Anti-hair loss composition extracted by fermentation as well as fermentation method and application of anti-hair loss composition
The anti-hair loss composition extracted through fermentation, using a combination of specific plant raw materials and bacterial strains, solves the problem of poor effectiveness of existing anti-hair loss products, achieves significant anti-hair loss and soothing effects, and restores scalp health.
Patent Information
- Application Number
- CN202511043705.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-28
- Publication Date
- 2025-10-10
AI Technical Summary
The chemically synthesized ingredients in existing anti-hair loss products have side effects, and the anti-hair loss effects of natural plant extracts are limited, making it difficult to meet consumer demand.
The anti-hair loss composition is prepared by fermentation extraction using plant raw materials such as Platycladus orientalis leaves, Lithospermum erythrorhizon, Gallnut, Alpinia officinalis, Radix Ampelopsis, Sanguisorba officinalis, and Callicarpa spicata through complex fermentation with methylotrophic Bacillus and Portuguese Corynespora yeast. The active ingredients are extracted by combining specific proportions and process steps to exert a synergistic effect.
Significantly improves the anti-hair loss effect, significantly soothes the scalp, reduces stress-induced hair loss, repairs hair follicles, restores the scalp to a healthy state, and provides a long-lasting anti-hair loss effect.
Smart Images
Figure BDA0005521126770000081 
Figure BDA0005521126770000091
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the field of washing and care product raw materials, in particular to a fermented anti-hair loss composition, a fermentation method thereof and application thereof. BACKGROUND
[0002] At present, cosmetics for preventing hair loss on the market mostly use chemical synthetic ingredients such as minoxidil, etc. Although these ingredients can inhibit hair loss to some extent, long-term use may produce side effects, and the improvement effect on the scalp environment is limited, ultimately resulting in a general anti-hair loss effect. At the same time, with the increasing demand of consumers for natural and healthy products, some anti-hair loss products add plant extracts with certain anti-hair loss effect to improve the anti-hair loss effect. However, the anti-hair loss effect of the existing anti-hair loss products adding plant extracts is still general, which is difficult to meet the needs of consumers, and therefore there is room for improvement. SUMMARY
[0003] In order to further improve the anti-hair loss effect, the present application provides a fermented anti-hair loss composition, a fermentation method thereof and application thereof.
[0004] In a first aspect, the present application provides a fermentation method of a fermented anti-hair loss composition, which adopts the following technical solution: A fermentation method of a fermented anti-hair loss composition, comprising the following steps: Step 1), mixing, crushing and obtaining a composite plant raw material by mixing Platycladus orientalis, Radix Lithospermi, Chinese gall, processed ginger, processed white vine, Sanguisorba officinalis and Callicarpa bodinieri; Step 2), mixing the composite plant raw material with water, adding a fermentation bacterial solution for fermentation to obtain a fermentation mixture; Step 3), heating the fermentation mixture for inactivation, then performing cell lysis treatment to obtain a cell lysis mixture; Step 4), filtering the cell lysis mixture, taking the filtrate to obtain a composite extract; Step 5), concentrating and drying the composite extract to obtain a fermented anti-hair loss composition; The fermentation bacterial solution is obtained by culturing a composite fermentation bacterium in a liquid culture medium, and the OD value of the fermentation bacterial solution is greater than or equal to 1. The composite fermentation bacterium is a composite of methylotrophic Bacillus and Portuguese rod-shaped yeast.
[0005] By adopting the above technical solution, the fermented anti-hair loss composition obtained by fermenting the composite of Platycladus orientalis, Radix Lithospermi, Chinese gall, processed ginger, processed white vine, Sanguisorba officinalis and Callicarpa bodinieri as raw materials can bring about a remarkable anti-hair loss effect through the synergistic effect of the active ingredients of each raw material, thereby significantly improving the hair loss phenomenon and meeting the needs of consumers.
[0006] Through the combination of methylotrophic Bacillus and Portuguese Corynespora yeast, the fermented lysate and active ingredients produce a special compound effect, which further improves the anti-hair loss effect and can bring a significant soothing effect. It can repair the scalp and hair follicles well, restore the health of the scalp and hair follicles, reduce stress-induced hair loss caused by allergies due to scalp irritation, and make the anti-hair loss effect more significant and longer-lasting.
[0007] Preferably, in the step 1), the mass ratio of Platycladus orientalis leaf, Lithospermum erythrorhizon, Gallnut, processed Alpinia officinalis, processed Bletilla striata, Sanguisorba officinalis, and Callicarpa oleracea is 4-5:12-13:7-8:2-3:15-16:1-2:5-6.
[0008] By adopting the above technical scheme and specifically selecting the mass ratios of Platycladus orientalis leaves, Lithospermum erythrorhizon, Gallnut, Roasted Alpinia officinalis, Roasted Bletilla striata, Sanguisorba officinalis, and Callicarpa squarrosa, the active ingredients can be better combined in a specific ratio, thereby exerting a more significant synergistic effect and achieving a more significant anti-hair loss effect.
[0009] Preferably, in the composite fermentation bacteria, the mass ratio of methylotrophic Bacillus and Portuguese yeast is 1-2:7-8.
[0010] By adopting the above technical solution and specifically selecting the mass ratio of methylotrophic Bacillus and Portuguese yeast, the various components in the fermented lysate are better matched in a specific ratio, so that the lysate and the active ingredients cooperate with each other better, and the anti-hair loss and soothing effects are more significant.
[0011] Preferably, in step 2), the mass ratio of the composite plant raw material, water, and fermentation liquid is 46-53:92-159:4.6-10.6.
[0012] By adopting the above technical solution and specifically selecting the mass ratio of the composite plant raw material, water and fermentation liquid, a better fermentation effect is ensured, while the waste of the fermentation liquid is reduced, thereby having higher economic value.
[0013] Preferably, in step 2, after adding the fermentation broth, the temperature is maintained at 30-35° C. and fermented for 28-30 days.
[0014] By adopting the above technical solution and specifically selecting a constant temperature of 30-35°C and fermenting for 28-30 days, the fermentation effect is better and the fermentation is more complete, and the anti-hair loss and soothing effects of the anti-hair loss composition are better.
[0015] Preferably, in step 3), during inactivation, the fermentation mixture is heated to boiling, condensed and refluxed, and kept boiling for 2-3 hours.
[0016] By adopting the technical scheme, through boiling for 2-3 hours, inactivation can be more thorough, and further extraction can be achieved, so that the active ingredients in the composite plant raw material can be better extracted, thereby making the anti-hair loss composition have better anti-hair loss and soothing effects.
[0017] Preferably, in the step 3), after inactivation, lysis treatment is performed by adding lysozyme.
[0018] By adopting the technical scheme, lysis treatment is performed by specifically selecting lysozyme, the lysis effect is better, the process difficulty is lower, and the economic value is higher.
[0019] In a second aspect, the application provides a fermentation-extracted anti-hair loss composition, which adopts the following technical scheme: A fermentation-extracted anti-hair loss composition, which is prepared by the fermentation method of the fermentation-extracted anti-hair loss composition described above.
[0020] By adopting the technical scheme, the fermentation-extracted anti-hair loss composition has significant anti-hair loss and soothing effects, so that the effect of improving hair loss is better and more durable, and the needs of consumers are well met.
[0021] In a third aspect, the application provides an application of the fermentation-extracted anti-hair loss composition, which adopts the following technical scheme: an application of the fermentation-extracted anti-hair loss composition described above, characterized in that the fermentation-extracted anti-hair loss composition is added to a shampoo product to prepare a shampoo product with anti-hair loss effect.
[0022] By adopting the technical scheme, the prepared shampoo product can well and durably reduce hair loss and durably soothe the scalp, so that the scalp is more likely to recover.
[0023] In summary, the application has the following beneficial effects: 1. Since the application adopts Platycladus orientalis, Arnebia euchroma, Chinese gall, processed ginger, processed white vine, Sanguisorba officinalis and Fargesia spathacea as raw materials, and the fermentation-extracted anti-hair loss composition is obtained after fermentation, the active ingredients of the raw materials cooperate with each other to produce a synergistic effect, thereby bringing a more significant anti-hair loss effect. In addition, the combination of Bacillus methylotrophicus and Starmerella portuguese makes the lysis product after fermentation and the active ingredients produce a special combination effect, so that the anti-hair loss effect is further improved, and a significant soothing effect is brought, the scalp and hair follicles can be well repaired, the scalp and hair follicles can recover, the allergic reaction caused by the stimulation of the scalp can be reduced, the stress-induced hair loss phenomenon can be reduced, and the anti-hair loss effect is more significant and more durable.
[0024] 2, The quality ratio of Platycladus orientalis, Arnebia euchroma, Chinese gall, processed ginger, processed white vine, Sanguisorba officinalis, and Fagraea ceilanica is specifically selected in the application, so that the active ingredients are better matched in a specific ratio, and the synergistic effect is more remarkable, thereby achieving a more remarkable anti-hair loss effect.
[0025] 3, The quality ratio of Bacillus methylotrophicus and Starmerella portugalicola is specifically selected in the application, and the various components in the lysed product after fermentation are better matched in a specific ratio, so that the lysed product and the active ingredients are better matched, and the anti-hair loss and soothing effects are more remarkable. DETAILED DESCRIPTION
[0026] The application will be further described in detail below in combination with examples.
[0027] Example 1 A fermentation-extracted anti-hair loss composition, and the fermentation method comprises the following steps: Step 1), 4 kg of Platycladus orientalis, 12 kg of Arnebia euchroma, 7 kg of Chinese gall, 2 kg of processed ginger, 15 kg of processed white vine, 1 kg of Sanguisorba officinalis, and 5 kg of Fagraea ceilanica are mixed and crushed, and then passed through an 80-mesh sieve to obtain a composite plant raw material.
[0028] Step 2), 46 kg of the composite plant raw material and 92 kg of water are put into a reaction kettle, stirred at a speed of 90 r / min for 15 min, and then 4.6 kg of fermentation bacteria liquid is added, and the temperature is kept at 30°C for 28 days of fermentation to obtain a fermentation mixture.
[0029] Step 3), the fermentation mixture is heated to boiling, and the condensate is refluxed, and the boiling is kept for 2 h to complete the inactivation, then 9.2 g of lysozyme is added, and the stirring speed is 120 r / min, and the stirring time is 80 min to complete the lysis treatment, and a lysed mixture is obtained.
[0030] Step 4), the lysed mixture is filtered through filter cloth, and the filtrate is obtained to obtain a composite extract.
[0031] Step 5), the composite extract is concentrated by rotary evaporation at 0.01 MPa and 55°C, and after the paste is concentrated to a paste, the paste is dried by spraying to obtain a fermentation-extracted anti-hair loss composition.
[0032] Platycladus orientalis, Arnebia euchroma, Chinese gall, processed ginger, processed white vine, Sanguisorba officinalis, and Fagraea ceilanica are all dry products dried in the sun, and are purchased from Feng Yixiong Medicine Store in Qingping Traditional Chinese Medicine Professional Market, Liwan District, Guangzhou City.
[0033] Lysozyme is purchased from Sichuan Huanxu Biological Technology Co., Ltd.
[0034] The fermentation bacteria liquid is self-made, and the preparation method is as follows: Step 01), 100 kg of water, 4 kg of glucose, 2 kg of beef extract, 1 kg of peptone, 0.14 kg of sodium chloride, 0.04 kg of potassium dihydrogen phosphate, 0.04 kg of magnesium sulfate, and 0.4 kg of vitamin B1 were put into a stirred tank at a speed of 120 r / min for 20 min to obtain a liquid medium.
[0035] Step 02), the methylotrophic Bacillus and Portuguese clavispora were put into the liquid medium, and the temperature was kept at 30℃ until the OD value of the bacterial liquid was 1 to obtain the fermented bacterial liquid.
[0036] The glucose was purchased from Jinan Yuanlian Chemical Co., Ltd.
[0037] The beef extract was purchased from Jinan Jibin Chemical Co., Ltd.
[0038] The peptone was purchased from Hubei Haijia Biological Technology Co., Ltd.
[0039] The sodium chloride was purchased from Sichuan Puhe Chemical Co., Ltd.
[0040] The potassium dihydrogen phosphate was purchased from Weifang Huabo Chemical Co., Ltd.
[0041] The magnesium sulfate was purchased from Hebei Runbu Biological Technology Co., Ltd.
[0042] The vitamin B1 was purchased from Guangzhou Huayu Biological Technology Co., Ltd.
[0043] The methylotrophic Bacillus was purchased from Shanghai Collection Center of Biological Technology, and the strain number was SHBCC D53055.
[0044] The Portuguese clavispora was purchased from Beijing Baoe Bowei Biological Technology Co., Ltd., and the international number was ATCC 38533.
[0045] Example 2 A fermentation-extracted anti-falling composition, the fermentation method comprising the following steps: Step 1), 4.5 kg of biota leaves, 12.5 kg of geocrypta, 7.5 kg of gallnut, 2.5 kg of gunny ginger, 15.5 kg of gunny white vine, 1.5 kg of sanguisorba officinalis, and 5.5 kg of lonicera japonica were mixed, crushed, and passed through an 80-mesh sieve to obtain a composite plant raw material.
[0046] Step 2), 49.5 kg of the composite plant raw material and 123.75 kg of water were put into a reaction kettle at a speed of 90 r / min for 15 min, and then 7.4 kg of the fermented bacterial liquid was added, and the temperature was kept at 32.5℃ for 29 days to obtain a fermentation mixture.
[0047] Step 3), the fermentation mixture was heated to boiling, reflux condensation was kept boiling for 2.5h, and inactivation was completed, then 15.2g lysozyme was added, stirring at 120r / min for 90min, and the lysis treatment was completed to obtain a lysis mixture.
[0048] Step 4), the lysis mixture was filtered through filter cloth, and the filtrate was obtained to obtain a composite extract.
[0049] Step 5), the composite extract was concentrated at 0.01MPa and 55℃ by rotary evaporation, and after the paste was concentrated, the paste was spray-dried to obtain a fermentation-extracted anti-falling composition.
[0050] Platycladus orientalis, geoclemys, gallnut, pao Liang ginger, pao Bai Teng, willow, and purple pearl are all dried products, and are purchased from Feng Yixiong Medicine Store in Qingping Traditional Chinese Medicine Professional Market in Liwan District, Guangzhou.
[0051] Lysozyme was purchased from Sichuan Huanxi Biological Technology Co., Ltd.
[0052] The fermentation broth was self-made, and the preparation method was as follows: Step 01), 100kg of water, 4.5kg of glucose, 2.5kg of beef extract, 1.5kg of peptone, 0.15kg of sodium chloride, 0.05kg of potassium dihydrogen phosphate, 0.05kg of magnesium sulfate, and 0.5kg of vitamin B1 were put into a stirring kettle, stirring at 120r / min for 20min to obtain a liquid medium.
[0053] Step 02), the methylotrophic Bacillus was put into the liquid medium, and the temperature was kept at 32.5℃ until the OD value of the bacterial liquid was 1 to obtain a fermentation broth.
[0054] Glucose was purchased from Jinan Yuanlian Chemical Co., Ltd.
[0055] Beef extract was purchased from Jinan Jibin Chemical Co., Ltd.
[0056] Peptone was purchased from Hubei Haijia Biological Technology Co., Ltd.
[0057] Sodium chloride was purchased from Sichuan Puhe Chemical Co., Ltd.
[0058] Potassium dihydrogen phosphate was purchased from Weifang Huabo Chemical Co., Ltd.
[0059] Magnesium sulfate was purchased from Hebei Runbu Biological Technology Co., Ltd. Vitamin B1 was purchased from Guangzhou Huayu Biological Technology Co., Ltd.
[0060] Methylotrophic Bacillus was purchased from Shanghai Collection and Biological Technology Center, and the strain number was SHBCC D53055.
[0061] The yeast Corynespora lucidum was purchased from Beijing Biobo Biotechnology Co., Ltd. with the international number: ATCC 38533.
[0062] Example 3 A hair loss prevention composition extracted by fermentation, wherein the fermentation method comprises the following steps: Step 1) 5 kg of Platycladus orientalis leaves, 13 kg of Lithospermum officinale, 8 kg of Gallnut, 3 kg of processed Alpinia officinalis, 16 kg of processed Bletilla striata, 2 kg of Sanguisorba officinalis, and 6 kg of Callicarpa oleifera were mixed and crushed, and passed through an 80-mesh sieve to obtain a composite plant raw material.
[0063] Step 2) 53 kg of composite plant material and 159 kg of water were put into a reactor at a speed of 90 r / min and stirred for 15 minutes to mix evenly. Then 10.6 kg of fermentation bacteria liquid was added and the mixture was fermented at a constant temperature of 35° C. for 30 days to obtain a fermentation mixture.
[0064] Step 3) The fermentation mixture was heated to boiling, condensed and refluxed, and kept boiling for 3 hours to complete inactivation, and then 21.2 g of lysozyme was added, the speed was 120 r / min, and the mixture was stirred for 100 minutes to complete the lysis treatment to obtain a lysis mixture.
[0065] Step 4), filtering the lysed mixture through filter cloth, taking the filtrate to obtain a composite extract.
[0066] Step 5) The composite extract is concentrated by rotary evaporation at 0.01 MPa and 55° C. to a paste, and the paste is powdered and dried to obtain a fermented and extracted anti-hair loss composition.
[0067] Platycladus orientalis leaves, lithospermum erythrorhizon, gallnut, roasted galangal, roasted white peony root, sanguisorba officinalis, and calliandra are all sun-dried products, and were purchased from Feng Yixiong's herbal medicine store in Qingping Chinese herbal medicine market in Liwan District, Guangzhou.
[0068] Lysozyme was purchased from Sichuan Huanxu Biotechnology Co., Ltd.
[0069] The fermentation broth was homemade and the preparation method was as follows: Step 01) 100 kg of water, 5 kg of glucose, 3 kg of beef extract, 2 kg of peptone, 0.16 kg of sodium chloride, 0.06 kg of potassium dihydrogen phosphate, 0.06 kg of magnesium sulfate, and 0.6 kg of vitamin B1 were added to a stirred tank at a speed of 120 r / min and stirred for 20 min to obtain a liquid culture medium.
[0070] Step 02) methylotrophic Bacillus and Portuguese yeast were added to a liquid culture medium, kept at a constant temperature of 35° C., and cultured until the OD value of the bacterial liquid was 1, thereby obtaining a fermentation bacterial liquid.
[0071] Glucose was purchased from Jinan Yuanlian Chemical Co., Ltd.
[0072] The beef paste was purchased from Jinan Jibin Chemical Co., Ltd.
[0073] Peptone was purchased from Hubei Haijia Biotechnology Co., Ltd.
[0074] Sodium chloride was purchased from Sichuan Puhe Chemical Co., Ltd.
[0075] Potassium dihydrogen phosphate was purchased from Weifang Huabo Chemical Co., Ltd.
[0076] Magnesium sulfate was purchased from Hebei Runbu Biotechnology Co., Ltd. Vitamin B1 was purchased from Guangzhou Huayu Biotechnology Co., Ltd.
[0077] Methylotrophic Bacillus was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D53055.
[0078] The yeast Corynespora lucidum was purchased from Beijing Biobo Biotechnology Co., Ltd. with the international number: ATCC 38533.
[0079] Comparative Example 1 A fermented and extracted anti-hair loss composition, compared with Example 2, differs only in that: Use an equal amount of Panax notoginseng to replace the Platycladus orientalis leaves.
[0080] Panax notoginseng was sun-dried and purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.
[0081] Comparative Example 2 A fermented and extracted anti-hair loss composition, compared with Example 2, differs only in that: Use Sophora flavescens to replace Lithospermum erythrorhizon in equal amounts.
[0082] Sophora flavescens was sun-dried and purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.
[0083] Comparative Example 3 A fermented and extracted anti-hair loss composition, compared with Example 2, differs only in that: An equal amount of Polygonum multiflorum was used to replace Gallnut.
[0084] Polygonum multiflorum was sun-dried and purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.
[0085] Comparative Example 4 A fermented and extracted anti-hair loss composition, compared with Example 2, differs only in that: Use an equal amount of mulberry leaves to replace the roasted galangal.
[0086] The mulberry leaves were sun-dried and purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.
[0087] Comparative Example 5 A fermented and extracted anti-hair loss composition, compared with Example 2, differs only in that: Use an equal amount of black sesame seeds to replace the roasted white peony root.
[0088] Black sesame seeds were sun-dried and purchased from Bozhou Deng Dawei Pharmaceutical Co., Ltd.
[0089] Comparative Example 6 A fermented and extracted anti-hair loss composition, compared with Example 2, differs only in that: Replace the burnet with an equal amount of rosemary.
[0090] Rosemary was sun-dried and purchased from Bozhou Huozhengtang Pharmaceutical Co., Ltd.
[0091] Comparative Example 7 A fermented and extracted anti-hair loss composition, compared with Example 2, differs only in that: Use an equal amount of angelica to replace purple beads.
[0092] Angelica sinensis was sun-dried and purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.
[0093] Comparative Example 8 A fermented and extracted anti-hair loss composition, compared with Example 2, differs only in that: Bacillus subtilis was used in equal amounts to replace the methylotrophic Bacillus.
[0094] Bacillus subtilis was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd. with the international number: ATCC 21951.
[0095] Comparative Example 9 A fermented and extracted anti-hair loss composition, compared with Example 2, differs only in that: Kluyveromyces marxianus was used to replace the Portuguese coryneform yeast in equal amounts.
[0096] Kluyveromyces marxianus was purchased from Beijing Biobo Biotechnology Co., Ltd. with the international number: ATCC46537.
[0097] Comparative Example 10 A fermented and extracted anti-hair loss composition, compared with Example 2, differs only in that: Use an equal amount of Panax notoginseng to replace Platycladus orientalis leaves, use an equal amount of Sophora flavescens to replace Lithospermum erythrorhizon, use an equal amount of Polygonum multiflorum to replace Gallnut, use an equal amount of mulberry leaves to replace Rhizoma Alpiniae Alpiniae, use an equal amount of black sesame to replace Rhizoma Bletillae, use an equal amount of Rosemary to replace Sanguisorba officinalis, use an equal amount of Angelica sinensis to replace Callicarpa ovata, use an equal amount of Bacillus subtilis to replace Bacillus methylotrophicus, and use an equal amount of Kluyveromyces marxianus to replace Klebsiella pneumoniae.
[0098] Panax notoginseng was sun-dried and purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.
[0099] Sophora flavescens is a dried product, purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.
[0100] Radix Polygoni Multiflori is a dried product, purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.
[0101] Mulberry leaves are dried products, purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.
[0102] Black sesame is a dried product, purchased from Bozhou Deng Dawei Pharmaceutical Co., Ltd.
[0103] Rosmarinus officinalis is a dried product, purchased from Bozhou Huozhengtang Pharmaceutical Co., Ltd.
[0104] Angelica sinensis is a dried product, purchased from Bozhou Fuyongtang Pharmaceutical Co., Ltd.
[0105] Bacillus subtilis was purchased from Beijing Baoerbowei Biotechnology Co., Ltd., and the international number is ATCC 21951.
[0106] Kluyveromyces marxianus was purchased from Beijing Baoerbowei Biotechnology Co., Ltd., and the international number is ATCC 46537.
[0107] Experiment 1 A number of volunteers were recruited, all male, all with hair loss, and each group of 10 volunteers used the hair loss prevention composition prepared from the fermented extract of each example and the comparative example to prepare the test shampoo, and the experiment lasted for 30 days. The volunteers washed their hair once every two days, and at the first washing, the wastewater generated by washing the hair was collected, and the hair loss was filtered and recorded. The average value of each group was taken to obtain the initial hair loss amount.
[0108] On the 30th day, the wastewater generated by washing the hair was collected, and the hair loss was filtered and recorded. The average value of each group was taken to obtain the hair loss amount on the 30th day.
[0109] Preparation of the test shampoo: 74g of water, disodium EDTA, 8g of sodium laureth sulfate, 14g of ammonium lauryl ether sulfate, 3g of cocamide MEA, and 1g of the fermented extract of the hair loss prevention composition were mixed uniformly to obtain the test shampoo.
[0110] Experiment 2 According to T / JSRH 003-2023 "Cosmetic raw material anti-allergy activity test hyaluronidase inhibition method", the hyaluronidase inhibition rate (%) of the test sample prepared from the fermented extract of the hair loss prevention composition of each example and the comparative example was detected.
[0111] Test sample: 1g of the composition with soothing and repairing effect, 99g of deionized water, mixed uniformly.
[0112] The specific experimental data of Experiment 1-2 are shown in Table 1.
[0113] Table 1 According to the comparison of the data of each example and the comparative example in Table 1, the anti-hair loss effect of each example is significantly better than that of each comparative example, and the soothing effect of each example is also significantly better than that of each comparative example. It can be seen that, in each example, the compound plant raw material composed of Platycladus orientalis, Arnebia euchroma, Chinese gall, processed ginger, processed white vine, Sanguisorba officinalis and Fargesia muriel is fermented by Bacillus methylotrophicus and Starmerella portugaliensis, and the fermented anti-hair loss composition obtained by extraction has special compound effect of various active ingredients and cell lysates, so that the fermented anti-hair loss composition can significantly reduce the hair loss phenomenon, soothe the scalp, make the scalp recover better, reduce the stress hair loss phenomenon caused by scalp allergy, and make the anti-hair loss effect better and more durable.
[0114] The specific embodiments are only an explanation of the present application, and are not a limitation of the present application. Those skilled in the art can make modifications to the embodiments without creative contribution after reading the present specification, and the modifications are protected by the patent law as long as they are within the scope of the claims of the present application.
Claims
1. A fermentation method for producing a hair loss prevention composition by fermentation extraction, characterized in that: The following steps are involved: Step 1) mixing and crushing Platycladus orientalis leaves, Lithospermum officinale, Gallnut, processed Alpinia officinalis, processed Bletilla striata, Sanguisorba officinalis, and Callicarpa ovata to obtain a composite plant raw material; Step 2), mixing the composite plant material with water, adding a fermentation solution and fermenting to obtain a fermentation mixture; Step 3), heating the fermentation mixture to inactivate it, and then performing a lysis treatment to obtain a lysis mixture; Step 4), filtering the lysed mixture and collecting the filtrate to obtain a composite extract; Step 5), concentrating and drying the composite extract to obtain a fermented and extracted anti-hair loss composition; The fermentation broth is prepared by culturing the composite fermentation bacteria in a liquid culture medium, and the OD value of the fermentation broth is ≥1; The composite fermentation bacteria is a combination of methylotrophic Bacillus and Portuguese Corynespora yeast.
2. The fermentation method of the anti-hair loss composition obtained by fermentation extraction according to claim 1, wherein: In the step 1), the mass ratio of Platycladus orientalis leaf, Lithospermum erythrorhizon, Gallnut, processed Alpinia officinalis, processed Bletilla striata, Sanguisorba officinalis, and Callicarpa oleracea is 4-5:12-13:7-8:2-3:15-16:1-2:5-6.
3. The fermentation method of the anti-hair loss composition obtained by fermentation extraction according to claim 2, wherein: In the composite fermentation bacteria, the mass ratio of methylotrophic Bacillus and Portuguese Corynespora is 1-2:7-8.
4. The fermentation method of the anti-hair loss composition obtained by fermentation extraction according to claim 3, characterized in that: In the step 2), the mass ratio of the composite plant raw material, water, and fermentation liquid is 46-53:92-159:4.6-10.
6.
5. The fermentation method of the anti-hair loss composition obtained by fermentation extraction according to claim 4, characterized in that: In the step 2, after adding the fermentation liquid, the temperature is kept at 30-35° C. and fermented for 28-30 days.
6. The fermentation method of the anti-hair loss composition obtained by fermentation extraction according to claim 1, characterized in that: In the step 3), during the inactivation, the fermentation mixture is heated to boiling, condensed and refluxed, and kept boiling for 2-3 hours.
7. The fermentation method of the anti-hair loss composition obtained by fermentation extraction according to claim 6, characterized in that: In the step 3), after inactivation, lysozyme is added to perform cell lysis.
8. A fermented and extracted anti-hair loss composition, characterized in that: The anti-hair loss composition obtained by fermentation extraction is prepared by the fermentation method of the anti-hair loss composition obtained by fermentation extraction according to any one of claims 1 to 7.
9. An application of the anti-hair loss composition obtained by fermentation extraction according to claim 8, characterized in that: The anti-hair loss composition extracted by fermentation is added into shampoo products to prepare shampoo products with anti-hair loss efficacy.