Pet bone joint protective agent based on chondroitin LPM penetration

Abstract

The invention relates to the technical field of pet joint protection, and discloses a chondroitin LPM penetration-based pet bone joint protective agent which comprises the following components in percentage by weight: 1-30% of a carrier, 3-4% of water, 1-30% of a food calling component and 1-30% of a functional component, compared with the prior art, the edible absorption rate, the permeability and the activity rate of the protective agent disclosed by the invention are greatly improved compared with those of a traditional product, so that the edible effect is improved, the anti-inflammatory efficiency is improved, and the pet bone articular cartilage can be conveniently repaired and pains can be conveniently relieved.

Description

Technical Field

[0001] This invention relates to the field of pet joint protection technology, specifically to a pet bone and joint protectant based on chondroitin LPM penetration. Background Technology

[0002] With the increasing popularity of pet ownership and the growing attention people pay to pet health, pet skeletal health issues are receiving more and more attention. Pet arthritis is a chronic, systemic, inflammatory disease in pets such as dogs and cats, mainly involving the movable joints of the limbs. Clinical manifestations include swelling or lameness of the wrist, knee, and tarsal joints, edema, heat, and fluctuation of the soft tissues around the joints. The incidence rate increases with age, and the symptoms become more obvious.

[0003] Currently, there are various chondroitin supplements on the market for pet bone health. Most of them are mainly composed of minerals such as calcium and phosphorus, supplemented with absorption-promoting factors such as vitamin D. However, while these traditional solutions can enhance bone strength to a certain extent, they often neglect the importance of protecting joint cartilage, reducing inflammation, and promoting cartilage repair. Traditional chondroitin products use high-molecular-weight chondroitin sulfate, which is difficult to penetrate the cell membrane barrier. This results in low absorption and anti-inflammatory efficiency. In the acidic environment of the stomach, the collagen peptide molecular structure of traditional protectants is unstable, leading to a high loss rate. Furthermore, the synergistic effect of active ingredients lacks an effective carrier. Therefore, we provide a pet bone and joint protectant based on chondroitin LPM penetration to solve this problem. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a pet bone and joint protectant based on chondroitin LPM penetration, thereby solving this problem.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a pet bone and joint protectant based on chondroitin LPM penetration, comprising the following components: carrier, water, palatability-enhancing ingredient and functional ingredient, wherein the components of the material by weight percentage are as follows: carrier 1%-30%, water 3-4%, palatability-enhancing ingredient 1%-30%, and functional ingredient 1-30%.

[0006] As a further aspect of the present invention: the carrier is composed of dextrin and corn starch.

[0007] As a further aspect of the present invention: the palatability-enhancing ingredient is composed of meat products, meat product hydrolysate, and yeast extract.

[0008] As a further aspect of the present invention: the functional components are composed of a mixture of chondroitin sulfate, green-lipped mussel, krill, oyster, collagen, turmeric, frankincense, black pepper extract and hyaluronic acid.

[0009] As a further aspect of the present invention: the chondroitin sulfate is a small molecule chondroitin sulfate, which is produced by bio-enzymatic hydrolysis of large molecule chondroitin sulfate. The molecular weight of the small molecule chondroitin sulfate is <1000 Da, and the raw material for the small molecule chondroitin sulfate is selected from any one or more of shark, cow and chicken.

[0010] As a further aspect of the present invention, the paste is selected from supramolecular complex cyclodextrin inclusion complexes.

[0011] This invention also provides a method for preparing a pet osteoarthritis protectant based on chondroitin LPM penetration, comprising the following steps:

[0012] S1. Preparation: Prepare sufficient raw materials for carriers, palatability-enhancing ingredients, and some functional ingredients;

[0013] S2. Raw material preparation: High-purity collagen peptide powder (large molecular weight chondroitin sulfate) is extracted from any one or more of shark, cow and chicken, and then broken down into small molecular weight chondroitin sulfate through modern enzymatic hydrolysis, with a molecular weight <1000Da.

[0014] S3. Premix: The powdered yeast extract from the food-attracting substance and the small molecule chondroitin sulfate, collagen, turmeric, and black pepper extract from the functional ingredients are stirred and premixed to obtain the premix.

[0015] S4. Mixing: Add all the pretreated carriers, water, meat products and their hydrolysates from the attractant, and green-lipped mussels, krill, oysters, frankincense, and hyaluronic acid from the functional ingredients to the premix and stir until well mixed. The stirring speed is 300-500 rpm and the stirring time is 3-5 minutes.

[0016] S5. Drying and Discharging: After drying the semi-finished product obtained after mixing, cut it into equal portions and discharge it. The drying temperature is 75-90℃ and the time is 20-30 minutes.

[0017] Compared with the prior art, the present invention provides a pet bone and joint protectant based on chondroitin LPM penetration, which has the following beneficial effects:

[0018] The protective agent of this invention utilizes enzymatic hydrolysis technology to break down macromolecular chondroitin sulfate (molecular weight > 3000 Da) into small molecule (LPM) chondroitin sulfate, of which > 90% have a molecular weight < 1000 Da. The efficacy of small molecule chondroitin sulfate far surpasses that of macromolecular chondroitin sulfate. Furthermore, the designed supramolecular complex cyclodextrin inclusion complex effectively alters the physicochemical properties of the active ingredient and increases the effective carrier for the synergistic effect of the active ingredient. The molecular weight combination of the designed small molecule (LPM) chondroitin sulfate and the supramolecular complex cyclodextrin inclusion complex enhances its efficiency in penetrating biological barriers, ensuring the stability of the composite structure within the pH range of 2-8 and maintaining gastric acid tolerance. This effectively improves the product's absorption rate, penetration rate, and activity rate, enhancing its efficacy, anti-inflammatory efficiency, and facilitating the repair of pet bone and joint cartilage and pain relief. Detailed Implementation

[0019] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0020] Example 1

[0021] A pet bone and joint protectant based on chondroitin LPM penetration comprises the following components: carrier, water, palatability-enhancing ingredient, and functional ingredient, wherein the components of the material by weight percentage are as follows: carrier 30%, water 4%, palatability-enhancing ingredient 30%, and functional ingredient 30%.

[0022] S1. Preparation: Prepare sufficient raw materials for carriers, palatability-enhancing ingredients, and some functional ingredients;

[0023] S2. Raw material preparation: High-purity collagen peptide powder (large molecular weight chondroitin sulfate) extracted from sharks is broken down into small molecular weight chondroitin sulfate through modern enzymatic hydrolysis, with a molecular weight <1000 Da.

[0024] S3. Premix: The powdered materials of 5% yeast extract in the food-attracting substance and 13% chondroitin sulfate, 2% collagen, 3% turmeric, and 1% black pepper extract in the functional ingredients are stirred and premixed to obtain the premix.

[0025] S4. Mixing: Add the carrier composed of 15% corn starch and 15% dextrin selected from supramolecular complex cyclodextrin inclusion complex, 4% water, 15% meat products and 10% hydrolysate from the attractant, and 2% green-lipped mussel, 3% krill, 2% oyster, 2% frankincense, and 2% hyaluronic acid from the functional ingredients to the premix and stir until homogeneous at 500 rpm for 5 min.

[0026] S5. Drying and Discharging: After drying the semi-finished product obtained after mixing, cut it into equal portions and discharge it. The drying temperature is 90℃ and the time is 30min.

[0027] Example 2

[0028] A pet bone and joint protectant based on chondroitin LPM penetration comprises the following components: carrier, water, palatability-enhancing ingredient, and functional ingredient, wherein the components of the material by weight percentage are as follows: carrier 5%, water 4%, palatability-enhancing ingredient 25%, and functional ingredient 25%.

[0029] S1. Preparation: Prepare sufficient raw materials for carriers, palatability-enhancing ingredients, and some functional ingredients;

[0030] S2. Raw material preparation: High-purity collagen peptide powder (large molecular weight chondroitin sulfate) extracted from sharks is broken down into small molecular weight chondroitin sulfate through modern enzymatic hydrolysis, with a molecular weight <1000 Da.

[0031] S3, Premix: The powdered materials of 3% yeast extract in the food-attracting substance and 10% chondroitin sulfate, 1% collagen, 3% turmeric, and 2% black pepper extract in the functional ingredients are stirred and premixed to obtain the premix.

[0032] S4. Mixing: Add the carrier (composed of 2% corn starch and 3% dextrin selected from supramolecular complex cyclodextrin inclusion complexes), 4% water, 15% meat products and 7% hydrolysate from the attractant, and 2% green-lipped mussel, 1.5% krill, 2% oyster, 2% frankincense, and 1.5% hyaluronic acid from the functional ingredients to the premix and stir until homogeneous at 400 rpm for 4 minutes.

[0033] S5. Drying and Discharging: The semi-finished product obtained after mixing is dried and then cut into equal portions for discharge. The drying temperature is 85℃ and the time is 27 minutes.

[0034] Example 3

[0035] A pet bone and joint protectant based on chondroitin LPM penetration comprises the following components: carrier, water, palatability-enhancing ingredient, and functional ingredient, wherein the components of the material by weight percentage are as follows: carrier 1%, water 3%, palatability-enhancing ingredient 1%, and functional ingredient 5%.

[0036] S1. Preparation: Prepare sufficient raw materials for carriers, palatability-enhancing ingredients, and some functional ingredients;

[0037] S2. Raw material preparation: High-purity collagen peptide powder (large molecular weight chondroitin sulfate) extracted from sharks is broken down into small molecular weight chondroitin sulfate through modern enzymatic hydrolysis, with a molecular weight <1000 Da.

[0038] S3, Premix: The powdered ingredients of 0.3% yeast extract from the food-attracting substance and 2.5% chondroitin sulfate, 0.3% collagen, 0.4% turmeric, and 0.2% black pepper extract from the functional ingredients are stirred and premixed to obtain the premix.

[0039] S4. Mixing: Add the carrier consisting of 0.5% corn starch and 0.5% dextrin selected from supramolecular complex cyclodextrin inclusion complex, 3% water, and the attractant ingredients of 0.4% meat products and 0.3% hydrolysate, and the functional ingredients of 0.4% green-lipped mussel, 0.5% krill, 0.2% oyster, 0.2% frankincense, and 0.3% hyaluronic acid to the premix and stir until homogeneous at 350 rpm for 4 minutes.

[0040] S5. Drying and Discharging: After drying the semi-finished product obtained after mixing, cut it into equal portions and discharge it. The drying temperature is 80℃ and the time is 25min.

[0041] Example 4

[0042] A pet bone and joint protectant based on chondroitin LPM penetration comprises the following components: carrier, water, palatability-enhancing ingredient, and functional ingredient, wherein the components of the material by weight percentage are as follows: carrier 15%, water 3%, palatability-enhancing ingredient 30%, and functional ingredient 20%.

[0043] S1. Preparation: Prepare sufficient raw materials for carriers, palatability-enhancing ingredients, and some functional ingredients;

[0044] S2. Raw material preparation: High-purity collagen peptide powder (large molecular weight chondroitin sulfate) extracted from sharks is broken down into small molecular weight chondroitin sulfate through modern enzymatic hydrolysis, with a molecular weight <1000 Da.

[0045] S3. Premix: The powdered materials of 15% yeast extract in the food-attracting substance and 10% chondroitin sulfate, 1% collagen, 2% turmeric and 1% black pepper extract in the functional ingredients are stirred and premixed to obtain the premix.

[0046] S4. Mixing: Add 10% dextrin (selected from supramolecular complex cyclodextrin inclusion complex), 5% corn starch (carrier), 3% water, 7% meat products and 8% hydrolysate (food attractant), 1.3% green-lipped mussel, 1.2% krill, 1.8% oyster, 0.7% frankincense, and 1% hyaluronic acid (functional ingredients) to the premix and stir until homogeneous at 350 rpm for 3 minutes.

[0047] S5. Drying and Discharging: After drying the semi-finished product obtained after mixing, cut it into equal portions and discharge it. The drying temperature is 75℃ and the time is 22min.

[0048] Example 5

[0049] A pet bone and joint protectant based on chondroitin LPM penetration comprises the following components: carrier, water, palatability-enhancing ingredient, and functional ingredient, wherein the components of the material by weight percentage are as follows: carrier 1%, water 3%, palatability-enhancing ingredient 1%, and functional ingredient 1%.

[0050] S1. Preparation: Prepare sufficient raw materials for carriers, palatability-enhancing ingredients, and some functional ingredients;

[0051] S2. Raw material preparation: High-purity collagen peptide powder (large molecular weight chondroitin sulfate) extracted from sharks is broken down into small molecular weight chondroitin sulfate through modern enzymatic hydrolysis, with a molecular weight <1000 Da.

[0052] S3, Premix: The powdered materials containing 0.2% yeast extract from the food-attracting substances and 0.3% chondroitin sulfate, 0.08% collagen, 0.07% turmeric, and 0.12% black pepper extract from the functional ingredients are stirred and premixed to obtain the premix.

[0053] S4. Mixing: Add the pretreated carrier consisting of 0.6% dextrin selected from supramolecular complex cyclodextrin inclusion complex, 0.4% corn starch, 3% water, and 0.4% meat products and their hydrolysates from the attractant, as well as 0.14% green-lipped mussels, 0.16% krill, 0.05% oysters, 0.02% frankincense, and 0.06% hyaluronic acid from the functional ingredients to the premix and stir until homogeneous at 300 rpm for 3 minutes.

[0054] S5. Drying and Discharging: After drying the semi-finished product obtained after mixing, cut it into equal portions and discharge it. The drying temperature is 75℃ and the time is 20min.

[0055] The pet osteoarthritis protectants based on chondroitin LPM penetration prepared in Examples 1-5 were tested on 30 dogs of similar physical condition and age for 8 weeks. The protectant samples prepared in Examples 1-5 were also tested on 6 dogs in a feeding experiment. The specific tests are shown in the table below:

[0056]

[0057] Table 1

[0058]

[0059]

[0060] Table 2

[0061]

[0062] Table 3

[0063] The pet osteoarthritis protectant samples prepared in Examples 1-5 above based on chondroitin LPM penetration were tested on 20 dogs of similar age with certain joint problems. The protectant samples prepared in Examples 1-5 were also tested on 4 dogs in a feeding experiment. The specific tests are shown in the table below:

[0064]

[0065] Table 4

[0066] Comparative Example 1

[0067] A pet bone and joint protectant based on chondroitin LPM penetration comprises the following components: carrier, water, palatability-enhancing ingredient, and functional ingredient, wherein the components of the material by weight percentage are as follows: carrier 30%, water 4%, palatability-enhancing ingredient 30%, and functional ingredient 30%.

[0068] S1. Preparation: Prepare sufficient raw materials for carriers, palatability-enhancing ingredients, and some functional ingredients;

[0069] S2. Raw material preparation: High-purity collagen peptide powder (high molecular weight chondroitin sulfate) is extracted from sharks;

[0070] S3. Premix: The powdered materials of 5% yeast extract from the food-attracting substance and 13% chondroitin sulfate, 2% collagen, 3% turmeric, and 1% black pepper extract from the functional ingredients are stirred and premixed to obtain the premix.

[0071] S4. Mixing: Add the carrier consisting of 15% ordinary dextrin and 15% corn starch, 4% water, 15% meat products and 10% hydrolysate from the attractant, and 2% green-lipped mussel, 3% krill, 2% oyster, 2% frankincense, and 2% hyaluronic acid from the functional ingredients to the premix and stir until well mixed at 500 rpm for 5 minutes.

[0072] S5. Drying and Discharging: After drying the semi-finished product obtained after mixing, cut it into equal portions and discharge it. The drying temperature is 90℃ and the time is 30min.

[0073] Comparative Example 2

[0074] A pet bone and joint protectant based on chondroitin LPM penetration comprises the following components: carrier, water, palatability-enhancing ingredient, and functional ingredient, wherein the components of the material by weight percentage are as follows: carrier 5%, water 4%, palatability-enhancing ingredient 25%, and functional ingredient 25%.

[0075] S1. Preparation: Prepare sufficient raw materials for carriers, palatability-enhancing ingredients, and some functional ingredients;

[0076] S2. Raw material preparation: High-purity collagen peptide powder (high molecular weight chondroitin sulfate) is extracted from sharks;

[0077] S3, Premix: The powdered materials of 3% yeast extract in the food-attracting substance and 10% chondroitin sulfate, 1% collagen, 3% turmeric, and 2% black pepper extract in the functional ingredients are stirred and premixed to obtain the premix.

[0078] S4. Mixing: Add the carrier consisting of 3% ordinary dextrin and 2% corn starch, 4% water, 15% meat products and 7% hydrolysate from the attractant, and 2% green-lipped mussel, 1.5% krill, 2% oyster, 2% frankincense, and 1.5% hyaluronic acid from the functional ingredients to the premix and stir until well mixed at 400 rpm for 4 minutes.

[0079] S5. Drying and Discharging: The semi-finished product obtained after mixing is dried and then cut into equal portions for discharge. The drying temperature is 85℃ and the time is 27 minutes.

[0080] The pet osteoarthritis protectants prepared in Comparative Examples 1-2 above, based on traditional macromolecular chondroitin sulfate and ordinary dextrin, were tested on 12 dogs of similar physical condition and age for 8 weeks. The protectant samples prepared in Examples 1-2 were tested on 6 dogs in four groups of feeding experiments. The specific tests are shown in the table below:

[0081]

[0082] Table 5

[0083]

[0084] Table 6

[0085]

[0086] Table 7

[0087] The pet osteoarthritis protectant samples prepared from dextrin based on small molecule chondroitin sulfate and supramolecular complex cyclodextrin inclusion complex in Examples 1-2 and the pet osteoarthritis protectant samples prepared from conventional large molecule chondroitin sulfate and ordinary dextrin in Comparative Examples 1-2 were tested for their anti-acid effects on 16 dogs of similar physical condition and age. Each protectant sample was tested on 4 dogs. The specific test results are shown in the table below:

[0088]

[0089] Table 8

[0090] A comparison of the test results from Examples 1-5 and Comparative Examples 1-2 shows that, compared to the use of conventional macromolecular chondroitin sulfate and ordinary dextrin in Comparative Examples 1-2, the protective agent designed in this product (Examples 1-5) resulted in a higher rate of symptom improvement in pets' bone and joint conditions within 0-8 weeks, with better efficacy. Furthermore, serum 1L-1β analysis revealed a more significant reduction in bone and joint inflammation in pets consuming this product. Additionally, the absorption rate and penetration rate of the LPM product were significantly higher after 1-5 days of consumption. Based on experiments on absorption rate and activity rate, the product showed significant improvements in absorption rate, penetration rate, and activity rate. Furthermore, the gastric acid resistance test results indicated that this protectant was more effective in resisting gastric acid after administration. This is due to the molecular weight combination between chondroitin sulfate (small molecule) and the supramolecular complex cyclodextrin inclusion complex, which effectively enhances gastric acid resistance, thereby increasing product penetration rate and activity rate, and improving absorption rate. Additionally, the pet bone and joint protectant samples prepared in Examples 1-5 showed a gradual decrease in adverse events and incidence during 0-6 weeks of feeding, indicating a decrease in incidence over time and demonstrating good applicability.

[0091] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A pet osteoarthritis protectant based on chondroitin LPM penetration, comprising the following components: carrier, water, palatability-enhancing ingredients, and functional ingredients, characterized in that: The material comprises the following components by weight percentage: carrier 1%-30%, water 3-4%, palatability-enhancing ingredients 1%-30%, and functional ingredients 1-30%.

2. The pet osteoarthritis protectant based on chondroitin LPM penetration according to claim 1, characterized in that: The carrier is composed of dextrin and corn starch.

3. The pet osteoarthritis protectant based on chondroitin LPM penetration according to claim 1, characterized in that: The palatability-enhancing ingredient consists of meat products, meat product hydrolysate, and yeast extract.

4. The pet osteoarthritis protectant based on chondroitin LPM penetration according to claim 1, characterized in that: The functional ingredients consist of a mixture of chondroitin sulfate, green-lipped mussel, krill, oyster, collagen, turmeric, frankincense, black pepper extract, and hyaluronic acid.

5. The pet osteoarthritis protectant based on chondroitin LPM penetration according to claim 4, characterized in that: The chondroitin sulfate is a small molecule chondroitin sulfate, which is produced by the enzymatic degradation of large molecule chondroitin sulfate. The molecular weight of the small molecule chondroitin sulfate is <1000 Da, and the raw material for the small molecule chondroitin sulfate is selected from any one or more of shark, cow and chicken.

6. The pet osteoarthritis protectant based on chondroitin LPM penetration according to claim 2, characterized in that: The paste is selected from supramolecular complex cyclodextrin inclusion complexes.

7. A method for preparing a pet osteoarthritis protectant based on chondroitin LPM penetration according to any one of claims 1-6, characterized in that: Includes the following steps: S1. Preparation: Prepare sufficient raw materials for carriers, palatability-enhancing ingredients, and some functional ingredients; S2. Raw material preparation: High-purity collagen peptide powder (large molecular weight chondroitin sulfate) is extracted from any one or more of shark, cow and chicken, and then broken down into small molecular weight chondroitin sulfate through modern enzymatic hydrolysis, with a molecular weight <1000Da. S3. Premix: The powdered yeast extract from the food-attracting substance and the small molecule chondroitin sulfate, collagen, turmeric, and black pepper extract from the functional ingredients are stirred and premixed to obtain the premix. S4. Mixing: Add all the pretreated carriers, water, meat products and their hydrolysates from the attractant, and green-lipped mussels, krill, oysters, frankincense, and hyaluronic acid from the functional ingredients to the premix and stir until well mixed. The stirring speed is 300-500 rpm and the stirring time is 3-5 minutes. S5. Drying and Discharging: After drying the semi-finished product obtained after mixing, cut it into equal portions and discharge it. The drying temperature is 75-90℃ and the time is 20-30 minutes.

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