Chinese herbal medicine three-color strip toothpaste and preparation method thereof

Abstract

The invention belongs to the technical field of daily chemicals, and particularly relates to Chinese herbal medicine three-color strip toothpaste and a preparation method thereof. The Chinese herbal medicine three-color strip toothpaste comprises a faint yellow paste body, a green paste body and a blue paste body, the faint yellow paste body comprises, by mass, 0.1%-2% of chitosan quaternary ammonium salt and 0.1%-1% of an ovateleaf holly bark extract, and the green paste body comprises, by mass, 0.002%-0.15% of sodium copper chlorophyllin and 0.1%-1% of the ovateleaf holly bark extract. The blue paste is prepared from the following components in percentage by mass: 0.03 to 0.15 percent of guaiazulene sodium sulfonate and 0.1 to 1 percent of ovateleaf holly bark extract. According to the Chinese herbal medicine three-color-strip toothpaste and the preparation method thereof provided by the invention, the three-color-strip toothpaste is obtained under the condition that exogenous pigments are not added; the toothpaste can achieve the compound effects of freshening breath, inhibiting dental plaque and oral pathogenic bacteria, resisting inflammation, diminishing swelling, resisting allergy, relieving gingivitis and promoting healing and repairing of recurrent oral ulceration, can promote enamel remineralization and pore repairing, and is high in effect synergy, paste stability and use safety.

Description

Technical Field

[0001] This application belongs to the technical field of daily chemical products, specifically relating to a three-color striped toothpaste made with traditional Chinese medicine and its preparation method. Background Technology

[0002] With the continuous advancement of toothpaste production technology and the constant improvement of people's living standards, more and more consumers are shifting their focus to oral health and the aesthetics of their teeth. The global oral care market is currently showing a significant trend towards functionalization and naturalization. Consumer demand for multi-functional herbal toothpaste continues to grow. Concerns about chemically synthesized ingredients and a preference for herbal care are driving the upgrade of herbal toothpaste from its traditional partial effects of "anti-inflammatory," "antibacterial," "hemostatic," and "breath freshening" to a composite effect of "anti-inflammatory, antibacterial, hemostatic, breath freshening, and repairing."

[0003] Currently, most mainstream toothpaste products rely on a single ingredient to achieve specific functions (such as fluoride for cavity prevention and plant essential oils for antibacterial effects). These products have a single mechanism of action and lack a systematic solution for complex oral pathologies such as gingivitis combined with plaque buildup (for example, salicylic acid derivatives can only relieve gingival redness and swelling but cannot inhibit plaque biofilm formation, leading to recurrent inflammation). Furthermore, some products claiming to be multi-functional easily cause antagonistic effects between ingredients by simply mixing traditional Chinese medicine extracts, resulting in insufficient synergistic effects. In addition, while adding exogenous pigments to toothpaste can enhance visual appeal, indicate efficacy, and improve sensory experience, it also poses safety risks, stability issues, and the potential for long-term tooth discoloration that affects aesthetics.

[0004] In summary, there is an urgent need to provide a herbal toothpaste without added pigments to systematically solve the above problems and offer an innovative solution for oral health management. Summary of the Invention

[0005] The purpose of this application is to provide a herbal three-color striped toothpaste and its preparation method, so as to solve at least one of the above-mentioned technical problems.

[0006] To achieve the above objectives, this application adopts the following technical solution: This application provides a herbal three-color striped toothpaste, comprising a light yellow paste, a green paste, and a blue paste. The light yellow paste comprises 0.1% to 2% by mass of chitosan quaternary ammonium salt and 0.1% to 1% by mass of *Smilax china* extract. The green paste comprises 0.002% to 0.15% by mass of sodium copper chlorophyllin and 0.1% to 1% by mass of *Smilax china* extract. The blue paste comprises 0.03% to 0.15% by mass of sodium guaiacol and 0.1% to 1% by mass of *Smilax china* extract.

[0007] In some embodiments of this application, the mass ratio of the herbal tricolor strip toothpaste is light yellow paste: green paste: blue paste = 8-6:1-2.5:1-2.5.

[0008] In some embodiments of this application, the mass ratio of the herbal tricolor strip toothpaste is 7:1.5:1.5 (light yellow paste: green paste: blue paste) or 6:2:2 (light yellow paste: green paste: blue paste). In some embodiments of this application, the light yellow, green, and blue pastes each comprise 0.001%–0.05% by mass of rosemary extract, 0.005%–0.5% of dipotassium glycyrrhizate, and 0.005%–1.0% of arginine.

[0009] In some embodiments of this application, the pale yellow paste, green paste, and blue paste further comprise the following components by mass fraction: 45%–65% humectant, 16%–22% abrasive, 0.4%–1.4% thickener, 1.0%–3.0% surfactant, 0.5%–1.3% fragrance, 0.1%–0.22% sweetener, 0%–0.5% preservative, 0.1%–0.5% stabilizer, and the balance being water.

[0010] In some embodiments of this application, the moisturizer is one or more of sorbitol, polyethylene glycol (PEG400), glycerin, and propylene glycol.

[0011] In some embodiments of this application, the friction agent is one or more of hydrated silica, calcium carbonate, calcium phosphate, and aluminum hydroxide.

[0012] In some embodiments of this application, the thickener is one or more of sodium carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC), polyquaternium-22, carrageenan, xanthan gum, guar gum, carbomer, and xanthan gum.

[0013] In some embodiments of this application, the surfactant is one or more of sodium dodecyl sulfate (K12), sodium lauryl sulfate, lauryl glucoside, sodium lauroyl sarcosinate, betaine, sodium lauroyl glutamate, and sodium cocoyl methyl taurate.

[0014] In some embodiments of this application, the sweetener is one or more of sodium saccharin, sucralose, xylitol, erythritol, and steviol glycosides.

[0015] In some embodiments of this application, the preservative is one or more of sodium benzoate, parabens and their salts, and polyols.

[0016] In some embodiments of this application, the stabilizer is one or more of pyrophosphate and phosphate.

[0017] This application provides a method for preparing a herbal tricolor striped toothpaste, including the preparation step of a light yellow main paste:

[0018] 1. Weigh out deionized water (with a suitable amount left to rinse the container), and add in sequence 0.1%–0.22% sweetener, 0%–0.5% preservative, 0.1%–0.5% stabilizer, 0.005%–0.5% dipotassium glycyrrhizate and 0.005%–1.0% arginine by mass fraction, stir and dissolve evenly for later use;

[0019] 2. Weigh out a humectant with a mass fraction of 45%–65% for later use;

[0020] 3. Weigh out 16%–22% of abrasive, 0.4%–1.4% of thickener, 0.1%–2% of chitosan quaternary ammonium salt, and 0.1%–1% of strychnine extract by mass and premix them evenly in a stainless steel pot;

[0021] 4. Dissolve 0.01%–0.5% (by mass) of cooling agent and 0.005%–0.1% of rosemary extract in 0.5%–1.3% of fragrance for later use;

[0022] 5. Weigh out a surfactant with a mass fraction of 1.0% to 3.0% for later use;

[0023] 6. Add the sample from step 2 to the ointment preparation pot, then add the sample from step 1 and rinse the container with the remaining deionized water. Stir with a vacuum scraper for 3 minutes.

[0024] 7. Add the sample from step 3, turn on the scraper, stir until there is no obvious dry powder, turn on the vacuum, and start timing for 10 minutes when the vacuum reaches above -0.092MPa;

[0025] 8. Add the samples from steps 4 and 5 and turn on the vacuum scraper to stir for 30 minutes (vacuum not higher than -0.094 MPa);

[0026] 9. Turn off the scraper and stir. Degas under vacuum for more than 10 minutes until there are no obvious bubbles, and you will get a light yellow main paste.

[0027] In some embodiments of this application, the light yellow main paste preparation step 1 includes 0.1% sodium saccharin, 0.2% sodium benzoate, 0.3% sodium pyrophosphate, 0.2% dipotassium glycyrrhizate, and 0.8% arginine by mass fraction.

[0028] In some embodiments of this application, in step 2 of preparing the pale yellow main paste, 50% sorbitol, 3% PEG400, and 8% glycerin by mass fraction are weighed.

[0029] In some embodiments of this application, in step 3 of preparing the pale yellow main paste, 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, 0.5% chitosan quaternium salt, and 0.6% scutellaria extract by mass fraction are weighed.

[0030] In some embodiments of this application, in step 4 of preparing the pale yellow main paste, 0.1% cooling agent and 0.5% rosemary extract by mass fraction are dissolved in 1% fragrance.

[0031] In some embodiments of this application, in step 5 of preparing the pale yellow main paste, 2.0% K12, 0.5% sodium lauroyl sarcosinate, and 0.5% betaine by mass fraction are prepared for later use.

[0032] In some embodiments of this application, the preparation method includes a green excipient preparation step:

[0033] 1. Weigh out 0.5%–1% deionized sodium chlorophyllin and dissolve 0.002%–0.15% sodium copper chlorophyllin.

[0034] 2. Weigh out deionized water (with a suitable amount for rinsing the container), and add 0.1%–0.22% sweetener, 0%–0.5% preservative, 0.1%–0.5% stabilizer, 0.005%–0.5% dipotassium glycyrrhizate and 0.005%–1% arginine by mass fraction, stir and dissolve evenly for later use;

[0035] 3. Weigh out a humectant with a mass fraction of 45%–65% for later use;

[0036] 4. Weigh out 16%–22% friction agent, 0.4%–1.4% thickener, and 0.1%–1% sapote extract by mass and premix them evenly in a stainless steel pot;

[0037] 5. Dissolve 0.01%–0.5% (by mass) of cooling agent and 0.001%–0.1% of rosemary extract in 0.5%–1.3% of fragrance for later use;

[0038] 6. Weigh out a surfactant with a mass fraction of 1.0% to 3.0% for later use;

[0039] 7. Add the sample from step 3 to the ointment preparation pot, add the samples from steps 2 and 1, rinse the container with the remaining deionized water, and stir with a vacuum scraper for 3 minutes;

[0040] 8. Add the sample from step 4, turn on the scraper, stir until there is no obvious dry powder, turn on the vacuum, and start timing for 10 minutes when the vacuum reaches above -0.092MPa;

[0041] 9. Add the samples from steps 5 and 6 and turn on the vacuum scraper to stir for 30 minutes (vacuum not higher than -0.094 MPa);

[0042] 10. Turn off the scraper and stir. Degas under vacuum for more than 10 minutes until there are no obvious bubbles, and obtain the green auxiliary paste.

[0043] In some embodiments of this application, in step 1 of the preparation of the green excipient, 0.5% deionized sodium chlorophyllin is weighed and dissolved to dissolve 0.05% sodium copper chlorophyllin.

[0044] In some embodiments of this application, step 2 of the preparation of the green excipient contains 0.1% sodium saccharin, 0.3% sodium benzoate, 0.3% sodium pyrophosphate, 0.2% dipotassium glycyrrhizate, and 0.8% arginine by mass fraction.

[0045] In some embodiments of this application, step 3 of the preparation of the green excipient ointment weighs 50% sorbitol, 3% PEG400, and 8% glycerin by mass fraction.

[0046] In some embodiments of this application, step 4 of the preparation of the green excipient ointment weighs 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, and 0.4% safflower extract.

[0047] In some embodiments of this application, in step 5 of the preparation of the green excipient, 0.1% cooling agent and 0.05% rosemary extract by mass fraction are dissolved in 1% fragrance.

[0048] In some embodiments of this application, step 6 of the preparation of the green excipient contains 2.0% K12, 0.5% sodium lauroyl sarcosinate, and 0.5% betaine by mass fraction.

[0049] In some embodiments of this application, the preparation method includes a blue excipient preparation step:

[0050] 1. Dissolve 0.1% sodium guaiac sulfonate in 0.5% deionized water;

[0051] 2. Weigh out deionized water (with a suitable amount left to rinse the container), and add in sequence 0.1%–0.22% sweetener, 0%–0.5% preservative, 0.1%–0.5% stabilizer, 0.005%–0.5% dipotassium glycyrrhizate and 0.005%–1% arginine by mass fraction, stir to dissolve evenly and set aside.

[0052] 3. Weigh out a humectant with a concentration of 45%–65% for later use;

[0053] 4. Weigh out 16%–22% friction agent, 0.4%–1.4% thickener, and 0.1%–1% sapote extract by mass and premix them evenly in a stainless steel pot;

[0054] 5. Dissolve 0.01%–0.5% (by mass) of cooling agent and 0.005%–0.1% of rosemary extract in 0.5%–1.3% of fragrance for later use;

[0055] 6. Weigh out a surfactant with a mass fraction of 1.0% to 3.0% for later use;

[0056] 7. Add the sample from step 3 to the ointment preparation pot, add the samples from steps 2 and 1, rinse the container with the remaining deionized water, and stir with a vacuum scraper for 3 minutes;

[0057] 8. Add the sample from step 4, turn on the scraper, stir until there is no obvious dry powder, turn on the vacuum, and start timing for 10 minutes when the vacuum reaches above -0.092MPa;

[0058] 9. Add the samples from steps 5 and 6 and turn on the vacuum scraper to stir for 30 minutes (vacuum not higher than -0.094 MPa);

[0059] 10. Turn off the scraper and stir. Degas under vacuum for more than 10 minutes until there are no obvious bubbles, and obtain a blue auxiliary paste.

[0060] In some embodiments of this application, in step 1 of preparing the blue excipient ointment, 0.1% sodium guaiacol sulfonate is dissolved in 0.5% deionized water by mass.

[0061] In some embodiments of this application, step 2 of the preparation of the blue excipient paste contains 0.1% sodium saccharin, 0.3% sodium benzoate, 0.3% sodium pyrophosphate, 0.2% dipotassium glycyrrhizate, and 0.8% arginine by mass fraction.

[0062] In some embodiments of this application, in step 3 of the preparation of the blue excipient, 50% sorbitol, 3% PEG400, and 8% glycerin by mass fraction are weighed.

[0063] In some embodiments of this application, in step 4 of preparing the blue excipient, 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, and 0.4% safflower extract were weighed.

[0064] In some embodiments of this application, in step 5 of the preparation of the green excipient, 0.1% cooling agent and 0.05% rosemary extract by mass fraction are dissolved in 1% fragrance.

[0065] In some embodiments of this application, step 6 of the preparation of the blue excipient contains 0.5% K12, 0.5% sodium lauroyl sarcosinate, and 0.5% betaine by mass fraction.

[0066] In some embodiments of this application, the preparation method further includes a filling step: the three colored pastes are filled into a toothpaste tube together in a mass ratio of light yellow main paste: green auxiliary paste: blue auxiliary paste = 8~5:1~2.5:1~2.5 to obtain a herbal tricolor strip toothpaste.

[0067] In some embodiments of this application, the mass ratio of pale yellow main paste: green auxiliary paste: blue auxiliary paste in the filling step is 7:1.5:1.5 or 6:2:2.

[0068] This application provides a herbal three-color striped toothpaste and its preparation method. The toothpaste obtains a three-color striped paste without adding exogenous pigments. This toothpaste can achieve a complex effect of freshening breath, inhibiting dental plaque and oral pathogens, anti-inflammatory and swelling reduction, anti-sensitivity, relieving gingivitis, promoting the healing and repair of recurrent oral ulcers, promoting enamel remineralization and porosity repair, with strong synergistic effects, paste stability, and high safety in use. Detailed Implementation

[0069] In this application, the numerical range represented by “~” means the range in which the numerical values ​​recorded before and after “~” are respectively the minimum and maximum values.

[0070] In the numerical ranges described in this application, the upper or lower limit value recorded in a certain numerical range can be replaced with the upper or lower limit value of other numerical ranges described in different stages. In the numerical ranges described in this application, the upper or lower limit value recorded in a certain numerical range can be replaced with the value shown in the embodiments.

[0071] Unless the context clearly indicates otherwise, when used in this specification, the terms "comprising," "including," or "containing" specify the presence of the said element, but do not exclude the presence or addition of one or more other elements.

[0072] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific implementation methods of this application are described in detail below with reference to embodiments and test examples. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0073] Jiubiying, a traditional folk herbal medicine in southern my country, has medicinal records dating back to the "Lingnan Caiyao Lu" (Record of Herbal Drugs from Lingnan) in 1932. It was officially included in the "Chinese Pharmacopoeia (Part I)" in 1977. It has clear effects of clearing heat and detoxifying, cooling the blood and relieving pain, and is widely used in the treatment of external injuries such as bruises and burns. Its extraction process uses the dried bark of *Ilex rotunda* Thunb. (a plant in the Aquifoliaceae family) as raw material. Through ethanol extraction (alcohol extraction), separation and purification, and drying, a characteristic light yellow powder is finally obtained. This extract is rich in triterpenoid saponins, flavonoids, and other active ingredients, making it an important raw material for functional products in the daily chemical and pharmaceutical fields (such as anti-inflammatory toothpaste and topical analgesic ointments).

[0074] This application discloses a three-color striped toothpaste made from traditional Chinese medicine and its preparation method. The purpose is to address the issues of toothpaste having a single functional mechanism, lacking a systematic solution for complex oral pathologies, and the fact that some products claiming to have multiple effects are prone to antagonistic effects between components due to simple mixing of traditional Chinese medicine extracts, resulting in insufficient synergistic efficacy. In addition, the addition of exogenous pigments to toothpaste poses technical problems such as safety risks, stability issues, and the potential for long-term tooth discoloration that affects aesthetics.

[0075] This application provides a method for preparing a herbal tricolor striped toothpaste, including the preparation step of a light yellow main paste:

[0076] 1. Weigh out deionized water (with a suitable amount left to rinse the container), and add in sequence 0.1%–0.22% sweetener, 0%–0.5% preservative, 0.1%–0.5% stabilizer, 0.005%–0.5% dipotassium glycyrrhizate and 0.005%–1% arginine by mass fraction, stir to dissolve evenly and set aside;

[0077] 2. Weigh out a humectant with a mass fraction of 45%–65% for later use;

[0078] 3. Weigh out 16%–22% of abrasive, 0.4%–1.4% of thickener, 0.1%–2% of chitosan quaternary ammonium salt, and 0.1%–1% of strychnine extract by mass and premix them evenly in a stainless steel pot;

[0079] 4. Dissolve 0.01%–0.5% (by mass) of cooling agent and 0.005%–0.1% of rosemary extract in 0.5%–1.3% of fragrance for later use;

[0080] 5. Weigh out a surfactant with a mass fraction of 1.0% to 3.0% for later use;

[0081] 6. Add the sample from step 2 to the ointment preparation pot, then add the sample from step 1 and rinse the container with the remaining deionized water. Stir with a vacuum scraper for 3 minutes.

[0082] 7. Add the sample from step 3, turn on the scraper, stir until there is no obvious dry powder, turn on the vacuum, and start timing for 10 minutes when the vacuum reaches above -0.092MPa;

[0083] 8. Add the samples from steps 4 and 5 and turn on the vacuum scraper to stir for 30 minutes (vacuum not higher than -0.094 MPa);

[0084] 9. Turn off the scraper and stir. Degas under vacuum for more than 10 minutes until there are no obvious bubbles, and you will get a light yellow main paste.

[0085] In some embodiments of this application, the preparation method includes a green excipient preparation step:

[0086] 1. Weigh out 0.5%–1% deionized sodium chlorophyllin and dissolve 0.002%–0.15% sodium copper chlorophyllin.

[0087] 2. Weigh out deionized water (with a suitable amount for rinsing the container), and add 0.1%–0.22% sweetener, 0%–0.5% preservative, 0.1%–0.5% stabilizer, 0.005%–0.5% dipotassium glycyrrhizate, and 0.005%–1.0% arginine by mass fraction, stir and dissolve evenly for later use;

[0088] 3. Weigh out a humectant with a mass fraction of 45%–65% for later use;

[0089] 4. Weigh out 16%–22% friction agent, 0.4%–1.4% thickener, and 0.1%–1% sapote extract by mass and premix them evenly in a stainless steel pot;

[0090] 5. Dissolve 0.01%–0.5% (by mass) of cooling agent and 0.005%–0.1% of rosemary extract in 0.5%–1.3% of fragrance for later use;

[0091] 6. Weigh out a surfactant with a mass fraction of 1.0% to 3.0% for later use;

[0092] 7. Add the sample from step 3 to the ointment preparation pot, add the samples from steps 2 and 1, rinse the container with the remaining deionized water, and stir with a vacuum scraper for 3 minutes;

[0093] 8. Add the sample from step 4, turn on the scraper, stir until there is no obvious dry powder, turn on the vacuum, and start timing for 10 minutes when the vacuum reaches above -0.092MPa;

[0094] 9. Add the samples from steps 5 and 6 and turn on the vacuum scraper to stir for 30 minutes (vacuum not higher than -0.094 MPa);

[0095] 10. Turn off the scraper and stir. Degas under vacuum for more than 10 minutes until there are no obvious bubbles, and obtain the green auxiliary paste.

[0096] In some embodiments of this application, in step 1 of the preparation of the green excipient, 0.5% deionized sodium chlorophyllin is weighed and dissolved to dissolve 0.05% sodium copper chlorophyllin.

[0097] In some embodiments of this application, the preparation method includes a blue excipient preparation step:

[0098] 1. Dissolve 0.1% sodium guaiac sulfonate in 0.5% deionized water;

[0099] 2. Weigh out deionized water (with a suitable amount left to rinse the container), and add in sequence 0.1%–0.22% sweetener, 0%–0.5% preservative, 0.1%–0.5% stabilizer, 0.005%–0.5% dipotassium glycyrrhizate and 0.005%–1.0% arginine by mass fraction, stir and dissolve evenly for later use;

[0100] 3. Weigh out a humectant with a concentration of 45%–65% for later use;

[0101] 4. Weigh out 16%–22% friction agent, 0.4%–1.4% thickener, and 0.1%–1% sapote extract by mass and premix them evenly in a stainless steel pot;

[0102] 5. Dissolve 0.01%–0.5% (by mass) of cooling agent and 0.005%–0.1% of rosemary extract in 0.5%–1.3% of fragrance for later use;

[0103] 6. Weigh out a surfactant with a mass fraction of 1.0% to 3.0% for later use;

[0104] 7. Add the sample from step 3 to the ointment preparation pot, add the samples from steps 2 and 1, rinse the container with the remaining deionized water, and stir with a vacuum scraper for 3 minutes;

[0105] 8. Add the sample from step 4, turn on the scraper, stir until there is no obvious dry powder, turn on the vacuum, and start timing for 10 minutes when the vacuum reaches above -0.092MPa;

[0106] 9. Add the samples from steps 5 and 6 and turn on the vacuum scraper to stir for 30 minutes (vacuum not higher than -0.094 MPa);

[0107] 10. Turn off the scraper and stir. Degas under vacuum for more than 10 minutes until there are no obvious bubbles, and obtain a blue auxiliary paste.

[0108] In some embodiments of this application, the moisturizer is one or more of sorbitol, polyethylene glycol (PEG400), glycerin, and propylene glycol.

[0109] In some embodiments of this application, the friction agent is one or more of hydrated silica, calcium carbonate, calcium phosphate, and aluminum hydroxide.

[0110] In some embodiments of this application, the thickener is one or more of sodium carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC), carrageenan, xanthan gum, polyquaternium-22, guar gum, carbomer, and xanthan gum.

[0111] In some embodiments of this application, the surfactant is one or more of sodium dodecyl sulfate (K12), sodium lauryl sulfate, lauryl glucoside, sodium lauroyl sarcosinate, betaine, sodium lauroyl glutamate, and sodium cocoyl methyl taurate.

[0112] In some embodiments of this application, the sweetener is one or more of sodium saccharin, sucralose, xylitol, erythritol, and steviol glycosides.

[0113] In some embodiments of this application, the preservative is one or more of sodium benzoate, parabens and their salts, and polyols.

[0114] In some embodiments of this application, the stabilizer is one or more of pyrophosphate and phosphate.

[0115] In some embodiments of this application, the light yellow main paste preparation step 1 includes 0.1% sodium saccharin, 0.3% sodium benzoate, 0.3% sodium pyrophosphate, 0.2% dipotassium glycyrrhizate, and 0.8% arginine by mass fraction.

[0116] In some embodiments of this application, in step 2 of preparing the pale yellow main paste, 50% sorbitol, 3% PEG400, and 8% glycerin by mass fraction are weighed.

[0117] In some embodiments of this application, in step 3 of preparing the pale yellow main paste, 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, 0.5% chitosan quaternium salt, and 0.4% scutellaria extract by mass fraction are weighed.

[0118] In some embodiments of this application, in step 4 of preparing the pale yellow main paste, 0.1% cooling agent and 0.05% rosemary extract by mass fraction are dissolved in 1% fragrance.

[0119] In some embodiments of this application, step 5 of the preparation of the pale yellow main paste contains 2.0% K12, 0.5% sodium lauroyl sarcosinate, and 0.5% betaine by mass fraction.

[0120] In some embodiments of this application, step 2 of the preparation of the green excipient contains 0.1% sodium saccharin, 0.3% sodium benzoate, 0.3% sodium pyrophosphate, 0.2% dipotassium glycyrrhizate, and 0.8% arginine by mass fraction.

[0121] In some embodiments of this application, step 3 of the preparation of the green excipient ointment weighs 50% sorbitol, 3% PEG400, and 8% glycerin by mass fraction.

[0122] In some embodiments of this application, step 4 of the preparation of the green excipient ointment weighs 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, and 0.4% safflower extract.

[0123] In some embodiments of this application, in step 5 of the preparation of the green excipient, 0.1% cooling agent and 0.05% rosemary extract by mass fraction are dissolved in 1% fragrance.

[0124] In some embodiments of this application, step 6 of the green excipient preparation step contains 2.0% K12, 0.5% sodium lauroyl sarcosinate, and 0.5% betaine by mass fraction.

[0125] In some embodiments of this application, in step 1 of preparing the blue excipient ointment, 0.1% sodium guaiacol sulfonate is dissolved in 0.5% deionized water by mass.

[0126] In some embodiments of this application, step 2 of the preparation of the blue excipient paste contains 0.1% sodium saccharin, 0.3% sodium benzoate, 0.3% sodium pyrophosphate, 0.2% dipotassium glycyrrhizate, and 0.8% arginine by mass fraction.

[0127] In some embodiments of this application, in step 3 of the preparation of the blue excipient, 50% sorbitol, 3% PEG400, and 8% glycerin by mass fraction are weighed.

[0128] In some embodiments of this application, in step 4 of preparing the blue excipient, 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, and 0.4% safflower extract were weighed.

[0129] In some embodiments of this application, in step 5 of the preparation of the green excipient, 0.1% cooling agent and 0.05% rosemary extract by mass fraction are dissolved in 1% fragrance.

[0130] In some embodiments of this application, step 6 of the preparation of the blue excipient contains 2.0% K12, 0.5% sodium lauroyl sarcosinate, and 0.5% betaine by mass fraction.

[0131] In some embodiments of this application, the preparation method further includes a filling step: the three colored pastes are filled into a toothpaste tube together in a mass ratio of light yellow main paste: green auxiliary paste: blue auxiliary paste = 8~5:1~2.5:1~2.5 to obtain a herbal tricolor strip toothpaste.

[0132] In some embodiments of this application, the mass ratio of pale yellow main paste: green auxiliary paste: blue auxiliary paste in the filling step is 7:1.5:1.5 or 6:2:2.

[0133] Example 1

[0134] Pale yellow base paste process:

[0135] 1. Weigh out deionized water (with a suitable amount left to rinse the container), and add 0.1% sodium saccharin, 0.3% sodium benzoate, 0.3% sodium pyrophosphate, 0.2% dipotassium glycyrrhizate, and 0.8% arginine by mass fraction in sequence. Stir and dissolve evenly for later use.

[0136] 2. Weigh out 50% sorbitol, 3% PEG400, and 8% glycerin by mass fraction and set aside;

[0137] 3. Weigh out 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, 0.5% chitosan quaternium salt, and 0.4% scutellaria extract by mass and premix them evenly in a stainless steel pot;

[0138] 4. Dissolve 0.1% cooling agent and 0.05% rosemary extract in 1% fragrance for later use;

[0139] 5. Weigh out 2.0% K12, 0.5% sodium lauroyl sarcosinate, and 0.5% betaine by mass fraction for later use;

[0140] 6. Add the sample from step 2 to the ointment preparation pot, then add the sample from step 1 and rinse the container with the remaining deionized water. Stir with a vacuum scraper for 3 minutes.

[0141] 7. Add the sample from step 3, turn on the scraper, stir until there is no obvious dry powder, turn on the vacuum, and start timing for 10 minutes when the vacuum reaches above -0.092MPa;

[0142] 8. Add the samples from steps 4 and 5 and turn on the vacuum scraper to stir for 30 minutes (vacuum not higher than -0.094 MPa);

[0143] 9. Turn off the scraper and stir, and degas under vacuum for at least 10 minutes until no obvious bubbles are visible;

[0144] 10. Outgoing material inspection.

[0145] Green auxiliary ointment process:

[0146] 1. Weigh out 0.5% deionized sodium chlorophyllin and dissolve 0.05% sodium copper chlorophyllin.

[0147] 2. Weigh out deionized water (with a suitable amount left to rinse the container), and add 0.1% sodium saccharin, 0.3% sodium benzoate, 0.3% sodium pyrophosphate, 0.2% dipotassium glycyrrhizate, and 0.8% arginine by mass fraction in sequence. Stir and dissolve evenly for later use.

[0148] 3. Weigh out 50% sorbitol, 3% PEG400, and 8% glycerin by mass fraction and set aside;

[0149] 4. Weigh out 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, and 0.4% sapioca extract by mass and premix them evenly in a stainless steel pot;

[0150] 5. Dissolve 0.1% cooling agent and 0.05% rosemary extract in 1% fragrance for later use;

[0151] 6. Weigh out 2.0% K12, 0.5% sodium lauroyl sarcosinate, and 0.5% betaine by mass fraction for later use;

[0152] 7. Add the sample from step 3 to the ointment preparation pot, add the samples from steps 2 and 1, rinse the container with the remaining deionized water, and stir with a vacuum scraper for 3 minutes;

[0153] 8. Add the sample from step 4, turn on the scraper, stir until there is no obvious dry powder, turn on the vacuum, and start timing for 10 minutes when the vacuum reaches above -0.092MPa;

[0154] 9. Add the samples from steps 5 and 6 and turn on the vacuum scraper to stir for 30 minutes (vacuum not higher than -0.094 MPa);

[0155] 10. Turn off the scraper and stir, and degas under vacuum for at least 10 minutes until no obvious bubbles are visible;

[0156] 11. Outgoing material inspection.

[0157] Blue auxiliary ointment process:

[0158] 1. Dissolve 0.1% sodium guaiac sulfonate in 0.5% deionized water;

[0159] 2. Weigh out deionized water (with a suitable amount left to rinse the container), and add 0.1% sodium saccharin, 0.3% sodium benzoate, 0.3% sodium pyrophosphate, 0.2% dipotassium glycyrrhizate, and 0.8% arginine by mass fraction in sequence. Stir and dissolve evenly for later use.

[0160] 3. Weigh out 50% sorbitol, 3% PEG400, and 8% glycerin by mass fraction and set aside;

[0161] 4. Weigh out 20% hydrated silica, 0.6% polyquaternium-22, 0.3% xanthan gum, 0.3% guar gum, and 0.4% scutellaria extract by mass and premix them evenly in a stainless steel pot;

[0162] 5. Dissolve 0.1% cooling agent and 0.05% rosemary extract in 1% fragrance for later use;

[0163] 6. Weigh out 2.0% K12, 0.5% sodium lauroyl sarcosinate, and 0.5% betaine by mass fraction for later use;

[0164] 7. Add the sample from step 3 to the ointment preparation pot, add the samples from steps 2 and 1, rinse the container with the remaining deionized water, and stir with a vacuum scraper for 3 minutes;

[0165] 8. Add the sample from step 4, turn on the scraper, stir until there is no obvious dry powder, turn on the vacuum, and start timing for 10 minutes when the vacuum reaches above -0.092MPa;

[0166] 9. Add the samples from steps 5 and 6 and turn on the vacuum scraper to stir for 30 minutes (vacuum not higher than -0.094 MPa);

[0167] 10. Turn off the scraper and stir, and degas under vacuum for at least 10 minutes until no obvious bubbles are visible;

[0168] 11. Outgoing material inspection.

[0169] Filling: The three colored pastes are filled into toothpaste tubes in a mass ratio of light yellow main paste: green auxiliary paste: blue auxiliary paste = 11:4:4 to obtain herbal three-color strip toothpaste.

[0170] Test Example 1: Quality Assessment of Toothpaste

[0171] The consistency, specific gravity, foam volume, and pH value of the pale yellow main paste, green auxiliary paste, and blue auxiliary paste in the herbal three-color strip toothpaste of this application were tested to determine whether each indicator met the national standard and industry standard testing range. Consistency: 9-15mm (immediately), 12-18mm (the next day); Specific gravity: 1.2-1.5; Foam volume: 100-150mm; pH: 5.5-10.0.

[0172] 1. Experimental Instruments and Equipment

[0173] Consistency meter: Used to measure the consistency of toothpaste, with an accuracy of 1 mm.

[0174] Specific gravity bottle: with a capacity of 50 mL, used to determine the specific gravity of toothpaste.

[0175] Foam quantity measuring device: including a 250mL stoppered graduated cylinder (accurate to 1mm) and a constant temperature water bath (temperature controlled at 25±2℃).

[0176] pH meter: with an accuracy of 0.01, used to measure the pH value of toothpaste.

[0177] Electronic balance: with an accuracy of 0.0001g, used for weighing specific gravity bottles and sample mass.

[0178] 2. Experimental steps (1) Consistency detection

[0179] Instant consistency measurement: Place the consistency meter on a level surface beforehand and calibrate the instrument to ensure the pointer points to zero. Take an appropriate amount (approximately 50g) of the toothpaste sample to be tested (light yellow main paste, green auxiliary paste, and blue auxiliary paste are tested separately) and place it in a beaker. Stir well with a glass rod, avoiding the introduction of air bubbles. Carefully fill the sample container of the consistency meter with the stirred toothpaste. After filling, scrape off the excess toothpaste on the surface with a glass rod to make the sample surface smooth. Quickly and gently place the plunger of the consistency meter on the sample surface, ensuring that the plunger is in complete contact with the sample surface and perpendicular to it. Release the plunger and start the stopwatch simultaneously. After 30 seconds, read the distance the plunger sinks, which is the instant consistency in mm. Repeat the measurement 3 times for each sample and take the average value as the instant consistency of that sample.

[0180] Second day consistency measurement: Following the method described above for instant consistency measurement, place the prepared toothpaste sample (filling the consistency meter sample container and smoothing the surface) in a laboratory environment (temperature 25±2℃, relative humidity 50±5%) and let it stand for 24 hours. After 24 hours, gently place the plunger of the consistency meter on the sample surface again, ensuring that the plunger is in complete contact with the sample surface and perpendicular to it. Release the plunger, and after timing for 30 seconds, read the distance the plunger sinks; this is the consistency for the second day. Repeat the measurement 3 times for each sample and take the average value.

[0181] (2) Specific gravity test

[0182] Calibration of a specific gravity bottle: Weigh the empty specific gravity bottle using an electronic balance and record the mass as m0, accurate to 0.0001g; fill the specific gravity bottle with distilled water (the temperature of the distilled water is controlled at 25±2℃), stopper the bottle, and allow excess water to overflow from the capillary tube of the stopper. Wipe the water on the outside of the specific gravity bottle with filter paper; weigh the specific gravity bottle filled with distilled water again and record the mass as m1, accurate to 0.0001g; calculate the volume of the specific gravity bottle V = (m1-m0) / ρ based on the density ρ of distilled water at 25℃ (0.9970g / mL).

[0183] Sample specific gravity measurement: Pour out the distilled water from the specific gravity bottle, rinse the specific gravity bottle 2-3 times with the toothpaste samples to be tested (light yellow main paste, green auxiliary paste, and blue auxiliary paste respectively), then fill the specific gravity bottle with toothpaste samples, press the sample with a glass rod to remove air bubbles, and make the sample surface flush with the bottle mouth; stopper the bottle, wipe the outside of the specific gravity bottle dry with filter paper, weigh the specific gravity bottle filled with toothpaste samples, record it as m2, accurate to 0.0001g.

[0184] Calculate the specific gravity of the toothpaste sample: Specific gravity = (m2-m0) / V. Each sample is measured three times, and the average value is taken as the specific gravity of the sample.

[0185] (3) Foam quantity detection

[0186] Preparation: Adjust the constant temperature water bath to 25±2℃, and preheat a 250mL stoppered graduated cylinder in the water bath for 10 minutes; weigh 2.0g (accurate to 0.01g) of the toothpaste sample to be tested (light yellow main paste, green auxiliary paste, and blue auxiliary paste are tested separately), put them into the preheated stoppered graduated cylinder, and add 80mL of distilled water at a temperature of 25±2℃.

[0187] Foam generation by oscillation: Tighten the stopper of the graduated cylinder and oscillate it up and down 30 times at a speed of 2 times per second, with an oscillation amplitude of 30-40 cm. After oscillation, immediately place the graduated cylinder vertically in a constant temperature water bath and let it stand for 1 minute. Then read the height of the foam in mm. Repeat the measurement 3 times for each sample and take the average value as the amount of foam for that sample.

[0188] (4) pH value detection

[0189] pH meter calibration: Turn on the pH meter and preheat for 30 minutes; perform two-point calibration of the pH meter using standard buffer solutions (phosphate buffer pH=6.86 and borax buffer pH=9.18, at 25℃) to ensure that the calibration error is within ±0.01.

[0190] Sample Measurement: Take an appropriate amount of toothpaste sample to be tested (light yellow main paste, green auxiliary paste, and blue auxiliary paste are tested separately), put them in a beaker, add a small amount of distilled water (about 50 mL), stir evenly with a glass rod to make a toothpaste solution; rinse the electrode of the pH meter with distilled water, dry the surface of the electrode with filter paper, then insert the electrode into the toothpaste solution, and after the reading stabilizes, read the pH value, accurate to 0.01. Repeat the measurement 3 times for each sample, and take the average value as the pH value of the sample.

[0191] 3. Data Recording and Processing

[0192] The measurement data of each sample of the herbal tricolor strip toothpaste under various test indicators were recorded according to the experimental design, as shown in Table 1 below.

[0193] Table 1. Detection Indicators and Results of Herbal Three-Color Striped Toothpaste

[0194] Product Name Instant consistency (mm) The consistency on the second day was mm proportion Foam volume (mm) pH pale yellow base cream 15 18 1.327 130 7.80 Green auxiliary paste 15 18 1.317 140 7.72 Blue auxiliary paste 12 15 1.323 140 7.70

[0195] The consistency, specific gravity, foam volume, and pH value of the light yellow main paste, green auxiliary paste, and blue auxiliary paste in the three-color strip toothpaste all meet the national standards and industry standard testing ranges. Among them, the pH value and foam volume are excellent, and the consistency and specific gravity are within a reasonable range.

[0196] Test Example 2: Toothpaste Antibacterial Experiment Protocol

[0197] The antibacterial activity of the three-color toothpaste, single-color toothpaste, and blank toothpaste of this application was evaluated using the in vitro agar diffusion method (KB method) with common oral pathogens (Streptococcus mutans, Porphyromonas gingivalis, and Candida albicans) as targets.

[0198] I. Experimental Materials and Equipment

[0199] (I) Test Samples

[0200] Three-color striped toothpaste (mixed paste, designated as group S);

[0201] Pale yellow base cream (labeled Group A);

[0202] Green auxiliary ointment (referred to as Group B);

[0203] Blue auxiliary ointment (labeled Group C);

[0204] Blank control: Blank ointment (a solution of blank ointment without dipotassium glycyrrhizate, arginine, chitosan quaternary ammonium salt, scutellaria barbata extract, sodium copper chlorophyllin, sodium guaiacol sulfonate and rosemary extract, designated as group D).

[0205] (II) Target Strains

[0206] Streptococcus mutans (ATCC 25175, a cariogenic bacterium);

[0207] Porphyromonas gingivalis (ATCC 33277, a periodontitis pathogen);

[0208] Candida albicans (ATCC 10231, oral fungus).

[0209] (III) Culture Medium

[0210] Brain heart infusion agar (BHIAgar, used for bacterial culture);

[0211] Sabouraud agar (SDAAgar, used for fungal culture).

[0212] II. Testing Methods and Procedures

[0213] (I) Sample Pretreatment

[0214] Dissolve the toothpaste by taking 1.0g of each toothpaste sample (S / A / B / C group), adding 9mL of sterile physiological saline, vortexing to mix, centrifuging at 3000rpm for 10 minutes, and taking the supernatant as the test solution (if the toothpaste contains insoluble particles, it needs to be filtered through a 0.45μm sterile filter membrane).

[0215] Negative control: Take an equal amount of blank paste (Group D) and prepare a matrix solution according to the above method.

[0216] Concentration calibration ensures that the concentration of the paste in the test solution is 10% (w / v), that is, 1g of paste corresponds to 10mL of solution (if the volume changes after centrifugation, physiological saline needs to be added to adjust).

[0217] (II) Preparation of bacterial suspension

[0218] Bacteria (Streptococcus mutans, Porphyromonas gingivalis): After resuscitation, the strains were inoculated into BHI liquid medium and incubated at 37°C for 24 hours. The bacterial concentration was adjusted to 1×10⁻⁶ with 0.85% physiological saline. 8 CFU / mL.

[0219] Fungus (Candida albicans): Inoculated onto SDA liquid medium and incubated at 30°C for 48 hours. The bacterial concentration was adjusted to 1×10⁻⁶ with 0.85% physiological saline. 6 CFU / mL.

[0220] (III) Preparation of Agar Plates

[0221] Under aseptic conditions, pour the melted BHI agar (bacteria) or SDA agar (fungi) into a sterile petri dish (90 mm in diameter), about 4 mm thick (about 15-20 mL per dish), and let it solidify before use.

[0222] (iv) Determination of inhibition zone (Oxford cup method)

[0223] Bacterial film coating: Use a sterile cotton swab to dip into the bacterial suspension and coat it evenly on the agar surface 3 times, rotating 90° each time to ensure even distribution of the bacterial solution. Let it stand for 5 minutes to allow the surface to dry.

[0224] Sample addition procedure: Place the Oxford cup in the center of the plate with sterile forceps and gently press it to ensure it adheres tightly to the agar; add 20 μL of test solution (groups S / A / B / C / D) to each Oxford cup, taking care to avoid air bubbles and overflow; set up 3 parallel plates for each group of strains, for a total of 18 plates.

[0225] Culture and observation: Bacteria were incubated upside down at 37°C for 48 hours (Streptococcus mutans requires an anaerobic environment, and Porphyromonas gingivalis requires an anaerobic incubator); fungi were incubated upside down at 30°C for 72 hours, and the formation of inhibition zones was observed.

[0226] Data recording and processing: Measure the vertical distance from the center of the Oxford cup to the edge of the inhibition zone using calipers (accurate to 0.1 mm). The diameter of the inhibition zone is twice the measured value (including the Oxford cup diameter of 6 mm). If bacteria grow in some areas close to the Oxford cup, use the smallest diameter for completely sterile growth. If the edge is blurred, calculate based on the edge of the visible transparent area. Data recording and processing are shown in Table 2 below.

[0227] Table 2. Diameter of the inhibition zone

[0228] Test group Streptococcus mutans (mm) Porphyromonas gingivalis (mm) Candida albicans (mm) Group D 6.0±0.0 6.0±0.0 6.0±0.0 Group S 27.5±1.2 26.8±1.0 23.3±0.9 Group A 9.2±0.8 8.5±0.7 6.8±0.6 Group B 10.5±1.0 10.8±0.9 9.2±0.8 Group C 9.8±0.9 8.5±0.6 6.9±0.5

[0229] The blank control group D was 6 mm, which is the diameter of the Oxford cup, and had no antibacterial activity. Therefore, the effective antibacterial activity should be greater than the diameter of the inhibition zone (6 mm, i.e., greater than the diameter of the Oxford cup). The larger the diameter of the inhibition zone, the stronger the antibacterial activity. In the single-color toothpaste, the inhibition zone diameters of Group A (light yellow main paste), Group B (green auxiliary paste), and Group C (blue auxiliary paste) against the three pathogenic bacteria (Streptococcus mutans, Porphyromonas gingivalis, and Candida albicans) were almost identical, indicating that they all had antibacterial activity. This is related to the presence of natural antibacterial ingredients in the toothpaste, such as dipotassium glycyrrhizate, arginine, scutellaria barbata extract, rosemary extract, sodium copper chlorophyllin, and sodium guaiacol sulfonate. The inhibition zone diameter of Group S (three-color striped toothpaste) against the three pathogenic bacteria (Streptococcus mutans, Porphyromonas gingivalis, and Candida albicans) was significantly larger than that of each single-color group (Group A / Group B / Group C). The multi-component compound produced a synergistic antibacterial effect, indicating that the herbal three-color striped toothpaste of this application has a superior inhibitory effect on common oral pathogens.

[0230] Test Example 3: Anti-inflammatory and Swelling Reduction Test

[0231] The effects of the three-color toothpaste, single-color toothpaste, and blank toothpaste of this application on anti-inflammatory and swelling-reducing effects in mice were tested.

[0232] I. Sample to be tested (Example 1)

[0233] Three-color striped toothpaste (mixed paste, designated as group S);

[0234] Pale yellow base cream (labeled Group A);

[0235] Green auxiliary ointment (referred to as Group B);

[0236] Blue auxiliary ointment (labeled Group C);

[0237] Blank control: Blank ointment (a solution of blank ointment without dipotassium glycyrrhizate, arginine, chitosan quaternary ammonium salt, scutellaria barbata extract, sodium copper chlorophyllin, sodium guaiacol sulfonate and rosemary extract, designated as group D).

[0238] II. Testing Methods and Procedures

[0239] Dosage: The dosage for both the experimental groups (S, A, B, and C) and the blank control group (D) was 5 g / kg, once a day, administered via topical application.

[0240] Mouse ear swelling experiment: SPF grade KM mice, 18-22g, male, were randomly divided into experimental groups (S group, A group, B group, C group) and blank control group (D group), a total of 5 groups, with 10 mice in each group.

[0241] Preparation before inflammation: Before inducing inflammation, the mouse abdomen was shaved (approximately 3.0cm × 3.0cm) to avoid skin damage.

[0242] Modeling and drug administration: Apply 1 mL or 1 g (approximately 2.5 cm × 2.5 cm) of the drug to the abdomen for 7 days, or apply the drug via a swab for 7 days. 0.5 h after the last administration, apply 0.02 ml of 100% xylene to both sides of the left ear of each mouse to induce inflammation, with the right ear serving as a control. Two hours after inducing inflammation, sacrifice the animals, cut off both ears along the auricle baseline, and punch out circular pieces from the same location on both ears using a 9 mm diameter punch. Weigh the pieces using an electronic analytical balance (accurate to 0.1 g) and calculate the degree of swelling and the swelling inhibition rate.

[0243] III. Test Results

[0244] The swelling degree was calculated according to the formula: Swelling degree = Weight of left ear piece - Weight of right ear piece, Swelling inhibition rate % = (Swelling degree of blank control group - Swelling degree of experimental group) / Swelling degree of blank control group × 100%. The results of the mouse ear swelling degree and swelling inhibition rate test are shown in Table 3 below.

[0245] Table 3. Xylene-induced ear swelling degree and swelling inhibition rate

[0246]

[0247]

[0248] Note: Compared with the blank control group, *P<0.05

[0249] Compared to group D, group S (three-color striped toothpaste) achieved a swelling inhibition rate of 90.6%, significantly higher than groups A / B / C (single-color toothpaste, with swelling inhibition rates all less than 25%). This indicates that the mixed components have a synergistic anti-inflammatory effect. Dipotassium glycyrrhizate, arginine, chitosan quaternary ammonium salt, rosemary extract, and safflower extract in the toothpaste are the main anti-inflammatory components. The anti-inflammatory and swelling-reducing effects of the compounded three-color striped toothpaste are superior to those of single components and most controls, with a significant enhancement of approximately 3.6 times. This confirms the synergistic advantage of the multi-component compounded anti-inflammatory components, indicating that the traditional Chinese medicine three-color striped toothpaste of this application has a superior anti-inflammatory and swelling-reducing effect on common oral pathogens and has the potential to be developed into a functional oral care product.

[0250] Test Example 4: Enamel Remineralization and Pore Repair Test

[0251] The effects of the three-color toothpaste, single-color toothpaste, and blank toothpaste of this application on enamel remineralization and porosity repair were tested.

[0252] I. Experimental Materials

[0253] Blank toothpaste: Basic formula toothpaste (without chitosan quaternary ammonium salt);

[0254] Three-color striped toothpaste (mixed paste, designated as group S);

[0255] Pale yellow base cream (labeled Group A);

[0256] Green auxiliary ointment (referred to as Group B);

[0257] Blue auxiliary ointment (labeled Group C);

[0258] Blank control: Blank ointment (a solution of blank ointment without dipotassium glycyrrhizate, arginine, chitosan quaternary ammonium salt, scutellaria barbata extract, sodium copper chlorophyllin, sodium guaiacol sulfonate and rosemary extract, designated as group D).

[0259] II. Experimental Procedure

[0260] Sample preparation: 30 extracted human molars were prepared into 1 mm thick enamel slides, which were ultrasonically cleaned to obtain undemineralized enamel slides for later use; the intensity (relative value) of the characteristic peak of hydroxyapatite on the undemineralized enamel slides was 1.00±0.05; SEM observation showed that the surface was smooth and without pores.

[0261] Demineralization treatment: Undemineralized enamel tablets were divided into 5 groups of 5 tablets each and immersed in a demineralization solution of 0.1 mol / L lactic acid and 0.01 mol / L calcium chloride (pH 4.5) and shaken at 37°C for 6 hours.

[0262] Group intervention: The demineralized enamel slices of each group were placed in centrifuge tubes containing a mixture of the corresponding toothpaste (2g) and artificial saliva (20mL) and cultured at 37℃ in a constant temperature shaking incubator for 7 days, with the medium changed every 2 days.

[0263] Detection and analysis: The intensity of characteristic peaks of hydroxyapatite was determined by FT-IR spectroscopy to quantify the mineral content; the porosity repair of the enamel surface was observed by SEM microscopy. The experimental data are shown in Table 4 below.

[0264] Table 4. Intensities of characteristic peaks of hydroxyapatite in tooth enamel sections and SEM observation results.

[0265]

[0266] The experimental results showed that compared with group D (blank toothpaste), groups S and A containing chitosan quaternary ammonium salt showed significant improvements in the intensity of the characteristic peak of hydroxyapatite in tooth enamel and the repair of enamel surface porosity; while compared with group D, groups B and C showed no significant changes in the intensity of the characteristic peak of hydroxyapatite in tooth enamel and the repair of enamel surface porosity.

[0267] Further analysis revealed that group S showed at least twice the enhancement effect of group A in the intensity of the characteristic peak of hydroxyapatite in tooth enamel. Combined with SEM observation results, the porosity of the enamel surface in group S was almost completely repaired, while group A still had many depressions. The repair status of groups B and C was not significantly different from that of group D. Therefore, chitosan quaternary ammonium salt can effectively promote enamel remineralization and repair of enamel surface porosity. Compared with single-color toothpaste (groups A, B, and C), the herbal tricolor striped toothpaste of this application (group S) is significantly superior in promoting enamel remineralization and porosity repair. This indicates a synergistic effect between the combination of multiple ingredients and chitosan quaternary ammonium salt, which can significantly enhance the effect of promoting enamel remineralization and porosity repair, confirming the effectiveness and advantages of the herbal tricolor striped toothpaste of this application in this regard.

[0268] This application provides a herbal three-color striped toothpaste and its preparation method. The toothpaste obtains a three-color striped paste without adding exogenous pigments. This toothpaste can achieve a complex effect of freshening breath, inhibiting dental plaque and oral pathogens, anti-inflammatory and swelling reduction, anti-sensitivity, relieving gingivitis, promoting the healing and repair of recurrent oral ulcers, promoting enamel remineralization and porosity repair, with strong synergistic effects, paste stability, and high safety in use.

[0269] The above description of the embodiments disclosed in this application enables those skilled in the art to make or use this application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of this application. Therefore, this application is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A herbal three-color striped toothpaste, characterized in that, The product includes a pale yellow paste, a green paste, and a blue paste. The pale yellow paste comprises 0.1% to 2% by mass of chitosan quaternary ammonium salt and 0.1% to 1% by mass of *Smilax china* extract. The green paste comprises 0.002% to 0.15% by mass of sodium copper chlorophyllin and 0.1% to 1% by mass of *Smilax china* extract. The blue paste comprises 0.03% to 0.15% by mass of sodium guaiacol and 0.1% to 1% by mass of *Smilax china* extract.

2. The herbal tricolor striped toothpaste according to claim 1, characterized in that, The pale yellow paste, the green paste, and the blue paste each contain 0.001% to 0.05% rosemary extract, 0.005% to 0.5% dipotassium glycyrrhizate, and 0.005% to 1.0% arginine by mass fraction.

3. A toothpaste containing the herbal tricolor stripe as described in claim 2, characterized in that, The pale yellow paste, the green paste, and the blue paste each comprise the following components by mass fraction: 45%–65% humectant, 16%–22% abrasive, 0.4%–1.4% thickener, 1.0%–2.5% surfactant, 0.5%–1.3% fragrance, 0.1%–0.22% sweetener, 0%–0.5% preservative, 0.1%–0.5% stabilizer, and the balance being water.

4. The herbal tricolor striped toothpaste according to claim 3, characterized in that, The moisturizer is one or more of sorbitol, polyethylene glycol, glycerin, and propylene glycol.

5. The herbal tricolor striped toothpaste according to claim 3, characterized in that, The friction agent is one or more of hydrated silica, calcium carbonate, calcium phosphate, and aluminum hydroxide.

6. The herbal tricolor striped toothpaste according to claim 3, characterized in that, The thickener is one or more of the following: sodium carboxymethyl cellulose, hydroxyethyl cellulose, polyquaternium-22, carrageenan, xanthan gum, guar gum, and carbomer.

7. The herbal tricolor striped toothpaste according to claim 3, characterized in that, The surfactant is one or more of sodium dodecyl sulfate, sodium lauryl sulfate, lauryl glucoside, sodium lauroyl sarcosinate, betaine, sodium lauroyl glutamate, and sodium cocoyl methyl taurate.

8. The herbal tricolor striped toothpaste according to claim 3, characterized in that, The sweetener is one or more of sodium saccharin, sucralose, xylitol, erythritol, and steviol glycosides.

9. The herbal tricolor striped toothpaste according to claim 3, characterized in that, The preservative is one or more of sodium benzoate, parabens and their salts, and polyols.

10. The herbal tricolor striped toothpaste according to claim 3, characterized in that, The stabilizer is one or more of pyrophosphate and phosphate.

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