An anti-aging composition and uses thereof, a skin care product

Abstract

The application belongs to the technical field of cosmetics and discloses an anti-aging composition and purposes, skin care products, which comprise royal jelly polypeptide, Dendrobium candidum stem extract solution, ginsenoside solution and copper gluconate; the mass ratio of the royal jelly polypeptide, the Dendrobium candidum stem extract solution, the ginsenoside solution and the copper gluconate is 3-5:18-22:18-22:4-6; the application provides a significant improvement in the expression amount of collagen through the cooperation between the royal jelly polypeptide, the Dendrobium candidum stem extract solution, the ginsenoside solution and the copper gluconate, and then the content of elastin is improved; on the other hand, the synergistic arrangement between the above raw materials can reduce the generation of MDA of oxidative damage cells and provide good antioxidant capacity.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of cosmetics, and discloses an anti-aging composition and purposes and skin care products thereof. BACKGROUND

[0002] Skin is wrapped on the body surface of the human body and directly contacts with the external environment, and is an organ with the functions of protection, excretion, regulation of body temperature and feeling of external stimulation, and is the largest organ among human body organs, and the health of the skin is also very important to the human body; with the increase of age and the influence of the external environment, the metabolism of human skin slows down, the moisturizing factor in the dermis decreases, the function of the elastic fiber and collagen fiber in the dermis decreases, the skin gradually presents aging and the aging damage is aggravated, the protection ability and the regulation ability of the skin to the organism decrease, therefore, the skin cannot adapt to the changes of the internal and external environment, the skin tension and elasticity weaken, and the aging characteristics such as wrinkles and dull skin appear.

[0003] Everyone has a love for beauty, and people's demand for delaying skin aging, improving wrinkles, relaxation and other aging skin problems is increasingly urgent. At present, the anti-aging skin care products on the market mainly play a role through the ways of antioxidant, promotion of collagen generation, moisturizing repair and the like. Antioxidant components such as vitamin C, vitamin E, nicotinamide and the like can effectively scavenge free radicals and delay skin oxidative damage, but such components have poor stability and are easily inactivated by environmental factors such as light and air; and in the face of long-term accumulated oxidative stress damage, the effect of a single antioxidant component is limited. Peptide components can stimulate fibroblasts to synthesize collagen, but their penetration efficiency is low, most of which can only act on the surface layer of the skin, and it is difficult to penetrate into the dermis layer to solve the problem of collagen loss from the cellular level. And moisturizing repair components such as hyaluronic acid, ceramide and the like can temporarily improve the dry and rough condition of the skin, but cannot inhibit collagen degradation caused by the increase of matrix metalloproteinase (MMP) activity from the root, and it is difficult to effectively intervene in the process of skin aging. Moreover, some anti-aging skin care products are easy to irritate the skin, may cause irreversible damage to the skin, and have limited effect.

[0004] Therefore, the problem to be solved by the present application is to provide a safe and efficient anti-aging composition. SUMMARY

[0005] The purpose of the present application is to provide an anti-aging composition, which comprises royal jelly polypeptide, dendrobium stem extract solution, ginsenoside solution and copper gluconate, the above components are compounded, have obvious synergistic effect, and can play high anti-aging effect.

[0006] Meanwhile, the present application also provides purposes of the anti-aging composition and skin care products.

[0007] To achieve the above object, the present application provides the following technical scheme.

[0008] An anti-aging composition comprises royal jelly polypeptide, Dendrobium stem extract solution, ginsenoside solution and copper gluconate; the mass ratio of the royal jelly polypeptide, the Dendrobium stem extract solution, the ginsenoside solution and the copper gluconate is 3-5:18-22:18-22:4-6.

[0009] Preferably, a synergist is further included, and the synergist is at least one selected from the group consisting of Inonotus obliquus extract and Fucus vesiculosus extract;

[0010] The mass ratio of the synergist to the royal jelly polypeptide, the Dendrobium stem extract solution, the ginsenoside solution and the copper gluconate is 4-6:3-5:18-22:18-22:4-6.

[0011] Preferably, the royal jelly polypeptide is a mixture of hydrolyzed royal jelly protein and malt dextrin, and the mass ratio of the hydrolyzed royal jelly protein to the malt dextrin is 1:8-10.

[0012] Preferably, the following components are included in terms of mass fraction:

[0013] Royal jelly polypeptide 3-5 parts;

[0014] Dendrobium stem extract solution 18-22 parts;

[0015] Ginsenoside solution 18-22 parts;

[0016] Copper gluconate 4-6 parts;

[0017] Water 40-50 parts;

[0018] Butanediol 4-6 parts;

[0019] 1,2-hexanediol 0.1-1 part;

[0020] p-hydroxyacetophenone 0.1-1 part.

[0021] In addition, the present application also discloses the use of the anti-aging composition in preparing a skin care product.

[0022] In addition, the present application also discloses a skin care product containing the anti-aging composition.

[0023] Preferably, the content of the anti-aging composition in the skin care product is 0.05wt%-10wt%.

[0024] Preferably, the skin care product is one of emulsion, cream, spray, essence, essential oil, mask and jelly.

[0025] Compared with the prior art, the present application has the following beneficial effects:

[0026] This application utilizes the combination of royal jelly peptides, Dendrobium officinale stem extract solution, ginsenoside solution, and copper gluconate to significantly enhance collagen expression, thereby increasing elastin content. Furthermore, the synergistic combination of these ingredients reduces the generation of MDA in oxidatively damaged cells, thus providing excellent antioxidant capacity. Attached Figure Description

[0027] Figure 1 This is a schematic diagram showing the test results of the relative expression level of type III collagen gene.

[0028] Figure 2 This is a schematic diagram of the elastin content test results;

[0029] Figure 3 This is a schematic diagram of the test results for the upregulation rate of elastin content. Detailed Implementation

[0030] The technical solution of this application will be clearly and completely described below with reference to the embodiments thereof. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0031] Product Information:

[0032] Royal jelly polypeptide: purchased from Guangzhou Jikemeichuang Life Science Co., Ltd.;

[0033] Dendrobium officinale stem extract solution: purchased from Guangzhou Jikemeichuang Life Science Co., Ltd.;

[0034] Ginsenoside solution: purchased from Guangzhou Jikemeichuang Life Science Co., Ltd.;

[0035] Copper gluconate: purchased from Zhengzhou Ruipu Bioengineering Co., Ltd.;

[0036] Inonotus obliquus extract: purchased from Guangzhou Rancui Chemical Co., Ltd.;

[0037] Fucus vesiculosus extract: purchased from Guangzhou Jiaye Biotechnology Co., Ltd.

[0038] The preparation method of the anti-aging composition is as follows:

[0039] The solution was obtained by mixing royal jelly polypeptide, copper gluconate, p-hydroxyacetophenone, 1,2-hexanediol, ginsenoside solution, and Dendrobium officinale stem extract solution with pure water at room temperature and then filtering.

[0040] (Note that the following examples and comparative examples are prepared by the above method, unless otherwise specified.)

[0041] Examples 1-3

[0042] An anti-aging composition, the formulation of which is shown in Table 1.

[0043] Table 1: Formulation table of anti-aging composition of Examples 1-3 (mass parts)

[0044] Ingredients Example 1 Example 2 Example 3 Royal jelly polypeptide 4 3 5 Dendrobium stem extract solution 20 22 18 Ginsenoside solution 20 18 22 Copper gluconate 5 6 4 Water 45 40 50 Butylene glycol 5 6 4 1,2 Hexanediol 0.5 1 0.1 p-Hydroxyacetophenone 0.5 1 0.1

[0045] Example 4

[0046] The same as Example 1, except that the royal jelly polypeptide is replaced by hydrolyzed royal jelly protein.

[0047] Example 5

[0048] The same as Example 1, except that the royal jelly polypeptide is replaced by malt dextrin.

[0049] Examples 6-8

[0050] An anti-aging composition, the formulation of which is shown in Table 2.

[0051] Table 2: Formulation table of anti-aging composition of Examples 6-8 (mass parts)

[0052] Ingredients Example 6 Example 7 Example 8 Royal jelly polypeptide 4 4 4 Dendrobium stem extract solution 20 20 20 Ginsenoside solution 20 20 20 Copper gluconate 5 5 5 Inonotus obliquus extract 5 0 2.5 Chondrus crispus extract 0 5 2.5 Water 45 45 45 Butylene glycol 5 5 5 1,2 Hexanediol 0.5 0.5 0.5 p-Hydroxyacetophenone 0.5 0.5 0.5

[0053] Comparative Examples 1-12

[0054] A composition, the formulation of which is shown in Table 3:

[0055] Table 3: Formulation table of Comparative Examples 1-12 (mass parts)

[0056] Ingredients Royal jelly polypeptide Dendrobium stem extract solution Ginsenoside solution Copper gluconate Zinc gluconate Chlorophyllin copper Water Butylene glycol 1,2 Hexanediol p-Hydroxyacetophenone Comparative Example 1 0 22 22 5 0 0 45 5 0.5 0.5 Comparative Example 2 8 0 36 5 0 0 45 5 0.5 0.5 Comparative Example 3 8 36 0 5 0 0 45 5 0.5 0.5 Comparative Example 4 0 22 22 0 5 0 45 5 0.5 0.5 Comparative Example 5 8 0 36 0 5 0 45 5 0.5 0.5 Comparative Example 6 8 36 0 0 5 0 45 5 0.5 0.5 Comparative Example 7 4 20 20 0 5 0 45 5 0.5 0.5 Comparative Example 8 0 22 22 0 0 5 45 5 0.5 0.5 Comparative Example 9 8 0 36 0 0 5 45 5 0.5 0.5 Comparative Example 10 8 36 0 0 0 5 45 5 0.5 0.5 Comparative Example 11 4 20 20 0 0 5 45 5 0.5 0.5

[0057] Performance test:

[0058] 1. Relative expression of cell malondialdehyde (MDA)

[0059] 1.1 Malondialdehyde (MDA) is a lipid peroxidation end product formed after cell peroxidation. MDA content is an important parameter reflecting the potential antioxidant capacity of the body, which can reflect the rate and intensity of lipid peroxidation in the body, and also indirectly reflect the degree of peroxidation damage to tissues. Accumulation of MDA can lead to cross-linking and precipitation of biological macromolecules such as proteins and nucleic acids, forming insoluble lipofuscin, thereby accelerating the aging of the body. Based on the detection of MDA levels in oxidative stress damaged cell models, the antioxidant capacity of the test substance can be explored, and its anti-aging effect can be evaluated.

[0060] 1.2 Materials and equipment

[0061] 1.2.1 Cells: HaCaT cells (ATCC CRL-2404, human immortalized keratinocytes, American Type Culture Collection)

[0062] 1.2.2 Reagents: Vitamin C (ascorbic acid, purity ≥98%, source leaf), MDA detection kit (Nanjing Jiancheng, A003-4-1)

[0063] 1.2.3 Equipment: CO2 incubator (Thermo, 150I), clean bench (Sunan Antai, SW-CJ-1F), Olympus microscope (CK53), multifunctional enzyme label instrument (TECAN, SPARK)

[0064] 1.3 Test sample processing

[0065] Dilute the test sample with culture medium to a concentration of 2wt%, filter it through a 0.22μm microporous filter, and collect the filtrate as the mother liquor.

[0066] 1.4 Experimental method

[0067] 1.4.1 Cell viability test

[0068] HaCaT cells were resuspended after digestion and inoculated in a 96-well plate. After 24h, the culture medium was aspirated and complete culture medium containing 2wt% of the test sample (compositions prepared in the examples and comparative examples as test samples) was added. After 24h, MTT method was used to detect OD570nm and OD630nm, and the effect of the test sample on the growth of HaCaT cells was analyzed.

[0069] 1.4.2 Relative expression amount of cell MDA

[0070] After the HaCaT cells were digested and resuspended, they were inoculated into multiple 6-well plates. After the cells were attached for 24 h, a culture medium containing 2 wt% of the compositions prepared in different examples and comparative examples was used as the test sample. The positive control group (PC) was pre-protected for 24 h by adding a culture medium containing 10 μg / mL ascorbic acid, and the blank control group (Control) was only added with the culture medium. After the treatment time ended, the old culture medium was discarded, a small amount of PBS was added to each well, and ultraviolet UVB irradiation was performed at an irradiation dose of 48 mJ / cm2. After the irradiation ended, the PBS in the culture plate was discarded, and the corresponding culture medium was added to each group for continued culture for 24 h. The cells were collected, and the lipid oxidation (MDA) detection kit was used for detection according to the instructions. The MDA content of the cells was calculated, and the formula is shown as formula 1:

[0071] Formula 1

[0072] C standard: standard concentration, 10 nmol / mL;

[0073] Cpr: sample protein concentration, mg prot mL (prot refers to protein), determined by BCA method.

[0074] The test results are shown in Table 4:

[0075] Group MDA relative expression amount Group MDA relative expression amount Example 1 -24% Comparative Example 4 -11% Example 2 -22% Comparative Example 5 -7% Example 3 -21% Comparative Example 6 -11% Example 4 -20% Comparative Example 7 -13% Example 5 -18% Comparative Example 8 -8% Example 6 -27% Comparative Example 9 -7% Example 7 -25% Comparative Example 10 -10% Example 8 -32% Comparative Example 11 -9% Comparative Example 1 -10% Blank group / Comparative Example 2 -8% Positive control -36% Comparative Example 3 -10%

[0076] Table 4: Test results of relative expression amount of cell malondialdehyde (MDA)

[0077] Result analysis

[0078] 1. As can be seen from Examples 1-3, when the addition amount of the raw materials in the composition is adjusted by a small amount, the antioxidant capacity of Examples 1-3 fluctuates, but the fluctuation range is relatively small;

[0079] Further observation of Example 4 shows that when the royal jelly protein hydrolysate is used to replace the royal jelly polypeptide, the relative expression amount of MDA of the composition prepared in Example 4 is increased by 4% compared with Example 1. It can be seen that the malt dextrin in the present case has a certain antioxidant capacity. Further observation of Example 5 shows that when the royal jelly polypeptide is replaced by malt dextrin, the antioxidant capacity of the composition is further reduced. It can be seen that the antioxidant performance of the royal jelly polypeptide in the present case comes from both the royal jelly protein hydrolysate and the malt dextrin, and more importantly, when the royal jelly protein hydrolysate or the malt dextrin is used alone, the antioxidant capacity of Examples 4-5 is lower than that of Example 1. It can be seen that there may be a synergistic effect between the royal jelly protein hydrolysate and the malt dextrin to improve the antioxidant capacity of the composition;

[0080] 2. As can be seen from Examples 1 and 6-7, after adding Inonotus obliquus extract or Fucus vesiculosus extract to Example 1, the relative expression level of MDA in Examples 6-7 was further inhibited, indicating that the antioxidant capacity of the composition was further enhanced. Further observation of Example 8 shows that when Inonotus obliquus extract and Fucus vesiculosus extract were added to the composition at the same time, the MDA inhibition capacity of Example 8 was significantly better than that of either Example 6-7. Therefore, it can be seen that there may be a synergistic effect between Inonotus obliquus extract and Fucus vesiculosus extract to further enhance the antioxidant capacity of the composition.

[0081] 3. As can be seen from Example 1 and Comparative Examples 1-3, when any one of the royal jelly peptides, Dendrobium officinale stem extract solution, and ginsenoside solution is missing from the composition, the antioxidant capacity of Comparative Examples 1-3 is significantly reduced compared to Example 1. This suggests that there may be a synergistic effect among royal jelly peptides, Dendrobium officinale stem extract solution, and ginsenoside solution to increase the antioxidant capacity of the composition.

[0082] Further observation of Comparative Examples 4-7 reveals that Comparative Example 4-6 differs from Comparative Examples 1-3 only in that copper gluconate is replaced with zinc gluconate. Furthermore, Comparative Example 4-6 shows that its antioxidant capacity is quite similar to that of Comparative Examples 1-3, and in some cases it is even superior to that of Comparative Examples 1-3. Therefore, it is evident that the antioxidant capacity of zinc gluconate may be slightly better than that of copper gluconate.

[0083] However, further observation of Comparative Example 7 shows that, given the known synergistic effect among royal jelly polypeptide, Dendrobium officinale stem extract solution, and ginsenoside solution, the improvement trend of Comparative Example 7 compared to Comparative Examples 4-6 is not obvious.

[0084] In contrast, Example 1 showed a significant improvement in antioxidant capacity compared to Comparative Examples 1-3. This indicates that when royal jelly peptides, Dendrobium officinale stem extract solution, and ginsenoside solution coexist, they have a synergistic effect. Furthermore, when copper gluconate is used with all three, this synergistic effect may be further amplified, while zinc gluconate, which is also a gluconate, cannot achieve the same effect.

[0085] Further experiments were conducted using copper chlorophyllin. When copper chlorophyllin was used to replace copper gluconate in Comparative Example 8-10 compared to Comparative Example 1-3, the antioxidant capacity of Comparative Example 8-10 was slightly inferior to that of Comparative Example 1-3. This suggests that the antioxidant capacity of copper chlorophyllin may be slightly weaker than that of copper gluconate.

[0086] Further observation of Comparative Example 11, under the premise that there may be a synergistic effect between royal jelly polypeptide, dendrobium stem extract solution, ginsenoside solution, Comparative Example 11 is almost not seen obvious improvement compared with Comparative Examples 8-10, it can be seen that when copper salt is used to replace copper gluconate, copper salt cannot directly amplify the synergistic effect between royal jelly polypeptide, dendrobium stem extract solution, ginsenoside solution.

[0087] 2. Relative expression amount of collagen type III gene (qPCR)

[0088] 2.1 Experimental principle

[0089] Ultraviolet radiation can lead to the continuous increase of matrix metalloproteinase (MMPs) expression and activity in the skin, degrading elastin and collagen in the extracellular matrix of skin cells, and accelerating the process of skin aging. Collagen type III is the main structural protein in the dermis,

[0090] Synthesized in fibroblasts, its structure is a fine reticular fiber, which together with collagen type I (thick bundle) forms a skin support network. Normal skin is basically dominated by collagen types I and III, and their functions are closely related to skin elasticity and repair capacity.

[0091] 2.2 Equipment and materials

[0092] 2.2.1 Equipment: Multifunctional enzyme marker (TECAN, SPARK), carbon dioxide incubator (Thermo), fluorescence quantitative PCR instrument (Bole, CFXConnectOticsodule), PCR amplifier (Langji, A300)

[0093] 2.2.2 Cell line: HDF (human dermal fibroblasts, Sibcro (Shanghai) Biotechnology Co., Ltd.)

[0094] 2.2.3 Reagents: Transforming growth factor β1 protein (Sigma), RNA rapid extraction kit (Meiji), qPCR Mix kit (SYBR Green)

[0095] 2.3 Experimental method

[0096] 2.3.1 Cell activity test

[0097] Take HDF cells to plate 96-well plates, discard the culture medium after 24 hours, add complete culture medium containing different concentrations of test samples (compositions prepared in Example 1), detect OD570nm and OD630nm by MTT method after 24 hours, and analyze the effect of test samples on HDF cell growth by t test, and select appropriate concentration for efficacy test.

[0098] 2.3.2 Cellular Type III Collagen Expression Assay

[0099] Cells were diluted with cell culture medium to the seeding density (55%–65% confluence reached 24 h post-seeding) and seeded into 24-well plates, with 500 μL per well. After seeding, the plates were incubated in a CO2 incubator for 24 h ± 2 h. The culture medium in the 24-well plates was discarded, and 100 μL of PBS was added to each well to cover the cells. The plates were then irradiated with UVB at 48 mJ / cm². The blank control group was covered with aluminum foil to prevent cell exposure. After UVB irradiation, the cells were washed once with PBS, and the drug administration was carried out according to the experimental design in Table 5. The sample groups were added to culture medium containing the test substance, the positive control group was added to culture medium containing the positive control, and the blank / solvent control wells were added to cell culture medium and cultured for another 48 h.

[0100] Table 5: Experimental design of UVA-fibroblast model (qPCR)

[0101]

[0102] After cell culture, total mRNA was extracted, quantified, and cDNA was obtained by reverse transcription for Real-Time PCR. The relative expression level of Collagentype III genes was analyzed using the 2-ΔΔCT method. The average Ct value of three replicates for each gene in each cDNA sample was used as the amplification result. The amplification level of GAPDH gene was used as the internal reference gene. The gene cycle threshold ΔCt was calculated as Ct = gene - CtGAPDH, and the relative gene expression level ΔΔCt = ΔCt test substance - ΔCt blank control. The ratio of expression levels between the test substance group and the control group was calculated using 2-ΔΔCT analysis.

[0103] Results Analysis: The test results are as follows: Figure 1 As shown, the expression level of Collagentype III gene in skin fibroblasts was significantly decreased after UVB irradiation oxidative damage (p<0.01). In the positive control group (PC), treatment with 100 ng / mTGF-β1 significantly upregulated Collagentype III gene expression compared to the model control group (NC) (p<0.01), demonstrating the effectiveness of the experimental system.

[0104] Treatment with 0.20% of the sample prepared in Example 1 (Sanmutong) significantly increased the expression of the collagen type III gene in cells compared with the model control group (NC). The results indicate that Sanmutong has an inhibitory effect on the decrease in type III collagen gene expression in human skin fibroblasts damaged by UVB irradiation.

[0105] 3. Elastin content assay (ELISA)

[0106] 3.1 Experimental Principle

[0107] UV stimulation can lead to the continuous increase of matrix metalloproteinase (MMPs) expression and activity in the skin, degrading elastin and collagen in the extracellular matrix of skin cells, and accelerating the process of skin aging. By UVA irradiation of human fibroblasts to construct an oxidative damage model, the change of elastin content after treatment of the test substance can be determined to evaluate the ability of the test substance to resist photoaging.

[0108] 3.2 Equipment and materials

[0109] 3.2.1 Equipment: CO2 incubator (Thermo, 150I), super clean bench (Su Nengantai, SW-CJ-1F), Olympus microscope (CK53), multifunctional enzyme label instrument (TECAN, SPARK);

[0110] 3.2.2 Cell line: HSF (human skin fibroblasts, Shanghai Bolson Biotechnology Co., Ltd.);

[0111] 3.2.3 Reagents: TGF-β1 was purchased from PEPROTECH company, Human Elastin Elisa detection kit was purchased from Huawmei Biological Company;

[0112] 3.3 Sample processing

[0113] The test substance was filtered through a 0.22 μm filter membrane and diluted with cell culture medium to the required concentration.

[0114] 3.4 Experimental method

[0115] 3.4.1 Cell preparation

[0116] The cells were cultured at 37℃, 5% CO2, and the cell density was controlled to contain 1.0×10 4 ~ 5.0×10 5 cells per 1 mL after subculture, and used for biological activity determination 24-36 h later.

[0117] 3.4.2 HSF cell activity test

[0118] After 24 h of culture, the medium was replaced with a medium containing different concentrations of the test sample, and the OD450nm value was detected by CCK-8 method 24 h later to analyze the effect of the test sample on the tolerance of HSF cells.

[0119] 3.4.3 Efficacy detection

[0120] Dilute cells to the seeding density using cell culture medium (confluence reaches 45%–60% after 24 hours) and seed into 24-well plates, 500 μL per well. After seeding, incubate in a CO2 incubator for 24 hours ± 2 hours. Discard the culture medium in the 24-well plates and perform drug administration according to the experimental design in Table 6. Add culture medium containing the test substance (the composition prepared in Example 1 (Sanmutong)) to the test wells, add culture medium containing the positive control to the positive control wells, and add cell culture medium to the blank / solvent control wells, 500 μL per well, for pre-protection drug administration for 24 hours. Remove the culture medium, add 100 μL of PBS to each well to cover the cells, and use 10 J / cm² water. 2 The cells were irradiated with UVA, while the blank control group was covered with aluminum foil to prevent cell exposure. After UVA irradiation, the cells were washed once with PBS, and then cultured for another 48 hours with the prepared solutions for each group.

[0121] Table 6: Experimental Design of UVA-Fibroblast Model (ELISA)

[0122]

[0123] After incubation, the cell culture supernatant was collected into sterile centrifuge tubes and stored at -80°C. Elastin was detected according to the instructions for use of the human elastase-linked immunosorbent assay kit.

[0124] 5 Result Calculation

[0125] The regression equation for the standard curve was calculated using the concentration and OD450 value of the standard. The OD450 value of the sample was then substituted into the equation to calculate the elastin content of the sample. The average value of the three replicates for each group was taken as the final elastin result. The elastin upregulation rate was calculated according to Equation 2.

[0126] Formula 2

[0127] In the formula, T represents the average elastin content of the test substance;

[0128] C represents the average elastin content in the blank / negative control group.

[0129] The experimental results are shown in Table 7:

[0130] Table 7: Results of Elastin Detection

[0131]

[0132] Results analysis: The test results are shown in Table 7. Figure 2-3As shown, the elastin of skin fibroblasts after UVA irradiation oxidative damage appeared degradation, and the content decreased significantly. Compared with the model control group (NC), the content of elastin in the positive control group (PC) treated with 100 ng / mL TGF-β1 increased significantly (p<0.01), which proved that the test system was effective.

[0133] Compared with the model control group (NC), the content of elastin in the skin fibroblasts treated with 0.1% and 0.2% Sanmuto after UVA irradiation oxidative damage increased significantly (p<0.01), and the up-regulation rates of elastin content were 9.93% and 18.65%, respectively.

[0134] The experimental results showed that 0.1%-0.2% Sanmuto had inhibitory effect on the degradation and loss of elastin of human skin fibroblasts after UVA irradiation oxidative damage.

[0135] It is apparent for those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, but can be implemented in other concrete forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be considered in all aspects as illustrative and not restrictive, and the scope of the present application is defined by the appended claims rather than the above description, and it is intended to embrace all changes falling within the meaning and scope of the equivalent elements of the claims. Any reference signs in the claims should not be considered as limiting the claims involved.

Claims

1. An anti-aging composition characterized in that, The composition comprises royal jelly polypeptide, Dendrobium stem extract solution, ginsenoside solution and copper gluconate; the mass ratio of the royal jelly polypeptide, the Dendrobium stem extract solution, the ginsenoside solution and the copper gluconate is 3-5:18-22:18-22:4-6; The composition further comprises a synergist selected from at least one of chaga extract and fucus extract; The mass ratio of the synergist to the royal jelly polypeptide, the Dendrobium stem extract solution, the ginsenoside solution and the copper gluconate is 4-6:3-5:18-22:18-22:4-6.

2. Anti-aging composition according to claim 1, characterized in that, The royal jelly polypeptide is a mixture of hydrolyzed royal jelly protein and malt dextrin, and the mass ratio of the hydrolyzed royal jelly protein to the malt dextrin is 1:8-10.

3. The anti-aging composition according to claim 1, characterized in that, The composition comprises the following components by mass fraction: Royal jelly polypeptide 3-5 parts; Dendrobium stem extract solution 18-22 parts; Ginsenoside solution 18-22 parts; Copper gluconate 4-6 parts; Water 40-50 parts; Butylene glycol 4-6 parts; 1,2-hexanediol 0.1-1 part; p-hydroxyacetophenone 0.1-1 part.

4. Use of the anti-aging composition according to any one of claims 1-3 for preparing a skin care product.

5. A skin care product, characterized by, The skin care product contains the anti-aging composition according to any one of claims 1-4.

6. The skin care product of claim 5, wherein, The content of the anti-aging composition in the skin care product is 0.05-10 wt%.

7. The skincare product according to claim 5, characterized in that, The skin care product is one of emulsion, cream, spray, serum, essential oil, mask and jelly.

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