Angiography analysis system and analysis method thereof

Abstract

The invention discloses an angiography analysis system and method, and the system comprises a contrast agent input module which is used for preparing an improved contrast agent; the perfusion pump is used for pumping the contrast agent into the vascular system; the scanning module is used for scanning the organism organ filled with the contrast agent to obtain a blood vessel image of the organism organ; the image reconstruction module is used for performing three-dimensional reconstruction on the scanned blood vessel image to generate a three-dimensional model of the blood vessel; the function analysis module is used for analyzing the blood vessel model subjected to three-dimensional reconstruction so as to evaluate the collateral circulation function; and a display module. By improving the formula of the angiographic contrast agent, the microvessel developing capability and the image reconstruction quality are remarkably enhanced, and the method is a basic guarantee for realizing high-resolution perfusion function evaluation. The system can be widely applied to cardiovascular and cerebrovascular disease models, tumor neovascularization imaging and related basic research by cooperating with a standardized perfusion process and a functional analysis module in the system, and has outstanding technical advancement and clinical application potential.

Description

Technical Field

[0001] The present invention relates to the technical field of medical image processing, and in particular to a blood vessel angiography analysis system and an analysis method thereof, which are suitable for evaluating vascular collateral circulation function and assisting clinical diagnosis and treatment. Background Art

[0002] Coronary heart disease (CAD) is a leading cause of death and disability worldwide. Its core pathological mechanism is coronary artery atherosclerosis and the resulting stenosis or occlusion, leading to myocardial ischemia or infarction. Although coronary interventions (such as stent implantation) and surgical bypass surgery have significantly improved prognosis for some patients in recent years, over 20% of patients are unable to receive effective revascularization treatment due to factors such as advanced age, complex comorbidities, and diffuse lesions. Furthermore, even after surgery, some patients still experience in-stent restenosis or graft occlusion, resulting in poor prognosis and high recurrence rates.

[0003] Clinical studies have found that some patients, even with severe coronary artery stenosis or occlusion, do not exhibit obvious symptoms of myocardial ischemia, and their cardiac function remains relatively good. This clinical phenomenon is often attributed to the well-established coronary collateral circulation. Coronary collateral circulation is the process by which the body, after occlusion or stenosis of the original vessel, partially supplies blood to the ischemic area through naturally formed small vessels or pre-existing vascular pathways that remain open at rest. Previous studies have shown that patients with well-established coronary collateral circulation have significantly higher myocardial survival, cardiac function, quality of life, and survival rates than those without.

[0004] However, current clinical assessment methods for coronary collateral circulation remain relatively crude, relying primarily on subjective judgments based on post-coronary angiography imaging. While some scoring systems (such as the Rentrop score) have clinical significance, their results are highly dependent on operator experience, have limited assessment dimensions, and lack quantitative analysis of hemodynamic changes, making them difficult to meet the requirements of modern precision medicine. Angiography is a technique used to observe the vascular system of living organisms and is widely used in medical research and clinical diagnosis. Traditional angiography methods typically require complex procedures and specialized equipment, and have limitations in image acquisition and analysis.

[0005] Therefore, a more efficient and accurate angiography analysis system is needed to meet the needs of modern medical research and clinical applications. Summary of the Invention

[0006] The present application aims at solving the problems of the prior art, and provides an integrated angiography analysis system and an analysis method thereof, which can automatically complete contrast agent injection, scanning imaging, image reconstruction and blood flow function quantitative analysis, and improve the accuracy and objectivity of collateral circulation evaluation.

[0007] To achieve the above object, the present application provides the following technical solutions. In a first aspect, the present application provides an angiography analysis system, comprising: A contrast agent input module for preparing and inputting contrast agent; A perfusion pump connected to the contrast agent input module for continuously and stably pumping contrast agent into the vascular system of a living body; A scanning module for scanning the organs of the living body after perfusion of the contrast agent to obtain vascular images thereof; An image reconstruction module for three-dimensional reconstruction of the vascular images obtained by scanning to generate a three-dimensional model of the blood vessels; A function analysis module for analyzing the three-dimensionally reconstructed blood vessel model to evaluate the function of collateral circulation; A display module for presenting the analysis results, including the vascular reconstruction image, function evaluation parameter chart, etc.

[0008] Further, the contrast agent input module comprises: A contrast agent container for storing contrast agent; A mixing device for mixing different contrast agent components in a certain proportion to obtain the required contrast agent; A sterile syringe for sucking the mixed contrast agent and connecting with the perfusion pump.

[0009] Further, the perfusion pump is a micro perfusion pump, and the perfusion speed and perfusion volume thereof are adjustable, the heart perfusion volume is 25-30 μL, the speed is 10-20 μL / min, and more preferably 10 μL / min.

[0010] Further, in the contrast agent, the volume ratio of MV-122 and MV-diluent is 1:2, and the addition ratio of MV curing agent is 10% of the weight of MV-122 reagent.

[0011] Further, the angiography analysis system further comprises a transparent treatment module for transparent treatment of the organs of the living body after perfusion of the contrast agent, so as to more clearly observe the vascular morphology.

[0012] Further, the function analysis module further comprises a blood vessel classification function, which can classify collateral blood vessels according to the source and shape of the blood vessels.

[0013] Further, the classification of the collateral blood vessels comprises: SpA-1: proximal septal artery (SpA) to distal left coronary artery (LCA), collateral branch running in the anterior papillary muscle; SpA-2: distal SpA - distal to the LCA, close to the ligation point, and running along the endocardial side branch; SpA-3: distal SpA - distal LCA, side branches running at the apex; LCA-1: proximal LCA-distal LCA, collateral branches running in the posterior papillary muscle; LCA-2: distal LCA - distal LCA, collateral vascular plexus across the infarct junction area; RCA-1: distal right coronary artery (RCA) - distal LCA, a side branch running at the apex of the heart.

[0014] Furthermore, the system optimizes side branch formation through median ligation positioning.

[0015] In a second aspect, the present invention provides an analysis method using the above system, comprising the following steps: Control the perfusion pump to inject contrast agent; Start the scanning module to collect images; An image reconstruction module is used to reconstruct a selected area of ​​the scanned blood vessel image; The functional analysis module is used to analyze the reconstructed three-dimensional model and calculate the perfusion volume and diameter parameters of the blood vessels; Output analysis results to the display module.

[0016] Compared with the prior art, the present invention has the following advantages: The present invention integrates key links such as contrast agent input, perfusion control, imaging scanning, image reconstruction, and functional analysis, and proposes a vascular angiography analysis system that reduces human operation errors and improves data processing efficiency. It is particularly suitable for scientific research experiments, animal model analysis, and batch processing of multiple clinical data. Among them, the contrast agent input module uses a new ratio of contrast agent and precise flow rate control, which significantly improves the fluidity and tissue penetration ability of the contrast agent, enabling smooth perfusion of microvessels, and is particularly suitable for observing complex structural areas such as coronary collaterals, cerebellar blood vessels, and tumor neovascularization. After using this contrast agent in a mouse coronary collateral model, Micro-CT imaging showed that the integrity of coronary collateral perfusion was significantly improved, reducing the problems of breakpoints or empty segments caused by incomplete perfusion, making the reconstructed image structure more continuous and the vascular tracking more stable. In addition, this system method can increase the number of collateral branches and make the vascular contours clearer. After reconstruction, it can effectively identify six different types of collaterals, providing a clearer and more reliable imaging basis for the study and classification of coronary collateral mechanisms.

[0017] In summary, the present invention significantly enhances microvascular visualization and image reconstruction quality by improving the formulation of vascular contrast agents. Combined with the standardized perfusion process and functional analysis module in this system, it can be widely used in cardiovascular and cerebrovascular disease models, tumor neovascularization imaging, and related basic research, and has broad clinical application potential. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a system structure diagram of the present invention.

[0019] Figure 2 Flow chart of the analysis method of the present invention.

[0020] Figure 3 Figure 3: Collateral formation and cardiac function of mice with left coronary artery ligation at different positions. A is the Micro-CT imaging result of the mouse heart 7 days after high, middle and low ligation of the left coronary artery. B is the left ventricular ejection fraction (LvEF) and shortening fraction (LvFS) detected by cardiac ultrasound after middle and low ligation.

[0021] Figure 4 The formation and changes of collateral vessels at different times after median ligation in adult mice.

[0022] Figure 5 The classification and diameter of collaterals after median ligation in adult mice. A shows the different types of collaterals marked on the anatomically transparent heart. B is the three-dimensional rendering of the Micro-CT scanned image using Imaris software, showing different types of collaterals (green), donor (red) and recipient (blue) vessels. C is the measurement of the diameters of different collateral vessels based on the Micro-CT scan image.

[0023] Markings in the figure: 1. Contrast agent input module; 1-1. Contrast agent container; 1-2. Mixing device; 1-3. Sterile syringe; 2. Irrigation pump; 3. Scanning module; 4. Image reconstruction module; 5. Functional analysis module; 6. Display module; 7. Transparency processing module. DETAILED DESCRIPTION

[0024] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0025] like Figure 1 As shown, the present invention provides a blood vessel angiography analysis system, comprising: The contrast agent input module 1 is used for preparing and inputting contrast agent; the contrast agent input module 1 comprises: Contrast medium container 1-1: used to store contrast medium and ensure that the contrast medium remains stable and sterile before use; Mixing device 1-2: used to mix the contrast agent of different components in proportion; in this embodiment, MV-122 and MV-diluent are mixed in a volume ratio of 1:2, and MV-curing agent (10% of the weight of MV-122) is added as the contrast agent used in this embodiment; Sterile syringe 1-3: used to suck the mixed contrast agent, and connected with perfusion pump 2 through a connecting tube to ensure the sterile delivery of the contrast agent; Perfusion pump 2: used to continuously and stably pump the contrast agent into the vascular system of the organism, and the perfusion speed and amount can be adjusted, with a cardiac perfusion amount of 25-30 μL and a speed of 10-20 μL / min.

[0026] Scanning module 3: used to scan the organ of the organism after perfusion of the contrast agent to obtain the original blood vessel image; in this embodiment, a Micro-CT scanner is used, and the scanning parameters include a tube current of 145 μA, a voltage of 55 kV, a scanning resolution of 4.0 μm, an exposure time of 250 ms, and a scanning angle of 180 degrees.

[0027] Image reconstruction module 4: used to reconstruct the selected region of the original blood vessel image using the three-dimensional reconstruction software (SCANCO Ray Tracer 4.3.0.9) of the Swiss SCANCO company; Function analysis module 5: used to analyze the reconstructed three-dimensional model to calculate the perfusion volume, diameter and other parameters of the blood vessel; in addition, the collateral vessels can be classified according to their sources and shapes, such as SpA-1, SpA-2, SpA-3, LCA-1, LCA-2 and RCA-1; by analyzing the perfusion volume, diameter and hemodynamic parameters of the blood vessel, the function of the collateral circulation can be evaluated; Display module 6: used to present the analysis results, including the blood vessel reconstruction image and the function evaluation parameter chart, and the user can directly operate each module of the system through the interface to view the real-time data and analysis results; the analysis results can be exported in CSV, PDF and other formats for further analysis and recording by the user.

[0028] In order to further observe the morphology and source of the coronary collateral vessels while preserving the muscle tissue, the system of the present application further comprises a transparent treatment module 7 for transparent treatment of the organ of the organism after perfusion of the contrast agent, so as to more clearly observe the morphology of the blood vessels, and the rehydration treatment can be performed when the internal structure of the ventricle is displayed.

[0029] As Figure 2As shown, the system of the present invention performs dynamic scanning in conjunction with CT equipment after perfusion with injection parameters. The scanning time covers the entire process of the contrast agent entering the receiving area from the feeding artery. The image reconstruction module reconstructs the image into a dynamic sequence. After processing by the functional analysis module, the perfusion time difference and mean grayscale value change of each area are determined to determine whether the collateral circulation is fully established.

[0030] The following is a detailed description of the present invention.

[0031] Taking mice as an example, before using the above system to analyze the organism, the mice are first pre-treated, that is, the blood vessels of the mice are ligated at different positions on the left coronary artery, including the following steps: (1) Mouse anesthesia: Male C57BL / 6J mice, 9 weeks old, weighing 23 ± 1 g, were anesthetized with 0.35 mL of 1.25% avertin intraperitoneally (50% avertin stock solution was prepared by adding 5 g of tribromoethanol to 10 mL of tert-amyl alcohol, shaking thoroughly until the tribromoethanol was completely dissolved, and storing at room temperature in the dark. Before use, dilute to 1.25% avertin working solution with PBS, filter sterilize with a 0.22 μM filter membrane, and store at 4°C in the dark.) (2) Mouse hair removal and skin disinfection: Apply Veet hair removal cream to the neck, chest and left armpit of the mouse. After 1-2 minutes, use a paper towel to fully remove the hair removal cream and disinfect the mouse skin with an iodine cotton swab.

[0032] (3) Fix the mouse's head, limbs, and tail.

[0033] (4) Tracheal intubation: 1) Adjust the ventilator parameters: tidal volume 1.4 mL, respiratory ratio 5:4, and respiratory rate 125 breaths / min.

[0034] 2) Make a 1-cm longitudinal incision in the neck, bluntly separate the subcutaneous fascia and thyroid gland to expose the pretracheal muscles. Separate the muscles along the physiological gaps of the pretracheal muscles to expose the trachea. Insert an endotracheal needle from the side of the mouth to a depth of approximately 2 cm above the sternum.

[0035] 3) Connect the small animal ventilator (Chengdu Taimeng, small animal ventilator, model HX-101E) and secure the endotracheal needle with tape.

[0036] (5) Thoracotomy: A transverse incision of about 1-1.5 cm was made between the 4th and 5th intercostal spaces in the precordial area. The chest muscles were bluntly separated with curved forceps to expose the intercostal spaces. The intercostal spaces were opened with curved forceps. A chest expander (domestic Jiangsu, model XK01) was inserted. The pericardium was torn open to expose the heart.

[0037] (6) Ligation: The left anterior descending branch was ligated with 10-0 suture needle (Nylon (Polyamide 6) single strand, Lingqiao, item number FX001) using micro needle holder (Jiangsu, model J32011). The ligation position was at 0 mm (high), 2.5 mm (middle) or 3.5 mm (low) from the lower edge of the left auricle, with a ligation width of about 1.5 mm and a depth of about 0.5 mm, i.e. the needle was visible through the myocardium. The line was crossed gently, and the area below the ligation line was observed to be pale. Then, a surgical knot was tied.

[0038] (7) Suture: The chest muscles were closed and reset using a curved forceps, and the chest and neck skin were sutured using 6-0 absorbable thread (Shanghai Jinhuang, model R611). The chest was gently squeezed to expel the gas in the chest, thereby avoiding pneumothorax.

[0039] (8) Mouse recovery: After the respirator was stopped, the breathing state of the mouse was observed. If the breathing frequency was more than 100 times per minute and the rhythm was regular, the mouse could be completely separated from the respirator. Then, the mouse was placed on a 35-37℃ heat pad, and the breathing rhythm of the mouse was observed. If the breathing rhythm was regular and the frequency gradually increased, the tracheal cannula needle was pulled out after about 10 minutes. If the breathing was weak, the respirator was continued to support until the mouse could be separated from the respirator.

[0040] Subsequently, using the system described in the present application, the coronary collateral vessels of the heart of an adult mouse were perfused using a contrast agent input module and a perfusion pump, specifically: (1) The mouse heart was perfused at 0, 3, 7, 14, 21, 28, 60 and 120 days after coronary ligation, respectively.

[0041] (2) Anesthesia: The mouse was anesthetized by intraperitoneal injection of 0.4 mL of 1.25% alverine.

[0042] (3) Heparin anticoagulation: 1% heparin (prepared with ddH2O) was injected intravenously through the penis using an insulin syringe (Kangdeli, model 1 mL U-40) to take 100 μL of heparin solution. Heparin anticoagulation was performed for 3 minutes.

[0043] (4) Sacrifice and fixation: The mouse was euthanized by 1.25% alverine anesthesia, and the limbs were fixed with a needle.

[0044] (5) Exposure of the heart: The tissue below the diaphragm was cut open, and the diaphragm on both sides was fixed with a needle. Then, the diaphragm was cut open to expose the chest cavity, the ribs were cut open along the middle axillary line, and the chest cavity was fixed with a needle. The heart was flushed with PBS.

[0045] (7) Exposure of the aorta: A 6-0 non-absorbable surgical suture (Source Likang, multifilament silk thread) about 3 cm long was inserted from the lower thoracic aorta, and a slipknot was tied.

[0046] (8) Aortic perfusion of the dilatation solution: 1) Prepare 10% sodium nitroprusside solution: Dissolve 50 mg of sodium nitroprusside (Sigma-Aldrich, Cat. No. 71778-25G) in PBS and dilute to 500 mL. Submerge the inlet of a peristaltic pump (LongerPump, Cat. No. BT100-1F) in the solution. Connect the outlet to an indwelling cannula (Kandlai, Model 26GY). Remove the cannula tip and adjust the peristaltic pump speed to 6 mL / min. Fill the cannula with the solution, thoroughly removing any air from the cannula.

[0047] 2) Insert the catheter into the thoracic aorta to a depth of approximately 2 cm and secure it. Tie the aorta with the previously inserted 6-0 non-absorbable surgical suture. Continuously perfuse the ductal expansion solution at 6 mL / min for approximately 10 minutes.

[0048] (9) During the perfusion of the vascular expansion solution, separate the adhesions on the surface of the mouse heart under a microscope to fully expose the aortic arch. Prepare multiple 6-0 non-absorbable surgical sutures, about 3 cm long, to ligate the blood vessels.

[0049] (10) Ligation order: brachiocephalic trunk-left common carotid artery-left subclavian artery-right anterior vena cava-left anterior vena cava.

[0050] (11) Stop perfusing the expansion solution and immerse the liquid inlet end of the peristaltic pump into 4% paraformaldehyde (Servicebio, Product No. G1101). Perfuse at a rate of 6 mL / min for about 3 min.

[0051] (12) Prepare 600 μL of MIRCOFIL angiographic contrast agent (Flow Tech Inc, ratio of MV-122: MV-diluent = 1:2), add 20 μL of curing agent, and quickly mix and aspirate the MIRCOFIL contrast agent using a 1 mL syringe (Conlilai) with the needle removed to expel air bubbles.

[0052] (13) Connect the 1 mL syringe containing the inhaled angiographic contrast agent to the indwelling needle and microinjection pump (Rayward, model R462), and pump the MIRCOFIL contrast agent into the aorta at a rate of 200 μL / min. After about 1 minute, when the yellow angiographic contrast agent enters the aorta, slow down the injection rate to 10 μL / min and continue for 2.5 minutes. Under the microscope, it can be observed that the microarteries of the heart are fully filled with angiographic contrast agent.

[0053] (14) Rinse the heart with PBS to prevent the yellow contrast agent from adhering to the heart surface. Let it stand at room temperature (25°C) for 30 minutes. After the contrast agent solidifies, remove the heart and place it in 4% paraformaldehyde at 4°C for fixation overnight. Replace it with PBS after 24 hours.

[0054] Then, the heart perfused with MICROFIL contrast agent was scanned by Micro-CT and reconstructed by three-dimensional blood vessels through scanning module, image reconstruction module and functional analysis module, as shown in Figure 3 、 4 which can directly observe the perfusion volume, number and curvature changes of collateral vessels in the infarct area; specifically: (1) The Micro-CT used in the present application is from Swiss SCANCO company, model μCT45, and the scanning parameters are shown in Table 1 below: Table 1. Micro-CT scanning and reconstruction parameters

[0055]

[0056]

[0057] (2) The scanning parameters were set using the SCAN program software: tube current 145 μA, voltage 55 KV, scanning the whole heart, scanning resolution 4.0 μm, exposure time 250 ms, scanning angle 180 degrees. The phantom (Phantom; provided by the equipment manufacturer) was scanned under the same conditions for calibration. The original image was obtained after scanning.

[0058] (3) Image reconstruction: the original image was reconstructed by selecting the region using the three-dimensional reconstruction software (SCANCO Ray Tracer 4.3.0.9) of Swiss SCANCO company. Before reconstruction, the reconstructed image was previewed, and the threshold was set to 200. After setting the parameters, the selected specified folder was edited to start image reconstruction.

[0059] (4) The perfusion volume below the ligation point and the diameter of different types of collateral vessels were calculated using the Evaluaticon analysis software (Swiss SCANCO company).

[0060] Alternatively, in order to further observe the morphology and source of coronary collateral vessels while preserving muscle tissue, the heart of the mouse perfused with MICROFIL contrast agent was transparentized, and when the internal structure of the ventricle was displayed, it could be rehydrated, as shown in A of Figure 5 , specifically: (1) Heart dehydration: the heart was placed in a 15 mL centrifuge tube and dehydrated with gradient alcohol, 10 mL of each liquid, in the following order: 30% ethanol (30 min) - 50% ethanol (30 min) - 75% ethanol (12 h) - 90% ethanol (12 h) - 100% ethanol (36 h, replaced every 12 h).

[0061] (2) Heart clearing solution: Benzyl benzoate (Sigma-Aldrich, Product No. W213802-1 KG) and benzyl alcohol (Sigma-Aldrich, Product No. 108006-1 L) were prepared at a ratio of 1:1 and mixed thoroughly.

[0062] (3) Add 10 mL of clearing solution to the dehydrated heart and shake on a shaker at room temperature for 7 days at 100 rpm.

[0063] (4) Photography after transparentization: Use a microscope (LEICA, model M205 FCA) to take pictures of the transparent heart at a magnification of 10×.

[0064] (5) Heart rehydration: After clearing, the heart was rehydrated in a gradient of alcohol solutions: 100% ethanol (24 h, with the solution changed three times during this period) - 100% ethanol (24 h) - 95% ethanol (24 h) - 75% ethanol (24 h).

[0065] At the same time, Imaris 10.2.0 software was used to perform three-dimensional rendering of cardiac micro-CT images, mark different side branch types (OXFORD INSTRUMENTS, USA), and calculate the side branch diameters, as shown in Figure 2 . Figure 5 As shown in B and C, specifically: (1) Use Imaris File Converter 10.2.0 software to stack the dicom file sets obtained by scanning with the SCANCO μCT45 instrument, set the Voxel Size to 4 μm, and convert them into Imaris format files.

[0066] (2) Imaris format files were processed using Imaris 10.2.0 software. The Surface module was applied to automatically create objects around the surfaces of all blood vessels, and objects around the surfaces of blood vessels of interest were manually created.

[0067] (3) Use the Filament Object Tracer module to automatically fit and track the generation of a cardiovascular model. Select the detection mode "Automatic path algorithm: with loops, based on local intensity contrast, connecting seed points, connecting cell bodies". Set the maximum diameter of the starting point to 571 μm, ensure that the starting point is limited to the aortic arch, and select "cell body mode" for the starting point fitting. Select multi-scale fitting for the seed point fitting, set the minimum seed point diameter to 12.6 μm and the maximum diameter to 104 μm, and ensure that the seed point is fitted at the end of the blood vessel. Use the built-in machine learning dialog box to discard the incorrectly fitted blood vessels and retain the correct blood vessels. Select the blood vessel of interest and obtain the length, mean diameter, and perfusion volume of the blood vessel of interest through the data automatically generated by the "Length", "Mean Diameter", and "Volume" fields.

[0068] Coronary artery collaterals were classified based on the Micro-CT 3D rendering model and transparent heart and blood vessel images. According to the origin and shape of the collateral vessels, there are 6 categories in total, including 3 categories of ventricular septal origin collaterals, 2 categories of left coronary origin collaterals and 1 category of right coronary origin collaterals, which are: SpA-1: proximal SpA - distal LCA, collateral branch running in the anterior papillary muscle.

[0069] SpA-2: Distal SpA - distal to the LCA, close to the ligation point, running along the endocardial side branch.

[0070] SpA-3: Distal SpA - distal LCA, a side branch running at the apex of the heart.

[0071] LCA-1: proximal LCA-distal LCA, collateral branches running in the posterior papillary muscle.

[0072] LCA-2: distal LCA - distal LCA, collateral vascular plexus across the infarct junction area.

[0073] RCA-1: distal RCA-distal LCA, a side branch running at the apex of the heart.

[0074] Finally, the above results are input into the display module for display, including vascular reconstruction images, functional evaluation parameter charts, etc. The analysis results are exported to CSV, PDF and other formats for users to further analyze and record.

[0075] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A angiography analysis system, characterized in that: include: A contrast agent input module (1), used for preparing and inputting contrast agents; a perfusion pump (2), connected to the contrast agent input module (1), and used for pumping the contrast agent into the vascular system of the organism; A scanning module (3) is used to scan the biological organ after the contrast agent is perfused to obtain a blood vessel image thereof; An image reconstruction module (4) is used to perform three-dimensional reconstruction on the scanned blood vessel image to generate a three-dimensional model of the blood vessel; Functional analysis module (5), used to analyze the three-dimensional reconstructed vascular model to evaluate the collateral circulation function; The display module (6) is used to present the analysis results.

2. The angiography analysis system according to claim 1, characterized in that: The contrast agent input module (1) comprises: a contrast medium container (1-1), for storing a contrast medium; A mixing device (1-2) is used to mix different contrast agent components in proportion to obtain the desired contrast agent; A sterile syringe (1-3) is used to aspirate the mixed contrast medium and is connected to an infusion pump (2).

3. The angiography analysis system according to claim 2, wherein: In the contrast agent, the volume ratio of MV-122 and MV-diluent is 1:2, and the addition ratio of MV curing agent is 10% of the weight of MV-122 reagent.

4. The angiography analysis system according to claim 1, wherein: The perfusion volume of the perfusion pump (2) is 25-30 μL, and the perfusion speed is 10-20 μL / min.

5. The angiography analysis system according to claim 1, characterized in that: The angiography analysis system further comprises a transparency processing module (7) for performing transparency processing on the biological organ after the contrast agent is perfused, so as to observe the vascular morphology more clearly.

6. The angiography analysis system according to claim 1, characterized in that: The functional analysis module (5) also includes a blood vessel classification function, which can classify the collateral blood vessels according to the source and shape of the blood vessels.

7. The angiography analysis system according to claim 6, characterized in that: The classification of collateral vessels includes: SpA-1: proximal SpA to distal LCA, collateral branches running in the anterior papillary muscle; SpA-2: distal SpA - distal to the LCA, close to the ligation point, and running along the endocardial side branch; SpA-3: distal SpA - distal LCA, side branches running at the apex; LCA-1: proximal LCA-distal LCA, collateral branches running in the posterior papillary muscle; LCA-2: distal LCA - distal LCA, collateral vascular plexus across the infarct junction area; RCA-1: distal RCA-distal LCA, a side branch running at the apex of the heart.

8. The angiography analysis system according to claim 1, wherein: The system optimizes side branch formation through median ligature positioning.

9. An analysis method using the system according to any one of claims 1 to 8, characterized in that: The following steps are involved: controlling the perfusion pump (2) to inject contrast agent; Starting the scanning module (3) to collect images; An image reconstruction module (4) is used to reconstruct a selected area of ​​the scanned blood vessel image; The functional analysis module (5) is used to analyze the reconstructed three-dimensional model and calculate the perfusion volume and diameter parameters of the blood vessels; A transparency processing module (7) is used to perform transparency processing on the biological organ after the contrast agent is perfused, and the coronary artery side branches are classified based on the three-dimensional rendering model and the transparent heart and blood vessel image; Output the analysis results to the display module (6).

Images