Microbial agent capable of degrading long-chain fat of kitchen garbage and preparation method of microbial agent

Abstract

The invention discloses a microbial agent capable of degrading long-chain fat in kitchen garbage and a preparation method of the microbial agent, belongs to the technical field of kitchen garbage treatment, and particularly relates to a liquid bacterial solution prepared by mixing a bacillus subtilis solution, a bacillus licheniformis solution and a candida lipolytica solution; the method comprises the following steps: mixing a modified solution containing sodium alginate with peanut shells, then mixing with a conditioner containing coco coir, rice husks and bioactive carbon to prepare a microbial agent, and in the preparation of the bioactive carbon, mixing the modified solution containing sodium alginate, calcium nitrate and the peanut shells, and roasting and carbonizing to prepare the bioactive carbon; in the preparation of the bioactive carbon, an amino compound can also be added, and the amino compound comprises 2-aminothiazole hydrochloride and amino-(2, 4-dihydroxy-phenyl)-acetic acid. The invention provides the microbial agent which is good in microbial survival rate, long in storage time and good in grease degradation effect, and the preparation method of the microbial agent.

Description

Technical Field

[0001] This invention belongs to the field of kitchen waste treatment technology, specifically relating to a microbial agent that can degrade long-chain fats in kitchen waste and its preparation method. Background Technology

[0002] Kitchen waste, also known as organic waste or perishable waste, refers to easily perishable biomass household waste such as food scraps, leftovers, expired food, fruit peels and cores, and flowers and plants. It is characterized by its easy decomposition, low calorific value, and rich organic matter content. Conventional landfill and incineration are difficult to handle properly, easily causing environmental pollution and posing certain safety hazards. Currently, kitchen waste is mainly treated using biodegradation technology. This involves utilizing specialized bio-fermentation equipment to rapidly ferment certain microorganisms with degradation functions at suitable temperatures, transforming kitchen waste into relatively stable humus. By removing carbon dioxide and water vapor, it can ultimately be converted into organic fertilizer. Among the microbial treatments of kitchen waste, aerobic composting technology has gradually become the main method for resource regeneration. The types of microorganisms and their enzyme activity are the main influencing factors on composting maturation time and organic fertilizer quality, primarily degrading proteins, oils, starches, and cellulose. However, existing technologies generally suffer from poor degradation effects. Therefore, developing microorganisms that can efficiently degrade the main components of kitchen waste and determining their optimal degradation conditions can better serve multiple aspects such as agriculture and urban pollution control, and maximize resource utilization. Summary of the Invention

[0003] The purpose of this invention is to provide a microbial agent for degrading kitchen waste grease with good microbial survival rate, long storage time and good grease degradation effect, and its preparation method.

[0004] The technical solution adopted by the present invention to achieve the above objectives is as follows: A microbial agent includes: a liquid bacterial solution and a conditioner, wherein the amount of the liquid bacterial solution used is 50-100 wt% of the conditioner; the conditioner includes bio-activated carbon containing alginate and calcium carbides. Mixing the liquid bacterial solution and the conditioner, wherein the conditioner is a porous substance with high specific surface area and adsorption capacity, and the liquid bacterial solution is dispersed in the conditioner, can improve the structure of the resulting microbial agent and enhance its degradation of organic matter in kitchen waste.

[0005] Preferably, the bioactive carbon also contains carbides of amino compounds; or, the bioactive carbon also contains carbides of peanut shells.

[0006] Preferably, the conditioner also includes coconut coir and / or rice husks.

[0007] More preferably, the amount of rice husk used is 40-60 wt% of coconut coir; or the amount of bio-activated carbon used is 80-120 wt% of coconut coir.

[0008] Preferably, the liquid bacterial solution contains at least one of Bacillus cereus, Bacillus licheniformis, and Candida lipolyticis.

[0009] This invention discloses a method for preparing a microbial inoculant, comprising: mixing a liquid bacterial solution with a conditioner to prepare a microbial inoculant; the amount of liquid bacterial solution used is 50-100 wt% of the conditioner; the conditioner includes bio-activated carbon, which is prepared by carbonizing a modified solution, calcium nitrate, and peanut shells; the modified solution contains sodium alginate, and the content of sodium alginate in the modified solution is 5-20 wt%.

[0010] Preferably, the solvent of the modified solution is an aqueous ethanol solution, and the content of ethanol in the aqueous ethanol solution is 30-70 wt%.

[0011] Preferably, the liquid bacterial solution is a mixture of Bacillus subtilis solution, Bacillus licheniformis solution and Candida lipolyticis solution, wherein the Bacillus subtilis solution, Bacillus licheniformis solution and Candida lipolyticis solution are mixed in a volume ratio of 1:0.1-10:0.1-10.

[0012] Preferably, the modified solution further contains an amino compound, including 2-aminothiazole hydrochloride and / or amino-(2,4-dihydroxyphenyl)-acetic acid. This invention prepares bioactive carbon from peanut shells, sodium alginate, calcium nitrate, and 2-aminothiazole hydrochloride and / or amino-(2,4-dihydroxyphenyl)-acetic acid. The calcium in sodium alginate and calcium nitrate forms a cross-linked structure. Then, with the combined use of 2-aminothiazole hydrochloride and / or amino-(2,4-dihydroxyphenyl)-acetic acid, a bioactive carbon containing a carbide of calcium alginate, 2-aminothiazole hydrochloride, and / or amino-(2,4-dihydroxyphenyl)-acetic acid is formed. When mixed with coconut coir and / or rice husks, this improves the storage performance of the prepared microbial agent, increases the survival rate of the microorganisms after storage, and, when applied to kitchen waste treatment, enhances the degradation rate of grease in kitchen waste.

[0013] More preferably, the amount of 2-aminothiazole hydrochloride used is 10-30 wt% of sodium alginate, and the amount of amino-(2,4-dihydroxy-phenyl)-acetic acid used is 4-24 wt% of sodium alginate.

[0014] Preferably, the preparation of the microbial agent includes the preparation of a modified solution.

[0015] More preferably, in the preparation of the modified solution, alginate is added to an aqueous ethanol solution and mixed to obtain the modified solution.

[0016] More preferably, in the preparation of the modified solution, the ethanol content in the aqueous ethanol solution is 30-70 wt%.

[0017] More preferably, in the preparation of the modified solution, the alginate is sodium alginate, and the content of sodium alginate in the modified solution is 5-20 wt%.

[0018] More preferably, an amino compound is added during the preparation of the modified solution. The amino compound is 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid.

[0019] More preferably, in the preparation of the modified solution, the amount of 2-aminothiazole hydrochloride used is 10-30 wt% of sodium alginate, and the amount of amino-(2,4-dihydroxy-phenyl)-acetic acid used is 4-24 wt% of sodium alginate.

[0020] Preferably, the preparation of the microbial agent includes the preparation of bioactive carbon.

[0021] More preferably, in the preparation of bioactive carbon, peanut shells are cleaned, crushed and sieved to obtain peanut shell powder, then a modification solution and calcium nitrate are added, and the mixture is allowed to stand at 20-40℃ for 6-24 hours, and then dried and calcined to prepare bioactive carbon.

[0022] More preferably, in the preparation of bioactive carbon, the sieving is performed through a 40-60 mesh sieve.

[0023] More preferably, in the preparation of bio-activated carbon, the amount of modified solution used is measured by the amount of alginate used, which is 10-40 wt% of peanut shell powder.

[0024] More preferably, in the preparation of bio-activated carbon, the amount of calcium nitrate used is 5-20 wt% of peanut shell powder.

[0025] More preferably, calcium cyclohexanesulfonate can be added to the modified solution in the bioactive carbon, with the amount of calcium cyclohexanesulfonate being 1-10 wt% of sodium alginate. After using 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid in the preparation of bioactive carbon, calcium cyclohexanesulfonate can be further added. After further carbonization under the mixed action of calcium alginate, bioactive carbon is obtained. When mixed with coconut coir and / or rice husk, the storage performance of the prepared microbial agent and the survival rate of microorganisms after storage can be further improved. Furthermore, when applied to the treatment of kitchen waste, it can also improve the degradation rate of grease in kitchen waste.

[0026] Preferably, the preparation of the microbial agent includes a liquid culture medium.

[0027] More preferably, the liquid culture medium contains 5-20 g / L peptone, 2-10 g / L yeast extract, 5-20 g / L sodium chloride, 0.05-0.5 g / L magnesium chloride, and 0.005-0.05 g / L ferrous sulfate heptahydrate, with water as the solvent and the pH adjusted to neutral.

[0028] Preferably, the preparation of the microbial agent includes the preparation of the strain propagation solution.

[0029] More preferably, in the preparation of the strain expansion broth, the kitchen waste degrading bacteria are inoculated into a liquid culture medium and cultured at 20-40℃ for 12-24h to obtain a seed culture. Then, the same inoculation amount is inoculated into a new liquid culture medium for expansion culture and cultured at 20-40℃ for 12-24h to obtain the strain expansion broth.

[0030] More preferably, in the preparation of the bacterial culture medium, the inoculum amount of kitchen waste degrading bacteria is 1-10 wt% of the liquid culture medium.

[0031] More preferably, in the preparation of the bacterial culture medium, the food waste degrading bacteria include Bacillus subtilis, Bacillus licheniformis, and Candida lipolyticis.

[0032] Preferably, the preparation of the microbial agent includes the preparation of a liquid bacterial solution.

[0033] More preferably, in the preparation of liquid bacterial solution, the bacterial strain culture medium is mixed to prepare liquid bacterial solution.

[0034] More preferably, in the preparation of the liquid bacterial solution, the bacterial culture medium includes Bacillus subtilis culture medium, Bacillus licheniformis culture medium and Candida lipolyticis culture medium, and the Bacillus subtilis culture medium, Bacillus licheniformis culture medium and Candida lipolyticis culture medium are mixed in a volume ratio of 1:0.1-10:0.1-10.

[0035] Preferably, in the preparation of the microbial agent, the liquid bacterial solution is mixed with a conditioner to prepare the microbial agent.

[0036] More preferably, in the preparation of the microbial inoculant, the conditioning agent includes coconut coir, rice husk and bio-activated carbon.

[0037] More preferably, in the preparation of the microbial agent, the amount of rice husk used in the conditioner is 40-60 wt% of coconut coir.

[0038] More preferably, in the preparation of the microbial agent, the amount of bioactive carbon used in the conditioner is 80-120 wt% of coconut coir.

[0039] More preferably, in the preparation of microbial agents, the amount of liquid bacterial solution used is 50-100 wt% of the conditioner.

[0040] This invention discloses the use of the above-mentioned microbial agent in the degradation of kitchen waste.

[0041] This invention utilizes a method of preparing a liquid bacterial solution by mixing Bacillus subtilis, Bacillus licheniformis, and Candida lipolytica, and then mixing this solution with a conditioner containing coconut coir, rice husks, and bioactive carbon to prepare a microbial agent. In the preparation of the bioactive carbon, a modified solution containing sodium alginate, calcium nitrate, and peanut shells are mixed and then calcined and carbonized to obtain the bioactive carbon. Furthermore, amino compounds, including 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid, can be added during the preparation of the bioactive carbon. Therefore, this invention provides the following beneficial effects: high microbial survival rate, good storage performance of the prepared microbial agent, and good grease degradation rate when applied to kitchen waste treatment. Thus, this invention is a microbial agent and its preparation method for degrading kitchen waste grease, characterized by high microbial survival rate, long storage time, and good grease degradation effect. Attached Figure Description

[0042] Figure 1 This is an electron micrograph of bioactive carbon.

[0043] Figure 2 This is a graph showing the effective viable bacterial survival rate.

[0044] Figure 3 This is a graph showing the rate of oil degradation. Detailed Implementation

[0045] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0046] The concepts involved in this application will first be described with reference to the accompanying drawings. It should be noted that the following descriptions of various concepts are only for the purpose of making the content of this application easier to understand and do not constitute a limitation on the scope of protection of this application; furthermore, the embodiments and features in the embodiments of this application can be combined with each other unless otherwise specified. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0047] Example 1: A method for preparing a microbial inoculant Preparation of the modified solution: Alginate was added to an aqueous ethanol solution to obtain the modified solution. The ethanol content in the aqueous ethanol solution was 40 wt%, and the alginate was sodium alginate, with the sodium alginate content in the modified solution being 10 wt%.

[0048] Preparation of bioactive carbon: Peanut shells were cleaned, crushed, and sieved to obtain peanut shell powder. A modification solution and calcium nitrate were then added, and the mixture was allowed to stand at 30°C for 12 hours. After drying and calcination, bioactive carbon was obtained. The sieve was a 50-mesh sieve. The amount of the modification solution used was measured by the amount of alginate, which was 30 wt% of the peanut shell powder, and the amount of calcium nitrate was 10 wt% of the peanut shell powder.

[0049] Liquid culture medium: 10 g / L peptone, 5 g / L yeast extract, 10 g / L sodium chloride, 0.2 g / L magnesium chloride, 0.01 g / L ferrous sulfate heptahydrate, with water as the solvent and pH adjusted to neutral.

[0050] Preparation of the bacterial culture medium: The food waste-degrading bacteria were inoculated into liquid culture medium and cultured at 37℃ for 16 hours to obtain a seed culture. Then, the same inoculation amount was inoculated into fresh, identical liquid culture medium for expansion culture, and cultured at 37℃ for 16 hours to obtain the bacterial culture medium. The inoculation amount of the food waste-degrading bacteria was 10 wt% of the liquid culture medium. The food waste-degrading bacteria included *Bacillus subtilis*, *Bacillus licheniformis*, and *Candida lipolyticis*. *Bacillus subtilis* has the accession number GDMCC1.5711. *Bacillus licheniformis* has the accession number GDMCC1.5713. *Candida lipolyticis* has the accession number GDMCC2.3.

[0051] Preparation of liquid bacterial culture: The bacterial culture solutions were mixed to prepare the liquid bacterial culture. The bacterial culture solutions included Bacillus subtilis culture solution, Bacillus licheniformis culture solution, and Candida lipolyticis culture solution, which were mixed in a volume ratio of 1:1:1.

[0052] Preparation of microbial inoculant: The liquid bacterial solution is mixed with a conditioner to prepare the microbial inoculant. The conditioner includes coconut coir, rice husk, and bio-activated carbon. The amount of rice husk used in the conditioner is 50 wt% of the amount of coconut coir, the amount of bio-activated carbon used in the conditioner is 100 wt% of the amount of coconut coir, and the amount of liquid bacterial solution used is 100 wt% of the amount of conditioner.

[0053] Example 2: A method for preparing a microbial inoculant The difference between this embodiment and Example 1 lies in the preparation of the modified solution.

[0054] Preparation of the modified solution: Alginate and amino compounds were mixed in an ethanol-water solution to obtain the modified solution. The ethanol content in the ethanol-water solution was 40 wt%, the alginate was sodium alginate, and the sodium alginate content in the modified solution was 10 wt%; the amino compounds were 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid, with 20 wt% of sodium alginate being 2-aminothiazole hydrochloride and 12 wt% of sodium alginate being amino-(2,4-dihydroxyphenyl)-acetic acid.

[0055] Example 3: A method for preparing a microbial inoculant The difference between this embodiment and Example 2 lies in the preparation of the modified solution.

[0056] Preparation of the modified solution: The modified solution was obtained by mixing alginate and amino compound in an aqueous ethanol solution. The ethanol content in the aqueous ethanol solution was 40 wt%, the alginate was sodium alginate, and the sodium alginate content in the modified solution was 10 wt%; the amino compound was 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid, with the amount of 2-aminothiazole hydrochloride being 15 wt% of sodium alginate and the amount of amino-(2,4-dihydroxyphenyl)-acetic acid being 10 wt% of sodium alginate.

[0057] Example 4: A method for preparing a microbial inoculant The difference between this embodiment and Example 2 lies in the preparation of the modified solution.

[0058] Preparation of the modified solution: Alginate and amino compounds were mixed in an ethanol-water solution to obtain the modified solution. The ethanol-water solution contained 40 wt% ethanol, and the alginate was sodium alginate, which contained 10 wt% sodium alginate in the modified solution. The amino compounds were 2-aminothiazole hydrochloride, amino-(2,4-dihydroxy-phenyl)-acetic acid, and calcium cyclohexanesulfonate. The amount of 2-aminothiazole hydrochloride used was 20 wt% of sodium alginate, the amount of amino-(2,4-dihydroxy-phenyl)-acetic acid used was 12 wt% of sodium alginate, and the amount of calcium cyclohexanesulfonate used was 7 wt% of sodium alginate.

[0059] Example 5: A method for preparing a microbial inoculant The difference between this embodiment and Example 2 lies in the preparation of the modified solution.

[0060] Preparation of the modified solution: Alginate and amino compounds were mixed in an ethanol-water solution to obtain the modified solution. The ethanol-water solution contained 40 wt% ethanol, sodium alginate was used, and the sodium alginate content in the modified solution was 10 wt%. The amino compounds were 2-aminothiazole hydrochloride, amino-(2,4-dihydroxy-phenyl)-acetic acid, and calcium cyclohexanesulfonate. The amount of 2-aminothiazole hydrochloride used was 20 wt% of sodium alginate, the amount of amino-(2,4-dihydroxy-phenyl)-acetic acid used was 12 wt% of sodium alginate, and the amount of calcium cyclohexanesulfonate used was 3 wt% of sodium alginate.

[0061] Comparative Example 1: A method for preparing a microbial inoculant The difference between this comparative example and Example 1 lies in the preparation of the modified solution.

[0062] Preparation of the modified solution: Alginate and amino compound were mixed in an ethanol-water solution to obtain the modified solution. The ethanol content in the ethanol-water solution was 40 wt%, the alginate was sodium alginate, and the sodium alginate content in the modified solution was 10 wt%; the amino compound was 2-aminothiazole hydrochloride, and the amount of 2-aminothiazole hydrochloride used was 20 wt% of sodium alginate.

[0063] Comparative Example 2: A method for preparing a microbial inoculant The difference between this comparative example and Example 1 lies in the preparation of the modified solution.

[0064] Preparation of the modified solution: The modified solution was obtained by mixing alginate and amino compound in an aqueous ethanol solution. The ethanol content in the aqueous ethanol solution was 40 wt%, the alginate was sodium alginate, and the sodium alginate content in the modified solution was 10 wt%; the amino compound was amino-(2,4-dihydroxy-phenyl)-acetic acid, and the amount of amino-(2,4-dihydroxy-phenyl)-acetic acid used was 12 wt% of the sodium alginate.

[0065] Comparative Example 3: A method for preparing a microbial inoculant The difference between this comparative example and Example 1 lies in the preparation of the modified solution.

[0066] Preparation of the modified solution: The modified solution was obtained by mixing alginate and amino compound in an aqueous ethanol solution. The ethanol content in the aqueous ethanol solution was 40 wt%, the alginate was sodium alginate, and the sodium alginate content in the modified solution was 10 wt%; the amino compound was 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, with 2-aminothiazole hydrochloride used at 5 wt% of sodium alginate and amino-(2,4-dihydroxy-phenyl)-acetic acid used at 2 wt% of sodium alginate.

[0067] Experimental example: 1. Electron microscopy characterization The morphology of the bioactive carbon prepared in Example 1 was characterized by SEM, and the results are as follows: Figure 1 As shown, the surface of bio-activated carbon is rough and uneven, and there are large bumps and pores on the surface.

[0068] 2. Storage performance test The microbial agents prepared in the examples and comparative examples were packaged in bags, vacuum-sealed, and stored at 4°C for 180 days. The number of viable bacteria in the agents was then determined. The total viable bacteria count was determined using the dilution-coating method, with the number of viable bacteria on day 0 defined as 100%. The viable bacteria survival rate after 180 days was tested, and the results are as follows: Figure 2As shown, S1 is Example 1, S2 is Example 2, S3 is Example 3, S4 is Example 4, S5 is Example 5, D1 is Comparative Example 1, D2 is Comparative Example 2, and D3 is Comparative Example 3. This invention prepares a liquid bacterial solution by mixing Bacillus subtilis liquid, Bacillus licheniformis liquid, and Candida lipolytica liquid. This solution is then mixed with a conditioner containing coconut coir, rice husk, and bioactive carbon to prepare a microbial agent. In the preparation of the bioactive carbon, a modified solution containing sodium alginate, calcium nitrate, and peanut shells are mixed and then calcined and carbonized to obtain bioactive carbon. In the preparation of bioactive carbon, amino compounds, including 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid, can be added. These amino compounds are then mixed with sodium alginate, calcium nitrate, and peanut shells and calcined to produce bioactive carbon. After adding 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid to prepare bioactive carbon, this bioactive carbon is then applied to the preparation of microbial agents. The resulting microbial agents exhibit a higher survival rate of viable bacteria after long-term storage. However, using only 2-aminothiazole hydrochloride or amino-( When bioactive carbon is obtained by using either 2,4-dihydroxy-phenyl)-acetic acid, the survival rate of viable microorganisms in the resulting microbial agent does not significantly improve after long-term storage. Furthermore, even when bioactive carbon is prepared using both 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, if the amounts of 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid used are too low, the survival rate of viable microorganisms in the resulting microbial agent also does not significantly improve after long-term storage. Under certain usage conditions, increasing the amounts of 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid can improve the survival rate of effective live bacteria in the obtained microbial agent after long-term storage. In addition to using 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, calcium cyclohexanesulfonate can also be added. After preparing bioactive carbon from 2-aminothiazole hydrochloride, amino-(2,4-dihydroxy-phenyl)-acetic acid, and calcium cyclohexanesulfonate, the survival rate of effective live bacteria in the prepared microbial agent is further improved after long-term storage.

[0069] 3. Oil degradation rate The simulated kitchen waste included: cabbage, rice, apple peels, fatty pork, soybean oil, sodium chloride, and water. The cabbage accounted for 40 wt% of the total weight, the rice for 20 wt%, the apple peels for 10 wt%, the fatty pork for 10 wt%, the soybean oil for 3 wt%, and the sodium chloride for 0.05 wt%, with the remainder being water. All the cabbage, rice, apple peels, and fatty pork were chopped and processed.

[0070] The determination of grease in simulated kitchen waste was performed using acid hydrolysis and ether extraction. First, the simulated kitchen waste was air-dried under a uniform mixing state. 2g of the air-dried sample was taken, and then 8mL of water and 10mL of concentrated hydrochloric acid were added and mixed thoroughly. The mixture was then stirred and digested at 70℃ for 1 hour. Afterward, 10mL of ethanol was added and mixed thoroughly, followed by 25mL of ether. The mixture was shaken thoroughly and allowed to stand until the solution naturally separated into layers. The supernatant was collected, dried to constant weight, and the grease weight was recorded as the initial grease content. The microbial agents prepared in the examples and comparative examples were added to the simulated kitchen waste. The amount of microbial agent was 250g. The amount of simulated kitchen waste added on the first day was 500g, and 500g of simulated kitchen waste was added daily for 7 consecutive days. On the 8th day, the grease content of the treated kitchen waste was measured, and the grease degradation rate was calculated. The results are as follows: Figure 3As shown, S1 is Example 1, S2 is Example 2, S3 is Example 3, S4 is Example 4, S5 is Example 5, D1 is Comparative Example 1, D2 is Comparative Example 2, and D3 is Comparative Example 3. This invention prepares a liquid bacterial solution by mixing Bacillus subtilis liquid, Bacillus licheniformis liquid, and Candida lipolytica liquid. This solution is then mixed with a conditioner containing coconut coir, rice husk, and bioactive carbon to prepare a microbial agent. In the preparation of the bioactive carbon, a modified solution containing sodium alginate, calcium nitrate, and peanut shells are mixed and then calcined and carbonized. Bioactive carbon can be prepared by adding amino compounds, including 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid, and then mixing the amino compounds with sodium alginate, calcium nitrate, and peanut shells and calcining them to produce bioactive carbon. After adding 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid to prepare bioactive carbon, its application in the preparation of microbial agents results in microbial agents with higher oil degradation rates. However, using only 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid... When either 2-aminothiazole hydrochloride or amino-(2,4-dihydroxyphenyl)-acetic acid is used to prepare bioactive carbon, the lipid degradation rate of the resulting microbial agent is not significantly improved. Furthermore, even when both 2-aminothiazole hydrochloride and amino-(2,4-dihydroxyphenyl)-acetic acid are used simultaneously to prepare bioactive carbon, if the amounts of either are too low, the lipid degradation rate of the resulting microbial agent is also not significantly improved. Under certain usage conditions, increasing the amounts of 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid can improve the lipid degradation rate of the obtained microbial agent. In addition to using 2-aminothiazole hydrochloride and amino-(2,4-dihydroxy-phenyl)-acetic acid, calcium cyclohexanesulfonate can also be added. After preparing bioactive carbon from 2-aminothiazole hydrochloride, amino-(2,4-dihydroxy-phenyl)-acetic acid, and calcium cyclohexanesulfonate, the lipid degradation rate of the prepared microbial agent is further improved.

[0071] The embodiments and / or implementation methods described above are merely preferred embodiments and / or implementation methods for implementing the technology of the present invention, and are not intended to limit the implementation methods of the technology of the present invention in any way. Any person skilled in the art can make some modifications or alterations to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as the technology or embodiments that are substantially the same as the present invention.

[0072] This document uses specific examples to illustrate the principles and implementation methods of this application. The descriptions of the above embodiments are only for the purpose of helping to understand the methods and core ideas of this application. The above descriptions are only preferred embodiments of this application. It should be noted that due to the limitations of written expression, while there are objectively infinite specific structures, those skilled in the art can make several improvements, modifications, or changes without departing from the principles of this application, and can also combine the above technical features in an appropriate manner. These improvements, modifications, changes, or combinations, or the direct application of the inventive concept and technical solution to other situations without modification, should all be considered within the scope of protection of this application.

Claims

1. A microbial inoculant, comprising: Liquid bacterial solution and conditioner, with the amount of liquid bacterial solution used being 50-100 wt% of the conditioner; the conditioner includes bio-activated carbon, which contains alginate and calcium carbides.

2. The microbial inoculant according to claim 1, characterized in that, The bioactive carbon also contains carbides of amino compounds; or, the bioactive carbon also contains carbides of peanut shells.

3. The microbial inoculant according to claim 1, characterized in that, The conditioning agent also includes coconut coir and / or rice husks.

4. The microbial inoculant according to claim 3, characterized in that, The amount of rice husk used is 40-60 wt% of coconut coir; or the amount of bio-activated carbon used is 80-120 wt% of coconut coir.

5. The microbial inoculant according to claim 1, characterized in that, The liquid bacterial solution contains at least one of Bacillus cereus, Bacillus licheniformis, and Candida lipolyticis.

6. A method for preparing a microbial inoculant, comprising: The microbial inoculant is prepared by mixing liquid bacterial solution with a conditioner. The amount of liquid bacterial solution used is 50-100 wt% of the conditioner; the conditioner includes bio-activated carbon, which is prepared by mixing modified solution, calcium nitrate and peanut shells and then carbonizing them. The modified solution contains sodium alginate, and the content of sodium alginate in the modified solution is 5-20 wt%.

7. The method for preparing a microbial inoculant according to claim 6, characterized in that, The solvent of the modified solution is an aqueous ethanol solution, and the content of ethanol in the aqueous ethanol solution is 30-70 wt%.

8. The method for preparing a microbial inoculant according to claim 6, characterized in that, The liquid bacterial solution is composed of Bacillus subtilis solution, Bacillus licheniformis solution and Candida lipolyticis solution, which are mixed in a volume ratio of 1:0.1-10:0.1-10.

9. The method for preparing a microbial inoculant according to claim 6, characterized in that, The modified solution also contains amino compounds, including 2-aminothiazole hydrochloride and / or amino-(2,4-dihydroxyphenyl)-acetic acid.

10. The use of the microbial agent according to claim 1 in the degradation of kitchen waste.

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