A method for controlling the growth of Cylindrica pseudocystis.

Abstract

This invention discloses a method for controlling the growth of *Dendrobium fusiforme*, comprising: step one, capturing and culturing *Daphnia fusiforme* as individuals; step two, toxicity acclimatization culture of *Daphnia fusiforme*; step three, selection of toxicity acclimatization genotypes of *Daphnia fusiforme*; and step four, controlling the growth of *Dendrobium fusiforme* using toxicity-acclimatized genotypes of *Daphnia fusiforme*. This invention acclimatizes *Daphnia fusiforme* by feeding it with a toxin-producing strain of *Dendrobium fusiforme*, obtaining toxicity-acclimatized genotypes of *Daphnia fusiforme*, thus improving the survival rate and reduction rate of *Daphnia fusiforme* when treating *Dendrobium fusiforme*. Using toxicity-acclimatized genotypes of *Daphnia fusiforme* for biological algae control effectively improves the control effect on the growth of *Dendrobium fusiforme*.

Description

Technical Field

[0001] This invention relates to the field of water treatment technology, and in particular to a method for controlling the growth of Cyclostomeae. Background Technology

[0002] Existing methods for controlling cyanobacteria are mainly developed for Microcystis, but Cynosporium pseudocystis and Microcystis have different characteristics in terms of aggregation on the water surface. Cynosporium pseudocystis usually forms diffuse algal blooms in the water body, that is, it is distributed relatively evenly in the upper layer of the water body. Traditional emergency algae removal technologies such as mechanical dredging and ultrasonic disruption are difficult to achieve good results.

[0003] Biological algae control is an environmentally friendly method of algae suppression, with advantages such as long-term effects, low cost, and ecological friendliness. However, the only thing that needs to be considered is the adaptability of predatory zooplankton (such as Daphnia fusiformis) to the toxicity of Cylindricalium pseudocapsicum. How to enhance the tolerance of Daphnia fusiformis to the toxicity of Cylindricalium pseudocapsicum and strengthen its control effect on the growth of Cylindricalium pseudocapsicum is an urgent problem to be explored and investigated. Therefore, this invention proposes a method for controlling the growth of Cylindricalium pseudocapsicum to solve the problems existing in the prior art. Summary of the Invention

[0004] To address the aforementioned problems, the present invention aims to propose a method for controlling the growth of *Dalbergia fusiforme*. This method involves acclimating *Dalbergia fusiforme* to toxicity through feeding with a toxin-producing strain of *Dalbergia fusiforme*, thereby obtaining a toxicity-acclimated genotype of *Dalbergia fusiforme*. This improves the survival rate and reduction rate of *Dalbergia fusiforme* when used to control *Dalbergia fusiforme*. Utilizing the toxicity-acclimated genotype of *Dalbergia fusiforme* for biological algae control effectively enhances the control effect on *Dalbergia fusiforme* growth.

[0005] To achieve the objectives of this invention, the invention is implemented through the following technical solution: a method for controlling the growth of *Strombophytum truncatum*, comprising the following steps:

[0006] Step 1: Capture *Daphnia fusiforme* from a wild reservoir and culture individual organisms under light conditions;

[0007] Step 2: Randomly select a portion of the toxin-producing strain QDH7 of *Daphnia fusiforme* cultured in BG-11 medium and culture it continuously for 10 days and 30 days to acclimatize the individual strains to toxicity.

[0008] Step 3: Randomly select a portion of the toxicity-domesticated Daphnia fuzzi and select the toxicity-domesticated genotype to obtain the toxicity-domesticated Daphnia fuzzi genotype.

[0009] Step 4: Using the toxic domestication genotype of *Daphnia fuzzata*, biological algae control methods are employed to control the growth of *Strombyx mori*.

[0010] A further improvement is that: in step one, the fuzzy zonal culture is carried out by transferring the fuzzy zonal cells into a 500ml beaker under the conditions of 1200lx light intensity, 25℃ temperature and light cycle L:D=12h:12h.

[0011] A further improvement is that the culture water is sterilized in an autoclave at a temperature of 121°C and a pressure of 110 kPa for 30 minutes and then cooled to 25°C. The culture food is Protozoa protozoa cultured in BG-11 medium at a concentration of 1 mg C / L.

[0012] A further improvement is that: in step three, the selection of toxic acclimatization genotypes specifically involves feeding the toxin-producing strain QD17 of *Strombyx mori* cultured in BG-11 medium, selecting parthenogenetic eggs and continuing to feed them with toxin-producing *Strombyx mori* food, repeating this process for 100 days.

[0013] The beneficial effects of this invention are as follows: This invention acclimates *Daphnia fusiforme* to toxicity by feeding it with a toxin-producing strain of *Cyclocarya pseudocylindrica*, resulting in a toxicity-acclimated genotype of *Daphnia fusiforme*. This improves the survival rate and reduction rate of *Daphnia fusiforme* when used to control *Cyclocarya pseudocylindrica*. The use of the toxicity-acclimated genotype of *Daphnia fusiforme* for biological algae control effectively enhances the control effect on the growth of *Cyclocarya pseudocylindrica*. Attached Figure Description

[0014] Figure 1 This is a flowchart of the method of the present invention. Detailed Implementation

[0015] To enhance understanding of the present invention, the present invention will be further described in detail below with reference to embodiments. These embodiments are only used to explain the present invention and do not constitute a limitation on the scope of protection of the present invention.

[0016] Cylindrosper mopsisraciborskii, an invasive freshwater cyanobacterial algae, has garnered significant attention since its initial discovery in Java, India. This algae can form algal blooms and produce toxins, posing a substantial threat to aquatic life and human health. Initially considered a characteristic species of tropical and subtropical regions, Cylindrosper mopsisraciborskii has, in recent years, been found to have fully invaded temperate regions due to global warming and eutrophication, gradually replacing Microcystis as the most dominant algal bloom species in eutrophic waters, seriously threatening water safety.

[0017] Existing methods for controlling cyanobacteria mainly target Microcystis. However, Cylindrica pseudocystis and Microcystis have different characteristics in terms of surface aggregation. Cylindrica pseudocystis usually forms diffuse algal blooms, which are relatively evenly distributed in the upper and middle layers of the water body. Traditional emergency algae removal technologies such as mechanical harvesting and ultrasonic disruption are difficult to achieve good results. Compared with Microcystis, Cylindrica pseudocystis has an advantage in food quality, which makes the biological control of algae by Cylindrica pseudocystis possible, but there is currently little research on it.

[0018] Biological algae control is an environmentally friendly method of algae suppression, with advantages such as long-term effects, low cost, and ecological friendliness. However, the only thing that needs to be considered is the adaptability of predatory zooplankton (such as Daphnia fusiformis) to the toxicity of Cylindrica pseudocylindrica. How to enhance the tolerance of Daphnia fusiformis to the toxicity of Cylindrica pseudocylindrica and strengthen its control effect on the growth of Cylindrica pseudocylindrica is an issue that urgently needs to be explored and investigated.

[0019] according to Figure 1 As shown, this embodiment provides a method for controlling the growth of *Cyclophorus*, including the following steps:

[0020] Step 1: Capture *Daphnia fusiforme* from a reservoir in the wild and transfer them to 500ml beakers for individual culture under the conditions of 1200lx light intensity, 25℃ temperature and photoperiod L:D = 12h:12h.

[0021] The culture water was sterilized in an autoclave at 121°C and 110 kPa for 30 minutes and then cooled to 25°C. The culture food was Protozoa protozoa cultured in BG-11 medium at a concentration of 1 mg C / L.

[0022] Step 2: Randomly select a portion of the toxin-producing strains of *Daphnia fusiforme* cultured in BG-11 medium, namely QDH7, and conduct continuous culture for 10 days and 30 days to acclimatize the individual strains to their toxicity.

[0023] Step 3: Randomly select a portion of the toxic-acclimated Daphnia fuzzina and feed them with the toxin-producing strain QD17 of Cylindrica pseudocylindrica cultured in BG-11 medium. Select parthenogenetic eggs and continue to feed them with toxin-producing Cylindrica pseudocylindrica food. Repeat this process for 100 days, and select the toxicity-acclimated genotype to obtain Daphnia fuzzina with the toxicity-acclimated genotype.

[0024] Step 4: Using the toxic domestication genotype of *Daphnia fuzzata*, biological algae control methods are employed to control the growth of *Strombyx mori*.

[0025] Example 1

[0026] Individuals of *Daphnia raciborskii* that had undergone 10 days of toxicity acclimatization and those that had not were randomly selected for a single-individual *Cylindrospermopsis raciborskii* elimination experiment. The culture containers were 50 mL beakers, and the experimental water was 40 mL of tap water sterilized in an autoclave. The food treatment consisted of the toxin-producing strain QDH7 of *Cylindrospermopsis raciborskii* at a concentration of 2 mg C / L. One juvenile *Daphnia* less than 24 hours old was placed in each beaker, with 30 replicates per treatment group. After 3 days of treatment, the survival rate of the un-toxicated *Daphnia raciborskii* was 33%, and the population reduction rate of the surviving individuals was 42.00%. In contrast, the survival rate of the *Daphnia raciborskii* in the 10-day toxicity acclimatization group was 75%, and the average population reduction rate of the surviving individuals reached 67.23%, significantly higher than the survival rate and population reduction rate of the un-toxicated group.

[0027] Example 2

[0028] Individuals of *Daphnia fusiforme* that had undergone 30 days of toxicity acclimatization and those that had not were randomly selected for a single-individual *Cylindrospermopsis raciborskii* elimination experiment. Single-individual culture experiments were conducted in 50 mL beakers. The experimental water was 40 mL of tap water sterilized in an autoclave. The food treatment consisted of the non-toxic strain N8 of *Cylindrospermopsis raciborskii* at a concentration of 2 mg C / L. One juvenile *Daphnia fusiforme* less than 24 hours old was placed in each beaker. Each treatment group had 30 replicates. After 3 days of treatment, the survival rate of the un-toxicated *Daphnia fusiforme* was 33%, and the population reduction rate of the surviving individuals was 42.00%. In contrast, the survival rate of the *Daphnia fusiforme* in the 30-day toxicity acclimatization group was 100%, and the average population reduction rate of the surviving individuals reached 78.54%, significantly higher than the survival rate and population reduction rate of the un-toxicated group.

[0029] Example 3

[0030] Individuals of the toxicity-acclimated *Daphnia fusiforme* were randomly selected for a single-individual *Cylindrospermopsis raciborskii* elimination experiment. Single-individual culture experiments were conducted in 50 mL beakers using 40 mL of autoclaved tap water. The food treatment consisted of the toxin-producing *Cylindrospermopsis raciborskii* strain QDH7 at a concentration of 2 mg C / L. One juvenile *Daphnia* less than 24 hours old was placed in each beaker. Each treatment group had 30 replicates. After 3 days of treatment, the survival rate of the unacclimated *Daphnia fusiforme* was 33%, and the population reduction rate of the surviving individuals was 42.00%. In contrast, the survival rate of the *Daphnia fusiforme* in the 30-day toxicity-acclimated treatment group was 100%, and the average population reduction rate of the surviving individuals reached 84.32%, significantly higher than the survival rate and population reduction rate of the unacclimated group, indicating the best results.

[0031] Example 4

[0032] Individuals of the toxicity-acclimated *Daphnia fusiforme* were randomly selected for a single-individual *Cylindrospermopsis raciborskii* elimination experiment. Single-individual culture experiments were conducted in 50 mL beakers using 40 mL of sterilized tap water. The food treatment consisted of the non-toxic *Cylindrospermopsis raciborskii* strain N8 at a concentration of 2 mg C / L. One juvenile *Daphnia* less than 24 hours old was placed in each beaker. Each treatment group had 30 replicates. After 3 days of treatment, the survival rate of the unacclimated group was 75%, with a *Cylindrospermopsis raciborskii* population reduction rate of 53.33%. In contrast, the survival rate of the *Daphnia fusiforme* in the 30-day toxicity-acclimated group was 100%, and the average *Cylindrospermopsis raciborskii* population reduction rate of surviving individuals reached 89.32%, significantly higher than the survival rate and *Cylindrospermopsis raciborskii* population reduction rate of the unacclimated group.

[0033] The toxicity-acclimated Daphnia fuzzi also showed a significant increase in its clearance rate of non-toxic algal strains, indicating that the acclimation process may occur not only in toxicity adaptation but also in food quality adaptation.

[0034] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for controlling growth of Scedosporium apiospermum, characterized by, The method comprises the following steps: Step 1, capturing Daphnia pulex in the wild reservoir, and culturing single individuals under light conditions; Step 2, randomly selecting part of the Daphnia pulex to be cultured by using BG-11 culture medium to produce toxic strain QDH7 for 10 days and 30 days of continuous culture, and individual toxicity domestication is carried out; Step 3, randomly selecting part of the Daphnia pulex after toxicity domestication to select the toxicity domestication genotype, and obtaining the Daphnia pulex with the toxicity domestication genotype; The toxicity domestication genotype selection is specifically feeding by using BG-11 culture medium to culture the Daphnia pulex to produce toxic strain QD17, selecting parthenogenetic eggs to continue feeding with the toxic Daphnia pulex, repeating the process, and continuing for 100 days; Step 4, using the Daphnia pulex with the toxicity domestication genotype to control the growth of Daphnia pulex by using the biological control method.

2. The method of claim 1, wherein the method comprises: The single individual culture of the Daphnia pulex in step 1 is specifically carried out in a 500ml beaker under the conditions of 1200lx light intensity, 25℃ temperature and light cycle L:D=12h:12h.

3. The method of claim 2, wherein the method comprises: The water used in the culture process is obtained by sterilizing the water in a high-pressure sterilization pot at a temperature of 121℃ and a pressure of 110kpa for 30min, and then cooling to 25℃, and the culture food is Chlorella pyrenoidosa cultured by using BG-11 culture medium, and the concentration is 1mg C / L.

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