Microorganism culture device having a function of improving microbial activity

Abstract

The present application belongs to the technical field of microbial culture, and discloses a microbial culture device with the function of improving microbial activity; the device comprises a culture device body and a tank body, and further comprises: a rotor for uniformly distributing oxygen in the tank body, the rotor being rotatably arranged in the tank body; and a multi-element ion sterilization device for releasing multiple active ions, different active ions being capable of inhibiting non-target bacteria and promoting target bacteria to metabolize, the multi-element ion sterilization device being arranged in the rotor. The present application is provided with the multi-element ion sterilization device and a self-feedback ion grid, oxygen is input into the rotor cavity through a T-shaped pipe, is transported to an annular gap through a spiral pipe, and is distributed by centrifugal force when the rotor rotates, gas-liquid exchange membranes realize efficient oxygen dissolution, the multi-element ion sterilization device releases ions to inhibit miscellaneous bacteria and competitive bacteria, and is switched to a nutrient ion release mode to promote target bacteria to metabolize, and the ion grid adsorbs or releases ions through an electrochemical sensor to maintain concentration balance.

Description

Technical Field

[0001] This invention relates to the field of microbial culture technology, and more specifically, to a microbial culture device that enhances the activity of microorganisms. Background Technology

[0002] Microbial culture technology, as a core technology in many fields such as biopharmaceuticals, environmental remediation, and food fermentation, relies on specialized microbial culture devices to operate. Currently, mainstream microbial culture devices mainly consist of key structures such as a tank, an oxygen supply module, a temperature control module, and a stirring module.

[0003] When culturing specific microbial strains, the conventional procedure involves first placing the culture medium and the target microorganism into a tank, then uniformly adjusting the oxygen supply, temperature control, and stirring modules to promote rapid microbial metabolism. However, existing culture devices have significant shortcomings, making it difficult to effectively enhance the activity of the target microbial strain. This is because, during the cultivation process, competing strains and other microorganisms will compete with the target microorganism for nutrients, resulting in lower activity of the target microorganism. Summary of the Invention

[0004] To address the problem that current microbial culture devices in the prior art are unable to improve the activity of target microbial strains, this invention proposes a microbial culture device with the function of improving microbial activity.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a microbial culture device with the function of improving microbial activity, comprising a culture device body and a tank, and further comprising:

[0006] The rotor is used to evenly distribute oxygen inside the tank, and the rotor is installed inside the tank to rotate.

[0007] The multi-element ion sterilizer is used to release a variety of active ions. Different active ions can inhibit non-target bacteria and promote the metabolism of target bacteria. The multi-element ion sterilizer is located inside the rotor.

[0008] The self-feedback ion grid is used to adsorb and release ions in real time via an electrochemical sensor. The self-feedback ion grid is arranged in a ring at the bottom of the inside of the tank. The electrochemical sensor is embedded in the surface of the self-feedback ion grid to monitor the ion concentration at the liquid film interface in real time.

[0009] Furthermore, the top of the inner wall of the tank is provided with a gas-liquid exchange membrane for injecting oxygen into the liquid, the bottom end of the gas-liquid exchange membrane is in contact with the liquid in the tank, and an annular gap is provided between the gas-liquid exchange membrane and the rotor.

[0010] Furthermore, the rotor has a cavity, and the multi-element ion sterilizer is disposed in the cavity. The multi-element ion sterilizer is fixed to the inner wall of the tank by a connecting column. The connecting column is rotatably connected to the rotor. The rotor has two spiral grooves, and a spiral tube is fixed in the spiral groove. The spiral tube is used to connect the cavity and the annular gap. Both the spiral groove and the spiral tube radiate outward from the center of the rotor. A T-shaped tube communicating with the cavity is fixed in the connecting column.

[0011] Furthermore, a fixed arc-shaped plate is fixedly connected to the spiral tube by a fixed block, a movable arc-shaped plate is provided inside the spiral tube, and an elastic plate is provided between the movable arc-shaped plate and the fixed arc-shaped plate. The elastic plate is slidably inserted into the movable arc-shaped plate and the fixed arc-shaped plate. A connecting membrane is fixedly connected between the fixed arc-shaped plate, the movable arc-shaped plate, and the elastic plate and the inner wall of the spiral tube. A movable column that slides through the spiral tube is fixedly connected to the movable arc-shaped plate. A connecting ball is fixedly connected to one end of the movable column. A cylindrical cavity is opened inside the rotor. The connecting ball is slidably connected inside the cylindrical cavity. A spring is fixedly connected between the connecting ball and the cylindrical cavity.

[0012] Furthermore, the tank body includes an outer shell, an inner shell, and an insulation layer. The insulation layer includes multiple interconnected hexagonal frames. A heat-conducting layer is provided at the top and bottom of each hexagonal frame. A filling material is provided inside each hexagonal frame. A thermocouple sensor located within the filling material is provided inside each hexagonal frame. The filling material is a paraffin-based composite material.

[0013] Furthermore, a connecting ring is fixedly connected to the top end of the rotor, the connecting ring is rotatably connected to the inner shell, a ring gear is fixedly connected to the outer wall of the connecting ring, a rotating shaft is rotatably connected to the inner shell, a spherical gear that meshes with the ring gear is fixedly connected to the rotating shaft, and the rotating shaft is connected to an external drive source.

[0014] Furthermore, a rotating column is rotatably connected to the inner shell, and multiple stirring blades are fixedly attached to the rotating column. The stirring blades contain neodymium iron boron particles and are driven by an external annular electromagnetic array.

[0015] The technical effects and advantages of the microbial culture device with the function of improving microbial activity according to the present invention are as follows:

[0016] (1) By setting up a multi-element ion sterilizer and a self-feedback ion grid, oxygen is input into the rotor cavity through a T-shaped tube and transported to the annular gap through a spiral tube. The rotor rotation optimizes the oxygen distribution through centrifugal force, and the gas-liquid exchange membrane achieves efficient oxygen dissolution. The multi-element ion sterilizer releases ions to inhibit miscellaneous bacteria and competing bacteria. It switches to the nutrient ion release mode to promote the metabolism of target bacteria. The ion grid adsorbs or releases ions through an electrochemical sensor to maintain the concentration balance.

[0017] (2) By setting fixed arc plates and movable arc plates, during the rotation of the rotor, the centrifugal force will cause the connecting ball to move away from the center of the rotor. The connecting ball drives the movable arc plate to move through the movable column. The spring is stretched, which increases the distance between the movable arc plate and the fixed arc plate, causing the rotor to rotate faster. The distance between them is larger, and more oxygen and active ions pass through the spiral tube per unit time, so as to achieve the purpose of regulating the amount of oxygen and active ions transported. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0019] Figure 2 This is a cross-sectional schematic diagram of the culture vessel body in this invention;

[0020] Figure 3 This is a schematic cross-sectional view of the tank body in this invention;

[0021] Figure 4 This is a schematic cross-sectional view of the upper end of the tank in this invention;

[0022] Figure 5 This is a schematic cross-sectional view of the rotor in this invention;

[0023] Figure 6 This is a cross-sectional schematic diagram of the rotor and helical tube in this invention;

[0024] Figure 7 For the present invention Figure 3 Enlarged view of point A in the middle;

[0025] Figure 8 This is a schematic diagram of the hexagonal frame structure in this invention;

[0026] Figure 9 This is a cross-sectional view of the hexagonal frame in this invention.

[0027] In the picture:

[0028] 1. Culture vessel body; 2. Tank body; 201. Outer shell; 202. Inner shell; 203. Insulation layer; 3. Rotor; 4. Multi-element ion sterilizer; 5. Gas-liquid exchange membrane; 6. Annular gap; 7. Cavity; 8. Spiral groove; 9. Spiral tube; 10. T-tube; 11. Connecting column; 12. Fixed arc plate; 13. Moving arc plate; 14. Elastic plate; 15. Connecting membrane; 16. Moving column; 17. Connecting ball; 18. Cylindrical cavity; 19. Spring; 20. Connecting ring; 21. Ring gear; 22. Rotating shaft; 23. Circular gear; 24. Rotating column; 25. Stirring plate; 26. Hexagonal frame; 27. Thermal conductive layer; 28. Filling material; 29. ​​Thermocouple sensor; 30. Self-feedback ion grid. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0030] Reference Figures 1-9 A microbial culture device with the function of enhancing microbial activity includes a culture device body 1 and a container 2, and further includes:

[0031] Rotor 3 is used to make the oxygen in tank 2 evenly distributed. Rotor 3 is rotated inside tank 2.

[0032] The multi-electrode ion sterilizer 4 is used to release a variety of active ions. Different active ions can inhibit non-target bacteria and promote the metabolism of target bacteria. The multi-electrode ion sterilizer 4 is installed inside the rotor 3.

[0033] The self-feedback ion grid 30 is used to adsorb and release ions in real time through an electrochemical sensor. The self-feedback ion grid 30 is arranged in a ring at the bottom of the inside of the tank 2. The electrochemical sensor is embedded in the surface of the self-feedback ion grid 30 to monitor the ion concentration at the liquid film interface in real time.

[0034] In use, microorganisms and culture medium are placed in tank 2. Centrifugal force is generated by driving rotor 3 to rotate. Centrifugal force evenly disperses oxygen into tank 2, avoiding local hypoxia or over-oxidation and ensuring uniform oxygen distribution. Multi-element ion sterilizer 4 releases active ions, which can inhibit the growth of competing and miscellaneous bacteria and promote the metabolism of target bacteria, thereby achieving the purpose of inhibiting miscellaneous and competing bacteria and improving the survival rate of target bacteria. Self-feedback ion grid 30 monitors ion concentration in real time through electrochemical sensor and can dynamically adjust grid spacing to adsorb or release ions. Ion concentration can be controlled in real time to assist the survival of target bacteria.

[0035] Reference Figure 3 , Figure 4 and Figure 5 The inner wall of the tank 2 is provided with a gas-liquid exchange membrane 5 for injecting oxygen into the liquid. The bottom end of the gas-liquid exchange membrane 5 is in contact with the liquid in the tank 2. An annular gap 6 is provided between the gas-liquid exchange membrane 5 and the rotor 3. Oxygen enters the annular gap 6, and the gas-liquid exchange membrane 5 dissolves the oxygen into the culture medium. Undissolved oxygen circulates in the upper air layer. The rotor 3 stirs the air to maintain air pressure balance. By setting the gas-liquid exchange membrane 5, the oxygen dissolution efficiency is improved.

[0036] Reference Figure 3 , Figure 4 and Figure 5The rotor 3 has a cavity 7 inside, and the multi-element ion sterilizer 4 is installed in the cavity 7. The multi-element ion sterilizer 4 is fixed to the inner wall of the tank 2 through the connecting column 11. The connecting column 11 is rotatably connected to the rotor 3. The rotor 3 has two spiral grooves 8 inside, and a spiral tube 9 is fixed in the spiral grooves 8. The spiral tube 9 is used to connect the cavity 7 and the annular gap 6. Both the spiral grooves 8 and the spiral tube 9 radiate outward from the center of the rotor 3. A T-shaped tube 10 connected to the cavity 7 is fixed in the connecting column 11. Oxygen is injected into the cavity 7 through the T-shaped tube 10 using an external oxygen input device. The oxygen enters the spiral tube 9. The rotor 3 rotates and generates centrifugal force. Under the action of centrifugal force, the oxygen diffuses along the spiral path into the annular gap 6. The ions released by the multi-element ion sterilizer 4 can enter the tank 2 along with the oxygen.

[0037] Reference Figure 6 A fixed arc-shaped plate 12 is fixedly connected to the spiral tube 9 by a fixed block. A movable arc-shaped plate 13 is provided inside the spiral tube 9. An elastic plate 14 is provided between the movable arc-shaped plate 13 and the fixed arc-shaped plate 12. The elastic plate 14 is slidably inserted into the movable arc-shaped plate 13 and the fixed arc-shaped plate 12. A connecting membrane 15 is fixedly connected between the fixed arc-shaped plate 12, the movable arc-shaped plate 13, and the elastic plate 14 and the inner wall of the spiral tube 9. A movable column 16 that slides through the spiral tube 9 is fixedly connected to the movable arc-shaped plate 13. A connecting ball 17 is fixedly connected to one end of the movable column 16. A cylindrical cavity 18 is opened inside the rotor 3. The connecting ball 17 is slidably connected in the cylindrical cavity 18. A spring 19 is fixedly connected between the connecting ball 17 and the cylindrical cavity 18. When the rotor 3 rotates at a certain speed, in centrifugal... Under the action of force, the connecting ball 17 moves away from the center of the rotor 3, that is, the connecting ball 17 moves towards one end of the cylindrical cavity 18. The connecting ball 17 drives the moving arc plate 13 to move through the moving column 16. The spring 19 is stretched, and the distance between the moving arc plate 13 and the fixed arc plate 12 increases. At this speed, the connecting ball 17 remains in this position, and the distance between the moving arc plate 13 and the fixed arc plate 12 remains at a certain size. Oxygen and ions pass through the moving arc plate 13. When the speed of the rotor 3 increases, the distance between the moving arc plate 13 and the fixed arc plate 12 increases, and the amount of oxygen and ions passing through per unit time increases. The speed of the rotor 3 can be adjusted according to the actual situation, thereby realizing the adjustment of the amount of oxygen input and ion input per unit time.

[0038] Reference Figure 4 , Figure 8 and Figure 9The tank body 2 includes an outer shell 201, an inner shell 202, and an insulation layer 203. The insulation layer 203 includes multiple interconnected hexagonal frames 26. A heat-conducting layer 27 is provided at the top and bottom of each hexagonal frame 26. A filling material 28 is provided inside the hexagonal frame 26. A thermocouple sensor 29 is located inside the filling material 28. The filling material 28 is a paraffin-based composite material. By setting the hexagonal frame 26, the biomimetic honeycomb structure enhances the uniformity of heat conduction. The filling material 28 absorbs or releases heat to buffer temperature fluctuations. The thermocouple sensor 29 monitors the temperature in real time and provides feedback control.

[0039] Reference Figure 7 A connecting ring 20 is fixedly connected to the top of the rotor 3. The connecting ring 20 is rotatably connected to the inner shell 202. A ring gear 21 is fixedly connected to the outer wall of the connecting ring 20. A rotating shaft 22 is rotatably connected to the inner shell 202. A spur gear 23 that meshes with the ring gear 21 is fixedly connected to the rotating shaft 22. The rotating shaft 22 is connected to an external drive source. When it is necessary to drive the rotor 3 to rotate, the external drive source drives the rotating shaft 22 to rotate. The rotating shaft 22 drives the spur gear 23 to rotate. The spur gear 23 drives the connecting ring 20 to rotate through the ring gear 21. The connecting ring 20 drives the rotor 3 to rotate. The connecting column 11 and the multi-element ion sterilizer 4 will not hinder the rotation of the rotor 3.

[0040] Reference Figure 3 A rotating column 24 is rotatably connected to the inner shell 202, and multiple stirring blades 25 are fixed to the rotating column 24. The stirring blades 25 contain neodymium iron boron particles and are driven by an external annular electromagnetic array. The electromagnetic array generates a rotating magnetic field, and the stirring blades 25 rotate with the frequency of the magnetic field to mix the culture medium. Since the stirring blades 25 are made of elastic material, the shear force on the microorganisms is reduced.

[0041] Working principle: When in use, microorganisms and culture medium are placed in tank 2. Centrifugal force is generated by driving rotor 3 to rotate. Centrifugal force evenly disperses oxygen into tank 2, avoiding local hypoxia or over-oxidation. It can make oxygen evenly distributed. Multi-element ion sterilizer 4 releases active ions, which can inhibit the growth of competing bacteria and miscellaneous bacteria, and can also promote the metabolism of target bacteria, thereby achieving the purpose of inhibiting miscellaneous bacteria and competing bacteria and improving the survival rate of target bacteria. The self-feedback ion grid 30 monitors the ion concentration in real time through electrochemical sensor. It can dynamically adjust the grid spacing to adsorb or release ions. The ion concentration can be controlled in real time to help the survival of target bacteria.

[0042] Oxygen enters the annular gap 6, and the gas-liquid exchange membrane 5 dissolves the oxygen into the culture medium. Undissolved oxygen circulates in the upper air layer. The rotor 3 stirs the air to maintain air pressure balance. By setting the gas-liquid exchange membrane 5, the oxygen dissolution efficiency is improved.

[0043] Oxygen is injected into the cavity 7 through the T-tube 10 using an external oxygen input device. The oxygen enters the spiral tube 9, and the rotor 3 rotates to generate centrifugal force. Under the action of centrifugal force, the oxygen diffuses along the spiral path into the annular gap 6. The ions released by the multi-element ion sterilizer 4 can enter the tank 2 along with the oxygen.

[0044] When rotor 3 rotates at a certain speed, under the action of centrifugal force, connecting ball 17 moves away from the center of rotor 3, that is, connecting ball 17 moves towards one end of cylindrical cavity 18. Connecting ball 17 drives moving arc plate 13 through moving column 16, spring 19 is stretched, and the distance between moving arc plate 13 and fixed arc plate 12 increases. At this speed, connecting ball 17 is held in this position, and the distance between moving arc plate 13 and fixed arc plate 12 is kept at a certain size. Oxygen and ions pass through moving arc plate 13. When the speed of rotor 3 increases, the distance between moving arc plate 13 and fixed arc plate 12 increases, and the amount of oxygen and ions passing through per unit time increases. The speed of rotor 3 can be adjusted according to the actual situation, thereby realizing the adjustment of oxygen input and ion input per unit time.

[0045] By setting a hexagonal frame 26, the biomimetic honeycomb structure enhances the uniformity of heat conduction, the filling material 28 absorbs or releases heat to buffer temperature fluctuations, and the thermocouple sensor 29 monitors the temperature in real time and provides feedback control.

[0046] When it is necessary to drive the rotor 3 to rotate, the external drive source drives the rotating shaft 22 to rotate. The rotating shaft 22 drives the spur gear 23 to rotate. The spur gear 23 drives the connecting ring 20 to rotate through the ring gear 21. The connecting ring 20 drives the rotor 3 to rotate. The connecting column 11 and the multi-element ion sterilizer 4 will not hinder the rotation of the rotor 3.

[0047] An electromagnetic array generates a rotating magnetic field, and the stirring plate 25 rotates with the frequency of the magnetic field to mix the culture medium. Since the stirring plate 25 is made of an elastic material, the shear force on the microorganisms is reduced.

[0048] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

[0049] In conclusion, the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A microbial culture device with the function of enhancing microbial activity, comprising a culture device body (1) and a container (2), characterized in that, Also includes: The rotor (3) is used to make the oxygen in the tank (2) evenly distributed. The rotor (3) is rotated inside the tank (2). The multi-electrode ion sterilizer (4) is used to release a variety of active ions. Different active ions can inhibit non-target bacteria and promote the metabolism of target bacteria. The multi-electrode ion sterilizer (4) is installed inside the rotor (3). The self-feedback ion grid (30) is used to adsorb and release ions in real time through an electrochemical sensor. The self-feedback ion grid (30) is arranged in a ring at the bottom of the inside of the tank (2). The electrochemical sensor is embedded in the surface of the self-feedback ion grid (30) to monitor the ion concentration at the liquid film interface in real time. The inner wall of the tank (2) is provided with a gas-liquid exchange membrane (5) for injecting oxygen into the liquid. The bottom end of the gas-liquid exchange membrane (5) is in contact with the liquid in the tank (2). An annular gap (6) is provided between the gas-liquid exchange membrane (5) and the rotor (3). The rotor (3) has a cavity (7) inside, and the multi-element ion sterilizer (4) is installed inside the cavity (7). The multi-element ion sterilizer (4) is fixed to the inner wall of the tank (2) through the connecting column (11). The connecting column (11) is rotatably connected to the rotor (3). The rotor (3) has two spiral grooves (8) inside, and a spiral tube (9) is fixed inside the spiral grooves (8). The spiral tube (9) is used to connect the cavity (7) and the annular gap (6). The spiral grooves (8) and the spiral tube (9) both radiate outward from the center of the rotor (3). A T-shaped tube (10) communicating with the cavity (7) is fixed inside the connecting column (11).

2. The microbial culture device with the function of improving microbial activity according to claim 1, characterized in that, A fixed arc-shaped plate (12) is fixedly connected to the spiral tube (9) by a fixed block. A movable arc-shaped plate (13) is provided inside the spiral tube (9). An elastic plate (14) is provided between the movable arc-shaped plate (13) and the fixed arc-shaped plate (12). The elastic plate (14) is slidably inserted into the movable arc-shaped plate (13) and the fixed arc-shaped plate (12). A connecting membrane (15) is fixedly connected between the fixed arc-shaped plate (12), the movable arc-shaped plate (13) and the elastic plate (14) and the inner wall of the spiral tube (9). A movable column (16) that slides through the spiral tube (9) is fixedly connected to the movable arc-shaped plate (13). A connecting ball (17) is fixedly connected to one end of the movable column (16). A cylindrical cavity (18) is opened inside the rotor (3). The connecting ball (17) is slidably connected in the cylindrical cavity (18). A spring (19) is fixedly connected between the connecting ball (17) and the cylindrical cavity (18).

3. The microbial culture device with the function of improving microbial activity according to claim 2, characterized in that, The tank (2) includes an outer shell (201), an inner shell (202) and a heat insulation layer (203). The heat insulation layer (203) includes a plurality of interconnected hexagonal frames (26). The top and bottom of the hexagonal frames (26) are provided with heat-conducting layers (27). The hexagonal frames (26) are provided with filling material (28). Thermocouple sensors (29) located in the filling material (28) are provided in the hexagonal frames (26). The filling material (28) is a paraffin-based composite material.

4. The microbial culture device with the function of improving microbial activity according to claim 3, characterized in that, A connecting ring (20) is fixedly connected to the top of the rotor (3). The connecting ring (20) is rotatably connected to the inner shell (202). A ring gear (21) is fixedly connected to the outer wall of the connecting ring (20). A rotating shaft (22) is rotatably connected to the inner shell (202). A spherical gear (23) that meshes with the ring gear (21) is fixedly connected to the rotating shaft (22). The rotating shaft (22) is connected to an external drive source.

5. The microbial culture device with the function of improving microbial activity according to claim 4, characterized in that, A rotating column (24) is rotatably connected to the inner shell (202), and a plurality of stirring blades (25) are fixedly connected to the rotating column (24). The stirring blades (25) contain neodymium iron boron particles and are driven by an external annular electromagnetic array.

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