Method for detecting lucid ganoderma component in leather

Abstract

The invention belongs to the technical field of detection and analysis, and discloses a method for detecting a lucid ganoderma component in leather. According to the method, the to-be-detected leather resin is centrifugally separated by using the organic solvent, so that the interference of the leather resin on the detection of the ganoderma lucidum components is avoided, and the supernate is detected by using Fourier transform infrared spectroscopy, so that the qualitative characterization of the ganoderma lucidum components is realized. Meanwhile, according to the solubility characteristic of the added lucid ganoderma, a weight method (for example, the addition form of the lucid ganoderma is lucid ganoderma superfine powder) and a high performance liquid chromatography (for example, the addition form of the lucid ganoderma is lucid ganoderma fermentation water-based treatment liquid or lucid ganoderma strain culture) are adopted for detecting precipitates and concentrated supernate respectively; the quantitative detection and analysis of the lucid ganoderma components are realized. The quantitative detection method has the advantages of high accuracy and good repeatability, the intra-day repeatability is in the range of 2.55%-4.44%, and the inter-day repeatability is in the range of 2.03%-5.42%; and the measured value deviation ranges from 2.88% to 5.29%.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of detection analysis, and particularly relates to a detection method of ganoderma lucidum components in leather. BACKGROUND

[0002] Bio-based materials are low-carbon, environmentally friendly green materials. Bio-based materials refer to a new type of materials prepared by biological, chemical, physical and other means using renewable biomass or raw materials prepared by biological methods, such as bio-based plastics, biodiesel, bio-based chemical fibers, bio-based rubber, coatings, and additives. At present, direct use of biomass raw materials has been researched by various industries, and products rich in biomass raw materials have been put into use in the market.

[0003] Since ancient times, ganoderma lucidum has been regarded as a magical herb, a lucky herb and a divine herb, and has rich cultural connotations and good implications. The main components of ganoderma lucidum fruiting bodies are lignin and cellulose, and contain active components such as ganoderma lucidum polysaccharides and ganoderma lucidum triterpenes, which have health functions. When mixed in leather, it will emit a faint ganoderma lucidum odor, providing users with a pleasant experience. Therefore, ganoderma lucidum bio-based leather has emerged. The addition form of the ganoderma lucidum component of the ganoderma lucidum bio-based leather is various, mainly by directly adding ganoderma lucidum fermentation aqueous treatment liquid, ganoderma lucidum ultrafine powder, and ganoderma lucidum strain culture, but the addition amount should not be too much, about 0.1‰-2% of the weight of the leather compact layer, otherwise it will have a negative impact on the material properties of the leather such as wear resistance, folding resistance and environmental resistance.

[0004] Automotive leather mainly includes polyvinyl chloride (PVC) leather, polyurethane (PU) leather and PU microfiber leather. In material analysis applications, the main components are usually detected. Among them, the main components in the resin layer of PVC leather are PVC and plasticizer, and the main components in the resin layer of PU leather are PU and solvent, and the addition amount of other substances (such as ganoderma lucidum) is very small. Therefore, the conventional infrared chromatography, gas chromatography mass spectrometry and other detection means can only detect the main materials of the leather. At the same time, the production process of automotive leather mainly includes calendering method and veneering method, and the forming and embossing temperature is between 150-220℃, and the active components of ganoderma lucidum are difficult to withstand such high process temperature and are easy to lose activity.

[0005] Therefore, due to the multiple influencing factors such as low content of ganoderma lucidum components in leather and easy loss of activity, it is extremely difficult to separate and detect the ganoderma lucidum components added in the leather. SUMMARY The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides a detection method of ganoderma lucidum components in leather, which can not only realize the qualitative characterization of ganoderma lucidum components in leather, but also can be quantitatively detected, and has high accuracy and reliability. To solve the above technical problems, the first aspect of the present application provides a method for detecting ganoderma lucidum components in leather, comprising the following steps: (1) A leather resin to be tested with a weight of M1 is dissolved in an organic solvent, centrifuged to obtain supernatant and precipitate; The raw material of the leather resin to be tested includes ganoderma lucidum; (2) The supernatant is subjected to Fourier transform infrared spectroscopy detection to determine whether there is ganoderma lucidum cellulose in the supernatant; When the ganoderma lucidum is not dissolved or slightly dissolved in the organic solvent, the precipitate is detected by weight method; The detection steps of the weight method include: the precipitate is pickled, and then the insoluble substance is washed and dried to obtain ganoderma lucidum; The weight of the ganoderma lucidum is weighed and recorded as M2, and the content of the ganoderma lucidum component in the leather resin to be tested is calculated by (M2 / M1) x 100%; When the ganoderma lucidum can be dissolved in the organic solvent, the supernatant is detected by high performance liquid chromatography; The detection steps of the high performance liquid chromatography include: the supernatant is concentrated, redissolved and then subjected to full spectrum scanning; The content of the ganoderma lucidum component in the leather resin to be tested is calculated according to the response result ratio through liquid phase analysis with ganoderma lucidum acid standard sample.

[0006] Specifically, the present application first uses an organic solvent to centrifuge the leather resin to be tested to separate the ganoderma lucidum component, avoiding the interference of the leather resin on the detection of the ganoderma lucidum component, and uses Fourier transform infrared spectroscopy to detect the supernatant, realizing the qualitative characterization of the ganoderma lucidum component.

[0007] Meanwhile, according to the diversity of the addition of ganoderma lucidum component in leather, the present application uses two specific detection methods to detect the supernatant and the precipitate obtained by centrifugal separation respectively according to the solubility characteristics of the added ganoderma lucidum, so as to realize the quantitative detection and analysis of the ganoderma lucidum component. When the added ganoderma lucidum component (such as ganoderma lucidum ultrafine powder) is not dissolved or slightly dissolved (20℃, solubility in 100g organic solvent is 0.01-1g) in the organic solvent, the precipitate is detected by weight method; When the added ganoderma lucidum component (such as ganoderma lucidum fermentation aqueous treatment liquid and / or ganoderma lucidum strain culture) can be dissolved in the organic solvent, the concentrated supernatant is detected by high performance liquid chromatography.

[0008] In some embodiments of the present application, after the centrifugal separation in step (1), the precipitate is washed with an organic solvent, and the liquid after washing is combined into the supernatant.

[0009] In some embodiments of the present application, in step (1), the organic solvent is selected from at least one of tetrahydrofuran, N,N-dimethylformamide, dichloroethane and cyclohexane.

[0010] In some embodiments of the present application, in step (1), the mass-volume ratio of the leather resin to be tested to the organic solvent is (2-20) g:100 mL.

[0011] In some embodiments of the present application, in step (1), the process conditions of the centrifugal separation are as follows: centrifugation is carried out at a speed of 4000-8000 rpm for 5-10 minutes.

[0012] In some embodiments of the present application, in step (1), the leather resin to be tested does not contain a base cloth. If the leather resin to be tested has a base cloth, the base cloth is removed by soaking the leather resin in acetone, and only the resin layer is retained.

[0013] In some embodiments of the present application, in step (1), the leather resin to be tested includes automotive leather selected from any one of PVC leather, PU leather, and PU microfiber leather.

[0014] In some embodiments of the present application, in step (1), the leather resin to be tested is dissolved in the organic solvent under ultrasonic heating, and the temperature of the ultrasonic heating is 40-80°C to accelerate the dissolution.

[0015] In some embodiments of the present application, in step (1), the ganoderma is selected from at least one of ganoderma micro powder, ganoderma fermentation aqueous treatment liquid, and ganoderma strain culture. The ganoderma micro powder contains substances such as cellulose, hemicellulose, and lignin that are not easily dissolved in the organic solvent, and the ganoderma components mainly exist in the precipitate. Therefore, the weight method can be used to quantitatively detect the ganoderma in the precipitate. The ganoderma fermentation aqueous treatment liquid and the ganoderma strain culture can be dissolved in the organic solvent, and the ganoderma components mainly exist in the supernatant. Therefore, the supernatant can be extracted and concentrated, and then the high-performance liquid chromatography method can be used to quantitatively detect the ganoderma.

[0016] In some embodiments of the present application, in step (2), the Fourier transform infrared spectrum detection includes the following steps: dropping the supernatant on potassium bromide tablets, and detecting after completely drying. Since the active components of ganoderma may be inactivated during the processing, the Fourier transform infrared spectrum test can be used to determine the presence of the cellulose component of ganoderma, thereby realizing the qualitative detection of ganoderma after centrifugal separation.

[0017] In some embodiments of the present application, in step (2), the precipitate further includes a drying step before acid washing, and the drying temperature is 40-60°C.

[0018] In some embodiments of the present application, in step (2), the acid washing uses a hydrochloric acid solution with a concentration ratio of (0.5-1.5):10, i.e. a hydrochloric acid solution prepared by mixing hydrochloric acid and water in a volume ratio of (0.5-1.5):10, and the acid washing is mainly used to remove calcium carbonate in the precipitate.

[0019] In some embodiments of the present application, in step (2), the composition of the calcium carbonate is determined by thermal gravimetric analysis of the insoluble matter. The thermal gravimetric analysis is performed at a temperature range of 20-800℃ and a temperature rising rate of 5-10℃ / min.

[0020] In some embodiments of the present application, in step (2), after the acid washing, the insoluble matter is washed with deionized water to remove excess hydrochloric acid solution, and then dried at 40-60℃.

[0021] In some embodiments of the present application, in step (2), the concentration method includes rotary evaporation.

[0022] In some embodiments of the present application, in step (2), the solvent for the re-dissolution includes chromatographic grade methanol.

[0023] In some embodiments of the present application, in step (2), the ganoderic acid standard sample includes at least two of ganoderic acid A, ganoderic acid B, ganoderic acid D and ganoderic acid F.

[0024] In some embodiments of the present application, in step (2), the chromatographic conditions of the high performance liquid chromatography include: the chromatographic column uses octadecyl-bonded silica gel as the filler, the column temperature is 25-35℃, the mobile phase A is acetonitrile, the mobile phase B is phosphoric acid aqueous solution, the detection wavelength is 250-260nm, and a gradient elution program is used.

[0025] The above technical solutions of the present application have at least the following technical effects or advantages compared with the prior art: (1) The present application uses an organic solvent to centrifugally separate the leather resin to be tested, avoids the interference of the leather resin on the detection of the ganoderic acid components, and uses Fourier transform infrared spectroscopy to detect the supernatant, thereby realizing the qualitative characterization of the ganoderic acid components.

[0026] (2) The present application detects the precipitate and the concentrated supernatant by weight method (e.g. the ganoderic acid is added in the form of ganoderic acid ultra-fine powder) and high performance liquid chromatography (e.g. the ganoderic acid is added in the form of ganoderic acid fermentation aqueous treatment liquid and / or ganoderic acid strain culture) according to the solubility characteristics of the added ganoderic acid, thereby realizing the quantitative detection and analysis of the ganoderic acid components. The quantitative detection has the advantages of high accuracy and good repeatability, wherein: the intra-day repeatability is in the range of 2.55%-4.44%, the inter-day repeatability is in the range of 2.03-5.42%, and the measurement deviation is between 2.88-5.29%. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 FTIR comparison spectra of the supernatant and Ganoderma lucidum cellulose in Example 1; Figure 2 is the TGA curve of the precipitate in Example 1; Figure 3 FTIR comparison spectra of the supernatant and Ganoderma lucidum cellulose in Example 2; Figure 4 1 is a HPLC chromatogram of the supernatant and ganoderic acid standard sample in Example 2; Figure 5 FTIR comparison spectra of pure Ganoderma lucidum powder and PVC resin film and Ganoderma lucidum PVC resin film of Comparative Example 1; Figure 6 FTIR comparison spectra of pure Ganoderma lucidum powder and PU resin film and Ganoderma lucidum PU resin film of Comparative Example 2; Figure 7 This is the total ion current diagram of the thermal cracking analysis of Comparative Example 3; Figure 8 It is the high performance liquid chromatogram of the supernatant of Comparative Example 4. DETAILED DESCRIPTION

[0028] The present invention is described in detail below with reference to the examples to facilitate understanding of the present invention by those skilled in the art. It is necessary to point out that the examples are only used to further illustrate the present invention and are not to be construed as limiting the scope of protection of the present invention. Non-essential improvements and adjustments made to the present invention by those skilled in the art based on the above-mentioned invention should still fall within the scope of protection of the present invention. At the same time, the raw materials mentioned below that are not described in detail are all commercially available products; the process steps or preparation methods that are not mentioned in detail are all process steps or preparation methods known to those skilled in the art.

[0029] Example 1 A method for detecting ganoderma lucidum components in automotive leather, wherein the automotive leather is PVC leather containing ganoderma lucidum components, and the PVC leather contains a base fabric, and the detection method comprises the following steps: Dissolution and separation of the sample to be tested: (1) Soak the PVC leather in acetone. After 30 seconds, the base fabric is separated from the resin. Remove the base fabric and retain the resin layer as the sample to be tested.

[0030] (2) Weigh 5 g of the sample to be tested, cut it into small pieces less than 5 mm, put it in a glass beaker, and soak it in 60 mL of tetrahydrofuran at room temperature until the resin is completely dissolved.

[0031] (3) centrifuge the dissolved sample to be tested at a speed of 5000 r / min for 10 min, separate the supernatant and the precipitate, and wash the precipitate with tetrahydrofuran for 3 times, each time using 10 mL of tetrahydrofuran, and combine the washed liquid to the supernatant.

[0032] (4) continue to centrifuge the supernatant at a speed of 5000 r / min for 10 min, repeat step (3) to treat the supernatant and the precipitate, and obtain the treated supernatant and the precipitate.

[0033] Qualitative detection and analysis of the supernatant: The potassium bromide was pressed into a tablet, and then 0.2 mL of the supernatant prepared in step (4) was dropped onto the tablet, and after completely drying, Fourier transform infrared spectrum test was performed thereon, and the results are shown in Figure 1 From Figure 1 it can be seen that the main spectral peaks indicate that the main component is PVC, and the characteristic peaks of ganoderma cellulose are around 1120 cm -1 and 1020 cm -1 .

[0034] Quantitative detection and analysis of the precipitate: (5) collect the precipitate prepared in step (4), and dry it at 40℃, and after completely drying, weigh it on a balance, and the weight is 0.238 g. Then perform thermogravimetric analysis, the temperature time range is 20-800℃, first in nitrogen atmosphere, then switch to air atmosphere, the heating rate is (5-10) ℃ / min, and the results are shown in Figure 2 From Figure 2 it can be seen that the weight loss before 474℃ is mainly due to the additives in PVC, and the weight loss at 554℃ and 749℃ is mainly due to calcium carbonate.

[0035] (6) dissolve all the insoluble substances prepared in step (5) in 20 mL of hydrochloric acid solution with a concentration ratio of 1:10, remove the calcium carbonate, and then wash and collect the insoluble substances, and dry them at 40-60℃ until completely dry, and obtain ganoderma; weigh it on a balance, and the weight is 0.074 g; calculate the mass fraction of the ganoderma component by (0.074 / 5) x 100%, which is 1.5%.

[0036] Example 2 A detection method of ganoderma component in a leather for automobile, the leather for automobile is a PU leather containing ganoderma component, the PU leather contains a base cloth, and since the dense layer is extremely thin, the PU leather cannot add ganoderma ultrafine powder, and the detection method comprises the following steps: Dissolution and separation of the sample to be tested: (1) soak the PU leather in acetone, separate the base cloth from the resin after 30 s, remove the base cloth, and retain the resin layer as the sample to be tested.

[0037] (2) Weigh 3g of the sample to be tested, cut into small pieces less than 5mm, and put into a glass beaker, and cold soak with 80mL of N,N-dimethylformamide at room temperature until the resin is completely dissolved.

[0038] (3) Centrifuge the dissolved sample to be tested at a speed of 5000r / min for 10min, separate the supernatant and the precipitate, and wash the precipitate with N,N-dimethylformamide for 3 times, each time with a dosage of 10mL, and combine the washed liquid to the supernatant.

[0039] (4) Continue to centrifuge the supernatant at a speed of 5000r / min for 10min, and repeat step (3) to treat the supernatant and the precipitate, to obtain the treated supernatant and the precipitate.

[0040] Qualitative detection analysis of the supernatant: Press the potassium bromide into a tablet, then drop 0.2mL of the supernatant prepared in step (4) onto the tablet, and dry completely, and then perform Fourier transform infrared spectrum test, and the results are shown in Figure 3 From Figure 3 , it can be seen that the main spectral peaks indicate that the main component is PU, and the characteristic peaks of ganoderma cellulose are around 1120cm -1 and 1020cm -1 .

[0041] Quantitative detection analysis of the precipitate: (5) Collect the precipitate prepared in step (4), and dry at 40℃ until completely dry, then weigh on a balance, and the weight is 0.008g.

[0042] (6) Dissolve all the insoluble substances prepared in step (5) in 20mL of hydrochloric acid solution with a concentration ratio of 1:10, remove calcium carbonate, and then wash and collect the insoluble substances, and dry at 40-60℃ until completely dry, to obtain ganoderma; weigh on a balance, and the weight is 0.003g, and there is little insoluble substance, indicating that the ganoderma additive has been completely extracted in N,N-dimethylformamide.

[0043] Quantitative detection analysis of the supernatant: Take all the supernatant prepared in step (4), and perform rotary evaporation concentration, and then redissolve with 2mL of chromatographic grade methanol, and inject 1mL into a high performance liquid chromatography sample bottle, and set the running conditions to realize full spectrum scanning; select 4 kinds of ganoderma acid (ganoderma acid A, ganoderma acid B, ganoderma acid D, and ganoderma acid F) as the standard sample to perform liquid phase analysis under the same conditions, and the results are shown in Figure 4As shown, based on the response ratio, the mass proportion of the Ganoderma lucidum component was calculated to be 0.24%. The chromatographic conditions were: a ZORBAX SB-Aq C18 column (4.6 mm × 250 mm, 5 μm), a column temperature of 30°C; mobile phase A: acetonitrile, mobile phase B: 0.1% aqueous phosphoric acid; gradient elution, and a detection wavelength of 252 nm. The gradient elution program is shown in Table 1.

[0044] Table 1:

[0045] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the sample to be tested was not dissolved and separated, and was directly detected by Fourier transform infrared spectroscopy. The results are as follows: Figure 5 As shown. Figure 5 It can be seen that the PVC resin and the Ganoderma PVC resin are highly overlapped, and no obvious independent chromatographic peak of Ganoderma cellulose can be obtained, and the purpose of separation cannot be achieved, that is, no qualitative analysis of Ganoderma can be performed.

[0046] Comparative Example 2 The difference between Comparative Example 2 and Example 2 is that the sample to be tested was not dissolved and separated, and Fourier transform infrared spectroscopy was directly used for detection. The results are as follows: Figure 6 As shown. Figure 6 It can be seen that the PU resin and the Ganoderma PU resin are highly overlapped, and no distinct independent chromatographic peak of Ganoderma cellulose can be obtained, and the purpose of separation cannot be achieved, that is, no qualitative analysis of Ganoderma can be performed.

[0047] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is only that step (6) is different. Step (6) of Comparative Example 3 is as follows: The insoluble matter obtained in step (5) was completely dissolved in 20 mL of hydrochloric acid solution with a concentration ratio of 1:10 to remove calcium carbonate. The insoluble matter after hydrochloric acid treatment was then subjected to thermal decomposition analysis at 550 ° C. The total ion current diagram is shown as follows: Figure 7 As shown. Figure 7 It can be seen that the figure mainly reflects the information of PVC additives. The characteristic mass-to-charge ratios of Ganoderma cellulose are 60 and 98, which do not appear in the figure, indicating that the thermal cracking detection method cannot detect the information of Ganoderma cellulose or Ganoderma powder.

[0048] Comparative Example 4 The difference between Comparative Example 4 and Example 2 is that the mobile phase in the chromatographic conditions of the quantitative detection and analysis step of the supernatant is different. The mobile phase in the chromatographic conditions of Comparative Example 4 is a single 0.1% phosphoric acid aqueous solution. The results are as follows: Figure 8 As shown. Figure 8It can be seen that the effective peak cannot be separated and the ganoderma component cannot be detected by using a single mobile phase of 0.1% phosphoric acid aqueous solution.

[0049] Accuracy and repeatability of detection 1. Standard curve of ganoderma acid According to the detection conditions of the high-performance chromatographic analysis of Example 2, the standard solutions of ganoderma acid with concentrations of 0.5 mg / mL, 1 mg / mL, 2.5 mg / mL, 5 mg / mL, 10 mg / mL and 15 mg / mL were detected and analyzed, and then a standard curve was drawn with the concentration as the horizontal coordinate and the response value as the vertical coordinate, and the results are shown in Table 2. It can be seen from Table 2 that the detection method has good linearity in the range of 0.5-15 mg / mL of the concentration of ganoderma acid, the linear correlation coefficient reaches 0.998, and meets the quantitative calculation requirements.

[0050] Table 2:

[0051] 2. Repeatability test The above-mentioned mixed standard solution of ganoderma acid was prepared into a standard sample tube containing target compounds (ganoderma acid A, ganoderma acid B, ganoderma acid D and ganoderma acid F) with a concentration of 2.00 μg, the intra-day and inter-day repeatability of the method was detected, and the results are shown in Table 3. It can be seen from Table 3 that the intra-day repeatability is in the range of 2.55-4.44%, the inter-day repeatability is in the range of 2.03-5.42%, and the method has good repeatability.

[0052] Table 3:

[0053] 3. Accuracy test The above-mentioned mixed standard solution of ganoderma acid was prepared into a standard sample tube containing target compounds (ganoderma acid A, ganoderma acid B, ganoderma acid D and ganoderma acid F) with a concentration of 2.00 μg, the accuracy of the method was evaluated, and the results are shown in Table 4. It can be seen from Table 4 that the measured value deviation is between 2.88-5.29%, indicating that the method has high accuracy.

[0054] Table 4:

[0055] For those skilled in the art to which the present application belongs, without departing from the concept of the present application, a number of simple deductions or substitutions can be made without having to undergo creative labor. Therefore, the simple improvements made by those skilled in the art to the present application according to the disclosure of the present application should be within the protection scope of the present application. The above-mentioned embodiments are preferred embodiments of the present application, and any similar processes and equivalent changes made should be within the protection scope of the present application.

Claims

1. A method for detecting Ganoderma lucidum components in leather, characterized in that: The following steps are involved: (1) Weigh M1 of the leather resin to be tested, dissolve it in an organic solvent, and centrifuge to obtain a supernatant and a precipitate; The raw materials for preparing the leather resin to be tested include Ganoderma lucidum; (2) performing Fourier transform infrared spectroscopy on the supernatant to determine whether Ganoderma lucidum cellulose is present in the supernatant; When the Ganoderma lucidum is insoluble or slightly soluble in the organic solvent, the precipitate is tested by a weight method; the weight method comprises the following steps: acid washing the precipitate, washing the insoluble matter, and drying to obtain the Ganoderma lucidum; weighing the Ganoderma lucidum, recorded as M2, and calculating the content of the Ganoderma lucidum component in the leather resin to be tested by (M2 / M1)×100%; When the Ganoderma lucidum is soluble in the organic solvent, the supernatant is detected by high performance liquid chromatography; the detection steps of the high performance liquid chromatography include: concentrating and re-dissolving the supernatant, and then performing full spectrum scanning; performing liquid phase analysis with a ganoderic acid standard sample, and calculating the content of the Ganoderma lucidum component in the leather resin to be tested according to the response result ratio.

2. The method for detecting Ganoderma lucidum components in leather according to claim 1, wherein In step (1), the organic solvent is selected from at least one of tetrahydrofuran, N,N-dimethylformamide, dichloroethane, and cyclohexane.

3. The method for detecting Ganoderma lucidum components in leather according to claim 1 or 2, characterized in that, In step (1), the mass volume ratio of the leather resin to be tested to the organic solvent is (2-20) g:100 mL.

4. The method for detecting Ganoderma lucidum components in leather according to claim 1, wherein: In step (1), the process conditions for the centrifugal separation are: centrifugation at a speed of 4000-8000 rpm for 5-10 minutes.

5. The method for detecting Ganoderma lucidum components in leather according to claim 1, wherein: In step (1), the leather resin to be tested includes automotive leather, and the automotive leather is selected from any one of vinyl chloride leather, polyurethane leather, and polyurethane microfiber leather; and / or the Ganoderma lucidum is selected from at least one of Ganoderma lucidum micropowder, Ganoderma lucidum fermentation aqueous treatment liquid, and Ganoderma lucidum fungus culture.

6. The method for detecting Ganoderma lucidum components in leather according to claim 1, characterized in that: In step (2), the pickling is performed using a hydrochloric acid solution with a concentration ratio of (0.5-1.5):10; and / or the drying temperature is 40-60°C.

7. The method for detecting Ganoderma lucidum components in leather according to claim 1, characterized in that: In step (2), the concentration method includes rotary evaporation.

8. The method for detecting Ganoderma lucidum components in leather according to claim 1, characterized in that: In step (2), the re-dissolving solvent includes chromatographic grade methanol.

9. The method for detecting Ganoderma lucidum components in leather according to claim 1, characterized in that: In step (2), the ganoderic acid standard sample includes at least two of ganoderic acid A, ganoderic acid B, ganoderic acid D, and ganoderic acid F.

10. The method for detecting Ganoderma lucidum components in leather according to claim 1, characterized in that: In step (2), the chromatographic conditions of the high performance liquid chromatography method include: the chromatographic column is filled with octadecyl bonded silica gel, the column temperature is 25-35°C; the mobile phase A is acetonitrile, and the mobile phase B is a phosphoric acid aqueous solution; the detection wavelength is 250-260nm, and a gradient elution program is used.

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