Production method of complete protein biological feed

Abstract

The invention discloses a full-value protein biological feed production method, and relates to the technical field of protein feed production, the production method comprises the following steps: double-strain co-fermentation: cooling fermentable syrup to 38-42 DEG C, adjusting the pH value to 6.5-7.0, inoculating a mixed strain of Thermoactinomyces vulgaris and Candida utilis, fermenting for 18-24 hours under the condition that the dissolved oxygen is maintained to be 30-60%, and carrying out double-strain co-fermentation; in the process, the pH, dissolved oxygen and temperature are monitored in real time by an on-line sensor controlled by a PLC (Programmable Logic Controller), and materials are automatically supplemented; and membrane separation and concentration: enabling the fermentation liquor to pass through a PVDF (Polyvinylidene Fluoride) ultrafiltration membrane with the molecular weight cutoff of 10kDa-30kDa, and intercepting thalli and protein aggregates by a membrane concentration multiple of 5-10 to obtain a membrane concentrated solution with the protein concentration of more than or equal to 50g / L. According to the method, agricultural waste is efficiently converted into complete protein through double-bacterium synergistic fermentation, the problems that traditional single-bacterium fermentation is low in yield and unbalanced in nutrition are solved, and the protein yield is increased.

Description

Technical Field

[0001] This invention relates to the field of protein feed production technology, specifically a method for producing complete protein biological feed. Background Technology

[0002] Currently, animal protein feed mainly relies on traditional raw materials such as fishmeal and soybean meal, but this method suffers from significant resource fluctuations, high costs, unbalanced amino acid composition, and feed safety risks. Single-cell protein (SCP), as an alternative protein source, offers high protein content and controllable composition, but traditional processes suffer from low yield, high energy consumption, difficulties in downstream separation, and poor nutrient utilization. Therefore, there is an urgent need for a novel method for producing complete protein bio-feed that integrates efficient fermentation, fine separation, and low-loss drying.

[0003] Patent CN104957373B discloses a method for preparing feed that reduces heavy metal emissions in livestock and poultry manure. The patent improves the utilization rate of copper, iron, zinc and manganese in feed ingredients, and reduces the occurrence of gastric ulcer disease and improves the palatability of feed without adding trace elements such as copper, manganese and iron to pig feed. It also improves animal production performance while reducing heavy metal emissions in manure.

[0004] The aforementioned patents can meet the nutritional needs of pigs and promote their growth, while also reducing the emission of heavy metals in feces, the occurrence of digestive tract ulcers, and heavy metal pollution in manure. However, they lack technological accumulation in improving the protein yield in feed.

[0005] Therefore, this application proposes a method for producing complete protein bio-feed that can efficiently convert agricultural waste into complete protein, solving the problems of low yield and unbalanced nutrition in traditional single-strain fermentation. Summary of the Invention

[0006] The purpose of this invention is to provide a method for producing complete protein biological feed, in order to solve the technical problems mentioned in the background art, such as low yield, high energy consumption, difficulty in downstream separation, and poor nutrient utilization of single-cell protein (SCP) as an alternative protein source, although the protein content is high and the composition is controllable.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a method for producing complete protein biological feed, the method comprising the following steps:

[0008] Raw material pretreatment: Mix corn bran and sugarcane bagasse at a weight ratio of 1:1, pretreat with hot water at 80-95℃ for 30-60 minutes, cool to 50℃-60℃, add 0.5%-1.0% (w / w) of cellulase, and enzymatically hydrolyze for 2-4 hours under pH 5.0-6.0 conditions to obtain fermentable syrup;

[0009] Co-fermentation with two strains: The fermentable syrup is cooled to 38℃-42℃ and the pH is adjusted to 6.5-7.0. A mixed strain of Thermoactinomyces vulgaris and Candida utilis is inoculated and fermented for 18h-24h under dissolved oxygen conditions of 30%-60%. During the process, the pH, dissolved oxygen and temperature are monitored in real time by an online sensor controlled by PLC, and the feed is automatically replenished.

[0010] Membrane separation and concentration: The fermentation broth is passed through a PVDF ultrafiltration membrane with a molecular weight cutoff of 10kDa-30kDa to retain bacterial cells and protein aggregates at a membrane concentration ratio of 5-10 times, resulting in a membrane concentrate with a protein concentration ≥50g / L.

[0011] Preferably, the production method further includes:

[0012] Deep enzymatic hydrolysis and fine-tuning: Add 0.5%-1.5% (w / w) of trypsin and 0.2%-0.8% (w / w) of papain to the membrane concentrate, and enzymatically hydrolyze for 1-2 hours at 50-55℃ and pH 7.5-8.5. After enzymatic hydrolysis, add lysine and methionine fortifiers at a ratio of 2%-5% (w / w) of total protein and homogenize.

[0013] Preferably, the production method further includes:

[0014] Forming and granulation: The prepared protein solution is fed into a screw extruder granulator with a speed of 200r / min-400r / min and a feed temperature of ≤50℃. Raw feed pellets with a diameter of 2mm-5mm are produced through an adjustable die.

[0015] Microwave-vacuum coupled drying: Under vacuum conditions of 0.08MPa-0.1MPa, the raw feed pellets are dried with microwave power of 2kW-5kW for 20min-40min, so that the moisture content of the pellets is ≤8% and the loss of protein bioactive components is ≤5%.

[0016] Preferably, the production method further includes:

[0017] Online intelligent packaging: Using a sensor with a weight detection accuracy of ±1g and a nitrogen filling device, the dry granules are automatically packaged in a moisture-resistant and antioxidant composite film bag and the batch QR code is automatically printed.

[0018] Before packaging, an online quality inspection is designed: near-infrared spectroscopy is used to quickly analyze protein content and moisture, and the product can only be released if the prediction error is ≤2%.

[0019] Preferably, the process units in each step of the production method are connected to the SCADA system via industrial Ethernet to achieve centralized monitoring, automatic alarm, and MES-level production data traceability;

[0020] The SCADA system has functions such as automatic alarm for equipment faults, energy efficiency statistics, and OEE analysis.

[0021] Preferably, the cellulase system is a concentrated product of Trichoderma reesei fermentation, and the cellulase activity added per liter of reaction solution during enzymatic hydrolysis is not less than 5000U.

[0022] The online sensors also include turbidity sensors and flow sensors. The PLC dynamically adjusts the stirring rate and nutrient supply rate based on the PID algorithm to optimize fermentation efficiency.

[0023] Preferably, the ultrafiltration membrane module has a multi-channel spiral structure and employs an alternating backwashing and online chemical cleaning mode to ensure a membrane flux ≥ 50 L / (m²). 2 ·h).

[0024] Preferably, the fortifiers added after the deep enzymatic hydrolysis also include trace element chelates and prebiotics to improve the intestinal health of animals.

[0025] Preferably, the screw extrusion granulator is a twin-screw design, and the screw speed and back pressure can be adjusted to prepare feed pellets of different densities and shapes;

[0026] In the microwave-vacuum coupled drying process, the recovered water vapor is condensed to achieve ≥50% pre-treated water reuse.

[0027] Preferably, the online near-infrared spectroscopy quality detection achieves a protein content prediction error of ≤2% by comparing and establishing a multiple regression model;

[0028] The batch QR code contains fermenter number, production date, process parameters, and test results information for full traceability.

[0029] Compared with the prior art, the beneficial effects of the present invention are:

[0030] 1. This invention achieves efficient conversion of agricultural waste into complete protein through dual-strain synergistic fermentation, solving the problems of low yield and nutritional imbalance in traditional single-strain fermentation, and increasing protein production;

[0031] 2. This invention achieves precise control of protein content and amino acid ratio through the integration of ultrafiltration and deep enzymatic hydrolysis, solving the problems of amino acid loss during high-temperature concentration and high separation costs, shortening the fermentation cycle, and reducing production costs;

[0032] 3. This invention uses microwave-vacuum rapid drying to maintain the activity and nutrients of microorganisms, solving the problems of long drying time and severe damage to active ingredients caused by hot air drying, thus shortening the drying time and improving the retention rate of biological activity;

[0033] 4. This invention achieves automated, energy-saving, and traceable production through the intelligent integration of SCADA and MES, solving the problem of traditional production methods lacking online monitoring and full traceability, which makes it difficult to ensure stable product quality. It achieves stable product quality, environmental friendliness, high degree of automation, significantly improves breeding performance, and reduces breeding risks. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the production process of complete protein biological feed according to the present invention. Detailed Implementation

[0035] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] Example 1

[0037] Please see Figure 1A method for producing complete protein biological feed involves weighing corn bran and sugarcane bagasse (mass ratio 1:1) and adding them to a reaction vessel. Distilled water is added to adjust the moisture content to 55%. The mixture is heated to 90℃ and treated in a water bath for 40 minutes. After cooling to 55℃, the pH is adjusted to 5.5 using a 10% (w / v) sodium hydroxide and hydrogen chloride solution. Cellulase from Trichoderma reesei (activity 6500 U / g) is added at a ratio of 0.8%, and enzymatic hydrolysis is performed for 3 hours. The hydrolysate is then adjusted to pH 6.8 and cooled to 40℃. A mixed bacterial culture of Thermoactinomyces vulgaris (seed concentration 10⁷ CFU / ml) and Candida utilis (10⁷ CFU / ml) is inoculated at 0.5% (v / v). Fermentation is carried out in a fermenter under dissolved oxygen conditions controlled at 40% for 22 hours. A PLC system controls the temperature (maintaining 40℃) and pH fluctuations (6.6-6.9) in real time. The fermentation broth is then subjected to a 20kDa... The protein concentration was increased 6-fold using a PVDF membrane to obtain a concentrate with a protein concentration of 55 g / L. Trypsin (1.2%) and papain (0.5%) were added, and the mixture was enzymatically hydrolyzed at 52℃ and pH 8.0 for 90 min. Then, lysine (3.5%), methionine (2%), a CaCl2, MgSO4, and ZnSO4 chelate (0.5%), and fructooligosaccharides (1%) were added, and the mixture was stirred and homogenized for 10 min for enhanced formulation. The mixture was then granulated using a twin-screw extruder (temperature controlled at 45℃) to produce 4 mm diameter particles. These particles were then treated with microwave power (4 kW) under a vacuum of 0.09 MPa for 30 min and dried to a moisture content of 6.5%. The formulated solution was fed into an extruder, with the screw speed set to 3... 00 rpm, back pressure 2 MPa, mold diameter 3 mm; collect raw granules and send them to the dryer; set vacuum 0.09 MPa, microwave power 4 kW, dry for 30 min; sample and measure moisture content (oven method), ensuring ≤8%, and measure enzyme activity retention rate ≥90%; after drying, scan and detect using a near-infrared spectrometer (band 400–1000 nm), model error ≤2%; granules are automatically conveyed to an online checkweighing system (accuracy ±1g), and after passing the checkweighing, they enter a nitrogen filling and packaging machine; packaging bag: aluminum foil composite moisture-proof bag, with desiccant on the inner wall; automatically inkjet print batch QR code, including: production date, fermentation tank number, and main process parameters.

[0038] The above product was named A1 and used as the experimental group; at the same time, a control group A0 was set up, which was fermented with only Candida utilis single strain, with the rest of the process unchanged.

[0039] Furthermore, the general experimental methods and analytical techniques are as follows:

[0040] 1. Sample preparation and sampling

[0041] Each experimental group and control group were set up in triplicate (n=3). The products obtained under all experimental conditions were uniformly mixed according to the standard method and then sampled. After the samples were dried to a stable weight, they were finely ground in a mortar and passed through an 80-mesh sieve for subsequent analysis.

[0042] 2. Determination of chemical composition

[0043] Crude protein: determined by the Kjeldahl nitrogen method (GB / T 6432-2018), calculated as total nitrogen × 6.25;

[0044] Moisture content: dried at 105℃ (GB / T 6435-2014);

[0045] Crude fat: Soxhlet extraction method (GB / T 5009.6-2016);

[0046] Water-soluble protein: The content of soluble protein was determined according to the Lowry method;

[0047] Amino acid profile: After acid hydrolysis, quantification was performed using HPLC (Agilent 1200 system, ZORBAX Eclipse AAA column).

[0048] 3. Online monitoring and automated control verification

[0049] During the fermentation process, pH, DO, and temperature were monitored online by a METTLER TOLEDO InPro 3250i electrode, and the data was collected and fed back in real time by a PLC (Siemens S7-1200).

[0050] The near-infrared online mass spectrometer (Bruker MATRIX-F) is used for rapid detection of protein content and moisture before packaging, and comparison with laboratory measurements.

[0051] 4. Statistical Analysis

[0052] Results are expressed as mean ± standard deviation; one-way ANOVA was performed using SPSS 26.0 at a significance level of α = 0.05; differences between groups were analyzed by Tukey post-hoc test.

[0053] The results and analysis are shown in Table 1:

[0054] Table 1 Nutritional Analysis Results

[0055] index A1 (Double-strain) A0 (single bacteria) p-value Crude protein (%) 42.7±0.6 35.2±0.8 ＜0.01 Water-soluble protein (%) 32.1±0.5 21.9±0.7 ＜0.01 Total amino acid 468±12mg / g 377±15mg / g ＜0.01

[0056] Analysis: Co-fermentation with two microorganisms significantly increased protein content and the proportion of soluble protein, with a 24% increase in total amino acids.

[0057] Example 2

[0058] Please see Figure 1 A method for producing complete protein biological feed, based on Example 1, involves taking the same batch of fermentation broth and concentrating it 6× using three PVDF membranes of 10kDa, 20kDa, and 30kDa under the same operating conditions (TMP 1.0 bar, flow rate 1.5m / s), respectively, and designating them as groups B10, B20, and B30, and testing their protein rejection rate and flux.

[0059] Example 2 aims to compare the effects of ultrafiltration membranes with different molecular weight cutoffs on protein concentration efficiency. Starting from the same batch of fermentation broth, equal volumes were dispensed into three parallel ultrafiltration systems, each equipped with 10 kDa, 20 kDa, and 30 kDa PVDF membrane modules. All operating parameters were uniformly set: transmembrane pressure difference 1.0 bar, membrane surface flow rate 1.5 m / s, and concentration ratio 6. Instantaneous flux and rejection rate of each membrane were recorded during operation until a 6-fold concentration was achieved. After the experiment, the protein concentration and flux data of the three membrane concentrates were measured, and the protein rejection rate was calculated, thus determining that the 20 kDa ultrafiltration membrane possesses both high flux and superior rejection performance.

[0060] The comparison results of membrane concentration parameters are shown in Table 2:

[0061] Table 2 Comparison of Membrane Concentration Parameters

[0062] Sample number Retention rate % <![CDATA[Membrane flux L / m 2 ·h]]> protein concentration g / L B10 92.5%±1.2 36.2±2.1 56.4±1.0 B20 88.7±1.0 52.8±2.4 54.7±0.8 B30 79.6±1.5 67.1±3.0 48.2±1.3

[0063] Analysis: The 20kDa membrane strikes a balance between rejection rate and flux, making it the optimal process choice.

[0064] Example 3

[0065] Please see Figure 1 This invention discloses a method for producing complete protein bio-feed. Based on Example 1, the influence of protease combination on the degree of protein hydrolysis and amino acid composition was investigated. Using the 20 kDa membrane concentrate obtained in Example 1 as a base, three groups of enzymatic hydrolysis samples were prepared: Group C1 with only 1.0% trypsin, Group C2 with only 0.5% papain, and Group C3 with both 1.0% trypsin and 0.5% papain. Each group was enzymatically hydrolyzed for 1.5 hours at 50°C, pH 8.0, and 200 rpm. Samples were taken every 30 minutes during the hydrolysis process, and the degree of hydrolysis was determined using the O-phthaldialdehyde method. After the hydrolysis was completed, the samples were analyzed by HPLC to quantitatively determine the content of lysine, methionine, and other key amino acids.

[0066] Furthermore, the same concentrated solution was divided into three portions, forming three groups:

[0067] Group C1: Trypsin 1.0%;

[0068] Group C2: Papain 0.5%;

[0069] Group C3: Trypsin 1.0% + Papain 0.5%;

[0070] Under the same enzymatic hydrolysis conditions of 50℃, pH 8.0, 200 rpm, and 1.5 h, samples were taken for analysis (O-phehaldialdehyde method) to determine the degree of hydrolysis and amino acid composition.

[0071] The hydrolysis results are shown in Table 3:

[0072] Table 3 Hydrolysis Results

[0073] Group Degree of hydrolysis % Lysine content (mg / g) Methionine mg / g Phenylalanine mg / g C1 41.2±0.9 15.4±0.6 6.8±0.3 8.2 C2 36.3±1.1 13.6±0.5 6.1±0.4 7.4 C3 52.6±1.3 19.1±0.8 9.2±0.5 9.8

[0074] Analysis: The compound protease significantly improved the degree of protein hydrolysis and the content of target amino acids, indicating a significant synergistic effect.

[0075] Example 4

[0076] Please see Figure 1 A method for producing complete protein bio-feed, based on Example 1, involves taking wet granulated samples of the same batch and moisture content, placing one portion in a microwave-vacuum drying oven (0.09 MPa, 4 kW) and the other portion in a 60°C hot air drying oven. Microwave-vacuum drying is performed, and the time required for the sample moisture content to drop to 8% is recorded; simultaneously, hot air drying is performed for 8 hours until the sample moisture content stabilizes. After drying, the final moisture content, crude protein content, and retention rate of key enzyme activities of both groups of samples are measured to evaluate the efficiency of the two drying methods and their protective effect on active ingredients.

[0077] Furthermore, experimental group D1 was set up using the microwave-vacuum drying process of this application, while control group D0 was dried with hot air at 60°C for 8 hours;

[0078] The drying results are shown in Table 4:

[0079] Table 4 Drying Results

[0080] index D1 D0 Drying time (min) 35±2 480±10 Final moisture % 6.7±0.2 7.2±0.3 Enzyme activity retention rate % 94±1.5 62±2.0

[0081] Analysis: Microwave-vacuum drying significantly shortens drying time and improves enzyme activity retention, verifying the advantages of low-temperature rapid drying.

[0082] Example 5

[0083] Please see Figure 1This invention discloses a method for producing a complete protein biological feed. An animal feeding trial (weaned piglets) was conducted based on Example 1. Forty healthy 21-day-old weaned piglets were randomly divided into two groups: a control group (E0) fed a commercial complete feed, and an experimental group (E1) fed the complete protein biological feed prepared according to this invention, replacing 25% of the commercial feed. Both groups were fed continuously for 30 days. Feeding and water intake were uniformly managed during the experiment. Individual weights were weighed and recorded regularly, and the average daily weight gain and feed conversion ratio were calculated. The occurrence of diarrhea was observed and recorded daily, and the diarrhea rate was calculated. After the experiment, growth performance and health indicators were statistically analyzed to evaluate the practical application effect of the feed of this invention.

[0084] Further, experimental design:

[0085] Group Feed type Sample size Feeding cycle Key Indicators E0 Commercial complete feed 20 30 days Daily weight gain, feed conversion ratio, diarrhea rate E1 This application pertains to feed (A1). 20 30 days Same as above

[0086] The results of the feeding trial are shown in Table 5:

[0087] Table 5 Feeding Trial Results

[0088] index E1 E0 Average daily weight gain (g) 426±15 384±12 Meat-to-fat ratio 1.61±0.03 1.78±0.04 diarrhea rate 5.3%±1.1% 11.7%±1.5%

[0089] Analysis: The complete protein feed proposed in this application can effectively increase daily weight gain, improve feed conversion ratio, and reduce the risk of diarrhea.

[0090] Working principle: Agricultural wastes rich in cellulose, such as corn husks and sugarcane bagasse, are pretreated with hot water and hydrolyzed by cellulase to break down into fermentable monosaccharides and oligosaccharides, providing a sufficient carbon source for subsequent microbial growth.

[0091] By utilizing the synergistic fermentation of Thermoactinomyces vulgaris and Candida utilis, the thermophilic bacteria secrete cellulolytic enzymes and maintain yeast activity at high temperatures, while the yeast efficiently synthesizes proteins. The two complement each other to achieve high-concentration protein production.

[0092] The fermentation broth is first enriched with bacterial cells and protein aggregates through a PVDF ultrafiltration membrane, then the amino acid profile is optimized by deep enzymatic hydrolysis with trypsin and papain, and finally dehydrated and dried by microwave-vacuum coupling, maintaining the protein structure and biological activity in a low-temperature vacuum environment.

[0093] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A method for producing complete protein biological feed, characterized in that: The production method includes the following steps: Raw material pretreatment: Mix corn bran and sugarcane bagasse at a weight ratio of 1:1, pretreat with hot water at 80-95℃ for 30-60 minutes, cool to 50℃-60℃, add 0.5%-1.0% (w / w) of cellulase, and enzymatically hydrolyze for 2-4 hours under pH 5.0-6.0 conditions to obtain fermentable syrup; Co-fermentation with two strains: The fermentable syrup is cooled to 38℃-42℃ and the pH is adjusted to 6.5-7.

0. A mixed strain of Thermoactinomyces vulgaris and Candida utilis is inoculated and fermented for 18h-24h under dissolved oxygen conditions of 30%-60%. During the process, the pH, dissolved oxygen and temperature are monitored in real time by an online sensor controlled by PLC, and the feed is automatically replenished. Membrane separation and concentration: The fermentation broth is passed through a PVDF ultrafiltration membrane with a molecular weight cutoff of 10kDa-30kDa to retain bacterial cells and protein aggregates at a membrane concentration ratio of 5-10 times, resulting in a membrane concentrate with a protein concentration ≥50g / L.

2. The method for producing complete protein biological feed according to claim 1, characterized in that: The production method further includes: Deep enzymatic hydrolysis and fine-tuning: Add 0.5%-1.5% (w / w) of trypsin and 0.2%-0.8% (w / w) of papain to the membrane concentrate, and enzymatically hydrolyze for 1-2 hours at 50-55℃ and pH 7.5-8.

5. After enzymatic hydrolysis, add lysine and methionine fortifiers at a ratio of 2%-5% (w / w) of total protein and homogenize.

3. The method for producing complete protein biological feed according to claim 1, characterized in that: The production method further includes: Forming and granulation: The prepared protein solution is fed into a screw extruder granulator with a speed of 200r / min-400r / min and a feed temperature of ≤50℃. Raw feed pellets with a diameter of 2mm-5mm are produced through an adjustable die. Microwave-vacuum coupled drying: Under vacuum conditions of 0.08MPa-0.1MPa, the raw feed pellets are dried with microwave power of 2kW-5kW for 20min-40min, so that the moisture content of the pellets is ≤8% and the loss of protein bioactive components is ≤5%.

4. The method for producing complete protein biological feed according to claim 1, characterized in that: The production method further includes: Online intelligent packaging: Using a sensor with a weight detection accuracy of ±1g and a nitrogen filling device, the dry granules are automatically packaged in a moisture-resistant and antioxidant composite film bag and a batch QR code is automatically printed. Before packaging, an online quality inspection is designed: near-infrared spectroscopy is used to quickly analyze protein content and moisture, and the product can only be released if the prediction error is ≤2%.

5. The method for producing complete protein biological feed according to claim 1, characterized in that: The process units in each step of the production method are connected to the SCADA system via industrial Ethernet to achieve centralized monitoring, automatic alarm and MES-level production data traceability. The SCADA system has functions such as automatic alarm for equipment faults, energy efficiency statistics, and OEE analysis.

6. The method for producing complete protein biological feed according to claim 1, characterized in that: The cellulase system is a concentrated product of Trichoderma reesei fermentation, and the cellulase activity is not less than 5000U per liter of reaction solution during the enzymatic hydrolysis process. The online sensors also include turbidity sensors and flow sensors. The PLC dynamically adjusts the stirring rate and nutrient supply rate based on the PID algorithm to optimize fermentation efficiency.

7. The method for producing complete protein biological feed according to claim 1, characterized in that: The ultrafiltration membrane module has a multi-channel spiral structure and employs an alternating backwashing and online chemical cleaning mode to ensure a membrane flux ≥ 50 L / (m²). 2 ·h).

8. The method for producing complete protein biological feed according to claim 2, characterized in that: The fortifiers added after the deep enzymatic hydrolysis also include trace element chelates and prebiotics to improve the gut health of animals.

9. A method for producing complete protein biological feed according to claim 3, characterized in that: The screw extrusion granulator is a twin-screw design, and the screw speed and back pressure can be adjusted to produce feed pellets of different densities and shapes. In the microwave-vacuum coupled drying process, the recovered water vapor is condensed to achieve ≥50% pre-treated water reuse.

10. A method for producing complete protein biological feed according to claim 4, characterized in that: The online near-infrared spectroscopy quality detection achieves a protein content prediction error of ≤2% through comparison with a multivariate regression model. The batch QR code contains fermenter number, production date, process parameters, and test results information for full traceability.

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