Natural extracted anti-wrinkle firming composition and application thereof

Abstract

The invention relates to the field of skin care products, and particularly discloses a naturally extracted anti-wrinkle firming composition, which comprises a hydrolyzed ulva extract, an alpinia zerumbet leaf extract, a pyracantha fortuneana fruit extract and an Illicium verum extract. The composition has good adaptability, and can be combined with other cosmetic matrix components to prepare different anti-wrinkle firming cosmetics. The anti-wrinkle and firming composition also has the advantages of mild action, small irritation and excellent anti-wrinkle and firming effects.

Description

Technical Field

[0001] The present invention relates to the field of skin care products, in particular to a naturally extracted anti-wrinkle and firming composition and application thereof. Background Art

[0002] As we age, skin aging problems gradually become apparent, usually manifested as loss of elasticity, decreased firmness, and increased wrinkles. With economic development, people are paying more and more attention to skin aging, and many products specifically designed for anti-aging have appeared on the market, among which anti-wrinkle and firming products are the most widely used.

[0003] However, existing anti-wrinkle and firming products often rely on chemically synthesized ingredients, such as peptides, hyaluronic acid, and retinol. While these ingredients can effectively improve signs of skin aging to a certain extent, long-term use may cause skin irritation and pose certain safety risks. Furthermore, some chemically synthesized ingredients have poor environmental degradation, potentially causing adverse impacts on the ecological environment.

[0004] To address the shortcomings of chemical-based anti-wrinkle and firming products, a number of natural anti-wrinkle and firming products, such as those made with plant extracts, have been introduced. While these natural anti-wrinkle and firming products are relatively safe, they often lack the stability, absorption, and long-lasting effects of synthetic ingredients, resulting in less than ideal anti-wrinkle results.

[0005] In view of the above-mentioned related technologies, the inventors believe that it is necessary to develop a naturally extracted anti-wrinkle and firming composition with anti-wrinkle and firming effects that are no less effective than those of chemically synthesized ingredients while retaining the high safety of naturally extracted plant extracts. Summary of the Invention

[0006] In order to solve the technical defects of the prior art, the present application provides a naturally extracted anti-wrinkle and firming composition and its application In a first aspect, the present application provides a naturally extracted anti-wrinkle and firming composition, which adopts the following technical solution: A naturally extracted anti-wrinkle and firming composition comprising, by weight: 1-50 parts of hydrolyzed Ulva extract; 0.06-1.8 parts of Alpinia glabra leaf extract; 0.1-5 parts of Pyracantha fortuneana fruit extract; Iceland lichen extract 0.01-0.2 parts.

[0007] By adopting the above technical scheme, the composition utilizes the synergistic effect of polysaccharides and amino acids in the hydrolyzed Ulva lactuca extract, flavones and volatile oils in the Alpinia zerumbet leaf extract, polyphenols and vitamin C in the Pyracantha fortuneana fruit extract, and lichen acids and polysaccharides in the Cetralia islandica extract, and can effectively promote collagen synthesis, significantly improve the skin anti-wrinkle and firming effect, and realize multi-target synergistic anti-aging care under the premise of mildness and non-irritation.

[0008] Preferably, the composition comprises, by weight fraction: 20 parts of the hydrolyzed Ulva lactuca extract; 1 part of the Alpinia zerumbet leaf extract; 2 parts of the Pyracantha fortuneana fruit extract; 0.1 part of the Cetralia islandica extract.

[0009] By adopting the above technical scheme, the composition is precisely compounded, and the maximum anti-wrinkle and firming effect is achieved with a small amount of addition.

[0010] Preferably, the preparation method of the hydrolyzed Ulva lactuca extract is as follows: S1: washing and drying the Ulva lactuca, crushing, and sieving to obtain Ulva lactuca powder; S2: enzymatically hydrolyzing the Ulva lactuca powder in step S1 with deionized water and biological enzymes for 2-3 hours, inactivating the enzymes after the enzymatic hydrolysis is completed, and obtaining an enzymatic hydrolysis solution; The biological enzymes are a combination of cellulase, protease, and glucanase. S3: adding anhydrous ethanol in an amount of 15-20 times the weight of the Ulva lactuca powder to the enzymatic hydrolysis solution in step S2, and then performing ultrasonic extraction, filtering the extraction solution, and obtaining a supernatant; S4: freeze-drying the supernatant in step S3 to obtain the hydrolyzed Ulva lactuca extract.

[0011] By adopting the above technical scheme, the extraction method of the hydrolyzed Ulva lactuca is optimized, the active substances of the hydrolyzed Ulva lactuca extract are maximized, and the efficacy of the composition is improved.

[0012] In a second aspect, the present application provides a use of a natural anti-wrinkle and firming composition in the preparation of a cosmetic product.

[0013] Preferably, the cosmetic product is a serum, an essence oil, a cream, a lotion, or an eye cream.

[0014] By adopting the above technical scheme, the composition can be prepared into various cosmetic dosage forms such as facial cleanser, skin toner, and lotion, each dosage form can meet the needs of different skin care scenarios by virtue of the synergistic anti-wrinkle and firming effect of the extracts, and can optimize the release of active ingredients by means of the characteristics of the dosage form, realize the unity of mild care and high-efficiency anti-wrinkle, and provide consumers with diversified anti-aging skin care options.

[0015] In a third aspect, the present application provides a cosmetic product, which adopts the following technical scheme: A cosmetic comprising the naturally extracted anti-wrinkle and firming composition.

[0016] By adopting the above technical solution, the cosmetics are based on a composition containing extracts such as hydrolyzed Ulva glabra and Alpinia zerumbodensis leaves. By utilizing the synergistic effect of various ingredients, it effectively inhibits collagenase and promotes collagen synthesis on the basis of mild effects, giving the skin anti-wrinkle and firming effects. It can also be adapted to a variety of cosmetic dosage forms to meet different skin care needs.

[0017] Preferably, the cosmetic further comprises one or more of a solvent, an emulsifier, an emollient, a moisturizer, a thickener, an antioxidant, a preservative and a fragrance.

[0018] By adopting this technical solution, cosmetics incorporate a variety of auxiliary ingredients, such as solvents and emulsifiers, into the core anti-wrinkle and firming composition. These ingredients work synergistically with the active ingredients to ensure product stability, improve texture and feel, enhance moisturizing and antioxidant properties, and effectively inhibit microbial growth. This results in a product that combines mildness with long-lasting anti-wrinkle and firming benefits, providing users with a superior skincare experience.

[0019] Preferably, the solvent may be one or more of water, butylene glycol and propylene glycol; The emulsifier may be one or more of glyceryl stearate, polysorbate 80 and lecithin; The emollient is one or more of squalane, jojoba oil and shea butter; The moisturizing agent is one or more of glycerin, hyaluronic acid and panthenol; The thickener is one or more of carbomer, xanthan gum and hydroxyethyl cellulose; The antioxidant is one or more of vitamin E, tea polyphenols and coenzyme Q10; The preservative is one or more of phenoxyethanol, chlorphenesin and potassium sorbate; The aromatic agent is one or more of rose essential oil, lavender essential oil and sweet orange essential oil.

[0020] By adopting the above technical solution, preferably solvents, emulsifiers and other ingredients, it is possible to more effectively provide an action environment for the anti-wrinkle and firming composition to adapt to different groups of people, thereby ensuring that the active ingredients achieve anti-wrinkle and firming effects.

[0021] In summary, this application has the following beneficial effects: 1. The composition of the present application utilizes the synergistic effects of polysaccharides and amino acids of hydrolyzed Ulva extract, flavonoids and volatile oils of Alpinia galanga leaves, polyphenols and vitamin C of Pyracantha fruit, and lichen acid and polysaccharides of Icelandic lichen to effectively promote collagen synthesis, significantly improve the anti-wrinkle and firming effects of the skin, and achieve multi-target synergistic anti-aging care.

[0022] 2. The raw materials of the anti-wrinkle and firming composition of the present application are all plant extracts, which are mild and non-irritating and have wider applicability. DETAILED DESCRIPTION

[0023] The present application is further described in detail below with reference to the embodiments.

[0024] The raw materials, consumables and instruments involved in this embodiment are all conventional commercially available products and can be purchased on the market.

[0025] Example 1 A naturally extracted anti-wrinkle and firming composition comprises, by weight, 1 part of hydrolyzed ulva extract, 0.06 parts of Alpinia galanga leaf extract, 0.1 parts of Pyracantha fortuneana fruit extract, 0.01 parts of Iceland lichen extract, and deionized water to make up to 100 parts.

[0026] Among them, the Alpinia glabra leaf extract was purchased from Guangzhou Youran Biotechnology Co., Ltd.; the Pyracantha fortuneana fruit extract was purchased from Foshan Xin'an Chemical Trading Co., Ltd.; and the Iceland lichen extract was purchased from Guangzhou Xincheng Meike Biological Co., Ltd.

[0027] The preparation method of the hydrolyzed Ulva extract in this embodiment is as follows: S1: Wash the Ulva, vacuum dry at 40°C until the moisture content is ≤8%, grind and pass through a 50-mesh sieve to obtain Ulva powder; S2: The Ulva powder in step S1 was added to deionized water at a material-liquid ratio of 1 g:15 mL, and then 0.2% of the powder weight and 0.5% of the cellulase, protease and glucanase at a mass ratio of 2:1:1 were added. The mixture was enzymolyzed at a constant temperature of pH 6.5 and 40°C for 2 h. After the enzymolysis was completed, the enzymes were inactivated to obtain an enzymolysis solution. S3: adding 15 times the weight of the Ulva powder to the enzymatic hydrolyzate of step S2 in anhydrous ethanol, stirring evenly, and then performing ultrasonic extraction at an ultrasonic power of 300 W, an ultrasonic time of 30 min, and an ultrasonic temperature of 45° C. The extract is first coarsely filtered through a 200-mesh filter cloth, and then finely filtered through a 0.4 μm microporous filter membrane to remove impurities to obtain a supernatant; S4: adding 0.4% by mass of mannitol as a freeze-drying protective agent to the supernatant of step S3 and then performing freeze-drying operation. The freeze-dried powder is the hydrolyzed Ulva extract.

[0028] Example 2 A naturally extracted anti-wrinkle and firming composition comprises, by weight, 20 parts of hydrolyzed ulva extract, 1 part of Alpinia galanga leaf extract, 2 parts of Pyracantha fortuneana fruit extract, 0.1 part of Iceland lichen extract, and deionized water to make up to 100 parts.

[0029] The sources of the Alpinia galanga leaf extract, Pyracantha fortuneana fruit extract, and Lichen truncatula extract in this example are the same as those in Example 1.

[0030] The preparation method of the hydrolyzed Ulva extract in this embodiment is as follows: S1: Wash the Ulva, dry it in vacuum at 50°C until the moisture content is ≤8%, grind it and pass it through a 100-mesh sieve to obtain Ulva powder; S2: The Ulva powder in step S1 was added to deionized water at a material-liquid ratio of 1 g:25 mL, and then 0.2% of the powder weight and 0.5% of the cellulase, protease and glucanase at a mass ratio of 2:1:1 were added. The mixture was enzymolyzed at a constant temperature of pH = 6.5 and 45°C for 3 h. After the enzymolysis was completed, the enzymes were inactivated to obtain an enzymolysis solution. S3: adding anhydrous ethanol 20 times the weight of the Ulva powder to the enzymatic hydrolyzate of step S2, stirring evenly, and then performing ultrasonic extraction at an ultrasonic power of 400 W, an ultrasonic time of 30 min, and an ultrasonic temperature of 60° C. The extract is first coarsely filtered through a 300-mesh filter cloth, and then finely filtered through a 0.5 μm microporous filter membrane to remove impurities to obtain a supernatant; S4: adding 0.5% by mass of mannitol as a freeze-drying protective agent to the supernatant of step S3 and then performing freeze-drying operation. The freeze-dried powder is the hydrolyzed Ulva extract.

[0031] Example 3 A naturally extracted anti-wrinkle and firming composition comprises, by weight, 50 parts of hydrolyzed ulva extract, 1.8 parts of Alpinia galanga leaf extract, 5 parts of Pyracantha fortuneana fruit extract, 0.2 parts of Iceland lichen extract, and deionized water to make up to 100 parts.

[0032] The sources of the Alpinia galanga leaf extract, Pyracantha fortuneana fruit extract, and Lichen truncatula extract in this example are the same as those in Example 1.

[0033] The preparation method of the hydrolyzed Ulva extract in this embodiment is as follows: S1: Wash the Ulva, vacuum dry at 45°C until the moisture content is ≤8%, grind and pass through an 80-mesh sieve to obtain Ulva powder; S2: The Ulva powder in step S1 was added to deionized water at a material-liquid ratio of 1 g:20 mL, and then 0.2% of the powder weight and 0.5% of the cellulase, protease and glucanase at a mass ratio of 2:1:1 were added. The mixture was enzymolyzed at a constant temperature of pH 6.5 and 42° C. for 2.5 h. After the enzymolysis was completed, the enzymes were inactivated to obtain an enzymolysis solution. S3: adding anhydrous ethanol 17 times the weight of the Ulva powder to the enzymatic hydrolyzate of step S2, stirring evenly, and then performing ultrasonic extraction at an ultrasonic power of 350 W, an ultrasonic time of 35 min, and an ultrasonic temperature of 50° C. The extract is first coarsely filtered through a 250-mesh filter cloth, and then finely filtered through a 0.45 μm microporous filter membrane to remove impurities to obtain a supernatant; S4: adding 0.45% by mass of mannitol as a freeze-drying protective agent to the supernatant of step S3 and then performing freeze-drying operation. The freeze-dried powder is the hydrolyzed Ulva extract.

[0034] On the basis of Example 2, the anti-wrinkle and firming compositions of Comparative Examples 1-4 were prepared by replacing the natural extracts of the same type.

[0035] Comparative Example 1 A naturally extracted anti-wrinkle and firming composition, which is different from Example 2 in that 2 parts by weight of seabuckthorn fruit extract is used to replace the original pyracantha fruit extract.

[0036] Sea buckthorn fruit extract and Pyracantha fruit extract are both fruit extracts, containing polyphenols (flavonoids) and vitamins (vitamin C). Both belong to the same type of natural extracts.

[0037] The seabuckthorn fruit extract of this comparative example was purchased from Shanxi Wutaishan Seabuckthorn Products Co., Ltd.

[0038] Comparative Example 2 A naturally extracted anti-wrinkle and firming composition, which differs from Example 2 in that 1 part by weight of rosemary leaf extract and 0.1 part by weight of calendula flower extract are used to replace the original Alpinia officinalis leaf extract and the original Iceland lichen extract.

[0039] The functional substances of rosemary leaf extract and Alpinia galanga leaf extract are volatile oils and flavonoids, and both belong to the same type of natural extracts.

[0040] The functional substances of calendula officinalis extract and Icelandic lichen extract are organic acids and polysaccharides, and both belong to the same type of natural extracts.

[0041] The rosemary leaf extract and calendula officinalis extract in this comparative example were purchased from Shaanxi Sinuote Biotechnology Co., Ltd.

[0042] Comparative Example 3 A naturally extracted anti-wrinkle and firming composition, which differs from Example 2 in that 20 parts by weight of brown algae extract, 2 parts by weight of pomegranate peel extract and 0.1 parts by weight of usnea extract are used to replace the original hydrolyzed ulva extract, original pyracantha fruit extract and original Icelandic lichen extract.

[0043] Brown algae extract and hydrolyzed ulva extract are both marine algae extracts, and their functional substances are sulfated polysaccharides and amino acids. Both belong to the same type of natural extracts.

[0044] Both pomegranate peel extract and Pyracantha fruit extract contain polyphenols. The vitamin C in Pyracantha fruit and the tannin in pomegranate peel are plant secondary metabolites, and both belong to the same type of natural extracts.

[0045] Both Usnea extract and Icelandic lichen extract are lichen extracts, and their functional substances are organic acids and polysaccharides. Both belong to the same type of natural extracts.

[0046] The brown algae extract, pomegranate peel extract and usnea extract in this comparative example were purchased from Xi'an Cuiyuan Biotechnology Co., Ltd.

[0047] Comparative Example 4 A naturally extracted anti-wrinkle and firming composition, which differs from Example 2 in that 20 parts by weight of spirulina extract, 1 part by weight of chamomile extract, 2 parts by weight of grapefruit extract, and 0.1 parts by weight of reindeer lichen extract are used to replace the original hydrolyzed Ulva extract, original Alpinia glabra leaf extract, original Pyracantha fortuneana fruit extract, and original Iceland lichen extract.

[0048] Spirulina extract and hydrolyzed Ulva extract are both marine algae extracts, and their functional substances are sulfated polysaccharides and amino acids. Both belong to the same type of natural extracts.

[0049] The functional substances of chamomile extract and Alpinia galanga leaf extract are volatile oils and flavonoids, and both belong to the same type of natural extracts.

[0050] Grapefruit extract and Pyracantha fruit extract both contain polyphenols and vitamins, and both belong to the same type of natural extracts.

[0051] Reindeer lichen extract and Icelandic lichen extract are both lichen extracts, and their functional substances are organic acids and polysaccharides. Both belong to the same type of natural extracts.

[0052] The spirulina extract in this comparative example was purchased from Zhejiang Binmei Biotechnology Co., Ltd.; the chamomile extract was purchased from Changsha Shanghe Biotechnology Co., Ltd.; the grapefruit extract was purchased from Zhejiang Baihui Biotechnology Co., Ltd.; and the reindeer lichen extract was purchased from Ningxia Vanilla Biotechnology Co., Ltd.

[0053] In order to further verify the performance of the present invention, comparative examples 5-6 of anti-wrinkle and firming compositions with chemical ingredients and comparative examples 7-8 of anti-wrinkle and firming compositions with plant ingredients were set.

[0054] Comparative Example 5 A chemical anti-wrinkle and firming composition comprises, by weight, 10 parts of hyaluronic acid, 5 parts of ceramide NP, 5 parts of ascorbyl glucoside, and deionized water to make up to 100 parts.

[0055] The hyaluronic acid in this comparative example was purchased from Bloomage Biotechnology Co., Ltd.; ceramide NP was purchased from Shanghai Jahwa United Co., Ltd.; and ascorbyl glucoside was purchased from Guangzhou Tianci High-tech Materials Co., Ltd.

[0056] Comparative Example 6 A chemical anti-wrinkle and firming composition comprises, by weight, 10 parts of bosaicin, 6 parts of dipeptide-1, 4 parts of retinol, and deionized water to make up to 100 parts.

[0057] The bosaicin, dipeptide-1 and retinol in this comparative example were purchased from Chengdu Yunxi Chemical Co., Ltd.

[0058] This comparative example Comparative Example 7 A plant-based anti-wrinkle and firming composition comprises, by weight, 12 parts of grape leaf extract, 5 parts of sunflower seed oil, 2 parts of African neem bark extract, 1 part of peony root extract, and deionized water to make up to 100 parts.

[0059] Grape leaf extract of this comparative example was purchased from Shaanxi Angsheng Biopharmaceutical Technology Co., Ltd.; sunflower seed oil was purchased from Yihai Kerry Golden Dragon Fish Cereals and Oils Food Co., Ltd.; African neem bark extract was purchased from BASF (Guangzhou) Cosmetics Co., Ltd.; and peony root extract was purchased from Xi'an Green Sky Biotechnology Co., Ltd. Comparative Example 8 A plant-based anti-wrinkle and firming composition comprises, by weight, 8 parts of Ganoderma lucidum extract, 2 parts of Centella asiatica extract, 2 parts of Polygonum cuspidatum root extract, 2 parts of Scutellaria baicalensis root extract, 2 parts of Portulaca oleracea extract, 2 parts of Panax ginseng root extract, 2 parts of Sophora flavescens root extract, and deionized water to make up to 100 parts.

[0060] The Ganoderma lucidum extract, Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Portulaca oleracea extract, Panax ginseng root extract, and Sophora flavescens root extract in this comparative example were all purchased from Shaanxi Sinuote Biotechnology Co., Ltd.

[0061] In order to better verify the effects of the above ingredients in practical applications of cosmetics, Verification Examples 1-3 and Verification Comparative Examples 1-8 were set up based on the original anti-wrinkle and firming compositions of Examples 1-3 and Comparative Examples 1-8.

[0062] Verification Examples 1-3 and Comparative Verification Examples 1-8 are aqueous solutions with a concentration of 1 wt % of the anti-wrinkle and firming composition.

[0063] Performance Testing 1. Safety test (1) Human skin patch test Refer to the human skin patch test in the 2022 edition of the "Safety Technical Specifications for Cosmetics" to test whether the samples in Verification Examples 1-3 will cause skin irritation or allergic reactions. The specific experimental steps are as follows: (1) 90 female subjects aged 18-60 were selected and divided equally into three groups, corresponding to samples 1-3 of validation cases, respectively. All subjects had no history of severe skin diseases, allergies, or immune diseases. (2) The selected area shall not exceed 50mm 2 Qualified patch test equipment with a depth of approximately 1mm was used. 0.02 mL of the sample from Examples 1-3 above was placed into the patch test chamber. The control well served as a blank control (no substance was placed). The sample-filled patch tester was applied to the healthy skin on both sides of the subject's spine using hypoallergenic tape. Gently press the patch evenly onto the skin with the palm of your hand for 24 hours. Skin reactions were observed 30 minutes after removal (after the indentation disappeared), 24 hours, and 48 hours after removal, and the results were recorded. The results are shown in Table 3 below, and the specific criteria for the results are shown in Table 4 below.

[0064] Table 1 Human skin patch test results Table 2 Specific standards for reaction results Reaction level Symptom description 0 No response 1+ Erythema, infiltration, papules 2+ Erythema, infiltration, papules, blisters 3+ Severe erythema, bullae, and ulcers As shown in Tables 1 and 2, all test subjects experienced a grade 0 reaction, meaning no allergic reactions occurred. This demonstrates that the anti-wrinkle and firming composition of the present invention is mildly irritating to the skin and exhibits good safety in practical applications.

[0065] (2) In accordance with T / SHRH008-2018 “Cosmetics - In Vitro Skin Irritation Test (SIT)”, in vitro skin irritation tests were performed on Verification Examples 1-3 and Comparative Examples 1-8. Each sample was tested three times, and the average of the three test results was taken as the final result. The results are detailed in Table 3.

[0066] Among them, an average relative tissue activity value of ≥50% indicates non-irritation, and an average relative tissue activity value of <50% indicates irritation.

[0067] Table 3 In vitro skin irritation test results As shown in Table 3, the anti-wrinkle and firming compositions of Examples 1-3 of the present invention are all non-irritating, indicating that the anti-wrinkle and firming compositions of the present invention are mild in effect and highly safe.

[0068] Furthermore, in Comparative Examples 1-4, which used similar substances, Comparative Examples 1 and 2 were non-irritating, while Comparative Examples 2 and 3 were irritating, but their irritation was slightly less than that of the chemical anti-wrinkle and firming compositions in Comparative Examples 5 and 6. Comparative Examples 7 and 8, which used botanical anti-wrinkle and firming compositions, were also non-irritating, mild, and safe.

[0069] 2. Efficacy test subjects: 330 volunteers were selected as subjects, aged 18-45 years old, all female, and divided into 11 groups, 30 people in each group. All subjects had no serious skin diseases, allergies or immune diseases, and met the subject criteria.

[0070] Testing Method: 30 minutes after cleansing the subject's face, wrinkle images of the periocular area were captured using the PRIMOS-CR rapid 3D skin imaging system. Target wrinkle analysis locations were determined, and the volume and area of ​​canthus and subocular wrinkles were determined using Primos 5.8 analysis software. Ultrasound scanning of the cheek skin was performed using the UC22 skin ultrasound tester, and dermal thickness and density were analyzed using analysis software. A Visioscan VC20plus was used to image and analyze the skin smoothness SEsm value at the test site. The Cutometer skin elasticity probe was used to measure the skin R0, R2, and F4 values ​​at the test site. These values ​​served as baseline values ​​(D0). Samples were then distributed and instructions for use were provided. The same indicators were tested on the 28th day after product use (D28). Test parameters are detailed in Table 4.

[0071] Table 4 Test parameters and parameter descriptions The test results are the rate of change of the test results of each parameter of each subject on D28 relative to the test results on D0. The average of the test results of each group of subjects is taken as the final result. The test results are detailed in Tables 5 and 6.

[0072] Table 5 Anti-wrinkle efficacy test results Table 6 Firming efficacy test results The above results all had significant differences.

[0073] From the test results in Table 5 and Table 6, it can be seen that Verification Examples 1-3 all have excellent anti-wrinkle and firming effects, indicating that the naturally extracted anti-wrinkle and firming composition of the present invention does have anti-wrinkle and firming effects.

[0074] Comparative Examples 1-4 were prepared by replacing the anti-wrinkle and firming composition of Example 2 with similar substances. As shown in Tables 5 and 6, Comparative Example 1 exhibited weak anti-wrinkle and firming effects, Comparative Example 2 exhibited good anti-wrinkle and weaker firming effects, Comparative Example 3 exhibited weaker anti-wrinkle and good firming effects, and Comparative Example 4 exhibited weaker anti-wrinkle and weaker firming effects. Combined with the irritation test results in Table 3, it can be seen that the composition of the present invention, through the synergistic effect of the four natural extracts, achieves a balance between mildness, anti-wrinkle, and firming effects.

[0075] The applicant further investigated the mechanism of action of the anti-wrinkle and firming composition of the present invention and discovered that it achieves its anti-wrinkle and firming effects through a multi-pathway, multi-layered interlocking mechanism. Ulva polysaccharide from hydrolyzed ulvae and Icelandic lichen polysaccharide form a "water-oil dual-phase moisturizing network." The former locks in moisture and promotes ingredient penetration, while the latter repairs the barrier and reduces water loss, collectively improving dryness-related fine lines. Vitamin C from Pyracantha fruit and flavonoids from Alpinia galanga leaves form an "antioxidant cascade." The former directly promotes collagen synthesis, while the latter inhibits collagen degradation by inflammatory factors, synergistically enhancing fibroblast activity. Icelandic lichen activates type III collagen repair, forming a dual "dermal support-elastic fiber" network with type I collagen promoted by Pyracantha fruit and Alpinia galanga leaves, increasing collagen fiber density. The synergistic effect of these four enhances the anti-wrinkle and firming effects.

[0076] Verification Comparative Examples 5-6 are chemical anti-wrinkle and firming compositions. Combined with the efficacy test results of Verification Example 2, it can be seen that the anti-wrinkle and firming compositions of the present invention have anti-wrinkle and firming effects similar to those of chemical compositions, while solving the shortcoming of high irritation of chemical anti-wrinkle and firming compositions.

[0077] Verification Comparative Examples 7-8 are anti-wrinkle and firming compositions containing plant ingredients. Combined with the efficacy test results of Verification Example 2, it can be seen that the anti-wrinkle and firming compositions of the present invention retain the advantage of mild effects of plant ingredients, while solving the disadvantage of weak anti-wrinkle and firming efficacy of plant ingredients.

[0078] To better utilize the anti-wrinkle and firming efficacy of the anti-wrinkle and firming composition of the present invention, the anti-wrinkle and firming composition of the present invention can be combined with one or more of a solvent, an emulsifier, an emollient, a humectant, a thickener, an antioxidant, a preservative, and a fragrance, as needed, to prepare an anti-wrinkle and firming cosmetic. The anti-wrinkle and firming cosmetic can be a serum, an essential oil, a cream, an emulsion, or an eye cream.

[0079] The solvent is one or more of water, butylene glycol and propylene glycol; The emulsifier is one or more of glyceryl stearate, polysorbate 80 and lecithin; The emollient is one or more of squalane, jojoba oil and shea butter; The moisturizing agent is one or more of glycerin, hyaluronic acid and panthenol; The thickener is one or more of carbomer, xanthan gum and hydroxyethyl cellulose; The antioxidant is one or more of vitamin E, tea polyphenols and coenzyme Q10; The preservative is one or more of phenoxyethanol, chlorphenesin and potassium sorbate; The aromatic agent is one or more of rose essential oil, lavender essential oil and sweet orange essential oil.

[0080] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A naturally extracted anti-wrinkle and firming composition, characterized in that: The following parts by weight are included: 1-50 parts of hydrolyzed Ulva extract; 0.06-1.8 parts of Alpinia glabra leaf extract; 0.1-5 parts of Pyracantha fortuneana fruit extract; Iceland lichen extract 0.01-0.2 parts.

2. The naturally extracted anti-wrinkle and firming composition according to claim 1, Its characteristics are In, according to parts by weight: 20 parts of hydrolyzed Ulva extract; 1 part of Alpinia glabra leaf extract; 2 parts of Pyracantha fortuneana fruit extract; Iceland lichen extract 0.1 parts.

3. The naturally extracted anti-wrinkle and firming composition according to claim 1, characterized in that The preparation method of the hydrolyzed ulva extract is as follows: S1: Wash and dry the Ulva, then crush it and sieve it to obtain Ulva powder; S2: adding deionized water and biological enzymes to the Ulva powder in step S1 for 2-3 hours, and then inactivating the enzymes to obtain an enzymatic solution; in, The biological enzyme is a compound of cellulase, protease and glucanase; S3: adding anhydrous ethanol in an amount of 15-20 times the weight of the Ulva powder to the enzymatic hydrolyzate of step S2, performing ultrasonic extraction, and filtering the extract to obtain a supernatant; S4: freeze-drying the supernatant of step S3 to obtain a hydrolyzed Ulva extract.

4. Use of the naturally extracted anti-wrinkle and firming composition according to claim 1 in the preparation of cosmetics.

5. The use according to claim 4, characterized in that: The cosmetic is essence, essence oil, face cream, lotion or eye cream.

6. A cosmetic, characterized in that: The invention comprises the naturally extracted anti-wrinkle and firming composition according to claim 1.

7. The cosmetic according to claim 6, characterized in that: The cosmetic may further comprise one or more of a solvent, an emulsifier, an emollient, a moisturizer, a thickener, an antioxidant, a preservative and a fragrance.

8. The cosmetic according to claim 7, characterized in that: The solvent is one or more of water, butylene glycol and propylene glycol; The emulsifier is one or more of glyceryl stearate, polysorbate 80 and lecithin; The emollient is one or more of squalane, jojoba oil and shea butter; The moisturizing agent is one or more of glycerin, hyaluronic acid and panthenol; The thickener is one or more of carbomer, xanthan gum and hydroxyethyl cellulose; The antioxidant is one or more of vitamin E, tea polyphenols and coenzyme Q10; The preservative is one or more of phenoxyethanol, chlorphenesin and potassium sorbate; The aromatic agent is one or more of rose essential oil, lavender essential oil and sweet orange essential oil.

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