STR integrated DNA chip
By integrating DNA extraction, PCR reaction and electrophoresis functions, the STR integrated DNA chip solves the problem of DNA electrophoresis detection being sensitive to environmental conditions, realizes convenient DNA detection and shortens detection time.
Patent Information
- Application Number
- CN202511036252.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-26
- Publication Date
- 2025-10-10
AI Technical Summary
The capillaries required for DNA electrophoresis testing are sensitive to environmental conditions such as movement, vibration, and temperature. The test is only applicable to laboratory environments and takes a long time.
It adopts STR integrated DNA chip, which integrates double-layer substrate, DNA extraction and purification module, driving air pressure control chip, PCR amplification module, fluorescence detection window and microfluidic plate pipeline, and uses new microfluidic technology to realize the integration of DNA extraction, PCR reaction and electrophoresis functions, replacing traditional capillary operation.
It realizes DNA testing that is easy to operate and preserve in different environments, shortens the detection time, and reduces dependence on the laboratory environment.
Smart Images
Figure CN120758339A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of DNA detection, and in particular to a STR integrated DNA chip. Background Art
[0002] PCR is a technique for amplifying DNA and can be used to identify individuals based on the amplification of small regions of the human genome containing a class of repetitive DNA called short tandem repeats (STRs). There are hundreds of thousands of STR loci in the human genome, many of which are highly polymorphic. STR analysis has become a staple of forensic science, with applications in a growing range of areas, including law enforcement, paternity testing, identification of individuals in large-scale disasters, and routine typing of children.
[0003] In the early years, due to limitations in process materials, biochips had the following major drawbacks and limitations: their small size and simple structure, which made them incapable of simulating all the processes required for STR experiments. Subsequently, a new generation of biochips, represented by DNAscan in the United States, integrated a continuous DNA preparation and purification structure, amplification, and electrophoresis, along with the corresponding reagents processed using freeze-drying technology. This process also shortened the PCR thermal cycle to 18 minutes, and finally transferred the amplified product to a fluorescence detection window. This process requires no sample processing outside the DNA chip, no manual steps, no additional reagents, and no manual analysis of the DNA products produced during the process.
[0004] Previously, it was a DNA electrophoresis test, but the capillaries required for electrophoresis are sensitive to environmental conditions such as movement, vibration, and temperature, so the test is only suitable for laboratory environments. Summary of the Invention
[0005] The purpose of the present invention is to provide an STR integrated DNA chip to solve the problem that the capillary required for DNA electrophoresis detection is sensitive to environmental conditions such as movement, vibration, and temperature, and the detection is only applicable to laboratory environments and takes a long time.
[0006] In order to achieve the above object, the present invention provides the following technical solutions: A STR integrated DNA chip comprises a double-layer substrate, a DNA extraction and purification module, a driving air pressure control chip, a PCR amplification module, a fluorescence detection window, and a microfluidic plate pipeline. The DNA extraction and purification module is provided on the left side of the upper portion of the double-layer substrate, the driving air pressure control chip is provided on the right side of the DNA extraction and purification module, the PCR amplification module is provided on the rear side of the driving air pressure control chip, the fluorescence detection window is provided on the reverse side of the DNA extraction and purification module, and the microfluidic plate pipeline is provided inside the double-layer substrate.
[0007] Furthermore, a swab insertion cavity is provided on the upper portion of the DNA extraction and purification module, and an electrophoresis gel box is provided on the rear portion of the DNA extraction and purification module.
[0008] Furthermore, a plurality of microfluidic plate channels with a channel diameter of 80 microns are provided at the fluorescence detection window.
[0009] Furthermore, the microfluidic plate channel is formed by sealing the channel with two layers of COP material films using nanoimprint technology.
[0010] The beneficial effects of the present invention are as follows: the present application uses novel microfluidic technology to replace the function of the capillary in DNA electrophoresis, and pre-installs the DNA extraction function, PCR reaction function, and electrophoresis function in an integrated biochip, which is easy to operate and can work and be stored in different environments.
[0011] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the present invention with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] Figure 1 It is a schematic diagram of the overall front structure of an embodiment of the present invention.
[0013] Figure 2 It is a schematic structural diagram of the overall reverse side of an embodiment of the present invention.
[0014] Figure 3 for Figure 1 Schematic diagram of the structure of component 2.
[0015] Figure 4 for Figure 2 Schematic diagram of the structure of component 5.
[0016] Figure 5 for Figure 2 Schematic diagram of the structure of the middle component 6.
[0017] Explanation of the accompanying symbols: 1. Double-layer substrate; 2. DNA extraction and purification module; 3. Driving air pressure control chip; 4. PCR amplification module; 5. Fluorescence detection window; 6. Microfluidic plate pipeline; 7. Electrophoresis gel box. DETAILED DESCRIPTION
[0018] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.
[0019] In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second", "third" are only for descriptive purposes and cannot be understood as indicating or implying relative importance.
[0020] In the description of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be connected inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.
[0021] In addition, the technical features involved in the different embodiments of the application described below can be combined with each other as long as they do not conflict with each other.
[0022] Please refer to Figure 1 , a STR integrated DNA chip shown in a preferred embodiment of the present application, comprising a double-layer substrate 1, a DNA extraction and purification module 2, a driving air pressure control chip 3, a PCR amplification module 4, a fluorescence detection window 5, a microfluidic plate pipeline 6, the upper left side of the double-layer substrate 1 is provided with the DNA extraction and purification module 2, the right side of the DNA extraction and purification module 2 is provided with the driving air pressure control chip 3, the rear side of the driving air pressure control chip 3 is provided with the PCR amplification module 4, the opposite side of the DNA extraction and purification module 2 is provided with the fluorescence detection window 5, and the inside of the double-layer substrate 1 is provided with the microfluidic plate pipeline 6.
[0023] The upper part of the DNA extraction and purification module 2 is provided with a swab insertion cavity, and the rear part of the DNA extraction and purification module 2 is provided with an electrophoresis gel box 7.
[0024] The fluorescence detection window 5 is provided with a plurality of microfluidic plate pipelines 6 with a diameter of 80 microns.
[0025] The microfluidic plate pipeline 6 is a two-layer COP material film sealed by nano-imprinting technology.
[0026] In use, place the STR integrated DNA chip, put the extracted DNA test material into the swab insertion cavity, start the DNA rapid detection instrument, and wait for the instrument detection result.
[0027] In summary, the present invention provides an integrated STR DNA chip. This device uses novel microfluidic technology to replace the function of capillaries in DNA electrophoresis, pre-installing DNA extraction, PCR reaction, and electrophoresis functions in an integrated biochip. This makes it easy to operate and can be used and stored in different environments.
[0028] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0029] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.
Claims
1. A STR integrated DNA chip, characterized in that: The invention comprises a double-layer substrate (1), a DNA extraction and purification module (2), a driving air pressure control chip (3), a PCR amplification module (4), a fluorescence detection window (5), and a microfluidic plate pipeline (6). The DNA extraction and purification module (2) is provided on the left side of the upper part of the double-layer substrate (1), the driving air pressure control chip (3) is provided on the right side of the DNA extraction and purification module (2), the PCR amplification module (4) is provided on the rear side of the driving air pressure control chip (3), the fluorescence detection window (5) is provided on the reverse side of the DNA extraction and purification module (2), and the microfluidic plate pipeline (6) is provided inside the double-layer substrate (1).
2. The STR integrated DNA chip according to claim 1, characterized in that: A swab insertion cavity is provided on the upper portion of the DNA extraction and purification module (2), and an electrophoresis gel box (7) is provided on the rear portion of the DNA extraction and purification module (2).
3. The STR integrated DNA chip according to claim 1, characterized in that: The fluorescence detection window (5) is provided with a plurality of microfluidic plate channels (6) each having a channel diameter of 80 microns.
4. The STR integrated DNA chip according to claim 1, characterized in that: The microfluidic plate channel (6) is formed by sealing the channel with two layers of COP material films using nanoimprinting technology.