Porcine pseudorabies GE purification scheme

Abstract

The invention discloses a porcine pseudorabies GE purification scheme, and the purification method comprises the following steps: S1, pig farm environment background evaluation and odor purification device installation; s2, evaluating the health status of the swinery and optimizing an immune program; s3, epidemic disease purification and odor cooperative control; s4, environment-epidemic disease linkage monitoring and early warning are carried out; and S5, maintaining the purification effect. The environment stress is reduced through the odor purification device, the immunity of pigs is enhanced, and the propagation opportunity of PRV is reduced; biodegradation and photocatalysis technologies are combined, so that a remarkable inactivation effect on PRV aerosol is achieved; establishing a first machine learning-based environment parameter and PRV propagation risk prediction model to realize early warning; the use amount of disinfectants is reduced by 40%, the use amount of antibiotics is reduced, and green breeding is achieved; the PRV purification period can be shortened, the maintenance cost is reduced, and meanwhile, the working environment of a pig farm is remarkably improved.

Description

Technical Field

[0001] This invention relates to the field of pig farming technology, specifically to a GE purification solution for swine pseudorabies. Background Technology

[0002] Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV), resulting in significant economic losses to the global pig farming industry. Current eradication methods primarily focus on biosecurity measures such as vaccination and culling. However, these methods have the following shortcomings:

[0003] 1. The impact of environmental factors on the spread of disease in pig farms was not considered. In particular, harmful gases such as ammonia and hydrogen sulfide in the odor can damage the respiratory mucosa of pigs and increase the risk of PRV infection.

[0004] 2. Traditional disinfection methods have limited effectiveness in removing airborne virus particles;

[0005] 3. The disposal of dead pigs generated during the purification process may cause secondary pollution, and the synergistic effect of environmental control and disease purification is not involved. Summary of the Invention

[0006] The problem this invention aims to solve is to provide a GE (Genomic Odor Control) solution for porcine pseudorabies (PRV), which reduces environmental stress, enhances pig immunity, and reduces the chance of PRV transmission through an odor purification device; combines biodegradation and photocatalysis technologies to achieve significant inactivation of PRV aerosols; establishes the first machine learning-based environmental parameter and PRV transmission risk prediction model to achieve early warning; reduces disinfectant usage by 40% and antibiotic use, achieving green farming; shortens the PRV purification cycle, reduces maintenance costs, and significantly improves the working environment of pig farms.

[0007] The technical solution provided by this invention to solve the above problems is: a method for purging porcine pseudorabies GE, the purification method comprising the following steps,

[0008] S1. Baseline environmental assessment and odor purification device installation in pig farms: Test the air quality in pig houses and measure the concentrations of ammonia, hydrogen sulfide, and carbon dioxide; at the same time, install odor purification devices in the ventilation system of pig houses; establish a real-time environmental parameter monitoring system to maintain ammonia concentration <10ppm and hydrogen sulfide concentration <5ppm.

[0009] S2. Swine herd health status assessment and immunization program optimization: Multiplex quantitative PCR was used to detect PRV wild-type virus, vaccine strain, and porcine circovirus; ELISA was used to detect GB and GE antibodies; differentiated immunization programs were developed based on the test results; and an immunization efficacy evaluation system was established.

[0010] S3. Disease purification and odor control: The isolation pens for positive pigs are equipped with independent odor purification units and ozone generation modules; at the same time, a tiered elimination strategy is adopted; the disinfection of the pigsty adopts an alternating scheme of "dry fog hydrogen peroxide - micro-electrolysis of water", and the odor purification device is activated simultaneously during disinfection;

[0011] S4. Environmental-Disease Linkage Monitoring and Early Warning: Establish a correlation model between environmental parameters and disease occurrence; activate enhanced monitoring procedures when ammonia concentration is >15ppm for 3 consecutive days; develop a mobile APP to display environmental data and disease risk levels in real time;

[0012] S5. Purification effect maintenance: Conduct environmental microbial testing monthly, focusing on monitoring PRV load in aerosols; replace the microbial agent in the odor purification device quarterly; newly introduced breeding pigs must undergo a three-stage procedure of "isolation-testing-odor adaptation".

[0013] Preferably, the differential immunization procedure in S2 includes,

[0014] GE antibody-negative pig herds: Primary immunization with gene-deleted vaccines;

[0015] For GE antibody-positive pigs: strengthen immunization and use interferon inducers.

[0016] Preferably, the immune effect evaluation system in S2 uses a neutralizing antibody titer ≥1:16 as the protection standard.

[0017] Preferably, the tiered elimination strategy in S3 includes,

[0018] Observation of markers in pigs that tested positive for the first time;

[0019] Pigs that test positive a second time will be immediately culled and transported to the biosafety processing area via a dedicated channel.

[0020] Preferably, the "dry fog hydrogen peroxide-micro-electrolysis water" disinfection scheme in S3 is as follows:

[0021] Use hydrogen peroxide dry fog disinfection once a week, with an action time of 60 minutes;

[0022] Disinfect by spraying micro-electrolyzed water every two weeks.

[0023] Preferably, the hydrogen peroxide is 5% hydrogen peroxide.

[0024] Preferably, the pH value of the micro-electrolyzed water is 2.5.

[0025] Preferably, the amount of water used in the micro-electrolysis is 10 mL / m 3 .

[0026] Preferably, the method for establishing the correlation model between environmental parameters and disease occurrence includes,

[0027] Collect historical environmental data and concurrent disease testing results;

[0028] Key environmental factors were analyzed using the random forest algorithm;

[0029] Determine the dose-response relationship between ammonia concentration, relative humidity, and PRV positivity.

[0030] Preferably, the odor purification device in S1 includes a shell, a microbial treatment component, and a nutrient solution delivery component, both of which are disposed inside the shell; the microbial treatment component includes a treatment box and several microbial treatment modules, which are installed vertically at intervals within the treatment box and are interconnected; the nutrient solution delivery component is used to deliver nutrient solution to the several microbial treatment modules.

[0031] Compared with existing technologies, the advantages of this invention are: it reduces environmental stress, enhances pig immunity, and reduces the chance of PRV transmission through an odor purification device; it combines biodegradation and photocatalysis technologies to have a significant inactivation effect on PRV aerosols; it establishes the first machine learning-based environmental parameter and PRV transmission risk prediction model to achieve early warning; it reduces disinfectant usage by 40%, reduces antibiotic use, and achieves green farming; it can shorten the PRV purification cycle, reduce maintenance costs, and significantly improve the working environment of pig farms. Attached Figure Description

[0032] The accompanying drawings, which are provided to further illustrate the invention and constitute a part of this invention, are illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention.

[0033] Figure 1 This is a flowchart of the purification solution of the present invention;

[0034] Figure 2 This is a three-dimensional structural schematic diagram of the present invention;

[0035] Figure 3 This is a cross-sectional view of the present invention;

[0036] Figure 4 yes Figure 3 Enlarged view of point A in the middle;

[0037] Figure 5 yes Figure 4 Enlarged view of point B in the middle;

[0038] Figure 6 yes Figure 4 Enlarged view of point C in the middle;

[0039] Figure 7This is a top view of the pre-filter component of the present invention;

[0040] Figure 8 This is a top view of the pre-filter assembly of the present invention after the filter module has been removed;

[0041] Figure 9 This is a cross-sectional view of the pre-filter assembly of the present invention. Figure 1 ;

[0042] Figure 10 yes Figure 9 Enlarged view of point D in the middle;

[0043] Figure 11 This is a cross-sectional view of the pre-filter assembly of the present invention. Figure 2 ;

[0044] Figure 12 This is a cross-sectional view of the positioning component of the present invention in conjunction with the mounting frame;

[0045] Figure 13 This is a cross-sectional view of the pre-filter assembly of the present invention. Figure 3 ;

[0046] Figure 14 yes Figure 13 Enlarged diagram of point E in the middle.

[0047] Attached diagram labels: 1. Outer shell, 2. Exhaust pipe, 3. Filter box, 4. Storage box, 5. Transfer pump, 6. Transfer pipe, 7. Spray plate, 8. Spray head, 9. Packing material, 10. Support plate, 11. Processing box, 12. Filter chamber, 13. Mating plate, 14. Rotating plate, 15. Filter screen, 16. Pressure plate, 17. Limiting plate, 18. Spring 2, 19. Drive rod, 20. Mounting hole 3, 21. Positioning rod 2, 22. Transmission gear 2, 23. Gear 1. 24. Transmission gear 1; 25. Mounting hole 2; 26. Mounting frame; 27. Slot 2; 28. Mounting hole 4; 29. ​​Transmission rod; 30. Gear 5; 31. Spring 3; 32. Positioning rod 3; 33. Limiting groove; 34. Indicator plate; 35. Rotating shaft; 36. Mounting groove; 37. Spring 1; 38. Positioning rod 1; 39. Divider plate; 40. Slot 1; 41. Transmission gear 3; 42. Gear 2; 43. Gear 4; 44. Gear 3. Detailed Implementation

[0048] The following will describe in detail the implementation of the present invention with reference to the accompanying drawings and embodiments, so that the process of how the present invention uses technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0049] In the description of this invention, it should be noted that the directional terms such as "center", "lateral", "longitudinal", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", and "counterclockwise" indicate the orientation and positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. They should not be construed as limiting the specific protection scope of this invention.

[0050] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features. Thus, the use of "first" and "second" to define a feature may explicitly or implicitly include one or more of that feature, and in the description of this invention, "a number" means two or more, unless otherwise explicitly specified.

[0051] In this invention, unless otherwise explicitly specified and limited, the terms "assembly," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can also refer to a mechanical connection; they can refer to a direct connection or a connection through an intermediate medium; or they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0052] It should be understood that, when used in this specification and the appended claims, the terms "comprising" and "including" indicate the presence of the described features, integrals, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integrals, steps, operations, elements, components and / or collections thereof.

[0053] It should also be understood that the terminology used in this specification of embodiments of the invention is for the purpose of describing particular embodiments only and is not intended to limit the embodiments of the invention. As used in this specification of embodiments of the invention and the appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms unless the context clearly indicates otherwise.

[0054] Example 1

[0055] like Figure 1 As shown in the figure, this embodiment discloses a method for purging porcine pseudorabies GE, the purification method including the following steps:

[0056] S1. Baseline environmental assessment and odor purification device installation in pig farms: Test the air quality in pig houses and measure the concentrations of ammonia, hydrogen sulfide, and carbon dioxide; at the same time, install odor purification devices in the ventilation system of pig houses; establish a real-time environmental parameter monitoring system to maintain ammonia concentration <10ppm and hydrogen sulfide concentration <5ppm.

[0057] S2. Assessment of pig herd health status and optimization of immunization program: Multiplex quantitative PCR was used to detect PRV wild-type virus, vaccine strain and porcine circovirus; ELISA was used to detect GB and GE antibodies; a differentiated immunization program was developed based on the test results; an immunization efficacy evaluation system was established, with a neutralizing antibody titer ≥1:16 as the protection standard;

[0058] S3. Disease purification and odor control: The isolation pens for positive pigs are equipped with independent odor purification units and ozone generation modules; at the same time, a tiered elimination strategy is adopted; the disinfection of the pigsty adopts an alternating scheme of "dry fog hydrogen peroxide - micro-electrolysis of water", and the odor purification device is activated simultaneously during disinfection;

[0059] S4. Environmental-Disease Linkage Monitoring and Early Warning: Establish a correlation model between environmental parameters and disease occurrence; activate enhanced monitoring procedures when ammonia concentration is >15ppm for 3 consecutive days; develop a mobile APP to display environmental data and disease risk levels in real time;

[0060] S5. Purification effect maintenance: Conduct environmental microbial testing monthly, focusing on monitoring PRV load in aerosols; replace the microbial agent in the odor purification device quarterly; newly introduced breeding pigs must undergo a three-stage procedure of "isolation-testing-odor adaptation".

[0061] The differential immunization procedure in S2 includes,

[0062] GE antibody-negative pig herds: Primary immunization with gene-deleted vaccines;

[0063] For GE antibody-positive pigs: strengthen immunization and use interferon inducers.

[0064] The tiered elimination strategy in S3 includes,

[0065] Observation of markers in pigs that tested positive for the first time;

[0066] Pigs that test positive a second time will be immediately culled and transported to the biosafety processing area via a dedicated channel.

[0067] The "dry fog hydrogen peroxide-micro-electrolysis water" disinfection scheme in S3 is as follows:

[0068] Disinfect with 5% hydrogen peroxide dry fog once a week, with an action time of 60 minutes.

[0069] Disinfect by spraying with pH 2.5 micro-electrolyzed water every two weeks, at a dosage of 10 mL / m³. 3 .

[0070] The method for establishing the correlation model between environmental parameters and disease occurrence includes,

[0071] Collect historical environmental data and concurrent disease testing results;

[0072] Key environmental factors were analyzed using the random forest algorithm;

[0073] Determine the dose-response relationship between ammonia concentration, relative humidity, and PRV positivity.

[0074] Example 2

[0075] like Figures 2-14 As shown, this embodiment discloses an odor purification device, which is applied in Embodiment 1. It includes a shell 1, a microbial treatment component and a nutrient solution delivery component. The microbial treatment component and the nutrient solution delivery component are both disposed inside the shell 1. The microbial treatment component includes a treatment box 11 and a plurality of microbial treatment modules. The plurality of microbial treatment modules are installed vertically at intervals in the treatment box 11 and are interconnected.

[0076] The nutrient solution delivery assembly is used to deliver nutrient solution to several of the microbial treatment modules;

[0077] It also includes a pre-filter assembly, which is connected to the microbial treatment assembly. The odor from the pig farm is delivered to the pre-filter assembly by an air pump. The odor is filtered out by the pre-filter assembly and then enters the microbial treatment assembly for treatment. Finally, it is discharged through the exhaust pipe 2.

[0078] In another embodiment of the present invention, the microbial treatment module includes a support plate 10 and a packing material 9. The support plate 10 is horizontally installed in the treatment box 11. The support plate 10 is covered with ventilation holes. The packing material 9 is laid on the upper end face of the support plate 10. Microorganisms that can treat odors are attached to the packing material 9.

[0079] It should be noted that the microorganisms in this embodiment can specifically be:

[0080] Bacillus subtilis: decomposes nitrogenous organic matter in odorous gases and reduces ammonia release.

[0081] Nitrifying bacteria (such as nitrite-oxidizing bacteria and nitrate-oxidizing bacteria): convert ammonia (NH3) into nitrate (NO3). - This reduces the ammonia concentration.

[0082] Photosynthetic bacteria (such as Rhodopseudomonas): absorb ammonia and hydrogen sulfide, while promoting the decomposition of organic matter.

[0083] Sulfur-oxidizing bacteria (such as Thiobacillus): oxidize hydrogen sulfide (H2S) to sulfate (SO4). 2- ).

[0084] Sulfate-reducing bacteria (such as Desulfovibrio): indirectly participate in the sulfur cycle under anaerobic conditions, reducing the accumulation of hydrogen sulfide.

[0085] Yeast (such as Candida): breaks down carbohydrates in odors, reducing fermentation odors.

[0086] Lactic acid bacteria: inhibit the growth of putrefactive bacteria and reduce odor production.

[0087] In another embodiment of the present invention, the nutrient solution delivery assembly includes a delivery pump 5, a storage tank 4, and a plurality of spraying mechanisms. The storage tank 4 and the delivery pump 5 are installed inside the outer casing 1. The delivery pump 5 is connected to the storage tank 4. The storage tank 4 stores nutrient solution. The plurality of spraying mechanisms are respectively installed at the upper end of the microbial treatment module. The delivery pump 5 is connected to the plurality of spraying mechanisms through a delivery pipe 6. Specifically, the spraying mechanism includes a spray plate 7 and a plurality of spray heads 8. The spray plate 7 is connected to the delivery pipe 6. The plurality of spray heads 8 are installed on the lower end face of the spray plate 7. The nutrient solution in the storage tank 4 is delivered to the spray plate 7 by the delivery pump 5 through the delivery pipe 6 and sprayed out from the spray heads 8.

[0088] In another embodiment of the present invention, the pre-filter assembly includes a filter box 3 and several filter modules. The filter box 3 has several partition plates 39, which divide the interior of the filter box 3 into several filter chambers 12. Several mounting slots 36 are provided at the upper end of the filter box 3 corresponding to the positions of the filter chambers 12. The filter modules can pass through the mounting slots 36 into the filter chambers 12 to filter the odorous gas passing through them. Since the impurities in the odorous gas entering the filter box may be unevenly distributed, if a traditional single-sided filter screen is used, part of the screen may be blocked while the rest can still filter normally. Therefore, in this solution, the filter box is divided into multiple filter chambers by partition plates, and each filter chamber uses a separate filter module. When one filter module is blocked, only that module needs to be replaced, and the remaining filter modules can still work normally, effectively improving work efficiency.

[0089] In this embodiment, the filter module includes a mounting frame 26 and a filter screen 15. The filter screen 15 is mounted on the mounting frame 26. The mounting groove 36 has mounting holes on both sides. A positioning component is installed in the mounting hole. The positioning component includes a spring 37 and a positioning rod 38. One end of the spring 37 is fixedly connected to the bottom of the mounting hole, and the other end is fixedly connected to the positioning rod 38. The end of the positioning rod 38 away from the spring 37 is hemispherical. The mounting frame 26 has hemispherical grooves on both sides that cooperate with the positioning rod 38. In this solution, during installation, the mounting frame is first inserted into the mounting slot, and then the entire filter module is pushed down until the upper end of the mounting frame contacts the positioning rod. As the mounting frame is pressed down, the positioning rod is pushed back into the mounting hole until the hemispherical groove on the mounting frame is aligned with the positioning rod. At this point, the positioning rod extends from the mounting hole and engages with the hemispherical groove under the action of the spring, thus completing the initial positioning of the filter module. During disassembly, simply pull the mounting frame upwards. Under the pulling force, the mounting frame pushes the positioning rod back into the mounting hole, allowing the filter module to be removed. The filter module is easy to install and remove.

[0090] Furthermore, the filtration module also includes a blockage detection mechanism, which includes a rotating plate 14, a rotating shaft 35, a mating plate 13, and an indicator plate 34. The rotating shaft 35 is rotatably mounted in the filter chamber 12 via a torsion spring. One end of the rotating plate 14 is fixedly connected to the outer circumferential surface of the rotating shaft 35, and the rotating plate 14 can rotate together with the rotating shaft 35. The mating plate 13 is disposed on the cavity wall of the filter chamber 12 away from the rotating shaft 35, and the rotating plate 14 can cooperate with the mating plate 13 to close the filter chamber 12. The upper end of the rotating shaft 35 extends through the filter box 3 and out of the groove filter box 3. The indicator plate 34 is disposed at the upper end of the rotating shaft 35. Specifically, when the filter screen is not clogged, the odor entering the filter box has a certain velocity (i.e., the odor has a certain kinetic energy), which exerts a force on the rotating plate, causing it to rotate around the shaft until the rotating plate is aligned with the flow direction of the odor (the force of the torsion spring is relatively small compared to the force generated by the kinetic energy of the odor). At this time, the shaft causes the indicator plate outside the filter box to rotate to the same direction as the flow direction of the odor (the indicator plate being in this state indicates that the filter module is in normal working condition). When the filter screen in a filter module within a filter chamber is clogged, the amount of air passing through that filter screen is greatly reduced, that is, the force acting on the rotating plate is greatly reduced, when it is less than... When the torsion spring is applied, it drives the rotating plate to rotate in the opposite direction until one end of the rotating plate contacts the mating plate. At this time, the rotating shaft drives the indicator plate outside the filter box to rotate in a direction perpendicular to the flow direction of the odor (the indicator plate being in this state indicates that the filter module in the corresponding filter chamber is blocked). At this time, the staff can replace the filter module in the corresponding filter chamber according to the indication of the indicator plate. It should be noted that the torsion spring in this embodiment can provide a torque to the rotating shaft and provide a reaction force when the rotating plate is pushed by the odor. When the filter module is not blocked, the pushing force of the odor is greater than this force, and when the filter module is blocked, the pushing force of the odor is less than this force.

[0091] It should be noted that, in order to ensure that when replacing a filter module in a clogged filter chamber, that filter chamber is blocked, preventing odors from passing through, while the filter modules in the remaining filter chambers can continue filtering uninterruptedly, in this embodiment, the filter module further includes a second positioning component. The second positioning component includes a drive rod 19, a second positioning rod 21, a limiting plate 17, a second spring 18, and three gears 23. The upper surface of the filter box 3 is provided with a second mounting hole 25. The drive rod 19 is movably installed within the second mounting hole 25. The limiting plate 17 is located on the upper end of the drive rod 19. The second spring 18 is fitted onto the drive rod 19, with one end fixed to the upper surface of the filter box 3. One end is fixedly connected to the other end, and the other end is fixedly connected to the lower end face of the limiting plate 17. The three gears 23 mesh with each other and are rotatably installed in the filter box 3 near the mounting hole 25. The drive rod 19 is provided with a number of transmission teeth 24 that can mesh with the gears 23. The filter box 3 is provided with a mounting hole 20 near the mating plate 13. The positioning rod 21 is movably disposed in the mounting hole 20. The positioning rod 21 can extend out of the mounting hole 20 and engage with the slot 40 provided on the upper end of the rotating plate 14. The positioning rod 21 is provided with a number of transmission teeth 22 that can mesh with the gears 23. The mounting frame 26 is provided with a pressure plate 16 that can cooperate with the limiting plate 17.

[0092] In the above scheme, when the filter module in the filter chamber is working normally, spring one is compressed, and positioning rod two is fully retracted into mounting hole two. When the filter module in a certain filter chamber is blocked, the amount of gas passing through that filter chamber is greatly reduced. The rotating plate rotates under the action of the torsion spring and contacts the mating plate to seal the filter chamber. The operator removes the filter module in the corresponding filter chamber through the indicator plate. During the removal of the filter module, the pressure plate moves upward with the filter module and no longer exerts downward pressure on the limiting plate. Therefore, the drive rod moves upward under the action of spring two, thereby driving the three gears one to rotate. During the rotation of gear one, the positioning rod two moves downward and extends out of mounting hole three 20 to engage with the slot one 40 set at the upper end of the rotating plate 14, so that the filter module can be completely removed. The positioning rod one provides a limit to the rotation of the rotating plate, preventing the rotating plate from being pushed open by the kinetic energy of the odorous gas after the blocked filter module is removed and entering the subsequent processing components, thereby ensuring that the filter modules in the remaining filter chambers can operate normally. It should be noted that, due to the excessive The lower part of the mounting frame of the filter module engages with the limiting groove on the lower end face of the filter chamber. Therefore, when the clogged filter module is removed and moves upward, the lower end of the mounting frame must be completely disengaged from the limiting groove before the gas in the filter chamber can pass through the lower end of the mounting frame. Therefore, in this application, the depth of the limiting groove is slightly greater than the distance that the spring 2 drives the drive rod to move upward. This ensures that the positioning rod 1 can be engaged in the slot 1 to rotate and position the rotating plate before the gas in the filter chamber passes through the lower end of the mounting frame, preventing the rotating plate from being pushed open by the gas before it is positioned. Through the technical solution of this embodiment, on the one hand, the rotating plate drives the rotating shaft to rotate, thereby driving the indicator plate to rotate to indicate that the filter module in the corresponding filter chamber is clogged and needs to be replaced. On the other hand, when replacing the filter module, the positioning component 2 rotates and positions the rotating plate to block the filter chamber, preventing odor from mixing with the odor in other filter chambers without being filtered, thus affecting the overall filtration effect.

[0093] As those skilled in the art will know, the device may vibrate during operation. During the vibration, the positioning of the first positioning component may fail under the elastic force of the second spring, causing the filter module to be lifted by the second spring and resulting in the positioning failure of the first positioning component. Therefore, in this embodiment, the filter box 3 is also provided with a third positioning component to lock the lower end of the filter module. The third positioning component is connected to the rotating shaft 35 through a transmission component. The transmission assembly includes gear 2 42, gear 3 44, gear 43, and gear 5 30. Gear 2 42 is located at the lower end of the rotating shaft 35 and can rotate with the rotating shaft 35. Gear 2 42, gear 3 44, gear 43, and gear 5 30 mesh and transmit power sequentially. The positioning assembly includes positioning rod 3 32, spring 3 31, and transmission rod 29. The bottom of the filter chamber 12 is provided with a limiting groove 33 that cooperates with the mounting frame 26. The groove wall of the limiting groove 33 is provided with mounting hole 4 28. The positioning assembly is installed in mounting hole 4 28. One end of spring 3 31 is fixedly connected to positioning rod 3 32, and the other end is fixedly cooperated with transmission rod 29. One end of positioning rod 3 32 can extend into the limiting groove 33 and engage with slot 2 27 at the end of the mounting frame 26. The transmission rod 29 is provided with several transmission teeth 3 41, which mesh and transmit power with gear 5 30.

[0094] In the above scheme, when the kinetic gas drives the rotating plate and the rotating shaft to rotate, gear two on the rotating shaft drives gear three (44), gear four (43), and gear five to rotate synchronously. Gear five drives positioning rod three to extend from mounting hole four and engage with slot two on the mounting frame, thereby locking the lower end of the mounting frame during operation and preventing the filter module from being pushed up by spring two. When the filter screen is blocked, the rotating plate and the rotating shaft rotate in the opposite direction, which in turn drives gear five to rotate in the opposite direction, causing positioning rod three to disengage from slot two and retract into mounting hole four. In this embodiment, it should be noted that positioning rod three will only disengage from slot two and retract into mounting hole four when the rotating plate rotates to completely block the filter cavity with the mating plate. This effectively prevents the filter module from being removed by the operator if the filter cavity is not completely blocked, thereby preventing odor from passing through the filter cavity without being filtered.

[0095] The above description only illustrates the preferred embodiments of the present invention and should not be construed as limiting the scope of the claims. The present invention is not limited to the above embodiments, and variations in its specific structure are permitted. All modifications made within the scope of the independent claims of this invention are also within the scope of protection of this invention.

Claims

1. A method for purifying porcine pseudorabies GE, characterized in that: The purification method includes the following steps: S1. Baseline environmental assessment and odor purification device installation in pig farms: Test the air quality in pig houses and measure the concentrations of ammonia, hydrogen sulfide, and carbon dioxide; at the same time, install odor purification devices in the ventilation system of pig houses; establish a real-time environmental parameter monitoring system to maintain ammonia concentration <10ppm and hydrogen sulfide concentration <5ppm. S2. Swine herd health status assessment and immunization program optimization: Multiplex quantitative PCR was used to detect PRV wild-type virus, vaccine strain, and porcine circovirus; ELISA was used to detect GB and GE antibodies; differentiated immunization programs were developed based on the test results; and an immunization efficacy evaluation system was established. S3. Disease purification and odor control: The isolation pens for positive pigs are equipped with independent odor purification units and ozone generation modules; at the same time, a tiered elimination strategy is adopted; the disinfection of the pigsty adopts an alternating scheme of "dry fog hydrogen peroxide - micro-electrolysis of water", and the odor purification device is activated simultaneously during disinfection; S4. Environmental-Disease Linkage Monitoring and Early Warning: Establish a correlation model between environmental parameters and disease occurrence; activate enhanced monitoring procedures when ammonia concentration is >15ppm for 3 consecutive days; develop a mobile APP to display environmental data and disease risk levels in real time; S5. Purification effect maintenance: Environmental microbial testing is conducted monthly, with a focus on monitoring PRV load in aerosols; the microbial agent in the odor purification device is replaced quarterly; newly introduced breeding pigs must undergo a three-stage procedure of "isolation-testing-odor adaptation".

2. The method for purifying porcine pseudorabies GE according to claim 1, characterized in that: The differential immunization procedure in S2 includes, GE antibody-negative pig herds: Primary immunization with gene-deleted vaccines; For GE antibody-positive pigs: strengthen immunization and use interferon inducers.

3. The method for purifying porcine pseudorabies GE according to claim 1, characterized in that: The immune efficacy evaluation system in S2 uses a neutralizing antibody titer ≥1:16 as the protection standard.

4. The method for purifying porcine pseudorabies GE according to claim 1, characterized in that: The tiered elimination strategy in S3 includes: Observation of markers in pigs that tested positive for the first time; Pigs that test positive a second time will be immediately culled and transported to the biosafety processing area via a dedicated channel.

5. The method for purifying porcine pseudorabies GE according to claim 1, characterized in that: The "dry fog hydrogen peroxide-micro-electrolysis water" disinfection scheme in S3 is as follows: Use hydrogen peroxide dry fog disinfection once a week, with an action time of 60 minutes; Disinfect by spraying micro-electrolyzed water every two weeks.

6. The method for purifying porcine pseudorabies GE according to claim 5, characterized in that: The hydrogen peroxide is 5% hydrogen peroxide.

7. The method for purifying porcine pseudorabies GE according to claim 5, characterized in that: The pH value of the micro-electrolyzed water is 2.

5.

8. The method for purifying porcine pseudorabies GE according to claim 7, characterized in that: The dosage of the micro-electrolyzed water is 10 mL / m 3 .

9. The method for purifying porcine pseudorabies GE according to claim 1, characterized in that: The method for establishing the correlation model between environmental parameters and disease occurrence includes, Collect historical environmental data and concurrent disease testing results; Key environmental factors were analyzed using the random forest algorithm; Determine the dose-response relationship between ammonia concentration, relative humidity, and PRV positivity.

10. A method for purifying porcine pseudorabies GE according to claim 1, characterized in that: The odor purification device in S1 includes a shell, a microbial treatment component, and a nutrient solution delivery component. The microbial treatment component and the nutrient solution delivery component are both located inside the shell. The microbial treatment component includes a treatment box and several microbial treatment modules. The several microbial treatment modules are installed vertically at intervals inside the treatment box and are interconnected. The nutrient solution delivery component is used to deliver nutrient solution to the several microbial treatment modules.

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