Chinese herbal medicine nursing toothpaste containing hydrolyzed procyanidine as well as preparation method and application of Chinese herbal medicine nursing toothpaste

Abstract

The invention relates to the technical field of toothpaste preparation, in particular to hydrolyzed procyanidine-containing Chinese herbal medicine nursing toothpaste as well as a preparation method and application thereof.The preparation method of the hydrolyzed procyanidine-containing Chinese herbal medicine nursing toothpaste comprises the following preparation steps: adding a humectant, a thickening agent, a surfactant, a sweetening agent and a preservative into deionized water, and uniformly stirring; uniformly stirring to obtain a mixed solution; adding the abrasive into the mixed solution, and uniformly stirring to obtain paste; and adding the active microcapsules into the paste, uniformly stirring, carrying out vacuum degassing, and filling to obtain the Chinese herbal medicine nursing toothpaste containing hydrolyzed procyanidine. The Chinese herbal medicine care toothpaste is fused with hydrolyzed procyanidine, and shows excellent oral care performance. The oral care solution has obvious inhibition capability on a plurality of common oral pathogenic bacteria, has extremely high bacteriostasis rate, and can effectively block bacterial breeding and maintain oral health from the source. Meanwhile, the toothpaste is excellent in deodorization effect and capable of rapidly decomposing oral cavity peculiar smell molecules and remarkably improving oral cavity freshness.

Description

TECHNICAL FIELD

[0001] The present application relates to toothpaste preparation technology, more particularly, it relates to a hydrolyzed procyanidin Chinese herbal medicine care toothpaste and a preparation method and application thereof. BACKGROUND

[0002] Oral health is an important part of human health, and oral diseases such as dental caries, gingivitis, and periodontitis not only affect the normal function of the oral cavity, but also can cause systemic diseases. Toothpaste, as the main product of daily oral care, its efficacy and safety are of great concern. There are many kinds of toothpaste on the market, including ordinary cleaning toothpaste, medicinal toothpaste, and whitening toothpaste. Among them, medicinal toothpaste adds various active ingredients such as fluoride, antibacterial agents, and anti-inflammatory agents to achieve the purpose of preventing and treating oral diseases. However, some chemical components in medicinal toothpaste may cause irritation and damage to the oral mucosa and teeth, and long-term use may also lead to oral flora imbalance. Chinese herbal toothpaste, as a natural and safe oral care product, has been favored by more and more consumers in recent years. Chinese herbal toothpaste usually uses various Chinese herbal medicine extracts with antibacterial, anti-inflammatory, and hemostatic effects as active ingredients, which can effectively prevent and treat oral diseases while reducing the irritation and damage of chemical components to the oral cavity. Procyanidin is a natural polyphenolic compound widely existing in plants, which has strong antioxidant, antibacterial, and anti-inflammatory biological activities. Studies have shown that procyanidin can effectively scavenge free radicals in the oral cavity, inhibit the growth and reproduction of bacteria, and prevent the occurrence of dental caries, gingivitis, periodontitis, and other oral diseases. However, the solubility of procyanidin in water is low, and the bioavailability is not high, which limits its application in oral care products. Based on the above statement, the present application provides a hydrolyzed procyanidin Chinese herbal medicine care toothpaste and a preparation method thereof, which applies hydrolyzed procyanidin to Chinese herbal medicine care toothpaste, thereby improving the antibacterial, anti-inflammatory, and antioxidant effects of the toothpaste and providing more comprehensive protection for oral health. SUMMARY

[0003] In order to solve the technical problems mentioned in the background art, the present application provides a hydrolyzed procyanidin Chinese herbal medicine care toothpaste and a preparation method and application thereof.

[0004] The present application provides a hydrolyzed procyanidin Chinese herbal medicine care toothpaste, which adopts the following technical solution: A hydrolyzed procyanidin Chinese herbal medicine care toothpaste comprises the following raw materials in mass percentage: active microcapsules 8-15%, abrasives 15-25%, humectants 20-35%, thickening agents 0.5-2%, surfactants 1-3%, sweeteners 0.1-0.5%, preservatives 0.1-0.3%, and the balance is deionized water.

[0005] Preferably, the preparation of the active microcapsules comprises the following preparation steps: Step S1, dispersing tara gum and β-cyclodextrin in deionized water at 50-65℃, cooling to room temperature, adding natural product extract and bioactive antibacterial ingredients, adjusting pH to 6.8-7.2, stirring uniformly, obtaining core material solution; Step S2, dispersing xanthan gum and locust bean gum in deionized water, heating to 80-90℃, stirring for 0.5-1h, cooling to 40-45℃, adding low methoxyl pectin and crosslinking agent, adjusting pH to 6.5-7.5, stirring uniformly, obtaining wall material solution; Step S3, adding the core material solution to the wall material solution at a rate of 10-25mL / min, stirring at room temperature for 1-2h, filtering, freeze-drying, obtaining active microcapsules.

[0006] Preferably, the mass ratio of tara gum, β-cyclodextrin, deionized water, natural product extract and bioactive antibacterial ingredients in step S1 is 1-3:3-8:80-100:5-15:1-5.

[0007] Preferably, the natural product extract in step S1 is composed of traditional Chinese medicine extract, hydrolyzed procyanidin and propolis extract with a mass ratio of 10-12:1-3:1-2.

[0008] Preferably, the traditional Chinese medicine extract is one or more of honeysuckle extract, radix scrophulariae extract, cortex phellodendri extract, sophora flavescens extract, glycyrrhiza extract and mint extract.

[0009] Preferably, the bioactive antibacterial ingredients in step S1 include lactoferrin, lysozyme and biological enzymes.

[0010] Preferably, the amount of lactoferrin added in the bioactive antibacterial ingredients is ≥15% of the sum of the mass of biological enzymes and lysozyme.

[0011] Preferably, the biological enzymes are one or more of amylase, protease, glucanase, catalase, peroxidase and lipase.

[0012] Preferably, the enzyme activity of lysozyme is ≥1000U / mg.

[0013] Preferably, the mass ratio of biological enzymes and lysozyme is 1-3:1-1.2.

[0014] Preferably, the mass ratio of xanthan gum, locust bean gum, deionized water, low methoxyl pectin and crosslinking agent in step S2 is 1-3:1-2:90-100:2-5:0.1-0.3.

[0015] Preferably, the mass ratio of the core material solution and the wall material solution in step S3 is 1-1.5:3-6.

[0016] Preferably, the friction agent is one or more of calcium pyrophosphate, light calcium carbonate, calcium hydrogen phosphate dihydrate, and hydrated silica.

[0017] Preferably, the humectant is one or more of glycerin, sorbitol, propylene glycol, and xylitol.

[0018] Preferably, the thickening agent is one or more of sodium carboxymethyl cellulose, hydroxyethyl cellulose, and magnesium aluminum silicate. Preferably, the surfactant is one or more of sodium methyl cocoyl taurate, cocamidopropyl betaine, disodium cocoyl glutamate, sodium lauroyl sarcosinate, and cocamidopropyl-2-hydroxy-3-sulfopropyl betaine.

[0019] Preferably, the sweetener is one or more of sodium saccharin and aspartame.

[0020] Preferably, the preservative is one or more of potassium sorbate and methyl paraben.

[0021] The application also provides a preparation method of a Chinese herbal care toothpaste containing hydrolyzed procyanidins, which adopts the following technical scheme: The preparation method of the Chinese herbal care toothpaste containing hydrolyzed procyanidins comprises the following preparation steps: The humectant, the thickening agent, the surfactant, the sweetener, and the preservative are added to deionized water and stirred uniformly to obtain a mixed solution; the friction agent is added to the mixed solution and stirred uniformly to obtain a paste; the active microcapsules are added to the paste, stirred uniformly, vacuum degassed, and filled to obtain the Chinese herbal care toothpaste containing hydrolyzed procyanidins.

[0022] The Chinese herbal care toothpaste containing hydrolyzed procyanidins prepared by the above preparation method is applied to the prevention and treatment of oral diseases.

[0023] In summary, the application has the following beneficial effects: The application selects hydrolyzed procyanidins as active additives, which have better water solubility than procyanidins, and combines traditional Chinese medicine extracts and propolis extracts to form a natural antibacterial system, which can inhibit the reproduction of oral pathogenic bacteria, effectively block the breeding of bacteria, maintain oral health from the source, and obtain bioactive antibacterial ingredients by compounding lactoferrin, biological enzymes and lysozyme. Lactoferrin can combine with iron ions to inhibit bacterial growth, biological enzymes can decompose food residues in the oral cavity to reduce bacterial substrates, and lysozyme can destroy bacterial cell walls. The three can synergistically enhance the antibacterial effect. The bioactive antibacterial ingredients and natural product extracts synergistically enhance the antioxidant effect of the toothpaste, have excellent deodorizing effect, can quickly decompose oral odor molecules, and significantly improve the oral freshness.

[0024] The application utilizes scientific proportioning of core materials and wall materials, forms a complex embedding system of Ximenia americana gum and β-cyclodextrin, the ring structure of β-cyclodextrin can wrap hydrolyzed procyanidins and other components, reduce the contact with the external environment (such as oxygen and enzymes), and avoid activity degradation; xanthan gum, locust bean gum and low-methoxyl pectin in the wall material form a gel network through Ca²⁺ crosslinking, further protect the core material and realize slow release, avoid inactivation of active ingredients before use, in the process of preparing the wall material solution, first heat xanthan gum and locust bean gum to a high temperature, realize sufficient swelling and dispersion, then form a uniform base colloid solution, then add low-methoxyl pectin and a crosslinking agent, and further crosslink, avoid degradation of low-methoxyl pectin due to glycosidic bond rupture at high temperature, and affect subsequent crosslinking to cause uneven colloid network. DETAILED DESCRIPTION

[0025] The application is further described below in combination with examples.

[0026] The procyanidins (content: 95%) and hydrolyzed procyanidins (content: 95%) used in the examples and comparative examples of the application are all purchased from Fufeng Snoot Biotechnology Co., Ltd.; Ximenia americana gum and xanthan gum are purchased from Jiangsu Jiujia Biotechnology Co., Ltd.; locust bean gum is purchased from Jiangsu Huazhe Biotechnology Co., Ltd.; low-methoxyl pectin is purchased from Jinhua Qianxun Biotechnology Co., Ltd.; propolis extract is purchased from Shandong Guantai Biological Engineering Co., Ltd. Lactoferrin is purchased from Shaanxi Ran'guan Biotechnology Co., Ltd.; lysozyme (enzyme activity: 2000 U / mg) is purchased from Jiangsu Caiwei Biotechnology Co., Ltd.; amylase is purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0027] Examples 1-3 provide a hydrolyzed procyanidin-containing Chinese herbal medicine care toothpaste and a preparation method and application thereof.

[0028] Example 1 The hydrolyzed procyanidin Chinese herbal medicine care toothpaste comprises the following raw materials in percentage by mass: active microcapsules 8%, friction agent 15%, humectant 20%, thickening agent 0.5%, surfactant 1%, sweetener 0.1%, preservative 0.1%, and the balance is deionized water, wherein the friction agent is hydrated silica, the humectant is glycerol, the thickening agent is sodium carboxymethyl cellulose, the surfactant is sodium lauroyl methylamino acid, the sweetener is sodium saccharin, and the preservative is potassium sorbate.

[0029] The preparation of the active microcapsules comprises the following steps: In step S1, tara gum and β-cyclodextrin are dispersed in deionized water at 50 DEG C, and after cooling to room temperature, natural product extract and bioactive antibacterial ingredients are added, the pH is adjusted to 6.8, and stirring is carried out at a stirring speed of 200 rpm for 20 min to obtain a core material solution, wherein the mass ratio of tara gum, β-cyclodextrin, deionized water, natural product extract and bioactive antibacterial ingredients is 1:3:80:5:1, the natural product extract is composed of honeysuckle extract, hydrolyzed procyanidin and propolis extract in a mass ratio of 10:1:1, the bioactive antibacterial ingredients include lactoferrin, amylase and lysozyme, the amount of lactoferrin added is 15% of the sum of the mass of amylase and lysozyme, and the mass ratio of amylase to lysozyme is 1:1. In step S2, xanthan gum and locust bean gum are dispersed in deionized water, heated to 80 DEG C, stirred for 0.5 h, cooled to 40 DEG C, and then low-methoxyl pectin and CaCl2 are added, the pH is adjusted to 6.5, and stirring is carried out uniformly at a stirring speed of 200 rpm to obtain a wall material solution, wherein the mass ratio of xanthan gum, locust bean gum, deionized water, low-methoxyl pectin and CaCl2 is 1:1:90:2:0.1. In step S3, the core material solution is added dropwise to the wall material solution at a rate of 10 mL / min, stirring is carried out at a stirring speed of 100 rpm for 1 h at room temperature, filtration is carried out, and freeze-drying is carried out to obtain active microcapsules, wherein the mass ratio of the core material solution to the wall material solution is 1:3.

[0030] The preparation method of the hydrolyzed procyanidin Chinese herbal medicine care toothpaste comprises the following steps: The humectant, thickening agent, surfactant, sweetener and preservative are added to deionized water, stirring is carried out uniformly at a stirring speed of 400 rpm to obtain a mixed solution, the friction agent is added to the mixed solution, stirring is carried out uniformly at a stirring speed of 200 rpm to obtain a paste, the active microcapsules are added to the paste, stirring is carried out uniformly at a stirring speed of 100 rpm, vacuum degassing is carried out, and filling is carried out to obtain the hydrolyzed procyanidin Chinese herbal medicine care toothpaste.

[0031] Example 2 The hydrolyzed procyanidine Chinese herbal care toothpaste comprises the following raw materials in percentage by mass: active microcapsules 12%, friction agent 20%, humectant 28%, thickening agent 1%, surfactant 2%, sweetener 0.3%, preservative 0.2%, and the balance is deionized water, wherein the friction agent is hydrated silica, the humectant is glycerol, the thickening agent is sodium carboxymethyl cellulose, the surfactant is sodium lauroyl sarcosinate, the sweetener is sodium saccharin, and the preservative is potassium sorbate.

[0032] The preparation of the active microcapsules comprises the following steps: In step S1, the chitosan gum and the beta-cyclodextrin are dispersed in deionized water at 60 DEG C, and after being cooled to room temperature, the natural product extract and the bioactive antibacterial component are added, the pH is adjusted to 7.0, and the stirring speed is 300 rpm for 25 min to obtain a core material solution, wherein the mass ratio of the chitosan gum, the beta-cyclodextrin, the deionized water, the natural product extract and the bioactive antibacterial component is 2:5:90:10:3, the natural product extract is composed of honeysuckle extract, hydrolyzed procyanidine and propolis extract in a mass ratio of 11:2:1.5, the bioactive antibacterial component comprises lactoferrin, amylase and lysozyme, and the amount of lactoferrin added is 18% of the sum of the mass of the amylase and the lysozyme, and the mass ratio of the amylase and the lysozyme is 2:1.1; In step S2, the xanthan gum and the locust bean gum are dispersed in deionized water, heated to 85 DEG C, stirred for 0.8 h, cooled to 42 DEG C, the low methoxyl pectin and CaCl2 are added, the pH is adjusted to 7.0, and the stirring speed is 300 rpm to obtain a wall material solution, wherein the mass ratio of the xanthan gum, the locust bean gum, the deionized water, the low methoxyl pectin and CaCl2 is 2:1.5:95:4:0.2; In step S3, the core material solution is added to the wall material solution at a rate of 15 mL / min, stirred at room temperature at a speed of 200 rpm for 1.5 h, filtered, and freeze-dried to obtain the active microcapsules, wherein the mass ratio of the core material solution and the wall material solution is 1.2:4.5.

[0033] The preparation method of the hydrolyzed procyanidine Chinese herbal care toothpaste comprises the following steps: The humectant, the thickening agent, the surfactant, the sweetener and the preservative are added to the deionized water, stirred uniformly at a speed of 600 rpm to obtain a mixed solution, the friction agent is added to the mixed solution, stirred uniformly at a speed of 300 rpm to obtain a paste, the active microcapsules are added to the paste, stirred uniformly at a speed of 200 rpm, vacuum degassed, and filled to obtain the hydrolyzed procyanidine Chinese herbal care toothpaste.

[0034] Example 3 A hydrolyzed procyanidine Chinese herbal medicine care toothpaste, comprising the following raw materials in mass percentage: active microcapsules 15%, abrasives 25%, humectants 35%, thickening agents 2%, surfactants 3%, sweeteners 0.5%, preservatives 0.3%, and the balance being deionized water, wherein the abrasives are hydrated silica, the humectants are glycerol, the thickening agents are sodium carboxymethyl cellulose, the surfactants are sodium lauroyl methylamino acid, the sweeteners are sodium saccharin, and the preservatives are potassium sorbate.

[0035] The preparation of the active microcapsules comprises the following steps: In step S1, the acacia gum and the beta-cyclodextrin are dispersed in deionized water at 65 DEG C, and after cooling to room temperature, the natural product extract and the bioactive antibacterial component are added, the pH is adjusted to 7.2, and the stirring speed is 400 rpm for 30 min to obtain a core material solution, wherein the mass ratio of the acacia gum, the beta-cyclodextrin, the deionized water, the natural product extract and the bioactive antibacterial component is 3:8:100:15:5, the natural product extract is composed of honeysuckle extract, hydrolyzed procyanidine and propolis extract in a mass ratio of 12:3:2, the bioactive antibacterial component includes lactoferrin, amylase and lysozyme, and the amount of lactoferrin added is 22% of the sum of the mass of amylase and lysozyme, and the mass ratio of amylase to lysozyme is 3:1.2. In step S2, the xanthan gum and the locust bean gum are dispersed in deionized water, heated to 90 DEG C, stirred for 1 h, cooled to 45 DEG C, and then the low-methoxyl pectin and CaCl2 are added, the pH is adjusted to 7.5, and the stirring is uniform, and the stirring speed is 400 rpm to obtain a wall material solution, wherein the mass ratio of the xanthan gum, the locust bean gum, the deionized water, the low-methoxyl pectin and CaCl2 is 3:2:100:5:0.3. In step S3, the core material solution is added to the wall material solution at a rate of 25 mL / min, stirred at room temperature at a speed of 300 rpm for 2 h, filtered, and freeze-dried to obtain the active microcapsules, wherein the mass ratio of the core material solution to the wall material solution is 1.5:6.

[0036] A preparation method of a hydrolyzed procyanidine Chinese herbal medicine care toothpaste, comprising the following steps: The humectants, the thickening agents, the surfactants, the sweeteners and the preservatives are added to the deionized water, and the mixture is stirred uniformly at a speed of 800 rpm to obtain a mixed solution; the abrasives are added to the mixed solution, and the mixture is stirred uniformly at a speed of 400 rpm to obtain a paste; the active microcapsules are added to the paste, and the mixture is stirred uniformly at a speed of 300 rpm, then vacuum degassed, and filled to obtain the hydrolyzed procyanidine Chinese herbal medicine care toothpaste.

[0037] Comparative Example 1 A Chinese herbal medicine care toothpaste containing hydrolyzed procyanidins, comprising the following raw materials in mass percentage: active microcapsules 8%, abrasives 15%, humectants 20%, thickening agents 0.5%, surfactants 1%, sweeteners 0.1%, preservatives 0.1%, and the balance being deionized water, wherein the abrasives are hydrated silica, the humectants are glycerol, the thickening agents are sodium carboxymethyl cellulose, the surfactants are sodium lauroyl methylamino acid, the sweeteners are sodium saccharin, and the preservatives are potassium sorbate.

[0038] The preparation of the active microcapsules comprises the following steps: In step S1, tara gum and β-cyclodextrin are dispersed in deionized water at 50°C, and after cooling to room temperature, natural product extract and bioactive antibacterial ingredients are added, the pH is adjusted to 6.8, and stirring is performed at a stirring speed of 200 rpm for 20 min to obtain a core material solution, wherein the mass ratio of tara gum, β-cyclodextrin, deionized water, natural product extract and bioactive antibacterial ingredients is 1:3:80:5:1, the natural product extract is composed of honeysuckle extract and hydrolyzed procyanidins in a mass ratio of 10:1, the bioactive antibacterial ingredients include lactoferrin, amylase and lysozyme, and the amount of lactoferrin added is 15% of the sum of the mass of amylase and lysozyme, and the mass ratio of amylase and lysozyme is 1:1. In step S2, xanthan gum and locust bean gum are dispersed in deionized water, heated to 80°C, stirred for 0.5 h, cooled to 40°C, and then low-methoxyl pectin and CaCl2 are added, the pH is adjusted to 6.5, and stirring is performed uniformly at a stirring speed of 200 rpm to obtain a wall material solution, wherein the mass ratio of xanthan gum, locust bean gum, deionized water, low-methoxyl pectin and CaCl2 is 1:1:90:2:0.1. In step S3, the core material solution is added dropwise to the wall material solution at a rate of 10 mL / min, stirring is performed at a speed of 100 rpm for 1 h at room temperature, and then filtration, freeze-drying are performed to obtain active microcapsules, wherein the mass ratio of the core material solution and the wall material solution is 1:3.

[0039] A preparation method of a Chinese herbal medicine care toothpaste containing hydrolyzed procyanidins, comprising the following steps: The humectants, thickening agents, surfactants, sweeteners and preservatives are added to deionized water, stirring is performed uniformly at a speed of 400 rpm to obtain a mixed solution, the abrasives are added to the mixed solution, stirring is performed uniformly at a speed of 200 rpm to obtain a paste, the active microcapsules are added to the paste, stirring is performed uniformly at a speed of 100 rpm, vacuum degassing is performed, and then filling is performed to obtain the Chinese herbal medicine care toothpaste containing hydrolyzed procyanidins.

[0040] Comparative Example 2 The proanthocyanidin Chinese herbal medicine care toothpaste comprises the following raw materials in percentage by mass: active microcapsules 8%, friction agent 15%, humectant 20%, thickening agent 0.5%, surfactant 1%, sweetener 0.1%, preservative 0.1%, and the balance is deionized water, wherein the friction agent is hydrated silica, the humectant is glycerol, the thickening agent is sodium carboxymethyl cellulose, the surfactant is sodium lauroyl methylamino acid, the sweetener is sodium saccharin, and the preservative is potassium sorbate.

[0041] The preparation of the active microcapsules comprises the following steps: In step S1, tara gum and β-cyclodextrin are dispersed in deionized water at 50 DEG C, and after cooling to room temperature, natural product extract and bioactive antibacterial ingredients are added, the pH is adjusted to 6.8, and stirring is carried out at a stirring speed of 200 rpm for 20 min to obtain a core material solution, wherein the mass ratio of tara gum, β-cyclodextrin, deionized water, natural product extract and bioactive antibacterial ingredients is 1:3:80:5:1, the natural product extract is composed of honeysuckle extract, proanthocyanidin and propolis extract in a mass ratio of 10:1:1, the bioactive antibacterial ingredients include lactoferrin, amylase and lysozyme, and the amount of lactoferrin added is 15% of the sum of the mass of amylase and lysozyme, and the mass ratio of amylase to lysozyme is 1:1. In step S2, xanthan gum and locust bean gum are dispersed in deionized water, heated to 80 DEG C, stirred for 0.5 h, cooled to 40 DEG C, and then low-methoxyl pectin and CaCl2 are added, the pH is adjusted to 6.5, and stirring is carried out uniformly at a stirring speed of 200 rpm to obtain a wall material solution, wherein the mass ratio of xanthan gum, locust bean gum, deionized water, low-methoxyl pectin and CaCl2 is 1:1:90:2:0.1. In step S3, the core material solution is added dropwise to the wall material solution at a rate of 10 mL / min, stirring is carried out at a stirring speed of 100 rpm for 1 h at room temperature, filtration is carried out, and freeze-drying is carried out to obtain active microcapsules, wherein the mass ratio of the core material solution to the wall material solution is 1:3.

[0042] The preparation method of the proanthocyanidin Chinese herbal medicine care toothpaste comprises the following steps: The humectant, thickening agent, surfactant, sweetener and preservative are added to deionized water, stirring is carried out uniformly at a stirring speed of 400 rpm to obtain a mixed solution, the friction agent is added to the mixed solution, stirring is carried out uniformly at a stirring speed of 200 rpm to obtain a paste, the active microcapsules are added to the paste, stirring is carried out uniformly at a stirring speed of 100 rpm, vacuum degassing is carried out, and filling is carried out to obtain the proanthocyanidin Chinese herbal medicine care toothpaste.

[0043] Comparative Example 3 The hydrolyzed procyanidin Chinese herbal care toothpaste comprises the following raw materials in percentage by mass: active microcapsules 8%, friction agent 15%, humectant 20%, thickening agent 0.5%, surfactant 1%, sweetener 0.1%, preservative 0.1%, and the balance is deionized water, wherein the friction agent is hydrated silica, the humectant is glycerol, the thickening agent is sodium carboxymethyl cellulose, the surfactant is sodium lauroyl methylamino acid, the sweetener is sodium saccharin, and the preservative is potassium sorbate.

[0044] The preparation of the active microcapsules comprises the following steps: In step S1, tara gum and β-cyclodextrin are dispersed in deionized water at 50°C, and after cooling to room temperature, natural product extract and bioactive antibacterial ingredients are added, the pH is adjusted to 6.8, and stirring is performed at a stirring speed of 200 rpm for 20 min to obtain a core material solution, wherein the mass ratio of tara gum, β-cyclodextrin, deionized water, natural product extract and bioactive antibacterial ingredients is 1:3:80:5:1, the natural product extract is composed of honeysuckle extract, hydrolyzed procyanidin and propolis extract in a mass ratio of 10:1:1, the bioactive antibacterial ingredients include lactoferrin, amylase and lysozyme, and the amount of lactoferrin added is 5% of the sum of the mass of amylase and lysozyme, and the mass ratio of amylase to lysozyme is 1:1. In step S2, xanthan gum and locust bean gum are dispersed in deionized water, heated to 80°C, stirred for 0.5 h, cooled to 40°C, and then low-methoxyl pectin and CaCl2 are added, the pH is adjusted to 6.5, and stirring is performed uniformly at a stirring speed of 200 rpm to obtain a wall material solution, wherein the mass ratio of xanthan gum, locust bean gum, deionized water, low-methoxyl pectin and CaCl2 is 1:1:90:2:0.1. In step S3, the core material solution is added dropwise to the wall material solution at a rate of 10 mL / min, stirring is performed at a speed of 100 rpm for 1 h at room temperature, and then filtration and freeze-drying are performed to obtain active microcapsules, wherein the mass ratio of the core material solution to the wall material solution is 1:3.

[0045] The preparation method of the hydrolyzed procyanidin Chinese herbal care toothpaste comprises the following steps: The humectant, thickening agent, surfactant, sweetener and preservative are added to deionized water, stirring is performed uniformly at a speed of 400 rpm to obtain a mixed solution, the friction agent is added to the mixed solution, stirring is performed uniformly at a speed of 200 rpm to obtain a paste, the active microcapsules are added to the paste, stirring is performed uniformly at a speed of 100 rpm, vacuum degassing is performed, and then filling is performed to obtain the hydrolyzed procyanidin Chinese herbal care toothpaste.

[0046] Comparative Example 4 The hydrolyzed procyanidin Chinese herbal care toothpaste comprises the following raw materials in percentage by mass: active microcapsules 8%, friction agent 15%, humectant 20%, thickening agent 0.5%, surfactant 1%, sweetener 0.1%, preservative 0.1%, and the balance is deionized water, wherein the friction agent is hydrated silica, the humectant is glycerol, the thickening agent is sodium carboxymethyl cellulose, the surfactant is sodium lauroyl methylamino acid, the sweetener is sodium saccharin, and the preservative is potassium sorbate.

[0047] The preparation of the active microcapsules comprises the following steps: In step S1, the acacia gum and β-cyclodextrin are dispersed in deionized water at 50 DEG C, and after cooling to room temperature, the natural product extract and the bioactive antibacterial component are added, the pH is adjusted to 6.8, and the stirring speed is 200 rpm for 20 min to obtain the core material solution, wherein the mass ratio of the acacia gum, β-cyclodextrin, deionized water, natural product extract and bioactive antibacterial component is 1:3:80:5:1, the natural product extract is composed of honeysuckle extract, hydrolyzed procyanidin and propolis extract in a mass ratio of 10:1:1, the bioactive antibacterial component includes lactoferrin and amylase, and the amount of lactoferrin added is 15% of the mass of amylase; In step S2, the xanthan gum and locust bean gum are dispersed in deionized water, heated to 80 DEG C, stirred for 0.5 h, cooled to 40 DEG C, and then the low methoxyl pectin and CaCl2 are added, the pH is adjusted to 6.5, and the stirring speed is 200 rpm to obtain the wall material solution, wherein the mass ratio of the xanthan gum, locust bean gum, deionized water, low methoxyl pectin and CaCl2 is 1:1:90:2:0.1. In step S3, the core material solution is added to the wall material solution at a rate of 10 mL / min, stirred at room temperature at a speed of 100 rpm for 1 h, filtered, and freeze-dried to obtain the active microcapsules, wherein the mass ratio of the core material solution to the wall material solution is 1:3.

[0048] The preparation method of the hydrolyzed procyanidin Chinese herbal care toothpaste comprises the following steps: The humectant, thickening agent, surfactant, sweetener and preservative are added to the deionized water, stirred uniformly at a speed of 400 rpm to obtain a mixed solution, the friction agent is added to the mixed solution, stirred uniformly at a speed of 200 rpm to obtain a paste, the active microcapsules are added to the paste, stirred uniformly at a speed of 100 rpm, vacuum degassed, and filled to obtain the hydrolyzed procyanidin Chinese herbal care toothpaste.

[0049] Comparative Example 5 The hydrolyzed procyanidin Chinese herbal care toothpaste comprises the following raw materials in percentage by mass: active microcapsules 8%, friction agent 15%, humectant 20%, thickening agent 0.5%, surfactant 1%, sweetener 0.1%, preservative 0.1%, and the balance is deionized water, wherein the friction agent is hydrated silica, the humectant is glycerol, the thickening agent is sodium carboxymethyl cellulose, the surfactant is sodium lauroyl methylamino acid, the sweetener is sodium saccharin, and the preservative is potassium sorbate.

[0050] The preparation of the active microcapsules comprises the following steps: In step S1, the acacia gum and β-cyclodextrin are dispersed in deionized water at 50 DEG C, and after cooling to room temperature, the natural product extract and the bioactive antibacterial component are added, the pH is adjusted to 6.8, and the stirring speed is 200 rpm for 20 min to obtain the core material solution, wherein the mass ratio of the acacia gum, β-cyclodextrin, deionized water, natural product extract and bioactive antibacterial component is 1:3:80:5:1, the natural product extract is composed of honeysuckle extract, hydrolyzed procyanidin and propolis extract in a mass ratio of 10:1:1, the bioactive antibacterial component includes lactoferrin and lysozyme, and the amount of lactoferrin added is 15% of the mass of lysozyme. In step S2, the xanthan gum and locust bean gum are dispersed in deionized water, heated to 80 DEG C, stirred for 0.5 h, cooled to 40 DEG C, and then the low methoxyl pectin and CaCl2 are added, the pH is adjusted to 6.5, and the stirring speed is 200 rpm to obtain the wall material solution, wherein the mass ratio of the xanthan gum, locust bean gum, deionized water, low methoxyl pectin and CaCl2 is 1:1:90:2:0.1. In step S3, the core material solution is added to the wall material solution at a rate of 10 mL / min, stirred at room temperature at a speed of 100 rpm for 1 h, filtered, and freeze-dried to obtain the active microcapsules, wherein the mass ratio of the core material solution and the wall material solution is 1:3.

[0051] The preparation method of the hydrolyzed procyanidin Chinese herbal care toothpaste comprises the following steps: The humectant, thickening agent, surfactant, sweetener and preservative are added to the deionized water, stirred uniformly at a speed of 400 rpm to obtain a mixed solution, the friction agent is added to the mixed solution, stirred uniformly at a speed of 200 rpm to obtain a paste, the active microcapsules are added to the paste, stirred uniformly at a speed of 100 rpm, vacuum degassed, and then filled to obtain the hydrolyzed procyanidin Chinese herbal care toothpaste.

[0052] Comparative Example 6 A Chinese herbal medicine care toothpaste containing hydrolyzed procyanidins, comprising the following raw materials in mass percentage: active microcapsules 8%, abrasives 15%, humectants 20%, thickening agents 0.5%, surfactants 1%, sweeteners 0.1%, preservatives 0.1%, and the balance being deionized water, wherein the abrasives are hydrated silica, the humectants are glycerol, the thickening agents are sodium carboxymethyl cellulose, the surfactants are sodium lauroyl methylamino acid, the sweeteners are sodium saccharin, and the preservatives are potassium sorbate.

[0053] The preparation of the active microcapsules comprises the following steps: In step S1, tara gum and β-cyclodextrin are dispersed in deionized water at 50°C, and after cooling to room temperature, natural product extract and bioactive antibacterial ingredients are added, the pH is adjusted to 6.8, and stirring is performed at a stirring speed of 200 rpm for 20 min to obtain a core material solution, wherein the mass ratio of tara gum, β-cyclodextrin, deionized water, natural product extract and bioactive antibacterial ingredients is 1:3:80:5:1, the natural product extract is composed of honeysuckle extract, hydrolyzed procyanidins and propolis extract in a mass ratio of 10:1:1, the bioactive antibacterial ingredients include lactoferrin, amylase and lysozyme, and the amount of lactoferrin added is 15% of the sum of the mass of amylase and lysozyme, and the mass ratio of amylase to lysozyme is 1:1. In step S2, xanthan gum, locust bean gum, low-methoxyl pectin and CaCl2 are dispersed in deionized water, heated to 80°C, stirred for 0.5 h, cooled to 40°C, the pH is adjusted to 6.5, and stirring is performed uniformly at a stirring speed of 200 rpm to obtain a wall material solution, wherein the mass ratio of xanthan gum, locust bean gum, deionized water, low-methoxyl pectin and CaCl2 is 1:1:90:2:0.1. In step S3, the core material solution is added dropwise to the wall material solution at a rate of 10 mL / min, stirring is performed at a speed of 100 rpm at room temperature for 1 h, filtration is performed, and freeze-drying is performed to obtain active microcapsules, wherein the mass ratio of the core material solution to the wall material solution is 1:3.

[0054] A preparation method of a Chinese herbal medicine care toothpaste containing hydrolyzed procyanidins, comprising the following steps: The humectants, thickening agents, surfactants, sweeteners and preservatives are added to deionized water, stirring is performed uniformly at a speed of 400 rpm to obtain a mixed solution, the abrasives are added to the mixed solution, stirring is performed uniformly at a speed of 200 rpm to obtain a paste, the active microcapsules are added to the paste, stirring is performed uniformly at a speed of 100 rpm, vacuum degassing is performed, and filling is performed to obtain the Chinese herbal medicine care toothpaste containing hydrolyzed procyanidins.

[0055] Performance test The Chinese herbal medicine care toothpaste containing hydrolyzed procyanidins prepared in Examples 1-3 and Comparative Examples 1-6 was subjected to antibacterial and deodorization performance tests: Antibacterial rate: The antibacterial performance test was carried out according to the industry standard WS / T 650-2019 "Evaluation method for antibacterial and bacteriostatic effect", the test strains were Staphylococcus aureus, Escherichia coli and Candida albicans, and the carrier immersion quantitative bacteriostatic test was used for detection; Deodorization rate: 54 subjects aged 18-65 years were randomly divided into 9 groups, the subjects were not limited to men and women, and the teeth and gums were in good health, without oral mucosa diseases, without systemic diseases such as digestive system, without periodontal treatment in the past 3 weeks, without the habit of drinking and smoking, women were not in the menstrual period, and the breath value was above 130; Measurement of baseline breath value T1: the subjects did not brush their teeth in the morning, and the breath value was detected by hydrogen sulfide analyzer on an empty stomach, 3 times were measured, and the average value was taken as T1; Measurement of breath value T2 after use: the subjects used the toothpaste prepared in Examples 1-3 and Comparative Examples 1-6 for brushing, the amount of toothpaste was 1.5 g, and the brushing was completed within 3 min, and the breath value was measured immediately after brushing, 3 times were measured, and the average value was taken as T2, Deodorization rate: T = (T1-T2) / T1x100%; The specific test results are shown in Table 1.

[0056] Table 1 Performance parameters of Chinese herbal medicine care toothpaste prepared in Examples 1-3 and Comparative Examples 1-6 As shown in Table 1, the Chinese herbal medicine care toothpaste prepared in the application combines the unique efficacy of hydrolyzed procyanidins, and exhibits excellent oral care performance. It has a significant inhibitory effect on a variety of common oral pathogenic bacteria, with a very high bacteriostatic rate, which can effectively block the growth of bacteria and maintain oral health from the source. At the same time, the deodorization effect of the toothpaste is excellent, which can quickly decompose oral odor molecules and significantly improve the oral freshness, bringing a lasting fresh experience to the user.

[0057] The specific embodiments are merely an explanation of the application, and are not a limitation of the application. Those skilled in the art can make modifications to the embodiments without creative contribution after reading the specification, as long as the modifications are within the scope of the claims of the application.

Claims

1. A Chinese herbal medicine care toothpaste containing hydrolyzed proanthocyanidins, characterized in that: The invention comprises the following raw materials in percentage by mass: 8-15% of active microcapsules, 15-25% of abrasives, 20-35% of moisturizers, 0.5-2% of thickeners, 1-3% of surfactants, 0.1-0.5% of sweeteners, 0.1-0.3% of preservatives, and the balance being deionized water.

2. The Chinese herbal medicine care toothpaste containing hydrolyzed proanthocyanidins according to claim 1, characterized in that: The preparation of the active microcapsules includes the following preparation steps: Step S1, dispersing tsugae gum and β-cyclodextrin in deionized water at 50-65° C., cooling to room temperature, adding natural product extract and bioactive antibacterial component, adjusting the pH to 6.8-7.2, and stirring uniformly to obtain a core material solution; Step S2, dispersing xanthan gum and locust bean gum in deionized water, heating to 80-90° C., stirring for 0.5-1 h, cooling to 40-45° C., adding low-methoxy pectin and a cross-linking agent, adjusting the pH to 6.5-7.5, and stirring evenly to obtain a wall material solution; Step S3: adding the core material solution dropwise to the wall material solution at a rate of 10-25 mL / min, stirring and reacting at room temperature for 1-2 hours, filtering, and freeze-drying to obtain active microcapsules.

3. The Chinese herbal medicine care toothpaste containing hydrolyzed proanthocyanidins according to claim 2, characterized in that: The natural product extract in step S1 is composed of a traditional Chinese medicine extract, hydrolyzed proanthocyanidins, and propolis extract in a mass ratio of 10-12:1-3:1-2.

4. The Chinese herbal medicine care toothpaste containing hydrolyzed proanthocyanidins according to claim 3, characterized in that: The Chinese medicine extract is one or more of honeysuckle extract, scutellaria baicalensis extract, phellodendron amurense extract, sophora flavescens extract, liquorice extract and mint extract.

5. The Chinese herbal medicine care toothpaste containing hydrolyzed proanthocyanidins according to claim 2, characterized in that: The bioactive antibacterial components in step S1 include lactoferrin, lysozyme and biological enzymes; the amount of lactoferrin added to the bioactive antibacterial components must be ≥ 15% of the sum of the mass of the biological enzymes and lysozyme.

6. The Chinese herbal medicine care toothpaste containing hydrolyzed proanthocyanidins according to claim 5, characterized in that: The biological enzyme is one or more of amylase, protease, glucanase, catalase, peroxidase and lipase; the enzyme activity of the lysozyme is ≥1000 U / mg; and the mass ratio of the biological enzyme to the lysozyme is 1-3:1-1.

2.

7. The Chinese herbal medicine care toothpaste containing hydrolyzed proanthocyanidins according to claim 2, characterized in that: In step S1, the mass ratio of the natural product extract to the bioactive antibacterial component is 5-15:1-5.

8. The Chinese herbal medicine care toothpaste containing hydrolyzed proanthocyanidins according to claim 1, characterized in that: The surfactant is one or more of sodium methyl cocoyl taurate, cocamidopropyl betaine, disodium cocoyl glutamate, sodium lauroyl sarcosinate, and cocamidopropyl-2-hydroxy-3-sulfopropyl betaine.

9. A method for preparing a Chinese herbal medicine toothpaste containing hydrolyzed proanthocyanidins according to any one of claims 1 to 8, characterized in that: The method comprises the following preparation steps: A moisturizer, a thickener, a surfactant, a sweetener and a preservative are added to deionized water and stirred evenly to obtain a mixed liquid; an abrasive is added to the mixed liquid and stirred evenly to obtain a paste; active microcapsules are added to the paste, stirred evenly, vacuum degassed, and filled to obtain a Chinese herbal medicine care toothpaste containing hydrolyzed proanthocyanidins.

10. Use of the Chinese herbal medicine toothpaste containing hydrolyzed proanthocyanidins according to any one of claims 1 to 8 in preventing and treating oral diseases.

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