Essential oil skin care product with mood skin care effect and preparation method

Abstract

The invention belongs to the technical field of skin care products, and particularly relates to an essential oil skin care product with an emotion skin care effect and a preparation method. The essential oil skin care product comprises the following components in percentage by weight: 2-3% of yellow chinaberry tree flower essential oil, 1-2% of rose essential oil, 1-2% of sandalwood essential oil, 0.4-0.6% of lemon essential oil, 0.3-0.5% of an essential oil coordinating agent, 4-6% of jojoba oil, 1-1.5% of ceramide, 0.5-1% of hyaluronic acid, 3-5% of PEG-8 beewax and the balance of deionized water. The essential oil skin care product is prepared by combining the yellow chinaberry tree flower essential oil, the rose essential oil, the sandalwood essential oil and the lemon essential oil, so that not only can the anti-aging and anti-wrinkle effects be achieved, but also the effects of relieving anxiety and improving brain pleasure can be achieved through an olfactory pathway, cortisol secretion is inhibited, inhibition of cortisol on skin collagen and elastic fibers is reduced, and the skin care effect is improved. The skin state is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products, and in particular relates to an essential oil skin care product with emotional skin care efficacy and a preparation method thereof. Background Art

[0002] Modern medical research confirms a significant bidirectional regulatory relationship between skin condition and mood swings. When the body experiences negative emotions such as anxiety and stress, abnormal activation of the hypothalamic-pituitary-adrenal axis (HPA axis) leads to excessive cortisol secretion, which in turn inhibits skin fibroblast activity, accelerates collagen degradation and elastic fiber rupture, and causes skin barrier damage, water loss, and exacerbated photoaging. Traditional skincare products often focus on local repair, but ignore the systemic impact of emotional factors on skin health.

[0003] Currently, natural plant-based active ingredients, due to their inherent biological properties, have demonstrated significant advantages in the field of mood-synergistic skincare. These ingredients not only possess excellent safety, but their metabolites can be completely broken down by the body's natural enzyme system, avoiding the cumulative toxicity of synthetic substances. Furthermore, their molecular structure is highly compatible with skin lipids, allowing them to penetrate the stratum corneum without damage for efficient delivery. More importantly, natural plant-based systems typically contain multiple active ingredients that can simultaneously regulate skin cell function and neurotransmitter secretion through multi-pathway synergy. For example, certain volatile substances can influence the emotional center of the brain through the olfactory pathway.

[0004] However, the practical application of natural ingredients still faces two challenges: first, a single plant extract cannot meet the dual needs of mood regulation and skin repair, and its functional limitations restrict the overall efficacy; second, when multiple active ingredients are compounded, polarity differences, volatility imbalances and chemical incompatibility can easily lead to phase separation, oxidative polymerization and inactivation of effective ingredients. Although existing stabilization technologies such as cyclodextrin encapsulation can ensure overall stability, the ingredients inside the encapsulated capsule will still experience phase separation problems, and the active ingredients cannot work together during sustained release. Summary of the Invention

[0005] In order to solve the above technical defects, the present invention has developed an essential oil skin care product with emotional skin care effect and a preparation method. The prepared essential oil skin care product can not only have antioxidant and antibacterial effects on the skin, improve skin cell activity, and achieve anti-aging and anti-wrinkle effects, but also can further improve skin condition by regulating emotions and enhancing brain pleasure.

[0006] An essential oil skin care product with mood-enhancing effects comprises the following components, measured by weight: 2-3% neem flower essential oil, 1-2% rose essential oil, 1-2% sandalwood essential oil, 0.4-0.6% lemon essential oil, 0.3-0.5% essential oil harmonizer, 4-6% jojoba oil, 1-1.5% ceramide, 0.5-1% hyaluronic acid, 3-5% PEG-8 beeswax, and the balance is deionized water. The preparation method of the essential oil coordinator comprises the following steps: dissolving soybean lecithin and cholesterol in anhydrous ethanol, adding motherwort sideroside-starfish saponin composite powder and uniformly dispersing by ultrasonication, then removing ethanol by rotary evaporation to obtain phospholipid-encapsulated composite powder, mixing squalene and fat-soluble rosmarinic acid under a nitrogen atmosphere, adding tocopherol acetate and stirring to obtain an oil phase solution, adding the oil phase solution to the phospholipid-encapsulated composite powder for high-speed emulsification and homogenization, then adding transglutaminase and refrigerating overnight to inactivate the enzyme to obtain the essential oil coordinator.

[0007] A method for preparing an essential oil skin care product having emotional skin care efficacy comprises the following steps: S1: Preparation of essential oil from neem tree flowers; S2: Preparation of Leonurusin-Asteraenin Composite Powder; S3: Preparation of essential oil harmonizer; S4: Place neem essential oil, rose essential oil, sandalwood essential oil and lemon essential oil in a container, add an essential oil coordinator, and evenly disperse them with an ultrasonic frequency of 25-30 kHz. Then add jojoba oil, ceramide, hyaluronic acid, PEG-8 beeswax, and the remainder of deionized water, transfer them to a homogenizer, and homogenize them at 20-30 MPa for 8-10 minutes to obtain an essential oil skin care product.

[0008] Furthermore, step S1 of preparing the essential oil of neem tree flowers specifically comprises the following steps: S1.1: Freshly picked chinaberry flowers are cleaned and placed in a UV sterilizer for sterilization using ultraviolet light. The flowers are then transferred to a container and pulverized and pulped with deionized water 4-6 times the mass of the chinaberry flowers to obtain chinaberry flower pulp. S1.2: adding 0.1-0.3% of a complex enzyme to the chinaberry flower pulp, stirring evenly, adjusting the pH to 5-5.5, and then performing enzymatic hydrolysis at 40-45°C for 2-3 hours to obtain enzymatically hydrolyzed chinaberry flower pulp; S1.3: Place the enzymatically hydrolyzed chinaberry flower pulp in a distillation container, heat it to 95-97°C, and then distill it at this temperature for 3.5-4.5 hours. The distilled oil and water vapor are condensed to 20-25°C through a condenser, and then placed in an oil-water separator for oil-water separation, and the oil phase components are collected to obtain chinaberry flower essential oil.

[0009] Furthermore, the preparation of the leonurin-starfish saponin composite powder in step S2 specifically includes the following steps: S2.1: Dissolve starfish saponin in 10-12 times its mass in deionized water at 50-55°C, add laccase, and perform enzymatic hydrolysis under a nitrogen atmosphere for 10-12 hours. After enzyme inactivation at 70-75°C, place the solution in a dialysis bag with a molecular weight cutoff of 3-3.5 kDa and dialyze it in deionized water for 24-30 hours, replacing the deionized water every 8 hours to obtain a dialysate. Freeze-dry the solution and then pulverize it to obtain enzymatically hydrolyzed starfish saponin powder. S2.2: Mix motherwort sideroside and enzymatic hydrolyzed starfish saponin powder in a mass ratio of 1:0.5-0.7, add 25-30 times the mass of deionized water and mix evenly, then transfer to a high-pressure reactor, seal the high-pressure reactor, introduce CO2 until the pressure in the reactor reaches 6-6.5 MPa, then heat to 50-55℃ and keep warm for 2-2.5h, release the pressure and perform freeze-drying, and grind to obtain motherwort sideroside-starfish saponin composite powder.

[0010] Furthermore, step S3, the preparation of the essential oil coordinator, specifically comprises the following steps: S3.1: Add 8-10 parts of soy lecithin and 0.4-0.5 parts of cholesterol to 25-30 parts of anhydrous ethanol, and sonicate until the soy lecithin and cholesterol are completely dissolved. Then, add 15-20 parts of leonurin-starfish saponin composite powder and disperse evenly through sonication. Transfer to a rotary evaporator and completely remove the ethanol at 45-50°C and 70-75 rpm to obtain the phospholipid-encapsulated composite powder. S3.2: Mix 1-2 parts of squalene and 0.3-0.5 parts of fat-soluble rosmarinic acid under a nitrogen atmosphere, add 0.06-0.08 parts of tocopherol acetate, heat to 45-50°C and stir for 30-40 minutes to obtain an oil phase solution, add the oil phase solution to the phospholipid-encapsulated composite powder prepared in step S3.1, transfer to a high-speed disperser, and emulsify at 40-45°C and 7500-8000 rpm for 4-5 minutes to obtain a primary emulsion, add 0.1-0.15wt% of glutamine transaminase to the primary emulsion, stir evenly, refrigerate at 4-5°C overnight, and then inactivate the enzyme at 75-80°C. Then transfer to a high-pressure homogenizer for homogenization to obtain an essential oil coordinator.

[0011] Furthermore, the ultraviolet sterilization condition in step S1.1 is ultraviolet sterilization with a wavelength of 200-250nm for 12-15 minutes.

[0012] Furthermore, in the complex enzyme agent of step S1.2, the mass ratio of xylanase:cellulase:pectinase is 1:3-5:4-6.

[0013] Furthermore, in step S2.1, the mass ratio of starfish saponin to laccase is 1:0.02-0.04.

[0014] Furthermore, the homogenization pressure in step S3.2 is 30-40 MPa, and the homogenization time is 6-8 min.

[0015] The beneficial effects are as follows: 1. The present invention prepares an essential oil skin care product by using an essential oil combination of neem tree flower essential oil, rose essential oil, sandalwood essential oil and lemon essential oil, which can not only have antioxidant and antibacterial effects on the skin, improve skin cell activity, and achieve anti-aging and anti-wrinkle effects, but also promote neurotransmitter secretion through the olfactory pathway, enhance brain GABA activity, relieve anxiety, and enhance brain pleasure, thereby inhibiting the secretion of cortisol, greatly reducing the inhibitory effect of cortisol on skin collagen and elastic fibers, and improving the condition of the skin.

[0016] 2. The present invention prepares a leonurin-starfish saponin composite powder by compounding and assembling leonurin and starfish saponin, and then phospholipid-coated the composite powder with soybean lecithin and cholesterol. The prepared phospholipid-coated composite powder is emulsified and blended with antioxidant ingredients squalene, fat-soluble rosmarinic acid and tocopherol acetate, and a cross-linked structure is formed under the action of transglutaminase. The essential oil coordinator is then homogenized into small particles. When the essential oil coordinator is added to a composition of neem flower essential oil, rose essential oil, sandalwood essential oil and lemon essential oil, the phase separation of essential oils of different components during storage can be avoided, and the stability of essential oil skin care products composed of a blend of essential oils of different components can be greatly improved.

[0017] 3. The present invention treats starfish saponin with an enzyme, then mixes it with leonurin and places it in a subcritical environment of 6-6.5 MPa to induce assembly, so that the structures of the two are unfolded and cross-linked, thereby preparing leonurin-starfish saponin composite powder. Compared with the structure of a single component, leonurin and leonurin can better stabilize essential oils of different components, thereby further improving the stability of essential oil skin care products. DETAILED DESCRIPTION

[0018] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0019] Unless otherwise specified, the parts in the following examples are by weight.

[0020] Example 1 The invention discloses an essential oil skin care product with mood-enhancing skin care effects, comprising the following components, by weight percentage: 2% neem flower essential oil, 1% rose essential oil, 1% sandalwood essential oil, 0.4% lemon essential oil, 0.3% essential oil harmonizer, 4% jojoba oil, 1% ceramide, 0.5% hyaluronic acid, 3% PEG-8 beeswax, and the balance being deionized water.

[0021] A method for preparing an essential oil skin care product with emotional skin care efficacy comprises the following steps: S1: Preparation of Neem Flower Essential Oil S1.1: Freshly picked chinaberry flowers were cleaned and placed in a UV sterilizer for 12 minutes using 200 nm ultraviolet light. The flowers were then transferred to a container and pulverized with deionized water (4 times the mass of the chinaberry flowers) to obtain chinaberry flower pulp. S1.2: Add 0.1% of a complex enzyme to the chinaberry flower pulp, stirring evenly. The complex enzyme is prepared by mixing xylanase, cellulase, and pectinase in a ratio of 1:3:4. The pH is adjusted to 5, and then enzymatic hydrolysis is performed at 40°C for 2 hours to obtain enzymatically hydrolyzed chinaberry flower pulp. S1.3: The enzymatically hydrolyzed chinaberry flower pulp is placed in a distillation container, heated to 95°C and then distilled for 3.5 hours. The distilled oil and water vapor is condensed to 20°C through a condenser and then placed in an oil-water separator for oil-water separation. The oil phase components are collected to obtain chinaberry flower essential oil.

[0022] S2: Preparation of Leonurusin-Asteraenin Composite Powder S2.1: Dissolve starfish saponin in 10 times its mass in 50°C deionized water, add laccase at a mass ratio of 1:0.02, and perform enzymatic hydrolysis under nitrogen for 10 h. After enzyme inactivation at 70°C, place the solution in a dialysis bag with a molecular weight cutoff of 3 kDa and dialyze it in deionized water for 24 h. Replace the deionized water every 8 h to obtain the dialysate. Freeze-dry the solution and then grind it into powder to obtain enzymatic hydrolyzed starfish saponin powder. S2.2: Mix motherwort sideroside and enzymatic hydrolyzed starfish saponin powder in a mass ratio of 1:0.5, add 25 times the mass of deionized water and mix evenly, then transfer to a high-pressure reactor, seal the high-pressure reactor, introduce CO2 until the pressure in the reactor reaches 6 MPa, then heat to 50℃ and keep warm for 2h, release the pressure and perform freeze-drying, and grind to obtain motherwort sideroside-starfish saponin composite powder.

[0023] S3: Preparation of essential oil harmonizer S3.1: 8 parts of soy lecithin and 0.4 parts of cholesterol were added to 25 parts of anhydrous ethanol and sonicated until the soy lecithin and cholesterol were completely dissolved. Then, 15 parts of the motherwort-starfish saponin composite powder was added and dispersed evenly through sonication. The mixture was transferred to a rotary evaporator and the ethanol was completely removed at 45°C and 70 rpm to obtain the phospholipid-encapsulated composite powder. S3.2: Mix 1 part of squalene and 0.3 parts of fat-soluble rosmarinic acid under a nitrogen atmosphere, add 0.06 parts of tocopherol acetate, heat to 45°C and stir for 30 minutes to obtain an oil phase solution, add the oil phase solution to the phospholipid-encapsulated composite powder prepared in step S3.1, transfer to a high-speed disperser, and emulsify at 40°C and 7500 rpm for 4 minutes to obtain a primary emulsion, add 0.1wt% of glutamine transaminase to the primary emulsion and stir evenly, refrigerate at 4°C overnight, and then inactivate the enzyme at 75°C. Then transfer to a high-pressure homogenizer for homogenization at a homogenization pressure of 30 MPa and a homogenization time of 6 minutes to obtain an essential oil coordinator.

[0024] S4: According to the raw material ratio, 2 parts of neem flower essential oil, 1 part of rose essential oil, 1 part of sandalwood essential oil and 0.4 parts of lemon essential oil were placed in a container, 0.3 parts of essential oil coordinator were added, and ultrasonic dispersion was carried out at an ultrasonic frequency of 25 kHz. Then, 4 parts of jojoba oil, 1 part of ceramide, 0.5 parts of hyaluronic acid, 3 parts of PEG-8 beeswax, and 79.2 parts of deionized water were added, and the mixture was transferred to a homogenizer and homogenized at 20 MPa for 8 minutes to obtain an essential oil skin care product.

[0025] Example 2 The invention discloses an essential oil skin care product with mood-enhancing skin care effects, comprising the following components by weight: 3% neem flower essential oil, 2% rose essential oil, 2% sandalwood essential oil, 0.6% lemon essential oil, 0.5% essential oil harmonizer, 6% jojoba oil, 1.5% ceramide, 1% hyaluronic acid, 5% PEG-8 beeswax, and the balance being deionized water.

[0026] A method for preparing an essential oil skin care product with emotional skin care efficacy comprises the following steps: S1: Preparation of Neem Flower Essential Oil S1.1: Freshly picked chinaberry flowers were cleaned and placed in a UV sterilizer for 12 minutes using 200 nm ultraviolet light. The flowers were then transferred to a container and pulverized with deionized water (6 times the mass of the chinaberry flowers) to obtain chinaberry flower pulp. S1.2: Add 0.3% of a complex enzyme to the chinaberry flower pulp, stirring evenly. The complex enzyme is prepared by mixing xylanase, cellulase, and pectinase in a ratio of 1:5:6. Adjust the pH to 5, and then perform enzymatic hydrolysis at 40°C for 2 hours to obtain enzymatically hydrolyzed chinaberry flower pulp. S1.3: The enzymatically hydrolyzed chinaberry flower pulp is placed in a distillation container, heated to 95°C and then distilled for 3.5 hours. The distilled oil and water vapor is condensed to 20°C through a condenser and then placed in an oil-water separator for oil-water separation. The oil phase components are collected to obtain chinaberry flower essential oil.

[0027] S2: Preparation of Leonurusin-Asteraenin Composite Powder S2.1: Dissolve starfish saponin in 12 times its mass of deionized water at 50°C, add laccase at a mass ratio of 1:0.04, and perform enzymatic hydrolysis under a nitrogen atmosphere for 10 h. After enzyme inactivation at 70°C, place the solution in a dialysis bag with a molecular weight cutoff of 3 kDa and dialyze it in deionized water for 24 h. Replace the deionized water every 8 h to obtain a dialysate, freeze-dry, and then grind to obtain enzymatically hydrolyzed starfish saponin powder. S2.2: Mix motherwort sideroside and enzymatic hydrolyzed starfish saponin powder in a mass ratio of 1:0.7, add 30 times the mass of deionized water and mix evenly, then transfer to a high-pressure reactor, seal the high-pressure reactor, introduce CO2 until the pressure in the reactor reaches 6 MPa, then heat to 50℃ and keep warm for 2h, release the pressure and perform freeze-drying, and grind to obtain motherwort sideroside-starfish saponin composite powder.

[0028] S3: Preparation of essential oil harmonizer S3.1: 10 parts of soy lecithin and 0.5 parts of cholesterol were added to 30 parts of anhydrous ethanol and sonicated until the soy lecithin and cholesterol were completely dissolved. Then, 20 parts of the motherwort-starfish saponin composite powder were added and dispersed evenly through sonication. The mixture was transferred to a rotary evaporator and the ethanol was completely removed at 45°C and 70 rpm to obtain the phospholipid-encapsulated composite powder. S3.2: Mix 2 parts of squalene and 0.5 parts of fat-soluble rosmarinic acid under a nitrogen atmosphere, add 0.08 parts of tocopherol acetate, heat to 45°C and stir for 30 minutes to obtain an oil phase solution, add the oil phase solution to the phospholipid-encapsulated composite powder prepared in step S3.1, transfer to a high-speed disperser, and emulsify at 40°C and 7500 rpm for 4 minutes to obtain a primary emulsion, add 0.15wt% of glutamine transaminase to the primary emulsion and stir evenly, refrigerate at 4°C overnight, inactivate the enzyme at 75°C, and then transfer to a high-pressure homogenizer for homogenization at a homogenization pressure of 30 MPa and a homogenization time of 6 minutes to obtain an essential oil coordinator.

[0029] S4: According to the raw material ratio, 3 parts of neem flower essential oil, 2 parts of rose essential oil, 2 parts of sandalwood essential oil and 0.6 parts of lemon essential oil were placed in a container, 0.5 parts of essential oil coordinator were added, and ultrasonic dispersion was carried out at an ultrasonic frequency of 25 kHz. Then, 6 parts of jojoba oil, 1.5 parts of ceramide, 1 part of hyaluronic acid, 5 parts of PEG-8 beeswax, and 71 parts of deionized water were added, and the mixture was transferred to a homogenizer and homogenized at 20 MPa for 8 minutes to obtain an essential oil skin care product.

[0030] Example 3 The invention discloses an essential oil skin care product with mood-enhancing skin care effects, comprising the following components, by weight percentage: 2% neem flower essential oil, 1% rose essential oil, 1% sandalwood essential oil, 0.4% lemon essential oil, 0.3% essential oil harmonizer, 4% jojoba oil, 1% ceramide, 0.5% hyaluronic acid, 3% PEG-8 beeswax, and the balance being deionized water.

[0031] A method for preparing an essential oil skin care product with emotional skin care efficacy comprises the following steps: S1: Preparation of Neem Flower Essential Oil S1.1: Freshly picked chinaberry flowers were cleaned and placed in a UV sterilizer for 15 minutes using 250nm ultraviolet light. The flowers were then transferred to a container and pulverized with deionized water (4 times the mass of the chinaberry flowers) to obtain chinaberry flower pulp. S1.2: Add 0.1% of a complex enzyme to the chinaberry flower pulp, stirring evenly. The complex enzyme is prepared by mixing xylanase, cellulase, and pectinase in a ratio of 1:3:4. The pH is adjusted to 5.5, and then enzymatic hydrolysis is performed at 45°C for 3 hours to obtain enzymatically hydrolyzed chinaberry flower pulp. S1.3: The enzymatically hydrolyzed chinaberry flower pulp is placed in a distillation container, heated to 97°C and then distilled at this temperature for 4.5 hours. The distilled oil and water vapor is condensed to 25°C through a condenser and then placed in an oil-water separator for oil-water separation. The oil phase components are collected to obtain chinaberry flower essential oil.

[0032] S2: Preparation of Leonurusin-Asteraenin Composite Powder S2.1: Dissolve starfish saponin in 10 times its mass of deionized water at 55°C, add laccase at a mass ratio of 1:0.02, and perform enzymatic hydrolysis under a nitrogen atmosphere for 12 h. After enzyme inactivation at 75°C, place the solution in a dialysis bag with a molecular weight cutoff of 3.5 kDa and dialyze it in deionized water for 30 h. Replace the deionized water every 8 h to obtain a dialysate, freeze-dry, and then grind to obtain enzymatically hydrolyzed starfish saponin powder. S2.2: Mix motherwort sideroside and enzymatic hydrolyzed starfish saponin powder in a mass ratio of 1:0.5, add 25 times the mass of deionized water and mix evenly, then transfer to a high-pressure reactor, seal the high-pressure reactor, introduce CO2 until the pressure in the reactor reaches 6.5 MPa, then heat to 55℃ and keep warm for 2.5h, release the pressure and freeze-dry, and grind to obtain motherwort sideroside-starfish saponin composite powder.

[0033] S3: Preparation of essential oil harmonizer S3.1: 8 parts of soy lecithin and 0.4 parts of cholesterol were added to 25 parts of anhydrous ethanol and sonicated until the soy lecithin and cholesterol were completely dissolved. Then, 15 parts of the motherwort-starfish saponin composite powder was added and sonicated to disperse uniformly. The mixture was transferred to a rotary evaporator and the ethanol was completely removed at 50°C and 75 rpm to obtain the phospholipid-encapsulated composite powder. S3.2: Mix 1 part of squalene and 0.3 parts of fat-soluble rosmarinic acid under a nitrogen atmosphere, add 0.06 parts of tocopherol acetate, heat to 50°C and stir for 40 minutes to obtain an oil phase solution, add the oil phase solution to the phospholipid-encapsulated composite powder prepared in step S3.1, transfer to a high-speed disperser, and emulsify at 45°C and 8000 rpm for 5 minutes to obtain a primary emulsion, add 0.1wt% of glutamine transaminase to the primary emulsion and stir evenly, refrigerate at 5°C overnight, and then inactivate the enzyme at 80°C. Then transfer to a high-pressure homogenizer for homogenization at a homogenization pressure of 40 MPa and a homogenization time of 8 minutes to obtain an essential oil coordinator.

[0034] S4: According to the raw material ratio, 2 parts of neem flower essential oil, 1 part of rose essential oil, 1 part of sandalwood essential oil and 0.4 parts of lemon essential oil were placed in a container, 0.3 parts of essential oil coordinator were added, and ultrasonic dispersion was carried out at an ultrasonic frequency of 30 kHz. Then, 4 parts of jojoba oil, 1 part of ceramide, 0.5 parts of hyaluronic acid, 3 parts of PEG-8 beeswax, and 79.2 parts of deionized water were added, and the mixture was transferred to a homogenizer and homogenized at 30 MPa for 10 minutes to obtain an essential oil skin care product.

[0035] Comparative Example 1: In the formula, the chinaberry flower essential oil was replaced with an equal mass of a composite essential oil, wherein the composite essential oil consisted of rose essential oil, sandalwood essential oil and lemon essential oil in a mass ratio of 1:1:0.4. The rest of the formula and preparation steps were the same as in Example 1.

[0036] Comparative Example 2: In the formula, rose essential oil, sandalwood essential oil and lemon essential oil were replaced with chinaberry flower essential oil of equal mass, and the rest of the formula and preparation steps were the same as in Example 1.

[0037] Comparative Example 3: No essential oil coordinator was added, and the remaining formula and preparation steps were the same as in Example 1.

[0038] Comparative Example 4: The motherwortside-starfish saponin composite powder in step S3.1 was replaced with 10 parts of motherwortside powder and 5 parts of starfish saponin powder. The rest of the formula and preparation steps were the same as those in Example 1.

[0039] Experiment 1: The essential oil skin care products prepared in Examples 1-3 and Comparative Examples 1-2 were used as test samples, and 6 groups of normal BALB / c mice were depilated on their backs, with 3 mice in each group. The skin elasticity (skin rebound coefficient measured by a skin elasticity meter), skin moisture content (measured by a skin moisture tester), and wrinkle depth (measured by a three-dimensional scanner on the skin surface) of the depilated area were tested. The test results were averaged and recorded as the initial value. Then, each group of mice was smeared on the depilated area with one test sample, applied once in the morning and evening every day, 2 ml each time, for 15 consecutive days as the experimental group. A group of mice was left as a blank group, and then the skin elasticity, skin moisture content, and wrinkle depth were tested again. The test results were averaged and recorded as the final value. The change in skin moisture content, skin rebound coefficient, and wrinkle depth = final value - initial value was calculated, as shown in Table 1.

[0040] Table 1: Record of improvement effects of essential oil skin care products on mouse skin

[0041] It can be seen from the data of Examples 1-3 in Table 1 that the essential oil skin care products prepared in the examples of the present application can well improve skin elasticity and skin moisture content, reduce skin wrinkles, and improve skin condition. However, from the data of Comparative Examples 1-2, it can be seen that after replacing the neem flower essential oil with rose essential oil, sandalwood essential oil, and lemon essential oil with each other, the overall effect is reduced compared to Example 1. This proves that the neem flower essential oil has a synergistic effect with rose essential oil, sandalwood essential oil, and lemon essential oil, and can improve the skin condition.

[0042] Experiment 2: Normal BALB / c mice weighing 20-22 g were randomly divided into 6 groups, with 3 mice in each group. The control group was injected with 1 mg / kg of LPS lipopolysaccharide into the abdominal cavity on the 6th day of normal feeding. The other 5 groups were administrated with the essential oil skin care products prepared in Examples 1-3 and Comparative Examples 1-2, respectively. The specific administration steps were as follows: 1 mL of the essential oil skin care product was applied to a 20 cm 3 Each mouse was placed in a different sealed container for aromatherapy for 1 hour, once in the morning and evening every day. Starting from the 6th day, 1 mg / kg of LPS lipopolysaccharide was injected intraperitoneally 1 hour after aromatherapy. The symptoms of mice in each group were observed 12 hours after LPS lipopolysaccharide injection, and then the immobility time of mice in the 5-min forced swimming test was recorded 24 hours later. The results are shown in Table 2.

[0043] Table 2: Record of the effect of essential oil skin care products on alleviating LPS depression in mice

[0044] It can be seen from the data of Examples 1-3 in Table 2 that the essential oil skin care products prepared in the present application can effectively alleviate the depressive behavior induced by the LPS mouse model, and have good mood regulation ability. However, from the data of Comparative Examples 1-2, it can be seen that replacing the neem flower essential oil with rose essential oil, sandalwood essential oil and lemon essential oil with each other will reduce the effect of the essential oil skin care products in alleviating the depressive behavior of mice. It can be proved that the compounding of neem flower essential oil with rose essential oil, sandalwood essential oil and lemon essential oil can synergistically relieve anxiety, reduce depression, and enhance brain pleasure.

[0045] Experiment 3: Take the essential oil skin care products prepared in Examples 1-3 and Comparative Examples 3-4 as samples, and place them at 45°C and room temperature for 3 months, respectively. Three samples are placed at each temperature to observe whether there are abnormal conditions such as precipitation, discoloration, and taste change. As long as one sample has an abnormality, it is unqualified, otherwise it is qualified. The test results are shown in Table 3.

[0046] Table 3: Essential Oil Skin Care Product Stability Test Record

[0047] It can be seen from the data of Examples 1-3 in Table 3 that the essential oil skin care products prepared in the examples of the present application have excellent stability, while it can be seen from Comparative Examples 3-4 that when the essential oil coordinator is not added, the stability of the skin care product is greatly reduced, and the stability of the skin care product is also reduced when the motherwort sideroside and the starfish saponin are not compounded. It can be proved that the essential oil coordinator can improve the stability of the essential oil skin care products composed of a blend of essential oils with different components, and the stability of the essential oil skin care products can be further enhanced by enzymatically treating the starfish saponin and then mixing it with the motherwort sideroside in a subcritical environment of 6-6.5 MPa to induce assembly.

[0048] Experiment 4: Take the essential oil skin care products prepared in Examples 1-3 and add them into the small chamber of the patch test apparatus in an amount of 0.025 mL to obtain patch test samples. The backs of New Zealand rabbits were partially depilated, and the patch test samples were lightly pressed and applied to the depilated area. After 24 hours, the skin reaction of the depilated area was observed. Three groups of New Zealand rabbits were tested for each patch test sample, with 3 rabbits in each group. The results are shown in Table 4.

[0049] Table 4: Record of skin irritation caused by essential oil skin care products

[0050] It can be seen from the data of Examples 1-3 in Table 4 that after the patch test of the essential oil skin care product of the present application, no adverse reactions occurred on the skin of New Zealand rabbits, indicating that the essential oil skin care product prepared in the present application is non-irritating to the skin and has good biocompatibility.

[0051] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. An essential oil skin care product with emotional skin care effect, characterized in that: The invention comprises the following components by weight percentage: 2-3% neem flower essential oil, 1-2% rose essential oil, 1-2% sandalwood essential oil, 0.4-0.6% lemon essential oil, 0.3-0.5% essential oil coordinator, 4-6% jojoba oil, 1-1.5% ceramide, 0.5-1% hyaluronic acid, 3-5% PEG-8 beeswax, and the balance is deionized water; The preparation method of the essential oil coordinator comprises the following steps: dissolving soybean lecithin and cholesterol in anhydrous ethanol, adding motherwort sideroside-starfish saponin composite powder and uniformly dispersing the mixture through ultrasonication, then removing the ethanol through rotary evaporation to obtain a phospholipid-encapsulated composite powder, mixing squalene and fat-soluble rosmarinic acid under a nitrogen atmosphere, adding tocopherol acetate and stirring to obtain an oil phase solution, adding the oil phase solution to the phospholipid-encapsulated composite powder and performing high-speed emulsification and homogenization, then adding transglutaminase and refrigerating overnight to inactivate the enzyme to obtain the essential oil coordinator.

2. A method for preparing the essential oil skin care product with emotional skin care efficacy according to claim 1, characterized in that: The following steps are involved: S1: Preparation of essential oil from neem tree flowers; S2: Preparation of Leonurusin-Asteraenin Composite Powder; S3: Preparation of essential oil harmonizer; S4: Place neem essential oil, rose essential oil, sandalwood essential oil and lemon essential oil in a container, add an essential oil coordinator, and evenly disperse them with an ultrasonic frequency of 25-30 kHz. Then add jojoba oil, ceramide, hyaluronic acid, PEG-8 beeswax, and the remainder of deionized water, transfer them to a homogenizer, and homogenize them at 20-30 MPa for 8-10 minutes to obtain an essential oil skin care product.

3. The method for preparing the essential oil skin care product with emotional skin care efficacy according to claim 2, wherein: Step S1: Preparation of neem flower essential oil, specifically comprising the following steps: S1.1: Freshly picked chinaberry flowers are cleaned and placed in a UV sterilizer for sterilization using ultraviolet light. The flowers are then transferred to a container and pulverized and pulped with deionized water 4-6 times the mass of the chinaberry flowers to obtain chinaberry flower pulp. S1.2: adding 0.1-0.3% of a complex enzyme to the chinaberry flower pulp, stirring evenly, adjusting the pH to 5-5.5, and then performing enzymatic hydrolysis at 40-45°C for 2-3 hours to obtain enzymatically hydrolyzed chinaberry flower pulp; S1.3: Place the enzymatically hydrolyzed chinaberry flower pulp in a distillation container, heat it to 95-97°C, and then distill it at this temperature for 3.5-4.5 hours. The distilled oil and water vapor are condensed to 20-25°C through a condenser, and then placed in an oil-water separator for oil-water separation, and the oil phase components are collected to obtain chinaberry flower essential oil.

4. The method for preparing the essential oil skin care product with emotional skin care efficacy according to claim 3, characterized in that: The conditions for ultraviolet sterilization in step S1.1 are ultraviolet sterilization with a wavelength of 200-250 nm for 12-15 minutes.

5. The method for preparing the essential oil skin care product with emotional skin care efficacy according to claim 3, characterized in that: In the complex enzyme agent of step S1.2, the mass ratio of xylanase: cellulase: pectinase is 1:3-5:4-6.

6. The method for preparing the essential oil skin care product with emotional skin care efficacy according to claim 2, characterized in that: Step S2 is the preparation of the leonurin-starfish saponin composite powder, which specifically comprises the following steps: S2.1: Dissolve starfish saponin in 10-12 times its mass in deionized water at 50-55°C, add laccase, and perform enzymatic hydrolysis under a nitrogen atmosphere for 10-12 hours. After enzyme inactivation at 70-75°C, place the solution in a dialysis bag with a molecular weight cutoff of 3-3.5 kDa and dialyze it in deionized water for 24-30 hours, replacing the deionized water every 8 hours to obtain a dialysate. Freeze-dry the solution and then pulverize it to obtain enzymatically hydrolyzed starfish saponin powder. S2.2: Mix motherwort sideroside and enzymatic hydrolyzed starfish saponin powder in a mass ratio of 1:0.5-0.7, add 25-30 times the mass of deionized water and mix evenly, then transfer to a high-pressure reactor, seal the high-pressure reactor, introduce CO2 until the pressure in the reactor reaches 6-6.5 MPa, then heat to 50-55℃ and keep warm for 2-2.5h, release the pressure and perform freeze-drying, and grind to obtain motherwort sideroside-starfish saponin composite powder.

7. The method for preparing the essential oil skin care product with emotional skin care efficacy according to claim 6, characterized in that: The mass ratio of starfish saponin to laccase in step S2.1 is 1:0.02-0.

04.

8. The method for preparing the essential oil skin care product with emotional skin care efficacy according to claim 2, characterized in that: Step S3 is the preparation of the essential oil coordinator, which specifically comprises the following steps: S3.1: Add 8-10 parts of soy lecithin and 0.4-0.5 parts of cholesterol to 25-30 parts of anhydrous ethanol, and sonicate until the soy lecithin and cholesterol are completely dissolved. Then, add 15-20 parts of leonurin-starfish saponin composite powder and disperse evenly through sonication. Transfer to a rotary evaporator and completely remove the ethanol at 45-50°C and 70-75 rpm to obtain the phospholipid-encapsulated composite powder. S3.2: Mix 1-2 parts of squalene and 0.3-0.5 parts of fat-soluble rosmarinic acid under a nitrogen atmosphere, add 0.06-0.08 parts of tocopherol acetate, heat to 45-50°C and stir for 30-40 minutes to obtain an oil phase solution, add the oil phase solution to the phospholipid-encapsulated composite powder prepared in step S3.1, transfer to a high-speed disperser, and emulsify at 40-45°C and 7500-8000 rpm for 4-5 minutes to obtain a primary emulsion, add 0.1-0.15wt% of glutamine transaminase to the primary emulsion, stir evenly, refrigerate at 4-5°C overnight, and then inactivate the enzyme at 75-80°C. Then transfer to a high-pressure homogenizer for homogenization to obtain an essential oil coordinator.

9. The method for preparing the essential oil skin care product with emotional skin care efficacy according to claim 8, characterized in that: The homogenization pressure in step S3.2 is 30-40 MPa, and the homogenization time is 6-8 min.

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