Novel brain slice and tissue slice incubator

Abstract

The invention relates to the technical field of brain slice incubator equipment, and particularly discloses a novel brain slice and tissue slice incubator which is technically characterized by comprising a closed unit, an incubation module is fixedly connected in an inner cavity of the closed unit, and a temperature control and dissolution control mechanism penetrating into the incubation module is arranged on the right side of the top of the closed unit. According to the incubator, a stable incubation environment is constructed through a closed unit, a nylon net is arranged on a supporting plate of an incubation module to place brain slices or tissue slices, a partition plate is matched with an air inlet frame, and mixed gas is uniformly conveyed to incubation liquid through the design of an annular oxygen conveying pipe and an exhaust nozzle, so that the gas dissolving efficiency is improved, and the tissue slices are prevented from being blown by gas pressure. In the temperature control and dissolution control mechanism, a mixed gas conveying pipe conveys gas, a square hollow box is matched with a semiconductor chilling plate for refrigeration to improve the gas solubility, and meanwhile, a heating end transmits heat to incubation liquid for heating through a metal heat conducting sheet and a heat conducting pipe, so that temperature regulation and control are realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of brain slice incubator equipment, in particular to a novel brain slice and tissue slice incubator. BACKGROUND

[0002] At present, in neuroscience and brain tissue research, brain slice incubators, as key experimental equipment, are widely used in various research scenarios for maintaining the activity of brain slices or tissue slices in vitro, especially in experiments such as electrophysiological recording, calcium imaging, and drug screening. The incubation quality of brain slices directly determines the signal stability and physiological response accuracy in subsequent experiments, therefore, an incubation environment with stable gas supply, controllable temperature, good airtightness, and no damage to the structure of tissue slices is particularly important. However, the existing incubators still have many technical shortcomings in structure design and function implementation, which limit their experimental adaptability and application value.

[0003] Firstly, the existing incubation tank volume is generally small, and the diameter of the common circular tank is only 2 to 3 centimeters, which limits the number of samples that can be accommodated and can only place 2 to 3 rat brain slices, which is difficult to meet the demand of high-throughput experiments. In addition, the ventilation method is mostly one-way duct structure, and the gas enters the incubation liquid from a single point, which often causes the tissue slice to float or be disturbed due to unstable gas pressure or bubble accumulation, and even causes tissue slice damage, affecting experimental repeatability. Secondly, the traditional incubator generally uses external water bath heating for temperature control, which has long heat conduction path, slow response, and low heating efficiency, making it difficult to achieve precise regulation of the incubation liquid. Especially in experimental scenarios that require precise control of temperature changes, the temperature regulation delay and volatility of the existing equipment will inevitably cause changes in the state of the tissue slice.

[0004] In addition, the dissolution efficiency of mixed gas in the incubation liquid is also a key factor affecting the incubation effect. In the existing structure, mixed gas is released in the form of large bubbles on the liquid surface, and the gas-liquid contact area is limited, resulting in insufficient solubility of oxygen and carbon dioxide in the liquid, making it difficult to maintain stable oxygen partial pressure and pH, thereby affecting the normal metabolic function of the tissue slice. Insufficient airtightness is also a common problem, once affected by external environmental factors such as temperature and humidity fluctuations or air pollution, it is easy to affect the reliability of the experiment, and even cause sample contamination. In addition, most of the current incubators are still mechanical and passive structures, lacking real-time monitoring and feedback control of key parameters such as temperature and gas concentration, unable to automatically adjust the incubation conditions according to the state of the tissue, and basically do not have remote control or data visualization capabilities, which cannot meet the needs of modern scientific research for intelligence and traceability.

[0005] Therefore, developing a new brain slice and tissue slice incubator with a reasonable structure, uniform gas delivery, efficient temperature control, stable environment and a certain level of intelligence has become an urgent problem that needs to be solved in this technical field. Summary of the Invention

[0006] The purpose of the present invention is to solve the technical problems raised in the above background technology and provide a new brain slice and tissue slice incubator.

[0007] The above-mentioned purpose of the present invention is achieved like this:

[0008] A novel brain slice and tissue slice incubator includes a sealed unit, an incubation module is fixedly connected to the inner cavity of the sealed unit, and a temperature and dissolution control mechanism is provided on the top right side of the sealed unit and penetrates into the interior of the incubation module;

[0009] The enclosed unit includes four side panels forming a rectangular frame, a bottom panel fixed to the bottom end surfaces of the side panels, and a top panel fixed to the top end surfaces of the side panels;

[0010] The incubation module includes a support plate, a partition fixed to the right end surface of the support plate, and an air intake frame fixed to the right end surface of the partition. A nylon mesh for placing brain slices is fixedly connected to the middle and lower position of the inner cavity of the support plate, and a plurality of gas release holes are opened in a rectangular array in the middle and upper area of ​​the partition. A plurality of annular oxygen delivery pipes are connected to the left ports of the gas release holes. A plurality of exhaust nozzles are fixed in an annular array on the bottom surface of the annular oxygen delivery pipes. A U-shaped tube is provided in the inner cavity of the air intake frame, and the bottom end of the U-shaped tube is connected to the air intake frame.

[0011] The temperature and dissolution control mechanism includes a square hollow box, a mixed gas delivery pipe connected to the top port of the square hollow box, and a semiconductor refrigeration plate fixed to the left end face of the square hollow box by thermal grease and screws, and the heating end face of the semiconductor refrigeration plate is connected to a metal heat conducting plate, and the left end face of the metal heat conducting plate is connected to a plurality of heat pipes that penetrate into the top position of the inner cavity of the support plate.

[0012] Furthermore, the bottom plate, side plates and top plate form a closed rectangular space, the top of the inner side walls of the front and rear side plates are fixedly connected to two connecting blocks, and the four corners of the surface of the top plate are provided with screws that dock with the connecting blocks.

[0013] Furthermore, a row of through holes for the heating pipes to pass through are provided on the right side of the top of the top plate, and rubber sealing rings are fixed inside the through holes.

[0014] Furthermore, the mixed gas delivery pipe delivers the O2 / CO2 mixed gas to the interior of the square hollow box, and the bottom port of the square hollow box is connected to the port of the U-shaped tube penetrating to the top of the top plate.

[0015] Further, the refrigeration end face of the semiconductor refrigeration sheet is connected with the left end face of the square hollow box, and the O2 / CO2 mixed gas inside the square hollow box is cooled to improve the gas solubility.

[0016] Further, the right end face of the metal heat conduction sheet is coated with heat-conducting silicone grease between the heating end face of the semiconductor refrigeration sheet, and the heat of the semiconductor refrigeration sheet is conducted to the incubation liquid in the inner cavity of the support plate through the heat pipe for heating.

[0017] Further, the bottom corners of the support plate and the bottom corners of the air inlet frame are fixed with a plurality of supporting legs, the support plate and the partition plate form a rectangular frame with openings up and down, and the top surface of the nylon net is placed with the brain slices or tissue slices to be incubated.

[0018] Further, the connection position of the atomizer and the U-shaped tube is provided with a one-way valve for preventing the incubation liquid from flowing backward, and the top of the air inlet frame and the top surface of the top plate are provided with a circular hole for the U-shaped tube to penetrate and fix upward, and a sealing ring is installed in the circular hole.

[0019] Further, the atomizer is located below the lowermost partition plate, and the bottom surface of the nylon net and the top surface of the bottom plate have a spacing.

[0020] Further, the annular oxygen delivery tubes are C-shaped and arranged transversely up and down, have gaps between each other, and the exhaust outlets of the annular oxygen delivery tubes at the bottom are inclined downward for uniformly delivering the mixed gas to the incubation liquid inside the support plate.

[0021] Compared with the prior art, the present application has the following beneficial effects:

[0022] 1. The novel brain slice / tissue slice incubator of the present application builds a stable incubation environment through a sealed unit, the support plate of the incubation module is provided with a nylon net for placing brain slices or tissue slices, the partition plate cooperates with the air inlet frame, the annular oxygen delivery tube and the exhaust outlet are designed to uniformly deliver the mixed gas to the incubation liquid, thereby improving the gas dissolution efficiency and avoiding the gas pressure blowing the tissue slices.

[0023] 2. The temperature and dissolution control mechanism designed in the present application, the mixed gas delivery tube delivers gas, the square hollow box cooperates with the semiconductor refrigeration sheet to refrigerate and improve the gas solubility, and the heating end transmits heat to the incubation liquid through the metal heat conduction sheet and the heat pipe for heating, thereby realizing temperature regulation; the incubator of the present application provides suitable incubation conditions for brain slices or tissue slices, which helps to improve the incubation effect and the accuracy of experimental results. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1is the schematic diagram of the three-dimensional structure of the incubator of the present application;

[0025] Figure 2 is the schematic diagram of the internal structure of the incubator of the present application;

[0026] Figure 3 is the schematic diagram of the mechanism of the temperature and dissolution control mechanism in the present application;

[0027] Figure 4 is the schematic diagram of the structure of the incubation module in the present application;

[0028] Figure 5 is the schematic diagram of the structure of the annular oxygen delivery pipe in the present application;

[0029] Figure 6 is the schematic diagram of the structure of the top plate in the present application;

[0030] Figure 7 is the schematic diagram of the structure of the U-shaped pipe in the present application;

[0031] Figure 8 is the schematic diagram of the structure of the partition plate in the present application;

[0032] Figure 9 is the schematic diagram of the structure of the nylon net in the present application;

[0033] Figure 10 is the schematic diagram of the connection between the annular oxygen delivery pipe and the gas release hole in the present application.

[0034] Reference signs involved in the above figures:

[0035] 1, closed unit; 11, bottom plate; 12, side plate; 13, connecting block; 14, screw; 15, top plate; 2, temperature and dissolution control mechanism; 21, square hollow box; 22, mixed gas delivery pipe; 23, semiconductor refrigeration sheet; 24, metal heat conduction sheet; 25, heat pipe; 3, incubation module; 31, C-shaped support frame; 32, support leg; 33, nylon net; 34, annular oxygen delivery pipe; 35, gas release hole; 36, partition plate; 37, U-shaped pipe; 38, air inlet frame; 39, atomizer; 310, air outlet nozzle. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical solutions and advantages of the present application more clear and explicit, the present application is further described in detail below in combination with the figures and examples. It should be understood that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0037] The implementation of the present application is described in detail below in combination with specific examples.

[0038] The same or similar reference numerals in the drawings of the embodiments correspond to the same or similar components; in the description of the present application, it is understood that if the orientations or positional relationships indicated by the terms "upper", "lower", "left", "right" and the like are based on the orientations or positional relationships shown in the drawings, they are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, therefore the terms describing the positional relationship in the drawings are only used for exemplary illustration, and cannot be understood as a limitation on the present application, for those skilled in the art, the specific meanings of the above terms can be understood according to the specific circumstances.

[0039] Referring to Figures 1-10 The preferred embodiment provided by the present application is shown.

[0040] Embodiment one: the scheme of the present embodiment provides a new brain slice and tissue slice incubator, the main structure of which includes airtight unit 1, temperature and dissolution control mechanism 2 and incubation module 3. Among them, the airtight unit 1 is surrounded by four side plates 12, bottom plate 11 and top plate 15 to form a rectangular frame, the top of the side plate 12 is fixedly connected with the screw 14 at the four corners of the top plate 15 through the connecting block 13, forming a closed space. The support plate 31 of the incubation module 3 is fixed in the airtight unit 1 through the bottom support 32, and the nylon net 33 is laid in the lower position of the inner cavity for placing brain slices or tissue slices. The partition plate 36 is fixed on the right side of the support plate 31, a plurality of gas release holes 35 are opened on it, and it is connected to the U-shaped tube 37 in the gas inlet frame 38 through the annular oxygen delivery pipe 34. The top end of the U-shaped tube 37 penetrates the top plate 15 and is connected with the bottom of the square hollow box 21 of the temperature and dissolution control mechanism 2. The mixed gas delivery pipe 22 delivers O2 / CO2 mixed gas to the square hollow box 21, the refrigeration end face of the semiconductor refrigeration sheet 23 is attached to the left side of the square hollow box 21, and the solubility is improved by reducing the gas temperature; the heating end face transmits heat to the incubation liquid in the inner cavity of the support plate 31 through the metal heat conduction sheet 24 and the heat conduction pipe 25, realizing temperature control. The through hole is designed on the right side of the top plate 15 to facilitate the penetration of the heat conduction pipe 25, and the rubber sealing ring is installed to prevent gas leakage.

[0041] Example two: This embodiment is based on the implementation of example one, specifically the optimization scheme of gas distribution structure. Specifically: the annular oxygen delivery pipe 34 adopts a three-layer C-shaped pipe design, each layer is arranged transversely and offset from each other, the bottom exhaust nozzle 310 is adjusted to an inclination angle of 30° downward, ensuring that the mixed gas is uniformly diffused into the incubation liquid in the form of laminar flow. The gas inlet frame 38 is additionally provided with an atomizer 39 at the connection with the U-shaped pipe 37, and a one-way valve is arranged at the output end of the atomizer 39 to prevent the incubation liquid from flowing back. The atomizer 39 is connected with the U-shaped pipe 37 through a micro air pump, which can reduce the pressure of gas release when the gas is released, improve the gas dissolution efficiency, and at the same time avoid the tissue pieces being blown by the gas with large pressure. The nylon net 33 is made of medical grade material with a pore size of 50 μm, and the surface is treated with hydrophilic treatment to enhance the stability of the brain slice adhesion.

[0042] Example three: The scheme of this embodiment is an implementation scheme for the dynamic demand of temperature control. The PID temperature control system is designed in the temperature control and dissolution mechanism 2. The current of the semiconductor refrigeration sheet 23 is controlled by the PWM speed regulation module, the PT100 temperature sensor is integrated on the surface of the metal heat conduction sheet 24, and the incubation liquid temperature is fed back to the controller in real time. When the temperature deviates from the set value ±0.5℃, the system automatically adjusts the power of the refrigeration sheet, and realizes rapid compensation through the heat conduction efficiency of the heat pipe 25. The rubber sealing ring in the through hole of the top plate 15 is upgraded to high-temperature-resistant silicone material, which cooperates with the heat-conducting silicone grease coating on the surface of the heat pipe 25 to reduce the thermal resistance. In addition, a heat preservation interlayer is additionally arranged on the inner side of the side plate 12 of the sealed unit 1, which is filled with polyurethane foam to reduce the influence of environmental temperature fluctuation on the incubation process.

[0043] Example four: This embodiment integrates the functions of examples one to three, and extends the following features: the top plate 15 of the sealed unit 1 is additionally provided with a pressure balance valve, which is connected with the external air circulation system through a micro air pump to maintain the stability of the internal cavity pressure; a magnetic stirring sub is arranged in the inner cavity of the bottom of the support plate 31 of the incubation module 3, which cooperates with the rotating magnetic field generator below the top plate 15 to realize low-speed stirring of the incubation liquid and avoid local concentration gradient; a gas concentration sensor is additionally arranged in the square hollow box 21 of the temperature control and dissolution mechanism 2 to monitor the O2 / CO2 ratio in real time, and automatically adjust the mixed gas delivery ratio through the electromagnetic valve; the overall structure adopts modular design, and the sealed unit 1, the incubation module 3 and the temperature control and dissolution mechanism 2 are connected through quick-release interfaces for easy maintenance and function expansion.

[0044] Example five: This embodiment is for the portable needs of the experimental scene, in this embodiment, the compact design is adopted: the side plate 12 and the top plate 15 of the closed unit 1 are made of aluminum alloy material, the thickness is reduced to 2mm, and the overall size is compressed to 200mmx150mmx100mm. The inner cavity depth of the support plate 31 of the incubation module 3 is adjusted to 15mm, the area of the nylon net 33 is reduced to 50mmx50mm, and 1-2 brain slices can be incubated at a time. The power of the semiconductor refrigerating sheet 23 of the temperature and dissolution control mechanism 2 is reduced to 10W, the volume of the square hollow box 21 is reduced to 50mL, the mixed gas delivery pipe 22 is integrated with a micro gas pump and a gas tank, and supports offline operation for 2 hours. All components are integrated in the portable box through the folding support, the weight is controlled within 3kg, and the needs of the field or mobile laboratory are met.

[0045] Example six: This embodiment is an optimized implementation based on example two, and a parallel double-path gas regulation structure is implemented in this embodiment. The specific implementation is as follows:

[0046] On the basis of the original single-path U-shaped tube gas supply structure, a set of independent parallel auxiliary gas inlet channels are newly designed in the gas inlet frame 38, and the O2 / CO2 mixed gas from the mixed gas delivery pipe 22 is simultaneously delivered to the two channels through a Y-shaped gas mixing distributor. One channel is connected to the original U-shaped tube 37 and supplies gas through the atomizer 39; the other channel is connected to the newly added ring-shaped gas supply coil, which is arranged around the periphery of the support plate 31 of the incubation module 3, and a plurality of micropore release openings are designed on it, the diameter of the release opening is designed to be within 0.3mm, and it is arranged at 45° inward and downward, which is used to supplement the concentration gradient unevenness problem that may be caused by edge diffusion in the central area gas dissolution process.

[0047] At the same time, in order to further improve the dynamic balance of the two gas supply paths, micro-pressure control valves are arranged on both sides of the Y-shaped gas mixing distributor, and the gas flow rates of the two channels are adjusted in real time by the control system according to the gas concentration distribution inside the incubation liquid, so as to keep the O2 / CO2 concentration in the incubation space balanced.

[0048] In addition, the nylon net 33 adopts a double-layer structure design in this embodiment, the upper layer is a 50μm hydrophilic medical net, and the lower layer is a relatively thick support grid (pore size 300μm), which enhances the overall bearing capacity and gas passing space; and a temperature and gas concentration integrated probe is embedded in the edge of the support plate 31, which is used to synchronously collect key environmental parameters of the incubation area, realize closed-loop monitoring and automatic adjustment of the incubation environment.

[0049] Embodiment seven: This embodiment is based on the optimization scheme of ultrasonic micro-disturbance assisted oxygen dissolution and tissue slice adaptability. The embodiment is an optimized implementation based on embodiment three, specifically: by adding a low-frequency ultrasonic oscillation unit below incubation module 3 to further improve gas dissolution efficiency and enhance the adaptability of the system to various types of tissue slices.

[0050] The specific structural design is: an ultrasonic oscillation sheet (frequency set to 40 kHz, power 2 W) is installed at the central position of the bottom plate 11 of the sealed unit 1, which is connected with the control circuit and can periodically excite the incubation liquid in the inner cavity of the support plate 31 to generate a micro-disturbance wave field. After the mixed gas is released through the exhaust nozzle 310, the acoustic disturbance in the liquid promotes bubble breaking and gas-liquid interface renewal, thereby improving the instantaneous dissolution rate of O2 / CO2 in the incubation liquid. This method is especially suitable for brain regions or slices with large thickness and sensitive to oxygen supply.

[0051] To adapt to different sizes and thicknesses of brain slices or tissue slices, a detachable tissue adaptation frame is added above the nylon net 33, which is embedded with a medical silicone cushion and has several limiting grooves. The adaptation size range is 20mm×20mm to 50mm×50mm. This structure can not only firmly fix different tissue slices, but also ensure sufficient contact interface between gas and liquid. The material of the adaptation frame is inert silicone, which does not interfere with the activity of the tissue.

[0052] In addition, to ensure the stability of the tissue slice under the action of ultrasonic disturbance, an elastic limiting ring is added to the edge of the support plate 31 to prevent the tissue slice from drifting or dislocating due to liquid disturbance.

[0053] This embodiment has been experimentally verified in actual application. Under the conditions of maintaining temperature at 37±0.2℃ and stable gas concentration, the oxygen dissolution efficiency of the tissue slice is increased by about 18% under ultrasonic excitation compared to the non-excited state, and the stability of different shaped tissue slices is significantly improved, further expanding the adaptability of the device in various experimental scenarios.

[0054] Embodiment eight: This embodiment is an optimization scheme integrating remote environmental monitoring and intelligent early warning functions. The scheme of this embodiment is an extension design based on embodiment four, aiming at the needs of environmental parameter monitoring, data remote acquisition and fault warning during the operation of the incubator, and proposes an information-enhanced structure implementation. The specific implementation scheme is as follows:

[0055] An environmental perception module is integrated on the side plate 12 of the sealed unit 1. The module includes a temperature sensor, a gas concentration sensor (for O2 / CO2 ratio), a pH electrode and a humidity sensor, and all data are integrated through a micro data acquisition control board (MCU). The data acquisition period is set to 1 second, and all collected data are transmitted to the built-in Wi-Fi communication module through the serial port.

[0056] The Wi-Fi module adopts an ESP32 chip and supports real-time communication with a remote database or a host computer platform. The platform interface supports data visualization display, historical record backtracking, and parameter threshold setting. When the detection value exceeds the set range (for example, the incubation liquid temperature deviates by ±1.0℃, or the O2 concentration is lower than the set value by 20%), the system immediately triggers a local audible and visual alarm, and pushes an abnormality reminder through an APP or a PC client, so as to facilitate researchers to timely adjust parameters or terminate the experiment.

[0057] To ensure data security, the communication module is matched with an AES encryption protocol for transmission, so as to avoid interception or tampering of data during Wi-Fi transmission.

[0058] In addition, to adapt to the offline or field experiment scene, the control board in this embodiment has a local storage function, can record 24 hours of continuous data without network, and automatically upload after the network is restored, thereby guaranteeing the integrity of experimental data.

[0059] The scheme of this embodiment is suitable for high requirement experiments or remote management scenes, and has the advantages of enhancing the intelligent level of the system and improving the convenience of user operation.

[0060] Working principle: when the novel brain slice / tissue slice incubator is used, first, the brain slice or tissue slice to be incubated is placed on the top surface of the nylon net 33 at the lower position in the inner cavity of the support plate 31, at this time, the bottom plate 11, the side plate 12 and the top plate 15 form a closed rectangular space to provide a closed environment for incubation, the connecting block 13 at the top of the inner side wall of the front and rear side plates 12 cooperates with the screws 14 at the four corners of the surface of the top plate 15 to ensure the stable connection of the top plate 15 and the side plate 12; the mixed gas conveying pipe 22 conveys the mixed gas of O2 / CO2 to the inside of the square hollow box 21, the bottom port of the square hollow box 21 is connected with the port of the U-shaped pipe 37 penetrating through the top of the top plate 15, so that the mixed gas can enter the U-shaped pipe 37.

[0061] The refrigeration end surface of the semiconductor refrigeration sheet 23 is connected with the left end surface of the square hollow box 21, the O2 / CO2 mixed gas in the square hollow box 21 is cooled to improve the solubility of the gas, at the same time, the heating end surface of the semiconductor refrigeration sheet 23 is connected with the metal heat conduction sheet 24, the left side end surface of the metal heat conduction sheet 24 is coated with heat-conducting silicone grease between the heating end surface of the semiconductor refrigeration sheet 23, the left side end surface of the metal heat conduction sheet 24 is connected with a plurality of heat pipes 25 penetrating through the top of the support plate 31, the heat pipes 25 penetrate into the through hole provided at the top right side of the top plate 15, the rubber sealing ring fixed in the through hole plays a sealing role, and the heat pipes 25 conduct the heat of the semiconductor refrigeration sheet 23 to the incubation liquid in the inner cavity of the support plate 31 for heating, so as to adjust the temperature of the incubation liquid.

[0062] U-shaped pipe 37 is transported to the inner cavity of the intake frame 38, the connection position of the atomizer 39 in the inner cavity of the intake frame 38 is provided with a one-way valve, which is used to prevent the backflow of the incubation liquid, and the top of the intake frame 38 and the top surface of the top plate 15 are provided with a circular hole for the upward penetration and fixation of the U-shaped pipe 37, and the inside of the circular hole is provided with a sealing ring, the atomizer 39 is located below the lowermost partition plate 36, which can reduce the pressure of the gas release when the gas is released, improve the gas dissolution efficiency, and avoid the tissue pieces being blown by the gas with large pressure; after the mixed gas comes out of the atomizer 39, it enters the rectangular frame with openings formed by the support plate 31 and the partition plate 36, a plurality of gas release holes 35 are arranged in a rectangular array in the middle and upper areas of the partition plate 36, a plurality of annular oxygen delivery pipes 34 are connected to the left side ports of the gas release holes 35, the annular oxygen delivery pipes 34 are C-shaped and arranged vertically, and have gaps between each other, a plurality of exhaust nozzles 310 are fixed in an annular array on the bottom surface of the annular oxygen delivery pipe 34, the gas outlet ports of the exhaust nozzles 310 are inclined downward, the mixed gas enters the annular oxygen delivery pipe 34 through the gas release hole 35, and is then uniformly delivered to the incubation liquid in the support plate 31, and is fully mixed with the incubation liquid to provide a suitable incubation environment for the brain slices or tissue pieces, the bottom surface of the nylon net 33 and the top surface of the bottom plate 11 have a spacing, which facilitates the circulation of the incubation liquid and the diffusion of the gas, and a plurality of supporting legs 32 are fixed to the bottom corners of the support plate 31 and the bottom corners of the intake frame 38, which serve as supports.

[0063] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement and improvement within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A novel brain slice and tissue slice incubator, comprising a closed unit (1), characterized in that: An incubation module (3) is fixedly connected to the inner cavity of the closed unit (1), and a temperature and dissolution control mechanism (2) is provided on the top right side of the closed unit (1) and extends into the interior of the incubation module (3); The enclosed unit (1) comprises four side panels (12) forming a rectangular frame, a bottom panel (11) fixed to the bottom end surfaces of the side panels (12), and a top panel (15) fixed to the top end surfaces of the side panels (12); The incubation module (3) includes a support plate (31), a partition (36) fixed to the right end face of the support plate (31), and an air intake frame (38) fixed to the right end face of the partition (36); a nylon mesh (33) for placing brain slices is fixedly connected to the middle and lower position of the inner cavity of the support plate (31); a plurality of gas release holes (35) are provided in a rectangular array in the middle and upper area of ​​the partition (36); a plurality of annular oxygen delivery pipes (34) are connected to the left end of the gas release hole (35); a plurality of exhaust nozzles (310) are fixed in an annular array on the bottom surface of the annular oxygen delivery pipe (34); a U-shaped pipe (37) is provided in the inner cavity of the air intake frame (38); and the air intake frame (38) is connected to the bottom end of the U-shaped pipe (37); The temperature control and dissolution control mechanism (2) comprises a square hollow box (21), a mixed gas delivery pipe (22) connected to the top port of the square hollow box (21), and a semiconductor refrigeration plate (23) fixed to the left end face of the square hollow box (21) by means of thermal grease and screws, wherein the heating end face of the semiconductor refrigeration plate (23) is connected to a metal heat conducting plate (24), and the left end face of the metal heat conducting plate (24) is connected to a plurality of heat pipes (25) penetrating to the top position of the inner cavity of the support plate (31).

2. A novel brain slice and tissue slice incubator according to claim 1, characterized in that: The bottom plate (11), the side plates (12) and the top plate (15) form a closed rectangular space, the tops of the inner side walls of the front and rear side plates (12) are fixedly connected to two connecting blocks (13), and the four corners of the surface of the top plate (15) are provided with screws (14) that are connected to the connecting blocks (13).

3. A novel brain slice and tissue slice incubator according to claim 1, characterized in that: A row of through holes for the heat supply pipes (25) to pass through are provided on the right side of the top of the top plate (15), and rubber sealing rings are fixed inside the through holes.

4. A novel brain slice and tissue slice incubator according to claim 1, characterized in that: The mixed gas delivery pipe (22) delivers the O2 / CO2 mixed gas to the interior of the square hollow box (21), and the bottom port of the square hollow box (21) is connected to the port of the U-shaped tube (37) penetrating to the top of the top plate (15).

5. A novel brain slice and tissue slice incubator according to claim 1, characterized in that: The cooling end surface of the semiconductor refrigeration plate (23) is connected to the left end surface of the square hollow box (21), and the gas solubility is improved by cooling the O2 / CO2 mixed gas inside the square hollow box (21).

6. A novel brain slice and tissue slice incubator according to claim 1, characterized in that: Thermal grease is coated between the right end surface of the metal heat conducting plate (24) and the heating end surface of the semiconductor refrigeration plate (23), and is used to conduct the heat of the semiconductor refrigeration plate (23) to the incubation liquid in the inner cavity of the support plate (31) through the heat pipe (25) for heating.

7. A novel brain slice and tissue slice incubator according to claim 1, characterized in that: Several legs (32) are fixed to the bottom corners of the support plate (31) and the bottom corners of the air inlet frame (38). The support plate (31) and the partition (36) form a rectangular frame with openings at the top and bottom. Brain slices or tissue slices to be incubated are placed on the top surface of the nylon mesh (33).

8. The novel brain slice and tissue slice incubator according to claim 1, characterized in that: A one-way valve is provided at the connection position between the atomizer (39) and the U-shaped tube (37) to prevent the incubation liquid from flowing back, and the top of the air intake frame (38) and the top surface of the top plate (15) are provided with circular holes for the U-shaped tube (37) to pass through and be fixed upward, and sealing rings are installed inside the circular holes.

9. The novel brain slice and tissue slice incubator according to claim 1, characterized in that: The atomizer (39) is located below the lowermost partition (36), and there is a gap between the bottom surface of the nylon mesh (33) and the top surface of the bottom plate (11).

10. The novel brain slice and tissue slice incubator according to claim 1, characterized in that: The annular oxygen delivery tubes (34) are all C-shaped and arranged horizontally up and down, with gaps between the annular oxygen delivery tubes (34), and the exhaust nozzles (310) at the bottom of the annular oxygen delivery tubes (34) are all tilted downward to uniformly deliver the mixed gas to the incubation liquid inside the support plate (31).

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