Method and model for determining risk level of pig xenogenic organ transplantation pathogen and application

Abstract

The invention relates to the technical field of computers, in particular to a method and a model for determining the risk level of pig xenogeny organ transplantation pathogens and application. By comprehensively collecting the types of pig pathogens and the characteristics of each pathogen, a risk grade evaluation model of various pig pathogens in xenogeny organ transplantation is established, and a scientific basis is provided for precise infectious disease prevention and control of donor pigs. The objective of the invention is to solve the problem of how to evaluate the risk level of pig xenogeny organ transplantation related pathogens.

Description

Technical Field

[0001] The present application relates to the field of computer technology, and in particular to a method, model and application for determining the risk level of pig xenotransplant pathogens. Background Art

[0002] With the clinical progress of pig-to-human xenotransplantation, pig-derived organs are expected to solve the global organ shortage problem. However, the transplantation of xenogeneic organs needs to consider the risk of infection of the transplant recipient caused by pathogens carried by the original host. For example, pig-derived pathogens such as viruses, bacteria, fungi and parasites can easily cause infection of immunosuppressed transplant recipients.

[0003] Current research lacks a comprehensive and systematic approach to assessing the pathogen risk of porcine xenotransplantation. A common approach considers the risk of pathogens in xenotransplantation primarily from the perspectives of their epidemiology and pathogenicity. However, this approach has significant drawbacks: Firstly, the assessment results obtained by this approach are subject to subjective influences, lacking objectivity and reproducibility; secondly, pathogen risk assessment for porcine xenotransplantation is highly specific, and assessing pathogen risk solely from the perspectives of pathogenicity and / or epidemiology cannot systematically reflect the risk of porcine pathogens in xenotransplantation.

[0004] Therefore, a comprehensive and systematic approach to assess the risk level of pathogens associated with porcine xenotransplantation is needed to provide an objective basis for the whole-process production of biosafe donor pigs. Summary of the Invention

[0005] The main purpose of this application is to provide a method for determining the risk level of pig xenotransplant pathogens, aiming to solve the problem of how to evaluate the risk level of pig xenotransplant-related pathogens.

[0006] To achieve the above objectives, the present application provides a method for determining the risk level of pig xenotransplant pathogens, the method comprising:

[0007] Determine the pathogen type of the pathogen data collected from pig xenotransplanted organs;

[0008] If the pathogen type is the first pathogen type, determining the pathogen risk level of the pathogen data to be tested according to the evaluation score of the pathogen data in the first pathogen index;

[0009] If the pathogen type is the second pathogen type, determining the pathogen risk level of the pathogen data to be tested according to the evaluation score of the pathogen data in the second pathogen index;

[0010] The first pathogen index includes H-index value, infection frequency, pathogenicity, mortality rate, number of countries where the pathogen is distributed, whether it is in an emerging state, transmission mode, whether pigs are the main host, infection status and whether it has been reported by the World Health Organization;

[0011] The second pathogenic indicator includes the first pathogenic indicator, organ transplant relevance, whether it is in a zoonotic state, and the number of cases of pathogen transmission from pigs to humans.

[0012] Optionally, the step of determining the pathogen risk level of the pathogen data to be tested of the first pathogen type or the second pathogen type includes:

[0013] When the sum of the evaluation scores of the first pathogen index / the second pathogen index of the pathogen data is within a first numerical range, determining the pathogen risk level to be the first risk level;

[0014] When the sum of the evaluation scores of the first pathogen index / the second pathogen index of the pathogen data is within a second numerical range, determining the pathogen risk level to be the second risk level;

[0015] When the sum of the evaluation scores of the first pathogen index / the second pathogen index of the pathogen data is within a third numerical range, determining the pathogen risk level to be the third risk level;

[0016] The first numerical interval is larger than the second numerical interval and larger than the third numerical interval.

[0017] Optionally, the first risk level indicates that the pathogen data to be tested is a high-risk pathogen that requires routine monitoring, the second risk level indicates that the pathogen data to be tested is a medium- or high-risk pathogen that requires dynamic monitoring based on epidemiology, and the third risk level indicates that the pathogen data to be tested is a low-risk pathogen that does not require monitoring.

[0018] In addition, to achieve the above objectives, the present application also provides a risk assessment model for porcine xenotransplantation pathogens, the risk assessment model for porcine xenotransplantation pathogens comprising:

[0019] The first pathogen index used to score the pathogen data of the first pathogen type, including H-index value, infection frequency, pathogenicity, mortality rate, number of countries where the pathogen is distributed, whether it is in an emerging state, transmission mode, whether pigs are the main host, infection status, and whether it has been reported by the World Health Organization;

[0020] The second pathogen index used to score the pathogen data to be tested of the second pathogen type includes the first pathogen index, organ transplant relevance, whether it is in a zoonotic state, and the number of cases of pathogen transmission from pigs to humans.

[0021] Optionally, the first pathogenicity indicator and the second pathogenicity indicator include at least two evaluation levels, and the first pathogenicity indicator or the second pathogenicity indicator associated with the pathogenicity data to be detected has different corresponding weight scores when at different evaluation levels.

[0022] In addition, to achieve the above-mentioned purpose, the present application also provides a first pathogen type scoring table and a second pathogen type scoring table obtained by using the risk level determination method of porcine xenotransplant pathogens and / or the risk assessment model of porcine xenotransplant pathogens.

[0023] In addition, to achieve the above-mentioned purpose, the present application also provides a non-zoonotic porcine xenotransplantation pathogen risk level classification table obtained by using the risk level determination method of porcine xenotransplantation pathogens and / or the risk assessment model of porcine xenotransplantation pathogens.

[0024] In addition, to achieve the above-mentioned purpose, the present application also provides a risk level classification table for zoonotic porcine xenotransplant pathogens obtained by using the risk level determination method for porcine xenotransplant pathogens and / or the risk assessment model for porcine xenotransplant pathogens.

[0025] In addition, to achieve the above-mentioned purpose, the present application also provides a method for determining the risk level of a porcine xenotransplant pathogen and / or the application of the risk assessment model for a porcine xenotransplant pathogen in monitoring the pathogen infection status of a donor pig before xenotransplantation.

[0026] In addition, to achieve the above-mentioned purpose, the present application also provides a computer system for evaluating the risk level of pig xenotransplant pathogens. The computer system for evaluating the risk level of pig xenotransplant pathogens includes: a memory, a processor, and a computer program stored on the memory and executable on the processor. When the computer program is executed by the processor, the steps of the method for determining the risk level of pig xenotransplant pathogens as described in any one of the above items are implemented.

[0027] This application has at least the following beneficial effects:

[0028] 1. By comprehensively collecting the types and characteristics of porcine pathogens, and establishing a risk level assessment model for various porcine pathogens in xenotransplantation, we provide a scientific basis for the precise prevention and control of infectious diseases in donor pigs;

[0029] 2. Based on the risk level evaluation rules for pig xenotransplant pathogens proposed in this application, the risk level of pig pathogens was evaluated, and a risk level classification table for non-zoonotic and zoonotic pathogens for xenotransplantation was obtained, providing a scientific basis for the precise prevention and control of infectious diseases in donor pigs;

[0030] 3. Based on the risk level evaluation rules for pig xenotransplant pathogens proposed in this application, monitor the pathogen infection status of donor pigs before xenotransplantation to provide reference information for the production of biosafe donor pigs for transplanted organs. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a flow chart of the first embodiment of the method for determining the risk level of a pig xenotransplant pathogen of the present application;

[0032] Figure 2 This is a schematic diagram of the architecture of the hardware operating environment of a computer system for pathogen risk level assessment of porcine xenotransplantation according to an embodiment of the present application.

[0033] The realization of the objectives, functional features and advantages of this application will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0034] To better understand the above technical solutions, exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although exemplary embodiments of the present disclosure are shown in the accompanying drawings, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments described herein. Rather, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the present disclosure to those skilled in the art.

[0035] First embodiment

[0036] Reference Figure 1 This embodiment provides a method for determining the risk level of a pig xenotransplant pathogen, the method comprising the following steps:

[0037] S10, determining the pathogen type of the pathogen data to be tested collected from the pig xenotransplanted organ;

[0038] In this embodiment, the pathogens of pig xenotransplanted organs are divided into two categories, namely the first pathogen type and the second pathogen type.

[0039] The first type of pathogen is characterized as a non-zoonotic pathogen, which refers to a pathogen that only infects humans or specific animals (such as the donor pig in this embodiment) and does not spread naturally between humans and animals.

[0040] The second type of pathogen is characterized by zoonotic pathogens, which refer to pathogens that can be naturally transmitted between humans and animals. Such pathogens can cause zoonoses, posing a dual threat to human and animal health.

[0041] S20, if the pathogen type is the first pathogen type, determining the pathogen risk level of the pathogen data to be tested based on the evaluation score of the pathogen data in the first pathogen index;

[0042] S30, if the pathogen type is the second pathogen type, determining the pathogen risk level of the pathogen data to be tested according to the evaluation score of the pathogen data in the second pathogen index;

[0043] In this embodiment, different evaluation indicators are preset for the first pathogen type and the second pathogen type, respectively, wherein the score of each evaluation indicator is an input value rather than a calculated value.

[0044] In order to accurately reflect the risk level of the pathogen data to be tested of the first pathogen type characterized as a non-zoonotic pathogen, in this embodiment, the relevant characteristics of the swine pathogens are summarized from the mainstream databases (Web of Science, PubMed, Google Scholar and ScienceDirect) and the public swine pathogen databases (CFSPH, WOAH, SHIC, MSD). The data include the epidemic characteristics, pathogenicity, transmission route, cell / tissue / organ specificity, zoonotic potential, vertical transmission ability, new / recurring status, H-index index, and cases of pathogen transmission in human and pig xenotransplantation. In addition to the epidemic characteristics (in the Web of Science database, the number of studies on the pathogen in various countries is queried to indirectly reflect the epidemic distribution characteristics of the pathogen in the world) and H-index (queried from the Web of Science database), the remaining pathogen characteristics are queried from relevant literature and databases. After a large number of experiments and verifications, 10 first pathogen indicators and 13 second pathogen indicators were integrated.

[0045] Specifically, the first pathogen indicator includes the H-index value, infection frequency, pathogenicity, mortality rate, number of countries where the pathogen is distributed, whether it is an emerging disease, transmission mode, whether pigs are the primary host, infection status, and whether it has been reported by the World Health Organization. The following is an explanation of the definition of each indicator and its correlation with the pathogen risk level:

[0046] The H-index value refers to the value queried from the Web of Science database and is used to evaluate the academic influence of a certain research or scholar and to reflect the degree of harm caused by pathogens. The H-index value is positively correlated with the pathogen risk level.

[0047] Infection frequency refers to a quantitative value reflecting the infection level of a pathogen. It is used to evaluate whether the pathogen frequently infects domestic pigs and assess its potential persistent harm. The infection frequency is positively correlated with the pathogen risk level.

[0048] Pathogenicity is used to reflect the degree of damage caused by the pathogen to the organs of domestic pigs, including but not limited to xenotransplanted heart, liver, lungs or kidneys. Pathogenicity is positively correlated with the pathogen risk level.

[0049] The mortality rate is used to reflect the severity of an infectious disease and to evaluate the degree of harm the pathogen poses to the health of domestic pigs. The pathogenicity is positively correlated with the pathogen risk level.

[0050] The number of countries where the pathogen is distributed is to query the number of reported cases of the pathogen in each country, which indirectly reflects the distribution characteristics of the pathogen. Among them, if the number of studies reporting a pathogen in countries on three different continents exceeds 6, it is defined as a global epidemic, otherwise it is a regional epidemic. The number of countries where the pathogen is distributed is positively correlated with the pathogen risk level.

[0051] Whether it is in an emerging state refers to whether the pathogen has broken out for the first time and caused serious harm. This indicator directly reflects the importance of the pathogen. If it is, it means that the pathogen risk level of the pathogen to be tested is high.

[0052] The transmission mode is divided into vertical transmission through the mother-offspring pathway and / or horizontal transmission through the external environment pathway, reflecting the complexity of pathogen transmission. If the transmission mode includes both vertical transmission and horizontal transmission, it means that the pathogen risk level of the pathogen to be tested is high.

[0053] Whether pigs are the main host is used to assess the association between the pathogen and the harm to pigs. If so, it means that the pathogen risk level of the pathogen to be tested is high.

[0054] The infection status refers to whether the pathogen infects pigs in a natural state or only infects pigs under experimental conditions, reflecting the importance of the pathogen to pigs. Among them, if the pathogen infects pigs in a natural state, it means that the pathogen risk level of the pathogen to be tested is higher.

[0055] Whether it has been reported by the World Health Organization can also reflect the importance of the pathogen in the pig farming process. If it has been reported, it means that the pathogen risk level of the pathogen to be tested is higher.

[0056] Specifically, the second pathogen indicator includes the 10 indicators of the aforementioned first pathogen indicator, plus 3 indicators: organ transplant relevance, whether it is in a zoonotic state, and the number of cases of pathogen transmission from pigs to humans, totaling 13 indicators.

[0057] The relevance of organ transplantation is positively correlated with the number of cases of pathogen transmission in pig-to-non-human primate, pig-to-human and human-to-human organ transplantation. Among them, if there is a reported case of pig-to-human transmission, it means that the pathogen risk level of the pathogen to be tested is higher. If it is from pig to non-human primate, the risk level is second, and if it is from human to human, the risk level is third. If there is no reported case of infection in organ transplantation, it means the risk level is the lowest.

[0058] Whether it is in a zoonotic state refers to whether the pathogen can infect humans and pigs at the same time. If so, it is in a zoonotic state, which means the risk level is higher.

[0059] The number of cases of pathogen transmission from pigs to humans refers to the number of reported cases of transmission from pigs to humans for the pathogen that can be queried, and this number is positively correlated with the pathogen risk level.

[0060] In this embodiment, after setting the weight score for each indicator based on the above-mentioned ideas, the scores are scored manually or by machine and then input. The total score obtained by adding up these scores can be used to determine the pathogen risk level of the pathogen data to be tested based on the size of the total score.

[0061] In the technical solution provided in this embodiment, the pathogens to be tested for pig xenotransplantation are divided into a first pathogen type characterized as non-zoonotic and a second pathogen type characterized as zoonotic. According to the pathogen type corresponding to the pathogen to be tested, the pathogen index corresponding to the type is selected for scoring. The obtained evaluation scores are weighted and summed to determine the pathogen risk level of the pathogen data to be tested. The specific definition of each indicator and its correlation with the pathogen risk level are provided. Comprehensive pathogen characteristics are collected and an assessment of the risk level of various pig pathogens in xenotransplantation is established, providing a scientific basis for the precise prevention and control of infectious diseases in donor pigs.

[0062] Second embodiment

[0063] Based on the first embodiment, in this embodiment, the step of determining the pathogen risk level of the pathogen data to be tested of the first pathogen type or the second pathogen type includes:

[0064] S100, when the sum of the evaluation scores of the first pathogen index / the second pathogen index of the pathogen data is within a first numerical range, determining the pathogen risk level as a first risk level;

[0065] S200, when the sum of the evaluation scores of the first pathogen index / the second pathogen index for the pathogen data is within a second numerical range, determining the pathogen risk level to be a second risk level;

[0066] S300: When the sum of the evaluation scores of the first pathogen index / the second pathogen index for the pathogen data is within a third numerical range, determining the pathogen risk level as a third risk level;

[0067] The first numerical interval is larger than the second numerical interval and larger than the third numerical interval.

[0068] In this embodiment, whether it is the first pathogen indicator or the second pathogen indicator, a hierarchical scoring method is used to comprehensively consider the scores of each indicator to calculate the total risk score of the pathogen (out of 100 points). According to the total score, the pathogen is divided into three risk levels: the first risk level, the second risk level and the third risk level.

[0069] Specifically and optionally, the first risk level characterizes that the pathogen data to be tested is a high-risk pathogen (priority risk pathogen) that requires routine monitoring, the second risk level characterizes that the pathogen data to be tested is a medium- to high-risk pathogen (that may evolve into a high-risk pathogen) that requires dynamic monitoring based on epidemiology, and the third risk level characterizes that the pathogen data to be tested is a low-risk pathogen that does not require monitoring.

[0070] For example, in some specific embodiments, the first numerical interval is 80-100 points, the second numerical interval is 60-79 points, and the low-risk pathogen is less than 60 points.

[0071] In addition, this example also provides specific descriptions of pathogens at different risk levels:

[0072] Priority risk pathogens are ranked according to the significance of indicators such as pathogenicity, transmissibility and relevance to organ transplantation; medium- and high-risk pathogens are characterized by having two or more significant characteristics, such as widespread prevalence and high mortality, and emphasize that the epidemiological characteristics of pig pathogens vary depending on the region; low-risk pathogens are characterized by species that are not commonly infecting pigs or associated with important clinical syndromes.

[0073] In addition, as an implementation solution, the present invention provides a risk assessment model for pig xenotransplant pathogens, which includes:

[0074] The first pathogen index used to score the pathogen data of the first pathogen type, including H-index value, infection frequency, pathogenicity, mortality rate, number of countries where the pathogen is distributed, whether it is in an emerging state, transmission mode, whether pigs are the main host, infection status, and whether it has been reported by the World Health Organization;

[0075] The second pathogen index used to score the pathogen data to be tested of the second pathogen type includes the first pathogen index, organ transplant relevance, whether it is in a zoonotic state, and the number of cases of pathogen transmission from pigs to humans.

[0076] Further and optionally, the first pathogenicity indicator and the second pathogenicity indicator include at least two evaluation levels, and the first pathogenicity indicator or the second pathogenicity indicator associated with the pathogenicity data to be measured has different corresponding weight scores when at different evaluation levels.

[0077] In addition, as an implementation solution, the present application provides a first pathogen type scoring table and a second pathogen type scoring table obtained by using the aforementioned method for determining the risk level of porcine xenotransplant pathogens and / or the aforementioned risk assessment model for porcine xenotransplant pathogens:

[0078] Table 1. First pathogen type scoring table

[0079]

[0080] Table 2. Second pathogen type scoring table

[0081]

[0082]

[0083] In addition, as an implementation scheme, the embodiment of the present application provides a non-zoonotic porcine xenotransplant pathogen risk level classification table obtained by using the aforementioned porcine xenotransplant pathogen risk level determination method and / or the aforementioned porcine xenotransplant pathogen risk assessment model, the non-zoonotic porcine xenotransplant pathogen risk level classification table including:

[0084] Table 3. Risk classification of pathogens in non-zoonotic porcine xenotransplantation

[0085]

[0086]

[0087]

[0088]

[0089]

[0090] In addition, as an implementation scheme, the embodiment of the present application provides a risk level classification table for zoonotic porcine xenotransplant pathogens obtained by using the aforementioned risk level determination method for porcine xenotransplant pathogens and / or the aforementioned risk assessment model for porcine xenotransplant pathogens. The risk level classification table for zoonotic porcine xenotransplant pathogens includes:

[0091] Table 4. Risk classification of pathogens in zoonotic pig xenotransplantation

[0092]

[0093]

[0094]

[0095]

[0096]

[0097]

[0098] In addition, as an implementation scheme, an embodiment of the present application provides a method for determining the risk level of a porcine xenotransplant pathogen as described above and / or an application of a risk assessment model for a porcine xenotransplant pathogen as described above in monitoring the pathogen infection status of a donor pig before xenotransplantation.

[0099] Specifically and optionally, the application specifically includes a recommended table of porcine pathogens detected using metagenomic technology:

[0100] Table 5. Recommended list of pig pathogens detected using metagenomic technology

[0101]

[0102]

[0103]

[0104]

[0105] In addition, as an implementation solution, Figure 2 This is a schematic diagram of the architecture of the hardware operating environment of a computer system for pathogen risk level assessment of porcine xenotransplantation according to an embodiment of the present application.

[0106] like Figure 2 As shown, the computer system for evaluating the pathogen risk level of pig xenotransplantation may include: a processor 1001, such as a CPU, a memory 1005, a user interface 1003, a network interface 1004, and a communication bus 1002. Among them, the communication bus 1002 is used to realize the connection and communication between these components. The user interface 1003 may include a display screen (Display), an input unit such as a keyboard (Keyboard), and the user interface 1003 may also include a standard wired interface and a wireless interface. The network interface 1004 may optionally include a standard wired interface and a wireless interface (such as a WI-FI interface). The memory 1005 may be a high-speed RAM memory or a stable memory (non-volatile memory), such as a disk memory. The memory 1005 may also be a storage device independent of the aforementioned processor 1001.

[0107] Those skilled in the art will understand that Figure 2The computer system architecture for evaluating the pathogen risk level of porcine xenotransplantation shown in the does not constitute a limitation on the computer system for evaluating the pathogen risk level of porcine xenotransplantation, and may include more or fewer components than shown in the figure, or combine certain components, or arrange the components differently.

[0108] like Figure 2 As shown, the memory 1005 as a storage medium may include an operating system, a network communication module, a user interface module, and a computer program. The operating system is a program that manages and controls the hardware and software resources of a computer system used for pathogen risk assessment of porcine xenotransplantation, and the operation of the computer program and other software or programs.

[0109] exist Figure 2 In the computer system shown for evaluating the pathogen risk level of porcine xenotransplantation, the user interface 1003 is mainly used to connect to the terminal and communicate data with the terminal; the network interface 1004 is mainly used to communicate data with the backend server; the processor 1001 can be used to call the computer program stored in the memory 1005.

[0110] In this embodiment, the computer system for evaluating the pathogen risk level of porcine xenotransplantation includes: a memory 1005, a processor 1001, and a computer program stored in the memory and executable on the processor, wherein:

[0111] When the processor 1001 calls the computer program stored in the memory 1005, it performs the following operations:

[0112] Determine the pathogen type of the pathogen data collected from pig xenotransplanted organs;

[0113] If the pathogen type is the first pathogen type, determining the pathogen risk level of the pathogen data to be tested according to the evaluation score of the pathogen data in the first pathogen index;

[0114] If the pathogen type is the second pathogen type, determining the pathogen risk level of the pathogen data to be tested according to the evaluation score of the pathogen data in the second pathogen index;

[0115] The first pathogen index includes H-index value, infection frequency, pathogenicity, mortality rate, number of countries where the pathogen is distributed, whether it is in an emerging state, transmission mode, whether pigs are the main host, infection status and whether it has been reported by the World Health Organization;

[0116] The second pathogenic indicator includes the first pathogenic indicator, organ transplant relevance, whether it is in a zoonotic state, and the number of cases of pathogen transmission from pigs to humans.

[0117] When the processor 1001 calls the computer program stored in the memory 1005, it performs the following operations:

[0118] When the sum of the evaluation scores of the first pathogen index / the second pathogen index of the pathogen data is within a first numerical range, determining the pathogen risk level to be the first risk level;

[0119] When the sum of the evaluation scores of the first pathogen index / the second pathogen index of the pathogen data is within a second numerical range, determining the pathogen risk level to be the second risk level;

[0120] When the sum of the evaluation scores of the first pathogen index / the second pathogen index of the pathogen data is within a third numerical range, determining the pathogen risk level to be the third risk level;

[0121] The first numerical interval is larger than the second numerical interval and larger than the third numerical interval.

[0122] Although the preferred embodiments of the present application have been described, it will be understood by those skilled in the art that the embodiments of the present application may be provided as methods, systems, or computer program products. Therefore, the present application may take the form of a complete hardware embodiment, a complete software embodiment, or an embodiment combining software and hardware. Furthermore, the present application may take the form of a computer program product implemented on one or more computer-usable storage media (including but not limited to magnetic disk storage, CD-ROM, optical storage, etc.) containing computer-usable program code.

[0123] The present application is described with reference to the flowcharts and / or block diagrams of the methods, devices (systems), and computer program products according to the embodiments of the present application. It should be understood that each process and / or box in the flowchart and / or block diagram, as well as the combination of the processes and / or boxes in the flowchart and / or block diagram, can be implemented by computer program instructions. These computer program instructions can be provided to a processor of a general-purpose computer, a special-purpose computer, an embedded processor, or other programmable data processing device to produce a machine, so that the instructions executed by the processor of the computer or other programmable data processing device generate instructions for implementing the steps in the process. Figure 1 a process or multiple processes and / or boxes Figure 1 A device that provides the functions specified in a block or multiple blocks.

[0124] These computer program instructions may also be stored in a computer readable memory that can direct a computer or other programmable data processing device to work in a specific manner, so that the instructions stored in the computer readable memory produce an article of manufacture comprising an instruction device, which implements the process Figure 1 a process or multiple processes and / or boxes Figure 1The function specified in one or more boxes.

[0125] These computer program instructions can also be loaded onto a computer or other programmable data processing device so that a series of operational steps are executed on the computer or other programmable device to produce a computer-implemented process, thereby providing the instructions executed on the computer or other programmable device for implementing the process. Figure 1 a process or multiple processes and / or boxes Figure 1 A step that specifies a function in one or more boxes.

[0126] It should be noted that in the claims, any reference signs placed between brackets shall not be construed as limiting the claims. The word "comprising" does not exclude the presence of components or steps not listed in the claim. The word "a" or "an" preceding a component does not exclude the presence of a plurality of such components. The present application may be implemented by means of hardware comprising several different components and by means of a suitably programmed computer. In a unit claim enumerating several means, several of these means may be embodied by one and the same item of hardware. The use of the words first, second, and third etc. does not indicate any order. These words may be interpreted as names.

[0127] Although the preferred embodiments of the present application have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present application.

[0128] Obviously, those skilled in the art may make various changes and modifications to this application without departing from the spirit and scope of this application. Thus, if these modifications and variations of this application fall within the scope of the claims of this application and their equivalents, this application is intended to include these modifications and variations.

Claims

1. A method for determining the risk level of pig xenotransplant pathogens, characterized in that: The method comprises the following steps: Determine the pathogen type of the pathogen data collected from pig xenotransplanted organs; If the pathogen type is the first pathogen type, determining the pathogen risk level of the pathogen data to be tested according to the evaluation score of the pathogen data in the first pathogen index; If the pathogen type is the second pathogen type, determining the pathogen risk level of the pathogen data to be tested according to the evaluation score of the pathogen data in the second pathogen index; The first pathogen index includes H-index value, infection frequency, pathogenicity, mortality rate, number of countries where the pathogen is distributed, whether it is in an emerging state, transmission mode, whether pigs are the main host, infection status and whether it has been reported by the World Health Organization; The second pathogenic indicator includes the organ transplant relevance of the first pathogenic indicator, whether it is in a zoonotic state, and the number of cases of pathogen transmission from pigs to humans.

2. The method according to claim 1, wherein The steps for determining the pathogen risk level of the pathogen data to be tested of the first pathogen type or the second pathogen type include: When the sum of the evaluation scores of the first pathogen index / the second pathogen index of the pathogen data is within a first numerical range, determining the pathogen risk level to be the first risk level; When the sum of the evaluation scores of the first pathogen index / the second pathogen index of the pathogen data is within a second numerical range, determining the pathogen risk level to be the second risk level; When the sum of the evaluation scores of the first pathogen index / the second pathogen index of the pathogen data is within a third numerical range, determining the pathogen risk level to be the third risk level; The first numerical interval is larger than the second numerical interval and larger than the third numerical interval.

3. The method according to claim 2, wherein The first risk level indicates that the pathogen data to be tested is a high-risk pathogen that requires routine monitoring, the second risk level indicates that the pathogen data to be tested is a medium- or high-risk pathogen that requires dynamic monitoring based on epidemiology, and the third risk level indicates that the pathogen data to be tested is a low-risk pathogen that does not require monitoring.

4. A risk assessment model for pig xenotransplant pathogens, characterized in that: The risk assessment model for porcine xenotransplant pathogens includes: The first pathogen index used to score the pathogen data of the first pathogen type, including H-index value, infection frequency, pathogenicity, mortality rate, number of countries where the pathogen is distributed, whether it is in an emerging state, transmission mode, whether pigs are the main host, infection status, and whether it has been reported by the World Health Organization; The second pathogen index used to score the pathogen data to be tested of the second pathogen type includes the first pathogen index, organ transplant relevance, whether it is in a zoonotic state, and the number of cases of pathogen transmission from pigs to humans.

5. The risk assessment model for pig xenotransplantation pathogens according to claim 4, wherein: The first pathogenicity index and the second pathogenicity index include at least two evaluation levels, and the first pathogenicity index or the second pathogenicity index associated with the pathogenicity data to be detected has different corresponding weight scores when it is in different evaluation levels.

6. A first pathogen type scoring table and a second pathogen type scoring table obtained using the risk level determination method for porcine xenotransplant pathogens according to any one of claims 1 to 3 and / or the risk assessment model for porcine xenotransplant pathogens according to any one of claims 4 to 5.

7. A risk level classification table for non-zoonotic porcine xenotransplant pathogens obtained using the risk level determination method for porcine xenotransplant pathogens according to any one of claims 1 to 3 and / or the risk assessment model for porcine xenotransplant pathogens according to any one of claims 4 to 5.

8. A risk level classification table for zoonotic porcine xenotransplant pathogens obtained using the risk level determination method for porcine xenotransplant pathogens according to any one of claims 1 to 3 and / or the risk assessment model for porcine xenotransplant pathogens according to any one of claims 4 to 5.

9. Use of the method for determining the risk level of a porcine xenotransplant pathogen according to any one of claims 1 to 3 and / or the risk assessment model for a porcine xenotransplant pathogen according to any one of claims 4 to 5 in monitoring the pathogen infection status of a donor pig before xenotransplantation.

10. A computer system for evaluating the pathogen risk level of pig xenotransplantation, characterized in that: The computer system for evaluating the risk level of pig xenotransplant pathogens includes: a memory, a processor, and a computer program stored in the memory and executable on the processor. When the computer program is executed by the processor, the steps of the method for determining the risk level of pig xenotransplant pathogens according to any one of claims 1 to 3 are implemented.

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