency and reproducibility; (f) The optogenetic stimulation device of the present application significantly expands the application boundaries and capability limits of optogenetic stimulation technology by enabling free combination and precise control of stimulation position, shape, size, and intensity. It provides a powerful tool for in-depth exploration of the complex brain functions and neural circuit mechanisms of non-human primates, and plays an important role in promoting the development of optogenetics in the field of neuroscience research.

[0038] It should be understood that within the scope of the present invention, the above-mentioned technical features of the present invention and the technical features described in detail below (such as in the embodiments) can be combined with each other to form new or preferred technical solutions. Due to space limitations, they will not be listed here one by one. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. It should be understood that the drawings described below are merely some implementation examples of the present invention, and those skilled in the art can also derive other implementation examples based on these drawings without inventive effort.

[0040] Figure 1 1 is a schematic structural diagram of an imaging lens of a head-mounted high-precision optogenetic stimulation device for the cerebral cortex of non-human primates according to an embodiment of the present application; Figure 2 1 is a graph of field curvature and distortion of an imaging lens according to an embodiment of the present application. The vertical axis of the left graph represents the image field point. Figure 3 is a modulation transfer function (MTF) diagram of an imaging lens according to an embodiment of the present application; Figure 4 is a mechanical dimension diagram of an imaging lens according to an embodiment of the present application; Figure 5 This is an embodiment of a head-mounted, high-precision optogenetic stimulation device for the cerebral cortex of non-human primates based on Micro LEDs; Figure 6 This is a physical diagram of a head-mounted high-precision optogenetic stimulation device for the cerebral cortex of non-human primates according to one embodiment of the present application; Figure 7 is a schematic structural diagram of an imaging lens according to a comparative embodiment of the present application; Figure 8 1 is a graph of field curvature and distortion of an imaging lens according to a comparative embodiment of the present application. The vertical coordinate in the left graph is the image side field point. DETAILED DESCRIPTION

[0041] Through extensive and in-depth research, the inventors have developed for the first time a head-mounted, high-precision optogenetic stimulation device for the cerebral cortex of non-human primates. This device, through the setting of a MicroLED display device and an imaging lens, can generate light stimulation patterns with micron-level precision and arbitrary shapes at any location in the cortex, meeting the needs of complex neural coding research.

[0042] In the following description, many technical details are provided to help readers better understand this application. However, those skilled in the art will understand that even without these technical details and various changes and modifications based on the following embodiments, the technical solutions claimed in this application can be implemented.

[0043] the term As used herein, the term "radius R" refers to the radius of curvature R of each lens surface. A positive value for R indicates that the center of the sphere is to the right of the incident light (the surface is convex), and a negative value for R indicates that the center of the sphere is to the left of the incident light (the surface is concave). "Infinite" indicates that the surface is flat (the radius of curvature is infinite). That is, if the lens surface is convex toward the incident light, the radius of curvature R is positive, and if the lens surface is concave toward the incident light, the radius of curvature R is negative. As used herein, the term "thickness d" refers to the distance from the intersection of a current surface and the optical axis to the intersection of a next surface and the optical axis, or the distance from the center of a current surface to the center of a next surface (e.g., the distance between the center of the incident surface and the center of the exit surface of a lens); for example, in Table 1, the thickness 17.05 mm corresponding to the row of spherical surface number 0 means that the distance from the intersection of spherical surface 0 and the optical axis to the intersection of spherical surface 1 and the optical axis is 17.05 mm; the thickness 2 mm corresponding to the row of spherical surface number 1 means that the distance from the intersection of spherical surface 1 and the optical axis to the intersection of spherical surface 2 and the optical axis is 2 mm.

[0044] As used herein, the term "optical power" refers to a quantitative indicator of the optical performance of a lens, with the unit being diopters (D), which is equal to 1 / focal length (m). "Positive optical power" refers to a lens with positive optical power (e.g., a convex lens), which has a positive ability to converge light and a positive focal length. "Negative optical power" refers to a lens with negative optical power (e.g., a concave lens), which has a positive ability to diverge light and a negative focal length. In the present invention, all directional indications (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0045] A head-mounted high-precision optogenetic stimulation device for the cerebral cortex of non-human primates See also Figure 1, including a MicroLED display device and an imaging lens, the MicroLED is used to project various patterns, and the imaging lens is used to project the patterns from the MicroLED display device into images.

[0046] In one embodiment, the imaging lens is sequentially arranged from the object plane (Micro LED) to the image plane as a first lens group, a second lens group, a third lens group, an aperture, a fourth lens group, a fifth lens group, and a sixth lens group.

[0047] The third lens group and the fourth lens group are symmetrical about the aperture stop, and the second lens group and the fifth lens group are symmetrical about the aperture stop; The second lens group includes at least one lens, the second lens group has negative optical power, the third lens group includes a plurality of lenses, the third lens group has positive optical power and is used to converge light diverging through the second lens group into quasi-parallel light and correct spherical aberration; The fourth lens group includes multiple lenses, the fourth lens group has positive optical power, the fourth lens group and the third lens group are optically conjugate, the fifth lens group includes at least one lens, the fifth lens group has negative optical power, and the imaging magnification of the imaging lens is between 1.0 and 1.1 times.

[0048] In one embodiment, the first lens group includes a first lens L1.

[0049] Preferably, the second lens group includes a second lens L2. L2 is a plano-concave lens. Preferably, the spherical radius of the incident surface of the second lens L2 is between -25 mm and -10 mm, and the spherical radius of the exit surface of the second lens L2 is infinite.

[0050] Preferably, the distance between the center of the incident surface of the second lens L2 and the center of the exit surface of the second lens L2 is between 1 mm and 5 mm, and the semi-aperture of the second lens L2 is between 9 mm and 17 mm.

[0051] Preferably, the spherical radius of the incident surface of the second lens L2 is between -20 mm and -15 mm, more preferably between -18 mm and -17 mm. Preferably, the distance between the center of the incident surface of the second lens L2 and the center of the exit surface of the second lens is between 2 mm and 4 mm. Preferably, the semi-aperture diameter of the second lens L2 is between 11 mm and 16 mm.

[0052] In one embodiment, the third lens group includes, in order along the light beam direction, a third lens L3, a fourth lens L4, and a fifth lens L5. Lens L3 is a meniscus lens, lens L4 is a plano-convex lens, and lens L5 is a meniscus lens. The curvatures of lens L3, lens L4, and lens L5 are designed to distribute the optical power more dispersedly, rather than concentrating it on just one or two lenses. This results in a smooth transition in the angle of incidence of the light. Preferably, the air gaps between lens L3, lens L4, and lens L5 are less than 0.5 mm.

[0053] Preferably, the spherical radius of the incident surface of the third lens L3 is between -60 mm and -40 mm, and the spherical radius of the exit surface of the third lens L3 is between -30 mm and -20 mm.

[0054] Preferably, the spherical radius of the incident surface of the fourth lens L4 is infinite, and the spherical radius of the exit surface of the fourth lens L4 is between -80 mm and -60 mm.

[0055] Preferably, the spherical radius of the incident surface of the fifth lens L5 is between 50 mm and 70 mm, and the spherical radius of the exit surface of the fifth lens L5 is between 155 mm and 175 mm.

[0056] Preferably, the semi-apertures of the third lens L3, the fourth lens L4, and the fifth lens L5 are all between 11 and 22 mm. Preferably, the distance between the center of the incident surface and the center of the exit surface of the third lens L3, the distance between the center of the incident surface and the center of the exit surface of the fourth lens L4, and the distance between the center of the incident surface and the center of the exit surface of the fifth lens L5 are all between 2 and 6 mm.

[0057] In one embodiment, the second lens L2 is made of a material with a low refractive index and a high Abbe number. The third lens L3, the fourth lens L4, and the fifth lens L5 are made of materials with a high refractive index and a low Abbe number. This combination can achieve an achromatic effect.

[0058] Preferably, the fourth lens group includes a sixth lens L6, a seventh lens L7, and an eighth lens L8 in sequence along the light beam direction. L6 is a meniscus lens, L7 is a plano-convex lens, and L8 is a meniscus lens.

[0059] Preferably, the fifth lens group includes a ninth lens L9, which is a plano-concave lens.

[0060] Preferably, the sixth lens group includes a tenth lens L10.

[0061] In order to make the purpose, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings. It should be understood that these are only some examples that the present invention may take, but are not intended to limit the scope of the present invention.

[0062] Example 1 See also Figure 1-Figure 5 The present application provides an optical device for stimulating the cerebral cortex of non-human primates based on optogenetic technology, the optical device comprising two parts: MicroLEDs are used to project various patterns. JBD 5000DPI AMuLEDs are used, with a resolution of 1280*720, a chip size of 8.4*5.93mm, and a lighting power consumption of 0.1-5W.

[0063] The imaging lens is used to project the pattern from the MicroLED display device. The imaging design of this lens adopts a fully symmetrical structure, with a total length of 128.95mm (object to image) and a lens length of 94.11mm. The lens includes a first lens group, a second lens group, a third lens group, an aperture, a fourth lens group, a fifth lens group, and a sixth lens group, which are arranged in sequence along the light transmission direction from the object plane (MicroLED) to the image plane. All lenses are arranged coaxially, and the imaging magnification of this lens is 1.029 times. This imaging lens is suitable for the visible light band. In optogenetics, calcium imaging, or other imaging experiments requiring special or single wavelengths, it is only necessary to add specific wavelength filters to the structure.

[0064] See also Figure 1 , Figure 1 This is a schematic diagram of the structure of an imaging lens according to an embodiment of the present invention. The first lens group is a planar element, corresponding to lens L1. This lens group is used for mechanical protection.

[0065] The second lens group corresponds to lens L2 and is a plano-concave lens that can achieve strong negative focal length within a short working distance. The light passing through the first and second lens groups is divergent. The beneficial effect of this technical solution is that it can increase the field of view.

[0066] The third lens group, corresponding to lenses L3-L5, has positive focal power, converging diverging light into parallel light while also correcting spherical aberration. The beneficial effect of this technical solution lies in the high-refractive-index material used in the third lens group, which achieves both strong focal power, shortened optical paths, and achromatic aberration. The air gap between the three lenses is less than 0.2mm, minimizing the angle of incidence of light entering lenses L4 and L5, thus avoiding the introduction of further aberrations. The curvature of meniscus lens L5 toward the aperture helps reduce aberrations in oblique light rays.

[0067] The aperture corresponds to S1. The position of the aperture has a great influence on coma and astigmatism. This design uses the aperture as the axis of symmetry to achieve a significant reduction in aberrations.

[0068] The fourth lens group, corresponding to lenses L6-L8, has positive optical power and forms an optical conjugate with the third lens group. This technical solution has the beneficial effect of focusing the light beam while simultaneously offsetting residual aberrations. Meniscus lens L8 is curved toward the aperture, further suppressing marginal ray aberrations.

[0069] The fifth lens group corresponds to lens L9, which is a plano-concave lens that corresponds to lens L2 and has negative optical power. The beneficial effect of this technical solution is to compensate for the residual aberration of the front group.

[0070] The sixth lens group corresponds to lens L10, which is a flat protective glass with a refractive index of 1.528 to match the image-side refractive index transition.

[0071] An aperture is provided between the third lens group and the fourth lens group. The third lens group and the fourth lens group are symmetrical about the aperture. The second lens group and the fifth lens group are symmetrical about the aperture.

[0072] The lenses in this optical design are all spherical lenses, which helps to reduce processing difficulty and thus reduce manufacturing costs. See Table 1, which provides an example of the parameters of each lens.

[0073] Table 1. Parameters of each lens in the lens group

[0074] The disclosed embodiments also provide a mechanical assembly structure for assembling the aforementioned imaging lens. The structure is made of LY6061 (aluminum alloy). The mechanical assembly structure utilizes threaded connections between the object and image plane ends and each lens assembly, allowing for flexible attachment of optical components or mechanical sleeves. For example, an imaging lens filter can be installed within a mechanical sleeve, which is then screwed onto the mechanical assembly structure.

[0075] The imaging lens provided in the embodiment of the present application can project the pattern projected by the MicroLED onto the cerebral cortex of non-human primates with a magnification of nearly 1x.

[0076] Figure 2 The following graphs show the field curvature and distortion of the imaging lens of an embodiment of the present invention. The left graph shows the image-side field of view points on the ordinate. The abscissa represents the defocus at each point, with defocus values ​​of 0.059mm and 0.085mm for the tangential and sagittal planes, respectively. The right graph shows the distortion value on the abscissa, which is less than 0.01% across the entire field of view and nearly zero at the edges. Excellent control of field curvature and distortion ensures topologically faithful mapping of the MicroLED image source to the visual cortex.

[0077] Figure 3Table 2 shows the modulation transfer function (MTF) of the imaging lens according to an embodiment of the present invention. The MTF for the on-axis field of view (blue curve) at 40 lp / mm is 70%, while the MTF for the off-axis field of view (curves other than the blue curve) at 40 lp / mm is 60%-80%. When the MTF is 20%, the line pair count is 104, indicating a lens resolution of approximately 4.8μm. The solid line represents the meridional plane (the plane defined by the off-axis object point and the optical axis), and the dashed line represents the sagittal plane (the plane containing the object point and perpendicular to the meridional plane).

[0078] Table 2. MTF values ​​of imaging lenses

[0079] This application also provides Figure 4 、 Figure 5 as well as Figure 6 ,in Figure 4 The mechanical dimensions of Example 1 of the present invention are shown in FIG. 1 , where the total length of the lens is 91.72 mm and the diameter is 39.2 mm.

[0080] Figure 5 The embodiment 1 of the present invention is shown as a Micro LED-based implementation, demonstrating that a pattern projected by the Micro LED is projected onto the cerebral cortex through a Micro LED device and an imaging lens.

[0081] Figure 6 A photo of an optical device for stimulating the cerebral cortex of non-human primates based on optogenetics is given. The black part is the imaging lens, and the gold part is the data transmission line of the MicroLED, one end of which is attached to the center of the cover and screwed onto the lens through a thread. Figure 1 The other end is connected to the MicroLED control circuit board.

[0082] Example 2 See also Figure 7 and Figure 8 , this embodiment 2 is a pair of ratios, wherein in this embodiment 2, a comparative imaging lens is provided, which includes a first lens group, a second lens group, a third lens group, an aperture, a fourth lens group, a fifth lens group, and a sixth lens group in sequence along the light transmission direction. Similar to embodiment 1, the third lens group and the fourth lens group are symmetrical about the aperture, and the second lens group and the fifth lens group are symmetrical about the aperture. In this embodiment 2, different from the first embodiment, the third and fourth lens groups of the comparative imaging lens are both composed of two lenses and the lens parameters are different.

[0083] Referring to Table 3, the number and parameters of lenses in the first lens group, the second lens group, the third lens group, the fourth lens group, the fifth lens group, and the sixth lens group are provided, wherein the first lens group includes a first comparison lens M1, the second lens group includes a second comparison lens M2, the third lens group includes a third comparison lens M3 and a fourth comparison lens M4, the fourth lens group includes a fifth comparison lens M5 and a sixth comparison lens M6, the fifth lens group includes a seventh comparison lens M7, and the eighth lens group includes an eighth comparison lens M8.

[0084] See Table 4 and Figure 8 Table 4 shows the MTF values ​​of the comparative imaging lens. It can be seen from the MTF values ​​that the effect of the comparative imaging lens is far worse than that of the imaging lens provided in Example 1.

[0085] Table 3. Parameters of each comparison lens in the lens group

[0086] Table 4. Corresponding MTF values

[0087] It should be noted that in the present application documents, relational terms such as first and second are used solely to distinguish one entity or operation from another, and do not necessarily require or imply any actual relationship or order between these entities or operations. Furthermore, the terms "comprise," "include," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or device comprising a series of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. Without further limitation, an element defined by the phrase "comprising a" does not preclude the presence of other identical elements in the process, method, article, or device comprising the element. In the present application documents, reference to performing an action according to an element means performing the action at least according to that element, including two situations: performing the action only according to that element, and performing the action according to that element and other elements. Expressions such as "multiple," "multiple," and "multiple" include "two," "twice," "two kinds," and "more than two," "more than two times," and "more than two kinds."

[0088] All documents mentioned in this application are considered to be included in their entirety in the disclosure of this application so that they can be used as a basis for modification when necessary. In addition, it should be understood that after reading the above disclosure of this application, those skilled in the art may make various changes or modifications to this application, and these equivalent forms also fall within the scope of protection claimed in this application.

Claims

1. A head-mounted high-precision optogenetic stimulation device for the cerebral cortex of non-human primates, characterized in that: include: a MicroLED display device configured to provide image light and project a stimulation pattern; An imaging lens configured to image the stimulation pattern output by the MicroLED display device onto the cerebral cortex of the non-human primate, wherein the imaging lens is a fully symmetrical structure and includes, in sequence along the light transmission direction, a second lens group, a third lens group, an aperture, a fourth lens group, and a fifth lens group. The third lens group and the fourth lens group are symmetrical about the aperture stop, and the second lens group and the fifth lens group are symmetrical about the aperture stop; The second lens group includes at least one lens, the second lens group has negative optical power, the third lens group includes a plurality of lenses, the third lens group has positive optical power and is used to converge light diverging through the second lens group into quasi-parallel light and correct spherical aberration; The fourth lens group includes multiple lenses, the fourth lens group has positive optical power, the fourth lens group and the third lens group are optically conjugate, the fifth lens group includes at least one lens, the fifth lens group has negative optical power, and the imaging magnification of the imaging lens is between 1.0 and 1.1 times.

2. The optogenetic stimulation device according to claim 1, wherein The second lens group includes a second lens, the second lens is a plano-concave lens, and / or The spherical radius of the incident surface of the second lens is between -25 mm and -10 mm, and the spherical radius of the exit surface of the second lens is infinite, and / or The distance between the center of the incident surface of the second lens and the center of the exit surface of the second lens is between 1 mm and 5 mm, and the semi-aperture of the second lens is between 9 mm and 17 mm.

3. The optogenetic stimulation device according to claim 1, wherein The third lens group includes a third lens, a fourth lens and a fifth lens, the third lens is a meniscus lens, the fourth lens is a plano-convex lens, and the fifth lens is a meniscus lens.

4. The optogenetic stimulation device according to claim 3, wherein The air gap between each of the third lens, the fourth lens and the fifth lens is less than 0.5 mm.

5. The optogenetic stimulation device according to claim 4, wherein The spherical radius of the incident surface of the third lens is between -60 mm and -40 mm, and the spherical radius of the exit surface of the third lens is between -30 mm and -20 mm; and / or The spherical radius of the incident surface of the fourth lens is infinite, and the spherical radius of the exit surface of the fourth lens is between -80 mm and -60 mm; and / or The spherical radius of the incident surface of the fifth lens is between 50 mm and 70 mm, and the spherical radius of the exit surface of the fifth lens is between 155 mm and 175 mm.

6. The optogenetic stimulation device according to claim 5, wherein The semi-aperture diameters of the third lens, the fourth lens, and the fifth lens are all between 11 and 22 mm, and / or The distance between the center of the incident surface and the center of the exit surface of the third lens, the distance between the center of the incident surface and the center of the exit surface of the fourth lens, and the distance between the center of the incident surface and the center of the exit surface of the fifth lens are all between 2mm and 6mm.

7. The optogenetic stimulation device according to claim 5, wherein The fourth lens group includes a sixth lens, a seventh lens and an eighth lens, the sixth lens is a meniscus lens, the seventh lens is a plano-convex lens, and the eighth lens is a meniscus lens.

8. The optogenetic stimulation device according to claim 7, wherein The fifth lens group includes a ninth lens, the ninth lens is a plano-concave lens, and / or The spherical radius of the incident surface of the ninth lens is infinite, and the spherical radius of the exit surface of the ninth lens is between -25 mm and -10 mm.

9. The optogenetic stimulation device according to any one of claims 1 to 8, wherein: The imaging lens further includes a first lens group and a sixth lens group. In the light transmission direction, the first lens group is located in front of the second lens group, and the sixth lens group is located behind the fifth lens group. The first lens group includes a first lens, which is a planar element. The sixth lens group includes a tenth lens, which is a flat protective glass.

10. The optogenetic stimulation device according to claim 9, wherein The distance between the object plane end of the imaging lens and the first lens is between 15-25 mm, and / or the distance between the image plane end of the imaging lens and the tenth lens is between 15-25 mm.

11. The optogenetic stimulation device according to any one of claims 1 to 8, wherein: The total length from the object plane end to the image plane end of the imaging lens is between 110 mm and 150 mm.

Images