Preservation device and preservation method for maintaining high activity of tumor living tissue

Abstract

The invention discloses a preservation device for maintaining high activity of tumor living tissues. The preservation device comprises a transfer box and a transfer capsule, a plurality of storage cavities are arranged in the transfer box in a linear array mode, and the transfer capsules are detachably installed in the storage cavities. The tumor active tissue placed in the test tube can be stored through the arranged transfer capsule, and meanwhile, during storage, the spring between the built-in cylinder and the outer sleeve can be used for damping, so that mechanical damage to the tumor tissue in the transfer process can be prevented; meanwhile, the device is provided with a corresponding condensation circulator, condensation circulation is conducted on the U-shaped cavity through the arranged condensation circulation assembly, freezing constant-temperature treatment is guaranteed through condensation circulation, and the activity of the tumor tissue in the transfer process is guaranteed.

Description

Technical Field

[0001] The present invention relates to the field of medical devices, and in particular to a preservation device and a preservation method for maintaining high activity of tumor living tissue. Background Art

[0002] Active tumor tissue is a complex system composed of tumor cells and tumor stem cells capable of proliferation, metabolism, angiogenesis, invasion, and metastasis. These cells, including tumor stem cells, have a significant impact on tumor initiation, progression, and therapeutic response. Active tumor tissue contains a variety of cells with specialized biological behaviors, such as tumor stem cells. Studying the characteristics and interactions of these cells helps to uncover the molecular mechanisms of tumorigenesis. By studying the mechanisms regulating tumor stem cell self-renewal and differentiation, we can clarify the key steps in tumor initiation and progression.

[0003] In basic research, active tumor tissue obtained through surgery is directly used for monitoring, and the sample composition obtained through surgery is generally small and precious. However, in the process of transporting samples from the operating room to the freezing refrigerator or the inspection site, the current common method is to transport them through test tubes. Although this can achieve the purpose of transporting and inspecting, when using test tubes alone, it is easy to cause sample inactivation during transportation because of the inability to maintain a low temperature and constant temperature. At the same time, collisions during the process can easily cause mechanical damage, which will also greatly cause the activity of the specimen to decrease, thereby making the final test results inaccurate and affecting scientific research or clinical diagnosis. The current method of transporting samples through test tubes still has defects to a certain extent.

[0004] Therefore, a preservation device and preservation method for maintaining high activity of tumor living tissue are needed to solve the above technical problems. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention discloses a preservation device for maintaining high activity of tumor living tissue. The device can preserve active tumor tissue placed in a test tube. At the same time, during preservation, the provided shock-absorbing mechanism can reduce shock to prevent mechanical damage to the tumor tissue during transportation; at the same time, the device is provided with a corresponding condensation circulation component, which performs freezing and constant temperature treatment to ensure the activity of the tumor tissue during transportation; at the same time, the device completely ensures the low temperature inside through the biomass of the two parts inside and outside.

[0006] To achieve the above technical effects, the present invention designs a preservation device for maintaining high activity of tumor living tissue, comprising: a transfer box and a transfer capsule; the internal linear array of the transfer box is provided with a plurality of storage cavities, and the storage cavities are detachably provided with the transfer capsules;

[0007] The transport capsule comprises an outer sleeve, an inner sleeve and an inner cover; the inner sleeve is sleeved and mounted inside the outer sleeve, and the inner cover is detachably mounted on the outside of the outer sleeve; a U-shaped cavity is provided on the side wall of the inner sleeve, and a flip cover is movably provided on the upper end of the inner portion of the U-shaped cavity; a condensing circulator is fixedly provided inside the inner cover; the two ends of the condensing circulator are respectively connected to the two ports of the U-shaped cavity, and sealing films are provided at both ends of the condensing circulator; the condensing cycle is performed by controlling the installation of the sealing cover and the operation of the condensing circulator to ensure a continuous low temperature and constant temperature inside;

[0008] Furthermore, the inner sleeve and the outer sleeve are flexibly connected by a plurality of springs, and a connecting belt is provided at the ends of the inner sleeve and the outer sleeve for flexible connection;

[0009] Furthermore, the outer upper end of the outer sleeve is provided with a thread to facilitate the mating connection with the fixing ring movably provided at the lower end of the inner cover, so that the inner cover can be firmly fixed; the lower end of the outer sleeve is movably provided with supporting feet;

[0010] Furthermore, an inner handle is fixedly provided at the upper end of the inner cover, and a charging port is fixedly provided on the surface of the inner cover on one side of the inner handle, and the charging port is connected to the condensing circulator through a wire;

[0011] Furthermore, a box cover is movably mounted on one side of the upper end of the transfer box, a control panel is fixedly mounted on the middle of the outer side of the box cover, and a power supply is fixedly mounted inside the box cover; an outer handle is also rotatably mounted on the box cover; and a fixing buckle is provided at the front end of the box cover;

[0012] Furthermore, an indicator light is fixedly installed on one side of the storage cavity on the transfer box; and a scanning probe is fixedly installed on the outer wall of the transfer box.

[0013] In another aspect, the present invention provides a method for maintaining high activity of tumor tissue by a preservation device, which specifically comprises the following steps:

[0014] S1: Clinical tumor sampling and placing the sample into the corresponding test tube (with a code in the test tube);

[0015] S2: Scan the coded test tube in the scanning probe. After the code is scanned, the control module confirms the code and records it. The operator records the corresponding information and position through the control panel. After the recording is completed, the indicator light of the corresponding position lights up.

[0016] S3: Place the corresponding test tube into the built-in cylinder, then cover the inner cover and rotate the fixing ring to fix the inner cover; after fixing, place the entire transport capsule into the storage cavity corresponding to the position of the lit indicator light;

[0017] S4: Place the test tubes in sequence according to the steps in S2-S3. After placement, close the box lid and lock it. Storage is complete.

[0018] S5: When the corresponding tissue needs to be extracted, the operator directly extracts the corresponding tumor tissue according to the record on the control panel. At this time, the indicator light at the corresponding position continues to flash, and the tumor tissue can be extracted. After the extraction, press the control panel again to feedback that the extraction is complete.

[0019] The beneficial effects of the present invention are:

[0020] The present invention designs a preservation device for maintaining high activity of tumor living tissue, comprising: a transfer box and a transfer capsule; the internal linear array of the transfer box is provided with a plurality of storage cavities, and the storage cavities are detachably provided with the transfer capsules;

[0021] The active tumor tissue placed in the test tube can be preserved by the provided transport capsule. At the same time, during preservation, the spring between the inner sleeve and the outer sleeve can be used for shock absorption to prevent mechanical damage to the tumor tissue during transportation. At the same time, the device is provided with a corresponding condensation circulator, which performs condensation circulation on the U-shaped cavity through the provided condensation circulation component, and ensures the freezing constant temperature treatment through the condensation circulation to ensure the activity of the tumor tissue during transportation. At the same time, multiple transport capsules are provided on the device to sample tissues of different parts of the same position or different tissues of different parts, which can meet the sampling needs of different tumor tissues. At the same time, in order to preserve the tumor tissue in an orderly manner, the code is scanned by the provided code scanning probe and the indicator light is turned on to ensure the accuracy of tumor storage and retrieval. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments.

[0023] Figure 1 A schematic diagram of the overall structure of a preservation device for maintaining high activity of tumor living tissue;

[0024] Figure 2 This is a schematic diagram of the overall internal structure of a preservation device for maintaining high activity of tumor living tissue;

[0025] Figure 3 An exploded view of a preservation device that maintains high activity of tumor living tissue;

[0026] Figure 4 A schematic diagram of the structure of a transport capsule in a preservation device for maintaining high activity of tumor living tissue;

[0027] Figure 5A schematic diagram of the explosion structure of a transport capsule in a preservation device for maintaining high activity of tumor living tissue;

[0028] Figure 6 A cross-sectional view of a transport capsule in a preservation device for maintaining high activity of tumor living tissue.

[0029] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0030] 1-Transfer box, 11-Scanning probe, 12-Box cover, 13-Outer handle, 14-Fixed buckle, 15-Control panel, 16-Power supply, 17-Storage chamber, 18-Indicator light, 2-Transfer capsule, 21-Outer sleeve, 211-Thread, 212-Groove, 213-Spring, 214-Connecting belt, 22-Support foot, 23-Inner cover, 231-Inner handle, 232-Charging port, 2321-Wire, 233-Condensation circulator, 234-Circulation pipe, 235-Fixed ring, 236-Sealing film, 24-Built-in cylinder, 241-U-shaped cavity, 242-Flip cover. DETAILED DESCRIPTION

[0031] Example 1

[0032] A device for preserving tumor tissue that maintains high activity, comprising: a transport box 1 and a transport capsule 2; the transport box 1 is provided with a plurality of storage cavities 17 in a linear array, and the transport capsule 2 is detachably mounted inside the storage cavity 17; the transport capsule 2 can be used to preserve active tumor tissue placed in a test tube, and during preservation, a spring between an inner sleeve 24 and an outer sleeve 21 can provide shock absorption to prevent mechanical damage to the tumor tissue during transport.

[0033] Specifically, the transport capsule 2 includes an outer sleeve 21, an inner sleeve 24, and an inner cover 23; the inner sleeve 24 is sleeved and installed inside the outer sleeve 21, and the inner cover 23 is detachably installed outside the outer sleeve 21; the side wall of the inner sleeve 24 is provided with a U-shaped cavity 241, and the upper end of the inner part of the U-shaped cavity 241 is movably provided with a flip cover 242, the flip cover 242 is a circular structure, and a torsion spring is fixedly installed on the rotating shaft of the flip cover 242, and a sealing rubber or sealing ring is fixedly provided on the edge of the flip cover 242 for sealing the inner cover 23 when it is not installed. Sealing function; a condensing circulator 233 is fixedly provided inside the inner cover 23; both ends of the condensing circulator 233 are respectively connected to the two ports of the U-shaped cavity 241, and both ends of the condensing circulator 233 are provided with sealing films 236; by controlling the installation of the sealing cover and the operation of the condensing circulator 233 at the same time, the condensation cycle is performed to ensure the continuous low temperature and constant temperature inside; the condensing circulator 233 belongs to the prior art and will not be explained in detail here. It can be composed of a condensing part (condenser) and a circulating part (circulating pump), which is well known to those skilled in the art.

[0034] In addition, in this embodiment, the inner sleeve 24 and the outer sleeve 21 are flexibly connected by a plurality of springs. The springs can serve as buffers, and corresponding dampers or energy absorption units can also be provided inside the springs to absorb impacts. At the same time, connecting belts 214 are provided at the ports of the inner sleeve 24 and the outer sleeve 21 for flexible connection.

[0035] In this embodiment, in order to better install the inner cover 23 on the outer sleeve 21 and ensure the stability of the connection, a thread 211 is provided on the outer upper end of the outer sleeve 21 to facilitate the cooperation and connection of the fixing ring 235 movably provided at the lower end of the inner cover 23, so that the inner cover 23 can be firmly fixed. When in use, the fixing ring 235 is directly rotated, and the connection and fixation through the thread 211 can be quickly and tightly fitted. Compared with fixing by snapping, the thread 211 can make the inner cover 23 fit tightly downward on the outer sleeve 21, ensuring better sealing. At the same time, support feet 22 are movably installed around the lower end of the outer sleeve 21. When in use, the support feet 22 can be rotated out and used as support.

[0036] In addition, in this embodiment, an inner handle 231 is fixedly provided at the upper end of the inner cover 23, and the inner handle 231 is mainly used to lift the inner cover 23. On one side of the inner handle 231, a charging port 232 is fixedly provided on the surface of the inner cover 23, and the charging port 232 is connected to the condensing circulator 233 through a wire 2321.

[0037] Example 2

[0038] Based on the transfer box 1 described in Example 1 above, a cover 12 is movably mounted on one side of the upper end of the transfer box 1. A control panel 15 is fixedly mounted in the middle of the outer side of the cover 12. A power supply 16 is fixedly mounted inside the cover 12. An outer handle 13 is also rotatably mounted on the cover 12. A fixing buckle 14 is provided at the front end of the cover 12. An indicator light is fixedly mounted on one side of the storage cavity 17 of the transfer box 1. A scanning probe 11 is fixedly mounted on the outer wall of the transfer box 1. The control panel 15 is primarily used to control the operation of the indicator light and the scanning probe 11, and is also used to receive and process scanning information.

[0039] Example 3

[0040] In this embodiment, the present invention provides a method for preserving a preservation device for maintaining high activity of tumor living tissue, which specifically includes the following steps:

[0041] S1: Clinical tumor sampling and placing the sample into the corresponding test tube (with a code in the test tube);

[0042] S2: The coded test tube is scanned in the scanning probe 11. After the code is scanned, the control panel 15 confirms the scanned code and records it. The operator then records the corresponding information and confirms the placement position (the position is the position where the test tube should be placed in the storage chamber 17) through the control panel 15. After the recording is completed, the control panel 15 controls the indicator light of the corresponding position to light up (the light is red).

[0043] S3: Place the corresponding test tube into the inner cylinder 24, then cover the inner cover 23 and rotate the fixing ring 235 to fix the inner cover 23; after fixing, place the entire transport capsule 2 into the storage cavity 17 corresponding to the position of the lit indicator light, and confirm the placement again through the control panel 15, at which time the control panel 15 controls the indicator light to light green;

[0044] S4: Place the test tubes in sequence according to the steps in S2-S3. After placement, close the box cover 12 and lock it, and storage is completed;

[0045] S5: When the corresponding tissue needs to be extracted, the operator directly extracts the corresponding tumor tissue according to the record of the control panel 15 (confirmed by the record of the control panel 15). At this time, the indicator light at the corresponding position continues to flash, and the tumor tissue can be extracted. After the extraction, press the control panel 15 again, and feedback is given that the extraction is completed (the indicator light is off).

[0046] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all details in detail, nor do they limit the invention to only the specific implementation methods described.

Claims

1. A preservation device for maintaining high activity of tumor living tissue, characterized in that: include: A transfer box and a transfer capsule; the internal linear array of the transfer box is provided with a plurality of storage cavities, and the transfer capsule is detachably installed in the storage cavity; The transport capsule includes an outer sleeve, an inner sleeve and an inner cover; the inner sleeve is installed inside the outer sleeve, and the inner cover is detachably installed outside the outer sleeve; the side wall of the inner sleeve is provided with a U-shaped cavity, and the upper end of the inner part of the U-shaped cavity is movably provided with a flip cover; a condensing circulator is fixedly provided inside the inner cover; the two ends of the condensing circulator are respectively connected to the two ports of the U-shaped cavity, and the two ends of the condensing circulator are provided with sealing films; the condensing circulation is carried out by controlling the installation of the sealing cover and the operation of the condensing circulator at the same time to ensure the continuous low temperature and constant temperature inside.

2. A preservation device for maintaining high activity of tumor living tissue according to claim 1, characterized in that: The inner sleeve and the outer sleeve are flexibly connected by a plurality of springs, and connecting belts are provided at the ends of the inner sleeve and the outer sleeve for flexible connection.

3. A preservation device for maintaining high activity of tumor living tissue according to claim 1, characterized in that: The outer upper end of the outer sleeve is provided with a thread to facilitate the matching connection with the fixing ring movably provided at the lower end of the inner cover, so that the inner cover can be firmly fixed; the lower end of the outer sleeve is movably provided with supporting feet.

4. A preservation device for maintaining high activity of tumor living tissue according to claim 1, characterized in that: An inner handle is fixedly provided at the upper end of the inner cover, and a charging port is fixedly provided on one side of the inner handle and located on the surface of the inner cover, and the charging port is connected to the condensing circulator through a wire.

5. A preservation device for maintaining high activity of tumor living tissue according to claim 1, characterized in that: A box cover is movably mounted on one side of the upper end of the transfer box, a control panel is fixedly mounted on the middle of the outer side of the box cover, and a power supply is fixedly mounted inside the box cover; an outer handle is also rotatably mounted on the box cover; and a fixing buckle is provided at the front end of the box cover.

6. A preservation device for maintaining high activity of tumor living tissue according to claim 1, characterized in that: An indicator light is fixedly installed on one side of the storage cavity on the transfer box; and a scanning probe is fixedly installed on the outer side wall of the transfer box.

7. A method for preserving a device for maintaining high activity of tumor living tissue according to any one of claims 1 to 6, comprising the following steps: S1: Clinical tumor sampling and placing the sample into the corresponding test tube (with a code in the test tube); S2: Scan the coded test tube in the scanning probe. After the code is scanned, the control module confirms the code and records it. The operator records the corresponding information and position through the control panel. After the recording is completed, the indicator light of the corresponding position lights up. S3: Place the corresponding test tube into the built-in cylinder, then cover the inner cover and rotate the fixing ring to fix the inner cover; after fixing, place the entire transport capsule into the storage cavity corresponding to the position of the lit indicator light; S4: Place the test tubes in sequence according to the steps in S2-S3. After placement, close the box lid and lock it. Storage is complete. S5: When the corresponding tissue needs to be extracted, the operator directly extracts the corresponding tumor tissue according to the record on the control panel. At this time, the indicator light at the corresponding position continues to flash, and the tumor tissue can be extracted. After the extraction, press the control panel again to feedback that the extraction is complete.

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