Intelligent water-oil balance composition applicable to sensitive skin and application of intelligent water-oil balance composition

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses an intelligent water-oil balance composition suitable for sensitive skin and application of the intelligent water-oil balance composition. Through mutual matching of the components in a proper mass ratio range, a temperature-sensitive receptor of skin can be synergistically regulated and controlled, natural moisturizing generation is promoted, and ultraviolet protection is improved; the intelligent water-oil balance composition provided by the invention can selectively inhibit TRPV1-mediated 5 alpha-reductase activity and ultraviolet-induced oxidative stress at a high temperature in summer, precisely regulate sebum secretion without damaging a barrier, and promote generation of natural moisturizing factors by activating degradation of fibroin in a low-temperature environment in winter; the hydration degree of the cuticle is dynamically adjusted, the technical limitation that a traditional product is used in different seasons and the function of active ingredients is single and one-sided is broken through, cross-seasonal balanced nursing of single-formula self-adaption is achieved, and the skin care product is mild, free of irritation and suitable for sensitive skin.

Description

Technical Field

[0001] The present application relates to the field of cosmetic technology, and in particular to an intelligent water-oil balancing composition suitable for sensitive skin and its application. Background Art

[0002] The root cause of combination skin's "oily summer, dry winter" characteristics lies in an imbalance in the environmental response of sebaceous gland function and barrier homeostasis. High summer temperatures activate epidermal TRPV1 channels and UV-induced 5α-reductase activity, leading to excessive sebum production. Meanwhile, low winter temperatures inhibit ceramide synthase and filaggrin degrading enzymes, reducing the natural moisturizing factor (NMF) and causing dry and flaky skin. Combination-sensitive skin, due to its sensitivity and fragility, overreacts to changes in temperature and humidity. This makes excessive sebum secretion more likely in the summer, even leading to seborrheic dermatitis, while dryness and flaking are more common in the winter, creating a vicious cycle of "oily-dry-sensitive."

[0003] Currently, summer oil-control products rely on astringent ingredients such as salicylic acid and aluminum chloride to control oil by inhibiting the activity of sebaceous gland cells or mechanically blocking pores. However, such ingredients can easily aggravate barrier damage in winter and stimulate TRPV1 to cause tingling in sensitive skin. Winter moisturizing products are mainly composed of occlusive oils and hygroscopic agents. Although they can improve the hydration of the stratum corneum in the short term, they hinder the evaporation of sweat in the high temperature of summer and induce the risk of "acne". In addition, current skin care products lack the ability to dynamically respond to temperature / ultraviolet signals and cannot intelligently switch oil-control / moisturizing modes according to environmental changes. Summary of the Invention

[0004] The present application aims to overcome the shortcomings of the aforementioned prior art and provide an intelligent water-oil balancing composition suitable for sensitive skin and its application. The intelligent water-oil balancing composition of the present application can simultaneously maintain the seasonal balance of sebum secretion and skin hydration, thereby achieving intelligent regulation of "oil control without dryness in summer and moisturizing without acne in winter" while also meeting the gentleness requirements of sensitive skin.

[0005] To achieve the above objectives, the technical solutions adopted in this application are:

[0006] The present application provides an intelligent water-oil balancing composition suitable for sensitive skin, the intelligent water-oil balancing composition comprising an active ingredient derived from medicinal phytomycetes, 4-tert-butyl cyclohexanol, hyaluronic acid, milk thistle seed extract, and sophora flavescens root extract;

[0007] The mass ratio of the medicinal phyllostachys active ingredient, 4-tert-butyl cyclohexanol, hyaluronic acid, milk thistle seed extract and sophora flavescens root extract is (0.5-10): (0.1-5): (0.01-2): (0.01-5): (0.01-2);

[0008] The active ingredient derived from medicinal pore fungus includes at least one of a medicinal pore fungus extract and a medicinal pore fungus-Lactobacillus plantarum bidirectional fermentation product.

[0009] In the technical solution of the present application, the present application matches the components in the above-mentioned mass ratio range with each other to synergistically regulate the skin's temperature-sensitive receptors, promote natural moisturizing production and enhance ultraviolet protection; the intelligent water-oil balance composition provided by the present application can selectively inhibit TRPV1-mediated 5α-reductase activity and ultraviolet-induced oxidative stress under high temperatures in summer, accurately regulate sebum secretion without damaging the barrier, and at the same time promote the production of natural moisturizing factors by activating the degradation of filaggrin in low-temperature environments in winter, dynamically regulate the hydration of the stratum corneum, breaking through the technical limitations of traditional products that are used according to seasons and have a single and one-sided function of active ingredients, and realizing "single formula adaptive" cross-season balanced care, which is gentle and non-irritating and suitable for sensitive skin.

[0010] In the technical solution of the present application, the active ingredients derived from medicinal pore fungi are rich in triterpenoids and polysaccharides, which balance sebum secretion by inhibiting 5α-reductase activity and regulating the miR-29 signaling pathway. At the same time, the polysaccharide components can form a water-retaining film to prevent water loss; 4-tert-butyl cyclohexanol, as an antagonist of the temperature-sensitive receptor TRPV1, can quickly relieve excessive skin oiliness and skin sensitivity caused by high temperatures in summer; hyaluronic acid uses small molecular weight hyaluronic acid, which can penetrate into the granular layer to activate the expression of the filaggrin degrading enzyme Caspase-14, and promote the seasonal adaptive synthesis of natural moisturizing factor (NMF); milk thistle seed extract is rich in silymarin and minerals, which not only provides powerful antioxidant protection for the skin, but also effectively resists the damage of ultraviolet rays in summer, inhibits elastase activity to protect the skin's extracellular matrix; Sophora flavescens root extract contains matrine and flavonoids, has anti-inflammatory and antibacterial effects, can help regulate sebum secretion, and reduce acne. On the basis that the components have the above-mentioned functions, there are still unpredictable synergistic or antagonistic effects between the components. However, based on the component combination and the mass ratio of the components provided in this application, it is possible to ultimately achieve an intelligent water-oil balance composition while maintaining the seasonal balance of sebum secretion and skin moisture content, thereby achieving intelligent regulation of "oil control in summer without drying out, moisturizing in winter without causing acne", while meeting the mildness needs of sensitive skin.

[0011] As a preferred embodiment of the intelligent water-oil balance composition suitable for sensitive skin described in the present application, the mass ratio of the medicinal pore fungus active ingredient, 4-tert-butyl cyclohexanol, hyaluronic acid, milk thistle seed extract and Sophora flavescens root extract is (1-8): (0.2-2): (0.05-0.5): (0.1-1): (0.05-0.5).

[0012] The research in this application found that when the mass ratio of the components in the intelligent water-oil balancing composition is further selected within the above-mentioned range, the mutual coordination effect of the components in the intelligent water-oil balancing composition is better, and the obtained intelligent water-oil balancing composition not only has better oil control effects in summer and moisturizing effects in winter, but also has better UV protection effects; the intelligent water-oil balancing composition provided by this application can selectively inhibit TRPV1-mediated 5α-reductase activity and ultraviolet-induced oxidative stress under high temperatures in summer, accurately regulate sebum secretion without damaging the barrier, and at the same time promote the production of natural moisturizing factors by activating the degradation of filaggrin in the low temperature environment in winter, dynamically regulate the hydration of the stratum corneum, achieve cross-seasonal balanced care, and is suitable for sensitive skin.

[0013] As a preferred embodiment of the intelligent water-oil balance composition suitable for sensitive skin described in the present application, the mass ratio of the medicinal pore fungus active ingredient, 4-tert-butyl cyclohexanol, hyaluronic acid, milk thistle seed extract and Sophora flavescens root extract is (3-5): (0.5-1): (0.2-0.3): (0.3-0.5): (0.1-0.2).

[0014] When the intelligent water-oil balance composition of the present application is preferably within the above-mentioned mass ratio range, it can better achieve the intelligent regulation of "oil control in summer without drying out, moisturizing without acne in winter", and can also improve the product's UV protection effect, meeting the mildness needs of sensitive skin.

[0015] Among them, when the active ingredients of medicinal pore fungus and Sophora flavescens root extract are compounded, the obtained intelligent water-oil balance composition can better intelligently adjust the oil control and moisturizing effects across seasons, and the UV protection effect is also better.

[0016] In some specific embodiments, the active ingredient derived from medicinal pore fungus is a bidirectional fermentation product of medicinal pore fungus and Lactobacillus plantarum.

[0017] The active ingredient derived from medicinal pore fungus used in this application can be well matched with other components. It can accurately regulate sebum secretion in the high temperature of summer without damaging the barrier and enhance ultraviolet protection. At the same time, it can dynamically adjust the hydration of the stratum corneum in the low temperature environment of winter, achieving balanced care across seasons. It is preferred that the active ingredient derived from medicinal pore fungus is the medicinal pore fungus-Lactobacillus plantarum bidirectional fermentation product to achieve better the above-mentioned effects.

[0018] The preparation method of the medicinal phytomycetes-Lactobacillus plantarum bidirectional fermentation product comprises the following steps:

[0019] (1) mixing a Lactobacillus plantarum bacterial liquid and a medicinal pore fungus in a mass ratio of (0.5-3):1 and then performing anaerobically fermentation at a temperature of 37±2°C for 48-72 hours to obtain a fermentation product;

[0020] (2) The fermentation product is cooled to 2-6° C. and then circulated in a microfluidizer at a pressure of 1200-1400 bar and a temperature of 20-30° C. The product is finally crushed and centrifuged, and the supernatant is collected and dried to obtain a medicinal phytomycete-Lactobacillus plantarum bidirectional fermentation product.

[0021] In some specific embodiments, the preparation method of the medicinal porphyromonas extract comprises the following steps:

[0022] The dried fruiting body of medicinal pore fungus was crushed and passed through an 80-mesh sieve, and then added to a 70% ethanol aqueous solution for ultrasonic extraction. The solid-liquid ratio was 1 g:30 mL, the ultrasonic extraction temperature was 40°C, the power was 120 W, and the time was 30 min. After the extraction was completed, the mixture was centrifuged, the supernatant was collected and concentrated and dried to obtain the medicinal pore fungus extract.

[0023] As a preferred embodiment of the intelligent water-oil balancing composition suitable for sensitive skin described in the present application, the preparation method of the Sophora flavescens root extract comprises the following steps:

[0024] The root of Sophora flavescens was dried and crushed, and then added to a deep eutectic solvent for ultrasonic extraction. After the extraction, the mixture was centrifuged, and the supernatant was collected and concentrated to dryness to obtain the Sophora flavescens root extract;

[0025] The deep eutectic solvent includes citric acid and sorbitol.

[0026] The research in this application found that the preparation method of Sophora flavescens root extract, which adopts low eutectic conditions and ultrasonic extraction conditions, can improve the extraction efficiency while also extracting active components that synergize with other components, thereby improving the comprehensive effect of the intelligent water-oil balance composition.

[0027] By adopting the above-mentioned optimized method for preparing Sophora flavescens root extract, the obtained Sophora flavescens root extract and the active ingredient derived from medicinal pore fungus are used in combination, which can selectively inhibit TRPV1-mediated 5α-reductase activity and ultraviolet-induced oxidative stress, accurately regulate sebum secretion under high temperatures in summer, and enhance protection against ultraviolet rays; at the same time, by activating the degradation of filaggrin, the production of natural moisturizing factor is promoted, and the hydration of the stratum corneum is dynamically adjusted under winter conditions, which better breaks through the technical limitations of traditional products that are used according to seasons and have single and one-sided functions of active ingredients, and realizes "single formula adaptive" cross-season balanced care, which is gentle and non-irritating and suitable for sensitive skin.

[0028] As a preferred embodiment of the intelligent water-oil balance composition suitable for sensitive skin described in the present application, the preparation method of the deep eutectic solvent comprises the following steps: mixing citric acid and sorbitol in a molar ratio of 1: (1-2), and then adding ultrapure water to obtain a deep eutectic solvent; the mass percentage of water in the deep eutectic solvent is 15-40%.

[0029] The present application uses the above-mentioned types and specific molar ratios of low eutectic solvents to improve the extraction efficiency of Sophora flavescens root extract, and also enhance the oil control, moisturizing and UV protection effects of Sophora flavescens root extract when applied to the intelligent water-oil balance composition.

[0030] In some specific embodiments, the molar ratio of citric acid to sorbitol is 1:1; and the mass percentage of water in the deep eutectic solvent is 25%.

[0031] As a preferred embodiment of the intelligent water-oil balancing composition suitable for sensitive skin described in the present application, the mass-to-volume ratio of the Sophora flavescens root and the low eutectic solvent is 1g:(20-50)mL, preferably 1g:40mL.

[0032] The Sophora flavescens root and the low eutectic solvent of the present application adopt the above-mentioned mass-to-volume ratio range, and the performance of the obtained Sophora flavescens root extract is better when applied to the intelligent water-oil balance composition.

[0033] As a preferred embodiment of the intelligent water-oil balance composition suitable for sensitive skin described in the present application, the temperature of the ultrasonic extraction is 30-50°C, the power is 100-150W, and the time is 30-90min; preferably, the temperature of the ultrasonic extraction is 40°C, the power is 120W, and the time is 40min.

[0034] The present application adopts the above-mentioned ultrasonic extraction conditions to improve the extraction efficiency of the Sophora flavescens root extract, thereby improving the comprehensive performance of the intelligent water-oil balance composition.

[0035] The present application also provides the use of an intelligent water-oil balancing composition suitable for sensitive skin in the preparation of skin care products.

[0036] The intelligent water-oil balancing composition provided in this application can be used in oil control, moisturizing, and repair products, and further used in sensitive people.

[0037] As a preferred embodiment of the application described in the present application, the skin care product includes at least one of lotion, emulsion, cream, mask, essence, and spray.

[0038] The intelligent water-oil balancing composition provided in the present application can selectively inhibit TRPV1-mediated 5α-reductase activity and ultraviolet-induced oxidative stress, and can also promote the production of natural moisturizing factors by activating the degradation of filaggrin, thereby achieving precise cross-seasonal regulation of sebum secretion and cuticle hydration, and enhancing ultraviolet protection. Therefore, the intelligent water-oil balancing composition provided in the present application can be widely used in the preparation of skin care products.

[0039] The present application also provides a facial cream, which includes the following components in mass percentage: 1-10% intelligent water-oil balance composition, 0.1-1% thickener, 0.5-5% moisturizer, 0.5-5% emulsifier, 0.01-0.3% pH regulator, 0.5-3% preservative, and the balance deionized water.

[0040] In some specific embodiments, the thickener includes at least one of polyacrylate crosspolymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, tara gum, guar gum, ammonium acryloyldimethyltaurate / VP copolymer, and acrylic acid (ester) copolymers.

[0041] In some specific embodiments, the moisturizing agent includes at least one of glycerin, D-panthenol, vitamin B5, 1,3-butylene glycol, 1,2-hexanediol, 1,3-propylene glycol, sodium hyaluronate, tremella polysaccharide, trehalose, betaine, allantoin, sodium hyaluronate, sodium polyacrylate, and hydrogenated lecithin.

[0042] In some specific embodiments, the emulsifier includes at least one of caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraisostearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate.

[0043] In some specific embodiments, the pH adjuster comprises at least one of arginine, disodium EDTA, tromethamine, and disodium EDTA.

[0044] In some specific embodiments, the preservative includes at least one of p-hydroxyacetophenone and polyol.

[0045] Compared with the prior art, this application has the following beneficial effects:

[0046] The present application provides an intelligent water-oil balancing composition suitable for sensitive skin and its application. The present application combines components in an appropriate mass ratio range to synergistically regulate the skin's temperature-sensitive receptors, promote natural moisturizing production and enhance ultraviolet protection; the intelligent water-oil balancing composition provided by the present application can selectively inhibit TRPV1-mediated 5α-reductase activity and ultraviolet-induced oxidative stress under high temperatures in summer, accurately regulate sebum secretion without damaging the barrier, and at the same time promote the production of natural moisturizing factors by activating the degradation of filaggrin in low-temperature winter environments, dynamically regulate the hydration of the stratum corneum, breaking through the technical limitations of traditional products with seasonal use and single and one-sided active ingredient functions, and realizing "single formula adaptive" cross-seasonal balanced care, which is gentle and non-irritating and suitable for sensitive skin. DETAILED DESCRIPTION

[0047] In order to better illustrate the purpose, technical solutions and advantages of this application, this application will be further described below in conjunction with specific embodiments.

[0048] In the following examples and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified, and the components and raw materials used in each parallel experiment are all of the same kind.

[0049] 4-tert-Butylcyclohexanol: purchased from Guangdong Qianjin Chemical Reagent Co., Ltd., product number B2025012011;

[0050] Hyaluronic acid: purchased from Xi'an Yahua Biotechnology Co., Ltd., product number YH22071304;

[0051] Milk thistle seed extract: purchased from Xi'an Shizeyuan Biotechnology Co., Ltd., product number SFJS01;

[0052] Medicinal porphyromonas extract: homemade, the preparation method includes the following steps:

[0053] The dried fruiting body of medicinal pore fungus (purchased from Anhui Boman Pharmaceutical Co., Ltd.) was crushed and passed through an 80-mesh sieve, and then added to a 70% ethanol aqueous solution for ultrasonic extraction. The solid-liquid ratio was 1 g:30 mL, the ultrasonic extraction temperature was 40°C, the power was 120 W, and the time was 30 min. After the extraction was completed, the mixture was centrifuged, the supernatant was collected and concentrated and dried to obtain the medicinal pore fungus extract.

[0054] Medicinal Faculitis spp.-Lactobacillus plantarum bidirectional fermentation product: homemade, the preparation method includes the following steps:

[0055] (1) Lactobacillus plantarum (ATCC BAA-793) was inoculated into MRS liquid medium (purchased from Thermo Fisher Scientific) at an inoculum rate of 3% (V / V), and anaerobically fermented at 37°C for 20 h until the OD600 value reached 1.4 to obtain a Lactobacillus plantarum bacterial solution;

[0056] (2) The dried fruiting bodies of medicinal porphyra (purchased from Anhui Boman Pharmaceutical Co., Ltd.) were crushed to 40 mesh and sterilized at 121°C for 15 minutes to obtain medicinal porphyra powder;

[0057] (3) Mixing medicinal porphyromonas powder and Lactobacillus plantarum liquid in a mass ratio of 1:2, adding 2% g / mL glucose and 0.05% g / mL magnesium sulfate based on the total volume of the medicinal porphyromonas powder and Lactobacillus plantarum liquid, and then anaerobically fermenting at 37°C for 60 hours to obtain a fermentation product;

[0058] (4) The fermentation product was precooled to 4°C and then circulated for 4 times using a microfluidizer at a pressure of 1200 bar, a stirring speed of 600 rpm, and a temperature of 25°C. After the circulatory treatment, the product was crushed and then centrifuged at a speed of 12000 g for 20 min. The supernatant was collected and vacuum-dried at 25°C to obtain the active ingredient 1 derived from medicinal porphyromonas.

[0059] Sophora flavescens root extract 1: Homemade, the preparation method includes the following steps:

[0060] 1) mixing citric acid and sorbitol in a molar ratio of 1:1, and then adding ultrapure water to obtain a deep eutectic solvent; the mass percentage of water in the deep eutectic solvent is 25%;

[0061] 2) The root of Sophora flavescens was dried and crushed, and then added to a deep eutectic solvent for ultrasonic extraction. The mass volume ratio of the Sophora flavescens root and the deep eutectic solvent was 1 g:40 mL. The ultrasonic extraction temperature was 40 ° C., the power was 120 W, and the time was 40 min. After the extraction was completed, centrifugation was completed, and the supernatant was collected and concentrated and dried to obtain the Sophora flavescens root extract.

[0062] Sophora flavescens root extract 2: homemade, the preparation method is different from that of Sophora flavescens root extract 1 in that, in step 1), citric acid and sorbitol are mixed at a molar ratio of 1:4.

[0063] Sophora flavescens root extract 3: homemade. The difference between the preparation method and Sophora flavescens root extract 1 is that in step 1), betaine and glycerol glucoside are mixed in a molar ratio of 1:1, and then ultrapure water is added to obtain a low eutectic solvent.

[0064] Sophora flavescens root extract 4: homemade. The difference between the preparation method and the Sophora flavescens root extract 1 is that the mass volume ratio of the Sophora flavescens root and the deep eutectic solvent in step 2) is 1 g:10 mL.

[0065] Sophora flavescens root extract 5: homemade. The difference between the preparation method and Sophora flavescens root extract 1 is that in step 2), the ultrasonic extraction temperature is 60° C., the power is 160 W, and the time is 40 min.

[0066] Sophora flavescens root extract 6: homemade. The difference between the preparation method and that of Sophora flavescens root extract 1 is that an ethanol aqueous solution with a mass fraction of 75% is used instead of the deep eutectic solvent.

[0067] Examples 1 to 8 and Comparative Examples 1 to 7

[0068] The examples and comparative examples of the present application provide an intelligent water-oil balancing composition suitable for sensitive skin. The components (parts by weight) of the intelligent water-oil balancing composition suitable for sensitive skin are shown in Table 1.

[0069] Table 1

[0070]

[0071]

[0072] The preparation method of the intelligent water-oil balancing composition provided in Example 1 is: mixing the components to obtain the intelligent water-oil balancing composition.

[0073] The preparation methods of the intelligent water-oil balance compositions provided in Examples 2 to 8 and Comparative Examples 1 to 9 are consistent with that in Example 1; if relevant components are not present, they may be omitted.

[0074] Example 9

[0075] The present embodiment provides an intelligent water-oil balancing composition. The only difference between the intelligent water-oil balancing composition and Example 1 is that the Sophora flavescens root extract 2 is used instead of the Sophora flavescens root extract 1.

[0076] Example 10

[0077] The present embodiment provides an intelligent water-oil balancing composition. The only difference between the intelligent water-oil balancing composition and Example 1 is that Sophora flavescens root extract 3 is used instead of Sophora flavescens root extract 1.

[0078] Example 11

[0079] This embodiment of the present application provides an intelligent water-oil balancing composition. The only difference between the intelligent water-oil balancing composition and Example 1 is that Sophora flavescens root extract 4 is used instead of Sophora flavescens root extract 1.

[0080] Example 12

[0081] This embodiment of the present application provides an intelligent water-oil balancing composition. The only difference between the intelligent water-oil balancing composition and Example 1 is that Sophora flavescens root extract 5 is used instead of Sophora flavescens root extract 1.

[0082] Comparative Example 10

[0083] The comparative example of the present application provides an intelligent water-oil balancing composition, the only difference between the intelligent water-oil balancing composition and Example 1 is that the Sophora flavescens root extract 6 is used instead of the Sophora flavescens root extract 1.

[0084] Comparative Example 11

[0085] The comparative example of the present application provides an intelligent water-oil balancing composition. The only difference between the intelligent water-oil balancing composition and Example 1 is that the Lactobacillus / Hypocrea japonica fermentation product extract (purchased from Synsil, Inc., USA) is used instead of the active ingredient derived from medicinal pore fungi.

[0086] Comparative Example 12

[0087] The comparative example of the present application provides an intelligent water-oil balance composition. The only difference between the intelligent water-oil balance composition and Example 1 is that tetrandrine (purchased from Shaanxi Shize Tiancheng Biotechnology Co., Ltd.) is used instead of 4-tert-butyl cyclohexanol.

[0088] Comparative Example 13

[0089] The comparative example of the present application provides an intelligent water-oil balancing composition. The only difference between the intelligent water-oil balancing composition and Example 1 is that methylpropanediol (purchased from Shandong Taixi Chemical Co., Ltd.) is used instead of hyaluronic acid.

[0090] Comparative Example 14

[0091] The comparative example of the present application provides an intelligent water-oil balancing composition. The only difference between the intelligent water-oil balancing composition and Example 1 is that perilla seed extract (purchased from Shaanxi Jianchuang Biotechnology Co., Ltd.) is used instead of milk thistle seed extract.

[0092] Application Examples 1 to 14, Comparative Application Examples 1 to 14, and Blank Application Examples

[0093] The application examples, comparative application examples and blank application examples of the present application provide a facial cream, the components (mass percentage) of which are shown in Table 2; wherein the intelligent water-oil balancing compositions used in Application Examples 1 to 12 are respectively the intelligent water-oil balancing compositions prepared in Examples 1 to 12, for example, the intelligent water-oil balancing composition in Example 1 is used in Application Example 1, the intelligent water-oil balancing composition in Example 2 is used in Application Example 2, and so on; the intelligent water-oil balancing compositions used in Comparative Application Examples 1 to 14 are respectively the intelligent water-oil balancing compositions prepared in Comparative Examples 1 to 14; and the intelligent water-oil balancing compositions used in Application Examples 13 to 14 are the intelligent water-oil balancing compositions prepared in Example 1.

[0094] Table 2

[0095]

[0096] The preparation method of the facial cream provided in Application Example 1 comprises the following steps:

[0097] (1) Mix the humectant and thickener with water, heat to 85±2°C, and homogenize at 1200 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain prefabricated component A;

[0098] (2) Mix the emulsifiers, heat to 85±2°C, and homogenize at 1200 rpm for 4 minutes. After homogenization, keep warm and set aside to obtain prefabricated component B;

[0099] (3) Mix the preservatives and heat to 60±2°C to melt to obtain preformed component C;

[0100] (4) Preparing A component is heated to 80±2℃, and then the Preparing B component is added under the stirring at 250 rpm. After the temperature is decreased to 60±2℃, the Preparing C component is added under the stirring at 250 rpm. Then the temperature is decreased to 40℃, and the smart water-oil balance composition is added under the stirring for 8 min. Finally, the remaining pH regulator is added to adjust the pH to 6.0, and the stirring is stopped. The cream is discharged.

[0101] The preparation method of the cream provided by Examples 2-14, Comparative Examples 1-14, and Blank Example, and Example 1 is consistent, and no related components are added.

[0102] Example 1

[0103] The effect of the smart water-oil balance composition prepared by Examples 1-12 and Comparative Examples 1-14 is explored, including the following aspects:

[0104] 1. In vitro experiments show that the smart water-oil balance composition inhibits 5α-reductase, and the inhibitory ability is higher at high temperature than at low temperature:

[0105] The seasonal difference in skin oil secretion is closely related to the temperature-regulated activity of 5α-reductase. This enzyme can convert testosterone into dihydrotestosterone, which directly stimulates the proliferation of sebaceous glands and lipid synthesis. Previous studies have shown that high temperatures in summer can significantly increase the activity of 5α-reductase, leading to excessive oil secretion on the skin. In this test, the inhibitory ability of the composition on 5α-reductase under different conditions was determined by simulating the temperature in summer (38℃) and winter (16℃). If the inhibitory ability of the composition on 5α-reductase is higher at high temperature in summer than at low temperature in winter, it indicates that the composition has the property of "temperature-sensitive oil control". The test method is as follows:

[0106] (1) Prepare experimental reagents: weigh 1.00 g of the smart water-oil balance composition prepared by the examples and comparative examples, dissolve in phosphate buffer and dilute to 100 mL to obtain a sample working solution with a concentration of 1.0%; weigh 0.42 g of NADPH disodium salt powder, dissolve in phosphate buffer and dilute to 100 mL to obtain a NADPH solution with a concentration of 5 mM; accurately weigh 5.8 mg of testosterone powder, dissolve in 1 mL of DMSO, and then add phosphate buffer and dilute to 100 mL to obtain a testosterone solution with a concentration of 0.2 mM;

[0107] (2) Reaction system: 1 mL of sample working solution, 1 mL of enzyme solution (5α-reductase concentration of 1 mg / mL, from Guangdong University of Chinese Medicine), 1 mL of NADPH solution and 1 mL of testosterone solution were added into the test tube for the sample group; 1 mL of phosphate buffer, 1 mL of enzyme solution, 1 mL of NADPH solution and 1 mL of testosterone solution were added into the test tube for the blank group;

[0108] (3) 5α-reductase inhibition rate detection: the solution in the test tube was shaken gently, 200 μL was taken by a pipette gun and added into a 96-well enzyme-labeled plate, and then was placed into an enzyme-labeled instrument for detection, and the absorbance value at 340 nm was measured as the first detection value A 样品0 (Blank group); after being placed into an enzyme-labeled instrument for detection after incubation at 38°C (simulating summer high temperature) or 16°C (simulating winter low temperature) for 20 minutes, the absorbance value at 340 nm was measured as the second detection value A 酶0 (Blank group); after being placed into an enzyme-labeled instrument for detection after incubation at 38°C (simulating summer high temperature) or 16°C (simulating winter low temperature) for 20 minutes, the absorbance value at 340 nm was measured as the second detection value A 样品20 (Blank group); after being placed into an enzyme-labeled instrument for detection after incubation at 38°C (simulating summer high temperature) or 16°C (simulating winter low temperature) for 20 minutes, the absorbance value at 340 nm was measured as the second detection value A 酶20 (Blank group); each sample was tested in parallel for 3 times to take the average value of the test, and the calculation formula was as follows:

[0109]

[0110] The results are shown in Table 3.

[0111] Table 3

[0112]

[0113]

[0114] As can be seen from Table 3, the inhibition ability of the intelligent water-oil balance composition obtained from Examples 1 to 12 on 5α-reductase is higher in summer high temperature than in winter low temperature, which indicates that the intelligent water-oil balance composition has the "temperature-sensitive oil control" property. Specifically, the inhibition rate of the intelligent water-oil balance composition on 5α-reductase reaches more than 38.4% under high temperature conditions; and the inhibition rate of the intelligent water-oil balance composition on 5α-reductase reaches less than 10.8% under low temperature conditions. The inhibition rate of the intelligent water-oil balance composition obtained from Examples 1 to 7 on 5α-reductase is 38.4% to 84.3% under high temperature conditions, and the inhibition rate of the intelligent water-oil balance composition on 5α-reductase is 5.1% to 10.8% under low temperature conditions, and the high temperature inhibition rate / low temperature inhibition rate is 44 to 16.5, wherein the intelligent water-oil balance composition of Example 1 has the best "temperature-sensitive oil control" effect.

[0115] The inhibition ability of the intelligent water-oil balance composition prepared in Example 1 on 5α-reductase is better than that of Examples 9 to 12, which indicates that the preparation method of the Sophora flavescens root extract has an impact on the oil control performance of the composition.

[0116] And, as can be seen from Comparative Example 1 and Comparative Examples 1-7, when one of the components is not added, or when the other components are added to make up the missing parts, the effects of the present application cannot be achieved; as can be seen from Example 1 and Comparative Examples 8-9, when the mass parts of the components in Comparative Examples 8-9 are not within the range given in the present application, the corresponding effects of the present application cannot be achieved; as can be seen from Example 1 and Comparative Examples 10-14, when other similar components are used instead, the corresponding effects of the present application cannot be achieved.

[0117] 2. Cell experiments demonstrate that the composition has a "seasonal oil control" effect, by regulating the temperature-sensitive receptor TRPV1 and ultraviolet protection.

[0118] The imbalance of seasonal sebum secretion of the skin is closely related to the temperature-sensitive receptor TRPV1 and ultraviolet-induced oxidative stress. High temperature and ultraviolet in summer synergistically activate the TRPV1 channel, triggering calcium ion influx and up-regulating fatty acid synthase (FASN) expression, leading to excessive sebum secretion; low temperature in winter reduces sebum synthesis by inhibiting PPARγ signal. In this experiment, by simulating summer (38℃+UVB) and winter (33℃+dark) environments, the inhibition of the TRPV1 pathway, ultraviolet damage repair, and the bidirectional regulation of seasonal sebum secretion of the composition on SZ95 sebaceous gland cells were verified. The sebum secretion of the composition under the simulated summer conditions is lower than that under the winter conditions, indicating that the composition has a "seasonal oil control" effect.

[0119] The cell line used is human sebaceous gland cell SZ95 (Shanghai Qingqi Biology, BFN60807569), with 7 passages, the summer test conditions are: incubator temperature 38℃, carbon dioxide 5±1%, UVB irradiation (20mJ / cm 2 , wavelength 310nm); the winter test conditions are: incubator temperature 33℃, carbon dioxide 5±1%, dark environment; the cells are cultured according to the grouping, and then tested, the specific tests are TRPV1 expression detection, reactive oxygen species ROS level detection, and neutral lipid content detection, the test methods are as follows:

[0120] (1) The cell suspension was inoculated in a 96-well cell culture plate, with a density of 1.0*10 4 cells per well, 100μL of DMEM medium (Gibco, C11965500BT) was added to each well, and the cells were cultured under summer or winter test conditions for 24h;

[0121] (2) The supernatant was discarded, 100μL of medium was added to the control group, and 100μL of medium containing 0.2% w / v (g / mL) of the intelligent water-oil balance composition prepared in Example and Comparative Example was added to the sample group, respectively;

[0122] (3) After incubation for 48 h under the same conditions, the cells were collected, the supernatant was discarded, and the cells were used for subsequent detection;

[0123] (4) TRPV1 expression detection: The cells were lysed with RIPA lysis buffer containing protease inhibitors (Merck, Shanghai, V900854). The lysed samples were centrifuged at 12000g for 5 minutes. The supernatant was collected and the TRPV1 protein level in the cell supernatant of each group was detected using the human transforming receptor potential cation channel subfamily V member 1 (TRPV1) ELISA kit (Shanghai ELISA Biology, ml106587). The results were calculated using the absorbance OD value. The intracellular TRPV1 protein reduction rate (%) = (1-sample group OD value / control group OD value) * 100%.

[0124] (5) Detection of reactive oxygen species (ROS) levels: A reactive oxygen species (ROS) test kit (Shanghai ELISA, ml092661) was used to detect the oxidative stress level of each group of cells. The specific operation was to resuspend the cell pellet with DCFH-DA probe solution, incubate at 37°C for 1 hour, centrifuge at 1000g for 5 minutes, remove the supernatant to collect the cell pellet, wash twice with PBS, and use a multifunctional microplate reader to detect fluorescence. The excitation wavelength of the detection was 500 nm and the absorption wavelength was 525 nm. The results were calculated using the fluorescence OD value. The reduction rate of intracellular reactive oxygen species (ROS) level (%) = (1-sample group OD value / control group OD value) * 100%.

[0125] (6) Neutral lipid content detection: Nile red dye (Shanghai Yuanye Biotechnology, S02N11G129713) was added to the cells and incubated at 37°C in the dark for 15 min. The cells were washed twice with PBS and fluorescence was detected using a multifunctional microplate reader. The excitation wavelength was 485 nm and the absorption wavelength was 565 nm. The results were calculated using the fluorescence OD value. The intracellular neutral lipid reduction rate (%) = (1-sample group OD value / control group OD value) * 100%;

[0126] The results are shown in Table 4.

[0127] Table 4

[0128]

[0129]

[0130] The results, shown in Table 4, demonstrate the bidirectional effects of the intelligent water-oil balancing composition provided herein on TRPV1 pathway inhibition, UV damage repair, and seasonal sebum secretion regulation in SZ95 sebaceous gland cells. Sebum secretion in simulated summer conditions was lower than in winter, demonstrating its seasonal oil-control effect.

[0131] The TRPV1 protein reduction rate of the intelligent water-oil balance composition prepared in Examples 1-7 is 45.1%-74.7% under summer conditions, the active oxygen ROS level reduction rate is 30.3%-58.0%, and the neutral lipid reduction rate is 26.1%-46.9%; the TRPV1 protein reduction rate of the above intelligent water-oil balance composition is 20.5%-30.4% under winter conditions, the active oxygen ROS level reduction rate is 6.8%-16.3%, and the neutral lipid reduction rate is -22.5%--6.9%.

[0132] The TRPV1 protein reduction rate, active oxygen ROS level reduction rate, and neutral lipid reduction rate of the intelligent water-oil balance composition of Examples 9-12 under summer and winter conditions are all less than that of Example 1, indicating that the preparation method of the Sophora flavescens root extract has an impact on the seasonal oil control performance of the composition.

[0133] Compared with Example 1, when one component is not added or the component is not added while the other component is added to make up the fraction in Comparative Examples 1-7, the TRPV1 pathway inhibition of the prepared intelligent water-oil balance composition on SZ95 sebaceous gland cells, the ultraviolet damage repair, and the bidirectional regulation of seasonal sebum secretion are all less than that of Example 1; as can be seen from Example 1 and Comparative Examples 8-9, when the mass fraction of the component in Comparative Examples 8-9 is not within the range given in the application, the corresponding effects of the application cannot be achieved; as can be seen from Example 1 and Comparative Examples 10-14, when other similar components are used to replace, the corresponding effects of the application cannot be achieved.

[0134] 3. In vitro experiments prove that the composition has a "seasonal moisturizing" effect by promoting the generation of natural moisturizing factors.

[0135] The skin barrier function and moisturizing needs have significant seasonal differences. Low temperature and low humidity in winter can cause disorder of lipid arrangement in the stratum corneum, loss of natural moisturizing factors, and cause dryness and damage to the skin; while high temperature and high humidity in summer can promote the degradation of filaggrin, produce natural moisturizing factors, and increase the water content of the skin. In this experiment, the winter low temperature and low humidity and the summer high temperature and high humidity environment were simulated to verify the effect of the composition on directional enhancement of winter moisturizing capacity in the 3D epidermal model.

[0136] Experimental method: the cell model used is a human 3D epidermal skin model (Guangdong Boxi Biological Technology Co., Ltd.), the summer test conditions are: incubator temperature 38℃, humidity 90±5%, carbon dioxide 5±1%, UVB irradiation (20mJ / cm 2 , wavelength 310nm); the winter test conditions are: incubator temperature 15℃, humidity 50±5%, carbon dioxide 5±1%, dark environment; the 3D epidermal model is cultured and treated according to the grouping, and then tested, and the specific test is skin water content determination, and the test method is as follows:

[0137] (1) 3D epidermal skin models were cultured using EpiGrowth culture medium (Guangdong Boxi Biotechnology Co., Ltd.);

[0138] (2) In the blank control group, 12.5 μL of the blank application example cream was evenly applied on the surface of the epidermal model, and in the sample group, 12.5 μL of the application examples 1-14 and the comparative application examples 1-14 cream were applied, and the cells were cultured in a simulated summer or winter environment for 24 hours;

[0139] (3) Skin moisture content detection: The skin moisture content of the 3D epidermal model was measured using a skin moisture test probe, Corneometer.

[0140] The skin moisturizing ability of the composition is expressed by the rate of increase in skin moisture content, and the calculation formula is as follows:

[0141] Skin moisture content increase rate = (skin moisture content 样品组 / Skin moisture content 空白对照组 -1)*100%

[0142] The results are shown in Table 5.

[0143] Table 5

[0144]

[0145]

[0146] The results, as shown in Table 5, show that the intelligent water-oil balancing compositions prepared in Examples 1-14 increased skin moisture content more effectively in winter than in summer, demonstrating that the intelligent water-oil balancing compositions of the present application promote the production of natural moisturizing factors in low-temperature winter environments by activating filaggrin degradation, thereby dynamically regulating stratum corneum hydration. Specifically, the resulting compositions increased skin moisture content by 9.3% to 11.1% in summer and 59.9% to 85.1% in winter.

[0147] The preparation method of the Sophora flavescens root extract of the intelligent water-oil balancing composition in Examples 9 to 12 has changed, and the effect of the composition on "seasonal moisturizing" is not as good as that in Example 1, indicating that the preparation method of the Sophora flavescens root extract has an impact on the moisturizing performance of the intelligent water-oil balancing composition.

[0148] From the examples 1 and comparative examples 1-7, it can be seen that when one of the components is not added in the composition, or when it is not added while the other component is added to make up the fraction, the effect of the present application cannot be achieved; from the examples 1 and comparative examples 8-9, it can be seen that when the mass fraction of the components in the comparative examples 8-9 is not within the range given in the present application, the corresponding effect of the present application cannot be achieved; from the examples 1 and comparative examples 10-14, it can be seen that when other similar components are used to replace, the corresponding effect of the present application cannot be achieved.

[0149] Effect example 2

[0150] The safety of the face cream prepared by the application example, the comparative application example and the blank application example is explored, which is specifically tested by human skin patch test:

[0151] 30 volunteers, 15 males and 15 females, aged 20-50 years old, are recruited, and the closed patch test method is used. Equal amounts (0.020 mL-0.025 mL) of test samples (face creams prepared by the application example, the comparative application example and the blank application example) are placed in a specific patch tester, which is attached to the arm of the volunteer with a low-sensitization adhesive tape, and is lightly pressed to evenly attach to the skin, and stays for 24 hours. The blank control group is distilled water, and the blank application example is a face cream without the intelligent water-oil balance composition. After 24 hours, the patch tester is removed, and the skin reaction is observed after 0.5 hours, 24 hours and 48 hours, and the results are recorded. The adverse reaction grade of the skin is shown in Table 6 below.

[0152] Table 6

[0153]

[0154] The results show that the face creams prepared by the application example, the comparative application example and the blank application example are all negative reactions after human patch test, and they are safe and non-irritating to human skin.

[0155] Effect example 3

[0156] The application effect of the face cream prepared by the application example, the comparative application example and the blank application example on human body is explored, including the following steps:

[0157] Test 162 Asian adult panelists of 18-60 years old with mixed skin types and self-perceived sensitive skin were randomly divided into 27 groups, 6 persons in each group. The volunteers were tested in summer (from June to August, average temperature above 28°C, average humidity above 80%) or in winter (from December to February, average temperature below 15°C, average humidity below 50%). The test content was to use the sample (the cream prepared by the application examples 1-14, the comparative application example 1-12 and the blank application example) on the whole face once in the morning and once in the evening every day. The data were collected on the 0th day and the 7th day of use. After the volunteers visited, they washed their faces with facial cleanser and sat in the air-conditioned room with temperature of 21±1°C and humidity of 50±10% for 30 minutes.

[0158] The researchers measured the skin moisture content of the cheekbone using the skin moisture test probe Corneometer; measured the trans-epidermal water loss value TEWL of the cheekbone using the skin moisture loss test probe (Tewameter® TM Hex). After the volunteers sat for 2 hours, the researchers collected the oil content data of the forehead using the skin oil test probe (Sebumeter SM 815);

[0159] The oil control effect of the composition was represented by the decrease of skin oil content, the skin moisturizing effect was represented by the improvement of skin moisture content, and the barrier repair ability was represented by the improvement of TEWL value, and the formula was as follows:

[0160] The decrease rate of skin oil content = [(T0sample group-T7sample group) / T0sample group*100%]-[(T0blank application example-T7blank application example) / T0blank application example*100%];

[0161] The improvement rate of skin moisture content = [(T7sample group-T0sample group) / T0sample group*100%]-[(T7blank application example-T0blank application example) / T0blank application example*100%];

[0162] The improvement rate of TEWL value = [(T0sample group-T7sample group) / T0sample group*100%]-[(T0blank application example-T7blank application example) / T0blank application example*100%];

[0163] The results are shown in Table 7.

[0164] Table 7

[0165]

[0166]

[0167]

[0168] ​The results are shown in Table 7. When the technical solution provided in the present application is adopted, the obtained cream has good oil control and moisturizing properties. It can accurately regulate sebum secretion without damaging the barrier under high temperature conditions in summer, and dynamically adjust the hydration of the stratum corneum under low temperature conditions in winter. It breaks through the technical limitations of traditional products that are used according to seasons and have a single active ingredient function, and realizes "single formula adaptive" cross-season balanced care. It is gentle and non-irritating and suitable for sensitive skin.

[0169] Specifically, on the one hand, the facial creams obtained in Application Examples 1 to 14 have a skin oil content improvement rate of 20.1% to 35.2%, a skin moisture content improvement rate of 5.9% to 6.9%, and a TEWL value improvement rate of 13.7% to 23.7% in a summer environment; on the other hand, the facial creams obtained in a winter environment have a skin oil content improvement rate of -5.9% to -2.0%, a skin moisture content improvement rate of 39.0% to 53.6%, and a TEWL value improvement rate of 8.3% to 16.5%.

[0170] The preparation method of the Sophora flavescens root extract of the intelligent water-oil balancing composition in Application Examples 9 to 12 has changed, and the oil control and moisturizing effects of the composition are not as good as those in Application Example 1, indicating that the preparation method of the Sophora flavescens root extract has an impact on the oil control and moisturizing effects of the intelligent water-oil balancing composition.

[0171] It can be seen from Application Example 1 and Comparative Application Examples 1 to 7 that when one of the components is not added to the cream, or when it is not added and the number of other components is supplemented, the effect of the present application cannot be achieved; it can be seen from Application Example 1 and Comparative Application Examples 8 to 9 that when the mass parts of the components in Comparative Application Examples 8 to 9 are not within the range given in the present application, the corresponding effect of the present application cannot be achieved; it can be seen from Application Example 1 and Comparative Application Examples 10 to 14 that when other similar ingredients are used as substitutes, the corresponding effect of the present application cannot be achieved.

[0172] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and are not intended to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present application.

Claims

1. An intelligent water-oil balancing composition suitable for sensitive skin, characterized in that: The intelligent water-oil balance composition comprises an active ingredient derived from medicinal phytomycetes, 4-tert-butyl cyclohexanol, hyaluronic acid, milk thistle seed extract and sophora flavescens root extract; The mass ratio of the medicinal phyllostachys active ingredient, 4-tert-butyl cyclohexanol, hyaluronic acid, milk thistle seed extract and sophora flavescens root extract is (0.5-10): (0.1-5): (0.01-2): (0.01-5): (0.01-2); The active ingredient derived from medicinal pore fungus includes at least one of a medicinal pore fungus extract and a medicinal pore fungus-Lactobacillus plantarum bidirectional fermentation product.

2. The intelligent water-oil balancing composition suitable for sensitive skin according to claim 1, characterized in that: The mass ratio of the medicinal phyllophora active ingredient, 4-tert-butyl cyclohexanol, hyaluronic acid, milk thistle seed extract and sophora flavescens root extract is (1-8): (0.2-2): (0.05-0.5): (0.1-1): (0.05-0.5).

3. The intelligent water-oil balancing composition suitable for sensitive skin according to claim 2, characterized in that: The mass ratio of the medicinal phyllophora active ingredient, 4-tert-butyl cyclohexanol, hyaluronic acid, milk thistle seed extract and sophora flavescens root extract is (3-5): (0.5-1): (0.2-0.3): (0.3-0.5): (0.1-0.2).

4. The intelligent water-oil balancing composition suitable for sensitive skin according to any one of claims 1 to 3, characterized in that: The preparation method of the Sophora flavescens root extract comprises the following steps: The root of Sophora flavescens was dried and crushed, and then added to a deep eutectic solvent for ultrasonic extraction. After the extraction, the mixture was centrifuged, and the supernatant was collected and concentrated to dryness to obtain the Sophora flavescens root extract; The deep eutectic solvent includes citric acid and sorbitol.

5. The intelligent water-oil balancing composition suitable for sensitive skin according to claim 4, characterized in that: The preparation method of the deep eutectic solvent comprises the following steps: mixing citric acid and sorbitol in a molar ratio of 1:(1-2), and then adding ultrapure water to obtain a deep eutectic solvent; the mass percentage of water in the deep eutectic solvent is 15-40%.

6. The intelligent water-oil balancing composition suitable for sensitive skin according to claim 4, characterized in that: The mass volume ratio of the Sophora flavescens root and the deep eutectic solvent is 1g: (20-50)mL.

7. The intelligent water-oil balancing composition suitable for sensitive skin according to claim 4, characterized in that: The ultrasonic extraction is performed at a temperature of 30 to 50° C., a power of 100 to 150 W, and a time of 30 to 90 minutes.

8. Use of the intelligent water-oil balancing composition suitable for sensitive skin according to any one of claims 1 to 7 in the preparation of skin care products.

9. The use according to claim 8, characterized in that The skin care product includes at least one of lotion, emulsion, cream, facial mask, essence and spray.

10. A facial cream, characterized in that The facial cream comprises the following components in percentage by mass: 1-10% of the intelligent water-oil balance composition according to any one of claims 1 to 7, 0.1-1% of a thickener, 0.5-5% of a moisturizer, 0.5-5% of an emulsifier, 0.01-0.3% of a pH regulator, 0.5-3% of a preservative, and the balance deionized water.

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