Traditional Chinese medicine slow-release bar for treating postpartum blood stasis of cattle and horses in animal husbandry and preparation method of traditional Chinese medicine slow-release bar

Abstract

The invention provides a traditional Chinese medicine slow-release bar for treating postpartum blood stasis of cattle and horses in animal husbandry and a preparation method thereof. The slow-release bar comprises a traditional Chinese medicine extract and a slow-release adjusting matrix. The slow-release adjusting matrix comprises a hydrophilic matrix, a hydrophobic matrix and a matrix with an amphiphilic structure, and gradient release is formed through hydrophilic-hydrophobic material combination. The three components jointly construct a'physical retardation-molecular solubilization-dynamic release 'multistage sustained-release system, so that the half-life period of the drug is remarkably prolonged, and the drug administration frequency is reduced. According to the product process, a multi-layer structure of a controlled release layer, a medicine core layer and a supporting layer of a conventional rod agent is simplified, and the production efficiency is improved while the production cost is reduced.

Description

Technical Field

[0001] This invention relates to the field of veterinary drug sustained-release rod preparation technology, and in particular to a traditional Chinese medicine sustained-release rod for treating postpartum blood stasis in cattle and horses and its preparation method. Background Technology

[0002] The limitations of existing veterinary drugs for treating postpartum hemorrhage in cattle and horses are as follows: Chemical drugs and ordinary traditional Chinese medicine sticks require daily injections or oral administration. Multiple administrations not only increase the workload of farmers but also cause stress in animals due to repeated procedures, affecting postpartum recovery. Traditional Chinese medicine powders disperse quickly in water, resulting in uneven drug release and easy excretion of active ingredients, requiring frequent administration to maintain efficacy. Furthermore, absorption rates are significantly affected by the gastrointestinal environment. Chemically synthesized sustained-release agents may leave toxic residues, and long-term use can lead to drug accumulation, threatening animal health and the safety of livestock products.

[0003] Traditional Chinese medicine (TCM) sustained-release sticks prolong therapeutic effects through sustained-release and controlled-release technology. By encapsulating the active ingredients of TCM with polymer materials, they achieve slow drug release, maintain effective blood drug concentrations, and reduce the frequency of administration. TCM extracts are used to increase the dissolution rate of active ingredients. Biocompatible materials are selected as drug excipients to enhance drug absorption and utilization. By integrating the advantages of traditional Chinese medicine compound formulas with modern sustained-release technology, TCM sustained-release sticks address the shortcomings of existing veterinary drugs in terms of administration frequency, bioavailability, and safety. They provide a highly efficient, low-toxicity, and environmentally friendly solution for treating postpartum hemorrhage in cattle and horses, and are of great significance for promoting precision animal medicine and green farming. Summary of the Invention

[0004] To overcome the shortcomings of the prior art, this invention discloses a traditional Chinese medicine sustained-release stick for treating postpartum blood stasis in cattle and horses, and its preparation method.

[0005] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: A traditional Chinese medicine sustained-release stick for treating postpartum blood stasis in cattle and horses comprises a traditional Chinese medicine extract and a sustained-release regulating matrix, together forming a 160-200g / stick sustained-release stick, by weight: Chinese herbal extracts: Safflower extract (concentration ratio 10:1) 6-12 parts, Peach kernel extract (concentration ratio 10:1) 10-15 parts, Typha pollen extract (concentration ratio 30:1) 4-8 parts, Dipsacus extract (concentration ratio 20:1) 6-12 parts, Lindera extract (concentration ratio 30:1) 4-8 parts, Sparganium extract (concentration ratio 30:1) 2-5 parts, Honeysuckle extract (concentration ratio 30:1) 2-8 parts, Astragalus extract (concentration ratio 30:1) 6-12 parts, Artemisia argyi extract (concentration ratio 30:1) 1-4 parts, Corydalis extract (concentration ratio 20:1) 2-8 parts, White peony extract (concentration ratio 20:1) 2-4 parts.

[0006] Slow-release matrix: 25-35 parts of hydrophilic matrix, 85-95 parts of hydrophobic matrix, and 15-25 parts of matrix with amphiphilic structure.

[0007] The preparation method of the above-mentioned sustained-release herbal sticks is as follows: (1) Weigh each slow-release regulating matrix according to the above proportions, mix them, and obtain mixture A; (2) Weigh each Chinese herbal extract according to the above proportions, mix them, and obtain mixture B; (3) Heat mixture A until it melts and stir until it becomes a liquid; (4) Add mixture B, mix evenly, and hot melt injection molding to obtain a Chinese medicine sustained-release stick for treating postpartum blood stasis in cattle and horses.

[0008] Furthermore, the hydrophilic matrix is ​​at least one of chitosan, hyaluronic acid, polyethylene glycol 4000, sodium alginate, poly(N-isopropylacrylamide), polyoxyethylene, and polyethylene glycol-chitosan copolymer.

[0009] Furthermore, the hydrophobic matrix is ​​at least one of the following: hydrophobic matrix acrylate copolymer, calcium stearate, paraffin emulsion, hydrogenated vegetable oil, polyoxyethylene monostearate, silica, beeswax, and octadecyltrimethylammonium bromide.

[0010] Furthermore, the amphiphilic matrix is ​​at least one of poloxamer 188, polysorbate 80, polycaprolactone, and polyethylene glycol palmitic acid.

[0011] Furthermore, in steps (3) and (4), the temperature of the heating and melting of the hot-melt column mold is 75-85°C, and the condensation time is 5-10h.

[0012] Furthermore, the size of the Chinese medicine sustained-release stick described in step (4) is adapted to the feeder / rumen retention device.

[0013] In the drug described in this invention: safflower Meridians: Heart Meridian, Liver Meridian Properties: pungent, warm Functions and indications: Promotes blood circulation and regulates menstruation, disperses blood stasis and relieves pain. It is mainly used to treat amenorrhea due to blood stasis, dysmenorrhea, postpartum abdominal pain due to blood stasis, abdominal masses, chest pain, traumatic injuries, and swelling and pain due to blood stasis.

[0014] Pharmacological studies: It contains safflower yellow pigment, safflower quinone glycoside, etc., and has anticoagulant, antiplatelet aggregation, anti-inflammatory and antioxidant effects, and can improve microcirculation.

[0015] Related discussions: The Compendium of Materia Medica states: "It invigorates blood circulation, moistens dryness, relieves pain, reduces swelling, and regulates menstruation." The Compendium of Materia Medica states: "Safflower is a medicine that breaks up blood stasis, promotes blood circulation, harmonizes blood, and regulates blood." peach kernel Meridians involved: Heart Meridian, Liver Meridian, Large Intestine Meridian Taste and properties: bitter, sweet, neutral Functions and indications: Promotes blood circulation and removes blood stasis, moistens the intestines and relieves constipation. It is mainly used to treat various symptoms caused by blood stasis (such as amenorrhea, dysmenorrhea, abdominal masses), lung abscess, intestinal abscess, traumatic injuries, and constipation due to intestinal dryness.

[0016] Pharmacological studies: It contains amygdalin and peach kernel oil, which have antithrombotic, anti-inflammatory, and analgesic effects. Its laxative effect is related to its oil composition.

[0017] Related discussions: The Shennong Materia Medica states: "It treats blood stasis, amenorrhea, abdominal masses, pathogenic factors, and kills small insects." According to the "Medicinal Properties of Peach Kernel", "Peach kernel is bitter and can purge blood heat, and its moist texture can nourish dry intestines." Astragalus Meridians: Spleen Meridian, Lung Meridian Properties: Sweet, slightly warm Functions and indications: Tonifies Qi and raises Yang, strengthens Wei Qi and consolidates the exterior, promotes tissue regeneration and detoxification, and promotes diuresis and reduces edema. It is indicated for spleen Qi deficiency, lung Qi deficiency, spontaneous sweating due to Qi deficiency, Qi and blood deficiency, sores that are difficult to heal or have been ulcerated for a long time, and edema.

[0018] Pharmacological studies: Contains astragalus polysaccharides and flavonoids, which can enhance immunity, combat fatigue, regulate blood pressure, and protect the cardiovascular system.

[0019] Related discussions: The Compendium of Materia Medica states: "Astragalus is a medicine that nourishes the lungs and strengthens the spleen, strengthens the defensive qi and stops sweating, and dispels wind and toxins." The Medical Origins states: "Astragalus has five uses: first, to replenish all deficiencies; second, to benefit the original qi; third, to strengthen the spleen and stomach; fourth, to remove heat from the muscles; fifth, to drain pus and relieve pain, to invigorate blood and generate blood, and to internally support yin carbuncles, making it a holy medicine for sores." White Peony Root Meridians: Liver Meridian, Spleen Meridian Taste and properties: bitter, sour, slightly cold Functions and indications: Nourishes blood and regulates menstruation, astringes yin and stops sweating, softens the liver and relieves pain, and calms liver yang. It is mainly used to treat blood deficiency and chlorosis, irregular menstruation, spontaneous sweating and night sweats, hypochondriac pain and abdominal pain, limb spasms and pain, and headache and dizziness due to liver yang hyperactivity.

[0020] Pharmacological studies: Contains paeoniflorin and volatile oil, and has analgesic, sedative, anti-inflammatory, immunomodulatory, and hepatoprotective effects.

[0021] Related discussions: The Medical Origins states: "White peony root has a slightly cold nature and a sour and bitter taste. It can replenish the middle qi, treat abdominal pain due to blood deficiency, and relieve fatigue and fever." The Compendium of Materia Medica states: "White peony benefits the spleen and can drain wood from the earth." honeysuckle Meridians involved: Lung Meridian, Stomach Meridian, Large Intestine Meridian Properties: Sweet, cold Functions and indications: Clears heat and detoxifies, disperses wind-heat. Used to treat carbuncles, boils, sore throat, erysipelas, dysentery due to heat toxicity, wind-heat colds, and fever caused by febrile diseases.

[0022] Pharmacological studies: Contains chlorogenic acid and luteolin, and has broad-spectrum antibacterial, antiviral, anti-inflammatory, and antipyretic effects.

[0023] Related discussions: The Compendium of Materia Medica states: "It treats all kinds of rheumatism, swellings, boils, scabies, syphilis, and other malignant sores, clearing heat and detoxifying." The Compendium of Materia Medica states: "It is used to treat heat toxins, bloody dysentery, and watery dysentery." mugwort Meridians involved: Liver, Spleen, Kidney Properties: pungent, bitter, warm Functions and indications: Warms the meridians and stops bleeding, dispels cold and relieves pain, eliminates dampness and relieves itching. It is mainly used to treat bleeding due to deficiency and cold (metrorrhagia, menorrhagia), threatened abortion, cold pain in the epigastrium and abdomen, irregular menstruation due to cold, and eczema with itching.

[0024] Pharmacological studies: Contains volatile oils (eucalyptol, camphor), and has anti-inflammatory, antibacterial, uterine contraction-regulating, and anti-allergic effects.

[0025] Related discussions: The Compendium of Materia Medica states: "When raw, mugwort leaves are slightly bitter and very pungent; when cooked, they are slightly pungent and very bitter. Raw mugwort is warm, and cooked mugwort is hot; it is pure yang. It can draw upon the true fire of the sun and can revive depleted primordial yang." The Treatise on the Properties of Medicinal Herbs states: "It stops uterine bleeding, calms the fetus, and relieves abdominal pain." Corydalis Meridians: Liver Meridian, Spleen Meridian Properties: pungent, bitter, warm Functions and indications: Promotes blood circulation, regulates qi, and relieves pain. It is mainly used to treat various pains caused by qi and blood stagnation (chest, rib, and abdominal pain, amenorrhea and dysmenorrhea, postpartum blood stasis, and swelling and pain from falls).

[0026] Pharmacological studies: Contains corydaline (levotetrahydropalmatine), which has analgesic, sedative, and central nervous system depressant effects.

[0027] Related discussions: The Compendium of Materia Medica states: "Corydalis can move the blood and qi stagnation, and thus it is specifically used to treat various pains throughout the body." The Treatise on Processing Medicinal Herbs by Lei Gong states: "If you feel excruciating heart pain, quickly seek Corydalis rhizome." Lindera Meridians involved: Lung Meridian, Spleen Meridian, Kidney Meridian, Bladder Meridian Properties: pungent, warm Functions and indications: Promotes qi circulation and relieves pain, warms the kidneys and dispels cold. It is mainly used to treat various chest and abdominal pains caused by cold stagnation and qi stagnation (abdominal distension and pain, belching and acid reflux), abdominal pain due to cold hernia, abdominal pain due to cold during menstruation, frequent urination and urinary incontinence.

[0028] Pharmacological studies: It contains lindera lactone and lindera alkyl, and has the effects of regulating gastrointestinal function, anti-inflammatory and analgesic.

[0029] Related discussions: The Compendium of Materia Medica states: "Lindera has a strong and warm nature, so it can quickly promote the flow of qi and disperse stagnation." "Ben Cao Tong Xuan" states: "It treats the stagnation of the seven emotions and the stagnation of qi and blood." Intermittent Meridians involved: Liver Meridian, Kidney Meridian Properties: Bitter, pungent, slightly warm Functions and indications: Tonifies the liver and kidneys, strengthens tendons and bones, heals fractures, and stops metrorrhagia. Primarily used to treat lower back and knee pain, rheumatic pain, injuries from falls, tendon injuries and fractures, and excessive menstrual bleeding due to liver and kidney deficiency.

[0030] Pharmacological studies: It contains Dipsacus saponins and volatile oils, and has anti-osteoporosis, bone healing promotion and anti-inflammatory effects.

[0031] Related discussions: The Compendium of Materia Medica states: "Dipsacus asper is an essential medicine for tonifying the liver and kidneys, strengthening the Chong and Ren meridians, and repairing tendons and bones." The Yunnan Materia Medica states: "It nourishes the liver and kidneys, strengthens muscles and bones, and stops metrorrhagia and menorrhagia." triangular Meridians: Liver Meridian, Spleen Meridian Taste and properties: bitter, neutral Functions and indications: It breaks up blood stasis, promotes qi circulation, eliminates stagnation, and relieves pain. It is mainly used to treat abdominal masses, dysmenorrhea, amenorrhea, chest pain, abdominal distension due to food stagnation, and injuries from falls.

[0032] Pharmacological studies: Contains volatile oil and phenylethanol glycosides, and has anti-platelet aggregation, anti-tumor, and anti-inflammatory effects.

[0033] Related discussions: The Compendium of Materia Medica states: "Sparganium rhizome can break up stagnation and dissipate lumps, thus it can treat various diseases." The Rihua Materia Medica states: "It treats women's blood disorders and abdominal pain." Typhae Pollen Meridians: Liver Meridian, Pericardium Meridian Properties: Sweet, neutral Indications: Hemostasis, blood stasis removal, and urination relief. Mainly used to treat various types of bleeding (hemoptysis, epistaxis, metrorrhagia, traumatic bleeding), dysmenorrhea due to blood stasis, swelling and pain from falls, and painful urination with blood in the urine.

[0034] Pharmacological studies: Contains typhain and quercetin, and has anti-platelet aggregation, anti-inflammatory, and diuretic effects.

[0035] Related discussions: The Compendium of Materia Medica states: "Typha pollen is a medicine for the blood of the Jueyin meridian of the hands and feet, so it can treat blood stasis and pain." The Illustrated Materia Medica states: "It treats cold and heat in the heart, abdomen, and bladder, promotes urination, stops bleeding, and eliminates blood stasis." Licorice Meridians involved: Heart Meridian, Lung Meridian, Spleen Meridian, Stomach Meridian Properties: Sweet, neutral Functions and indications: Tonifies the spleen and replenishes qi, clears heat and detoxifies, eliminates phlegm and relieves cough, relieves spasms and pain, and harmonizes the effects of other medicines. It is indicated for spleen and stomach weakness, fatigue, palpitations, shortness of breath, cough with excessive phlegm, abdominal and limb spasms and pain, carbuncles and sores caused by heat toxins, and sore throat.

[0036] Pharmacological studies: Contains glycyrrhizic acid and flavonoids, and has anti-inflammatory, anti-allergic, immunomodulatory, and detoxifying effects.

[0037] Related discussions: The Compendium of Materia Medica states: "Licorice has an extremely sweet taste, possesses a neutral nature, and has the function of regulating and nourishing." The "Pharmacological Methods of Using Herbs" states: "Licorice is used to supplement those with insufficient Yang, as its sweetness and warmth can dispel excessive heat." Compared with the prior art, the present invention has the following beneficial effects: (1) Sustained-release synergy, matrix swelling-diffusion: The sustained-release regulating matrix comprises a hydrophilic matrix, a hydrophobic matrix, and a matrix with an amphiphilic structure, forming a gradient release through the combination of hydrophilic and hydrophobic materials. The hydrophilic matrix softens the matrix by fixing water molecules with hydrogen bonds; its high water solubility and biocompatibility ensure stable drug release without irritation. The hydrophobic matrix forms a sustained-release barrier through hydrophobic encapsulation, and its plasticity regulates the drug diffusion rate, making it suitable for long-acting rod development. The amphiphilic matrix enhances the absorption of poorly soluble drugs through micellar solubilization, while also acting as an emulsion stabilizer to maintain rod homogeneity. Together, these three components construct a multi-stage sustained-release system of "physical retardation-molecular solubilization-dynamic release," significantly extending drug half-life and reducing dosing frequency. This product's process simplifies the multi-layered structure of conventional rod formulations, consisting of a controlled-release layer, a drug core layer, and a support layer, reducing production costs while improving production efficiency.

[0038] (2) Enhanced efficacy of the formulation, and the combination of principal, assistant, and adjuvant herbs reduces drug resistance in target animals: Safflower and peach kernel are the principal herbs: safflower promotes menstruation and removes blood stasis, while peach kernel breaks up blood stasis and removes blood stasis. They work together to enhance the blood-activating and stasis-removing effects, targeting the main symptoms of postpartum uterine blood stasis and lochia retention. Honeysuckle and cattail pollen are the assistant herbs: Honeysuckle clears heat and detoxifies, preventing blood stasis from turning into heat; cattail pollen has the function of promoting blood circulation and stopping bleeding, assisting the principal herb in clearing blood stasis while avoiding excessive bleeding. Together, they are the assistant herbs, harmonizing the relationship between clearing heat and removing blood stasis. Astragalus, Artemisia argyi, Corydalis yanhusuo, Paeonia lactiflora, Dipsacus asper, and Lindera strychnifolia are used as adjuvant herbs: Astragalus tonifies Qi and raises Yang, enhances uterine contraction, and promotes lochia discharge; Artemisia argyi warms the meridians and dispels cold, improving blood stasis caused by cold; Corydalis yanhusuo promotes Qi circulation and relieves pain, alleviating abdominal pain; Paeonia lactiflora softens the liver and nourishes blood, harmonizing Qi and blood; Dipsacus asper tonifies the liver and kidneys, strengthens tendons and bones, and promotes uterine repair; Lindera strychnifolia promotes Qi circulation and relieves pain, while Sparganium stoloniferum breaks up blood stasis and unblocks the meridians. These herbs assist the principal and assistant herbs, simultaneously tonifying Qi and blood, and warming and unblocking the meridians. They can protect postpartum weakness and dispel cold pathogens from the uterus. Licorice is used as an adjuvant to harmonize the properties of other herbs, alleviate their urgency and regulate the middle jiao (digestive system), reduce the stimulation of blood-activating herbs on the spleen and stomach, and at the same time replenish the middle jiao and boost qi, thus enhancing the overall tonifying effect of the formula.

[0039] The pharmacological advantages of this invention's sustained-release herbal stick in treating postpartum blood stasis in dairy cows lie in the synergistic effects of multiple components and the network-based regulation of targets. Safflower yellow pigment from safflower and amygdalin from peach kernel synergistically inhibit platelet aggregation and improve uterine microcirculation; Astragalus polysaccharides and paeoniflorin jointly regulate T-cell activity, balancing postpartum immunosuppression; chlorogenic acid from honeysuckle and quercetin from typhae pollen form a dual antibacterial-anti-inflammatory barrier, inhibiting biofilm formation by pathogens such as Staphylococcus aureus. Corydalis yanhusuo and lindera lactone alleviate uterine spasmodic pain by regulating 5-HT receptors; Dipsacus saponins promote endometrial repair; and the Curcuma zedoaria complex activates the fibrinolytic system, accelerating the decomposition of placental remnants. Compared to traditional classic prescriptions (such as Taohong Siwu Decoction or Sheng Hua Decoction), this formula introduces Artemisia argyi to warm the meridians and stop bleeding, reducing the risk of postpartum hemorrhage. Glycyrrhizic acid chelates metal ions to enhance detoxification effects. At the same time, the sustained-release process improves the utilization rate of medicinal materials, increasing the bioavailability of effective components by more than 30%. Furthermore, it reduces the dose-dependent toxicity of single components through multi-target linkage.

[0040] (3) Veterinary compatibility: This invention relates to a herbal sustained-release stick, sized (160-200g, 3.2cm in diameter, 14cm in length) to fit a feeder or rumen retention device. The rumen retention device precisely anchors the herbal sustained-release stick within the rumen, preventing regurgitation or premature emptying of the rumen. Compared to traditional oral administration, the drug's retention time in the rumen is extended by 3-5 times, reducing the frequency of repeated administration, effectively minimizing stress in dairy cows, and improving treatment compliance. A single implantation replaces the traditional twice-daily injections, reducing labor costs by 60%.

[0041] The combination of a slow-release herbal medicine stick with a feeder / rumen retention device solves the problems of traditional veterinary drugs, such as short efficacy, high stress, and high residue. It effectively improves treatment outcomes, reduces labor intensity, and ensures food safety, providing a revolutionary solution for precision animal husbandry and green farming. Detailed Implementation

[0042] The present invention will be further described below with reference to specific embodiments. The following are only specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Example 1

[0043] A traditional Chinese medicine sustained-release stick for treating postpartum blood stasis in cattle and horses comprises a traditional Chinese medicine extract and a sustained-release regulating matrix, together forming a 160-200g / stick sustained-release stick, by weight: Chinese herbal extracts: 9 parts safflower extract (concentration ratio 10:1), 12 parts peach kernel extract (concentration ratio 10:1), 6 parts cattail pollen extract (concentration ratio 30:1), 9 parts dipsacus extract (concentration ratio 20:1), 6 parts lindera extract (concentration ratio 30:1), 3 parts sparganium extract (concentration ratio 30:1), 5 parts honeysuckle extract (concentration ratio 30:1), 9 parts astragalus extract (concentration ratio 30:1), 2 parts artemisia argyi extract (concentration ratio 30:1), 5 parts corydalis extract (concentration ratio 20:1), and 3 parts white peony extract (concentration ratio 20:1).

[0044] Slow-release matrix: 30 parts poly-N-isopropylacrylamide, 90 parts beeswax, and 20 parts polyethylene glycol palmitic acid.

[0045] The preparation method of the above-mentioned sustained-release herbal sticks is as follows: (1) Weigh each slow-release regulating matrix according to the above proportions, mix them, and obtain mixture A; (2) Weigh each Chinese herbal extract according to the above proportions, mix them, and obtain mixture B; (3) Heat mixture A to 80°C until melted, stir until homogeneous, and obtain a liquid state; (4) Add mixture B, mix evenly, hot melt injection molding at 80℃, and condense for 8 hours to obtain a Chinese medicine sustained-release stick for treating postpartum blood stasis in cattle and horses. Example 2

[0046] A traditional Chinese medicine sustained-release stick for treating postpartum blood stasis in cattle and horses comprises a traditional Chinese medicine extract and a sustained-release regulating matrix, together forming a 160-200g / stick sustained-release stick, by weight: Chinese herbal extracts: 10 parts safflower extract (concentration ratio 10:1), 13 parts peach kernel extract (concentration ratio 10:1), 7 parts cattail pollen extract (concentration ratio 30:1), 10 parts dipsacus extract (concentration ratio 20:1), 7 parts lindera extract (concentration ratio 30:1), 4 parts sparganium extract (concentration ratio 30:1), 7 parts honeysuckle extract (concentration ratio 30:1), 10 parts astragalus extract (concentration ratio 30:1), 3 parts artemisia argyi extract (concentration ratio 30:1), 7 parts corydalis extract (concentration ratio 20:1), and 3 parts white peony extract (concentration ratio 20:1).

[0047] Sustained-release matrix: 22 parts sodium alginate, 92 parts calcium stearate, and 18 parts poloxamer 188.

[0048] The preparation method of the above-mentioned sustained-release herbal sticks is as follows: (1) Weigh each slow-release regulating matrix according to the above proportions, mix them, and obtain mixture A; (2) Weigh each Chinese herbal extract according to the above proportions, mix them, and obtain mixture B; (3) Heat mixture A to 78°C until melted, stir until homogeneous, and obtain a liquid state; (4) Add mixture B, mix evenly, hot melt injection molding at 85℃, and condense for 9 hours to obtain a Chinese medicine sustained-release stick for treating postpartum blood stasis in cattle and horses. Example 3

[0049] A traditional Chinese medicine sustained-release stick for treating postpartum blood stasis in cattle and horses comprises a traditional Chinese medicine extract and a sustained-release regulating matrix, together forming a 160-200g / stick sustained-release stick, by weight: Chinese herbal extracts: 7 parts safflower extract (concentration ratio 10:1), 11 parts peach kernel extract (concentration ratio 10:1), 5 parts cattail pollen extract (concentration ratio 30:1), 7 parts dipsacus extract (concentration ratio 20:1), 5 parts lindera extract (concentration ratio 30:1), 2 parts sparganium extract (concentration ratio 30:1), 3 parts honeysuckle extract (concentration ratio 30:1), 7 parts astragalus extract (concentration ratio 30:1), 2 parts artemisia argyi extract (concentration ratio 30:1), 3 parts corydalis extract (concentration ratio 20:1), and 3 parts white peony extract (concentration ratio 20:1).

[0050] Slow-release matrix: 28 parts polyethylene glycol-chitosan copolymer, 86 parts acrylate copolymer and 8016 parts polysorbate.

[0051] The preparation method of the above-mentioned sustained-release herbal sticks is as follows: (1) Weigh each slow-release regulating matrix according to the above proportions, mix them, and obtain mixture A; (2) Weigh each Chinese herbal extract according to the above proportions, mix them, and obtain mixture B; (3) Heat mixture A to 82°C until melted, stir until homogeneous, and obtain a liquid state; (4) Add mixture B, mix evenly, hot melt injection molding at 82℃, and condense for 6 hours to obtain a Chinese medicine sustained-release stick for treating postpartum blood stasis in cattle and horses.

[0052] Comparative Example 1 Compared to Example 1, 2 parts of safflower extract (concentration ratio 10:1) were used, and no hydrophilic matrix was added; the rest were the same.

[0053] Comparative Example 2 Compared to Example 1, one part of Typhae pollen extract (concentration ratio 30:1) was used, and no hydrophobic matrix was added; the rest were the same.

[0054] Comparative Example 3 Compared to Example 1, two parts of Astragalus extract (concentration ratio 30:1) were used, and no matrix with an amphiphilic structure was added; the rest were the same.

[0055] Experimental example: Treatment efficacy trial.

[0056] A relatively large dairy farm with 10,000 cows was selected. Seventy cows with similar age, parity, body condition, and expected calving date who had postpartum lochia retention and blood stasis abdominal pain were randomly divided into seven groups (experimental group 1, experimental group 2, experimental group 3, control group 1, control group 2, control group 3, and blank group), with 10 cows in each group.

[0057] Experimental Group 1: Each cow was given the Chinese medicine sustained-release stick prepared in Example 1 once a week for 8 consecutive weeks.

[0058] Experimental Group 2: Each cow was given the Chinese medicine sustained-release stick prepared in Example 2 once a week for 8 consecutive weeks.

[0059] Experimental Group 3: Each cow was given the Chinese medicine sustained-release stick prepared in Example 3 once a week for 8 consecutive weeks.

[0060] Control group 1: Each cow was given the Chinese medicine sustained-release stick prepared in control group 1 once a week for 8 consecutive weeks.

[0061] Control group 2: Each cow was given the Chinese medicine sustained-release stick prepared in control group 2 once a week for 8 consecutive weeks.

[0062] Control group 3: Each cow was given the Chinese medicine sustained-release stick prepared in control group 3 once a week for 8 consecutive weeks.

[0063] Control group: fed with a regular daily ration.

[0064] The evaluation criteria are shown in Table 1.

[0065] Table 1 Test Evaluation Criteria

[0066] The results are shown in Table 2. Overall effective rate (%) = (Significantly effective + Effective) / Total number of cases × 100%.

[0067] Table 2. Statistical analysis of the efficacy of traditional Chinese medicine sustained-release sticks

[0068] As can be seen from Tables 1-2, the slow-release herbal stick of the present invention can effectively treat postpartum lochia retention and blood stasis abdominal pain in cattle and horses.

[0069] The parts of this invention not described in detail are prior art. It will be apparent to those skilled in the art that this invention is not limited to the details of the above exemplary embodiments, and that the invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be regarded as exemplary and non-limiting in all respects. The scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all changes falling within the meaning and scope of the equivalents of the claims be included within the invention.

Claims

1. A sustained-release traditional Chinese medicine stick for treating postpartum blood stasis in cattle and horses, characterized by: The product comprises traditional Chinese medicine extracts and a sustained-release regulating matrix. The amount of the traditional Chinese medicine extracts added, by weight, is as follows: 6-12 parts of safflower extract (concentration ratio 10:1), 10-15 parts of peach kernel extract (concentration ratio 10:1), 4-8 parts of cattail pollen extract (concentration ratio 30:1), 6-12 parts of dipsacus extract (concentration ratio 20:1), 4-8 parts of lindera extract (concentration ratio 30:1), 2-5 parts of sparganium extract (concentration ratio 30:1), 2-8 parts of honeysuckle extract (concentration ratio 30:1), 6-12 parts of astragalus extract (concentration ratio 30:1), 1-4 parts of artemisia extract (concentration ratio 30:1), 2-8 parts of corydalis extract (concentration ratio 20:1), and 2-4 parts of white peony extract (concentration ratio 20:1). The sustained-release regulating matrix, by weight, comprises 25-35 parts of a hydrophilic matrix, 85-95 parts of a hydrophobic matrix, and 15-25 parts of a matrix with an amphiphilic structure.

2. The sustained-release traditional Chinese medicine stick for treating postpartum blood stasis in cattle and horses as described in claim 1, characterized in that: The hydrophilic matrix is ​​one of the following: chitosan, hyaluronic acid, polyethylene glycol 4000, sodium alginate, poly(N-isopropylacrylamide), polyoxyethylene, and polyethylene glycol-chitosan copolymer.

3. The sustained-release traditional Chinese medicine stick for treating postpartum blood stasis in cattle and horses as described in claim 1, characterized in that: The hydrophobic matrix is ​​one of the following: hydrophobic matrix acrylate copolymer, calcium stearate, paraffin emulsion, hydrogenated vegetable oil, polyoxyethylene monostearate, silica, beeswax, and octadecyltrimethylammonium bromide.

4. The sustained-release traditional Chinese medicine stick for treating postpartum blood stasis in cattle and horses as described in claim 1, characterized in that: The amphiphilic matrix is ​​one of the following: poloxamer 188, polysorbate 80, polycaprolactone, and polyethylene glycol palmitic acid.

5. The method for preparing the sustained-release traditional Chinese medicine stick for treating postpartum blood stasis in cattle and horses as described in claim 1, characterized in that: Includes the following steps: (1) Weigh each slow-release regulating matrix according to the above proportions, mix them, and obtain mixture A; (2) Weigh each Chinese herbal extract according to the above proportions, mix them, and obtain mixture B; (3) Heat mixture A until it melts and stir until it becomes a liquid; (4) Add mixture B, mix evenly, hot melt injection molding, and condense to obtain a Chinese medicine sustained-release stick for treating postpartum blood stasis in cattle and horses.

6. The method for preparing the sustained-release traditional Chinese medicine stick for treating postpartum blood stasis in cattle and horses as described in claim 5, characterized in that: In steps (3) and (4), the temperature of the heating and melting of the hot-melting column mold is 75-85℃, and the condensation time is 5-10h.

7. The method for preparing the sustained-release traditional Chinese medicine stick for treating postpartum blood stasis in cattle and horses as described in claim 5, characterized in that: Step (4) The size of the Chinese medicine sustained-release stick is adapted to the feeder or rumen retention device.

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