Method for cultivating agriophyllum squarrosum sprouting vegetables rich in gamma-aminobutyric acid

Abstract

The invention relates to the technical field of cultivation of agriophyllum squarrosum sprouting vegetables, and discloses a cultivation method of agriophyllum squarrosum sprouting vegetables rich in gamma-aminobutyric acid, which comprises the following steps: selecting agriophyllum squarrosum seeds, soaking the agriophyllum squarrosum seeds with clear water, and then disinfecting the agriophyllum squarrosum seeds with a sodium hypochlorite solution to obtain treated agriophyllum squarrosum seeds; sowing the treated agriophyllum squarrosum seeds, covering the surfaces of the agriophyllum squarrosum seeds with a layer of thin gauze after sowing, and performing first-stage cultivation; second-stage cultivation is conducted after first-stage cultivation is finished, harvesting is conducted when the sprouting vegetables grow to 8-10 cm high, and the agriophyllum squarrosum sprouting vegetables rich in gamma-aminobutyric acid are obtained. Nutritional characteristics of agriophyllum squarrosum are fully utilized, and the cultivated agriophyllum squarrosum sprouting vegetables are rich in gamma-aminobutyric acid, protein, dietary fibers, vitamins and mineral substances, high in nutritional value and capable of meeting requirements of people for healthy food.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of Salicornia germination, in particular to a Salicornia germination method rich in gamma-aminobutyric acid. BACKGROUND

[0002] Salicornia (Agriophyllum squarrosum) is a typical drought-tolerant and saline-alkali-tolerant plant, widely distributed in arid and semi-arid regions. Its seeds contain rich nutrients, including protein, dietary fiber, unsaturated fatty acids, various vitamins and minerals, and have high nutritional value.

[0003] With the improvement of people's health consciousness, the demand for healthy food is increasing, and Salicornia and its derivative products have gradually entered the public eye. However, the traditional planting method of Salicornia is limited by environmental conditions, and the yield is low, and the utilization rate of the seeds after harvesting is not high, which fails to fully develop its potential value.

[0004] Sprouts are a kind of high-nutrition and short-cycle vegetables, which are deeply loved by consumers and have broad market prospects. However, as of now, there is no relevant report on the cultivation technology of Salicornia sprouts. Gamma-aminobutyric acid (GABA) is an important functional ingredient, which has many physiological functions such as lowering blood pressure, calming nerves, and improving sleep.

[0005] Therefore, a Salicornia sprout cultivation method rich in gamma-aminobutyric acid is provided, which has important economic and social value for fully utilizing Salicornia resources and meeting people's demand for healthy food. SUMMARY

[0006] In view of this, the present application provides a Salicornia sprout cultivation method rich in gamma-aminobutyric acid, which aims to solve at least one of the problems in the above background art.

[0007] The present application provides a Salicornia sprout cultivation method rich in gamma-aminobutyric acid, comprising the following steps:

[0008] Select Salicornia seeds, soak them with water, and then disinfect them with sodium hypochlorite solution to obtain treated Salicornia seeds;

[0009] The treated Salicornia seeds are sown in the culture medium, and a layer of gauze is covered on the surface after sowing for the first stage of cultivation;

[0010] The second stage of cultivation is carried out after the first stage of cultivation, and the sprouts are harvested when they grow to 8-10 cm high to obtain Salicornia sprouts rich in gamma-aminobutyric acid.

[0011] Preferably, the soaking time is 6-8 hours.

[0012] Preferably, the disinfection is performed by using 0.1% sodium hypochlorite solution to disinfect the seed of the sand millet for 10 minutes, and then rinsing with clean water.

[0013] Preferably, the concentration of the seed of the sand millet during sowing is 4-5 grains / cm 2

[0014] Preferably, the first stage cultivation is performed under the conditions of temperature 20-25 DEG C, humidity 80%-90%, and light shielding treatment.

[0015] Preferably, the second stage cultivation is performed under the conditions of temperature 15-25 DEG C, humidity 70%-80%, and sunlight irradiation treatment.

[0016] Preferably, the first stage cultivation and the second stage cultivation are both performed by spraying neutral pure water, with pH value controlled at 6.5-7.0, the spraying frequency is 2 days / time, and the spraying amount is 200-300 mL / m 2 .

[0017] Preferably, the culture medium is mixed by coconut husk, perlite and vermiculite at the ratio of 3:1:1.

[0018] Preferably, the seed of the sand millet is selected from the seeds with full grains and free of diseases and insect pests.

[0019] The application also provides the application of the sand millet sprout rich in gamma-aminobutyric acid in the cultivation of the sand millet sprout.

[0020] Compared with the prior art, the application has the advantages of:

[0021] (1) The sand millet sprout cultivated by the application is rich in gamma-aminobutyric acid, protein, dietary fiber, vitamins and minerals, and has high nutritional value, which can meet the demand of people for healthy food.

[0022] (2) The application adopts the soilless culture technology, and uses coconut husk, perlite and vermiculite at a specific ratio as the culture medium, so that soil pollution is avoided, and the hygiene safety of the sprout is improved.

[0023] (3) The application optimizes the cultivation environment, such as temperature, humidity, light and the like, and controls the water in a reasonable manner, so that the growth cycle of the sand millet sprout is shortened, the yield is improved, and the economic benefit is improved.

[0024] (4) The method of the application is simple and easy to understand, the parameters of each link are clear, the method is suitable for large-scale production, and has good popularization prospect. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Various other advantages and benefits will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiment below. The accompanying drawings are for illustration purposes only and are not to be considered as limiting the present invention. The same reference symbols are used throughout the drawings to represent the same components. In the drawings:

[0026] Figure 1 This is a flow chart of the method for cultivating the γ-aminobutyric acid-rich Salsop serrata sprouts of the present invention; DETAILED DESCRIPTION

[0027] Various exemplary embodiments of the present invention are now described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terms used in the present invention are only for describing specific embodiments and are not intended to limit the present invention.

[0028] In addition, for numerical ranges in the present invention, it is understood that each intervening value between the upper and lower limits of the range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated value or intervening value in the stated range is also included in the present invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

[0029] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.

[0030] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from the description of the invention. The description and examples are intended to be exemplary only.

[0031] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0032] The present invention provides a method for cultivating Salsopsis pilosula sprouts rich in gamma-aminobutyric acid, which is characterized by comprising the following steps:

[0033] Select the seed of the sand, soak it with clean water, and then disinfect it with sodium hypochlorite solution to obtain the treated sand seed;

[0034] The treated sand seed is sown in the culture medium, a layer of gauze is covered on the surface after sowing, and the first stage cultivation is carried out;

[0035] The second stage cultivation is carried out after the first stage cultivation, and the bud seedling is harvested when the bud seedling is 8-10 cm high, and the sandwort bud seedling rich in gamma-aminobutyric acid is obtained.

[0036] Select the seed of the sand, soak it with clean water, and then disinfect it with sodium hypochlorite solution to obtain the treated sand seed;

[0037] Select high-quality sand seed, the sand strain is one of Minqin, Wulanbuhe, Naiman, Alxang, Haolaihu, soak, screen and disinfect, to provide a good foundation for subsequent cultivation. Soaking can make the seed absorb water, which is beneficial to germination; screening can remove inferior seeds to ensure seed quality; disinfection can reduce the influence of diseases and pests.

[0038] In the application, the soaking time is preferably 6-8 hours.

[0039] In the application, the disinfection is specifically preferred as follows: the sand seed is disinfected with 0.1% sodium hypochlorite solution for 10 minutes, and then washed clean with clean water.

[0040] The treated sand seed is sown, and a layer of gauze is covered on the surface after sowing, and the first stage cultivation is carried out;

[0041] The second stage cultivation is carried out after the first stage cultivation, and the bud seedling is harvested when the bud seedling is 8-10 cm high, and the sandwort bud seedling rich in gamma-aminobutyric acid is obtained.

[0042] The treated seed is sown in the cultivation tray at a certain density, and the gauze with a reasonable covering thickness of about 0.5 cm is covered to create a suitable germination and growth environment. The temperature, humidity and light conditions at different growth stages are controlled to promote the healthy growth of the bud seedling. The shading treatment in the early stage of cultivation is beneficial to seed germination, and the light after germination can meet the needs of photosynthesis.

[0043] In the application, the concentration of the sand seed when sowing is preferably 4-5 grains / cm 2

[0044] In the application, the first stage cultivation is preferably carried out under the following conditions: temperature 20-25℃, humidity 80%-90%, shading treatment.

[0045] The first stage cultivation of the application corresponds to the initial stage of the cultivation of the sand millet seeds, and the time is 1-3 days.

[0046] In the application, the conditions of the second stage cultivation are preferably that the temperature is 15-25℃, the humidity is 70%-80%, and the sunlight irradiation treatment is provided.

[0047] The second stage cultivation of the application corresponds to the stage after the germination of the sand millet seeds, and the time is 4-7 days.

[0048] In the application, the first stage cultivation and the second stage cultivation are both sprayed with neutral pure water, the pH value is controlled to be 6.5-7.0, the spraying frequency is 2 days / time, and the spraying amount is 200-300 mL / m 2 .

[0049] The spraying with the neutral pure water, the control of the pH value, the spraying frequency and the spraying amount provide suitable moisture conditions for the growth of the sprout vegetables, and the improper water is avoided to affect the growth and quality of the sprout vegetables.

[0050] In the application, the culture medium is preferably mixed by coconut husk, perlite and vermiculite at a ratio of 3:1:1.

[0051] In the application, the selected sand millet seeds are specifically full-granular sand millet seeds without diseases and insect pests.

[0052] The application also provides the application of the sand millet sprout vegetable cultivation method rich in gamma-aminobutyric acid in the cultivation of the sand millet sprout vegetables.

[0053] Example 1

[0054] (1) Select high-quality sand millet seeds of the Minqin strain, soak them in clean water for 6 hours, remove the inferior seeds floating on the water surface during the period, then disinfect them with 0.1% sodium hypochlorite solution for 10 minutes, and then rinse them clean.

[0055] (2) Prepare the culture medium, mix coconut husk, perlite and vermiculite at a ratio of 3:1:1, put the disinfected culture medium into the culture tray, then evenly sow the treated seeds on the culture medium, the density is 4 seeds per square centimeter, cover a layer of about 0.5 centimeter thin gauze, and gently compact.

[0056] (3) In the initial stage of cultivation (1-3 days), the environmental temperature is controlled to be 22℃, the humidity is kept at 85%, and the light shielding treatment is performed to promote the seed germination.

[0057] (4) After the seed germination (4-7 days), the temperature is adjusted to be 20℃, the humidity is 75%, and 12 hours of light is provided every day.

[0058] Spray with neutral pure water with pH value of 6.5 every 2 days, the spraying amount is 200 milliliters per square meter, after 7 days, when the seedlings grow to 9 centimeters high, harvest by cutting off the base with scissors.

[0059] Example 2

[0060] (1) Select the Naiman strain of sand millet seeds, soak in clean water for 8 hours, remove the floating seeds, and then sterilize with 0.1% sodium hypochlorite solution for 10 minutes, and then rinse with clean water.

[0061] (2) Use the mixed cultivation medium of coconut husk, perlite and vermiculite in a ratio of 3:1:1, fill the cultivation tray, and spread the treated seeds on the medium at a density of 5 seeds per square centimeter, cover with thin gauze and compact.

[0062] (3) During the initial cultivation period (1-3 days), maintain a temperature of 25°C and a humidity of 90%, and perform shading treatment.

[0063] (4) After germination, control the temperature at 25°C and the humidity at 70%, and provide 12 hours of light per day.

[0064] Spray with neutral pure water with pH value of 7.0 every 2 days, the spraying amount is 300 milliliters per square meter, after 8 days, when the seedlings grow to 10 centimeters high, harvest.

[0065] Example 3

[0066] (1) Select the sand millet seeds of the Ulan Buh product line, soak in clean water for 7 hours, remove the inferior seeds, and then sterilize with 0.1% sodium hypochlorite solution for 10 minutes, and then rinse with clean water.

[0067] (2) The cultivation medium is prepared according to the ratio of coconut husk, perlite and vermiculite 3:1:1, filled into the cultivation tray, and the seed spreading density is 4.5 seeds per square centimeter, covered with thin gauze and compacted.

[0068] (3) During the initial cultivation period, the temperature is 23°C and the humidity is 88%, and shading treatment is performed for 1-3 days.

[0069] (4) After germination, the temperature is 22°C and the humidity is 78%, and 12 hours of light per day.

[0070] Spray with pure water with pH value of 6.8 every 2 days, 250 milliliters per square meter, harvest after 7 days, and the seedlings are 8.5 centimeters high.

[0071] Example 4

[0072] (1) Select high-quality sand millet seeds of the Alxiang product line, soak them in clean water for 8 hours, remove the inferior seeds floating on the water surface during the soaking period, then sterilize with 0.1% sodium hypochlorite solution for 10 minutes, and then rinse with clean water.

[0073] (2) Prepare the culture medium by mixing coconut coir, perlite and vermiculite in a ratio of 3:1:1, sterilize the culture medium, fill the culture tray with the sterilized culture medium, and then evenly spread the treated seeds on the culture medium at a density of 4 seeds per square centimeter, cover with a layer of about 0.5 centimeters of gauze, and gently compact.

[0074] (3) In the early stage of culture (1-3 days), control the environmental temperature at 22°C and maintain the humidity at 85%, and perform shading treatment to promote seed germination.

[0075] (4) After the seeds germinate (4-7 days), adjust the temperature to 20°C and the humidity to 80%, and provide 12 hours of light per day.

[0076] Spray with neutral pure water with a pH value of 6.5 every 2 days at a volume of 200 milliliters per square meter, and after 7 days, harvest when the seedlings grow to a height of 9 centimeters by cutting off the base with scissors.

[0077] Example 5

[0078] (1) Select high-quality sand millet seeds of the Haolaihuore variety, soak them in clean water for 7 hours, remove the poor quality seeds floating on the water during the soaking period, then sterilize the seeds with a 0.1% sodium hypochlorite solution for 10 minutes, and then rinse them clean with clean water.

[0079] (2) Prepare the culture medium by mixing coconut coir, perlite and vermiculite in a ratio of 3:1:1, sterilize the culture medium, fill the culture tray with the sterilized culture medium, and then evenly spread the treated seeds on the culture medium at a density of 5 seeds per square centimeter, cover with a layer of about 0.5 centimeters of gauze, and gently compact.

[0080] (3) In the early stage of culture (1-3 days), control the environmental temperature at 22°C and maintain the humidity at 85%, and perform shading treatment to promote seed germination.

[0081] (4) After the seeds germinate (4-7 days), adjust the temperature to 20°C and the humidity to 75%, and provide 12 hours of light per day.

[0082] Spray with neutral pure water with a pH value of 6.5 every 2 days at a volume of 200 milliliters per square meter, and after 7 days, harvest when the seedlings grow to a height of 9 centimeters by cutting off the base with scissors.

[0083] Test

[0084] The γ-aminobutyric acid (GABA) content in the sprouts obtained in Examples 1-5 was detected, and the specific detection method was as follows:

[0085] The 0.1 g sample of each of the sprouts and seedlings of Examples 1-5 was precisely weighed, ground thoroughly, added to 1 ml of 60% ethanol solution, and oscillated for 2 h at 40 DEG C on a shaking table, centrifuged at 4000 r / min for 10 min, and then 0.5 ml of supernatant was taken, 0.2 ml of 0.2 mmol / L boric acid buffer solution with pH 9.0 was added, 1 ml of 6% phenol solution was added, mixed, and left at room temperature for 5 min. Then, 0.2 ml of NaClO solution with effective chlorine content of 5.6% was added, oscillated thoroughly, boiled in a water bath for 10 min, immediately placed in an ice water bath for 20 min. After the solution appeared blue-green, 0.2 ml of 60% ethanol solution was added, oscillated again, and left. Then, the absorbance value was measured at 645 nm, and the standard curve was y=1.8016x+0.0052 R2=0.9992.

[0086] The detection results are shown in Table 1.

[0087] Table 1 γ-aminobutyric acid (GABA) content (dw / g) in sprouts and seedlings of different local strains of sago

[0088]

[0089] Note: DW: dry weight.

[0090] Based on Table 1, the sprouts and seedlings obtained by the application have high content of γ-aminobutyric acid (GABA).

[0091] It should be finally noted that: the above examples are only used to illustrate the technical solutions of the application but not to limit it, although the application has been described in detail with reference to the above examples, those skilled in the art should understand that: the specific embodiments of the application can be modified or replaced equivalently without departing from the spirit and scope of the application, and any modification or equivalent replacement without departing from the spirit and scope of the application should be covered in the protection scope of the claims of the application.

Claims

1. A method for cultivating Salsop serrata sprouts rich in γ-aminobutyric acid, characterized in that: The following steps are involved: Selecting sago seeds, soaking them with clean water, and then disinfecting them with a sodium hypochlorite solution to obtain treated sago seeds; Sowing the treated sago seeds in a culture medium, covering the surface of the seeds with a layer of gauze after sowing, and carrying out the first stage of cultivation; After the first stage of cultivation is completed, the second stage of cultivation is carried out, and the sprouts are harvested when they grow to a height of 8 to 10 centimeters to obtain the Salsopsis pilosula sprouts rich in gamma-aminobutyric acid.

2. The method for cultivating Salsop serrata sprouts rich in γ-aminobutyric acid according to claim 1, wherein: The soaking time is 6 to 8 hours.

3. The method for cultivating Salsopsis pilosula sprouts rich in γ-aminobutyric acid according to claim 1, wherein: The disinfection specifically comprises the following steps: using 0.1% sodium hypochlorite solution to disinfect the sago seeds for 10 minutes, and then rinsing them with clean water.

4. The method for cultivating Salsopsis pilosula sprouts rich in γ-aminobutyric acid according to claim 1, wherein: The concentration of the sago seeds during sowing is 4 to 5 seeds / cm 2 .

5. The method for cultivating Salsopsis pilosula sprouts rich in γ-aminobutyric acid according to claim 1, characterized in that: The conditions for the first stage of cultivation are: temperature of 20-25°C, humidity of 80%-90%, and shading.

6. The method for cultivating Salsopsis pilosula sprouts rich in γ-aminobutyric acid according to claim 1, characterized in that: The conditions for the second stage of cultivation are: temperature of 15-25°C, humidity of 70%-80%, and sunlight exposure.

7. The method for cultivating Salsopsis pilosula sprouts rich in γ-aminobutyric acid according to claim 1, characterized in that: Neutral purified water was used for spraying in both the first and second stage cultivation, with the pH value controlled at 6.5-7.

0. The spraying frequency was 2 days / time, and the spraying volume was 200-300 mL / m 2 .

8. The method for cultivating γ-aminobutyric acid-rich Salsepium serrata sprouts according to claim 1, characterized in that: The culture medium is prepared by mixing coconut husk, perlite and vermiculite in a ratio of 3:1:

1.

9. The method for cultivating Salsopsis pilosula sprouts rich in γ-aminobutyric acid according to claim 1, characterized in that: The selecting of sago seeds specifically comprises: selecting sago seeds with full grains and no diseases or insect pests.

10. Use of the method for cultivating γ-aminobutyric acid-rich Salsola spinosa sprouts according to any one of claims 1 to 9 in cultivating Salsola spinosa sprouts.

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