Prebiotic liquid for livestock and poultry as well as preparation method and application of prebiotic liquid

Abstract

The invention relates to the technical field of prebiotic liquid for livestock and poultry, in particular to prebiotic liquid for livestock and poultry and a preparation method and application of the prebiotic liquid for livestock and poultry. The prebiotic liquid is prepared from radix astragali, herba leonuri, radix acanthopanacis senticosi, vitamin E, vitamin A, vitamin D, vitamin C and water according to a specific proportion. Wherein 1500g of the prebiotic liquid contains 350g-550g of astragalus membranaceus, 250g-450g of leonurus, 250g-450g of acanthopanax, 0.15 g-0. 3g of vitamin E, 2500-4500IU of vitamin A, 250-450IU of vitamin D, 50g-100g of vitamin C and the balance of water. The preparation method comprises the following steps: pretreating raw materials, extracting effective components of traditional Chinese medicinal materials, dissolving and adding vitamins, fixing the volume, uniformly mixing, sterilizing, sub-packaging and storing. Through scientific proportioning of the traditional Chinese medicinal materials and the vitamins, the intestinal health and organism immunity of livestock and poultry are improved, and meanwhile, the extraction rate of effective components of the traditional Chinese medicinal materials is increased, the stability of the vitamins is ensured, and the shelf life of the product is prolonged by optimizing an extraction process, controlling vitamin adding conditions, adopting pasteurization, sealing storage in a dark place and the like.

Description

Technical Field

[0001] This invention relates to the field of prebiotic liquid for livestock and poultry, and particularly to a prebiotic liquid for livestock and poultry, its preparation method, and its application. Background Technology

[0002] With the rapid development of animal husbandry, people have increasingly higher requirements for the quality and safety of livestock and poultry products. As a substance that can promote the growth and reproduction of beneficial intestinal flora, prebiotics have been widely used in livestock and poultry farming. At present, there are many types of prebiotic products on the market, but most of them have single functions and lack effective regulation of livestock and poultry immunity.

[0003] Traditional Chinese medicinal herbs have advantages such as being natural, safe, and having few side effects, giving them unique advantages in livestock and poultry farming. Astragalus membranaceus has effects such as enhancing immunity, anti-oxidation, and antiviral activity; Leonurus japonicus has effects such as promoting blood circulation, regulating menstruation, and promoting diuresis and reducing swelling; Acanthopanax senticosus has effects such as anti-fatigue, enhancing immunity, and regulating endocrine function. Combining these medicinal herbs with vitamins to prepare prebiotic liquids can fully leverage the synergistic effects of medicinal herbs and vitamins, improving the immunity and production performance of livestock and poultry. However, existing methods for preparing prebiotic liquids for livestock and poultry suffer from low extraction rates of effective components from medicinal herbs, poor vitamin stability, and short product shelf life.

[0004] Therefore, developing a prebiotic liquid for livestock and poultry with high extraction rate of effective components from traditional Chinese medicine, good vitamin stability, and long shelf life, as well as its preparation method, is of great practical significance. Summary of the Invention

[0005] To achieve the above objectives, the present invention adopts the following technical solution: The 1500g of the prebiotic liquid contains the following substances: Astragalus membranaceus 350g~550g, Leonurus japonicus 250g~450g, Acanthopanax senticosus 250g~450g, Vitamin E - 0.15g~0.3g, Vitamin A - 2500~4500IU, Vitamin D3 - 250~450IU, Vitamin C - 50g~100g, the remainder is water.

[0006] Furthermore, the 1500g of the prebiotic liquid contains the following substances: Astragalus membranaceus 350g~450g, Leonurus japonicus 250g~350g, Acanthopanax senticosus 250g~350g, Vitamin E - 0.15g~0.25g, Vitamin A - 2500~3500IU, Vitamin D3 - 250~350IU, Vitamin C - 50g~80g, the remainder is water.

[0007] Furthermore, the 1500g of the prebiotic liquid contains the following substances: Astragalus membranaceus 380g~400g, Leonurus japonicus 280g~320g, Acanthopanax senticosus 280g~320g, Vitamin E - 0.22g~0.23g, Vitamin A - 2800~3200IU, Vitamin D3 - 280~320IU, Vitamin C - 60~72g, the remainder is water.

[0008] Furthermore, the vitamin A is a vitamin A palmitate preparation, and the vitamin D3 is a water-soluble vitamin D3 microcapsule preparation to improve its stability.

[0009] This invention also provides a method for preparing prebiotic liquid for livestock and poultry; The preparation method of the prebiotic liquid for livestock and poultry includes the following steps: Raw material pretreatment: Take Astragalus membranaceus, Leonurus japonicus and Acanthopanax senticosus, rinse with purified water to remove impurities, drain and cut into 2-3 mm thin slices or crush into coarse powder that passes through a 10-mesh sieve to facilitate subsequent extraction.

[0010] Extraction of active ingredients from Chinese medicinal herbs: Pre-treated Chinese medicinal herbs are placed in an extraction tank, and 8-10 times the volume of purified water is added. The herbs are soaked at room temperature for 30-40 minutes to allow them to fully absorb water and swell. After boiling, the mixture is simmered for 40-50 minutes, stirring every 10 minutes to ensure even heating. The first filtrate is collected by filtering through a 300-mesh filter. 5-6 times the volume of purified water is added to the residue, and the mixture is heated again to a boil and simmered for 25-30 minutes. The second filtrate is collected by filtering. The two filtrates are combined and centrifuged at 3000 rpm for 10 minutes or filtered through a 300-mesh precision filter for clarification to obtain the Chinese medicinal herb extract. This two-stage decoction method can improve the dissolution rate of active ingredients such as Astragalus polysaccharides, Leonurus flavonoids, and Acanthopanax senticosus glycosides.

[0011] Vitamin dissolution and addition: Cool the herbal extract to below 40℃ to avoid damaging the vitamin activity due to high temperature; mix vitamin E, vitamin A preparation, and vitamin D3 microcapsule preparation. If vitamin E is an oil, add 5-10mL of purified water, stir to emulsify, and then mix. Then add a small amount of extract and stir until dispersed or dissolved to obtain a vitamin premix; add vitamin C to the extract and stir until dissolved; then pour the vitamin premix into the extract and stir at 300r / min for 5-10 minutes until homogeneous.

[0012] Adjust volume and mix: Add purified water to the above solution until the total mass reaches 1500g, and continue stirring for 10-15 minutes to ensure that all components are evenly dispersed.

[0013] Sterilization process: The prebiotic liquid after being brought to a fixed volume is kept at 65~70℃ for 30 minutes to kill microorganisms in the solution, while avoiding the degradation of vitamins and active ingredients of traditional Chinese medicine caused by high temperature.

[0014] Dispensing and storage: After sterilization, quickly cool the solution to room temperature and dispense it into light-proof sealed bottles using a quantitative filling machine under aseptic conditions. After sealing, label the bottles. The finished product should be stored in a cool, dry place away from light and sealed. The storage temperature should be ≤25℃. The shelf life is 6 months. Light-proof storage can reduce the risk of vitamin photo-oxidation and extend the shelf life.

[0015] The present invention has the following beneficial effects: This invention provides a prebiotic liquid for livestock and poultry, which scientifically combines traditional Chinese medicinal herbs such as Astragalus membranaceus, Leonurus japonicus, and Acanthopanax senticosus with various vitamins. It combines the immunomodulatory effects of traditional Chinese medicine with the nutritional supplementation function of vitamins, improving intestinal health and immunity in livestock and poultry, and enhancing their production performance. The extraction of the medicinal herbs employs a two-stage decoction method, with optimized parameters such as soaking time, decoction time, and water addition, which increases the dissolution rate of active ingredients such as Astragalus polysaccharides, Leonurus japonicus flavonoids, and Acanthopanax senticosus glycosides. Before adding vitamins, the extract is cooled to below 40°C to prevent the oxidation and inactivation of vitamins A, E, and C due to high temperatures, especially reducing the decomposition of vitamin C. Fat-soluble vitamins are pretreated to improve their dispersibility and stability in solution. Pasteurization, rather than high-temperature sterilization, is used to reduce the destruction of active ingredients in the medicinal herbs and vitamins, while ensuring the product's microbial safety. The finished product is stored in a light-proof, sealed container to reduce the risk of vitamin photo-oxidation and extend the shelf life to 6 months. Detailed Implementation

[0016] The embodiments of the present invention are described in detail below, and are intended to explain the present invention, but should not be construed as limiting the present invention.

[0017] This invention provides a prebiotic liquid for livestock and poultry, wherein 1500g of the prebiotic liquid contains the following substances: Astragalus membranaceus 350g~550g, Leonurus japonicus 250g~450g, Acanthopanax senticosus 250g~450g, Vitamin E - 0.15g~0.3g, Vitamin A - 2500~4500IU, Vitamin D3 - 250~450IU, Vitamin C - 50g~100g.

[0018] This invention also provides a method for preparing prebiotic liquid for livestock and poultry: Take Astragalus membranaceus, Leonurus japonicus, and Acanthopanax senticosus, rinse with purified water to remove impurities, drain, and then cut into 2-3 mm thin slices or crush into coarse powder that passes through a 10-mesh sieve for easier subsequent extraction. The pretreated Chinese medicinal materials are placed into an extraction tank, and 8-10 times the amount of purified water is added. The mixture is soaked at room temperature for 30-40 minutes to allow the materials to fully absorb water and swell. After boiling, the mixture is simmered for 40-50 minutes, stirring every 10 minutes to ensure even heating. The first filtrate is collected by filtering through a 300-mesh filter. 5-6 times the amount of purified water is added to the residue, and the mixture is heated again to a boil and simmered for 25-30 minutes. The second filtrate is collected by filtering. The two filtrates are combined and centrifuged at 3000 rpm for 10 minutes or filtered through a 300-mesh precision filter for clarification to obtain the Chinese medicinal material extract. The two-stage decoction method can improve the dissolution rate of active ingredients such as Astragalus polysaccharides, Leonurus flavonoids, and Acanthopanax senticosus glycosides. Cool the herbal extract to below 40℃ to avoid damaging vitamin activity due to high temperature; mix vitamin E, vitamin A preparation, and vitamin D3 microcapsule preparation. If vitamin E is an oil, add 5-10 mL of purified water, stir to emulsify, and then mix. Add a small amount of extract and stir until dispersed or dissolved to obtain a vitamin premix; add vitamin C to the extract and stir until dissolved; then pour the vitamin premix into the extract and stir at 300 r / min for 5-10 minutes until homogeneous. Add purified water to the above solution until the total mass reaches 1500g, and continue stirring for 10-15 minutes to ensure that all components are evenly dispersed; The prebiotic solution, after being brought to a fixed volume, is kept at 65-70°C for 30 minutes to kill microorganisms in the solution, while avoiding the degradation of vitamins and active ingredients of traditional Chinese medicine caused by high temperature. After sterilization, the solution is quickly cooled to room temperature and dispensed into light-proof sealed bottles using a quantitative filling machine under aseptic conditions. After sealing, the bottles are labeled. The finished product should be stored in a cool, dry place away from light and sealed, with a storage temperature of ≤25℃. The shelf life is 6 months. Storage away from light can reduce the risk of vitamin photo-oxidation and extend the shelf life.

[0019] The first embodiment of this application is as follows: Take 550g of Astragalus membranaceus, 450g of Leonurus japonicus, 450g of Acanthopanax senticosus, 0.3g of Vitamin E, 4500IU of Vitamin A, 450IU of Vitamin D3, and 100g of Vitamin C; Follow steps S1-S6 to cool the Chinese herbal extract to 40°C, stirring for 15 minutes until homogeneous, and pasteurizing at 70°C. In a stress model involving 100 laying hens, after 7 days of feeding, the egg production rate increased from 70% to 80%, the proportion of soft-shelled eggs decreased from 15% to 8%, feed intake increased by 12%, and the diarrhea rate decreased from 25% to 12%. The second embodiment of this application is as follows: Take 450g of Astragalus membranaceus, 350g of Leonurus japonicus, 350g of Acanthopanax senticosus, 0.25g of Vitamin E, 3500IU of Vitamin A, 350IU of Vitamin D3, and 80g of Vitamin C; Follow steps S1-S6 to cool the Chinese herbal extract to 38°C, stirring for 12 minutes until homogeneous, and pasteurizing at 68°C. In a stress model involving 100 laying hens, feeding for 7 days increased the egg production rate from 70% to 82%, reduced the proportion of soft-shelled eggs from 15% to 6%, increased feed intake by 16%, and reduced the diarrhea rate from 25% to 10%.

[0020] The third embodiment of this application is as follows: Take 400g of Astragalus membranaceus, 320g of Leonurus japonicus, 320g of Acanthopanax senticosus, 0.23g of Vitamin E, 3200IU of Vitamin A, 3320IU of Vitamin D, and 72g of Vitamin C; Follow steps S1-S6 to cool the Chinese herbal extract to 35°C, stirring for 10 minutes until homogeneous, and pasteurizing at 65°C. In a stress model involving 100 laying hens, feeding for 7 days increased the egg production rate from 70% to 90%, reduced the proportion of soft-shelled eggs from 15% to 4%, increased feed intake by 20%, and reduced the diarrhea rate from 25% to 7%.

[0021] The present invention also provides an application of prebiotic liquid for livestock and poultry, used to prepare livestock and poultry feed additives, with an addition amount of 0.5% to 2% of the feed mass, suitable for chickens and poultry.

[0022] To verify the efficacy of the drug, the following experiment was conducted, and the results are as follows: The experimental animals were 20-day-old healthy commercial Lohmann chicks, purchased from Luoyang Chundu Modern Chicken Farm at 1 day old. They were fed a complete chick feed provided by the farm without antibiotics, with free access to water. After 19 days of pre-feeding, maternal antibodies were negative by agar diffusion precipitation test (AGP), and they were then introduced into the experiment. No vaccines, bacteria, or drugs were administered during the pre-feeding period. 150 chicks were randomly divided into a control group, a positive control group, and a drug control group; 30 chicks in each group were housed in isolated groups. Infectious bursal disease (IBD) standard toxic strain IBDB87 (derived from the China Institute of Veterinary Drug Control) was inoculated at 0.5g / chick (except for the blank control group), simultaneously via eye drops and nasal drops. The chicks were weighed before challenge. The blank control group was housed under the same conditions as the other two groups. 24 hours after inoculation with IBDB87, the chicks began to exhibit typical clinical symptoms such as lethargy, decreased feed intake, and diarrhea. At this point, medication was administered according to the dosage in Table 1 for 5 consecutive days. The effectiveness and relative weight gain of each group are shown in Table 1: Table 1. Efficacy of this drug in artificially infected chicks with IBD: The efficacy rates of this drug in all treatment groups and other drug control groups were significantly higher than those in the positive control group. The weight gain was also significantly higher than that of the positive control group, indicating that the drug had different degrees of therapeutic effect on infectious bursal disease in chickens in different treatment groups. The efficacy rate of the high and medium dose groups was significantly higher than that of the low dose group (0.01 < P < 0.05), while there was no significant difference in efficacy rate and weight gain between the high and medium dose groups. The recommended dosage of this drug for chickens is: mixed with drinking water, 0.3g per 1L of water, for 5 consecutive days.

[0023] Although the disclosed prebiotic liquid for livestock and poultry, its preparation method and application have been specifically described with reference to the embodiments, the embodiments described above are illustrative and not restrictive. All variations and modifications are within the scope of the invention without departing from its spirit and scope.

Claims

1. A prebiotic liquid for livestock and poultry, characterized in that, The 1500g of the prebiotic liquid contains the following substances: Astragalus membranaceus 350g~550g, Leonurus japonicus 250g~450g, Acanthopanax senticosus 250g~450g, Vitamin E - 0.15g~0.3g, Vitamin A - 2500~4500IU, Vitamin D3 - 250~450IU, Vitamin C - 50g~100g, the remainder is water.

2. The prebiotic liquid for livestock and poultry as described in claim 1, characterized in that, The 1500g of the prebiotic liquid contains the following substances: Astragalus membranaceus 350g~450g, Leonurus japonicus 250g~350g, Acanthopanax senticosus 250g~350g, Vitamin E - 0.15g~0.25g, Vitamin A - 2500~3500IU, Vitamin D3 - 250~350IU, Vitamin C - 50g~80g, the remainder is water.

3. The prebiotic liquid for livestock and poultry as described in claim 2, characterized in that, The 1500g of the prebiotic liquid contains the following substances: Astragalus membranaceus 380g~400g, Leonurus japonicus 280g~320g, Acanthopanax senticosus 280g~320g, Vitamin E - 0.22g~0.23g, Vitamin A - 2800~3200IU, Vitamin D3 - 280~320IU, Vitamin C - 60~72g, the remainder is water.

4. The prebiotic liquid for livestock and poultry as described in claim 3, characterized in that, The vitamin A is a vitamin A palmitate preparation, and the vitamin D3 is a water-soluble vitamin D3 microcapsule preparation.

5. A method for preparing a prebiotic liquid for livestock and poultry, characterized in that, Includes the following steps: S1: Raw material pretreatment: Take Astragalus membranaceus, Leonurus japonicus and Acanthopanax senticosus, rinse with purified water to remove impurities, drain and cut into 2-3 mm thin slices or crush into coarse powder that passes through a 10-mesh sieve to facilitate subsequent extraction; S2: Extraction of effective components from Chinese medicinal materials: Put the pretreated Chinese medicinal materials into the extraction tank, add 8-10 times the amount of purified water, soak at room temperature for 30-40 minutes, heat to boiling and then simmer for 40-50 minutes, filter through a 300-mesh filter to collect the first filtrate, add 5-6 times the amount of purified water to the residue, heat to boiling again and then simmer for 25-30 minutes, filter to collect the second filtrate, combine the two filtrates and clarify to obtain the Chinese medicinal material extract; S3: Vitamin dissolution and addition: Cool the Chinese herbal extract to below 40°C, mix vitamin E, vitamin A preparation and vitamin D3 microcapsule preparation, add a small amount of extract and stir until dispersed or dissolved to obtain vitamin premix. Add vitamin C to the extract and stir until dissolved. Then pour the vitamin premix into the extract and stir for 5-10 minutes until uniform. S4: Adjust volume and mix: Add purified water to the above solution until the total mass reaches 1500g, and stir for 10-15 minutes until homogeneous; S5: Sterilization treatment: Pasteurization process, maintained at 65~70℃ for 30 minutes; S6: Dispensing and Storage: After cooling to room temperature, dispense into light-proof sealed bottles under aseptic conditions and store in a sealed container.

6. The method for preparing prebiotic liquid for livestock and poultry as described in claim 5, characterized in that, The clarification process in step S2 is to centrifuge at 3000 r / min for 10 minutes or to filter using a 300-mesh precision filter.

7. The method for preparing prebiotic liquid for livestock and poultry as described in claim 5, characterized in that, In step S3, vitamin E is an oil-based preparation. It is added to 5-10 mL of purified water, stirred and emulsified, and then mixed with vitamin A preparation and vitamin D3 microcapsule preparation.

8. The method for preparing prebiotic liquid for livestock and poultry as described in claim 5, characterized in that, The stirring speed in step S3 is 300 r / min.

9. The method for preparing prebiotic liquid for livestock and poultry as described in claim 5, characterized in that... In step S6, the finished product is stored at a temperature of ≤25℃ and has a shelf life of 6 months.

10. The use of the prebiotic liquid for livestock and poultry as described in any one of claims 1-3, characterized in that, Used to prepare livestock and poultry feed additives, the addition amount is 0.5% to 2% of the feed mass, and it is suitable for chickens and poultry.

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