Sensitive skin anti-aging mask matrix as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a sensitive skin anti-aging mask matrix as well as a preparation method and application thereof. According to the sensitive skin anti-aging mask product provided by the invention, grease and oil-soluble functional components are added on the basis of a traditional aqueous mask, so that the efficacy of the mask product is greatly improved. Meanwhile, the prepared mask is a nano-scale mask, compared with a traditional nano mask preparation process, the mask can be prepared by stirring at normal temperature simply, the average particle size of the prepared product is about 116 nm, and the permeability of the prepared product is better than that of a traditional aqueous mask. Through reasonable design and collocation, the oil-soluble component and the water-soluble component have a certain synergistic effect, so that the anti-aging composition has obvious effects in the aspects of moisturizing, relieving and anti-aging, is smaller in irritation and more friendly to people with sensitive skin, and meets the anti-aging requirements of people with sensitive skin from multiple angles, thereby having good practical application value.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a sensitive skin anti-aging facial mask matrix and a preparation method and application thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance understanding of the overall background of the invention and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to those skilled in the art.

[0003] Sensitive skin has a thinner stratum corneum, with a disordered arrangement and reduced lipid content between keratinocytes. This significantly impairs the skin's natural barrier function. This reduces the skin's resistance to external stimuli and makes it more susceptible to moisture loss. The immune system of sensitive skin is highly sensitive, and even the slightest external stimuli, such as temperature changes or irritating cosmetic ingredients, can trigger an inflammatory response. Inflammation not only exacerbates sensitive symptoms but also accelerates skin aging, leading to collagen degradation and damage to elastic fibers. With aging, cell turnover in sensitive skin slows further, resulting in insufficient new cell production and the accumulation of dead skin cells on the skin's surface, causing it to become rough, dull, and lack radiance. Therefore, the ideal face mask for sensitive skin should have simple, gentle ingredients, free of alcohol, fragrances, colorants, and harsh preservatives.

[0004] Products for sensitive skin often present the following two issues: 1. Risk of ingredient irritation: Many anti-aging ingredients, such as common retinol and high-concentration peptides, are highly active. While retinol is effective in boosting collagen production and improving fine lines, it can easily cause adverse reactions such as redness, peeling, and stinging, making it difficult for sensitive skin to tolerate. Some high-concentration peptide products can also irritate sensitive skin and trigger allergic reactions. In anti-aging masks, improper dosage of these ingredients, or lack of specialized sustained-release or encapsulation treatments, can place a significant burden on sensitive skin. 2. The difficulty in balancing moisturizing and anti-aging: Sensitive skin, due to a damaged barrier, already has insufficient moisturizing capacity, requiring a mask with strong hydrating properties. However, some anti-aging masks focus too much on anti-aging ingredients and neglect the inclusion of moisturizing ingredients. Insufficient moisturizing ingredients can result in skin feeling temporarily hydrated after use, but this moisture quickly drains away, leaving the skin dry and tight, compromising the anti-aging effect and potentially exacerbating sensitive symptoms. On the contrary, if the mask only emphasizes moisturizing, the anti-aging ingredients will be weak and cannot meet the anti-aging needs of sensitive skin. Summary of the Invention

[0005] In view of the deficiencies in the prior art, the present invention aims to provide a sensitive skin anti-aging facial mask matrix and its preparation method and application. It has been verified through experiments that the anti-aging facial mask for sensitive skin provided by the present invention has added oil and oil-soluble functional ingredients to the traditional aqueous facial mask, which greatly increases its efficacy. At the same time, the facial mask prepared by the present invention is a nano-scale facial mask. Compared with the traditional nano-mask, the preparation process is simple and can be prepared by stirring at room temperature. The average particle size of the prepared product is about 116nm, which has better permeability than the traditional aqueous facial mask. Through the reasonable design and matching of the present invention, there is a certain synergistic effect between the oil-soluble ingredients and the water-soluble ingredients, which has obvious effects in moisturizing, soothing and anti-aging, and is less irritating and more friendly to people with sensitive skin, thereby meeting the anti-aging needs of people with sensitive skin from multiple angles. Based on the above research results, the present invention is completed.

[0006] In order to achieve the above technical objectives, the technical solutions provided by the present invention are as follows: The first aspect of the present invention provides an anti-aging facial mask matrix for sensitive skin, which comprises the following components in mass fractions: 2-8% of polyol, 0.03-0.2% of chelating agent, 0.01-2% of rheology regulator, 0.1-1.5% of preservative, 1-5% of nano-inducer, 0.5-4% of liquid oil, 0.4-5% of oil-soluble active ingredient, 3-8% of water-soluble active ingredient, and the balance is water.

[0007] The second aspect of the present invention provides a method for preparing the above-mentioned skin-sense anti-aging mask matrix, which specifically comprises the following steps: S1. Preparation of the first solution: Add the above-mentioned polyol, chelating agent, thickener, preservative, and water-soluble active ingredient into water and stir and disperse until there are no particles; S2. Preparation of the second solution: stirring and mixing water, nano-inducing agent, liquid oil, and oil-soluble active ingredient; S3. Phase combination: add the second solution to the first solution while stirring, and stir to mix evenly.

[0008] Obviously, there is no particular order for steps S1 and S2.

[0009] The third aspect of the present invention provides the use of the above-mentioned sensitive skin anti-aging mask matrix in the preparation of cosmetics.

[0010] A fourth aspect of the present invention provides a cosmetic comprising the above-mentioned sensitive skin anti-aging facial mask matrix. The cosmetic can be in any known cosmetic dosage form, and is preferably a facial mask.

[0011] Beneficial technical effects of one or more of the above technical solutions: 1. Compared with other nanomaterials on the market (which require heating or high-pressure homogenization), the anti-aging facial mask matrix for sensitive skin prepared by the present invention has a simpler preparation method, can be prepared at room temperature and does not require high-pressure homogenization, which is more in line with the concept of green environmental protection.

[0012] 2. Through the reasonable design and matching of the present invention, there is a certain synergistic effect between the oil-soluble ingredients and the water-soluble ingredients, which has obvious effects in moisturizing, soothing and anti-aging. It is less irritating and more friendly to people with sensitive skin, and can meet the anti-aging needs of people with sensitive skin from multiple angles. Therefore, it has good practical application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The accompanying drawings, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0014] Figure 1 The reduction rate of a* value of different samples after 28 days is used in the present invention.

[0015] Figure 2 The relative expression of ROS fluorescence intensity in 3D epidermal model cells after application of different samples in the present invention.

[0016] Figure 3 The average particle size of the product prepared in Example 1 after three months of room temperature stability of the present invention. DETAILED DESCRIPTION

[0017] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention belongs.

[0018] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0019] In one typical embodiment of the present application, a sensitive skin anti-aging mask substrate is provided, which comprises the following components in mass fraction: polyol 2-8%, chelating agent 0.03-0.2%, rheology modifier 0.01-2%, preservative 0.1-1.5%, nano-inducing agent 1-5%, liquid oil 0.5-4%, oil-soluble active ingredient 0.4-5%, water-soluble active ingredient 3-8%, and the balance is water.

[0020] The polyol is any one or a combination of glycerol, butylene glycol, dipropylene glycol, and pentylene glycol; in an embodiment of the present application, the polyol is a combination of butylene glycol and dipropylene glycol, and the mass ratio of the two can be 0.5-5:1, preferably 1:1.

[0021] The chelating agent is any one or a combination of disodium EDTA and tetrasodium EDTA. The rheology modifier is any one or a combination of sodium polyacrylate, sodium polyacrylate grafted starch, xanthan gum, hydroxyethyl cellulose, polyacrylate crosspolymer-6, acryloyldimethyltaurine ammonium / VP copolymer, EMT 10, and SIMULEGEL EG; preferably a combination of xanthan gum and polyacrylate crosspolymer-6, and the mass ratio of the two can be 1-5:2-8, preferably 3:4.

[0022] The preservative is a combination of two or more of phenoxyethanol, frescolat ML, caprylyl oxyhydroxamic acid, ethylhexylglycerin, and 1,2-hexanediol; preferably a combination of caprylyl oxyhydroxamic acid and 1,2-hexanediol, and the mass ratio of the two is 1-5:1, preferably 2:1.

[0023] The nano-inducing agent is a combination of polyquaternium-61 and PPG-13-decyltetradecin polyether-24, and the mass ratio of the two is 0.01-0.2:0.5-3.

[0024] The liquid oil is one or more of isononyl isononanoate, C12-15 alcohol benzoate, trimethylolpropane triisostearate, caprylic / capric triglyceride, and dicaprylyl carbonate; specifically, a combination of isononyl isononanoate and caprylic / capric triglyceride, and the mass ratio of the two is 0.1-1:1, preferably 0.5:1.

[0025] The oil-soluble active ingredient is a combination of astaxanthin, shuyanyin (purchased from Beijing Galapagos Biotechnology Co., Ltd.), and camelina sativa seed oil. The preparation method of the camelina seed oil is as follows: the camelina seeds are removed from impurities, dried, and then cold-pressed to obtain virgin crude oil; the virgin crude oil is subjected to low-temperature hydration degumming, alkali refining and deacidification, and then decolorization and deodorization.

[0026] The drying is carried out at a low temperature of 35°C or below until the moisture content is less than 6%; The cold pressing process is carried out at room temperature, preferably controlling the oil press speed to 20-50 rpm (preferably 40 rpm) and the pressure not exceeding 25 MPa; The specific conditions of the low-temperature hydration degumming are: adding water at 40-60°C (preferably 50°C), and the amount of water added is controlled to be 1-5% (preferably 2.8%) of the crude oil mass; The specific conditions of the alkali refining and deacidification are: controlling the sodium hydroxide concentration to 0.05-0.2 mol / L and the temperature to 40-60° C. (preferably 50° C.).

[0027] The decolorization process specifically comprises performing low-temperature decolorization treatment using activated clay at 40-50°C (preferably 45°C), with the addition amount of the activated clay being 0.1-2% (preferably 8%); The specific conditions of the deodorization process are: deodorization treatment is carried out under the conditions of 0.2-0.8 kPa vacuum degree (preferably 0.5 kPa vacuum degree) and 100-120° C. (preferably 110° C.).

[0028] The water-soluble active ingredient is a combination of panthenol, keratinase, brown algae probiotic fermentation liquid and lactobacillus fermentation product; further, the mass ratio of panthenol, keratinase, brown algae probiotic fermentation liquid and lactobacillus fermentation product is 0.5-2:0.5-2:1-3:0.1-1. In the present invention, the keratinase is 0.5-5% (preferably 1%). w / w ) Aqueous solution of keratinase.

[0029] The brown algae probiotic fermentation broth can be obtained commercially or prepared by itself, and can be specifically prepared by referring to the preparation method in CN108653059A.

[0030] The lactobacillus fermentation product is specifically a lactobacillus vesicle powder, which is obtained by extracting and freeze-drying vesicles of Lactobacillus plantarum. The specific method for extracting and obtaining the vesicles of Lactobacillus plantarum can be prepared by sucrose density gradient centrifugation. The Lactobacillus plantarum is specifically Lactobacillus plantarum CICC 25283.

[0031] In another embodiment of the present invention, a method for preparing a matrix of an anti-aging facial mask for sensitive skin is provided, which specifically comprises the following steps: S1. Preparation of the first solution: Add the above-mentioned polyol, chelating agent, thickener, preservative, and water-soluble active ingredient into water and stir and disperse until there are no particles; S2. Preparation of the second solution: stirring and mixing water, nano-inducing agent, liquid oil, and oil-soluble active ingredient; S3. Phase combination: add the second solution to the first solution while stirring, and stir to mix evenly.

[0032] Obviously, there is no particular order for steps S1 and S2.

[0033] The above preparation processes are all carried out at room temperature.

[0034] In step S2, the mass fraction of water in the sensitive skin anti-aging mask matrix is ​​1-4%; In step S3, the stirring speed is 80-120 rpm, and the stirring time is 3-8 min.

[0035] In another specific embodiment of the present invention, there is provided use of the above-mentioned sensitive skin anti-aging mask matrix in the preparation of cosmetics.

[0036] In another specific embodiment of the present invention, a cosmetic is provided, comprising the above-mentioned sensitive skin anti-aging mask matrix. The cosmetic can be in any known cosmetic dosage form, particularly a mask dosage form.

[0037] The cosmetics may also contain any other raw material ingredients known in the cosmetics field, which are not specifically limited here.

[0038] The present invention is further described below by examples and drawings, but the present invention is not limited to the scope of the embodiments described. Based on the embodiments of the present invention, any changes to the present invention made by those skilled in the art without making any creative ideas shall fall within the scope of protection of the present invention. At the same time, in the embodiments of the present invention, unless otherwise specified, all preparation raw materials are commercially available products familiar to those skilled in the art. In the embodiments of the present invention, the keratinase used is Phyto-Keratinase (ST) (its specific composition is: 97% water, 2% 1,2-hexanediol, 1% keratinase, w / w ), which was purchased from Givaudan Fragrances & Fragrances (Shanghai) Co., Ltd. The INCI name of Erythcalm SC used is: Caprylic / Capric Triglyceride, Saururus Chinesis Extract, which was purchased from Shanghai Jiakai Biological Co., Ltd.

[0039] The preparation processes of the linseed oil in Examples 1-5 and Comparative Example 2 are as follows: Camelina seeds are first air-sorted and screened to remove impurities, then oven-dried below 35°C to a moisture content of less than 6%. Crude oil is then obtained by cold pressing at room temperature, with the press speed controlled at 40 rpm and the pressure not exceeding 25 MPa. The resulting crude oil undergoes low-temperature hydration degumming (50°C, 2.8% water added by weight), alkali refining and deacidification (0.15 mol / L sodium hydroxide, 50°C), followed by low-temperature bleaching at 45°C using activated clay (0.8%), and finally deodorization at 110°C under a vacuum of 0.5 kPa to produce camelina seed oil. The brown algae probiotic fermentation broth in the Examples and Comparative Examples is the seaweed fermentation broth obtained by the preparation method of Example 1 of CN 108653059A.

[0040] The preparation process of the lactobacillus vesicle powder in the examples and comparative examples is as follows: Lactobacillus plantarum (CICC 25283) was inoculated into MRS liquid medium (pH 6.2-6.4) and cultured anaerobically in a 37°C constant temperature incubator until the middle of the logarithmic growth phase (OD 600 The culture medium should reach a value of 0.6-0.8 after approximately 12-16 hours of culture. Transfer the culture medium to a centrifuge tube and centrifuge at 8,000 × g for 10 minutes at 4°C. Discard the supernatant and resuspend the cells in pre-chilled phosphate-buffered saline (PBS, pH 7.4, containing 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, and 2 mM KH₂PO₄). Repeat the centrifugation wash twice, and finally resuspend the cells in PBS at 1 / 10 the original volume of the culture medium. Place the bacterial suspension in an ice-water bath and use an ultrasonic disruptor (power 300 W, pulse mode: disrupt for 3 seconds, pause for 5 seconds) for a total of 15 minutes, until the solution becomes significantly clear (or the bacterial disruption rate is >90% as observed by microscopy). Centrifuge the disrupted suspension at 15,000 × g for 30 minutes at 4°C. Discard the pellet (cell debris and unbroken cells) and collect the supernatant. Transfer the supernatant to an ultracentrifuge tube and ultracentrifuge at 120,000 × g for 90 minutes at 4°C. Discard the supernatant and resuspend the pellet in 1-2 mL of pre-chilled PBS (gently pipetting to avoid rupturing the vesicles). Filter the resuspension through a 0.22 μm filter to sterilize the solution and further purify it by sucrose density gradient centrifugation (prepare a 10%-60% sucrose gradient solution, layer the vesicle suspension on top, centrifuge at 100,000 × g for 16 hours at 4°C, and collect the vesicle layer with a density of approximately 1.10-1.15 g / mL). Finally, dialyze against PBS to remove the sucrose to obtain purified Lactobacillus vesicles, which are then freeze-dried to obtain Lactobacillus vesicle powder.

[0041] The raw material compositions of Examples 1 to 5 and Comparative Examples 1 to 5 are shown in Table 1.

[0042] Table 1 List of raw material compositions of Examples and Comparative Examples

[0043] Note: The camelina seed oil preparation process of Comparative Example 4 is as follows: camelina seeds are first air-selected and screened to remove impurities, then dried at a high temperature below 80°C to a moisture content of less than 6%. Crude oil is then obtained by cold pressing at room temperature, with the press speed controlled at 70 rpm and the pressure not exceeding 60 MPa. The resulting crude oil is then subjected to low-temperature hydration degumming (45°C, 3.5% water added by weight), alkali refining and deacidification (0.25 mol / L sodium hydroxide, 45°C), and low-temperature bleaching at 45°C using activated clay (0.8%). Finally, deodorization is performed at 110°C under a vacuum of 0.5 kPa to produce camelina seed oil. The camelina seed oil preparation process of Comparative Example 5 is as follows: camelina seeds are first air-selected and screened to remove impurities, then low-temperature dried at below 35°C to a moisture content of less than 6%. Crude oil is then cold-pressed at room temperature, with the press speed controlled at 20 rpm and the pressure not exceeding 25 MPa, to obtain virgin crude oil. The resulting crude oil is then subjected to low-temperature hydration degumming (35°C, 5.8% water added by weight), alkali refining and deacidification (0.6 mol / L sodium hydroxide, 70°C), and low-temperature bleaching using activated clay (0.8%) at 45°C. Finally, deodorization is performed at 110°C under a vacuum of 0.5 kPa to produce camelina seed oil. The preparation method of the facial mask matrix samples described in Examples 1 to 5 and Comparative Examples 1 to 5 comprises the following steps: S1. Preparation of solution 1: Add polyol, chelating agent, thickener, preservative, and water-soluble active ingredient into water at room temperature, stir and disperse until no particles are formed, and set aside; S2, solution 2 preparation: under room temperature conditions, water, nano-inducing agent, liquid oil, and oil-soluble active ingredient are stirred and mixed uniformly, and set aside; S3. Mixing phase: Under normal temperature conditions, add solution 2 to solution 1 while stirring, and stir to mix evenly at a stirring speed of 100 rpm for 6 minutes to obtain a sensitive skin anti-aging mask matrix.

[0044] Test Example 1: Irritation Evaluation Test The experiment recruited 30 subjects, both male and female, aged 23 to 50 years old, and used Examples 1 to 5 as test substances, and blank as negative control. The test method was to select an area not exceeding 50 mm 2Using a closed patch test method, approximately 0.020-0.025g of the test substance was applied to a suitable patch tester with a depth of approximately 1mm. Hypoallergenic tape was applied to the subject's back. Each subject tested a blank control group, samples from Examples 1-5, and 6. After 24 hours, the test substance was removed. Skin reactions were observed 0.5, 24, and 48 hours after removal. Results were recorded according to the skin reaction grading standards in the "2015 Cosmetic Safety Technical Specifications." Detailed results are shown in Table 2 below.

[0045]

[0046] Table 2 Results of irritation evaluation test

[0047] As can be seen from Table 2, the samples (Examples 1 to 5) provided by the present invention are relatively mild and non-irritating to the skin.

[0048] Test Example 2: Human Efficacy Test Test Information

[0049] (1) Moisturizing performance test Testing Method: This experiment measures the moisturizing effect of the test sample by measuring the water content in the skin's stratum corneum. Skin moisture content was tested using the German CK Corneometer CM 825. Subjects with similar skin moisture content were screened for cleansing and the moisture content of the forehead, left cheek, and right cheek was tested, with the arithmetic mean calculated. The mask was then applied to the face, removed after 15 minutes, and the excess mask fluid was gently patted for absorption. The skin moisture content of the same locations on the forehead, left cheek, and right cheek was then tested using a skin moisture tester, with the arithmetic mean calculated. The skin moisture content was tested at 0h, 8h, and 24h after mask removal, with the arithmetic mean calculated for each group of subjects.

[0050] Skin moisture content increase rate (%) = [(A-A0] / A0×100%, A0 is the arithmetic mean of skin moisture content before applying the mask, and A is the arithmetic mean of skin moisture content measured at a specific time.

[0051] Table 3 Skin moisture content increase rate in different time periods in Examples and Comparative Examples

[0052] As can be seen from Table 3, the ability of adding only one type of active ingredient (oil-soluble active ingredient or water-soluble active ingredient) to increase skin moisture content is far lower than adding both types of active ingredients simultaneously. This indicates that the combination of oil-soluble and water-soluble active ingredients has a certain synergistic effect. Furthermore, Example 1 was able to increase skin moisture content after 4 and 8 hours, demonstrating that the facial mask of the present invention not only has a good moisturizing effect but also provides long-lasting moisturizing. The different camelina seed oil preparation processes (comparing Example 1 with Comparative Examples 4 and 5) also showed certain differences in the rate of increase in skin moisture content. This indicates that the camelina seed oil preparation process provided in Example 1 is superior, capable of better utilizing the efficacy of camelina seed oil and providing better moisturizing effects.

[0053] (2) Soothing evaluation experiment How to use: Replace your daily facial mask and use it every other day. Keep other skin care habits unchanged for 28 consecutive days.

[0054] Evaluation Method: The VISIA-CR Facial Image Analyzer (Canfield, USA) provides safe and stable standard lighting conditions. All camera settings and imaging parameters are controlled by software to ensure stable and standardized imaging. The instrument features multiple light source modes: standard light, blue light, orange light, cross-polarized light, and parallel polarized light, enabling multi-angle imaging of facial skin with image quality up to 21 megapixels. This test provides images of the subject's face captured under three light sources: standard light 1, standard light 2, and cross-polarized light, as well as red zone images derived under cross-polarized light. The red zone images were analyzed to derive a quantitative indicator, the red zone analysis a* value, which was used to assess improvements in facial redness and sensitivity. The specific results are shown below.

[0055] Depend on Figure 1 It can be seen that after 28 days of use, a mask base without any active ingredients not only failed to provide a soothing effect but actually increased skin sensitivity. However, the addition of active ingredients significantly reduced the a* value, achieving a certain soothing effect. In particular, the ability to reduce the a* value by adding only one type of active ingredient (either oil-soluble or water-soluble) was far less than that achieved by adding both active ingredients simultaneously. This demonstrates that the combination of oil-soluble and water-soluble active ingredients has a synergistic effect, and the mask base provided by the present invention can achieve excellent and long-lasting soothing effects. Different camelina seed oil preparation processes (comparing Example 1 with Comparative Examples 4 and 5) also showed some differences in the rate of increase in skin moisture content, demonstrating that the camelina seed oil preparation process provided in Example 1 is superior, effectively leveraging the efficacy of camelina seed oil and providing a good soothing effect.

[0056] Test Example 3: Antioxidant Capacity Test (1) Experimental materials and instruments The 3D epidermal model was derived from a commercially available, functionally stable human reconstructed 3D epidermal model. After transportation, the 3D epidermal model was placed in a 12-well plate for stable culture, with 2 ml of maintenance culture medium per well. The culture conditions were 37°C, 5% CO2, and saturated humidity. The model was used after stable culture for 12 hours.

[0057] (2) Main reagents Epidermal model maintenance medium and epidermal model detection medium were purchased from Shanghai Si'anfuno Biotechnology Co., Ltd., the ROS fluorescence detection kit was purchased from Beyotime Biotechnology, and PBS was purchased from Sinopharm Chemical Reagent Co., Ltd.

[0058] (3) Main instruments upright microscope (ZESS, Germany).

[0059] (4) Samples to be tested Example 1, Comparative Examples 1 to 3 (5) Test method For the treated 3D epidermal model, cells were washed three times with PBS for 5 minutes each wash. Using a ROS fluorescent staining kit, DCFH-DA was diluted 1:1000 in serum-free culture medium to a final concentration of 10 μmol / L. The cells were incubated in a 37°C cell culture incubator for 20 minutes. Mixing was performed every 3-5 minutes to ensure adequate contact between the probe and the cells. The cells were then washed three times with serum-free culture medium to fully remove any DCFH-DA that had not entered the cells. Green fluorescence was observed using a fluorescence microscope at 488 nm, and fluorescence intensity was calculated using ImageJ.

[0060] (6) Test results The cells in the well plate were stained with ROS fluorescence in vivo, photographed with a fluorescence microscope, and the fluorescence intensity was counted. Figure 2 shown.

[0061] The ability to reduce ROS by adding only one type of active ingredient (either oil-soluble or water-soluble) was significantly lower than when both types were added simultaneously. This suggests that the combination of oil-soluble and water-soluble active ingredients has a synergistic effect, and that the facial mask base provided by the present invention can achieve excellent antioxidant efficacy. Different camelina seed oil preparation processes (comparing Example 1 with Comparative Examples 4 and 5) also showed some differences in the rate of increase in skin moisture content. This suggests that the camelina seed oil preparation process provided in Example 1 is superior, effectively leveraging the efficacy of camelina seed oil and providing excellent antioxidant benefits.

[0062] Test Example 4: Sample stability test The samples of Examples 1 to 5 were placed at room temperature, -18°C, 4°C, 45°C, and subjected to a thermal cycle (-18°C, 4°C, 45°C, cycled every 3 days) for the corresponding periods of time for stability observation. The stability of the product was determined by whether oil production, turbidity, or other instability occurred. If no instability such as oil production or turbidity occurred, the product passed the stability test. If not, the reason for failure was indicated. The specific results are shown in Table 4 below.

[0063] Table 4 Stability test results of different samples

[0064] As shown in the table above, the facial mask bases provided by the present invention (Examples 1-5) exhibit excellent stability. Due to differences in the composition of the oils obtained using different camelina seed oil preparation processes (comparing Examples 4 and 5), the stability of Examples 4 and 5 was slightly lower in the stability test and failed the test. This demonstrates that the camelina seed oil preparation processes provided by the examples of the present invention are relatively superior and suitable for the system prepared by the present invention.

[0065] Test Example 4: Sample Particle Size Test The particle size of the sample after the stability test of Example 1 (hot and cold cycle, room temperature, -18 ° C, 4 ° C, 45 ° C, the above sample was diluted 10 times and tested) was measured using a Zeta sizer nanoZS nanoparticle size analyzer. The specific results are as follows: Figure 3 and as shown in Table 5.

[0066] Table 5 Average particle size of the sample after stabilization in Example 1

[0067] As shown in Table 5, under room temperature conditions, the average particle size of Example 1 provided by the present invention is about 116 nm, which shows a certain penetration ability. Moreover, after 3 months of stability testing, the particle size of the sample of Example 1 did not change much, indicating good product stability.

[0068] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A sensitive skin anti-aging mask matrix, characterized in that: The sensitive skin anti-aging mask matrix contains the following components in mass fractions: 2-8% polyol, 0.03-0.2% chelating agent, 0.01-2% rheology regulator, 0.1-1.5% preservative, 1-5% nano inducer, 0.5-4% liquid oil, 0.4-5% oil-soluble active ingredient, 3-8% water-soluble active ingredient, and the balance is water.

2. The anti-aging facial mask matrix for sensitive skin according to claim 1, characterized in that: The polyol is any one of glycerol, butylene glycol, dipropylene glycol, and pentylene glycol, or a combination of more than one thereof; The chelating agent is any one of disodium EDTA and tetrasodium EDTA, or a combination of more than one thereof; The rheology modifier is any one of sodium polyacrylate, sodium polyacrylate grafted starch, xanthan gum, hydroxyethyl cellulose, polyacrylate crosspolymer-6, ammonium acryloyldimethyltaurate / VP copolymer, EMT 10, and SIMULEGEL EG, or a combination thereof; The preservative is two or more of phenoxyethanol, phenoxyethanol, capryloylhydroxamic acid, ethylhexylglycerin, and 1,2-hexanediol; The nano inducer is a combination of polyquaternium-61 and PPG-13-decyltetradecyl alcohol polyether-24; The oil-soluble active ingredient is a combination of astaxanthin, xiuyanin, and camelina seed oil; The water-soluble active ingredient is a combination of panthenol, keratinase, brown algae probiotic fermentation liquid and lactobacillus fermentation product.

3. The anti-aging facial mask matrix for sensitive skin according to claim 2, characterized in that: The preparation method of the camelina seed oil is as follows: the camelina seeds are removed from impurities, dried, and then cold-pressed to obtain virgin crude oil; the virgin crude oil is subjected to low-temperature hydration degumming, alkali refining and deacidification, and then decolorization and deodorization.

4. The sensitive skin anti-aging mask matrix according to claim 3, characterized in that: The drying is carried out at a low temperature of 35°C or below until the moisture content is less than 6%; The cold pressing process is carried out at room temperature, preferably controlling the oil press speed to 20-50 rpm and the pressure not exceeding 25 MPa; The specific conditions of the low-temperature hydration degumming are: adding water at 40-60°C, and controlling the amount of water added to be 1-5% of the crude oil mass; The specific conditions of the alkali refining and deacidification are: controlling the sodium hydroxide concentration to 0.05-0.2 mol / L and the temperature to 40-60°C; The decolorization process specifically comprises performing low-temperature decolorization treatment at 40-50°C using activated clay, with the addition amount of the activated clay being 0.1-2%; The specific conditions of the deodorization process are: deodorization treatment is carried out under the conditions of 0.2-0.8 kPa vacuum degree and 100-120°C.

5. The anti-aging facial mask matrix for sensitive skin according to claim 2, characterized in that: The lactobacillus fermentation product is specifically lactobacillus vesicle powder, which is obtained by extracting and freeze-drying the vesicles of Lactobacillus plantarum.

6. The anti-aging facial mask matrix for sensitive skin according to claim 5, characterized in that: The specific method for extracting and obtaining Lactobacillus plantarum vesicles is to prepare them by sucrose density gradient centrifugation, and the Lactobacillus plantarum is Lactobacillus plantarum CICC25283.

7. The method for preparing the sensitive skin anti-aging mask matrix according to any one of claims 1 to 6, characterized in that: The specific steps include: S1. Preparation of the first solution: adding part of the above-mentioned polyol, chelating agent, thickener, preservative, and water-soluble active ingredient into water and stirring and dispersing until there are no particles; S2. Preparation of the second solution: stirring and mixing the remaining polyol, water, nano-inducer, liquid oil, and oil-soluble active ingredient; S3, phase combination: adding the second solution to the first solution under stirring conditions, stirring and mixing until uniform; There is no particular order in which steps S1 and S2 are performed.

8. The preparation method according to claim 7, wherein The preparation process is carried out at room temperature; In step S3, the stirring speed is 80-120 rpm, and the stirring time is 3-8 min.

9. Use of the sensitive skin anti-aging mask matrix according to any one of claims 1 to 6 in the preparation of cosmetics.

10. A cosmetic, characterized in that: The cosmetic comprises the sensitive skin anti-aging mask matrix according to any one of claims 1 to 6; further, the cosmetic is a facial mask.

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