Cultivation method of phalaenopsis amabilis

Abstract

The invention relates to the technical field of flower cultivation, and discloses a cultivation method of phalaenopsis amabilis, which comprises the following steps: before low-temperature induction treatment, performing admission verification on a plant population by analyzing gas metabolism response characteristics of the plant population in a diagnostic dark environment; after verification is passed, controlled metabolic consumption is performed on the plant population, osmotic pressure stress pulses are cooperatively applied during the period to synchronize root activity, and then synchronization energy accumulation is performed; according to the method, systematic physiological function regulation and control including admission verification are carried out on plant populations, passive adaptation to biological individual differences in traditional cultivation is converted into active and whole-plant-dimension physiological state standardization, and therefore the certainty of the cultivation process is fundamentally converted into industrial process control from biological randomness, and the cultivation efficiency is improved. And the predictability and the consistency of production are improved.

Description

Technical Field

[0001] The invention relates to a cultivation method for Phalaenopsis, and belongs to the technical field of flower cultivation. Background Art

[0002] In the current commercial production practice of Phalaenopsis, in order to meet the concentrated supply needs of specific holiday markets, a standardized linear production process is generally followed, that is, the plants accumulate the necessary biomass through long-term vegetative growth, and then the whole plant is transferred to a low-temperature environment for unified flower induction treatment, and finally enters the flowering stage; this cultivation method has provided basic technical support for the rapid development of the Phalaenopsis industry in the past few decades, and has become the mainstream operating procedure recognized by the industry.

[0003] However, with the rapid expansion of production scale and the increasing market demand for uniform product quality, an inherent and long-accepted limitation of the above-mentioned classic cultivation method has begun to emerge. The reason is that the inherent individual differences in biological populations themselves mean that even under strictly unified management conditions, the levels of carbohydrate accumulation and key enzyme activities used to support flower bud differentiation in different plants after the same vegetative growth period actually show a wide statistical distribution. When this population, which is still in a discrete intrinsic physiological state, is placed as a whole under a unified low temperature signal, the speed and ability of each plant to respond to this signal will show differences, which directly leads to inconsistent flowering time, uneven number of peduncles, and flower quality. To address this problem, production companies have to invest a lot of manpower costs in continuous manual sorting, batch reorganization, and differentiated management. This not only significantly increases the complexity and management costs of the production process, but also fundamentally restricts the yield rate and ultimate economic benefits.

[0004] To alleviate this problem, there are some tentative improvement ideas in the industry, such as trying to make up for the differences by providing additional light or fertilizer to individuals with slower growth. However, this catch-up management method is not only not practical in terms of operation under the production scale of tens of thousands of plants, but also difficult to guarantee due to individual differences in plant absorption capacity. Analysis shows that the fundamental dilemma of the existing technology is: 1. Before applying the key and unified flowering induction signal to the entire group, there is a lack of a pre-processing link that can effectively eliminate or converge the inherent individual differences of organisms; 2. All subsequent remedial management methods attempt to level up on the basis of a group that has already formed differences, which is logically more complicated and costly than unifying downward, and fails to solve the problem of inconsistent initial states. Therefore, how to establish a low-cost and scalable application that can effectively pre-treat the intrinsic physiological state of the entire Phalaenopsis cultivation group before the key flowering induction process begins, and avoid the uncertainty of the subsequent production process caused by individual differences, has become a technical problem to be solved by the present invention. Summary of the Invention

[0005] The present invention provides a cultivation method for Phalaenopsis, the main purpose of which is to solve the problems of subsequent asynchronous flower induction, low yield and complex management caused by the lack of a pre-standardized treatment link for the physiological state of the biological group in the existing cultivation method.

[0006] To achieve the above-mentioned object, the present invention provides a method for cultivating Phalaenopsis, comprising the following steps: first, performing a group health status verification step after the end of the vegetative growth period of the Phalaenopsis plant and before the low-temperature induction treatment, wherein the verification step obtains the metabolic activity of the plant group in the closed environment caused by the diagnostic dark environment pulse; concentration change characteristics; then determine whether the change characteristics meet the access conditions calibrated by the historical data of the healthy population; if and only if the change characteristics meet the access conditions, perform subsequent metabolic reset and root synchronization processing, the metabolic reset and root synchronization including: Step a, performing a controlled metabolic depletion treatment, the metabolic depletion treatment comprising placing the plant population under light conditions below its light compensation point for 5 to 7 consecutive days and completely ceasing fertilization; Step b, during the duration of the controlled metabolic depletion treatment, synergistically applying at least one brief osmotic stress pulse to the cultivation medium of the plant population to perform root activity synchronization treatment; Step c: within 24 hours after the controlled metabolic consumption treatment, perform synchronized energy accumulation treatment, which includes providing saturated light conditions and high phosphorus and potassium fertilizers and lasts for 2 to 3 days; after the synchronized energy accumulation treatment is completed, perform low temperature induction treatment on the plant population.

[0007] Preferably, the group health status verification step, before judging the change characteristics, also includes: determining a dynamic health benchmark for comparison with the change characteristics based on one or more current environmental parameters and the growth stage parameters of the plant group; wherein the one or more current environmental parameters include temperature, and the growth stage parameters include the number of cultivation days.

[0008] Preferably, The concentration change characteristics are The coefficient of determination calculated by linear regression fitting of the concentration versus time curve Characterization; the entry conditions are ,in, It is a threshold determined by analyzing historical data of a healthy population and characterizes the homogeneity of the population.

[0009] Preferably, when the change feature does not meet the admission conditions, but the corresponding change feature When the average concentration change rate meets the activity conditions calibrated by the historical data of the healthy group, the plant group is first subjected to a period of weak light treatment before performing the metabolic reset and root synchronization treatment. After the weak light treatment is completed, the group health status verification step is re-executed.

[0010] Preferably, the illumination condition below the light compensation point in step a is specifically a photosynthetic active radiation intensity of 5 to 15 .

[0011] Preferably, the high phosphorus and potassium fertilizer provided in step c has a nitrogen, phosphorus and potassium mass ratio of 1:2:1.5 to 1:3:2.

[0012] Preferably, the osmotic stress pulse applied in step b is achieved by applying a solution containing neutral salts through an irrigation system.

[0013] Preferably, the neutral salt is potassium sulfate, the concentration of which in the solution is 50 to 150 mg / L, and the duration of each application is 30 to 60 minutes.

[0014] Preferably, the application of the diagnostic dark environment pulse is to subject the plant population to a complete darkness treatment lasting 1 to 2 hours after the daytime light ends.

[0015] Preferably, the low temperature induction treatment comprises lowering the temperature of the environment in which the plant population is located to 16 to 20 degrees Celsius.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. This cultivation method subjects the plant population to a controlled metabolic consumption treatment before low-temperature induction treatment. Using illumination conditions below the light compensation point and cessation of fertilization, the internal physiological states of different plants, regardless of their initial energy reserves, converge to a unified low-level baseline. Subsequently, in a synchronized energy accumulation treatment, all plants starting from the same baseline produce consistent absorption and accumulation responses to the uniformly provided saturated light and nutrients. This process transforms the passive adaptation and subsequent adjustment of individual biological differences in traditional cultivation methods into a pre-emptive physiological state standardization of the entire biological population before the start of the key process. This ensures that the subsequent unified low-temperature induction treatment is no longer faced with a population with a discrete distribution of physiological states, but rather a collection of standardized components with uniform initial conditions, similar to those in industrial production. The determinism of the production process is therefore no longer subject to the inherent randomness of organisms.

[0017] 2. This method adds a step to verify the health status of the plant population before the metabolic reset treatment begins, that is, applying a diagnostic dark environment pulse in a closed environment and obtaining the closed environment caused by the metabolic activity of the population. This step converts a macroscopic, easily measurable gas parameter in the environment into a direct, non-invasive representation of the microscopic physiological state of the group. Analysis of the morphological characteristics of the concentration change curve reveals the homogeneity level of the physiological state within the group. Based on this, the method selects from multiple preset processing paths. This means that the initiation of the cultivation process is no longer based on a fixed time node or empirical judgment, but is based on a decision made based on the current real and global physiological snapshot of the production object. This avoids the risk of introducing groups with unsuitable conditions into the consumable processing link and can perform targeted pre-conditioning on groups with discrete conditions.

[0018] 3. When verifying the health status of a group, the method can also dynamically determine the preset health benchmark for comparison based on the current environmental parameters and plant growth stage parameters. For example, based on the real-time greenhouse temperature, the corresponding healthy respiratory rate benchmark is calibrated. This mechanism makes the diagnostic scale no longer fixed, but can adapt to environmental changes and the growth process of the plant itself in real time, avoiding false negative or false positive misjudgments caused by environmental fluctuations or natural growth of plant biomass. This allows the aforementioned verification steps to always maintain the reliability and fairness of their judgments in the real agricultural environment with long production cycles and multiple changes. During the controlled metabolic consumption treatment of the above-ground parts of the plants, this method can also work together By applying a brief and non-harmful osmotic stress pulse to the cultivation substrate, the lower metabolic activity of the aboveground part creates specific physiological conditions for the root system to respond to external stimuli. The osmotic pressure pulse can induce synchronized compensatory stress germination of the plant root system. As a result, when the cultivation enters the subsequent synchronized energy accumulation stage, not only is the energy reserve state of the aboveground part of the plant group uniform, but the absorption activity of its underground root system also reaches a peak synchronously. Through the coupling of this above-ground and underground processing steps, the method expands the object of synchronization from a single energy reserve state to energy acquisition capacity, realizes the coordination and consistency of physiological functions in the whole plant dimension, and ensures that when fertilizer and water are supplied uniformly, each plant can absorb and transform them with uniform efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the interactive process of a cultivation method of Phalaenopsis according to the present invention; Figure 2 For the present invention, the health of homogeneous groups and discrete groups Concentration change curve comparison chart; Figure 3 Schematic diagram of the principle of standardized regulation of the physiological state of plant groups according to the present invention.

[0020] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. Obviously, the described implementation methods are only part of the implementation methods of the present invention, not all of the implementation methods. Based on the implementation methods in the present invention, all other implementation methods obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0022] The present invention provides a cultivation method for Phalaenopsis, which includes a physiological function regulation stage for a plant group after the end of the vegetative growth period of the Phalaenopsis plants and before low-temperature induction treatment. The stage operationally sequentially includes a group health status verification step, a metabolic reset and root system synchronization treatment step, and a synchronized energy accumulation treatment step; wherein the group health status verification step is used to determine whether the plant group is suitable for entering a subsequent regulation process; the metabolic reset and root system synchronization treatment step is used to achieve the coordinated unification of the energy reserve of the above-ground part of the plant and the absorption capacity of the underground part; and the synchronized energy accumulation treatment step is used to uniformly elevate the standardized plant group to a physiological state suitable for receiving flowering induction.

[0023] In the large-scale cultivation of Phalaenopsis, in order to ensure the synchronization of the flower induction treatment, the physiological state of the plant group needs to be standardized. However, before the implementation of low-temperature induction, there is a lack of effective means to evaluate and unify the physiological differences within the group. To this end, this cultivation method sets a group health status verification step to determine whether the cultivated group is suitable for subsequent consumable treatment without performing destructive sampling. In a specific application, this verification step is performed as follows: after the daytime light ends, first ensure that the greenhouse environment where the cultivation is located is in a closed state, and then apply a diagnostic dark environment pulse with a duration of 1 to 2 hours, that is, complete darkness treatment, to the plant group; during this period, the carbon dioxide ( ) sensor, which continuously records the respiratory metabolic activities of the plant population in a closed environment at a set sampling frequency. Concentration change data; healthy and uniform plant groups have relatively consistent respiration rates. The curve of concentration changing with time will be close to a straight line with a stable slope in morphology, while the curve of the group with serious internal physiological differentiation will show nonlinear characteristics. The concentration-time curve was fitted with linear regression and its coefficient of determination was calculated. , a quantitative index characterizing the homogeneity of the group can be obtained, thereby realizing a non-invasive assessment of the homogeneity of the physiological state within the group.

[0024] In order to ensure that the accuracy of the above-mentioned verification judgment is not affected by fluctuations in environmental conditions, the group health status verification step further includes a dynamic health benchmark determination mechanism; the basal respiration rate of the plant group is affected by the ambient temperature and its own total biomass (which can be represented by the number of cultivation days); for this reason, in the initial deployment of this method, through calibration experiments, a parameterized model is established to describe the relationship between the respiration rate of a healthy plant group and the temperature and cultivation days, for example, healthy respiration rate = f (temperature, cultivation days); before each execution of a diagnostic dark environment pulse, the control system obtains the current ambient temperature from the temperature sensor, and combines it with the current number of cultivation days to instantly generate a dynamic health benchmark that matches the current state by calling the aforementioned model; accordingly, the entry condition judgment for group uniformity is to compare the determination coefficient calculated in real time. Is it greater than or equal to a preset uniformity threshold? For example, if the system preset threshold is 0.95, when the real-time calculation value is When the group status is determined to be uniform, it is allowed to enter the subsequent processing flow; this mechanism will be a solid.

[0025] If and only if the change characteristics meet the entry conditions, the subsequent metabolic reset and root synchronization treatment will be performed. This treatment is used to converge the plant groups with different initial states to a unified low-energy physiological baseline and synchronize their root vitality; wherein, step a is a controlled metabolic consumption treatment, which is set to control the photosynthetic active radiation intensity of the plant group's environment within 5 to 15 by regulating the greenhouse lighting system for 5 to 7 consecutive days. Within the range of , the light intensity is lower than the light compensation point of Phalaenopsis, so that the net energy income of the plant is negative, and the internal stored carbohydrates are consumed. At the same time, fertilization is completely stopped during this period; the treatment duration is set based on the fact that 5 days can allow plants with medium energy reserves to reach a lower metabolic level, and 7 days can allow plants with sufficient initial energy reserves to consume their accumulation. At the same time, this duration is not long enough to cause physiological damage to healthy plants; during the execution of the above-mentioned controlled metabolic consumption treatment, step b is collaboratively performed, that is, applying at least one short osmotic stress pulse to the cultivation matrix of the plant group through the irrigation system; the operation is specifically to use a solution containing neutral salts for For short-term irrigation, the neutral salt is potassium sulfate, and its concentration in the solution is controlled at 50 to 150 mg / L, with each application lasting 30 to 60 minutes. The concentration range is set based on the fact that below 50 mg / L, the osmotic pressure generated is insufficient to constitute an effective physiological stimulus, while above 150 mg / L, it may cause salt stress damage to the root system. Therefore, this concentration window is the interval for inducing compensatory stress germination in the root system without causing damage. Since the metabolic activity of the aboveground part of the plant is weak at this time and the root system is sensitive to changes in the external environment, this stress pulse can induce the entire group to synchronously germinate new capillary roots, thereby having consistent absorption capacity in the subsequent nutrient absorption stage.

[0026] After the controlled metabolic consumption treatment is completed, in order to convert the plant population in a standardized low-energy consumption state into a high-energy state suitable for flowering, step c, namely synchronized energy accumulation treatment, is then performed; this treatment is started within 24 hours after the end of the metabolic consumption treatment. The specific operation is to restore the greenhouse environment to conditions suitable for growth, provide saturated light, and apply high-phosphorus and potassium fertilizers for 2 to 3 days; wherein, the mass ratio of nitrogen, phosphorus and potassium elements in high-phosphorus and potassium fertilizers is in the range of 1:2:1.5 to 1:3:2. The basis for setting this ratio is that phosphorus is used to promote flower bud differentiation, and potassium is used to promote carbohydrates. The synthesis and transport of nitrogen is controlled, while the proportion of nitrogen is appropriately reduced to inhibit vegetative growth. After 2 to 3 days of accumulation, the energy levels of all plants starting from the same low baseline are synchronously raised to a consistent platform suitable for flowering. At this point, the physiological function regulation stage is completed, and the plant group can then be subjected to low-temperature induction treatment, for example, the temperature of the environment in which it is located is reduced to 16 to 20 degrees Celsius to initiate a synchronized flowering process. In addition, this cultivation method also includes procedures for special circumstances, that is, when the judgment result of the group health status verification step is that the change characteristics do not meet the uniformity entry conditions (for example, ), but the calculated When the average rate of change of concentration meets the activity conditions calibrated by the historical data of the healthy group, the plant group is first subjected to a period of weak light treatment before performing the metabolic reset and root synchronization treatment; this pre-conditioning procedure is used to preferentially consume the reserves of plants with excessively high energy reserves in the group, so that their status is closer to that of plants with lower reserves, thereby reducing the discreteness of the entire group; after the weak light treatment is completed, the group health status verification step is re-executed until the uniformity of the group meets the entry conditions, and then the standard processing flow is entered. This adaptive conditioning path improves the applicability of this method to groups with different initial states.

[0027] Example 1: The cultivation method of the present invention was applied in a large-scale commercial Phalaenopsis orchid production greenhouse for supplying the Spring Festival market. A batch of 50,000 Phalaenopsis orchid plants that had completed vegetative growth were cultivated in the greenhouse. The plants in this batch appeared to be in good growth condition and of similar specifications. However, the energy reserve levels within the plants for supporting flowering were discretely distributed due to inherent individual differences. If a unified cooling method were directly adopted, the flowering time would be inconsistent, which would fail to meet the concentrated market demand for a specific festival.

[0028] Before the batch of plants enters the scheduled low-temperature induction treatment date, the group health status verification step is started; after the daytime light ends, the greenhouse is sealed and a diagnostic dark environment pulse lasting 2 hours is applied to the plant group; during this period, the carbon dioxide sensor in the greenhouse records the environmental Concentration data show that The concentration-time curve is nonlinear, and the determination coefficient is calculated after linear regression fitting. The value is 0.88, which is lower than the uniformity threshold preset by the system. =0.95, but its average rate of change meets the activity condition, indicating that the group is healthy as a whole but has a high degree of dispersion in its internal physiological state. Based on this judgment, the system did not directly enter the metabolic consumption process, but first automatically executed a 2-day low-light treatment preconditioning program to reduce the physiological state differences within the group. After the preconditioning, the group health status verification step was executed again, and the determination coefficient obtained this time was It is 0.97, which meets the entry conditions and the cultivation process enters the next stage.

[0029] Afterwards, the system automatically starts to perform metabolic reset and root synchronization processing; the greenhouse shading system is activated, reducing the photosynthetic active radiation intensity in the environment and maintaining it at 10 At the same time, the water and fertilizer system stopped all fertilization, and this controlled metabolic consumption treatment lasted for 6 days; on the 3rd and 5th days during this period, the irrigation system executed a short osmotic stress pulse, applying a potassium sulfate solution with a concentration of 100 mg / L to the cultivation medium, each lasting 45 minutes; among them, the low light treatment of the aboveground part weakened the metabolic activity of the plant, creating specific physiological conditions for the root system to respond to external stimuli. The osmotic pressure pulse applied under this condition can effectively induce the roots to produce synchronized stress germination, thereby realizing the coordinated regulation of the two dimensions of the energy state of the aboveground part and the absorption capacity of the underground part.

[0030] After the 6-day metabolic consumption treatment, it immediately entered the synchronized energy accumulation treatment; the greenhouse's shading system was closed, the supplemental lighting system was started to provide saturated light, and at the same time the water and fertilizer system began to apply high-phosphorus and potassium fertilizers with a nitrogen, phosphorus and potassium mass ratio of 1:2.5:2. This process lasted for 3 days; because all plants started from a standardized low-energy consumption baseline and synchronized root activity state, they showed consistent absorption and conversion efficiency for the uniformly supplied light and nutrients; this method of first unifying the physiological state of the group to the same baseline and then improving it synchronously avoided the complexity and uncertainty faced by the traditional method of trying to catch up with a group that already had differences.

[0031] Finally, after completing the synchronized energy product treatment, the 50,000 plants in this batch were transferred as a whole to a low-temperature environment of 18 degrees Celsius for uniform induction treatment; afterwards, the pedicel extraction and flowering process of the entire group showed a high degree of consistency, the flowering synchronization rate reached more than 95%, and the defective rate was less than 3%. The time to market for the entire batch was controlled within the window period of 10 days before the Spring Festival, without the need for a large amount of manual sorting and batch reorganization, and the predictability of the production process and the efficiency of resource utilization were improved.

[0032] Example 2: In order to objectively verify the actual effect of the cultivation method of the present invention on the flowering consistency and product quality of Phalaenopsis, a set of comparative experiments was set up; the experiments were carried out in two independent greenhouse compartments with the same specifications and environmental control systems, each compartment was equipped with a device that could adjust the photosynthetic active radiation intensity to an accuracy of ±2 The system includes a fill light system and an environmental control unit that can control temperature fluctuations within ±0.5 degrees Celsius.

[0033] The experimental materials were 1,000 Phalaenopsis orchid seedlings of the same strain with the same health status, plant age and size, which were randomly divided into a control group and an experimental group, with 500 plants in each group, and placed in the two greenhouse compartments mentioned above respectively; before the start of the experiment, both groups of plants were cultivated under the same standard nutritional growth conditions; the control group adopted a conventional production method, that is, after completing the nutritional growth period, the ambient temperature was directly lowered to 18 degrees Celsius for low temperature induction; before entering the low temperature induction, the experimental group implemented the physiological function regulation method of the present invention, and its key process parameters were set as follows: a controlled metabolic consumption treatment was performed for 6 days, during which the photosynthetic active radiation intensity was maintained at 10 On the 3rd and 5th days, an osmotic stress pulse of potassium sulfate solution with a concentration of 100 mg / L was applied, followed by a 3-day synchronized energy accumulation treatment. After completion, the ambient temperature was lowered to 18 degrees Celsius, the same as the control group.

[0034] After switching to low-temperature induction treatment, the flowering process and quality indicators of the two groups of plants were continuously statistically analyzed, and the results showed significant differences. In terms of the synchronization rate of the beginning of flowering, the experimental group reached 95.6%, while the control group was only 68.2%. This improvement in consistency was directly reflected in the peak flowering period. The experimental group entered the peak flowering period on the 65th day after induction, compared with the 78th day of the control group, 13 days earlier. In terms of the final product quality, the first-class rate of the experimental group reached 88.5%, much higher than the 61.4% of the control group. Correspondingly, the elimination rate of the experimental group was reduced from 15.8% of the control group. to 3.2%; these data differences appear because the experimental group, before the low-temperature induction treatment, effectively unified the physiological baseline of the group through metabolic reset and root synchronization treatment, so that the entire group can produce a more consistent and efficient response to the subsequent unified induction signal; the test results show that, compared with the existing conventional cultivation methods, the application of the method of the present invention can transform the flowering process of the cultivation group into a production process with improved predictability and consistency, which has a positive effect on improving the yield of commercial production and meeting the supply demand in a specific market window period.

[0035] Example 3: This example combines Figures 1 to 3 , a method for cultivating Phalaenopsis is described. Figure 1 As shown, the process begins when the administrator starts the cultivation process. The control system then activates the sensor for environmental monitoring and instructs the greenhouse environment to be sealed. It then applies a dark environment pulse lasting 1 to 2 hours. During this period, the respiratory metabolic activities of the plant population cause the greenhouse environment to The concentration change is fed back to the control system by the sensor in real time, and the control system calculates the Determination coefficient of concentration change curve , and compare it with the preset threshold 0.95 to perform conditional judgment. When the process enters the main path of the alternative treatment sequence shown by alt, a metabolic depletion treatment lasting 5 to 7 days is sequentially performed, during which an osmotic stress pulse is co-administered, followed by an energy accumulation treatment lasting 2 to 3 days, and finally the ambient temperature is lowered to 16 to 20 To enter the flowering induction stage, and when When , it enters the backup path of the alternative processing sequence, that is, it performs low-light pre-processing first, and re-executes the health check step after the processing is completed, and then enters the main processing path after the check passes.

[0036] like Figure 2 As shown, the healthy uniform group (solid line) and the discrete group (dashed line) in the closed environment corresponding to the diagnostic dark environment pulse Typical curve comparison of concentration changes with time (minutes). The horizontal axis represents the time process from the start of dark environment treatment to 120 minutes, and the vertical axis represents the environment Concentration (ppm), where the healthy homogeneous population marked by dots The concentration change curve shows a high degree of linearity, and the determination coefficient of its linear regression fitting is is 0.98, while the discrete groups marked by triangles have The concentration change curve shows obvious nonlinear fluctuations, and its determination coefficient It is only 0.88. This figure intuitively shows how to quantitatively evaluate and distinguish plant groups with different levels of physiological state uniformity by analyzing the morphological characteristics of the macro-environmental gas parameter curves.

[0037] like Figure 3 As shown, in the initial state, the group is composed of plants with different energy reserves (high energy reserve plants) represented by different filling patterns. Their physiological states are discrete, and the corresponding The value is less than 0.95. After the metabolic reset treatment, the physiological state of all plants (low baseline after metabolic consumption) is unified to a uniform low baseline level represented by a dotted box. At this time, the group state converges. After the subsequent energy accumulation treatment, all plants starting from the same baseline consistently reach a uniform high-level energy state represented by a densely filled pattern (high level after energy accumulation). The value can reach a level greater than or equal to 0.97. Finally, when the flowering-inducing signal is applied to this highly uniform group, its flowering process is highly synchronized (synchronous flowering mark), achieving a synchronization rate of more than 95% and a first-class product rate of more than 88%. The figure reveals the core technical logic of the present invention, which is to first converge to a unified baseline and then synchronously improve, to transform a biological random group into an industrial production object with standardized physiological state.

[0038] Example 4: In a specific application, the present cultivation method needs to be applied to a newly introduced Phalaenopsis orchid variety whose physiological stress response characteristics are unknown; in order to determine the applicable concentration of neutral salt when the variety is subjected to an osmotic stress pulse, the following calibration procedure is set up; 300 Phalaenopsis orchid plants of the new variety with uniform growth status and specifications are selected as calibration objects, and they are randomly divided into 6 test sample groups, each with 50 plants; all sample groups are placed in a small growth chamber in which multiple environmental parameters can be independently controlled, and the environmental control system of the growth chamber can independently maintain the light and temperature conditions required for subsequent processes; before the calibration process begins, all sample groups are subjected to conventional nutritional growth under consistent conditions; after the calibration process is started, all 6 sample groups are uniformly subjected to a 7-day controlled metabolic consumption treatment, during which the photosynthetic active radiation intensity is constant at 12 , and stop fertilizing completely.

[0039] On the fourth day of the treatment, each of the six sample groups was subjected to a 45-minute osmotic stress pulse of potassium sulfate solution of different concentrations. Sample group 1 served as the blank control group, and the applied solution concentration was 0 mg / L; sample groups 2 to 6 served as gradient test groups, and the applied solution concentrations were 40 mg / L, 80 mg / L, 120 mg / L, 160 mg / L and 200 mg / L, respectively. 72 hours after the stress pulse application, 10 plants were randomly selected from each sample group. Through image acquisition and analysis, two quantitative evaluation indicators were statistically analyzed, namely the average number of new healthy root tips per plant, which was used to characterize the effectiveness of stress germination, and the damage rate of necrosis or browning symptoms at the root end, which was used to characterize the negative effects of stress.

[0040] The statistical results showed that the average number of new healthy root tips in the blank control group and sample group 2 with a concentration of 40 mg / L did not show a statistical increase compared with the control group; the average number of new healthy root tips in sample groups 3 and 4 with concentrations of 80 mg / L and 120 mg / L was 3.5 times and 4.2 times that of the control group, respectively, and the root damage rate was less than 1%; the number of new healthy root tips in sample group 5 with a concentration of 160 mg / L did not increase further compared with sample group 4, but the root damage rate increased to 5%; the root damage rate in sample group 6 with a concentration of 200 mg / L was 1. The damage rate rose to more than 15%; based on the data from the above calibration test, for this new variety of Phalaenopsis, the applicable concentration range of potassium sulfate solution in its osmotic stress pulse was determined to be 80 mg / L to 120 mg / L; in subsequent large-scale production, the standard operating procedure for this variety set this parameter to 100 mg / L, which can achieve a root germination stimulation effect close to 120 mg / L, and its damage risk is much lower than the concentration level of 160 mg / L, thereby determining the operating parameters of this technical solution for this specific application object.

[0041] Example 5: In a specific deployment scenario, when this cultivation method is first applied to a new production greenhouse or a Phalaenopsis orchid variety for which no data has been accumulated, a parameterized benchmark model calibration procedure needs to be performed before large-scale application; this procedure is used for subsequent group health status verification steps to establish a dynamic health benchmark that matches the specific variety and environmental conditions.

[0042] The calibration procedure is implemented as follows: first, a batch of representative plants of the target variety with healthy physiological status and consistent growth stage are selected and placed in a test compartment with precisely controlled environmental parameters; over a period of time covering the growth stage of the plants from the late vegetative growth to the pre-flowering stage, the ambient temperature in the test compartment is systematically adjusted in a gradient, so that it changes in the range of 15 degrees Celsius to 30 degrees Celsius in steps of 1 degree Celsius, and at each stable temperature point, a standardized diagnostic dark environment pulse is performed on a daily basis. At the same time, a carbon dioxide sensor is used to record the closed environment caused by the respiration of the plant group. In this way, the system collects and stores a series of three-dimensional data points including ambient temperature, cultivation days and the corresponding healthy respiratory rate of the group.

[0043] After completing the above data collection, the mathematical statistics method of multiple regression analysis was used to perform surface fitting on the obtained three-dimensional data points, thereby establishing a function relationship between healthy respiration rate, ambient temperature, and cultivation days: healthy respiration rate = A parameterized benchmark model (temperature, number of cultivation days) is created, or a high-resolution lookup table is generated. This model or lookup table is then embedded in the central control system of the production greenhouse and used as the basis for generating dynamic health benchmarks when verifying the group health status of all subsequent batches. This ensures that the benchmarks used in the verification process reflect the physiological characteristics of the specific production environment and cultivation objects.

[0044] Example 6: In an application for establishing a standardized production baseline for a new variety of Phalaenopsis, in order to accurately adapt the cultivation method to the specific physiological characteristics of the variety, a preliminary control parameter matrix calibration procedure is required; the first part of the procedure is to determine the optimal duration of the controlled metabolic consumption treatment, which is performed as follows: a batch of healthy plants of the new variety are selected, and the photosynthetic active radiation intensity is uniformly 12 After the controlled metabolic consumption treatment, the soluble sugar content of randomly selected leaf samples was analyzed every 24 hours to monitor the consumption process of the plant's internal energy reserves; the experimental data showed that the soluble sugar content in the leaves decreased linearly within the first 4 days, the rate of decline slowed down from the 5th to the 6th day, and entered a plateau period with a content change rate close to zero after the 6th day; accordingly, in order to achieve sufficient energy consumption in this variety without causing excessive stress, the baseline duration of its controlled metabolic consumption treatment was determined to be 6 days.

[0045] The second part of the protocol aims to calibrate the threshold value of the coefficient of determination for judging the homogeneity of the population. The implementation method is as follows: first, three test groups with known discreteness levels are artificially constructed. Group A consists of 50 plants with completely identical growth stages, group B consists of 35 identical plants mixed with 15 plants with slight growth differences, and group C consists of 25 identical plants mixed with 25 plants with obvious growth differences; the three test groups are independently subjected to diagnostic dark environment pulses, and their Determination coefficient of concentration change curve The measurement results show that the The value is stable above 0.98, and the The values ​​ranged from 0.94 to 0.96, while those of group C The value is lower than 0.90; considering that in production, the dispersion of group B is still within the allowable range, while the dispersion of group C needs intervention, the admission judgment threshold used to distinguish whether the group needs to enter the low-light pretreatment The two independent calibration processes above established a control parameter matrix for this new variety, including clear process parameters and quantitative judgment criteria. This matrix was subsequently used to guide all subsequent commercial production batches of this variety, transforming a general technical method into a standardized, precisely repeatable operating process for specific cultivation targets.

[0046] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention.

[0047] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not limiting. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for cultivating Phalaenopsis, characterized in that: The method includes: first, performing a group health status verification step after the end of the vegetative growth period of the Phalaenopsis plant and before the low temperature induction treatment, wherein the verification step obtains the closed environment health status caused by the metabolic activity of the plant group during the diagnostic dark environment pulse. concentration change characteristics; then determine whether the change characteristics meet the access conditions calibrated by the historical data of the healthy population; if and only if the change characteristics meet the access conditions, perform subsequent metabolic reset and root synchronization processing, the metabolic reset and root synchronization including: Step a, performing a controlled metabolic depletion treatment, the metabolic depletion treatment comprising placing the plant population under light conditions below its light compensation point for 5 to 7 consecutive days and completely ceasing fertilization; Step b, during the duration of the controlled metabolic depletion treatment, synergistically applying at least one brief osmotic stress pulse to the cultivation medium of the plant population to perform root activity synchronization treatment; Step c: within 24 hours after the controlled metabolic consumption treatment, perform synchronized energy accumulation treatment, which includes providing saturated light conditions and high phosphorus and potassium fertilizers and lasts for 2 to 3 days; after the synchronized energy accumulation treatment is completed, perform low temperature induction treatment on the plant population.

2. The method for cultivating Phalaenopsis according to claim 1, wherein: The group health status verification step, before determining the change characteristics, also includes: determining a dynamic health benchmark for comparison with the change characteristics based on one or more current environmental parameters and the growth stage parameters of the plant group; wherein the one or more current environmental parameters include temperature, and the growth stage parameters include the number of cultivation days.

3. The method for cultivating Phalaenopsis according to claim 1, wherein: The concentration change characteristics are The coefficient of determination calculated by linear regression fitting of the concentration versus time curve Characterization; the entry conditions are ,in, It is a threshold determined by analyzing historical data of a healthy population and characterizes the homogeneity of the population.

4. The method for cultivating Phalaenopsis according to claim 1, wherein: When the change feature does not meet the admission conditions, but the corresponding change feature When the average concentration change rate meets the activity conditions calibrated by the historical data of the healthy group, the plant group is first subjected to a period of weak light treatment before performing the metabolic reset and root synchronization treatment. After the weak light treatment is completed, the group health status verification step is re-executed.

5. The method for cultivating Phalaenopsis according to claim 1, wherein: The illumination condition below the light compensation point in step a is specifically a photosynthetic active radiation intensity of 5 to 15 .

6. The method for cultivating Phalaenopsis according to claim 1, wherein: The high phosphorus and potassium fertilizer provided in step c has a nitrogen, phosphorus and potassium mass ratio of 1:2:1.5 to 1:3:

2.

7. The method for cultivating Phalaenopsis according to claim 1, wherein: The osmotic stress pulse applied in step b is achieved by applying a solution containing neutral salts through an irrigation system.

8. The method for cultivating Phalaenopsis according to claim 7, wherein: The neutral salt is potassium sulfate, the concentration of which in the solution is 50 to 150 mg / L, and the duration of each application is 30 to 60 minutes.

9. The method for cultivating Phalaenopsis according to claim 1, wherein: The application of the diagnostic dark environment pulse is specifically to subject the plant group to complete darkness for 1 to 2 hours after the end of the daylight.

10. The method for cultivating Phalaenopsis according to claim 1, wherein: The cold induction treatment involves lowering the temperature of the environment in which the plant population is located to 16 to 20 degrees Celsius.

Images