Anammox screening enrichments and methods of use thereof

Abstract

The application discloses an anaerobic ammonia oxidation screening enriching device and a use method thereof, and relates to the technical field of sewage treatment. The device comprises a reaction tank, an enrichment collecting bag and a hoisting mechanism. The bottom of the reaction tank is provided with a water inlet and a sludge discharge port, and the upper part is provided with a water outlet. The reaction tank is used for containing sewage. The enrichment collecting bag is immersed in the sewage. A plurality of enrichment collecting bags are hung in the reaction tank. The enrichment collecting bag can be water-permeable and air-permeable, and the inside of the enrichment collecting bag contains a filler for culturing anaerobic ammonia oxidation bacteria. The hoisting mechanism is used for hoisting the enrichment collecting bag for viewing or hoisting the enrichment collecting bag in and out of the reaction tank. Alternatively, a plurality of enrichment collecting bags are hung on the hoisting mechanism, and the enrichment collecting bags are located in the reaction tank. The anaerobic ammonia oxidation enriching and culturing device and the use method thereof can realize the enrichment and screening of anaerobic ammonia oxidation bacteria quickly, and can realize the recycling of anaerobic ammonia oxidation bacteria.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of sewage treatment, in particular to an anaerobic ammonia oxidation screening enriching device and a use method thereof. BACKGROUND

[0002] The large emission of nitrogen elements can cause the pollution of natural water bodies, and the eutrophication of water bodies can lead to water pollution and the destruction of aquatic ecosystems. Therefore, it is extremely important to control the pollution of water bodies.

[0003] Anaerobic ammonia oxidation bacteria use NH4 + -N as the electron donor, NO2 - -N as the electron acceptor and generate N2 and a small amount of NO3 - -N biological reaction. Compared with the traditional nitrification and denitrification process, anaerobic ammonia oxidation denitrification has the advantages of no need for dissolved oxygen and external carbon source, low sludge yield, small aeration quantity, and small land occupation, etc., and is a new type of denitrification technology with economy, high efficiency, green and low carbon, which has become a research hotspot in the field of water treatment. However, in the actual enrichment and cultivation process, the problem of "running bacteria" exists.

[0004] The current anaerobic ammonia oxidation bacteria use the method of setting fillers to intercept bacteria, or use a three-phase separator to separate anaerobic ammonia oxidation bacteria. However, due to the small amount of sludge produced by anaerobic ammonia oxidation bacteria, and the nitrogen gas produced in the anaerobic ammonia oxidation reaction process, the bacteria exist in the water body in a free state. In actual use, the interception effect of anaerobic ammonia oxidation bacteria is not ideal, and the problem of running bacteria is still serious, which leads to low efficiency of anaerobic ammonia oxidation bacteria enrichment and cultivation, and the enriched anaerobic ammonia oxidation bacteria cannot be transferred for inoculation, and are only suitable for in-situ application. Because the anaerobic ammonia oxidation bacteria grow and proliferate slowly, the intergenerational cycle is long, and the in-situ application limits the use of anaerobic ammonia oxidation technology. Therefore, it is of great significance to study how to collect the enriched anaerobic ammonia oxidation bacteria, provide a prerequisite for the ex-situ application of anaerobic ammonia oxidation process, and promote the carbon reduction goal of sewage treatment with high nitrogen content in China. SUMMARY

[0005] The purpose of the present application is to provide an anaerobic ammonia oxidation screening enriching device and a use method thereof, so as to solve the problems existing in the prior art, and to realize the rapid enrichment and screening of anaerobic ammonia oxidation bacteria, and the recycling of anaerobic ammonia oxidation bacteria.

[0006] To achieve the above purpose, the present application provides the following solutions:

[0007] The application provides an anaerobic ammonia oxidation screening enriching device, which comprises a reaction tank, an enriching collecting bag and a lifting mechanism, the bottom of the reaction tank is provided with a water inlet and a sludge discharge port, and the upper part is provided with a water outlet, the reaction tank is used for containing sewage, the enriching collecting bag is immersed in the sewage, a plurality of enriching collecting bags are hung in the reaction tank, the enriching collecting bag can be water-permeable and air-permeable, and the inside of the enriching collecting bag contains a filler for culturing anaerobic ammonia oxidation bacteria, and the lifting mechanism is used for lifting the enriching collecting bag for viewing or lifting in and out of the reaction tank.

[0008] Preferably, a support plate is detachably arranged in the reaction tank, a plurality of filter holes are uniformly distributed on the support plate, and the enriching collecting bag is placed on the support plate; the diameter of the filter hole is 5mm-10mm; a bearing ring or a plurality of bearing blocks are arranged on the inner wall of the reaction tank, and the support plate is lapped on the bearing ring or the bearing blocks; the support plate is located at 1 / 3-1 / 2 of the height of the reaction tank; a connecting hole connected with a lifting chain is arranged on the support plate, and the lifting chain is connected with the lifting mechanism.

[0009] Preferably, the enriching collecting bag comprises a plurality of sealable bags with a volume of 0.1m³-1m³; the material of the enriching collecting bag comprises 600g of non-woven fabric, a drawstring channel is arranged on the bag opening of each enriching collecting bag, the drawstring channel is double-layer non-woven fabric, a pair of drawstrings are reversely and symmetrically arranged in the drawstring channel, an adjusting lock is arranged on the outer end of each drawstring, and the ends of the two drawstrings are used for hanging on the reaction tank or the lifting mechanism.

[0010] Preferably, the enriching collecting bag comprises one sample bag with a volume of 0.1m³ and a plurality of culture bags with a volume greater than 0.1m³.

[0011] Preferably, the filler comprises iron-copper modified biochar, diatomite and nanocellulose, and the ratio of iron-copper modified biochar: diatomite: nanocellulose is (0.8-1):(0.5-1):(0.2-1).

[0012] Preferably, a cover is arranged on the reaction tank, a manhole is arranged on the cover, the size of the manhole is 80cm*80cm, a ladder is arranged on the side wall of the reaction tank, the height of the ladder is at least the height of the reaction tank, and a hook is arranged on the top of the inner wall of the reaction tank, and the hook is used for hanging the enriching collecting bag.

[0013] Preferably, a cofferdam is arranged around the bottom of the reaction tank along the outer wall of the reaction tank, the sludge discharge port is located in the cofferdam, an outer discharge port is arranged on the side wall of the cofferdam, and valves are arranged on the water inlet port, the water outlet port, the sludge discharge port and the outer discharge port.

[0014] Preferably, the material of the reaction tank comprises stainless steel or carbon steel after corrosion treatment, the wall thickness of the reaction tank is 3-10 cm, and the hoisting mechanism is an electric hoist which is welded to the inner wall of the top of the reaction tank.

[0015] The application also relates to a use method of the anaerobic ammonia oxidation screening enriching device.

[0016] S1, first, water test is conducted on the reaction tank, and after the reaction tank is detected to be watertight, the water is discharged;

[0017] S2, the incineration landfill leachate after anaerobic fermentation is added to the bottom of the support plate from the water inlet port, and facultative residual sludge is inoculated from the sludge discharge port, so that short-cut denitrification bacteria existing in the facultative residual sludge can rapidly breed, and the anaerobic ammonia oxidation bacteria can be provided with nitrite, after organic matter is consumed, the mixed liquid suspended solid concentration in the residual sewage under the support plate is controlled to be not more than 5500 mg / L;

[0018] S3, the enrichment collection bag inoculated with anaerobic ammonia oxidation residual sludge is slowly put above the support plate, and repeated intermittent water feeding is conducted according to the set water feeding amount and standing time, so that the pH is controlled to be 7.0, the liquid level reaches the water outlet in the reaction tank, and automatic liquid discharge is conducted;

[0019] S4, after 30 days, the sample bag in the enrichment collection bag is salvaged by using the color of the anaerobic ammonia oxidation bacteria, and whether the product meets the standard is judged by using the intermittent sensory method, until the red anaerobic ammonia oxidation bacteria are distributed on the filler, and the bacteria culture requirement is met.

[0020] The application also relates to a use method of the anaerobic ammonia oxidation screening enriching device.

[0021] S1, first, water test is conducted on the reaction tank, and after the reaction tank is detected to be watertight, the water is discharged;

[0022] S2, the incineration landfill leachate after nitrification and denitrification is added to the bottom surface of the support plate from the water inlet port, two different types of sludge are inoculated, anaerobic residual sludge is inoculated from the sludge discharge port, and the enrichment collection bag inoculated with anaerobic ammonia oxidation residual sludge is put into the upper part of the reaction tank, and reaction is conducted for 15 days;

[0023] S3, continuously adding the incineration landfill leachate after nitrification and denitrification into the reaction tank through the water inlet, continuously discharging water through the water outlet, maintaining the hydraulic flow in the reaction tank, and controlling the hydraulic retention time to be 5-6 days and the pH to be 7.0-7.2;

[0024] S4, after 30 days, the sample bag in the enrichment collection bag is salvaged by using the color of the anaerobic ammonia oxidation bacteria, and whether the product meets the standard is judged by using the intermittent sensory method until the anaerobic ammonia oxidation bacteria of red color are distributed in the filler and the bacteria culture requirement is met.

[0025] The present application has the following technical effects relative to the prior art:

[0026] The anaerobic ammonia oxidation enrichment culture device and the use method developed by the present application can quickly realize the enrichment and screening of anaerobic ammonia oxidation bacteria, and can realize the recycling of anaerobic ammonia oxidation bacteria. BRIEF DESCRIPTION OF DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0028] Figure 1 It is a structure schematic diagram of the anaerobic ammonia oxidation screening enrichment device in the embodiments of the present application;

[0029] Figure 2 It is a structure schematic diagram of the support plate in the embodiments of the present application;

[0030] Figure 3 It is a structure schematic diagram of the cover in the embodiments of the present application;

[0031] Figure 4 It is a structure schematic diagram of the extraction rope in the embodiments of the present application;

[0032] Figure 5 It is a structure schematic diagram of the bundle port of the enrichment collection bag in the embodiments of the present application;

[0033] Figure 6 It is an effect diagram of applying the cultured anaerobic ammonia oxidation biofilm to the removal of ammonia nitrogen in homologous landfill incineration leachate in the embodiments of the present application;

[0034] In the figure: 1-reaction tank, 2-supporting plate, 3-enrichment collection bag, 4-electric hoist, 5-sample bag, 6-water inlet, 7-mud outlet, 8-water outlet, 9-bearing block, 10-hook, 11-pulling rope, 12-cofferdam, 13-external outlet, 14-crawling ladder, 15-filter hole, 16-connection hole, 17-adjustment lock, 18-cover, 19-manhole. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0036] It should be noted that, in the description of the present application, the terms "upper", "lower", "left", "right", "inner", "outer", "front", "back", "clockwise", "counterclockwise" and other terms indicating the direction or positional relationship are based on the direction or positional relationship shown in the drawings, which is only for the convenience of description, and does not indicate or imply that the device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second", "third", "fourth" are only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined with "first", "second", "third", "fourth" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise explicitly specified and limited.

[0037] In addition, it should also be noted that, in the description of the present application, unless otherwise explicitly specified and limited, the terms "set", "connected", "connected" should be broadly understood, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be connected inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0038] The purpose of the present application is to provide an anaerobic ammonia oxidation screening enrichment device and a method for using the same, in order to solve the problems existing in the prior art, so that the enrichment and screening of anammox bacteria can be quickly realized, and the anammox bacteria can be recycled.

[0039] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0040] Example 1

[0041] like Figures 1 to 5 As shown, this embodiment provides an anaerobic ammonia oxidation screening and enrichment device, including a reaction tank 1, enrichment collection bags 3, and a hoisting mechanism. The bottom of the reaction tank 1 is provided with an inlet 6 and a sludge discharge port 7, and the top is provided with an outlet 8. The reaction tank 1 is used to contain wastewater, and the enrichment collection bags 3 are submerged in the wastewater. Several enrichment collection bags 3 are hung inside the reaction tank 1. The enrichment collection bags 3 are permeable to water and air and contain packing material for cultivating anaerobic ammonia oxidizing bacteria. The hoisting mechanism is used to lift the enrichment collection bags 3 for inspection or to transport them in and out of the reaction tank 1; or, several enrichment collection bags 3 are hung on the hoisting mechanism, and the enrichment collection bags 3 are located inside the reaction tank 1. The inlet 6 is higher than the sludge discharge port 7.

[0042] As an optional solution, in this embodiment, a support plate 2 is detachably installed inside the reaction vessel 1. The support plate 2 has several filter holes 15 evenly distributed on it, and an enrichment collection bag 3 is placed on the support plate 2. The diameter of the filter holes 15 is 5mm-10mm. A bearing ring or several bearing blocks 9 are provided on the inner wall of the reaction vessel 1. The support plate 2 overlaps the bearing ring or bearing blocks 9. The bearing blocks 9 are made of high-hardness stainless steel or corrosion-resistant carbon steel. The thickness of the bearing blocks 9 is 3cm-10cm and the width is 5cm-50cm. The support plate 2 is located at 1 / 3-1 / 2 of the height of the reaction vessel 1. The support plate 2 is provided with a connection hole 16 for connecting to a lifting chain. The lifting chain is connected to a hoisting mechanism. The support plate 2 is made of high-hardness stainless steel or corrosion-resistant carbon steel, with filter holes 15 evenly distributed. The radius of the support plate 2 is 0.5-1cm smaller than that of the reaction vessel 1. Four lifting chains for maintenance are installed at one-third to one-half of the radius of the support plate 2. The lifting chains are evenly distributed on the surface of the support plate 2 to ensure the uniform distribution of lifting force and prevent overturning.

[0043] As an optional solution, in this embodiment, the enrichment collection bag 3 includes several sealable bags with a volume of 0.1m³-1m³. The enrichment collection bag 3 is made of 600g non-woven fabric. Each enrichment collection bag 3 has a drawstring channel at its opening. The drawstring channel is made of double-layer non-woven fabric, and a pair of drawstrings 11 are arranged symmetrically in opposite directions inside the drawstring channel. Each drawstring 11 has an adjusting buckle 17 at its outer end. The ends of both drawstrings 11 are used to hang on the reaction vessel 1 or the lifting mechanism. The enrichment collection bag 3 only needs to be made of a material that is water-permeable, air-permeable, and durable.

[0044] As an optional solution, the enrichment collection bag 3 in the embodiment comprises a sample bag 5 with a volume of 0.1 m³ and a plurality of culture bags with a volume greater than 0.1 m³.

[0045] As an optional solution, the filler in the embodiment comprises iron-copper modified biochar and diatomite, nanocellulose, and the ratio of iron-copper modified biochar: diatomite: nanocellulose is (0.8-1):(0.5-1):(0.2-1). Specifically, the iron-copper modified biochar is prepared as follows: rice husk charcoal is added into an equal volume of mixed FeCl3(0.8M) and CuSO4·5H2O(0.3M) mixed solution, the pH is adjusted to 5 using 0.5M HCl and 1.0M NaOH, and then the solution is placed in a constant temperature shaking incubator at 85℃, 180 r·min −1 for 24 hours, and then dried at 75℃ for 12 hours; then heated to 300℃ at a heating rate of 10℃·min −1 under N2 protection, and kept for 2 hours; then washed with ultrapure water for 3-5 times, and dried at 75℃ for 12 hours. The iron-copper modified biochar in the embodiment has a rough surface, a large specific surface area, enhanced aromaticity, strong adsorption capacity, and pH buffering resistance. As an anaerobic ammonia oxidation screening carrier, the iron-copper modified biochar can not only provide growth elements iron for anaerobic ammonia oxidation bacteria, maintain the acid-base environment of the reaction system, and promote the growth and reproduction of anaerobic ammonia oxidation bacteria, but also can adsorb anaerobic ammonia oxidation bacteria on the carrier. Using ordinary biochar as the carrier is also effective, but the anaerobic ammonia oxidation bacteria grow slowly in the case of ordinary biochar, are not easy to grow into granular form, are greatly affected by water flow, and are easy to fall off from the carrier.

[0046] As an optional solution, the reaction tank 1 in the embodiment is provided with a cover 18, the cover 18 is provided with a manhole 19 with a size of 80cm*80cm, a climbing ladder 14 is arranged on the side wall of the reaction tank 1, and the height of the climbing ladder 14 is at least the height of the reaction tank 1; a hook 10 is arranged on the inner wall top of the reaction tank 1, and the hook 10 is used for hanging the enrichment collection bag 3.

[0047] As an optional solution, a cofferdam 12 is arranged around the bottom of the reaction tank 1 along the outer wall of the reaction tank 1, the sludge discharge port 7 is arranged at the bottom of the reaction tank 1 and located in the cofferdam 12, an external discharge port 13 is arranged on the side wall of the cofferdam 12, and valves are arranged on the water inlet port 6, the water outlet port 8, the sludge discharge port 7, and the external discharge port 13.

[0048] As an optional solution, the material of the reaction tank 1 in the embodiment comprises stainless steel or carbon steel material after corrosion protection treatment, and the wall thickness of the reaction tank 1 is 1mm-10mm; the hoisting mechanism is an electric hoist 4, and the electric hoist 4 is welded to the inner wall of the top of the reaction tank 1 or the cover 18.

[0049] Example Two

[0050] The present embodiment provides a method for using an anaerobic ammonia oxidation screening enricher, based on the anaerobic ammonia oxidation screening enricher described above, and specifically includes the following steps:

[0051] S1, first test the reaction tank 1, fill the tank with water through the water inlet 6 to the water outlet 8, close all valves, and after 24-48 hours, detect that the reaction tank 1 is not leaking, then empty the water;

[0052] S2, add the anaerobically fermented incineration landfill leachate to the volume of 85%-96% below the support plate 2 of the reaction tank 1 from the water inlet 6, inoculate the facultative residual sludge from the sludge outlet 7, and the inoculation amount is 4%-15% of the volume below the support plate, at this time the liquid level in the reaction tank 1 rises to the support plate 2, and 5-10 days are waited for reaction, so that the short-cut denitrification bacteria existing in the facultative residual sludge can rapidly multiply to provide nitrite for the anaerobic ammonia oxidation bacteria, until the organic matter is basically consumed, when the MLSS concentration (mixed liquor suspended solids concentration) of the residual sewage below the support plate 2 reaches 5500 mg / L, part of the sediment enriched sludge is discharged from the sludge outlet 7, until the MLSS concentration of the residual sewage below the support plate 2 is reduced to below 3000 mg / L, and the discharge of sludge is stopped to prevent the MLSS concentration from being too high to cause sludge bulking and reduce the concentration of short-cut denitrification bacteria, thereby affecting the normal use of the equipment. The MLSS concentration is detected by a sludge concentration meter. In the present embodiment, the support plate 2 is located at about 1 / 3 of the volume of the reaction tank 1.

[0053] S3, slowly put the enrichment collection bag 3 inoculated with anaerobic ammonia oxidation residual sludge above the support plate 2, and repeat the intermittent water feeding according to the set water feeding amount and standing time, so that the pH is controlled at 7.0, the liquid level reaches the water outlet in the reaction tank, and automatic liquid discharge is performed. The water feeding amount can be 1 / 3-1 / 2 of the volume of the reaction tank 1, and specifically, 1 / 3 of the volume of the reaction tank 1 is taken as an example, the water is fed through the water inlet 6 to cover the enrichment collection bag 3 in the reaction tank 1, the enrichment collection bag 3 inoculated with anaerobic ammonia oxidation residual sludge is slowly put above the support plate 2 of the reaction tank 1, the inoculation amount is 6%-10% of the volume of the residual sewage, the water is fed from the water inlet 6 so that the water feeding amount is 1 / 3 of the volume of the reaction tank 1, and the pH is controlled at 7.0 after standing for 11-15 days; the water is fed from the water inlet 6 so that the water feeding amount is 1 / 3 of the volume of the reaction tank 1 (i.e. the liquid level in the reaction tank 1 is at 2 / 3 of the overall height of the reaction tank 1), and the pH is controlled at 7.0 after standing for 11-15 days; the intermittent water feeding is adopted, and the above water feeding amount and standing time are repeated, the liquid level in the reaction tank 1 reaches the water outlet 8, and automatic liquid discharge is performed.

[0054] In this embodiment, the facultative sludge and the anammox sludge are inoculated in different spaces in the same reactor 1, which is conducive to the cultivation of anammox bacteria and can shorten the cultivation time. The facultative sludge below the tank body can provide sustainable nitrite for the anammox sludge through short nitrification and denitrification, and the short denitrification can consume the chemical oxygen demand (COD). At the same time, the gradient nitrite is provided for the growth of anammox bacteria by batch feeding, which promotes the survival of anammox bacteria. The anammox bacteria on the support plate 2 are provided with nitrite, and the nitrite concentration gradually fades from the beginning to the end of the reaction, and from the support plate 2, the nitrite concentration gradually fades from bottom to top, which keeps the nitrite concentration below the inhibition threshold (different anammox sludge has different values, and in this embodiment, the value is about 450 mg / L). At the same time, the stable nitrite is provided for the anammox bacteria, which promotes the gradient growth of the anammox bacteria, can screen and cultivate the target anammox bacteria in a short time, and can consume the nitrogen in the water body while cultivating the anammox bacteria.

[0055] S4, using the color of anammox bacteria, the intermittent sensory method is used to judge whether the product meets the standard, which is convenient for product production and quality inspection. The specific features are as follows: after 30 days, the sample bag 5 in the enrichment collection bag 3 is pulled out of the water surface by the electric hoist 4 or manually, the sample bag 5 is opened, and the naked eye is observed. If the red bacteria cover the filler, it means that the bacteria have matured, which indicates that the sample bag 5 has completed the enrichment and cultivation of anammox bacteria and can be directly used as a strong bacteria agent. If there is no red bacteria, the sample bag 5 is locked by the adjusting lock 17 and then put back into the reactor 1 for further enrichment and cultivation. Thereafter, the sample bag 5 is pulled up every 7-10 days for observation until the red bacteria cover the filler and meet the requirements of bacterial cultivation. At this time, the collection bag can be pulled out and placed in the temporary storage area as an additional bacteria agent, which can be directly applied to other wastewater treatment anammox processes for inoculation and start-up. The facultative sludge and the anammox sludge are both from the sludge discharged from the sludge discharge port 7 of the reactor 1.

[0056] The water in this embodiment is landfill leachate, and the water is used intermittently. The pH is controlled at 7.0 to provide a better growth environment for methanogens, inhibit the growth of miscellaneous bacteria such as actinomycetes, yeasts and molds, remove organic matter, inhibit the occurrence of nitrification, facilitate short-cut denitrification and help to achieve the accumulation of nitrite. The anaerobic ammonia oxidation bacteria can be cultured while the landfill leachate is treated by incineration. Specifically, 5% of the landfill leachate is injected in the first step, and the oxygen in the incinerated landfill leachate is consumed under the action of facultative sludge, thereby providing an anaerobic environment for the anaerobic ammonia oxidation reaction in the later stage. At the same time, the consumption of organic matter in the incinerated landfill leachate is achieved through facultative oxidation, which avoids the inhibitory effect of high-concentration organic matter on anaerobic ammonia oxidation and avoids the stimulation of organic matter on the growth of denitrifying bacteria. At the same time, the short-cut denitrifying bacteria reduce nitrate nitrogen to nitrite nitrogen, thereby providing a reaction substrate for the subsequent anaerobic ammonia oxidation.

[0057] Embodiment three

[0058] The embodiment provides a use method of an anaerobic ammonia oxidation screening enriching device. The use method is based on the anaerobic ammonia oxidation screening enriching device and specifically includes the following steps.

[0059] S1, first, a water test is performed on the reaction tank 1. The tank body is filled with water through the water inlet 6 to the water outlet 8. After 24-48 hours, it is detected that the reaction tank 1 is not leaking, and then the water is drained.

[0060] S2, the incinerated landfill leachate subjected to nitrification and denitrification is added to the bottom surface of the support plate 2 through the water inlet 6, and the anaerobic residual sludge is inoculated from the sludge outlet 7. The inoculation amount is 3%-15% of the volume of the reaction tank. The enriching collection bag 3 inoculated with the anaerobic ammonia oxidation residual sludge is slowly placed above the support plate 2 of the reaction tank 1. The inoculation amount is 6%-15% of the volume of the reaction tank. The reaction lasts for 15 days. The support plate 2 is used as a boundary. The anaerobic residual sludge is inoculated below the support plate 2 to consume the residual organic matter in the incinerated landfill leachate. When the concentration of the organic matter is too high, the growth of the anaerobic ammonia oxidation bacteria is inhibited. At the same time, the dissolved oxygen in the leachate can be consumed. In order to allow part of the nitrate nitrogen that has been oxidized to occur short-cut denitrification under anaerobic conditions, a stable anaerobic environment and stable nitrite nitrogen are provided for anaerobic ammonia oxidation. The two different types of sludge, namely the anaerobic residual sludge and the anaerobic ammonia oxidation sludge, are inoculated in different spaces of the same reaction tank 1, which is beneficial to the culture of the anaerobic ammonia oxidation bacteria and can shorten the culture time. The short-cut denitrifying bacteria in the anaerobic residual sludge can rapidly reproduce and consume the chemical oxygen demand (COD).

[0061] S3, continuously adding the incineration landfill leachate after nitrification and denitrification into the reaction tank 1 through the water inlet 6, while continuously discharging water through the water outlet, maintaining the hydraulic flow in the reaction tank, and controlling the hydraulic retention time (reaction time) to be 5-13 days and the pH to be 7.0-7.2; continuously flowing through the water flow to provide gradient nitrite for the anaerobic ammonia oxidation bacteria, avoiding the inhibition of high-concentration nitrite on the anaerobic ammonia oxidation bacteria, and promoting the survival of the anaerobic ammonia oxidation bacteria. Providing nitrite for the anaerobic ammonia oxidation bacteria on the support plate 2, and from the support plate, the nitrite concentration is from high to low from bottom to top, and the nitrite concentration is maintained below the inhibition threshold value (different anaerobic ammonia oxidation sludge has different values, and the value of the present technology is about 450 mg / L), which can provide stable nitrite for the anaerobic ammonia oxidation bacteria, promote the gradient growth of the anaerobic ammonia oxidation bacteria, and can screen and cultivate target anaerobic ammonia oxidation bacteria in a short time, and realize the consumption of nitrogen in the water body while cultivating the anaerobic ammonia oxidation bacteria; the specific parameters of this step are that the hydraulic retention time is controlled to be 5-13 days, and the pH is controlled to be 7.0-7.2.

[0062] S4, using the color of the anaerobic ammonia oxidation bacteria, using intermittent sensory method to judge whether the product meets the standard, and facilitating product production quality inspection, and the specific features are as follows: after 30 days, the sample bag 5 in the enrichment collection bag 3 is pulled out of the water surface by the electric hoist 4 or manually, the sample bag 5 is opened, and naked eye observation is performed, if the red bacteria cover the filler, it indicates that the bacteria have matured, which means that the sample bag 5 is considered to have completed the enrichment and cultivation of anaerobic ammonia oxidation bacteria, and can be directly used as a strong bacteria agent, and the sealing bag is used for lifting and transferring use according to the use amount; if there is no red bacteria, the sample bag 5 is locked by the adjusting lock 17 and then put back into the reaction tank 1 for further enrichment and cultivation, and thereafter, the sample bag 5 is salvaged once every 7-10 days for observation until the red bacteria cover the filler and meet the bacteria cultivation requirements. At this time, the collection bag can be salvaged and placed in the bacteria temporary storage area as an additional bacteria agent, which can be directly applied to the inoculation and start-up of other wastewater treatment anaerobic ammonia oxidation processes. The facultative residual sludge and the anaerobic ammonia oxidation residual sludge are both from the sludge discharged from the sludge discharge port 7 of the reaction tank 1.

[0063] The use method of the embodiment can realize continuous water inlet, improve the reaction substrate contact amount in the sludge cultivation process, and promote the growth of anaerobic ammonia oxidation bacteria.

[0064] Example Four

[0065] In the embodiment, a manufacturing method of an anaerobic ammonia oxidation screening enrichment device is provided, and the manufacturing method specifically includes the following steps:

[0066] The reaction tank 1 is welded by 1 meter x (1-3) meter steel plate, and argon arc welding is used for welding. In the first step, the bottom of the enrichment collection tank is processed, the steel plate is welded into the bottom of the reaction tank 1, the bottom is fixed on the reinforced concrete foundation by bolts, and 15 cm is reserved as the outer edge on the bottom. In the second step, the tank wall is processed, the tank wall is welded on the tank bottom by argon arc welding until the required height. In the third step, the mud discharge pipe is installed by opening a hole in the tank wall bottom, the water inlet pipe is installed by opening a hole in the side wall at 4% of the height of the reaction tank 1 from the tank bottom, and the water outlet pipe is installed by opening a hole in the side wall at 3% of the height of the reaction tank 1 from the tank top. The above-mentioned water inlet pipe and water outlet pipe are made of the same material as the reaction tank 1 and are connected by argon arc welding. In the third step, the bearing block 9 is welded on the tank wall at one third of the distance from the tank bottom. First, the width is cut to be 2 times the width of the bearing block 9, and the middle position is bent to 90° by a bending machine to form an angle steel model with a certain arc, the length is cut according to the bending degree of the steel according to the standard GB / T709-2006, and then the cut bearing block 9 is welded on the tank wall. In the fourth step, the electric hoist 4 is welded on the side wall, the electricity is turned on at the same time, and then the electricity is turned off for standby after debugging.

[0067] The fifth step is to make the support plate 2. First, a whole plate is made according to the characteristics of the support plate 2, then concentric circles are drawn on the whole plate with a center point as the center, and the interval is 25-50 mm. Then the plate is divided into sectors with the center point as the center, and the intersection of the concentric circles and the sector radii is the center of the hole to be drilled. Then a small hole with a diameter of 5-10 mm is drilled on the plate with a drill. Then 2-4 maintenance lifting chains are installed on the support plate 2 at the radius of one-third to one-half of the radius, and the lifting chains are preferably made of stainless steel. The lower part is fixed on the support plate 2 by bolts. The support plate 2 is completed and ready for use. The sixth step is to make the enrichment collection bag 3. 600g of non-woven fabric is cut into a circular piece, then the prepared filler is placed, and then the non-woven fabric is welded by ultrasonic welding. The welding edge of 7-10 cm is welded to the non-woven fabric, that is, the non-woven fabric is welded and sewn by ultrasonic welding. The enrichment collection bag 3 has another hanging ear formed by a drawstring. In addition, a sample bag 5 with a size of 0.1m³ is made, and a stretchable seam is sewn at a distance of 3-5 cm from the edge. The collection bag uses double drawstring binding, and the specific structure is that two drawstrings are symmetrically distributed on both sides of the opening, and the rope penetrates the inside of the channel binding, forming a bidirectional stress structure. The binding uses a double-layer cloth processing channel, and the tail is prevented from coming off by a hard adjustment lock 17. The adjustment lock 17 is made of PE material. This structure can realize bidirectional adjustment, is suitable for different volume storage, forms an X-shaped locking structure, is not easy to loosen, and is suitable for water flow fluctuation application scenarios with a certain flow rate. When in use, the two sides of the rope are pulled to the appropriate position, and the adjustment lock 17 is fixed. When opened, the adjustment lock 17 is directly opened, and the one-key opening and closing is realized. The drawstring 11 of the binding is made into a hanging ear with a length not less than the distance from the hook to the opposite support plate 2. The color or shape is obviously different from other sealed enrichment collection bags 3, which is convenient for later observation and can open the inside of the enrichment collection bag 3. The prepared filler is placed, and the processing is completed and ready for use.

[0068] The seventh step is to process the ladder 14. First, cut the ladder 14 from the steel plate with a size of 50*25*20 cm. Then, weld the ladder 14 to the top of the tank from the bottom of the reaction tank 1. Then, cut the cold rolled steel bar to the length of 1 meter, which is one more than the number of steps. Weld the steel bar to the outer side wall of the steps. Finally, connect the upper part of the steel bar welded on the steps with a certain length of steel bar by welding. Install the steel mesh inside the railing. The ladder 14 is welded to one side of the tank body, and the outside is equipped with a handrail, which presents a spiral or vertical rise. The eighth step is to vertically weld a steel plate with a height of 5 cm-50 cm on the extended side of the bottom of the reaction tank 1 to form a cofferdam 12. According to the actual situation of the applicable site, reserve a sewage outlet on one side. The ninth step is to hoist the support plate 2 processed in the fifth step onto the bearing block 9 by using an electric hoist, and at the same time, place the chain on the hook 10 at the top of the reaction tank 1. The collection bag processed in the sixth step is placed on the support plate 2 by the electric hoist 4. The tenth step is to weld the cover 18 on the top of the reaction tank 1. The material of the cover 18 is the same as that of the reaction tank 1. When welding, the top is cut into several sectors, and the welding starts from the edge of the reaction tank 1 to the center. Thus, the entire device is processed. The production of the device using the manufacturing method of the embodiment can be completed on site, which is especially suitable for scenes where the site is limited or the required reaction tank 1 is too large to be transported conveniently.

[0069] Embodiment five

[0070] As Figure 6 shown, the cultured anaerobic ammonia oxidation biofilm is applied to the removal of ammonia nitrogen in the homologous garbage incineration leachate (the effluent from the anaerobic reactor is used as the treatment target). The CK is the control group, and the EG is the experimental group: the anaerobic ammonia oxidation enrichment bag is not added to the control group (CK), and the enrichment bag is added to the experimental group (EG). The ammonia nitrogen degradation effect of the system is shown in Figure 6 , and it can be seen from Figure 6 that the ammonia nitrogen removal rate in the leachate with the addition of the anaerobic ammonia oxidation enrichment bag is significantly higher than that in the control group. The experimental group realizes the rapid start-up of the anaerobic ammonia oxidation process within about 7 days.

[0071] In the description of the present specification, the description of the terms "one embodiment", "certain embodiments", "illustrative embodiments", "the present embodiment", "specific examples" or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiments or examples are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0072] The principles and implementation manners of the present application are described by using specific examples in the present application, and the above examples are only used for helping to understand the method of the present application and its core idea; meanwhile, for the general technical personnel in the art, according to the idea of the present application, the specific implementation manners and application ranges will be changed. In conclusion, the content of the present specification should not be understood as the limitation of the present application.

Claims

1. An anaerobic ammonia oxidation screening and enrichment device, characterized in that: The device includes a reaction tank, enrichment collection bags, and a hoisting mechanism. The reaction tank has an inlet and a sludge outlet at the bottom and an outlet at the top. The reaction tank is used to contain wastewater, and the enrichment collection bags are submerged in the wastewater. Several enrichment collection bags are suspended inside the reaction tank. The enrichment collection bags are permeable to water and air and contain packing material for cultivating anaerobic ammonia-oxidizing bacteria. The hoisting mechanism is used to lift the enrichment collection bags for inspection or to transport them in and out of the reaction tank; alternatively, several enrichment collection bags are suspended on the hoisting mechanism, and the enrichment collection bags are located inside the reaction tank. A support plate is detachably installed inside the reaction vessel. The support plate has a number of filter holes evenly distributed on it, and the enrichment collection bag is placed on the support plate. A surrounding dike is provided around the bottom of the reaction tank along the outer wall of the reaction tank. The sludge discharge port is located inside the surrounding dike. An external discharge port is provided on the side wall of the surrounding dike. Valves are provided on the water inlet, the water outlet, the sludge discharge port and the external discharge port.

2. The anaerobic ammonia oxidation screening enrichment device according to claim 1, characterized in that: The diameter of the filter holes is 5mm-10mm; a bearing ring or several bearing blocks are provided on the inner wall of the reaction vessel, and the support plate overlaps the bearing ring or the bearing blocks; the support plate is located at 1 / 3-1 / 2 of the height of the reaction vessel; the support plate is provided with a connection hole for connecting to the lifting chain, and the lifting chain is connected to the hoisting mechanism.

3. The anaerobic ammonia oxidation screening enrichment device according to claim 1, characterized in that: The enrichment collection bag includes several sealable bags with a volume of 0.1m³-1m³; the enrichment collection bag is made of 600g non-woven fabric, and each enrichment collection bag has a drawstring channel at its opening. The drawstring channel is made of double-layer non-woven fabric, and a pair of drawstrings are arranged symmetrically in opposite directions inside the drawstring channel. Each drawstring has an adjusting buckle at its outer end, and the ends of both drawstrings are used to hang on the reaction vessel or the hoisting mechanism.

4. The anaerobic ammonia oxidation screening enrichment device according to claim 1, characterized in that: The enrichment collection bag includes a sample bag with a volume of 0.1 m³ and several culture bags with a volume greater than 0.1 m³.

5. The anaerobic ammonia oxidation screening enrichment device according to claim 1, characterized in that: The filler includes iron-copper modified biochar, diatomaceous earth, and nanocellulose, and the ratio of iron-copper modified biochar:diatomaceous earth:nanocellulose is (0.8-1):(0.5-1):(0.2-1).

6. The anaerobic ammonia oxidation screening enrichment device according to claim 1, characterized in that: The reaction vessel is provided with a cover, and the cover is provided with a manhole, the manhole being 80cm*80cm in size; a ladder is provided on the side wall of the reaction vessel, the height of the ladder being at least the height of the reaction vessel; a hook is provided on the top of the inner wall of the reaction vessel, the hook being used to hang the enrichment collection bag.

7. The anaerobic ammonia oxidation screening enrichment device according to claim 1, characterized in that: The reaction vessel is made of stainless steel or corrosion-resistant carbon steel, and the wall thickness of the reaction vessel is 3cm-10cm. The hoisting mechanism is an electric hoist, which is welded to the inner wall of the top of the reaction vessel.

8. A method of using an anaerobic ammonia oxidation screening and enrichment device, based on the anaerobic ammonia oxidation screening and enrichment device according to any one of claims 1-7, characterized in that, Specifically, the steps include the following: S1. First, conduct a water test on the reaction tank. After confirming that the reaction tank does not leak, drain the water. S2, add the anaerobic fermented incineration leachate from the inlet to the bottom of the support plate of the reaction tank, and inoculate facultative sludge from the sludge discharge port to rapidly multiply the short-range denitrifying bacteria in the facultative sludge and provide nitrite for the anaerobic ammonia oxidizing bacteria. After the organic matter is consumed, control the concentration of suspended solids in the mixed liquor of the remaining wastewater below the support plate to not exceed 5500 mg / L. S3. Slowly place the enrichment collection bag inoculated with the residual sludge from anaerobic ammonia oxidation above the support plate. Repeat intermittent water intake according to the set water intake and settling time to keep the pH at 7.

0. When the liquid level reaches the outlet in the reaction tank, the liquid is automatically drained. S4. After 30 days, the sample bags in the enrichment collection bag are retrieved using the color of the anaerobic ammonia oxidizing bacteria. Intermittent sensory methods are used to determine whether the product meets the standards until the red anaerobic ammonia oxidizing bacteria cover the packing material, thus meeting the bacterial culture requirements.

9. A method of using an anaerobic ammonia oxidation screening and enrichment device, based on the anaerobic ammonia oxidation screening and enrichment device according to any one of claims 1-7, characterized in that, Specifically, the steps include the following: S1. First, conduct a water test on the reaction tank. After confirming that the reaction tank does not leak, drain the water. S2, add the incineration leachate after nitrification and denitrification to the bottom of the support plate through the inlet, inoculate two different types of sludge, inoculate anaerobic excess sludge through the sludge discharge port, and put the enrichment collection bag inoculated with anaerobic ammonia oxidation excess sludge into the upper part of the reaction tank, and react for 15 days. S3, continuously add the incineration leachate after nitrification and denitrification into the reaction tank through the inlet, while continuously discharge water from the outlet to maintain hydraulic flow in the reaction tank, and control the hydraulic flow for 5-6 days, and control the pH at 7.0-7.

2. S4. After 30 days, the sample bags in the enrichment collection bag are retrieved using the color of the anaerobic ammonia oxidizing bacteria. Intermittent sensory methods are used to determine whether the product meets the standards until the red anaerobic ammonia oxidizing bacteria cover the packing material, thus meeting the bacterial culture requirements.

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