A dandruff control and oil control composition, preparation method and application

Abstract

The present application relates to a kind of anti-dandruff oil-control composition, preparation method and application.The anti-dandruff oil-control composition includes by mass percentage: anti-dandruff maintenance factor 0.5-1.5%, oil-control factor 0.5-1.5%, guar gum 0.1-0.5%, emulsified silicone oil 1.5-4.5%, sodium chloride 0.5-1.5%, surfactant 15-31%, suspending agent 3-7%, preservative 0.5-1.5%, chelating agent 0.01-0.1%, the rest is solvent;Wherein, the oil-control factor includes 5% (w / v) 10-hydroxy decanoic acid;Anti-dandruff maintenance factor includes 99.5% bacillus fermentation product and 0.5% phenoxyethanol, and the bacillus fermentation product includes 1% (w / v) epidermal repair polysaccharide.The oil-control factor of the present application and the bacillus fermentation product containing epidermal repair polysaccharide are synergized from multiple angles, and the dandruff control ability of washing and protecting product is comprehensively improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of hair care products, in particular to a dandruff and oil control composition, a preparation method and application. BACKGROUND

[0002] In recent years, the concept of hair care has been continuously upgraded, and the concept of skin care type hair care has become increasingly popular. Under this background, consumers' awareness of scalp care has undergone a profound transformation, from simply focusing to actively taking action, and giving unprecedented attention to scalp health. This transformation has directly driven the development of the head hair care market, making it show a "total growth, subdivision upgrade" steady situation. Specifically, the efficacy of hair care products is advancing from basic deep cleaning and softness to dandruff control, oil control, hair loss prevention, and hair care. From the actual choices of consumers, the demand for dandruff control is particularly prominent.

[0003] Dandruff is a chronic, recurrent, and relatively common scalp problem. Compared with normal scalp, the appearance of thin flakes of dandruff on the scalp or in the hair is dandruff. Under normal circumstances, the shedding and regeneration of epidermal cells are in dynamic balance, and fully keratinized mature keratinocytes aggregate to form clusters with a diameter of less than 0.2 mm that are not visible to the naked eye. The visible keratinocyte clusters are considered dandruff, and the pathological examination shows that the keratinocytes are not fully keratinized, overgrown, and arranged irregularly. The scales can be observed under a microscope. In addition to flaky desquamation, the main clinical manifestations of dandruff are itching, strong dryness and tightness of the scalp, stinging, and subclinical inflammatory changes. At present, more than 50% of the world's population is affected by dandruff problems, with young people being the most common population and the disease being more severe. Generally, the incidence of men is higher than that of women, while the incidence of the elderly and children is lower.

[0004] Although the hair care products on the market are updated generation after generation, most of them have many deficiencies in dandruff control. The main reasons are as follows: most hair care products mainly rely on chemical dandruff agents such as zinc pyrithione, piroctone olamine, and sulfur. These chemical dandruff agents mainly rely on inhibiting the activity of Malassezia to achieve dandruff control. On the one hand, chemical dandruff agents can easily lead to drug resistance of Malassezia, resulting in poor long-term use effect. On the other hand, there are many factors affecting dandruff, and the pathogenesis is complex, involving scalp lipid, scalp microecology, scalp physiology, immune antioxidant, and many other factors. Relying solely on inhibiting Malassezia to control dandruff is only treating the symptoms and not the root cause. In addition, chemical dandruff agents have a large irritating effect on the scalp, which can easily cause scalp inflammation, thereby disrupting the ecological balance of the scalp and increasing dandruff. SUMMARY

[0005] Based on this, the purpose of the present application is to overcome the defects or deficiencies of the prior art, and to provide a dandruff control composition which has significant dandruff control and oil control effects and has low irritation.

[0006] The present application is implemented by the following technical solutions:

[0007] A dandruff control composition, the composition is composed of the following components in parts by weight:

[0008] 0.5-1.5 parts of dandruff maintenance factor, 0.5-1.5 parts of oil control factor, 0.1-0.5 parts of guar gum, 1.5-4.5 parts of emulsified silicone oil, 0.5-1.5 parts of sodium chloride, 15-31 parts of surfactant, 3-7 parts of suspending agent, 0.5-1.5 parts of preservative, 0.01-0.1 parts of chelating agent, 56-74 parts of water;

[0009] The oil control factor includes 10-hydroxy decanoic acid with a mass ratio of 5%.

[0010] The dandruff maintenance factor includes 99.5% Bacillus fermentation product and 0.5% phenoxyethanol, and the Bacillus fermentation product is prepared by fermentation of Bacillus subtilis and / or Bacillus subtilis with sucrose as the substrate. Paenibacillus edaphicus Bacillus subtilis The dandruff maintenance factor includes 99.5% Bacillus fermentation product and 0.5% phenoxyethanol, and the Bacillus fermentation product is prepared by fermentation of Bacillus subtilis and / or Bacillus subtilis with sucrose as the substrate.

[0011] Compared with the prior art, the oil control factor in the dandruff control composition of the present application and the Bacillus fermentation product containing epidermal repair polysaccharide synergistically act from multiple angles such as repairing skin barrier, inhibiting inflammation of the scalp, removing free radicals, inhibiting Malassezia, and inhibiting sebum secretion, forming a complete dandruff control system, and comprehensively improving the dandruff control ability of the washing and caring product, thereby providing a more efficient and comprehensive scalp care solution for consumers.

[0012] In an embodiment, the preparation method of the Bacillus fermentation product comprises the following steps:

[0013] (1) Activated culture of Bacillus subtilis and / or Bacillus subtilis to obtain activated strains;

[0014] (2) picking single colonies of the activated strains of step (1) into LGS medium, and culturing at 25-30°C for 36-60h to obtain a seed solution of the strain;

[0015] (3) preparing a fermentation medium with sucrose as the substrate, inoculating the seed solution of the strain obtained in step (2) into the fermentation medium, and culturing at 25-30°C for 36-60h to obtain the Bacillus fermentation product.

[0016] In an embodiment, the inoculation volume of the seed solution of the strain is 1-3% (v / v). ​

[0017] In an embodiment, the Bacillus fermentation product includes epidermis repair polysaccharide with a mass ratio of 0.5-1.5%, a molecular weight of 5.8-6.2 MDa, and a structural formula as shown below:

[0018] .

[0019] In an embodiment, the surfactant includes one or more of sodium fatty alcohol polyoxyethylene ether sulfate, coconut methyl monoethanolamide, laureth-6 carboxylic acid mixture, and cocamidopropyl betaine.

[0020] In an embodiment, the weight percentage of sodium fatty alcohol polyoxyethylene ether sulfate, coconut methyl monoethanolamide, laureth-6 carboxylic acid mixture, and cocamidopropyl betaine is 10-20%, 1-3%, 2-4%, and 2-4%, respectively.

[0021] In an embodiment, the preservative includes Mikrokill COS, and the suspending agent includes 3D cellulose gum.

[0022] The present application also provides a preparation method of the anti-dandruff and oil control composition, including the following steps:

[0023] S1, uniformly dispersed B phase is added to A phase, stirring and heating to 75-80℃, and holding stirring until each component is completely dissolved;

[0024] S2, the mixed solution prepared in step S1 is cooled to 68-72℃, and each raw material of C phase is sequentially added, and stirring and cooling are performed;

[0025] S3, the mixed solution prepared in step S2 is cooled to 44-46℃, and uniformly dispersed D phase is added, and stirring is performed for 5-15 min;

[0026] S4, E phase is added to the mixed solution prepared in step S3, and stirring and cooling are performed;

[0027] S5, the mixed solution prepared in step S4 is cooled to 41-43℃, F phase is added, and stirring is performed for 4-10 min;

[0028] S6, G phase is added to the mixed solution prepared in step S5, and the pH is adjusted to 5.5-6.5;

[0029] S7, H phase is added to the mixed solution prepared in step S6, and the anti-dandruff and oil control composition is prepared;

[0030] In the present application, the raw material of A phase includes 0.01-0.1 parts of a chelating agent, 10-20 parts of sodium fatty alcohol polyoxyethylene ether sulfate, 0.04 parts of citric acid monohydrate, and 50-55 parts of water.

[0031] The B-phase raw materials include: 0.1-0.5 parts of guar gum, 1-5 parts of water;

[0032] The C-phase raw materials include: 1-3 parts of cocamide MEA, 2-4 parts of laureth-6 carboxylic acid mixture, 2-4 parts of cocamidopropyl betaine;

[0033] The D-phase raw materials include: 3-7 parts of suspending agent, 6-14 parts of water;

[0034] The E-phase raw materials include: 1.5-4.5 parts of emulsified silicone oil;

[0035] The F-phase raw materials include: 0.5-1.5 parts of anti-dandruff care factor, 0.5-1.5 parts of oil control factor;

[0036] The G-phase raw materials include: 0.03-0.06 parts of citric acid monohydrate;

[0037] The H-phase raw materials include: 0.5-1.5 parts of preservative, 0.5-1.5 parts of sodium chloride.

[0038] The application also provides use of the anti-dandruff and oil control composition in the preparation of a hair care product.

[0039] In an embodiment, the hair care product includes shampoo, hair conditioner, hair mask, and scalp serum.

[0040] For better understanding and implementation, the application is described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF DRAWINGS

[0041] Figure 1 To test the inhibition rate of the anti-dandruff care factor in Example 1 on SLS-induced HaCaT cell death.

[0042] Figure 2 To test the binding effect of the anti-dandruff care factor in Example 1 with HaCaT cells.

[0043] Figure 3 To test the effect of the anti-dandruff care factor in Example 1 on the expression of MCP-1 in inflammatory cells.

[0044] Figure 4 To test the effect of the anti-dandruff care factor in Example 1 on the expression of IL-1β in inflammatory cells.

[0045] Figure 5 To test the effect of the anti-dandruff care factor in Example 1 on the expression of TNF-ɑ in inflammatory cells.

[0046] Figure 6 To test the effect of the anti-dandruff care factor in Example 1 on the expression of IL-6 in inflammatory cells.

[0047] Figure 7To test the effect of the anti-dandruff care factor in Example 1 on free radical scavenging; A is superoxide anion radical, B is hydroxyl radical, and C is DPPH radical.

[0048] Figure 8 To test the soothing effect of the anti-dandruff care factor in Example 1.

[0049] Figure 9 To test the short-term oil control effect of the oil control factor of Example 2.

[0050] Figure 10 To test the long-term oil control effect of the oil control factor of Example 2.

[0051] Figure 11 To test the efficacy of the oil control factor of Example 2 in reducing skin irritation.

[0052] Figure 12 To test the anti-dandruff effect of the anti-dandruff oil control composition in Example 3. DETAILED DESCRIPTION

[0053] In order to solve the problems of strong irritation and easy to cause Malassezia to develop drug resistance of chemical anti-dandruff agents, the existing scheme uses biological anti-dandruff polypeptide (produced by Hangzhou Maan New Material Co., Ltd., product model ADP-2000) to replace chemical anti-dandruff agents to prepare care products. The biological anti-dandruff polypeptide is mixed by Bacillus fermentation product, water and propylene glycol, and the anti-dandruff effect is realized by using the protein or polypeptide in the Bacillus fermentation product to inhibit Malassezia, compared with chemical anti-dandruff agents, the biological anti-dandruff polypeptide is more mild, does not irritate the scalp and has long antibacterial time.

[0054] However, there are many factors influencing dandruff and they are interrelated. First, there is a close mutual influence relationship between dandruff and the scalp sebum film. Most Malassezia are lipid-dependent, which lack fatty acid synthase gene and have a large number of lipase coding genes, so they can use host-derived lipids as their own nutrient source, decompose triglycerides and saturated fatty acids in sebum into pro-inflammatory unsaturated fatty acids, which not only cause itching of the scalp, but also bind to N-methyl-D-aspartate receptors to cause calcium ions to flow into, leading to hyperplasia and abnormal keratinization of keratinocytes and causing the production of scalp flaky scales, that is, the increase of oil helps to promote the proliferation of Malassezia, inhibit the growth of beneficial microorganisms, and further promote the growth of dandruff. In addition, the process of dandruff shedding will block the hair follicle orifice, affecting the normal excretion of the sebaceous gland. The blocked hair follicle orifice will stimulate the sebaceous gland to secrete more oil in compensation, trying to "flush out" the blockage, resulting in an oilier scalp.

[0055] However, the main function of the biological anti-dandruff polypeptide is to inhibit the activity of Malassezia, and it does not have the oil control effect, and needs to be combined with other oil control methods. The current oil control method mainly realizes oil control by reducing the addition amount of silicone oil, oil, conditioning agent, or by increasing the addition amount of surfactant to enhance the oil removal capacity, but both methods have obvious problems. Reducing the addition amount of silicone oil, oil, conditioning agent will cause the hair to not be well maintained during washing, and is easy to cause the hair to be frizzy and damaged, and such products are high in price and have limited antibacterial effect. Increasing the addition amount of surfactant can improve the oil removal effect, but it is easy to cause excessive cleaning and cause the stratum corneum to become thin and the water saturation to decrease, so that the scalp becomes sensitive, rough, and the resistance is reduced, and thus more dry hair, scalp itching, hair loss and other phenomena occur. And the existing surfactant has strong irritability, which can stimulate cells to produce inflammatory factors, cause cell apoptosis, and finally form scales, resulting in an increase in dandruff.

[0056] In order to avoid the addition amount of silicone oil and other conditioning agents from being reduced to cause the hair to be frizzy and damaged, and to avoid the surfactant from having too strong irritability to cause scalp inflammation and dandruff, the application explores the oil control ability of different natural substances. It is found that 10-hydroxy decanoic acid derived from royal jelly has oil control and antibacterial effects, can inhibit the activity of Malassezia, and significantly reduce the number of active sebaceous glands, sebum secretion and cumulative sebum secretion, so as to achieve a good oil control effect without reducing the amount of silicone oil or increasing the amount of surfactant.

[0057] Secondly, the scalp of healthy people is also colonized by a large amount of Malassezia without dandruff symptoms, and the application of an equal amount of oleic acid on the scalp of dandruff patients and healthy people will further aggravate the dandruff symptoms of dandruff patients, but the healthy people do not produce dandruff, indicating that in addition to Malassezia itself, the function of the scalp permeability barrier is also an important factor affecting dandruff. The change of the function of the scalp of dandruff people may stimulate the signal pathway of inflammation reaction in the deep skin after the scalp is damaged for a long time (such as excessive cleaning, bleaching, and scratching), thereby causing scalp inflammation. This series of barrier function disorders will cause abnormal differentiation and proliferation of epidermal cells and cause dandruff. In addition, the stimulating metabolites of Malassezia also affect the function of the scalp barrier. When the function of the scalp barrier is damaged, on the one hand, external irritants (including pro-inflammatory unsaturated fatty acids produced by Malassezia) are more likely to penetrate into the stratum corneum, causing inflammation and abnormal dandruff of the scalp; on the other hand, the changes in the scalp pH and the secretion amount of antibacterial peptides caused by the damaged barrier may cause changes in the composition and activity of the scalp microorganisms, promote the colonization of Malassezia. In addition, the decrease in the antioxidant capacity of the scalp of dandruff will also cause scalp inflammation, stimulate the sebaceous glands to secrete more oil, thereby promoting the reproduction of Malassezia and inhibiting the growth of beneficial microorganisms, and thus promoting the generation of dandruff and inflammation.

[0058] Therefore, merely inhibiting Malassezia without repairing the skin barrier function and suppressing scalp inflammation cannot comprehensively and systematically solve the problems of dandruff and scalp oiliness, nor can it fundamentally improve the scalp environment. Consequently, it cannot meet consumers' demands for long-lasting, safe dandruff and oil control. While current biological anti-dandruff peptides have solved the problems of high irritation and easy drug resistance associated with chemical anti-dandruff agents, they still struggle to repair the skin barrier function, suppress scalp inflammation, and control sebum secretion.

[0059] Based on the above problems, this invention has conducted an in-depth study on the influence of the source and preparation method of the effective ingredients from natural sources on their efficacy, with the aim of finding a natural effective ingredient that has significant dandruff removal function, as well as the functions of repairing the skin barrier, inhibiting scalp inflammation and / or controlling sebum secretion.

[0060] Through research, this invention has discovered that sucrose is used as a substrate, and through soil-borne Bacillus (… Paenibacillus edaphicus ) and / or Bacillus subtilis ( Bacillus subtilis The fermentation product of Bacillus subtilis obtained by fermentation contains epidermal repair polysaccharide, which accounts for more than 1% of the total mass. The structure of this epidermal repair polysaccharide is similar to that of mannan, with a molecular weight of 5.8-6.2 MDa. The specific structural formula is shown below:

[0061]

[0062] Tests have shown that the Bacillus fermentation product containing epidermal repair polysaccharides has significant soothing effects, protecting the vitality of HaCat keratinocytes, inhibiting the expression of inflammatory factors, and scavenging free radicals. Furthermore, human efficacy experiments have confirmed that after using shampoos and conditioners containing this Bacillus fermentation product, subjects experienced a significant decrease in dandruff exfoliation index and scalp itching, with no adverse skin reactions observed in any subjects. In addition, the Bacillus fermentation product containing epidermal repair polysaccharides provided by this invention can synergistically enhance the dandruff-removing and oil-controlling abilities of shampoos and conditioners, while maintaining hair smoothness.

[0063] The method for preparing the Bacillus fermentation product includes the following steps:

[0064] (1) For soil-borne Bacillus ( Paenibacillus edaphicus and / or Bacillus subtilis ( Bacillus subtilis) to obtain an activated strain: a sterile inoculation needle is used to take an appropriate amount of glycerol bacteria to streak on a solid culture medium, and cultured at 28℃, 220rpm until single colonies appear. The components of the solid culture medium include 1g / L KH2PO4, 0.1g / L CaCl2, 0.3g / L MgCl2·6H2O, 0.0125g / L FeSO4, 2g / L KNO3, 20g / L sucrose, 10g / L agar, and the components are mixed uniformly, and the pH is adjusted to 7.0-7.2, and then high-temperature high-pressure sterilization (121℃ sterilization for 20min) is performed to obtain the solid culture medium. The source of the Paenibacillus terrae and Bacillus subtilis in the present application is not particularly limited, and a conventional commercially available product can be used.

[0065] (2) Preparation of bacterial seed liquid: single colonies of bacillus in step (1) are picked to LGS medium, and cultured at 28℃, 220rpm for 48h to obtain the bacterial seed liquid. The components of the LGS medium include 10g / L soybean peptone, 5g / L yeast extract, 10g / L glucose, 30g / L sucrose, and the components are mixed uniformly, and the pH is adjusted to 8.0, and then high-temperature high-pressure sterilization (121℃ sterilization for 20min) is performed to obtain the LGS medium.

[0066] (3) Preparation of bacillus fermentation product: the bacterial seed liquid obtained in step (2) is inoculated into a fermentation medium, and the inoculation volume of the bacterial seed liquid is 2% (v / v), and cultured at 28℃, 220rpm for 48h to obtain the bacillus fermentation product. The components of the fermentation medium include 0.003g / L MnSO4·H2O, 0.0125g / L FeSO4·7H2O, 0.0075g / L ZnCl2, 0.07g / L CaCl2, 0.2g / L MgSO4·7H2O, 1.5g / L Na2HPO4, 0.2g / L KNO3, 30g / L sucrose, and the components are mixed uniformly, and the pH is adjusted to 8.0, and then high-temperature high-pressure sterilization (121℃ sterilization for 20min) is performed to obtain the fermentation medium.

[0067] In order to further improve the dandruff removal, oil control, hair smoothing and other effects of the washing and care products, the present application further carries out a variety of parameter experimental operations and verification, and based on the test results, the dandruff control composition as shown in Table 1 is provided, which can be used for preparing shampoo, hair conditioner, hair mask and scalp serum and other washing and care products.

[0068] Table 1 Formulation of the dandruff control composition of the present application

[0069]

[0070] The oil control factor (Finecare oil Control) comprises 10-hydroxy decanoic acid with a mass ratio of 5%, and the anti-dandruff care factor (Finecare BF) comprises 99.5% Bacillus Ferment and 0.5% phenoxyethanol.

[0071] The surfactant comprises but is not limited to sodium fatty alcohol polyoxyethylene ether sulfate (AES), coconut methyl monoethanolamide (CMMEA), lauryl polyether-6 carboxylic acid mixture (AKYPO RLM-6N), and cocamidopropyl betaine (CAB). The conditioning agent in the E phase is emulsified silicone oil, which comprises but is not limited to SILSOFT EM260A emulsified silicone oil and SILSOFT EMU8110-N emulsified silicone oil. The preservative comprises but is not limited to Mikrokill COS.

[0072] Based on the above-mentioned anti-dandruff oil control composition, the present application further provides a preparation method thereof, comprising the following steps:

[0073] S1, uniformly dispersed B phase is added to A phase, stirred and heated to 75-80℃, and kept stirring until each component is completely dissolved.

[0074] S2, the mixed solution prepared in step S1 is cooled to 68-72℃, and each raw material of C phase is added in sequence, and stirred and cooled.

[0075] S3, the mixed solution prepared in step S2 is cooled to 44-46℃, and uniformly dispersed D phase is added, and stirred for 5-15min.

[0076] S4, E phase is added to the mixed solution prepared in step S3, and stirred and cooled.

[0077] S5, the mixed solution prepared in step S4 is cooled to 41-43℃, F phase is added and stirred for 4-10min.

[0078] S6, G phase is added to the mixed solution prepared in step S5, and the pH is adjusted to 5.5-6.5.

[0079] S7, H phase is added to the mixed solution prepared in step S6, and the anti-dandruff oil control composition is prepared.

[0080] The raw materials of A phase comprise 0.01-0.1 parts of chelating agent, 10-20 parts of sodium fatty alcohol polyoxyethylene ether sulfate, 0.04 parts of citric acid monohydrate, and 50-55 parts of water.

[0081] The raw materials of B phase comprise 0.1-0.5 parts of guar gum and 1-5 parts of water, wherein the weight parts of water is 10 times that of guar gum.

[0082] The C-phase raw materials include 1-3 parts of cocoyl methyl monoethanolamide, 2-4 parts of lauryl polyether-6 carboxylic acid mixture, and 2-4 parts of cocamidopropyl betaine.

[0083] The D-phase raw materials include 3-7 parts of suspending agent and 6-14 parts of water, wherein the parts by weight of water is 2 times of the suspending agent.

[0084] The E-phase raw materials include 1.5-4.5 parts of emulsified silicone oil.

[0085] The F-phase raw materials include 0.5-1.5 parts of anti-dandruff care factor and 0.5-1.5 parts of oil control factor.

[0086] The G-phase raw materials include 0.03-0.06 parts of citric acid monohydrate.

[0087] The H-phase raw materials include 0.5-1.5 parts of preservative and 0.5-1.5 parts of sodium chloride.

[0088] The preparation method and efficacy of the anti-dandruff and oil control composition provided by the present application are specifically described below in combination with specific examples.

[0089] Example 1

[0090] The components and their amounts of the anti-dandruff and oil control composition of Example 1 are shown in Table 2.

[0091] The preparation method of the anti-dandruff and oil control composition includes the following steps:

[0092] S1, after the B-phase is uniformly dispersed, it is added into the A-phase, stirred and heated to 75-80℃, and kept stirring until all the components are completely dissolved.

[0093] S2, the mixture prepared in step S1 is cooled to 70℃, and the raw materials of the C-phase are added in sequence, and stirred and cooled.

[0094] S3, the mixture prepared in step S2 is cooled to 45℃, and the uniformly dispersed D-phase is added, and stirred for 10 min; wherein the uniformly dispersed D-phase is prepared by adding 3D cellulose glue into water, and homogenizing for 10 min at 3000 rpm.

[0095] S4, the E-phase is added into the mixture prepared in step S3, and stirred and cooled.

[0096] S5, the mixture prepared in step S4 is cooled to 42℃, the F-phase is added and stirred for 5 min.

[0097] S6, the G-phase is added into the mixture prepared in step S5, and the pH is adjusted to 5.5-6.5.

[0098] S7, the preservative is uniformly mixed in the mixture prepared in step S6, and then the sodium chloride is added to adjust the consistency, and the anti-dandruff and oil control composition is prepared.

[0099] Table 2 Components of the anti-dandruff oil control composition of Example 1 and their amounts

[0100]

[0101] The oil control factor (Finecare oil Control) includes 10-hydroxy decanoic acid with a mass ratio of 5%, and dipropylene glycol with a mass ratio of 95%.

[0102] The anti-dandruff care factor (Finecare BF) includes 99.5% Bacillus fermentation product and 0.5% phenoxyethanol, wherein the Bacillus fermentation product used in Example 1 is a Bacillus fermentation product fermented by Bacillus subtilis var. amyloliquefaciens. The Bacillus fermentation product includes epidermal repair polysaccharide with a mass ratio of 1%, and the molecular weight of the epidermal repair polysaccharide is 5.8-6.2 MDa, and the structural formula is as shown below:

[0103]

[0104] The preparation method of the Bacillus fermentation product includes the following steps:

[0105] (1) Activated culture of Bacillus subtilis var. amyloliquefaciens and / or Bacillus subtilis, to obtain activated strains: use a sterile inoculation needle to take an appropriate amount of glycerol bacteria to streak on a solid culture medium, and culture at 28°C, 220 rpm until single colonies appear.

[0106] (2) Preparation of bacterial seed liquid: pick single colonies of Bacillus subtilis in step (1) into LGS medium, and culture at 28°C, 220 rpm for 48h to obtain a bacterial seed liquid.

[0107] (3) Preparation of Bacillus fermentation product: inoculate the bacterial seed liquid obtained in step (2) into a fermentation medium, and the inoculation volume of the bacterial seed liquid is 2% (v / v), and culture at 28°C, 220 rpm for 48h to obtain a Bacillus fermentation product.

[0108] Comparative Examples 1-3

[0109] The compositions provided in Comparative Examples 1-3 have the same preparation process as the anti-dandruff oil control composition of Example 1, and the components and amounts of each comparative example are shown in Table 3.

[0110] Table 3 Components of the compositions of Comparative Examples 1-3 and their amounts

[0111]

[0112] The anti-dandruff care factor of Comparative Example 2 uses a Bacillus fermentation product fermented by Bacillus subtilis var. amyloliquefaciens.

[0113] Test Example 1

[0114] This test example tests the efficacy of Finecare BF in protecting human keratinocyte HaCat cell viability, inhibiting the expression of inflammatory factors, and scavenging free radicals.

[0115] (1) Test Finecare BF to protect human keratinocyte HaCat cell viability: HaCat cells were seeded in a 96-well plate at a concentration of 1.5 x 10 4 cells / well and cultured for 24 h. The culture medium was removed, washed with FBS, and starved cells were prepared with FBS-free DMEM medium (serum-free medium). The next day, 20 ug / mL of SLS and 1% Finecare BF were used for treatment, and the cells were cultured for 24 h.

[0116] Add 10-fold diluted WST-1 reagent to the culture medium, add 100 uL to each well, and incubate for 2 h. Measure the absorbance OD at 450 nm by ELISA reader (Thermo, 51119300). Calculate the inhibition rate of 1% Finecare BF on SLS-induced HaCat cell death.

[0117] Referring to Figures 1-2 , the fluorescently labeled Finecare BF can bind to the cell membrane of human keratinocyte HaCat cells and protect against the decrease in cell viability caused by SLS (sodium dodecyl sulfate).

[0118] (2) Test the activity of Finecare BF in inhibiting the expression of inflammatory factors: RAW264.7 cells were seeded at a density of 1 x 10 4 After incubation at 37°C for 24 h, 1 ug / mL LPS was added to model the cells, and 1% Finecare BF was added for culture. After 24 h, the supernatant was collected, and the concentrations of TNF-α and IL-6 were determined using MCP-1, TNF-α, and IL-6 enzyme-linked immunoassay kits (purchased from Beijing Xingboseng Biological Technology Co., Ltd.), strictly following the manufacturer's protocol, and measuring OD 450 with a microplate reader and recording.

[0119] Referring to Figures 3-6 , LPS treatment enhances the expression of MCP-1, IL-1β, TNF-ɑ, and IL-6 mRNA in RAW264.7 cells, but Finecare BF treatment significantly inhibits the expression of MCP-1, IL-1β, TNF-ɑ, and IL-6 mRNA.

[0120] (3) Test the activity of Finecare BF in scavenging free radicals, referring to Figure 7The dandruff care factor has good in-vitro antioxidant performance, and the elimination of superoxide anion free radicals, hydroxyl free radicals and DPPH free radicals is in a concentration-dependent manner.

[0121] (4) Test the soothing effect of the dandruff care factor Finecare BF: According to the relevant requirements of GB / T 21808-2008 Chemicals Fish Extended Toxicity 14-Day Test, the soothing effect is evaluated, and the number of neutrophils in the abdomen and tail of zebrafish is analyzed. The model control group solution concentration is 0.16 mg / L of copper sulfate solution.

[0122] Referring to Figure 8 Compared with the model control group, the number of neutrophils in the dandruff care factor group is significantly reduced, indicating that the dandruff care factor has soothing effect.

[0123] Test Example 2

[0124] This test example tests the oil control effect and the effect of reducing skin irritation of the oil control factor: first dilute the oil control factor to 3% with deionized water, and then use it for subsequent tests.

[0125] (1) Short-term oil control effect: 10 cases of healthy volunteers aged 29-50 years old with oily or mixed skin were selected for a double-blind test: 1, 6 hours after the volunteers did not use the sample and after washing their faces with 3% oil control factor or blank sample, the sebum content on the forehead of the volunteers was tested by sebum tape method, and the sebum content when the sample was not washed was set to 0.

[0126] Referring to Figure 9 After using the oil control factor for 1 h, the number of active sebaceous glands and the amount of sebum secretion were significantly reduced; in the 6 h after using the oil control factor, the cumulative increase in sebum secretion was significantly slower than the blank control group.

[0127] (2) Long-term oil control effect: 12 cases of healthy volunteers aged 29-50 years old with oily or mixed skin were selected for a double-blind test of the oil control factor, and the subjects used 3% oil control factor twice a day for 3 weeks.

[0128] Referring to Figure 10 After 3 weeks, the number of active sebaceous glands and the amount of sebum secretion of the subjects were significantly reduced.

[0129] (3) Reducing skin irritation:

[0130] 2.5% sodium dodecyl sulfate (SDS) was used as the irritant source, and dipropylene glycol was used as the control to test the skin irritation of the oil control factor on the isolated abdominal skin.

[0131] (3-1) Prevention: The skin was treated with 3% oil control factor for 24 h, then stimulated with 2.5% SDS, and then transferred to the culture medium. The content of interleukin 1 alpha (IL-1a) in the culture medium was evaluated by ELISA assay.

[0132] (3-2) Repair: The skin was stimulated with 2.5% SDS, then treated with 3% oil control factor for 24 h, and then transferred to the culture medium. The content of IL-1a in the culture medium was evaluated by ELISA assay.

[0133] (3-3) Prevention and repair: The skin was treated with 3% oil control factor for 24 h, then stimulated with 2.5% SDS, and then treated with 3% oil control factor for 24 h. The skin was then transferred to the culture medium. The content of IL-1a in the culture medium was evaluated by ELISA assay.

[0134] Reference Figure 11 Compared with the control group, the oil control factor can significantly reduce the content of IL-1a, proving that the oil control factor has the effect of reducing skin irritation.

[0135] Test Example 3

[0136] 120 cases were selected, aged 18-60 years old, with dandruff, scalp itching, and scalp itching degree score ≥2 points, and healthy male or female subjects with scalp oil content greater than 300. They were randomly divided into 4 groups, 30 people in each group, to test the dandruff and oil control effect of the dandruff and oil control composition of Example 1 and the compositions of Comparative Examples 1-3. The subjects could maintain the regularity of life during the test period, and did not use any cosmetics, drugs and health products that could affect the results. After 14 days of continuous use of the test sample, the changes in dandruff exfoliation index, scalp itching degree and scalp oil content before and after using the test sample were observed by instrument detection, expert visual evaluation (ASFS value), subjective evaluation and evaluation questionnaire method, so as to evaluate the dandruff and oil control efficacy after 14 days of using the test sample, and provide basis for efficacy claim of the test sample.

[0137] (1) Test method

[0138] After the subject's hair was soaked with warm water, an appropriate amount of test sample was evenly applied to the hair, and the scalp was massaged appropriately. After 3-5 minutes, it was washed with clean water.

[0139] (2) Dandruff exfoliation index

[0140] The skin surface texture analysis system Vc20 was used to measure the dandruff exfoliation index of the hair (i.e. the scalp scale exfoliation index). The lower the value, the less dandruff on the scalp.

[0141] Table 4 Dandruff exfoliation index before and after using the test sample

[0142]

[0143] Referring to Table 4, compared with the sample without adding the oil control factor and the anti-dandruff care factor in Comparative Example 1, the dandruff exfoliation of the sample after adding the oil control factor and the anti-dandruff care factor has a very significant decrease (p≤0.001) compared with the base value, wherein the anti-dandruff effect of the anti-dandruff oil control composition of Example 1 with the simultaneous addition of the oil control factor and the anti-dandruff care factor is the best.

[0144] (3) Expert visual perception evaluation ASFS value

[0145] Before using the test sample and on the 14th day of using the test sample, an evaluation and record were made by one expert evaluator, and the evaluation content included: 0: no visible dandruff; 1: occasionally fine and slight dandruff; 2: more dandruff on the scalp and hair; 3: a small amount of large dandruff or a large amount of fine dandruff on the scalp and head; and 4: a large amount of large dandruff.

[0146] Table 5 ASFS value before and after using the test sample

[0147]

[0148] Referring to Table 5, after using the four test samples for 14 days, the expert visual perception evaluation (ASFS value) of Example 1, Comparative Example 2 and Comparative Example 3 all significantly decreased compared with the base value, wherein the sample of Example 1 significantly decreased (p≤0.001) compared with the base value, and the expert visual perception evaluation (ASFS value) of Example 1 was the lowest in the four groups.

[0149] (4) Degree of scalp itching rating

[0150] Before using the test sample and on the 14th day of using the test sample, an evaluation and record were made by one expert evaluator, and the evaluation content included: 1 point (0 degree): basically no itching; 2 points (mild, degree I): slightly itchy, can not scratch, and can be relieved by lightly patting, and no secondary rash caused by scratching; 3 points (moderate, degree II): obvious itching, cannot help scratching, and mild secondary rash caused by scratching can be seen; 4 points (severe, degree III): severe itching, affecting sleep and work, and obvious secondary rash caused by scratching can be seen on the local part; and 5 points (extremely severe, degree IV): severe itching, affecting sleep and work, emotional instability, and heavy secondary rash.

[0151] Table 6 Degree of scalp itching rating before and after using the test sample

[0152]

[0153] Referring to Table 6, the degree of scalp itch rating of the dandruff control and oil control composition of Example 1 after 14 days of use was significantly decreased (p≤0.001) compared to the base value, and the average value of the degree of scalp itch rating of the test area of Example 1 was the lowest among the four groups.

[0154] (5) Scalp oil content

[0155] The scalp oil content was measured using a scalp oil tester MB560, and a higher value indicates a higher scalp oil content.

[0156] Table 7 Scalp oil content test results

[0157]

[0158] Referring to Table 7, the scalp oil content of the test sample area after 2h and 8h of single use of the four test samples was significantly decreased compared to the base value, and the scalp oil content of the test area of Example 1 was significantly decreased (p≤0.001) compared to the base value, and the scalp oil content of the test area of Example 1 was the lowest among the four groups.

[0159] (6) Evaluation of the use of the dandruff control and oil control composition of Example 4 by the subjects

[0160] The subjects (30 test subjects) rated the test samples according to the evaluation criteria shown in Table 8 after 2h, 8h, and 14 days, wherein the rating criteria for each evaluation criterion were: 1 point for "very dissatisfied", 2 points for "dissatisfied", 3 points for "average", 4 points for "satisfied", and 5 points for "very satisfied", and the proportion of the number of people who scored 4 points or more was calculated.

[0161] Table 8 Evaluation of the use by the subjects

[0162]

[0163] (7) Skin adverse reaction test

[0164] According to the skin adverse reaction grading standard for human use tests in the 2015 edition of "Cosmetic Safety Technology Specifications", a 14-day human use test was conducted on 120 subjects.

[0165] Table 9 Skin adverse reaction test of the subjects on the four test samples

[0166]

[0167] Referring to Table 9, no skin adverse reactions were observed in any of the subjects.

[0168] Referring to Figure 12 , which is the dandruff control effect of the dandruff control and oil control composition of Example 1.

[0169] In summary, the dandruff control and oil control composition provided by the present application has the following advantages:

[0170] (1) Unlike traditional chemical dandruff removers, the fermentation product has little irritation but strong dandruff removal ability. It can effectively replace chemical dandruff removers, avoid the problem of Malassezia developing drug resistance caused by the use of chemical dandruff removers, reduce skin irritation, provide a more gentle and long-lasting dandruff removal solution for consumers, and achieve long-term stable dandruff removal effect.

[0171] (2) The Bacillus fermentation product also has multiple functions beneficial to the health of the scalp. It can repair damaged skin barrier and enhance the self-protection ability of the scalp; inhibit the occurrence of scalp inflammation and relieve the discomfort symptoms such as itching and redness caused by inflammation; clear free radicals and reduce the damage of free radicals to scalp cells, thereby significantly improving the overall environment of the scalp and creating good conditions for the healthy growth of hair, further consolidating the dandruff removal effect.

[0172] (3) The oil control factor provided by the present application can precisely act on the sebaceous glands, significantly reduce the number of active sebaceous glands, and reduce the secretion of sebum from the source. At the same time, it can also effectively reduce the amount of sebum secretion and cumulative sebum secretion, and exhibit good oil control effect whether used for a short or long time, helping consumers to keep the scalp clean and reduce the problems of greasy and flat hair caused by excessive oil.

[0173] (4) The oil control factor also has antibacterial activity and can inhibit the activity of Malassezia. Malassezia is one of the important factors causing dandruff, and by inhibiting its activity, it can assist dandruff removal from another angle. In addition, the oil control factor can also reduce skin irritation and further reduce the adverse effects on the scalp, so that consumers can experience a more comfortable and gentle experience during use.

[0174] (5) Compared with the use of oil control factor alone and dandruff maintenance factor alone, the simultaneous use of oil control factor and dandruff maintenance factor can significantly improve the dandruff control and oil control ability of the dandruff control and oil control composition, indicating that the oil control factor and the Bacillus fermentation product containing epidermal repair polysaccharide jointly act from multiple angles to repair the skin barrier, inhibit scalp inflammation, clear free radicals, inhibit Malassezia, inhibit sebum secretion, etc., forming a complete dandruff control and oil control system. They complement and promote each other, comprehensively improve the dandruff control and oil control ability of the washing and caring product, and provide consumers with a more efficient and comprehensive scalp care solution.

[0175] The above embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the patent. It should be noted that for ordinary skilled in the art, without departing from the concept of the present application, several modifications and improvements can be made, which are within the scope of protection of the present application.

Claims

1. An anti-dandruff oil control composition characterized in that, The composition is composed of the following components in parts by weight: 0.5-1.5 parts of anti-dandruff care factor, 0.5-1.5 parts of oil control factor, 0.1-0.5 parts of guar gum, 1.5-4.5 parts of emulsified silicone oil, 0.5-1.5 parts of sodium chloride, 15-31 parts of surfactant, 3-7 parts of suspending agent, 0.5-1.5 parts of preservative, 0.01-0.1 parts of chelating agent, and 56-74 parts of water; The oil control factor includes 5% of 10-hydroxydecanoic acid by mass ratio. The anti-dandruff care factor comprises 99.5% Bacillus fermentation product and 0.5% phenoxyethanol. The Bacillus fermentation product uses sucrose as a substrate and is processed by soil-borne Bacillus (…). Paenibacillus edaphicus ) and / or Bacillus subtilis ( Bacillus subtilis It is obtained through fermentation; The Bacillus fermentation product includes 0.5-1.5% of epidermis repair polysaccharide by mass ratio, and the molecular weight of the epidermis repair polysaccharide is 5.8-6.2 MDa, and the structural formula is as follows: ； The preparation method of the Bacillus fermentation product includes the following steps: (1) activating culture of Paenibacillus terrae and / or Bacillus subtilis to obtain activated strains; (2) picking single colonies of the activated strains of step (1) into LGS medium and culturing at 25-30°C for 36-60h to obtain a seed liquid of the strains; (3) preparing a fermentation medium with sucrose as a substrate, inoculating the seed liquid of the strains obtained in step (2) into the fermentation medium, and culturing at 25-30°C for 36-60h to obtain the Bacillus fermentation product.

2. The anti-dandruff oil control composition according to claim 1, characterized in that: The inoculation volume of the seed liquid of the strains is 1-3% (v / v).

3. The anti-dandruff oil control composition according to claim 1, wherein The surfactant includes one or more of sodium fatty alcohol polyoxyethylene ether sulfate, cocomethyl monoethanolamide, lauryl polyoxyethylene-6 carboxylic acid mixture, and cocamidopropyl betaine.

4. The anti-dandruff oil control composition according to claim 3, characterized in that: The surfactant is sodium fatty alcohol polyoxyethylene ether sulfate, cocomethyl monoethanolamide, lauryl polyoxyethylene-6 carboxylic acid mixture, and cocamidopropyl betaine, and the parts by weight of each are 10-20 parts, 1-3 parts, 2-4 parts, and 2-4 parts, respectively.

5. The anti-dandruff oil control composition according to claim 1, wherein The preservative includes Mikrokill COS.

6. Use of the anti-dandruff and oil control composition of any one of claims 1-5 in the preparation of a hair care product.

7. Use according to claim 6, characterized in that: The hair care product includes shampoo, hair conditioner, hair mask, and scalp serum.

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