Composition containing camellia nitidissima extract and application

Abstract

The invention provides a composition containing a camellia nitidissima extract and application. The composition is prepared from the following components in parts by mass: 0.01 to 3 parts of camellia nitidissima flower extract, 0.01 to 5 parts of camellia nitidissima leaf extract, 0.01 to 1 part of camellia nitidissima essential oil, 0.05 to 2 parts of radix ophiopogonis root extract and 0.01 to 2 parts of dendrobium officinale stem extract. The components of the composition containing the camellia nitidissima extract are synergistic to realize multi-dimensional synergistic repair of a skin barrier: the camellia nitidissima essential oil promotes keratinocyte proliferation and skin renewal, and opens transdermal channels for other components at the same time; the camellia nitidissima flower extract and the camellia nitidissima leaf extract are combined, so that oxidative damage and inflammation are relieved, and cuticle is stabilized; the radix ophiopogonis root extract repairs a skin barrier and provides a storage space for NMF generated by the dendrobium officinale stem extract, and silk protein of the dendrobium officinale stem extract promotes formation of a breathable biological membrane and generates NMF, so that a support is provided for improvement of the skin barrier by the radix ophiopogonis root extract.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and particularly relates to a composition containing a camellia chrysantha extract and an application thereof. Background Art

[0002] The integrity of the skin's barrier function relies on a dynamic balance between the physical barrier (the lipid bilayer of the stratum corneum and the natural moisturizing factor derived from filaggrin), the chemical barrier (the antioxidant network and weakly acidic environment), and the immune barrier (inflammatory factor regulation and microbial balance). Currently, most products on the market are limited to repairing a single barrier mechanism and utilize large-molecule active substances such as polysaccharides. These ingredients have poor transdermal efficacy and can provide short-term hydration, but they cannot activate the expression of barrier-related proteins, which can easily lead to a rapid decline in skin barrier function after discontinuation of use.

[0003] Although studies have shown that Camellia chrysantha extract can be used in restorative skin care products, most of the current Camellia chrysantha-related skin care products only use crude extractions from a single part and apply a single active level. For example, only polyphenol components of Camellia chrysantha flowers are extracted for antioxidant effects, or only Camellia chrysantha tea leaf extracts are prepared for anti-inflammatory effects, etc., ignoring the differences in chemical composition of different organs such as flowers, leaves, and fruits / seeds, and lacking research on the synergistic effects of different tissue structures of Camellia chrysantha in moisturizing and barrier repair.

[0004] Therefore, it is of great value to develop a multi-dimensional and long-lasting barrier repair product. Summary of the Invention

[0005] To address the above-mentioned issues, the present invention provides a composition containing a Camellia chrysantha extract and its application. The composition synergistically repairs the barrier in multiple dimensions, and the repair effect is long-lasting and not prone to rebound after discontinuation of use.

[0006] On the one hand, the present invention provides the above-mentioned composition containing Camellia chrysantha extract, which includes the following components in parts by mass: 0.01 to 3 parts of Camellia chrysantha flower extract, 0.01 to 5 parts of Camellia chrysantha tea leaf extract, 0.01 to 1 part of Camellia chrysantha essential oil, 0.05 to 2 parts of Ophiopogon japonicus root extract, and 0.01 to 2 parts of Dendrobium officinale stem extract.

[0007] The components of the composition containing camellia chrysantha extract of the present invention work together to achieve multi-dimensional synergistic repair of the skin barrier: camellia chrysantha essential oil is a volatile oil present in camellia chrysantha flowers, camellia tea leaves and camellia chrysantha fruits. It is volatile at room temperature and is mainly composed of simple compounds with small molecular weight. It contains a large amount of linalool. Linalool has high penetration efficiency, promotes keratinocyte proliferation and skin renewal, and opens transdermal channels for other components. Camellia chrysantha extract contains tea polyphenols and tea polysaccharides, which have the ability to neutralize free radicals and reduce oxidative damage. It also contains various amino acids such as theanine, which can effectively promote skin water absorption and moisturizing, and relieve problems such as dry skin and tightness. Camellia chrysantha extract is rich in various functional active ingredients such as saponins, flavonoids, polyphenols, amino acids, etc. Epigallocatechin gallate and EGCG in flavonoid glycosides and polyphenols can reduce the degree of cell damage degree, inhibiting inflammatory factors, reducing hyaluronidase gene expression, and stabilizing the stratum corneum, that is, the combination of Camellia chrysantha flower extract and Camellia chrysantha tea leaf extract can reduce oxidative damage and inflammation and stabilize the barrier; the polyfructose in Ophiopogon japonicus root extract can strengthen the adhesion between keratinocytes, improve the skin barrier, and promote the synthesis and reserve of the skin's natural moisturizing factor (NMF); Dendrobium officinale stem extract forms a breathable biofilm on the skin surface with its highly water-absorbing polysaccharide, significantly reduces water loss (TEWL), upregulates the expression of filaggrin (FLG), and continuously generates NMF, solving the pain point of rapid decline in the effect of the repair product after discontinuation of use, that is, Ophiopogon japonicus root extract repairs the skin barrier and provides storage space for the NMF promoted by Dendrobium officinale stem extract. Dendrobium officinale stem extract promotes the formation of a breathable biofilm and the generation of NMF, providing support for the skin barrier improved by Ophiopogon japonicus root extract.

[0008] Preferably, the composition containing Camellia chrysantha extract includes the following components in parts by mass: 0.5-2 parts of Camellia chrysantha flower extract, 0.1-1 parts of Camellia chrysantha tea leaf extract, 0.1-0.5 parts of Camellia chrysantha essential oil, 0.1-1 parts of Ophiopogon japonicus root extract, and 0.1-1 parts of Dendrobium officinale stem extract.

[0009] The present invention has found that the dosage ratio of the components of the composition containing Camellia chrysantha extract has an impact on its barrier repair effect. When the dosage ratio of the components of the composition containing Camellia chrysantha extract is further selected to be within the above range, the barrier repair effect is even better.

[0010] Further preferably, the composition containing Camellia chrysantha extract includes the following components in parts by mass: 1 part of Camellia chrysantha flower extract, 0.5 part of Camellia chrysantha tea leaf extract, 0.3 part of Camellia chrysantha essential oil, 0.3 part of Ophiopogon japonicus root extract, and 0.5 part of Dendrobium officinale stem extract.

[0011] The present invention has found that when the components in the composition containing the Camellia chrysantha extract are used in the above dosage ratios, the barrier repair effect is optimal.

[0012] On the other hand, the present invention provides a method for preparing the above-mentioned composition containing Camellia chrysantha extract, comprising the following steps: mixing Camellia chrysantha flower extract, Camellia chrysantha tea leaf extract, Camellia chrysantha essential oil, Ophiopogon japonicus root extract and Dendrobium officinale stem extract to prepare the composition containing Camellia chrysantha extract.

[0013] In another aspect, the present invention provides use of the above-mentioned composition containing the Camellia chrysantha extract in the preparation of cosmetics.

[0014] Preferably, the cosmetic is any one of essence, cream or lotion.

[0015] Preferably, the amount of the composition containing the Camellia chrysantha extract added to the cosmetic is 0.1wt%-20wt%.The amount of the composition containing the Camellia chrysantha extract added is determined according to the type of cosmetic.

[0016] The present invention has found that the composition containing the Camellia chrysantha extract is added as an active ingredient to cosmetics to achieve multi-dimensional and long-lasting barrier repair functions of the cosmetics.

[0017] On the other hand, the present invention provides a skin barrier repair essence, comprising the following components in parts by mass: 1 to 10 parts of a composition containing Camellia chrysantha extract, 3 to 15 parts of glycerol, 1 to 10 parts of sorbitol, 1 to 15 parts of propylene glycol, 0.1 to 2 parts of parahydroxyacetophenone, 0.1 to 2 parts of hexylene glycol, 0.01 to 0.2 parts of EDTA-2Na, and 93.79 to 45.8 parts of water.

[0018] Preferably, the skin barrier repair essence comprises the following components in parts by mass: 3 parts of a composition containing Camellia chrysantha extract, 6 parts of glycerol, 3 parts of sorbitol, 4 parts of propylene glycol, 0.5 parts of parahydroxyacetophenone, 0.5 parts of hexylene glycol, 0.05 parts of EDTA-2Na, and 82.95 parts of water.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The components of the composition containing camellia chrysantha extract of the present invention work synergistically to achieve multi-dimensional and long-term synergistic repair of the skin barrier. First, linalool in camellia chrysantha essential oil penetrates efficiently, promotes keratinocyte proliferation and skin renewal, and at the same time opens transdermal channels for the other components; secondly, camellia chrysantha flower extract scavenges free radicals and instantly replenishes water, relieving tightness; camellia chrysantha tea extract inhibits inflammatory factors and blocks hyaluronidase expression, stabilizing the stratum corneum, that is, the combination of camellia chrysantha flower extract and camellia chrysantha tea extract reduces oxidative damage and inflammation, and stabilizes the stratum corneum; thirdly, ophiopogon root extract strengthens adhesion between keratinocytes, improves the skin barrier, and promotes the synthesis and reserve of skin natural moisturizing factor (NMF); dendrobium officinale stem extract promotes the formation of a breathable biofilm, significantly reduces water loss and upregulates filaggrin expression, continuously generating NMF, that is, ophiopogon root extract repairs the skin barrier and provides storage space for the NMF generated by the dendrobium officinale stem extract. The dendrobium officinale stem extract promotes the formation of a breathable biofilm and the generation of NMF, providing support for the skin barrier improved by the ophiopogon root extract. DETAILED DESCRIPTION

[0021] The present invention will be further described in detail below with reference to specific examples. The following examples are not intended to limit the present invention but are merely intended to illustrate the present invention. The experimental methods used in the following examples are generally based on conventional conditions unless otherwise specified. The materials and reagents used in the following examples are all commercially available unless otherwise specified.

[0022] The sources of raw materials involved in the following examples and comparative examples are as follows:

[0023] Camellia chrysantha extract: Ximuyuan (Xiamen) Biotechnology Co., Ltd.

[0024] Golden flower tea leaf extract: Xi'an Qiancao Biotechnology Co., Ltd.;

[0025] Ophiopogon root extract: Yantai Tongyuan Gonghe Biotechnology Co., Ltd.

[0026] Dendrobium officinale stem extract: Fufeng Snot Biotechnology Co., Ltd.

[0027] Camellia chrysantha essential oil: Commercially available Camellia chrysantha tea leaves and Camellia chrysantha flowers were dried in the shade and subjected to atmospheric steam distillation in a Clevenger apparatus for 5 hours. This was repeated five times. The distillates were combined and dissolved in n-hexane. Anhydrous sodium sulfate was added and allowed to stand for a while to absorb trace moisture. The n-hexane was removed using a nitrogen purge apparatus to obtain Camellia chrysantha essential oil.

[0028] Green tea extract: Shaanxi Harvard Biotechnology Co., Ltd.

[0029] Centella asiatica extract: Shanxi Lisheng Biotechnology Co., Ltd.

[0030] Lavender essential oil: Jiangxi Tianzhiyuan Spice Co., Ltd.

[0031] Oat extract: Guangzhou Shengrui Chemical Technology Co., Ltd.

[0032] Olive leaf extract: Hongte Chemical Co., Ltd.

[0033] Examples 1-5 are compositions containing Camellia chrysantha extract, and the components and their amounts by weight are shown in Table 1.

[0034] Table 1

[0035] Components Example 1 Example 2 Example 3 Example 4 Example 5 Camellia chrysantha Extract 1 0.5 2 0.01 3 Golden Flower Tea Extract 0.5 0.1 1 0.01 5 Camellia chrysantha essential oil 0.3 0.1 0.5 0.01 1 Ophiopogon root extract 0.3 0.1 1 0.05 2 Dendrobium officinale stem extract 0.5 0.1 1 0.01 2

[0036] The compositions containing Camellia chrysantha extract of Comparative Examples 1-15, the amounts of the components and their weight parts are shown in Table 2.

[0037]

[0038]

[0039] Application Examples and Comparative Application Examples

[0040] The application examples and comparative application examples of the present invention were used to verify the barrier repair effects of the compositions containing camellia chrysantha extract prepared in Examples 1-5 and Comparative Examples 1-15. The compositions containing camellia chrysantha extract of the above examples and comparative examples were prepared into essences. The components (by mass) were as follows: 3 parts of the composition containing camellia chrysantha extract, 6 parts of glycerin, 3 parts of sorbitol, 4 parts of propylene glycol, 0.5 parts of p-hydroxyacetophenone, 0.5 parts of hexylene glycol, and 0.05 parts of EDTA-2Na. Water was added to 100 parts by mass.

[0041] Preparation method of the essence: mix p-hydroxyacetophenone, hexylene glycol and propylene glycol, heat to 60°C to dissolve, cool to 45°C, add other ingredients, mix and stir evenly.

[0042] Effect experiment

[0043] 1. Test on the increase of filamentous protein by the composition containing golden camellia extract

[0044] HaCaT cells (Qingqi Biotechnology, BFN60803901) in good exponential growth phase were taken and 0.25% (v / v) trypsin digestion solution (Thermo Fisher Scientific, 15050-065) was added to digest the adherent cells to detach them. 1-4×10 5 / mL, make a cell suspension, inoculate in a 12-well plate, and culture in a CO2 incubator for 24 hours. The complete culture medium (Gibco) in the well was aspirated and 1×PBS was added. The experimental group and model group were exposed to UVB light for 15 minutes, while the control group was not irradiated; the 1×PBS solution was aspirated, and the experimental group was added with the composition of the embodiment or comparative example (concentration of 0.0125%), and the control group and model group were added with complete culture medium (Gibco) and cultured for 48 hours. The cells were collected, centrifuged to obtain the cell pellet, RIPA lysis buffer was added, and the cells were shaken 3 times with a vortex instrument (30s / time, interval of 3 minutes), and centrifuged at 12000rpm for 10 minutes. The supernatant was aspirated and tested using a FLG ELISA kit (Huamei Bio, CSB-EL008712HU). The test results are expressed as the FLG increase rate, and the calculation formula is: (experimental group-model group) / model group*100%.

[0045] 2. Skin barrier repair and moisturizing effect test

[0046] A number of volunteers aged 18-45 were selected. No skin care products were applied on the day of the experiment. After washing their faces, the subjects sat quietly in a constant temperature and humidity room at 21±1°C and 50±10% for 30 minutes. TM Hex (Courage+Khazaka) and moisture probe CM 825 (Courage + Khazaka) was used to test subjects' initial cleansing TEWL (TEWL) and moisture content. The subjects were randomly divided into 20 groups, each consisting of 5 participants. Each group was randomly assigned to use the serums from the present invention's application examples and comparative examples (the "Examples" and "Comparative Examples" in Table 3 refer to the corresponding application examples and comparative examples comprising the compositions of the embodiments and comparative examples) once daily in the morning. Each indicator was tested in the mornings of days 14 and 28. Improvements in TEWL and moisture content were analyzed, and the averaged data were used. The improvement was calculated as follows:

[0047] Improvement rate (%) relative to the initial value = |(after use - before use) / before use×100%|.

[0048] Table 3

[0049]

[0050] Comparative Examples 1-5 prove that each component of the composition containing Camellia chrysantha extract of the present invention is indispensable; Comparative Examples 6-10 prove that when the components containing Camellia chrysantha extract of the present invention are replaced with other ingredients with barrier repairing, hydrating and moisturizing properties, the barrier repairing effect is obviously inferior to the composition of the present invention; Comparative Examples 11-15 prove that the amount of each component in the composition of the present invention also has an important influence on the barrier repairing effect.

[0051] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. A composition containing camellia chrysantha extract, characterized in that it comprises the following components in parts by mass: 0.01-3 parts of camellia chrysantha flower extract, 0.01-5 parts of chrysantha tea leaf extract, 0.01-1 part of camellia chrysantha essential oil, 0.05-2 parts of ophiopogon root extract, and 0.01-2 parts of Dendrobium officinale stem extract.

2. The composition containing Camellia chrysantha extract according to claim 1 is characterized in that it comprises the following components in parts by mass: 0.5-2 parts of Camellia chrysantha flower extract, 0.1-1 parts of Camellia chrysantha tea leaf extract, 0.1-0.5 parts of Camellia chrysantha essential oil, 0.1-1 parts of Ophiopogon japonicus root extract, and 0.1-1 parts of Dendrobium officinale stem extract.

3. The composition containing Camellia chrysantha extract according to claim 2 is characterized in that it comprises the following components in parts by mass: 1 part of Camellia chrysantha flower extract, 0.5 part of Camellia chrysantha tea leaf extract, 0.3 part of Camellia chrysantha essential oil, 0.3 part of Ophiopogon japonicus root extract, and 0.5 part of Dendrobium officinale stem extract.

4. The method for preparing the composition containing the Camellia chrysantha extract according to any one of claims 1 to 3, characterized in that: The following steps are involved: The camellia chrysantha flower extract, camellia chrysantha tea leaf extract, camellia chrysantha essence, ophiopogon root extract and dendrobium officinale stem extract are mixed to prepare the camellia chrysantha extract-containing composition.

5. Use of the composition containing the Camellia chrysantha extract according to any one of claims 1 to 3 in the preparation of cosmetics.

6. The application according to claim 5, characterized in that The cosmetic is any one of essence, cream or emulsion.

7. The application according to claim 6, characterized in that The addition amount of the composition containing the Camellia chrysantha extract in the cosmetic is 0.1 wt%-20 wt%.

8. An essence, characterized in that it comprises the following components in parts by mass: 1-10 parts of a composition containing a Camellia chrysantha extract, 3-15 parts of glycerol, 1-10 parts of sorbitol, 1-15 parts of propylene glycol, 0.1-2 parts of p-hydroxyacetophenone, 0.1-2 parts of hexylene glycol, 0.01-0.2 parts of EDTA-2Na, and 93.79 parts to 45.8 parts of water.

9. The essence according to claim 8, characterized in that it comprises the following components, in parts by mass: 3 parts of a composition containing a Camellia chrysantha extract, 6 parts of glycerol, 3 parts of sorbitol, 4 parts of propylene glycol, 0.5 parts of p-hydroxyacetophenone, 0.5 parts of hexylene glycol, 0.05 parts of EDTA-2Na, and 82.95 parts of water.

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