Morel GZEF01 and application thereof

Abstract

The application discloses a Morchella elata strain GZEF01, the strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M2025943. The application also discloses the use of the strain in the preparation of health-care products, beverages and the like; and the application also discloses a molecular marker for identifying the strain. The ascocarps of the strain GZEF01 are light brown and mushroom-shaped and long, and have high commercial value. In addition, the strain has a short spawn preparation period, about 29 days, and high spawn production efficiency. The cultivation period of the strain from seeding to mushroom harvesting is only 65-70 days, the cultivation period is short, the labor cost is reduced, the strain can be put on the market before the Spring Festival, and higher sales and economic benefits can be obtained. The strain GZEF01 also has the advantages of high fruit body yield and strong resistance to cadmium stress.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of edible fungi, and particularly relates to a Morchella elata strain and use of the strain. BACKGROUND

[0002] Morchella elata (belonging to Morchella elata species) is rich in nutrition, containing 8 kinds of essential amino acids, vitamins, trace elements and carbohydrates, and polysaccharides, and has immune regulation effect. In addition, Morchella elata has delicious taste and unique flavor. With the improvement of people's living standards, the demand for Morchella elata and other Morchella is increasing, and the existing production of Morchella elata is difficult to meet the demand.

[0003] At present, the main problems existing in the production of Morchella elata are: (1) few strains; (2) long production cycle of strains. At present, the production cycle of Morchella elata from mother strain to cultivation strain is generally 58-68 days, the strain production time is long, and the production efficiency of Morchella elata strain is not high; (3) long cultivation period from seeding to fruiting. The price of fresh Morchella on the market before the Spring Festival is relatively high, which is several times of the price after the Spring Festival. Morchella is generally cultivated in the field at the end of October to early November every year, and the existing Morchella elata strain has a growth period of 105-120 days from seeding to fruiting, and the fruiting time is from early February to late March of the next year, which is difficult to catch up with the fruiting before the Spring Festival, and the profit is relatively low; in addition, the long cultivation period means facing more climate risks, which affects the yield and quality of Morchella elata and economic benefits; (4) low yield. The average yield of the existing Morchella elata variety is only 130-147 kg per mu; (5) the content of heavy metal cadmium is high in many places in southwest China, and generally "with the increase of cadmium ion stress concentration, the growth rate of Morchella mycelium slows down until it does not grow" (Li Lang et al., Jiangsu Agricultural Science, 2024), which limits the development of Morchella in the local area, and screening or breeding of Morchella with cadmium stress resistance is an effective way to solve the problem. At present, there is no report on Morchella elata strain with cadmium stress resistance.

[0004] There are rich wild Morchella resources in the original forest in southwest China, and the Morchella elata strain with short strain production time, short cultivation period from seeding to fruiting, high yield and cadmium stress resistance can be obtained through screening and domestication. SUMMARY

[0005] The present application aims to provide a Morchella elata strain.

[0006] Another object of the present application is to provide the use of the above-mentioned Morchella elata strain.

[0007] A third object of the present application is to provide a molecular marker for identifying the above-mentioned Morchella elata strain.

[0008] To achieve the above object, the technical scheme of the present application is as follows:

[0009] A Morchella elata strain GZEF01 is preserved in China Center for Type Culture Collection, Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2025943, and the preservation date is April 29, 2025.

[0010] The present application also provides the application of the above-mentioned strain GZEF01 in food or health products.

[0011] The present application also provides a food or health product, which contains the above-mentioned strain GZEF01 or the extract of GZEF01.

[0012] The present application also provides the application of the above-mentioned strain GZEF01 in beverages.

[0013] The present application also provides a beverage, which contains the above-mentioned strain GZEF01 or the extract of GZEF01.

[0014] The present application also provides the application of the above-mentioned strain GZEF01 in the preparation of drugs for improving human immunity.

[0015] The present application also provides a molecular marker for identifying the above-mentioned strain GZEF01, which is a DNA molecule fragment; the nucleotide sequence of the DNA molecule fragment is shown in SEQ ID NO: 1.

[0016] Compared with the existing Morchella esculenta strains, the present application has the advantages and beneficial technical effects as follows: (1) The ascocarps of the strain GZEF01 of the present application are light brown and mushroom-shaped and long, and have high commercial value. (2) The preparation period of the strain GZEF01 of the present application is short. The preparation period of the strain GZEF01 from the mother strain to the cultivated strain is only 29 days, which is significantly shorter than the preparation period of other existing Morchella esculenta strains from the mother strain to the cultivated strain, and the production efficiency of the strain is higher. (3) The cultivation period of the strain GZEF01 of the present application is short. The cultivation period of the strain GZEF01 from seeding to harvesting is only 65-70 days, which is significantly shorter than the cultivation period of other existing Morchella esculenta strains from seeding to harvesting. The short cultivation period not only reduces the labor cost, but also facilitates the market before the Spring Festival, and higher fresh mushroom sales price and economic benefits are obtained. In addition, the short cultivation period also reduces the climate risks that may be encountered during cultivation, and accordingly reduces the influence on the yield and quality of Morchella esculenta. (4) The yield of the fruiting body of the strain GZEF01 of the present application is high. The strain GZEF01 is cultivated in a cold shed in southern China in early November, and is harvested in early January of the next year. The strain GZEF01 not only can be marketed before the Spring Festival, but also has an average yield of 712.3 kg / 667m 2 , which is much higher than the yield of 130-147 kg / 667m 2 of other existing Morchella esculenta strains. The single mushroom weight of the strain GZEF01 is 34.5-57.4 g, and the average single mushroom weight is 43.74 g. (5) The strain GZEF01 of the present application has a strong resistance to cadmium stress. Under different cadmium stress concentrations, the growth rate of the solid strain mycelium and the biomass of the liquid strain mycelium are both significantly higher than those of other Morchella esculenta varieties, which indicates that the strain GZEF01 has a significantly higher resistance to cadmium stress than other main cultivated Morchella esculenta varieties, and the planting area of Morchella esculenta is expanded, and the income of mushroom farmers is increased. (6) The strain GZEF01 of the present application has a large proportion of cap in the fruiting body and a high rehydration rate of dry products, and has excellent commercial properties. (7) The strain GZEF01 of the present application has a high content of nutritional components in the fruiting body. In the dry mushroom, the content of crude polysaccharide is 7.58 g / 100 g, the content of crude protein is 46.51 g / 100 g, and the total content of amino acids is 35.56 g / 100 g.

[0017] Biological preservation: The Morchella elata strain GZEF01 of the present application is a wild Morchella elata strain collected by the inventor in Bijie, Guizhou Province in April 2017, and is screened and cultivated. The strain has been preserved in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2025943, and the preservation date is April 29, 2025. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 . The fruiting body photo of Morchella elata GZEF01 of the present application.

[0019] Figure 2 . The phylogenetic tree diagram of Morchella elata GZEF01 of the present application. DETAILED DESCRIPTION

[0020] The present application is further described below through specific examples, but does not constitute any limitation on the present application.

[0021] Example 1: Collection, isolation, identification and breeding process of Morchella elata GZEF01 of the present application

[0022] (1) Strain isolation

[0023] The present inventors accidentally discovered a wild Morchella elata fruiting body in Bijie, Guizhou Province in April 2017. The mycelium (strain) was obtained by tissue isolation of the fruiting body. It was identified as Morchella elata through morphological characteristics, ITS-PCR detection and phylogenetic tree construction, as well as gene fragment size and sequence comparison. The strain isolated by tissue isolation was cultivated to carry out mushroom cultivation test. The fruiting bodies with good bud type were subjected to strain isolation, inoculated on PDA medium for culture, and the mushroom cultivation test was carried out in the experimental base of Guizhou Academy of Agricultural Sciences. After the mushroom cultivation, the ascospores were collected, and a plurality of tetrasomic self-intersection strains were obtained through tetrasomic self-intersection method.

[0024] (2) Strain domestication

[0025] The genomic DNA of the tetrasomic self-intersection strain obtained in step (1) was extracted according to the DNA extraction kit instruction. The mating type genes MAT1-1 and MAT1-2 of the monospore strain were amplified by using two groups of universal primer combinations P8-F / P8-R and P10-F / P10-R (the primers were synthesized by Shengong Bioengineering (Shanghai) Co., Ltd.). The primer sequences are as follows:

[0026] P8-F: 5'-TTACCTTACTGGACTGGTTCGTGAG-3';

[0027] P8-R: 5'-TGGAATGTCTGTGATTGAGGCTGTG-3'.

[0028] P10-F: 5'-GGCCAGAACAGATGCTCGAAGAAGC-3';

[0029] P10-R: 5'-GTGGCAACTCCCAAAGCATGATCAA-3'.

[0030] The reaction system (total volume 20 μL) of the PCR is: 20-50 ng / μL of DNA template 1 μL, 10x Buffer (with Mg2+) 2 μL, 2.5 mM dNTP 0.5 μL, 5 U / μL DNA polymerase 0.2 μL, 0.2 μM primer each 0.5 μL, add double distilled water to 20 μL. The PCR reaction condition is: pre-denaturation 94℃ 5 min; 94℃ 30 s, 63℃ 30 s, 72℃ 3 min, 30 cycles; 72℃ extension 10 min.

[0031] The product after PCR amplification is detected by electrophoresis, and the mating type of the single-spore strain with the MAT1-1 gene band and without the MAT1-2 gene band is MAT1-1. On the contrary, the mating type of the single-spore strain with the MAT1-2 gene band and without the MAT1-1 gene band is MAT1-2. The double-mating-type strain (both the MAT1-1 gene band and the MAT1-2 gene band) is selected, and after several generations of culture, a strain with stable genetic traits, fast mycelial growth, high yield and strong stress resistance is screened out through various trait comparison tests, and is named GZEF01.

[0032] The mating type of the strain GZEF01 of the application is complete, the genetic material is more complete than that of the traditional tissue separation strain, and the strain passage trait is more stable. After multiple generations of cultivation tests, the excellent agronomic traits are stable.

[0033] Example 2: Classification and identification of the strain GZEF01 of the application

[0034] (1) Morphological identification of GZEF01

[0035] The ascocarps of GZEF01 are brown, the fruiting bodies are 10-18 cm high, the caps are 7-13 cm high and 2-5 cm thick, are broad-conical in shape, have a blunt top, and the lower edge links with the stipe, the ascocarps have developed longitudinal ridges and sparse transverse ridges, and are covered with short hairs on the ridges, the transverse and longitudinal ridges form pits, and the bottom of the pits is lighter than the ridges. The stipe is 3-6 cm high and 2-4 cm thick, is ivory white, and is cylindrical and hollow. The ascus is columnar, and each ascus contains eight spores arranged in a single longitudinal row. According to the description of the morphological characteristics of Morchella elata on pages 39 and 45 of “Classification and Ecology of Morchella in Yunnan” (Li Yingxia, Yunnan Agricultural University, 2015), the strain belongs to the Morchella elata species.

[0036] (2) Molecular biological identification of GZEF01

[0037] The genomic DNA of the GZEF01 strain was extracted by using a new plant genomic DNA extraction kit CW0531 (purchased from Beijing Kangwei Century Company) according to the instruction thereof, and PCR amplification was performed by using universal primers ITS1 and ITS4 (the primers were synthesized by Shenguo Bioengineering (Shanghai) Co., Ltd.) as primers. The primer sequences are as follows:

[0038] ITS1: 5'-TCCGTAGGTGAACCTGCGG-3';

[0039] ITS4: 5'-TCCTCCGCTTATTGATATGC-3'.

[0040] The PCR reaction system (total volume 20 μL) was as follows: 20-50 ng / μL of DNA template 1 μL, 10×Buffer (with Mg 2+ ) 2 μL, 2.5 mM dNTP 0.5 μL, 5 U / μl DNA polymerase 0.2 μL, 0.2 μM primer 0.5 μL each, and double-distilled water to 20 μL. The ITS-PCR reaction condition was as follows: pre-denaturation 94℃ for 5 min; 94℃ for 1 min, 60℃ for 1 min, 72℃ for 75 s, 30 cycles; and 72℃ extension for 10 min.

[0041] A DNA molecule fragment (ITS sequence) with a length of 689 bp was obtained by amplification, and the amplification product was sequenced by Shenguo Bioengineering (Shanghai) Co., Ltd. The nucleotide sequence of the DNA molecule fragment is shown as SEQ ID NO: 1.

[0042] The ITS sequence of the obtained GZEF01 strain was subjected to BLAST comparison, and the result showed that the ITS sequence had the highest similarity to Morchella elata, up to 99.71%; and a phylogenetic tree was constructed. From the ITS sequence similarity comparison result and the phylogenetic tree (see Figure 2 ), it can be seen that the strain GZEF01 of the application belongs to Morchella elata, and the GZEF01 is different from other existing Morchella elata strains, and is a new Morchella elata strain.

[0043] Example 3 Cultivation test of the strain GZEF01 of the application

[0044] (I) Test materials

[0045] (1) the strain GZEF01 of the application; (2) Morchella esculenta No. 5 (Guizhou Institute of Soil and Fertilizer); and (3) Morchella esculenta No. 2 (introduced from Sichuan Academy of Agricultural Sciences).

[0046] (II) Test method

[0047] (1) Master culture: the GZEF01 strain (the strain has been preserved in China Center for Type Culture Collection, Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2025943) with a diameter of 5 mm is inoculated in the central part of a master culture medium plate by using a sterilized and cooled inoculation hook, and is cultured at 20℃, an air relative humidity of 60-75%, and in the dark for 5 days. The mycelium grows over the plate, and a large number of sclerotia are formed on the plate at this time, and the GZEF01 master strain is obtained. The master culture medium comprises the following components and their preparation method: 200 g of peeled potatoes are cut into 2 cm squares, and are boiled in 800 mL of RO water for 20 min. The extraction liquid is obtained by filtering through 3 layers of gauze. Then, 20 g of glucose, 20 g of agar, 1 g of potassium dihydrogen phosphate, and 1 g of magnesium sulfate are added, and the mixture is uniformly mixed and diluted with RO water to 1000 mL. The mixture is sterilized at 121℃ for 30 min, and is poured into a 9 cm diameter plate.

[0048] (2) Original culture: the GZEF01 master strain obtained in step (1) is inoculated on an original culture medium at a mass ratio of 1:30 by using a sterilized and cooled strain shovel, and is cultured. The mycelium grows over the flask, and a large number of sclerotia are formed on the flask at this time, and the GZEF01 original strain is obtained. The original culture medium comprises the following components and their preparation method: 85% of wheat, 8% of humus soil, 5% of rice husk, 1% of gypsum, and 1% of calcium carbonate. The culture medium has a water content of 60-65%. After being uniformly stirred, the mixture is filled into a polypropylene plastic bag (16 cm x 35 cm x 0.005 cm), and the opening is tied with a polypropylene rope. The mixture is sterilized at 121℃ for 2 hours.

[0049] (3) Cultivation strain culture: the GZEF01 original strain obtained in step (2) is inoculated on a cultivation strain culture medium at a mass ratio of 1:30 by using a sterilized and cooled strain shovel, and is cultured at 20℃, an air relative humidity of 60-75%, and in the dark for 12 days. The flask forms a large number of sclerotia at this time, and the GZEF01 cultivation strain is obtained. The cultivation strain culture medium comprises the following components and their weight percentage: 85% of wheat, 8% of humus soil, 5% of rice husk, 1% of gypsum, and 1% of calcium carbonate. The culture medium has a water content of 60-65%. After being uniformly stirred, the mixture is filled into a polypropylene plastic bag (16 cm x 35 cm x 0.005 cm), and the opening is tied with a polypropylene rope. The mixture is sterilized at 121℃ for 2 hours.

[0050] (4) Ridge sowing and film mulching: a field with a pH of 6-6.5 and fine soil is selected, and the soil is plowed after removing weeds. The soil is plowed before sowing, and the ridge surface is 80-100 cm wide. A 50 cm wide path is left between the ridges. The soil has a water content of 40-45%. The GZEF01 cultivation strain obtained in step (3) is broken into small particles, and is uniformly sown on the ridge surface. 500 bags of cultivation strain are sown per mu of cultivated land. A thin layer of soil is immediately and uniformly covered after sowing, and a black polypropylene film is covered after covering the soil.

[0051] (5) Mycelium culture and placement of nutrient bags: After sowing, the temperature in the shed is controlled at 15-20 DEG C, the air relative humidity is 60-70%, the soil water content is 40-45%, and the light intensity is 50-100 lux. After 5 days of mycelium growth, the white "frost" is formed on the surface of the plot, and the nutrient bags are placed. Each bag is punched with 40-60 small holes, and the punched surface is tightly attached to the soil surface. 1800 bags of nutrient bags are evenly placed per mu of land, and the black polypropylene film is covered again after the placement of the nutrient bags. The nutrient bags are removed after 40 days of placement in the field, and the black polypropylene film and the nutrient bags are removed. The preparation method of the nutrient bag: 92% of wheat, 7% of cotton seed hulls and 1% of quicklime are uniformly mixed according to the weight percentage, and the water content is 60-65%. The mixed nutrient material is loaded into the bag, the polypropylene rope is tied, and the bag is sterilized at 121 DEG C for 1 hour, and then cooled to room temperature for standby.

[0052] (6) Primordium differentiation: After the removal of the nutrient bags in step (5), the soil water content is sprayed to 45-55%, the temperature in the shed is controlled at 10-20 DEG C, the air relative humidity is 75-85%, and the light intensity is 200-300 lux. The primordium is induced to form, and a large number of primordia are formed 5 days after spraying.

[0053] (7) Mushroom management and harvesting: The temperature in the shed is controlled at 10-20 DEG C, the air relative humidity is 70-80%, the soil water content is 40-45%, the light intensity is 500-1000 lux, and the carbon dioxide concentration is 500-700 ppm. The high temperature is used for spraying, side window ventilation and other measures to reduce the temperature. From the primordium to the mature of the fruiting body, it takes about 15-20 days. When the fruiting body no longer increases, the ridge and the pit of the cap are clear, and the ascus fruit is basically unfolded, which is mature, and should be harvested.

[0054] The observation results show that the ascus fruit of the strain GZEF01 (see Figure 1 ) is brown, the fruiting body is 10-18 cm high, the cap is 7-13 cm high, the cap accounts for more than 70% of the total height, and the commodity value is high. In addition, the single mushroom weight of GZEF01 is 34.5-57.4 g, and the average single mushroom weight is 43.74 g, and the single mushroom weight is high.

[0055] From the above steps (1) to (3), it can be seen that the preparation time of the mother strain of the strain GZEF01 is 5 days, and the preparation of the original strain from the mother strain takes 12 days, and the preparation of the cultivation strain from the original strain takes 12 days, that is, the total cycle of the preparation of the cultivation strain from the mother strain is 29 days. The preparation cycle of the mother strain to the cultivation strain of other Morchella esculenta is 58-68 days, so the preparation time of the strain GZEF01 is much shorter than that of other Morchella esculenta, and the production efficiency of the strain is higher.

[0056] As can be seen from the above steps (4) to (7), the cultivation period of the strain GZEF01 of the present application is short, and the cultivation period from sowing to harvesting is 65-70 days, while the cultivation period of other existing Morchella esculenta strains is 105-120 days. The short cultivation period not only reduces the labor cost, but also facilitates early mushroom marketing, and obtains higher sales price and economic benefit. According to the industry rule of Morchella, as mentioned in the article “Current Situation and Development Thinking of Morchella Industry in Guizhou Province” published by Wang Qin in the journal “Edible Fungi” in 2022: “At present, Morchella is mainly marketed from late January to mid-March, and the price of fresh products ranges from 260 yuan / kg to 110 yuan / kg, with large price fluctuations”. It can be seen that the price of Morchella marketed in January is much higher than that of Morchella marketed in February and March, and the price of Morchella marketed in February and March will drop significantly. The price of Morchella marketed in the early stage is 2-3 times higher than that of Morchella marketed in large quantities in February and March. Therefore, the cultivation period of the high Morchella strain GZEF01 of the present application from sowing to harvesting is short, which is a more suitable early mushroom high Morchella, and improves the sales price of fresh mushrooms and has higher economic value. In addition, the short cultivation period also reduces the climate risks that may occur during cultivation, and accordingly reduces the impact on the yield and quality of high Morchella.

[0057] As can be seen from Table 1, the strain GZEF01 of the present application is cultivated in a cold shed in southern China in early November, and is harvested in early January of the next year, which is conducive to the marketing of fresh mushrooms before the Spring Festival and obtaining a higher sales price of fresh mushrooms. In addition, the average yield of the fruiting body of GZEF01 is 712.3 kg / 667m 2 , which is much higher than the yield of 130-147 kg / 667m 2 of other existing high Morchella.

[0058] Table 1 Comparison of cultivation period and yield of GZEF01 of the present application and other high Morchella

[0059] GZEF01 Quebec Morel No. 5 Sichuan Morel No. 2 Cultivation period (d) 65～70 100～110 110～130 Yield (kg / 667m 2 )]]> 712.3 147 130

[0060] Example 4 Test of nutritional components of high Morchella GZEF01 of the present application

[0061] (I) Test material: GZEF01 fruiting bodies harvested in Example 3.

[0062] (II) Test method:

[0063] (1) After hot air drying of the harvested fruiting bodies, the nutritional components in the fruiting bodies are determined.

[0064] (2) The crude polysaccharide content is determined according to the method in NY / T 1676-2023 "Determination of Crude Polysaccharide in Edible Fungi"; the protein content is determined according to the method in GB5009.5-2016 "National Food Safety Standard-Determination of Protein in Food"; and the total amino acid content is determined according to the method in GB5009.124-2016 "National Food Safety Standard-Determination of Amino Acids in Food".

[0065] Results In the dried mushroom of the strain GZEF01 of the application, the crude polysaccharide content is 7.58 g / 100 g, the crude protein content is 46.51 g / 100 g, and the total amino acid content is 35.56 g / 100 g. It is shown that the nutritional value of the fruiting body of the strain GZEF01 of the application is high.

[0066] Example 5 Heavy metal cadmium stress test of the solid spawn of the strain GZEF01 of the application

[0067] (I) Test materials:

[0068] (1) The strain GZEF01 of the application.

[0069] (2) Qianyangduofungus No. 5 (from Guizhou Institute of Soil and Fertilizer)

[0070] (II) Test method:

[0071] (1) Mycelial growth of the solid spawn under heavy metal cadmium stress: different concentrations of cadmium standard solution are added to the PDA medium, and the final concentration of cadmium in the medium is 2 mg / L, 5 mg / L and 10 mg / L, respectively. The PDA medium without adding the standard solution is used as a blank control (CK), and there are 8 treatments in total. The pH of all the media is adjusted to 7. Different concentrations are set in 3 groups of repeats, and the blocks are inoculated and cultured in a 23℃ incubator. The mycelial growth in the medium with different concentrations of heavy metals is recorded.

[0072] (2) Mycelial growth rate determination: after the blocks are inoculated in the solid medium, the culture is stopped when any one treatment covers the plate, the growth time is recorded, the plate is photographed, the colony diameter of each treatment is measured by cross-line method, and the growth rate is calculated.

[0073] Results (see Table 2) The mycelial growth rate of the strain GZEF01 of the application under different concentrations of cadmium stress is obviously higher than that of other Morchella esculenta. It is shown that the heavy metal cadmium stress resistance of the strain GZEF01 of the application is obviously higher than that of other main cultivars, which expands the cultivation range of Morchella esculenta and is beneficial to improve the income of mushroom farmers.

[0074] Table 2 Comparison test results of the heavy metal cadmium stress resistance of the solid spawn of the strain GZEF01 of the application (mycelial growth rate (mm / d))

[0075]

[0076]

[0077] Example 6 Heavy metal cadmium stress test of GZEF01 liquid spawn of the application

[0078] (I) Test materials:

[0079] (1) The strain GZEF01 of the application.

[0080] (2) No. 5 of Morel (from Guizhou Institute of Soil and Fertilizer).

[0081] (II) Test method:

[0082] (1) Heavy metal cadmium stress of liquid spawn: different concentrations of cadmium standard solution were added to PDB liquid medium, and the final concentration of cadmium in the medium was 2, 5, and 10 mg / L, respectively, with no standard solution as blank control (CK), and the pH of all media was adjusted to 7. Each medium was inoculated with Morel strain for culture, 6 pieces of 5 mm diameter mycelial blocks were inoculated, and the culture was incubated in a 23℃ shaking bed at 150 r / min in the dark for 5 days, and 3 replicates were set for each treatment. The mycelial growth in different concentrations of heavy metal medium was recorded.

[0083] (2) Mycelial biomass determination after heavy metal cadmium stress of liquid spawn: the mycelial balls in the liquid medium were filtered with a 100 mesh filter, and the mycelial balls were repeatedly washed with RO water. Then the mycelium was placed in an oven at 45℃ and dried to constant weight, and the dry mycelium weight was measured.

[0084] Results (see Table 3) The mycelial biomass of Morchella esculenta GZEF01 of the application under different concentrations of cadmium stress was significantly higher than that of other Morchella esculenta. It showed that its heavy metal cadmium stress resistance was significantly higher than that of other main cultivars. It expanded the cultivation range of Morchella esculenta, which was beneficial to improve the income of mushroom farmers.

[0085] Table 3 Comparison test results of strain GZEF01 of the application on heavy metal cadmium stress resistance (mycelial biomass (g / L))

[0086] Cadmium stress concentration (mg / L) GZEF01 (g / L) Quebec Morel No. 5 (g / L) 0 18.99 6.16 2 12.4 1.66 5 7.46 1.43 10 2.91 0.46

Claims

1. A type of morel mushroom ( Morchella elata The strain GZEF01 is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO:M2025943.

2. Use of the strain GZEF01 according to claim 1 in food or health products.

3. Use of the strain GZEF01 according to claim 1 in beverages.

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