Detection equipment for bladder cancer metabonomics markers

Abstract

The invention relates to the technical field of cell detection equipment, in particular to bladder cancer metabonomics marker detection equipment which comprises a microplate reader, a mobile station and a pore plate placed in the mobile station, and further comprises a bubble remover arranged on the outer wall of the microplate reader, the bubble remover comprises a fixed part, a pair of transmission parts, and a knocking part and an air blowing part which are arranged in the fixed part. According to the bladder cancer metabonomics marker detection equipment, through the mechanical linkage design of the moving table and the bubble remover, a toothed plate is engaged with a gear, a driving plate drives a bent plate to move up and down, a rapping rod is driven to knock a pore plate, synchronous airflow injection of an air blowing part is combined, a piston plate is pushed in a second sliding groove by utilizing a convex block, and a spray head blows air directionally; bubbles in holes are automatically removed before detection of the microplate reader, optical interference is eliminated through double bubble removal of knocking vibration and airflow impact, the accuracy of detection numerical values is ensured, and the detection process is simplified.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell detection equipment, in particular to a detection equipment for bladder cancer metabolomics markers. BACKGROUND

[0002] Existing data shows that high expression of PSMB5 is an independent risk prognostic factor for OS of bladder cancer patients, in order to clarify the influence of PSMB5 on the phenotype of bladder urothelial carcinoma cells, the CCK8 method is used to detect the proliferation of bladder urothelial carcinoma cells, and the detection is usually based on enzyme-linked immunosorbent assay (ELISA), colorimetric method or fluorescence method, etc., and is carried out in a microplate, and an enzyme label instrument can accurately detect the weak color change in the microhole produced by the marker-enzyme reaction, so as to realize accurate quantitative analysis of trace metabolic markers, and is a key equipment in the detection.

[0003] The patent with the application number CN202422147576.2 discloses a novel enzyme label instrument based on a grating module and a filter module, which comprises an electric enzyme label instrument body, a grating module, a filter module, a motor speed reduction mechanism, and further comprises a detection mechanism, a first detection control circuit and a second detection control circuit; the motor speed reduction mechanism is installed at the rear side end in the detection bin, and the front side end of the rotating shaft of the motor speed reduction mechanism is provided with a supporting rod and a connecting rod; the grating module and the filter module are installed together at the front side two ends of the connecting rod at the rear side end; the staff is facilitated, and the equipment fund investment of the relevant departments is saved.

[0004] The existing enzyme label instrument lacks an automatic bubble removal structure during detection, usually needs manual bubble removal, has low operation efficiency and insufficient removal rate, and is difficult to remove stubborn bubbles adhered to the hole wall, so as to cause light path interference and cause deviation of OD value; at the same time, external centrifugation or pipetting operation forces the cells to separate from the constant temperature environment, causes degradation of formazan dye, and significantly magnifies the data fluctuation in the group; in addition, the fragmented bubble removal process prolongs the exposure time of the sample, and seriously restricts the statistical efficiency of the bladder cancer cell phenotype research.

[0005] In view of this, a detection equipment for bladder cancer metabolomics markers is provided. SUMMARY

[0006] The purpose of the present application is to provide a detection equipment for bladder cancer metabolomics markers to solve the problems in the background art.

[0007] To achieve the above purpose, the present application provides the following technical scheme: A detection equipment for bladder cancer metabolomics markers, comprising an enzyme label instrument, a moving table and a hole plate placed inside, further comprising a bubble remover provided on the outer wall of the enzyme label instrument, the bubble remover comprising a fixed part, a pair of transmission parts, and a knocking part and a blowing part provided inside the fixed part. The moving table comprises a table plate and toothed plates arranged on the left and right sides of the table plate; The transmission part comprises a dial, a gear arranged at the end of the central shaft of the dial, and a dial rod arranged on the outer wall of the dial close to the edge; When the table plate is retracted into the enzyme marker, the toothed plates engage the gear to drive the dial to rotate; The knocking part comprises a pair of bent plates, a lifting plate arranged on the top surface of the pair of bent plates, a plurality of knocking rods distributed on the bottom surface of the lifting plate, and a protrusion arranged on the front wall of the lifting plate. When the dial rotates, the dial rod is in contact with the horizontal plate at the bottom of the bent plate, which drives the bent plate to move upward, and after the dial rod is separated from the bent plate, the bent plate falls back to the original position, the knocking rod knocks the surface of the hole plate, and the gas bubble is separated from the hole wall and floats up to be broken; The air blowing part comprises a frame plate, a piston plate moving with the frame plate, a gas cylinder sleeved outside the piston plate, and a nozzle provided with a plurality of gas pipes at the bottom, and a second sliding groove is formed in the inside of the frame plate. When the lifting plate moves up and down, the protrusion moves in the second sliding groove, which drives the frame plate to move left and right in the gas cylinder, and the gas is sent into the nozzle through the pipeline and blown into the hole of the hole plate through the gas pipes to eliminate the unbroken gas bubbles.

[0008] In the technical scheme of the present application, the table plate is connected with the electric push rod inside the enzyme marker for controlling the movement of the table plate, a placing groove for placing the hole plate is formed on the top surface of the table plate, a plurality of recesses for providing a falling interval for the internal structure of the air blowing part are formed on the left and right sides of the placing groove on the top surface of the table plate, and the toothed plate is fixedly connected to the outer wall of the table plate by screws.

[0009] The design of the recesses and the toothed plate enables the moving table to generate power when moving as a whole, which can drive the internal structure of the bubble remover to move to eliminate the gas bubbles in the holes of the hole plate.

[0010] In the technical scheme of the present application, the fixed part comprises a fixed box, an upper partition plate connected to the inner wall of the fixed box, and an inner partition plate fixedly connected to the bottom surface of the upper partition plate on the left and right ends.

[0011] In the technical scheme of the present application, the left and right ends of the upper partition plate are provided with through grooves penetrating upward and downward, and the outer wall of the inner partition plate is provided with a rotating groove, and the inner wall of the inner partition plate is provided with a first sliding groove for providing a sliding interval for the internal structure of the air blowing part.

[0012] The design of the upper partition plate and the inner partition plate separates the internal space of the fixed box.

[0013] The dial is rotationally connected to the inside of the rotating groove, and the end center shaft extends to the inside of the inner partition plate.

[0014] The setting transmits the power generated when the mobile station is moved by the gear engaging with the toothed plate.

[0015] In the technical scheme, the end of the bottom transverse plate of the bent plate extends to the center of the dial, the bottom surface is above the dial lever, the longitudinal plate body is slidingly connected to the inside of the first sliding groove and the end is clamped to the bottom surface of the lifting plate, and the knocking lever and the protruding block are hot melt connected to the outer wall of the lifting plate.

[0016] In the technical scheme, the top surface of the bent plate is also clamped and fixed with a plurality of regularly distributed telescopic rods and springs sleeved outside the telescopic rods, the top end of the telescopic rod is clamped and fixed to the inside top surface of the fixed box, and the spring provides elastic force to push the lifting plate to move downward.

[0017] The above setting eliminates the bubbles on the hole wall through the physical knocking mode of the knocking lever knocking the hole plate surface, reduces the link of manual bubble elimination, and simplifies the detection process.

[0018] In the technical scheme, the frame plate is slidingly connected to the top surface of the upper partition plate, a round rod is clamped and fixed between the end of the frame plate and the piston plate, and the piston plate is slidingly connected to the inside of the air cylinder.

[0019] In the technical scheme, the air cylinder is fixedly connected to the top surface of the upper partition plate through a screw, the upper and lower ends of the air cylinder end are respectively provided with an air inlet valve and an air outlet valve, a hose is clamped outside the air outlet valve of the air cylinder, and the other end of the hose is clamped and fixed with the spray head.

[0020] In the technical scheme, the lower side of the spray head is also provided with a square plate slidingly connected to the inside of the first sliding groove, the bottom surface of the two ends of the square plate is clamped with a lower protruding rod, a plurality of air nozzles sleeved in the air pipe at the bottom of the spray head are clamped and fixed in the inside of the square plate, and the end of the lower protruding rod is provided with a chamfer.

[0021] The above setting makes the airflow act on the bubble aggregation area at a close distance, realizes local strengthening and removal of stubborn bubbles, improves the removal rate of adhesive bubbles, and provides a reliable basis for statistical analysis of the PSMB5 phenotype influence.

[0022] Compared with the prior art, the present application has the following advantages: 1. The bladder cancer metabolomics marker detection device, through the mechanical linkage design of the mobile station and the bubble remover, the gear meshing gear, the driving of the dial plate, the up and down movement of the bending plate, the knocking of the knocking rod on the hole plate, the synchronous airflow injection of the air blowing part, the use of the protruding block in the second sliding groove to push the piston plate, the directional blowing of the nozzle, the automatic removal of the bubbles in the hole before the detection of the enzyme label instrument, the double bubble removal of the knocking vibration and the airflow impact, the elimination of the optical interference, the accuracy of the detection value, and the simplification of the detection process.

[0023] 2. The bladder cancer metabolomics marker detection device, the dynamic positioning of the air nozzle is triggered by the displacement of the table plate, when the groove moves to the lower side of the lower convex rod, the square plate slides down along the first sliding groove, the air nozzle is inserted into the hole, the airflow acts on the bubble aggregation area at close range, the stubborn bubbles are removed, the elimination rate of the adhesive bubbles is improved, the uniformity of the bladder cancer cell proliferation data in the high serum environment is ensured, and a reliable foundation is provided for the statistical analysis of the PSMB5 phenotype influence. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of the present application; Figure 2 It is a schematic diagram of the partial structure of the present application; Figure 3 It is a schematic diagram of the structure of the mobile station in the present application; Figure 4 It is a schematic diagram of the structure of the bubble remover in the present application; Figure 5 It is a schematic diagram of the structure of the bubble remover in the present application; Figure 6 It is a schematic diagram of the structure of the bubble remover in the present application; Figure 7 It is a schematic diagram of the structure of the fixed part in the present application; Figure 8 It is a schematic diagram of the structure of the transmission part in the present application; Figure 9 It is a schematic diagram of the structure of the knocking part in the present application; Figure 10 It is a schematic diagram of the structure of the air blowing part in the present application; Figure 11 It is a schematic diagram of the structure of the air blowing part in the present application; Explanation of reference signs: 100, enzyme label instrument; 200, mobile station; 210, table plate; 211, placement groove; 212, groove; 220, gear plate; 300, hole plate; 400, bubble remover; 410, fixing part; 411, fixing box; 412, upper partition; 4120, through slot; 413, inner partition; 4130, rotating slot; 4131, first sliding slot; 420, transmission part; 421, dial; 422, gear; 423, lever; 430, knocking part; 431, bent plate; 432, lifting plate; 433, knocking rod; 434, protrusion; 435, telescopic rod; 436, spring; 440, air blowing part; 441, frame plate; 4410, second sliding slot; 442, round rod; 443, piston plate; 444, air cylinder; 445, hose; 446, nozzle; 447, square plate; 448, lower protrusion; 449, air nozzle. DETAILED DESCRIPTION

[0025] The technical solutions in the present application will be described clearly and completely below in conjunction with the drawings in the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0026] Please refer to Figures 1-3 The present embodiment provides a technical solution as shown in the drawings: The detection device for bladder cancer metabolomics markers comprises an enzyme label instrument 100, a mobile station 200, and a well plate 300 placed inside, further comprises a bubble remover 400 arranged on the outer wall of the enzyme label instrument 100, the bubble remover 400 comprises a fixing part 410, a pair of transmission parts 420, and a knocking part 430 and an air blowing part 440 arranged inside the fixing part 410. Specifically, the mobile station 200 comprises a platform 210 and a tooth plate 220 arranged on the left and right sides thereof.

[0027] Further, the platform 210 is clamped and fixed with an electric push rod inside the enzyme label instrument 100 for controlling the movement thereof, a placing groove 211 for placing the well plate 300 is formed on the top surface of the platform 210, a plurality of recesses 212 for providing falling intervals for the internal structure of the air blowing part 440 are formed on the left and right sides of the placing groove 211 on the top surface of the platform 210, and the tooth plate 220 is fixedly connected to the outer wall of the platform 210 by screws.

[0028] Further, after the well plate 300 is completed, it is placed inside the placing groove 211 of the platform 210, the enzyme label instrument 100 is started, the parameters are set, and then the mobile station 200 is recovered to detect the well plate 300, and this setting makes the mobile station 200 move as a whole to generate power and drive the internal structure of the bubble remover 400 to move, so as to eliminate the air bubbles in the holes of the well plate 300.

[0029] Referring to Figures 2-7 In this embodiment, the fixing part 410 includes a fixing box 411, an upper partition plate 412 clamped on the inner wall of the fixing box 411, and an inner partition plate 413 clamped on the bottom surface of the upper partition plate 412.

[0030] Specifically, the upper partition plate 412 is provided with a through slot 4120 penetrating from top to bottom at both ends thereof, and the outer wall of the inner partition plate 413 is provided with a rotating slot 4130, and the inner wall of the inner partition plate 413 is provided with a first sliding slot 4131 for providing a sliding interval for the internal structure of the air blowing part 440.

[0031] Further, the fixing box 411 is used to provide a placement interval for the transmission part 420, the knocking part 430 and the air blowing part 440, and the internal space of the fixing box 411 is separated by the design of the upper partition plate 412 and the inner partition plate 413.

[0032] Referring to Figures 2-6 In this embodiment, the transmission part 420 includes a dial 421, a gear 422 arranged at the end of the central shaft of the dial 421, and a dial rod 423 arranged on the outer wall of the dial 421 close to the edge, and when the platform 210 is retracted into the enzyme marker 100, the toothed plate 220 engages the gear 422 to drive the dial 421 to rotate.

[0033] Specifically, the dial 421 is rotatably connected to the inside of the rotating slot 4130, and the end of the central shaft thereof extends to the inside of the inner partition plate 413, the gear 422 is clamped and fixed to the end of the central shaft of the dial 421, and the dial rod 423 is integrally formed with the dial 421.

[0034] Further, during the movement of the moving platform 200, the toothed plate 220 engages the gear 422 to drive the dial 421 to rotate, and the position of the dial rod 423 is continuously adjusted, and the power generated during the movement of the moving platform 200 is transmitted by the engagement of the toothed plate 220 and the gear 422.

[0035] Referring to Figures 2-9 In this embodiment, the knocking part 430 includes a pair of bent plates 431, a lifting plate 432 arranged on the top surface of the pair of bent plates 431, a plurality of knocking rods 433 distributed on the bottom surface of the lifting plate 432, and a protrusion 434 arranged on the front wall of the lifting plate 432, when the dial 421 rotates and the dial rod 423 contacts the bottom horizontal plate of the bent plate 431, the bent plate 431 is driven to move upward, and after the dial rod 423 is separated from the bent plate 431, the bent plate 431 falls back to reset, the knocking rod 433 knocks the plate surface of the hole plate 300, and the bubbles are separated from the hole wall and float to break.

[0036] Specifically, the bottom transverse plate end of the bent plate 431 extends to the center of the dial 421, the bottom surface is above the knocking lever 423, the longitudinal plate body is slidingly connected inside the first sliding groove 4131 and the end is clamped on the bottom surface of the lifting plate 432, the knocking lever 433 and the protrusion 434 are both hot melt connected on the outer wall of the lifting plate 432.

[0037] Further, a plurality of regularly distributed telescopic rods 435 are clamped and fixed on the top surface of the bent plate 431, and springs 436 sleeved outside the telescopic rods 435 are further provided, the top end of the telescopic rod 435 is clamped and fixed on the inner top surface of the fixed box 411, and the spring 436 provides elastic force to push the lifting plate 432 to move downward.

[0038] Further, when the dial 421 rotates and the knocking lever 423 is in contact with the bottom transverse plate of the bent plate 431, the knocking lever 423 drives the bent plate 431 to move upward, and after the knocking lever 423 is separated from the bent plate 431, the spring 436 is pushed to reset the bent plate 431 to fall downward by the elastic force of the spring 436, and the knocking lever 433 knocks the plate surface of the hole plate 300 to make the bubbles separate from the hole wall and float upward to break, which eliminates the bubbles on the hole wall by knocking the plate surface of the hole plate 300 by the knocking lever 433, reduces the link of manual elimination of bubbles, and simplifies the detection process.

[0039] Please refer to Figures 2-11 In the embodiment, the air blowing part 440 includes a frame plate 441, a piston plate 443 moving with the frame plate 441, an air cylinder 444 sleeved outside the piston plate 443, and a spray head 446 provided with a plurality of air pipes at the bottom, the inner part of the frame plate 441 is provided with a second sliding groove 4410, the lifting plate 432 moves up and down, the protrusion 434 moves in the second sliding groove 4410, drives the frame plate 441 to drive the piston plate 443 to move left and right in the air cylinder 444, sends the gas into the spray head 446 through the pipeline, and blows the gas into the holes of the hole plate 300 through the air pipes to eliminate the unbroken bubbles.

[0040] Specifically, the frame plate 441 is slidingly connected to the top surface of the upper partition plate 412, a round rod 442 is clamped and fixed between the end of the frame plate 441 and the piston plate 443, and the piston plate 443 is slidingly connected inside the air cylinder 444.

[0041] Further, the air cylinder 444 is fixedly connected to the top surface of the upper partition plate 412 by screws, the upper and lower ends of the end of the air cylinder 444 are respectively provided with an air inlet valve and an air outlet valve, the air cylinder 444 is clamped with a hose 445 outside the air outlet valve, and the other end of the hose 445 is clamped and fixed with the spray head 446.

[0042] Further, a square plate 447 slidingly connected inside the first sliding groove 4131 is further arranged below the spray head 446, lower protrusions 448 are clamped on the bottom surfaces of both ends of the square plate 447, a plurality of air nozzles 449 sleeved in the air pipes at the bottom of the spray head 446 are clamped and fixed inside the square plate 447, and a chamfer is arranged on the end of the lower protrusion 448.

[0043] Further, during the up and down movement of the lifting plate 432, the convex block 434 moves in the second sliding groove 4410, drives the frame plate 441 to drive the piston plate 443, moves left and right in the air cylinder 444 through the round rod 442, sends the gas into the spray head 446 through the hose 445, and blows the gas into the holes of the hole plate 300 through the gas pipes, so as to eliminate the unbroken bubbles, and at the same time, when the groove 212 on the table plate 210 moves to the lower side of the lower convex rod 448, the bottom end falls into the groove 212 under the action of gravity, drives the square plate 447 to slide downward in the first sliding groove 4131, and then makes the gas nozzles 449 deeply enter the holes of the hole plate 300, so that the gas flow is close to the bubbles, thereby ensuring the elimination of the bubbles, and the setting makes the gas flow act on the bubble gathering area at a close distance, realizes local strengthening and removal of stubborn bubbles, improves the elimination rate of the adhesive bubbles, and provides a reliable basis for statistical analysis of the PSMB5 phenotype influence.

[0044] The detection equipment for the bladder cancer metabolomics marker of the application, in use, in order to determine the influence of PSMB5 on the phenotype of bladder urothelial carcinoma cells, first, the 5637 cells and J82 cells in the logarithmic growth phase of the control group (without treatment), the empty group (transfected with empty vector lentivirus), and the sh-RNA group (transfected with PSMB5 shRNA lentivirus) are respectively digested (trypsin / EDTA) to prepare single-cell suspensions; Then, the cells are inoculated in a sterile 96-well plate, the cell concentration is adjusted to a suitable density with complete culture medium, and it is ensured that the cell densities of the groups in the same experiment must be consistent, and a blank control group hole (zero adjustment hole) is set, complete culture medium without cells is added to each hole for background absorbance deduction, the hole plate 300 is gently shaken to make the cells uniformly distributed, and group labels are made on the edge of the hole plate 300 cover; The hole plate 300 is placed in a 37°C, 5% CO2 incubator for about 12-24 hours to allow the cells to fully adhere and adapt to the environment; Subsequently, in a sterile EP tube or reagent bottle, CCK8 reagent is mixed with fresh complete culture medium at a ratio of 1:9, the hole plate 300 is taken out of the incubator, the old culture medium in each hole is carefully sucked and discarded with a pipette, fresh complete culture medium is added to the experimental group holes and the blank control group holes to ensure that the cells are immersed, then the premixed CCK8 working solution is added to each hole; Then, the hole plate 300 is taken out and placed in the placing groove 211 of the table plate 210, the enzyme marker 100 is started, the parameters are set, and then the moving table 200 is recovered to detect the hole plate 300; In the process of moving of the mobile station 200, the toothed plate 220 engages the gear 422 to drive the dial plate 421 to rotate, the dial rod 423 is in contact with the bottom transverse plate of the bent plate 431, and the bent plate 431 is driven to move upwards, and after being separated, the spring 436 in the retracted state pushes the bent plate 431 to fall and reset by the elastic force of itself, the knocking rod 433 knocks the hole plate 300, the bubble is separated from the hole wall and floats to break; In the process of moving of the lifting plate 432, the convex block 434 moves in the second sliding groove 4410 to drive the frame plate 441 to drive the piston plate 443 to move left and right in the air cylinder 444 through the round rod 442, to send the gas into the spray head 446 through the hose 445, and to blow the gas into the hole of the hole plate 300 through the gas pipes, so as to eliminate the unbroken bubble; At the same time, when the recess 212 on the platform 210 moves to the lower side of the lower convex rod 448, the bottom end falls into the recess 212 under the action of gravity, drives the square plate 447 to slide downwards in the first sliding groove 4131, and then drives the gas nozzles 449 to enter the hole of the hole plate 300, so that the gas flow is close to the bubble, thereby ensuring the elimination of the bubble. After the detection of the enzyme label instrument 100 is completed, statistical software is used for comparison between multiple groups, and statistical analysis is performed on the detection data.

[0045] The foregoing description of specific exemplary embodiments of the application is intended to be illustrative only and is not intended to limit the application to the precise forms described. Many modifications and variations are possible in light of the above teachings without departing from the spirit or essential characteristics of the application. The exemplary embodiments were chosen and described in order to explain the principles of the application and its practical application and to allow others skilled in the art to understand the application for various exemplary embodiments with various modifications being suited to the particular use contemplated. The scope of the application is to be defined by the claims and their equivalents.

Claims

1. A device for detecting bladder cancer metabolomics markers, comprising an enzyme reader, a mobile platform, and a well plate placed therein, characterized in that: The apparatus further comprises a debubbler disposed on the outer wall of the microplate reader, wherein the debubbler comprises a fixing portion, a pair of transmission portions, and a knocking portion and an air blowing portion disposed inside the fixing portion; The movable platform includes a platform and tooth plates arranged on the left and right sides thereof; The transmission part includes a dial, a gear arranged at the end of the central axis thereof, and a lever arranged on the outer wall of the dial near the edge. When the platform is retracted into the inside of the microplate reader, the gear plate engages with the gear to drive the dial to rotate; The knocking part includes a pair of bending plates, a lifting plate arranged on the top surface of the pair of bending plates, a plurality of knocking rods distributed on the bottom surface of the lifting plate, and a protrusion arranged on the front wall of the lifting plate. When the dial rotates, the knocking rods come into contact with the horizontal plate at the bottom of the bending plate, driving it to move upward. After separation from it, the bending plate falls and resets, and the knocking rods knock the surface of the orifice plate, so that the bubbles break away from the orifice wall and float up and burst. The air blowing part includes a frame plate, a piston plate that moves with it, an air cylinder mounted outside the piston plate, and a nozzle with a plurality of air pipes at the bottom. A second slide groove is opened inside the frame plate, the lifting plate moves up and down, and the protrusion moves in the second slide groove, driving the frame plate to drive the piston plate to move left and right in the air cylinder, and the gas is sent into the nozzle through the pipeline and blown into the hole of the orifice plate by a plurality of air pipes to eliminate unbroken bubbles.

2. The bladder cancer metabolomics marker detection device according to claim 1, characterized in that: The table is fixedly connected to the electric push rod inside the microplate reader for controlling its movement. A placement groove for placing the orifice plate is provided on the top surface of the table. Several regularly distributed grooves are provided on the left and right sides of the placement groove to provide a falling range for the internal structure of the air blowing part. The tooth plate is fixedly connected to the outer wall of the table by screws.

3. The detection device for bladder cancer metabolomics markers according to claim 1, characterized in that: The fixing portion includes a fixing box, an upper partition board clamped on the inner wall of the fixing box, and inner partition boards clamped and fixed on the bottom surfaces of the left and right ends of the upper partition board.

4. The detection device for bladder cancer metabolomics markers according to claim 3, characterized in that: The left and right ends of the upper partition are both provided with vertically penetrating grooves, the outer wall of the inner partition is provided with a rotation groove, and the inner wall is provided with a first sliding groove for providing a sliding interval for the internal structure of the air blowing part.

5. The detection device for bladder cancer metabolomics markers according to claim 1, characterized in that: The dial is rotatably connected to the inside of the rotating groove, and the central axis of its end extends to the inner side of the inner partition. The gear is clamped and fixed at the end of the central axis of the dial, and the dial rod is integrally formed with the dial.

6. The detection device for bladder cancer metabolomics markers according to claim 1, characterized in that: The end of the bottom horizontal plate of the bent plate extends to the center of the dial, and the bottom surface is located above the dial rod. Its longitudinal plate body is slidably connected to the inside of the first slide groove and the end is clamped on the bottom surface of the lifting plate. The knock rod and the protrusion are both hot-melt connected to the outer wall of the lifting plate.

7. The detection device for bladder cancer metabolomics markers according to claim 1, characterized in that: A number of regularly distributed telescopic rods and springs sleeved on the outside of the telescopic rods are also clamped and fixed on the top surface of the bending plate. The top ends of the telescopic rods are clamped and fixed on the inner top surface of the fixed box. The elastic force provided by the springs pushes the lifting plate downward.

8. The detection device for bladder cancer metabolomics markers according to claim 1, characterized in that: The frame plate is slidably connected to the top surface of the upper partition plate, a round rod is clamped and fixed between the end of the frame plate and the piston plate, and the piston plate is slidably connected to the inside of the air cylinder.

9. The detection device for bladder cancer metabolomics markers according to claim 1, characterized in that: The air cylinder is fixedly connected to the top surface of the upper partition by screws, and an air inlet valve and an air outlet valve are respectively provided at the upper and lower ends of the air cylinder. A hose is clamped on the outside of the air outlet valve, and the other end of the hose is clamped and fixed to the nozzle.

10. The detection device for bladder cancer metabolomics markers according to claim 1, characterized in that: A square plate is provided below the nozzle and is slidably connected to the inside of the first slide groove. Lower protrusions are clamped on the bottom surfaces of both ends of the square plate. Several air nozzles arranged in the air pipe at the bottom of the nozzle are fixed on the inside of the square plate. The ends of the lower protrusions are chamfered.

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