Industrial-grade continuous preparation process capable of efficiently producing high-purity highland barley beta-glucan

Abstract

The invention relates to the field of glucan, and particularly discloses an industrial-grade continuous preparation process capable of efficiently producing high-purity highland barley beta-glucan, and the industrial-grade continuous preparation process comprises the following steps: S1, crushing; s2, performing extraction and enzymolysis: performing extraction and enzymolysis by using 0.4-1.0 wt% of alpha-amylase for 2-3 times at the temperature of 65-85 DEG C for 2-6 hours according to the solid-to-liquid ratio of 1: (12-20); s3, alcohol precipitation: carrying out alcohol precipitation on the supernate obtained in the step S2 by adopting an ethanol solution with the concentration of 40-60wt%; s4, purification: the temperature is 30-50 DEG C, the purification time is 2-12 h, the solid-to-liquid ratio is 1: (60-90), 0.15-0.25 wt% of alpha-amylase is used for purification, and the highland barley beta-glucan is obtained. The purity of the highland barley beta-glucan obtained through the preparation process can reach 80.35%, and the significant progress of reducing cost and improving efficiency is achieved.

Description

Technical Field

[0001] The present application relates to the technical field of glucan, and in particular to an industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan. Background Art

[0002] Highland barley, a variety of the wheat family of the Poaceae family, is one of the world's oldest crops, boasting a long history of cultivation. Cultivation and utilization began thousands of years ago. Over time, it has become a staple food, with a history spanning over 3,500 years. Highland barley is rich in a variety of nutrients, including protein, β-glucan, fiber, and vitamins. It also contains 18 amino acids, multiple unsaturated fatty acids, and certain minerals. These nutrients not only contribute to its high nutritional value but also offer broad potential for the development of derivative products. Since the commencement of highland barley hybrid breeding in 1965, the tireless efforts of breeders have resulted in the development of a series of key varieties, including "Tibetan Blue 320" and "Tibetan Blue 2000." Furthermore, highland barley-based products, such as Cordyceps wine and Rhodiola rosea wine, have been developed. These products, which promote immune system regulation and fatigue relief, have further boosted the highland barley industry. As an important extract of highland barley, highland barley β-glucan plays a key role in fully realizing the nutritional value of highland barley.

[0003] In the existing highland barley β-glucan preparation technology, for the extraction process, the raw materials are usually first added to water to allow it to react, and then specific enzymes are added to perform enzymatic hydrolysis. This step-by-step process is relatively complicated. In addition, there is also a practice of using other common enzymes to extract or hydrolyze the crushed highland barley separately under certain conditions, and then the products of both are subjected to subsequent treatment. In the purification process, ethanol is basically used for purification, and the amount of ethanol used is relatively large. In some processes, after the extraction and enzymatic hydrolysis are completed, the mixed solution will undergo multiple separation and concentration processes, and then a large amount of ethanol will be added to precipitate the crude β-glucan extract, which is then subsequently refined. In other processes, the crude extract is subjected to multi-stage ethanol purification in the hope of improving the purity of the β-glucan.

[0004] The existing highland barley β-glucan preparation technology has the problem of high cost, which makes mass production difficult. Even if mass production can be achieved, the purity of the final highland barley β-glucan can only reach around 60%, which cannot meet actual requirements. Summary of the Invention

[0005] In order to solve the above technical problems, the present application provides an industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan.

[0006] The present application provides an industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan, comprising the following steps: S1, crushing: crushing highland barley rice; S2, extraction and enzymolysis: under the conditions of a temperature of 65-85°C, an extraction time of 2-6h, and a solid-liquid ratio of 1:(12-20), the highland barley rice after the crushing in the step S1 is extracted and enzymolyzed using an α-amylase solution to obtain a mixed solution, wherein the amount of α-amylase in the α-amylase solution is 0.4-1.0wt% of the total amount of the crushed highland barley rice, and the number of extraction and enzymolysis is 2-3 times; S3, alcohol precipitation: the mixed solution obtained in step S2 is allowed to stand to obtain a supernatant, which is filtered and concentrated, and alcohol precipitation is performed using a 40-60 wt% ethanol solution to obtain a suspension of crude β-glucan extract, which is then filtered to obtain a solid substance, which is dried and crushed to obtain a crude β-glucan extract; S4, purification: Under the conditions of a temperature of 30-50°C, a purification time of 2-12 hours, and a solid-liquid ratio of 1:(60-90), the crude β-glucan extract in step S3 is purified using an α-amylase solution, filtered to obtain a solid substance, dried, and crushed to obtain highland barley β-glucan, wherein the amount of α-amylase in the α-amylase solution is 0.15-0.25wt% of the total amount of the crude β-glucan extract.

[0007] Preferably, in step S2, the temperature is 75°C.

[0008] Preferably, in step S2, the extraction time is 2 hours.

[0009] Preferably, in step S2, the solid-liquid ratio is 1:14.

[0010] Preferably, in step S2, the amount of α-amylase used is 0.5 wt% of the total amount of crushed highland barley rice.

[0011] Preferably, in step S3, the concentration of the ethanol solution is 60 wt %.

[0012] Preferably, in step S4, the temperature is 50°C.

[0013] Preferably, in step S4, the purification time is 4 hours.

[0014] Preferably, in step S4, the solid-liquid ratio is 1:80.

[0015] Preferably, in step S4, the amount of α-amylase used is 0.2 wt% of the total amount of the crushed β-glucan crude extract.

[0016] By adopting the technical scheme, the highland barley rice is first crushed to 80 meshes for subsequent processing, then only alpha-amylase is used in the extraction and enzymolysis process, the temperature, time, solid-liquid ratio, number of times and alpha-amylase dosage are strictly set, and the extraction reaction and the enzymolysis reaction are carried out at the same time, thereby saving time and ensuring the quality of extraction and enzymolysis. The highland barley beta-glucan in the beta-glucan crude extract has a high content, a mixed solution containing a large amount of beta-glucan crude extract is obtained, the mixed solution is allowed to stand for a period of time to obtain supernatant, the supernatant is filtered, two-stage filtration is carried out by connecting a plate and frame centrifuge and a tubular centrifuge in series, the cost is low, and the filtration efficiency is improved. The filtration efficiency is improved. Subsequently, concentration is carried out, and the concentration is stopped when the volume of the solution is reduced to 1 / 3 of the original volume. Ethanol is added to the system until the concentration of ethanol in the system reaches a certain value, and alcohol precipitation is carried out to obtain a beta-glucan crude extract suspension. Subsequently, filtration is carried out to obtain solid material (wet precipitate), which is then placed in a vacuum environment for drying and crushing to 50 meshes to obtain beta-glucan crude extract. Subsequently, the beta-glucan crude extract is purified, the temperature, solid-liquid ratio, time and alpha-amylase dosage are strictly controlled, and alpha-amylase is still used to treat the crude extract. Unlike the existing purification method which uses a large amount of ethanol, the present application uses alpha-amylase, which significantly reduces the amount of ethanol used in the overall process. After purification, the alpha-amylase is inactivated by heating, and then the precipitate is collected and dried in a vacuum environment to obtain highland barley beta-glucan.

[0017] In summary, the present application has the following beneficial technical effects: the preparation process of the present application has low cost, simple operation, and is convenient for batch production. The purity of the highland barley beta-glucan obtained finally can reach 80.35%, which has made a major progress in reducing cost and increasing efficiency, and has the dual advantages of large-scale operation and good product quality. DETAILED DESCRIPTION

[0018] The present application will be further described in detail below in combination with examples and comparative examples.

[0019] An industrial-grade continuous preparation process for efficiently producing high-purity highland barley beta-glucan includes the following steps: S1, crushing: crushing highland barley rice; S2, extraction and enzymolysis: under the conditions of a temperature of 65-85℃, an extraction time of 2-6h, and a solid-liquid ratio of 1: (12-20), alpha-amylase solution is used to extract and enzymolyze the crushed highland barley rice in step S1 to obtain a mixed solution. The amount of alpha-amylase in the alpha-amylase solution is 0.4-1.0wt% of the total amount of the crushed highland barley rice, and the number of extraction and enzymolysis is 2-3 times. S3, alcohol precipitation: the mixed solution obtained in step S2 is allowed to stand to obtain a supernatant, which is filtered and concentrated, and alcohol precipitation is performed using a 40-60 wt% ethanol solution to obtain a suspension of crude β-glucan extract, which is then filtered to obtain a solid substance, which is dried and crushed to obtain a crude β-glucan extract; S4, purification: Under the conditions of a temperature of 30-50°C, a purification time of 2-12 hours, and a solid-liquid ratio of 1:(60-90), the crude β-glucan extract in step S3 is purified using an α-amylase solution, filtered to obtain a solid substance, dried, and crushed to obtain highland barley β-glucan, wherein the amount of α-amylase in the α-amylase solution is 0.15-0.25wt% of the total amount of the crude β-glucan extract.

[0020] In a preferred embodiment, in step S2, the temperature is 75°C.

[0021] In a preferred embodiment, in step S2, the extraction time is 2 hours.

[0022] In a preferred embodiment, in step S2, the solid-liquid ratio is 1:14.

[0023] In a preferred embodiment, in step S2, the amount of α-amylase used is 0.5 wt % of the total amount of crushed highland barley rice.

[0024] In a preferred embodiment, in step S3, the concentration of the ethanol solution is 60 wt %.

[0025] In a preferred embodiment, in step S4, the temperature is 50°C.

[0026] In a preferred embodiment, in step S4, the purification time is 4 hours.

[0027] In a preferred embodiment, in step S4, the solid-liquid ratio is 1:80.

[0028] In a preferred embodiment, in step S4, the amount of α-amylase used is 0.2 wt % of the total amount of the crushed β-glucan crude extract.

[0029] Example 1 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan comprises the following steps: S1, pulverizing: highland barley rice is put into pulverizer and pulverized, passes 80 mesh sieve, weighs record total amount to 50kg, standby; S2, extraction and enzymolysis: whole highland barley rice, 250g α-amylase and 700L water after pulverizing in step S1 are blended, and drop into the extraction tank with a capacity of 2 tons, and the controlled temperature is 75 DEG C, and extraction and enzymolysis are carried out for 2h, and centrifugation is subsequently performed, and water is added according to solid-to-liquid ratio 1:14 after collecting the filter residue. Continuing to extract and enzymolysis time is 2h, and 2 extractions and enzymolysis are achieved; S3, alcohol precipitation: the mixed solution obtained in step S2 is allowed to stand to obtain a supernatant, and plate and frame centrifugation and tubular centrifugation are used in series for two-stage filtration. The mixture is concentrated by heating and concentrated until 1 / 3 of the original volume is stopped, and the mixture is put into an explosion-proof alcohol precipitation tank. 95wt% of edible ethanol is slowly added to the tank. Precipitation and aggregation gradually occur in the system. When the ethanol concentration in the system reaches 60wt%, a large amount of precipitate is accumulated in the system. After standing, the precipitate all tends to the bottom of the explosion-proof alcohol precipitation tank and settles. When no more precipitate falls at the bottom of the explosion-proof alcohol precipitation tank, the alcohol precipitation is terminated to obtain a crude β-glucan extract suspension, which is then filtered to collect the wet precipitate to obtain a solid substance, which is dried under a vacuum environment at 60°C, put into a grinder for pulverization, and sieved through a 50-mesh sieve to obtain a crude β-glucan extract; S4, purification: get the β-glucan crude extract 10kg that step S3 obtains, blend with 20g α-amylase and 800L water, drop into 2 tons of purification tanks with a capacity of dropping, and controlled temperature is 50 ℃, carry out purification, the time is 4h, adopt plate and frame centrifugation, tubular centrifugation series connection to carry out two-stage filtration after finishing, collect wet precipitation, obtain solid matter, be dried under 60 ℃ vacuum environment in temperature, drop into pulverizer and pulverize, cross 80 mesh sieves, obtain highland barley β-glucan.

[0030] Example 2 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S2, the temperature is controlled at 65° C., and the rest is the same as Example 1.

[0031] Example 3 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S2, the temperature is controlled at 85° C., and the rest is the same as Example 1.

[0032] Example 4 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S2, the extraction time is controlled to 4 hours, and the rest is the same as Example 1.

[0033] Example 5 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from example 1 in that in step S2, the extraction time is controlled to be 6 h, and the rest is the same as example 1.

[0034] Example 6 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from example 1 in that in step S2, the entire highland barley rice after crushing in step S1, 250 g of α-amylase and 600 L of water are blended, and the solid-liquid ratio is controlled to be 1:12, and the rest is the same as example 1.

[0035] Example 7 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from example 1 in that in step S2, the entire highland barley rice after crushing in step S1, 250 g of α-amylase and 800 L of water are blended, and the solid-liquid ratio is controlled to be 1:16, and the rest is the same as example 1.

[0036] Example 8 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from example 1 in that in step S2, the entire highland barley rice after crushing in step S1, 250 g of α-amylase and 900 L of water are blended, and the solid-liquid ratio is controlled to be 1:18, and the rest is the same as example 1.

[0037] Example 9 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from example 1 in that in step S2, the entire highland barley rice after crushing in step S1, 250 g of α-amylase and 1000 L of water are blended, and the solid-liquid ratio is controlled to be 1:20, and the rest is the same as example 1.

[0038] Example 10 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from example 1 in that in step S2, the entire highland barley rice after crushing in step S1, 200 g of α-amylase and 700 L of water are blended, and the amount of α-amylase is controlled to be 0.4 wt% of the total amount of the crushed highland barley rice, and the rest is the same as example 1.

[0039] Example 11 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from example 1 in that in step S2, the entire highland barley rice after crushing in step S1, 400 g of α-amylase and 700 L of water are blended, and the amount of α-amylase is controlled to be 0.8 wt% of the total amount of the crushed highland barley rice, and the rest is the same as example 1.

[0040] Example 12 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S2, all the highland barley rice, 500g α-amylase and 700L water after the pulverization in step S1 are blended, and the α-amylase amount is controlled to be 1.0wt% of the total amount of the highland barley rice after the pulverization, and the rest are the same as in Example 1.

[0041] Example 13 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S3, 95 wt% of edible ethanol is added to the tank until the ethanol concentration in the system reaches 40 wt%. The rest is the same as Example 1.

[0042] Example 14 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S4, the temperature is controlled at 30° C., and the rest is the same as Example 1.

[0043] Example 15 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S4, the temperature is controlled at 40° C., and the rest is the same as Example 1.

[0044] Example 16 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S4, the purification time is controlled to be 2 hours, and the rest are the same as Example 1.

[0045] Example 17 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S4, the purification time is controlled to 6 hours, and the rest is the same as Example 1.

[0046] Example 18 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S4, the purification time is controlled to 8 hours, and the rest is the same as Example 1.

[0047] Example 19 An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan is disclosed, which differs from Example 1 in that: in step S4, the purification time is controlled to 12 hours, and the rest is the same as Example 1.

[0048] Example 20 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from Example 1 in that in step S4, 10 kg of the β-glucan crude extract obtained in step S3 is blended with 20 g of α-amylase and 600 L of water, the solid-liquid ratio is controlled to be 1:60, and the rest is the same as in Example 1.

[0049] Example 21 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from Example 1 in that in step S4, 10 kg of the β-glucan crude extract obtained in step S3 is blended with 20 g of α-amylase and 700 L of water, the solid-liquid ratio is controlled to be 1:70, and the rest is the same as in Example 1.

[0050] Example 22 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from Example 1 in that in step S4, 10 kg of the β-glucan crude extract obtained in step S3 is blended with 20 g of α-amylase and 900 L of water, the solid-liquid ratio is controlled to be 1:90, and the rest is the same as in Example 1.

[0051] Example 23 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from Example 1 in that in step S4, 10 kg of the β-glucan crude extract obtained in step S3 is blended with 15 g of α-amylase and 800 L of water, the amount of α-amylase is controlled to be 0.15 wt% of the total amount of the β-glucan crude extract after crushing, and the rest is the same as in Example 1.

[0052] Example 24 An industrial continuous preparation process for efficiently producing high-purity highland barley β-glucan, which is different from Example 1 in that in step S4, 10 kg of the β-glucan crude extract obtained in step S3 is blended with 25 g of α-amylase and 800 L of water, the amount of α-amylase is controlled to be 0.25 wt% of the total amount of the β-glucan crude extract after crushing, and the rest is the same as in Example 1.

[0053] Comparative Example 1 50 kg of highland barley rice was reflux extracted with 80 wt % ethanol solution (solid-liquid volume ratio of 1:15) for 2 h, the precipitate was collected, dried, adjusted to pH = 8, and stirred and extracted at 65 ° C for 2 h, centrifuged at 4000 r / min for 15 min, the supernatant was adjusted to pH = 4.5 with 2 mol / L HCl solution, α-amylase was added according to the solid-liquid volume ratio of 1:10, and centrifuged at 4000 r / min for 20 min. The temperature of the supernatant was adjusted to 60 ° C, and 95 wt % ethanol was added at a volume ratio of 1:2 under a pressure of 0.09 MPa. The mixture was centrifuged at 4000 r / min for 20 min to obtain highland barley β-glucan.

[0054] Comparative Example 2 50 kg of highland barley rice was pretreated with 75 wt% ethanol solution (solid-liquid volume ratio 1:15), then hot water extracted twice in water at 80°C, precipitated with alcohol, and freeze-dried to obtain highland barley β-glucan.

[0055] Comparative Example 3 The highland barley grains were removed from impurities, washed, crushed, and sieved through a 40-mesh sieve. They were gelatinized in a water bath at a solid-liquid ratio of 1:6 and a temperature of 90°C. After 2 hours, α-amylase was added for enzymolysis for 30 minutes, and the filtrate was filtered (40 mesh) to obtain a filtrate. The filter residue was further gelatinized in a water bath at a solid-liquid ratio of 1:3 and a temperature of 90°C. After 2 hours, the filtrate was filtered (40 mesh) to obtain a second filtrate. The filter residue was further gelatinized in a water bath at a solid-liquid ratio of 1:2 and a temperature of 90°C. After 2 hours, the third filtrate was obtained. The filtrates were combined and centrifuged at a speed of 4000 r / min for 10 minutes to obtain a supernatant. 10% hydrogen peroxide was added for decolorization. After complete decolorization, 2 times the volume of ethanol was added for precipitation overnight, and the precipitate was filtered again (40 mesh). The precipitate was centrifuged at a speed of 4000 r / min for 10 minutes to obtain a precipitate, which was vacuum dried with silica to obtain highland barley β-glucan.

[0056] Performance testing The β-glucan content of the product (crude β-glucan extract) obtained in step S3 and the final product (highland barley β-glucan) obtained in step S4 in the examples and comparative examples of the present application was detected and recorded as the enzymatic extraction content and the purification content, respectively. The detection kit used was a Megazyme mixed β-glucan detection kit, and the results are recorded in Table 1.

[0057] Table 1 Data record table

[0058] Data Analysis: As can be seen from Table 1, in the preparation process of Example 1, the enzymatic extraction content can reach 45.89%, and the purified content can reach 80.35%. In the preparation process of Comparative Example 1, the purified content is 74.31%, and HCl solution is also used in the process flow, which is inconvenient to operate and relatively complicated. In the preparation process of Comparative Example 2, the purified content is 76.94%, and in the preparation process of Comparative Example 3, not only the purified content is extremely low, but also a gelatinization step is included, and the process flow is complicated. It can be seen that the preparation process of the present application has low cost, is easy to operate, and is easy to carry out batch production, and the purity of the highland barley β-glucan finally obtained can reach up to 80.35%, which has achieved significant progress in cost reduction and efficiency improvement, and has the dual advantages of large-scale operation and good product quality.

[0059] The embodiments of this specific implementation method are all preferred embodiments of the present application and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. An industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan, characterized in that: The following steps are involved: S1, crushing: crushing highland barley rice; S2, extraction and enzymolysis: under the conditions of a temperature of 65-85°C, an extraction time of 2-6h, and a solid-liquid ratio of 1:(12-20), the highland barley rice after the crushing in the step S1 is extracted and enzymolyzed using an α-amylase solution to obtain a mixed solution, wherein the amount of α-amylase in the α-amylase solution is 0.4-1.0wt% of the total amount of the crushed highland barley rice, and the number of extraction and enzymolysis is 2-3 times; S3, alcohol precipitation: the mixed solution obtained in step S2 is allowed to stand to obtain a supernatant, which is filtered and concentrated, and alcohol precipitation is performed using a 40-60 wt% ethanol solution to obtain a suspension of crude β-glucan extract, which is then filtered to obtain a solid substance, which is dried and crushed to obtain a crude β-glucan extract; S4, purification: Under the conditions of a temperature of 30-50°C, a purification time of 2-12 hours, and a solid-liquid ratio of 1:(60-90), the crude β-glucan extract in step S3 is purified using an α-amylase solution, filtered to obtain a solid substance, dried, and crushed to obtain highland barley β-glucan, wherein the amount of α-amylase in the α-amylase solution is 0.15-0.25wt% of the total amount of the crude β-glucan extract.

2. The industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan according to claim 1, characterized in that: In step S2, the temperature is 75°C.

3. The industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan according to claim 1, characterized in that: In step S2, the extraction time is 2 hours.

4. The industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan according to claim 1, characterized in that: In step S2, the solid-liquid ratio is 1:

14.

5. The industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan according to claim 1, characterized in that: In the step S2, the amount of α-amylase used is 0.5 wt% of the total amount of the crushed highland barley rice.

6. The industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan according to claim 1, characterized in that: In step S3, the concentration of the ethanol solution is 60 wt %.

7. The industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan according to claim 1, characterized in that: In step S4, the temperature is 50°C.

8. The industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan according to claim 1, characterized in that: In step S4, the purification time is 4 hours.

9. The industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan according to claim 1, characterized in that: In step S4, the solid-liquid ratio is 1:

80.

10. The industrial-grade continuous preparation process for efficiently producing high-purity highland barley β-glucan according to claim 1, characterized in that: In step S4, the amount of α-amylase used is 0.2 wt % of the total amount of the crushed β-glucan crude extract.

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