Adjustable fluorescence microscope

Abstract

The invention relates to the technical field of microscopes, and discloses an adjustable fluorescence microscope, which comprises a base, a microscope arm arranged at the top of the base, and an object carrying platform arranged on one side of the microscope arm, a lifting device is arranged on the top surface of the base, and one end of the object carrying platform is movably connected with the lifting device; according to the fluorescence microscope, the object carrying platform is controlled by the lifting device to move downwards, and the first optical filter automatically turns over around the fixed shaft to the outer side of the object carrying platform under the action of gravity, so that a horizontal operation space is automatically vacated for the object carrying platform, and after sample replacement is completed, only the object carrying platform needs to be lifted to a preset observation position; when the sample is replaced, only the object carrying platform needs to be controlled to move, repeated manual operation of the optical filter is not needed, so that a worker can conveniently replace the sample in a high-frequency sample replacement scene, the labor time cost of sample detection is further reduced, and meanwhile, the overall working efficiency and operation convenience of the microscopic observation system are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microscopes, and in particular to an adjustable fluorescence microscope. Background Art

[0002] Fluorescence microscopy is a special optical microscope that uses fluorescence phenomena to observe and study the structure and properties of biological samples or materials by detecting fluorescence signals. It can provide highly sensitive and specific imaging results, providing strong support for scientific research and practical applications. It is widely used in biomedicine, materials science, environmental science and other fields.

[0003] Chinese patent publication number CN110320655A provides an adjustable microscope light-shielding structure, which includes a fixed structure, a filter structure and an adjustment structure for adjusting the position of the filter structure. The fixed structure is arranged on a receiving plate between the microscope eyepiece and the objective lens. The fixed structure is connected to the microscope assembly, the fixed structure is connected to the filter structure assembly, and the filter structure is connected to the adjustment structure assembly. During its use, the motor structure drives the semicircular gear to rotate, thereby driving the filter to rotate, so that the filter is opened a certain distance to the outside of the microscope, thereby facilitating the reader to adjust the slide and other operations.

[0004] The existing technology has the problem of operational redundancy. Specifically, when the operator replaces the sample, he must first control the stage to lower vertically to free up vertical working space (i.e., move the stage downward), and then drive the filter to open horizontally to the outside of the microscope to expand the horizontal operating space. After the sample replacement is completed, a reverse reset operation must be performed to control the stage to rise vertically to the preset observation position, and then the filter assembly must be moved back and reset. This repeated manual operation of the filter not only reduces the observation efficiency in high-frequency sample replacement scenarios, but also forces the operator to perform two independent operations during a single sample replacement process (i.e., two operations to drive the filter). When faced with the need for batch sample testing, the accumulated manpower and time costs will increase exponentially, restricting the overall work efficiency of the microscopic observation system. Summary of the Invention

[0005] To achieve the above objectives, the present invention provides an adjustable fluorescence microscope, comprising a base, an arm disposed on the top of the base, and a loading platform disposed on one side of the arm, wherein a lifting device is provided on the top surface of the base, and one end of the loading platform is movably connected to the lifting device;

[0006] A fixed shaft is provided on one side of the mirror arm, and the fixed shaft passes through both sides of the carrier platform. A first filter is rotatably provided on the fixed shaft, and limiting columns are fixed on both sides of the carrier platform to limit the first filter on both sides of the carrier platform; when the lifting device controls the carrier platform to move upward, the first filter is blocked by the limiting columns to flip upward around the fixed shaft to form a filtering area; when the carrier platform moves downward, the first filter automatically flips downward around the fixed shaft under the action of gravity.

[0007] Furthermore, a through slot is formed between the limiting post and the loading platform, and the first optical filter is inserted into the through slot.

[0008] Furthermore, a fixing device is fixedly provided at one end of the fixing shaft away from the mirror arm, and the fixing device is used to limit and fix the first filter.

[0009] Furthermore, both sides of the bottom of the loading platform are provided with slots corresponding to the fixed shaft.

[0010] Furthermore, a control knob is provided on one side of the bottom of the loading platform, and the lifting device includes a servo motor arranged on the base, a steering gear group is provided on one side of the servo motor, and a transmission screw is engaged at one end of the steering gear group, and the transmission screw is rotatably connected to the loading platform, and a screw top plate is provided on the top of the transmission screw, and the screw top plate is fixedly connected to the mirror arm.

[0011] Furthermore, the fixing device includes a positioning baffle fixed to one end of the fixed shaft, a ratchet column is movably provided inside the positioning baffle, a pawl is fixed at one end of the ratchet column, a torsion spring is provided on the outside of the ratchet column, and a one-way ratchet corresponding to the pawl is provided on the rotating sleeve of the fixed shaft away from the end of the mirror arm, a limiting column is fixed on one side of the one-way ratchet, the limiting column is used to press against the outside of the first filter to limit and fix the first filter, and the limiting column and the limiting column are staggered with each other.

[0012] Furthermore, the limiting clamping column includes a connecting block fixedly connected to the one-way ratchet, and a limiting rod is fixed to the end of the connecting block away from the one-way ratchet, and the limiting rod extends to the outside of the first filter.

[0013] Furthermore, a positioning device is fixedly provided on one side of the mirror arm, and a second filter is movably provided on one side of the positioning device. The bottom of the second filter is in contact with the loading platform. When the loading platform moves up, the second filter follows the loading platform and is surrounded by the first filter to form a filtering area.

[0014] Furthermore, the positioning device includes a fixing plate fixedly arranged on one side of the mirror arm, a side positioning plate is fixedly provided on the top of the fixing plate, a supporting base column is provided on both sides of the side positioning plate on the top of the fixing plate, and a baffle assembly is fixed on both sides of the side positioning plate.

[0015] Furthermore, the second filter includes a filter main board movably arranged on one side of the side positioning plate, and filter side plates are fixedly provided on both sides of the filter main board away from the side positioning plate. A movable groove is provided on the side of the filter main board close to the side positioning plate, and the movable groove is adapted to the side positioning plate. A limit baffle is fixedly provided on the side of the filter main board close to the side positioning plate, and the limit baffle, the supporting base column, and the baffle assembly correspond to each other.

[0016] Compared with the prior art, the present invention provides a fluorescence microscope with the following beneficial effects:

[0017] 1. The lifting device controls the downward movement of the loading platform, and the first filter automatically flips around the fixed axis toward the outside of the loading platform under the action of gravity, thereby automatically freeing up horizontal operating space for the loading platform. After completing the sample replacement, it is only necessary to lift the loading platform to the preset observation position. The solution disclosed in the present invention only requires controlling the movement of the loading platform when replacing the sample, and there is no need to repeatedly manually operate the filter, which makes it convenient for staff to replace samples in high-frequency sample replacement scenarios, thereby shortening the manpower and time cost of sample detection, and at the same time improving the overall work efficiency and operational convenience of the microscopic observation system.

[0018] 2. When fine-tuning the stage (for example, when adjusting the focus, fine-tuning is performed downward), the first filter can be fixed in place by a fixture to prevent it from tilting downward under the action of gravity. During downward fine-tuning, the first filter remains stationary, preventing any impact on the filtering effect of the filter area. When the first and second filters form a three-sided filtering area, the fixture can also prevent the formation of a gap between the first and second filters, further ensuring the filtering effect of the filter area. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort.

[0020] Figure 1 This is a schematic diagram of the overall structure of an adjustable fluorescence microscope of the present invention;

[0021] Figure 2 This is a schematic structural diagram of a standby state of an adjustable fluorescence microscope of the present invention;

[0022] Figure 3 This is a schematic diagram of the back structure of an adjustable fluorescence microscope of the present invention;

[0023] Figure 4 Schematic diagram of the second filter structure of the present invention;

[0024] Figure 5 It is a structural schematic diagram of the lifting device of the present invention;

[0025] Figure 6 Schematic diagram of the structure of the loading platform of the present invention;

[0026] Figure 7 Schematic diagram of the first filter structure of the present invention;

[0027] Figure 8 For the present invention Figure 7 A schematic diagram of the structure at center A;

[0028] Figure 9 For the present invention Figure 8 A magnified schematic diagram of the structure at point B in the middle;

[0029] Figure 10 For the present invention Figure 7 Enlarged schematic diagram of the structure at point C in the middle.

[0030] Reference numerals:

[0031] 11. Base; 12. Light source device; 13. Focusing knob; 14. Mirror arm; 15. Wide-angle eyepiece; 16. Charge coupled device; 17. Achromatic objective lens; 22. Loading platform; 23. Specimen clamp; 231. Fixing clamp; 232. Specimen slot; 233. Rotating clamp; 234. Rotating handle; 25. Lifting device; 251. Servo motor; 252. Steering gear set; 253. Transmission screw; 254. Screw top plate; 255. Control button; 26. Slot; 27. Limiting column; 28. Control knob; 31. Fixed shaft; 32. Lower filter plate; 33. First filter; 331. Filter body; 332. Support column; 333. Remove the cone; 334. Connect Connecting column; 335, inserting cone; 336, arc-shaped clamping strip; 34, limiting block; 35, connecting device; 351, connecting ring; 352, docking block; 353, elastic column; 354, triangular clamping column; 36, connecting fixed plate; 37, fixing device; 371, positioning partition; 372, ratchet clamping column; 373, torsion spring; 374, pawl; 375, protective partition; 376, one-way ratchet; 377, limiting clamping column; 41, second filter; 411, filter main board; 412, filter side plate; 413, movable groove; 414, limiting baffle; 42, positioning device; 421, fixing plate; 422, side positioning plate; 423, supporting base column; 424, baffle assembly. DETAILED DESCRIPTION

[0032] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0033] The present invention will be further described below with reference to the embodiments.

[0034] Example 1

[0035] Reference Figure 1 , which is the first embodiment of the present invention, provides an adjustable fluorescence microscope, including a base 11, a mirror arm 14 fixedly arranged on the top of the base 11, and a loading platform 22 arranged on one side of the mirror arm 14; a fixed shaft 31 is provided on one side of the mirror arm 14, and the fixed shaft 31 passes through both sides of the loading platform 22, and a first filter 33 is rotatably provided on the fixed shaft 31, and a lifting device 25 is provided on the top surface of the base 11, and the lifting device 25 is movably connected to the loading platform 22 near the mirror arm 14; the lifting device 25 is used to drive the loading platform 22 to move up and down, so as to facilitate the staff to replace or observe the sample.

[0036] Reference Figure 6 The two sides of the loading platform 22 are fixed with limiting posts 27 for limiting the first filter 33 on both sides of the loading platform 22. A through groove is formed between the limiting posts 27 and the loading platform 22, and the first filter 33 is inserted into the through groove (such as Figure 1 As shown), the purpose of forming the through groove is to facilitate the first filter 33 to move in the direction of movement of the object platform 22 and to avoid direct contact between the first filter 33 and the object platform 22, which would cause wear of the first filter 33.

[0037] Continue reading Figure 6 As shown, both sides of the bottom of the loading platform 22 are provided with card slots 26 corresponding to the fixed axis 31. When the loading platform 22 moves downward, the card slots 26 and the fixed axis 31 gradually approach and overlap with each other. This is the maximum range of the descent of the loading platform 22, thereby preventing the first filter 33 flipped downward from blocking the light source device 12, making it convenient for the staff to adjust or replace the light source device 12 according to actual needs.

[0038] Reference Figure 7 A fixing device 37 is fixed to one end of the fixed shaft 31 away from the mirror arm 14. The fixing device 37 is used to keep the first filter 33 stationary when the object platform 22 is fine-tuned downward, that is, the first filter 33 is in a vertical state, thereby ensuring the filtering effect (see the detailed description below).

[0039] A positioning device 42 (such as Figure 5 As shown), a second filter 41 is movably provided on one side of the positioning device 42, and the bottom of the second filter 41 is in contact with the loading platform 22. When the loading platform 22 moves up, the second filter 41 moves up with the loading platform 22 and forms a filtering area together with the first filter 33.

[0040] When the lifting device 25 controls the upward movement of the loading platform 22, it drives the second filter 41 upward. The limiting posts 27 on both sides of the loading platform 22 squeeze the outer side of the first filter 33, causing it to flip upward around the fixed axis 31 and filter light together with the second filter 41. When the loading platform 22 moves downward, the first filter 33 automatically flips downward around the fixed axis 31 under the action of gravity. When the loading platform 22 is fine-tuned (for example, when adjusting the focus, the fine-tuning is downward), the first filter 33 is restrained and fixed by the fixing device 37 to prevent the first filter 33 from flipping downward under the action of gravity. During downward fine-tuning, the first filter 33 remains stationary to avoid affecting the filtering effect of the filtering area. When the first filter 33 and the second filter 41 cooperate to form a three-sided filtering area, the fixing device 37 restrains and fixes the first filter 33, and also prevents the formation of a gap between the first filter 33 and the second filter 41, further ensuring the filtering effect of the filtering area.

[0041] Compared with the prior art, in this embodiment, during the sample replacement process, the sample carrier 22 is controlled to move downward, and the first filter 33 automatically flips around the fixed axis 31 to the outside of the sample carrier 22 under the action of gravity (such as Figure 2 As shown), the horizontal operation space is automatically freed up for the sample loading platform 22. After the sample replacement is completed, the sample loading platform 22 only needs to be lifted to the preset observation position. That is, during the upward movement of the sample loading platform 22, the second filter 41 and the first filter 33 automatically form a filtering area on the three sides of the sample (as shown). Figure 1 As shown in the figure, it further effectively blocks external light and stray light inside the microscope, improving the signal-to-noise ratio of the image and making observation more accurate.

[0042] When changing samples, it is only necessary to control the movement of the loading platform 22, without the need for repeated manual operation of the filter. This makes it easier for staff to replace samples in high-frequency sample replacement scenarios, thereby reducing the manpower and time costs of sample testing, while improving the overall work efficiency and operational convenience of the microscopic observation system. The specific principle is described as follows:

[0043] Specifically, first, the lifting device 25 controls the loading platform 22 to move freely up and down. During the downward movement of the loading platform 22, since the fixed shaft 31 is fixedly installed on one side of the mirror arm 14, and the first filter 33 is rotatably connected to the surface of the fixed shaft 31, the first filter 33 rotates along the surface of the fixed shaft 31 under the action of gravity, and follows the moving direction of the loading platform 22 to flip downward toward the outside of the loading platform 22 until the loading platform 22 is at the lowest point set on the lifting device 25. At this time, the angle between the first filter 33 and the vertical plane is between 5° and 10° (such as Figure 2 As shown); then the staff places the slide on the loading platform 22 (the sample is placed on the slide) and fixes it. After the slide is placed, the lifting device 25 is started again to control the loading platform 22 to move upward. At this time, the loading platform 22 moves upward along the lifting device 25, and then the limiting column 27 on the loading platform 22 squeezes the outer side of the first filter 33, causing the first filter 33 to flip upward along the fixed axis 31 until the inner side of the first filter 33 is attached to the outer side of the second filter 41. At the same time, the second filter 41 can also play a role of lateral support for the first filter 33, preventing the first filter 33 from contacting and rubbing with the loading platform 22, which affects the filtering effect of the first filter 33.

[0044] The loading platform 22 continues to move upward. During this process, the loading platform 22 first contacts the bottom of the second filter 41, and drives the second filter 41 to move upward along with the loading platform 22 until the loading platform 22 reaches the set observation position. At this time, the first filter 33 and the second filter 41 block and filter the surrounding areas of the loading slide on the loading platform 22, thereby effectively blocking external light and stray light inside the microscope, reducing interference from nonspecific fluorescence, improving the signal-to-noise ratio of the image, and making observation more accurate.

[0045] Example 2

[0046] Reference Figure 8 and Figure 9 As shown in the figure, a second embodiment of the present invention provides an adjustable fluorescence microscope, wherein the first filter 33 includes a filter body 331, a support column 332 is fixedly provided at the bottom of the filter body 331, a connecting column 334 is fixedly provided at the bottom of the supporting column 332, a removal cone 333 is movably provided on the outer side of the connecting column 334, and an insertion cone 335 is fixedly provided on the top of the connecting column 334. The bottom of the filter body 331 is provided with an arc-shaped clamping strip 336 located on one side of the supporting column 332; the outer side of the fixed shaft 31 is movably provided with a take-out cone 333. A limit block 34 is provided, and the arc surface of the arc-shaped card strip 336 matches the protrusion on the limit block 34. The limit block 34 prevents the filter body 331 from continuing to move downward due to its own gravity to be inserted into the connecting device 35 when the filter area is formed with the filter side plate 412, causing the filter body 331 to loosen from the connecting device 35. Then, during the downward movement of the loading platform 22, the filter body 331 follows it to flip downward, loosens from the connecting device 35 and falls, causing the filter body 331 to fall and be damaged.

[0047] The outer side of the fixed shaft 31 is provided with a connecting device 35 located on one side of the limit block 34, and the outer side of the fixed shaft 31 is fixed with a connecting plate 36 ( Figure 4 As shown), the connecting plate 36 is fixedly connected to the mirror arm 14, and a lower filter plate 32 is fixedly provided on the outer side of the fixed shaft 31. The lower filter plate 32 is used to block and filter the lower side of the loading platform 22, thereby further improving the filtering effect during the detection process;

[0048] The connecting device 35 includes a connecting ring 351, which is rotatably sleeved on the surface of the fixed shaft 31. A docking block 352 is fixedly arranged on the top of the connecting ring 351, and a docking groove is opened inside the docking block 352. Elastic columns 353 are fixed on both sides of the docking groove. A triangular clamping column 354 is fixed on one end of the elastic column 353, and the inclined surface of the triangular clamping column 354 faces upward. Only when the inclined surface of the triangular clamping column 354 faces upward can the filter body 331 be quickly disassembled and assembled with the fixed shaft 31, so that the staff can replace the filter body 331 of different colors according to the type of sample and light source. The specific principle is described below in this embodiment.

[0049] See Figure 7 and Figure 10 The fixing device 37 includes a positioning partition 371 fixed to the end of the fixed shaft 31 away from the mirror arm 14, and a ratchet column 372 is rotatably provided inside the positioning partition 371, and a pawl 374 is fixed at one end of the ratchet column 372. A torsion spring 373 is provided on the outside of the ratchet column 372, and the torsion spring 373 is used to ensure that the pawl 374 is always engaged with the one-way ratchet 376. The end of the fixed shaft 31 away from the mirror arm 14 is rotatably sleeved with a one-way ratchet 376 corresponding to the pawl 374. The one-way ratchet 376 can be connected to the fixed shaft 31 through a bearing, and a limiting column 377 is fixed on one side of the one-way ratchet 376. The limiting column 377 is used to press against the outside of the first filter 33 to limit and fix the first filter 33.

[0050] The limiting clamping column 377 includes a connecting block fixedly connected to the one-way ratchet 376 . A limiting rod is fixed to the end of the connecting block away from the one-way ratchet 376 . The limiting rod extends to the outside of the first filter 33 .

[0051] In order to protect the filter body 331 , a protective spacer 375 is provided between the one-way ratchet 376 and the filter body 331 . The protective spacer 375 is fixedly sleeved on the surface of the fixed shaft 31 and does not interfere with the movement of the limiting rod.

[0052] At the same time, the limit posts 377 and the limit posts 27 are staggered with each other to prevent the limit posts 377 from colliding with the limit posts 27 when the loading platform 22 moves down to the lowest point, causing the loading platform 22 to be unable to move down normally.

[0053] Specifically, before using the fluorescence microscope, according to the sample to be detected, select the light source device 12 corresponding to the sample, and then select the filter body 331 and the second filter 41 of the type that is compatible with the light source device 12. Then, move the limit block 34 along the outer side of the fixed shaft 31 toward the side of the connecting ring 351, so that the limit block 34 and the connecting ring 351 are embedded together. At this time, hold the limit block 34 and the connecting ring 351 together (as shown in FIG. Figure 8Then align the insertion cone 335 at the bottom of the filter body 331 with the docking groove of the docking block 352 on the connecting ring 351 until the insertion cone 335 is completely inserted into the docking groove in the docking block 352. At this time, the elastic columns 353 on both sides of the docking groove will limit the insertion cone 335 (as shown in FIG. Figure 9 As shown), at this time, the arc-shaped clamping strip 336 will fit together with the top of the limit block 34, thereby limiting the depth of the insertion cone 335 into the docking block 352. Similarly, another set of insertion cones 335 are plugged into the connecting device 35, thereby removably fixing the filter body 331 to the outside of the fixed shaft 31.

[0054] When the filter body 331 needs to be replaced, first separate the limit block 34 from the connecting ring 351, and move the limit block 34 along the surface of the fixed shaft 31 out of the range of the arc-shaped clamping strip 336, and then the limit block 34 naturally flips downward along the fixed shaft 31 under the action of gravity, and then hold the connecting ring 351 to make the filter body 331 continue to move toward the connecting ring 351 (at this time, the limit block 34 is separated from the arc-shaped clamping strip 336, that is, the limit block 34 leaves the bottom of the arc-shaped clamping strip 336, and there is no interference from the limit block 34, so the filter body 331 can continue to be pressed down), and then insert the cone 335 to continue to penetrate into the docking groove, and remove the cone 333. The insertion cone 335 moves together until the insertion cone 335 moves to the bottom of the docking groove. At this time, the filter body 331 continues to move downward, so that the removal cone 333 continues to move downward along the connecting column 334 until the bottom of the removal cone 333 is in contact with the top of the insertion cone 335. At the same time, the triangular clamping columns 354 at one end of the elastic column 353 are just located on both sides of the removal cone 333. Then, the filter body 331 is pulled outward. Since the removal cone 333 is not fully inserted into the docking groove, the removal of the cone 333 will drive the insertion cone 335 to pass through the two sets of triangular clamping columns 354, so that the insertion cone 335 is removed from the docking block 352.

[0055] At the same time, when the cone 333 is taken out and leaves the clamping of the triangular clamping column 354, the spring inside the cone 333 will automatically reset the cone 333, making it convenient for the staff to reinsert the filter body 331 into the docking block 352 next time, or to replace the type of filter body 331 at any time according to the type of light source, thereby increasing the applicability of the device.

[0056] When the loading platform 22 is fine-tuned up and down, the filter body 331 will automatically flip downward around the fixed axis 31 under the action of gravity when the loading platform 22 moves down, so that a gap is generated between the filter body 331 and the second filter 41. At this time, the staff holds the outer side of the limit post 377 and rotates the limit post 377 counterclockwise around the fixed axis 31, and then the limit post 377 drives the one-way ratchet 376 to rotate until the limit post 377 is attached to one side of the filter body 331. At this time, the limit post 377 is released, and the filter body 331 is blocked by the limit post 377 in the counterclockwise direction; under the restriction of the pawl 374, the one-way ratchet 376 can only rotate counterclockwise and cannot rotate clockwise.

[0057] After the fine adjustment of the loading platform 22 is completed, the ratchet clamp 372 is rotated to separate the pawl 374 at one end of the ratchet clamp 372 from the one-way ratchet 376, and then the limit clamp 377 is rotated clockwise to reset. The limit clamp 377 is separated from the filter body 331, and the restriction on the filter body 331 to flip downward is released (such as Figure 7 As shown); when the object-carrying platform 22 is fine-tuned downward, the first filter 33 is kept stationary, that is, the first filter 33 is in a vertical state to ensure the filtering effect.

[0058] The ratchet clamping column 372 is then released, and the torsion spring 373 outside the ratchet clamping column 372 resets it, and causes the pawl 374 to re-engage the one-way ratchet 376 to limit and fix it.

[0059] Example 3

[0060] Reference Figure 3-Figure 5 , which is a third embodiment of the present invention, provides an adjustable fluorescence microscope, wherein a second filter 41 is movably provided on one side of a positioning device 42, and the positioning device 42 comprises a fixing plate 421 fixedly provided on one side of the mirror arm 14, a side positioning plate 422 is fixedly provided on the top of the fixing plate 421, and a fixing shaft 31 is fixed to one side of the fixing plate 421 (such as Figure 4 As shown in FIG, the top of the fixing plate 421 is provided with supporting base columns 423 located on both sides of the side positioning plates 422, and baffle assemblies 424 are fixedly provided on both sides of the side positioning plates 422. The supporting base columns 423 and the baffle assemblies 424 are used in combination to limit the maximum range of up and down movement of the filter main board 411, thereby preventing the filter main board 411 from interfering with the staff when changing the sample on the loading platform 22.

[0061] Reference Figure 3As shown, the second filter 41 includes a filter main plate 411 movably mounted on one side of a side positioning plate 422. Filter side plates 412 are fixedly mounted on both sides of the filter main plate 411 away from the side positioning plate 422. A movable slot 413 is defined on the side of the filter main plate 411 near the side positioning plate 422, and the movable slot 413 is adapted to fit within the side positioning plate 422. A position-limiting baffle 414 is fixedly mounted on the side of the filter main plate 411 near the side positioning plate 422. The position-limiting baffle 414, the supporting base 423, and the baffle assembly 424 correspond to one another, i.e., the position-limiting baffle 414 moves up and down between the supporting base 423 and the baffle assembly 424. Furthermore, the filter main plate 411, the filter side plates 412, and the upwardly flipped filter body 331 are combined to form a filtering area. The position-limiting baffle 414 moves between the supporting base 423 and the baffle assembly 424, thereby limiting the range of movement of the filter main plate 411. In addition to preventing the filter main board 411 from interfering with the staff in replacing the sample on the loading platform 22, it also prevents the filter main board 411 and the top of the filter side plate 412 from colliding with the top connector of the mirror arm 14, thereby damaging the filter main board 411 and the filter side plate 412.

[0062] Specifically, the fixing plate 421 and the side positioning plate 422 are fixedly mounted on one side of the mirror arm 14, and a movable groove 413 is provided on the back side of the filter main board 411, so that the filter main board 411 can be movably mounted on the side positioning plate 422. The distance between the two sets of filter side plates 412 on the surface of the filter main board 411 is adapted to the distance between the filter main bodies 331 on both sides of the loading platform 22. Therefore, when the loading platform 22 moves upward and the filter main body 331 flips upward around the fixed axis 31, the inner side of the filter main body 331 will be attached to the outer side of the filter side plate 412, and then the combination will block and filter harmful light such as ultraviolet rays, reduce stray light and optimize imaging conditions, so that the observer can obtain a clearer and more comfortable visual experience.

[0063] At the beginning, the limit baffle 414 is on the supporting bottom column 423 to prevent the distance between the filter main board 411 and the top surface of the sample loading platform 22 from being too close, which makes it inconvenient for the staff to place the sample slide on the sample loading platform 22. When the sample loading platform 22 moves up and down, the two sides of the sample loading platform 22 will press against the bottom of the filter side plate 412, thereby driving the filter main board 411 to move upward along the side positioning plate 422 until the limit baffle 414 is in contact with the baffle assembly 424. This is the maximum height of the rising of the sample loading platform 22. At the same time, the baffle assembly 424 limits the rising height of the filter main board 411 to prevent the top of the filter main board 411 from damaging other components on the microscope, thereby increasing the safety of the device.

[0064] Example 4

[0065] Reference Figure 5, which is the fourth embodiment of the present invention, provides an adjustable fluorescence microscope, the lifting device 25 includes a servo motor 251 arranged on the base 11, and a steering gear group 252 is provided on one side of the servo motor 251, and a control button 255 is provided on the other side of the servo motor 251, and a transmission screw 253 is meshed at one end of the steering gear group 252, and the transmission screw 253 is movably connected to the object loading platform 22. Specifically, the object loading platform 22 is rotatably connected to the transmission screw 253, for example, it can be achieved by a ball bearing, which is the prior art. A screw top plate 254 is provided at the top of the transmission screw 253, and the screw top plate 254 is fixedly connected to the mirror arm 14. The control button 255 is used to control the switch of the servo motor 251, and then the servo motor 251 drives the steering gear group 252 to drive the transmission screw 253 meshed with it to rotate, thereby controlling the up and down movement of the object loading platform 22 connected to the transmission screw 253, wherein the control button 255 and the servo motor 251 are both the prior art.

[0066] Reference Figure 1 、 Figure 6 A light source device 12 is provided on the top of the base 11, and the light source device 12 is located directly below the loading platform 22. A wide-angle eyepiece 15 is fixedly provided on one side of the mirror arm 14, and a charge coupler 16 is fixedly provided on the top side of the wide-angle eyepiece 15. An achromatic objective lens 17 is movably provided at the bottom of the wide-angle eyepiece 15. Focusing knobs 13 are movably provided on both sides of the base 11 for quickly adjusting the distance between the achromatic objective lens 17 and the specimen on the loading platform 22 so that the object image appears in the field of view. A control knob 28 is provided on one side of the bottom of the loading platform 22. The control knob 28 is used to control the forward and backward movement of the loading platform 22 and the left and right movement of the specimen clamp 23 on the loading platform 22, thereby adjusting the position of the slide so that it is located directly below the achromatic objective lens 17, which is convenient for the staff to observe the sample. The focusing knob 13 and the control knob 28 are both existing technologies and are not described here.

[0067] The specimen holder 23 includes a fixed clamp 231 fixedly arranged on the top of the loading platform 22 , a rotating clamp 233 is movably provided on one side of the fixed clamp 231 , a rotating handle 234 is fixedly provided on the outer side of the rotating clamp 233 , and a specimen slot 232 is provided on the inner side of the fixed clamp 231 .

[0068] Specifically, before using the fluorescence microscope, the fluorescence microscope is as follows: Figure 2As shown, the staff first holds the rotating handle 234 to open the rotating clamp 233, and then clamps the two sides of the slide into the inner specimen slot 232 of the fixing clamp 231, and then slowly releases the rotating handle 234 to fix the slide with the rotating clamp 233. At the same time, the specimen slot 232 and the inner side of the rotating clamp 233 are inclined, and the distance between the specimen slot 232 and the inner side of the rotating clamp 233 gradually decreases from bottom to top, which can restrict the four corners of the slide to its inner side, thereby fixing the top of the slide at the same time, further increasing the fixing stability of the specimen clamp 23 to the slide.

[0069] After the slide is fixed, the slot 26 is just located on the fixed shaft 31, and the filter body 331 is on one side of the limit column 27. Then, the control button 255 is pressed to start the servo motor 251, and the servo motor 251 drives the steering gear set 252 to rotate, driving the transmission screw 253 to rotate in the screw top plate 254, so that the loading platform 22 movably connected to the outside of the transmission screw 253 moves upward. At this time, the limit column 27 will squeeze the outside of the filter body 331, so that the filter body 331 rotates upward around the fixed shaft 31, and the loading platform 22 continues to move upward. When the filter body 331 is completely upright between the loading platform 22 and the limit column 27, the top of the loading platform 22 just contacts the bottom of the filter side plate 412, thereby driving the filter main board 411 to move upward along with the loading platform 22.

[0070] Then continue to control the loading platform 22 to move to the set distance, stop the servo motor 251 at this time, and then control the loading platform 22 to move back and forth through the control knob 28, and at the same time adjust the specimen clamp 23 to move left and right on the loading platform 22 until the slide is directly under the achromatic objective lens 17. Then the staff observes the cells of the load through the wide-angle eyepiece 15 and takes a picture of the situation on the slide through the charge coupler 16.

[0071] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the various embodiments of the present invention.

Claims

1. An adjustable fluorescence microscope, comprising a base (11), a mirror arm (14) arranged on the top of the base (11), and a loading platform (22) arranged on one side of the mirror arm (14), characterized in that: A lifting device (25) is provided on the top surface of the base (11), and one end of the loading platform (22) is movably connected to the lifting device (25); A fixed shaft (31) is provided on one side of the mirror arm (14), and the fixed shaft (31) passes through both sides of the loading platform (22). A first filter (33) is rotatably provided on the fixed shaft (31), and both sides of the loading platform (22) are fixed with limiting columns (27) for limiting the first filter (33) on both sides of the loading platform (22); when the lifting device (25) controls the loading platform (22) to move upward, the first filter (33) is blocked by the limiting columns (27) so as to turn upward around the fixed shaft (31) to form a filtering area; when the loading platform (22) moves downward, the first filter (33) automatically turns downward around the fixed shaft (31) under the action of gravity.

2. The adjustable fluorescence microscope according to claim 1, characterized in that: A through slot is formed between the limiting column (27) and the object loading platform (22), and the first optical filter (33) is inserted into the through slot.

3. The adjustable fluorescence microscope according to claim 1, characterized in that: A fixing device (37) is fixedly provided at one end of the fixing shaft (31) away from the mirror arm (14), and the fixing device (37) is used to limit and fix the first filter (33).

4. The adjustable fluorescence microscope according to claim 1, wherein: Both sides of the bottom of the object loading platform (22) are provided with slots (26) corresponding to the fixed shaft (31).

5. The adjustable fluorescence microscope according to claim 1, characterized in that: A control knob (28) is provided on one side of the bottom of the loading platform (22), and the lifting device (25) includes a servo motor (251) arranged on the base (11). A steering gear set (252) is provided on one side of the servo motor (251), and a transmission screw (253) is meshed at one end of the steering gear set (252). The transmission screw (253) is rotatably connected to the loading platform (22), and a screw top plate (254) is provided at the top end of the transmission screw (253), and the screw top plate (254) is fixedly connected to the mirror arm (14).

6. The adjustable fluorescence microscope according to claim 3, characterized in that: The fixing device (37) comprises a positioning baffle (371) fixedly arranged at one end of the fixed shaft (31); a ratchet clamping column (372) is movably provided inside the positioning baffle (371); a pawl (374) is fixedly provided at one end of the ratchet clamping column (372); a torsion spring (373) is provided on the outside of the ratchet clamping column (372); a one-way ratchet (376) corresponding to the pawl (374) is rotatably sleeved on the end of the fixed shaft (31) away from the mirror arm (14); a limiting clamping column (377) is fixedly provided on one side of the one-way ratchet (376); the limiting clamping column (377) is used to abut against the outside of the first optical filter (33) to limit and fix the first optical filter (33); the limiting clamping column (377) and the limiting column (27) are staggered with each other.

7. The adjustable fluorescence microscope according to claim 6, characterized in that: The limiting clamping column (377) comprises a connecting block fixedly connected to the one-way ratchet (376); a limiting rod is fixed to the end of the connecting block away from the one-way ratchet (376); the limiting rod extends to the outside of the first filter (33).

8. The adjustable fluorescence microscope according to claim 1, characterized in that: A positioning device (42) is fixedly provided on one side of the mirror arm (14), and a second filter (41) is movably provided on one side of the positioning device (42). The bottom of the second filter (41) contacts the object-carrying platform (22). When the object-carrying platform (22) moves upward, the second filter (41) moves upward along with the object-carrying platform (22) and forms a filtering area together with the first filter (33).

9. The adjustable fluorescence microscope according to claim 8, characterized in that: The positioning device (42) comprises a fixing plate (421) fixedly arranged on one side of the mirror arm (14); a side positioning plate (422) is fixedly arranged on the top of the fixing plate (421); supporting base columns (423) are provided on the top of the fixing plate (421) and are located on both sides of the side positioning plate (422); and baffle assemblies (424) are fixedly arranged on both sides of the side positioning plate (422).

10. The adjustable fluorescence microscope according to claim 9, characterized in that: The second filter (41) comprises a filter main plate (411) movably arranged on one side of the side positioning plate (422); filter side plates (412) are fixedly provided on both sides of the filter main plate (411) away from the side positioning plate (422); a movable groove (413) is provided on the side of the filter main plate (411) close to the side positioning plate (422); the movable groove (413) is adapted to the side positioning plate (422); a limiting baffle (414) is fixedly provided on the side of the filter main plate (411) close to the side positioning plate (422); the limiting baffle (414), the supporting base column (423), and the baffle assembly (424) correspond to each other.

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