Zebra fish toxicology exposure experiment control system

Abstract

The invention relates to the technical field of toxicology experiment equipment, and discloses a zebra fish toxicology exposure experiment control system, which comprises a sample storage module, a culture module, a sampling and cleaning module, a water purification module, a waste liquid module, a data acquisition module and a control module, and the control module controls the operation of each module according to the data acquired by the data acquisition module. Through the synergistic effect of multiple modules, automation is achieved, and the problems of high manpower, high material resources and large operation errors are solved.

Description

Technical Field

[0001] This invention relates to the field of toxicology experimental equipment technology, and more specifically, to a zebrafish toxicology exposure experimental control system. Background Technology

[0002] Zebrafish are an important model organism for toxicological research and are widely used in cutting-edge fields such as environmental toxicology and drug safety evaluation.

[0003] However, the traditional manual operation mode has many drawbacks in the implementation of zebrafish toxicology exposure experiments. Currently, the replacement of exposure solutions and sample collection in the experimental process all rely on manual operation by the experimenters. Each time the exposure solution is replaced, the staff needs to perform operations such as pouring out the solution, cleaning, preparing and adding fresh solution to each culture container in sequence. The steps are cumbersome, time-consuming, and prone to human error, which can lead to deviations in solution concentration and affect the accuracy and reliability of experimental data.

[0004] In terms of sample collection, researchers need to frequently come into contact with the experimental subjects, which not only increases the risk of cross-contamination, but also increases the cost of experiments due to the need for a continuous investment of a large amount of manpower.

[0005] In long-term exposure experiments, as the experimental period lengthens, the repetitive manual labor not only reduces work efficiency and further increases operational errors, but also becomes a bottleneck restricting the improvement of efficiency and quality in zebrafish toxicology research, which urgently needs to be overcome through technological innovation.

[0006] Therefore, it is necessary to design a zebrafish toxicology exposure experimental control system to solve the above problems. Summary of the Invention

[0007] In view of this, the present invention proposes a zebrafish toxicology exposure experiment control system, which aims to solve the problems of high labor costs, high material consumption, and large operational errors faced by manual replacement of exposure solutions and sampling in existing zebrafish toxicology exposure experiments.

[0008] This invention proposes a control system for zebrafish toxicological exposure experiments, comprising:

[0009] The sample preservation module includes sample vials, sample vial ports, and sample preservation racks;

[0010] Aquaculture module for culturing zebrafish samples;

[0011] The sampling and cleaning module is used to put zebrafish samples from the aquaculture module into the sample preservation module, and is also used to clean the aquaculture module and perform its own cleaning.

[0012] The water purification module is used to store purified water and transmit it to the aquaculture module;

[0013] Waste liquid module, used to transfer and store waste liquid from the sampling and cleaning module;

[0014] The data acquisition module is used to collect real-time data on water level and outflow from the water purification module, water volume from the waste liquid module, and concentration from the aquaculture module.

[0015] The control module is connected to the water purification module, waste liquid module, aquaculture module, sample preservation module, sampling and cleaning module, and data acquisition module, and controls the operation of each module.

[0016] Furthermore, the sampling and cleaning module includes a sampling syringe unit and a sampling arm unit;

[0017] The sampling arm unit is used to drive the sampling syringe unit to move;

[0018] The sampling syringe unit is used to draw up waste liquid and samples, and also for self-cleaning.

[0019] Furthermore, the water purification module includes a water purification tank, water purification pipes, and a cleaning water tank;

[0020] The purified water in the water purification tank is transported to the aquaculture module through a water purification pipeline;

[0021] The cleaning tank is used to store clean water.

[0022] Furthermore, the waste liquid module includes a waste liquid tank, a waste liquid pipeline, and a wastewater tank;

[0023] The wastewater tank is used to hold the waste liquid after the sampling syringe unit has been cleaned.

[0024] The waste liquid pipeline is used to transport the waste liquid drawn up by the sampling syringe unit to the waste liquid tank for storage.

[0025] Furthermore, the control module includes a switching unit, a peristaltic pump, a solenoid valve, an alarm unit, an analysis unit, a temperature control unit, and a timing unit;

[0026] The switching unit is used to control the switching of the sampling syringe unit and the movement of the sampling arm unit.

[0027] The peristaltic pump is used to control the waste liquid discharge process of the sampling syringe unit;

[0028] The solenoid valve is used to control the amount of water supplied to the aquaculture module through the water purification pipeline;

[0029] The alarm unit is used to issue an alarm.

[0030] The analysis unit is used to receive and record the data collected by the data acquisition module;

[0031] The temperature control unit is used to control the temperature of the aquaculture module;

[0032] The timing unit is used to preset the duration.

[0033] Furthermore, the control module is used to control the sampling and cleaning module to take samples from the aquaculture module and place them in the sample preservation module.

[0034] Furthermore, the control module is also used to control the sampling and cleaning module to change the liquid in the aquaculture module.

[0035] Furthermore, the control module is also used to calculate the single waste liquid discharge volume and single water replenishment volume during the liquid replacement of the sampling and cleaning module.

[0036] Furthermore, the control module is also used to control the waste liquid discharge process of the sampling syringe unit.

[0037] Furthermore, the control module is also used to generate an alarm based on the data information.

[0038] Furthermore, the data information includes: water level data in the water purification tank and cleaning tank, water flow rate of the water purification pipe, water volume data in the waste liquid tank and wastewater tank, and concentration data of the aquaculture module.

[0039] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0040] This invention connects and controls the sample preservation module, aquaculture module, sampling and cleaning module, water purification module, and waste liquid module through a control module. This eliminates the need for manual operation in zebrafish toxicology exposure experiments; instead, the modules work together automatically, reducing labor costs. The data acquisition module collects data in real time, avoiding concentration deviations and inaccurate sampling times caused by manual operation, improving the repeatability and accuracy of experimental operations, and ensuring the reliability of experimental data. The sampling and cleaning module automatically completes sampling and self-cleaning operations, reducing the need for personnel to come into contact with the experimental subjects. This reduces the risk of cross-contamination from manual operation and ensures high-quality experimental results.

[0041] This invention uses a control module to issue alarms, enabling experimenters to promptly detect abnormalities in system operation, thereby improving the safety and reliability of experiments. Attached Figure Description

[0042] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings:

[0043] Figure 1 A functional block diagram of a zebrafish toxicology exposure experiment control system provided in an embodiment of the present invention;

[0044] Figure 2 A three-dimensional control system for zebrafish toxicological exposure experiments provided in this embodiment of the invention. Figure 1 Structural diagram;

[0045] Figure 3 A three-dimensional control system for zebrafish toxicological exposure experiments provided in this embodiment of the invention. Figure 2 Structural diagram.

[0046] Wherein: 1-Switch unit; 2-Clean water tank; 3-Waste liquid tank; 4-Waste liquid pipeline; 5-First Z-axis sampling arm; 6-First Y-axis sampling arm; 7-First sampling syringe; 8-X-axis sampling arm; 9-Second Z-axis sampling arm; 10-Second Y-axis sampling arm; 11-Second sampling syringe; 12-Clean water pipeline; 13-Cleaning water tank; 14-Solenoid valve; 15-Waste water tank; 16-Aquaculture water tank; 17-Sample storage rack; 18-Sample bottle port; 19-Peristaltic pump; 20-Sample bottle. Detailed Implementation

[0047] Exemplary embodiments of the present disclosure will now be described in more detail with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the disclosure to those skilled in the art. It should be noted that, unless otherwise specified, embodiments and features in the embodiments of the present invention can be combined with each other. The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0048] See Figure 1-3 As shown in the figure, an embodiment of the present invention proposes a control system for zebrafish toxicological exposure experiments, including: a sample preservation module, a culture module, a sampling and cleaning module, a water purification module, a waste liquid module, a data acquisition module, and a control module.

[0049] Specifically, the sample preservation module includes a sample bottle 20, a sample bottle aperture 18, and a sample preservation rack 17. The sample bottle 20 is used to hold zebrafish samples or exposed solution samples collected from the aquaculture module. The sample bottle aperture 18 is a pre-set positioning hole on the sample preservation rack 17, used to fix the sample bottle 20 and ensure accurate positioning during sampling. The sample preservation rack 17 has a cooling function and can maintain a low temperature environment, such as 4°C. The sample preservation rack 17 is used to preserve samples and prevent sample deterioration.

[0050] Specifically, the aquaculture module includes multiple aquaculture tanks 16, which are used to culture zebrafish samples to provide live experimental subjects for toxicology experiments.

[0051] Specifically, the sampling and cleaning module is used to place zebrafish samples from the aquaculture module into the sample preservation module, and also to clean the aquaculture module and perform its own cleaning. The sampling and cleaning module includes a sampling syringe unit and a sampling arm unit; the sampling syringe unit includes a first sampling syringe 7 and a second sampling syringe 11; the sampling arm unit includes: an X-axis sampling arm 8, a first Y-axis sampling arm 6, a first Z-axis sampling arm 5, a second Y-axis sampling arm 10, and a second Z-axis sampling arm 9.

[0052] The X-axis sampling arm 8, the first Y-axis sampling arm 6, and the first Z-axis sampling arm 5 respectively drive the first sampling injector 7 to move in the X-axis, Y-axis, and Z-axis directions; the X-axis sampling arm 8, the second Y-axis sampling arm 10, and the second Z-axis sampling arm 9 respectively drive the second sampling injector 11 to move in the X-axis, Y-axis, and Z-axis directions.

[0053] The first sampling syringe 7 is used to draw up the waste liquid in the aquaculture module and discharge the waste liquid into the waste liquid module; the second sampling syringe 11 is used to draw up the sample in the aquaculture module and place the sample into the sample bottle 20; the first sampling syringe 7 and the second sampling syringe 11 are also used to perform self-cleaning and discharge the waste liquid after self-cleaning into the waste liquid module.

[0054] As can be seen, the sampling and cleaning module breaks down the sampling function into a syringe execution unit and a robotic arm positioning unit, realizing a modular design that facilitates maintenance and upgrades. The multi-axis motion structure of the robotic arm unit can precisely control the sampling position and path, while the syringe unit ensures accurate sampling volume. The two work together to improve the accuracy and repeatability of the sampling operation and reduce sample contamination or volume errors caused by human operation.

[0055] Specifically, the water purification module is used to store purified water and transmit it to the aquaculture module. The water purification module includes a water purification tank 2, a water purification pipe 12, and a cleaning tank 13; the water purification tank 2 is used to store purified water; the water purification pipe 12 is used to transport the purified water in the water purification tank 2 to the aquaculture module; the cleaning tank 13 is used to store purified water and clean the first sampling syringe 7 and the second sampling syringe 11.

[0056] As can be seen, the water purification tank 2 can provide a stable water source, the water purification pipe 12 can realize directional water delivery, and the cleaning water tank 13 is used for cleaning the sampling syringe and the breeding module. The three of them constitute a complete water purification supply and cleaning system. The amount of water delivered can be controlled as needed, such as adding a quantitative amount of water when changing the water. At the same time, the sampling syringe is pre-cleaned through the cleaning water tank to avoid cross-contamination and ensure the cleanliness of the experimental environment.

[0057] Specifically, the waste liquid module is used to transfer and store the waste liquid in the sampling and cleaning module; the waste liquid module includes a waste liquid tank 3, a waste liquid pipeline 4, and a wastewater tank 15. The wastewater tank 15 is used to hold the waste liquid after the first sampling syringe 7 and the second sampling syringe 11 have cleaned themselves; the waste liquid pipeline 4 is used to transport the waste liquid drawn by the first sampling syringe 7 to the waste liquid tank 3 for storage.

[0058] As can be seen, the wastewater tank 15 temporarily collects cleaning waste liquid, and the waste liquid pipeline 4 uniformly transports the waste liquid to the waste liquid tank 3 for storage, forming a closed-loop waste liquid treatment system; the waste liquid tank 3 can centrally manage the waste liquid, avoiding the trouble of waste liquid overflow or decentralized treatment, and at the same time, it works with the data acquisition module to monitor the amount of waste liquid, which is convenient for timely cleaning, ensuring the safety of the experimental environment and preventing the spread of pollution.

[0059] Specifically, the data acquisition module includes multiple sensors, which are respectively configured in the water purification module, waste liquid module, and aquaculture module, for real-time collection of water level data and outflow data of the water purification module, water volume data of the waste liquid module, and concentration data of the aquaculture module.

[0060] It can be seen that the data acquisition module monitors key parameters in real time, providing decision-making basis for the control module and ensuring the stability of the experimental environment.

[0061] Specifically, the control module is connected to the water purification module, waste liquid module, aquaculture module, sample preservation module, sampling and cleaning module, and data acquisition module, respectively.

[0062] Specifically, the control module includes a switch unit 1, a peristaltic pump 19, a solenoid valve 14, an alarm unit, an analysis unit, a temperature control unit, and a timing unit.

[0063] Specifically, the switching unit 1 is used to control the switching of the first sampling syringe 7 and the second sampling syringe 11, as well as the movement of the sampling arm unit; the peristaltic pump 19 is used to control the waste liquid discharge process of the first sampling syringe 7; the solenoid valve 14, which is located at the outlet of the purified water pipe 12 near the aquaculture tank 16, and there are multiple solenoid valves 14, is used to control the amount of water supplied to the aquaculture module by the purified water pipe 12; the alarm unit is configured in the waste liquid tank 3, the wastewater tank 15, the purified water tank 2, the cleaning tank 13, and the aquaculture tank 16, and is used to issue an alarm; the analysis unit is used to receive and record the data collected by the data acquisition module; the temperature control unit is used to control the temperature of the aquaculture module; and the timing unit is used to preset the duration.

[0064] Specifically, the process by which the control module controls the sampling and cleaning module to take samples from the aquaculture module and place them in the sample preservation module is as follows: the control module controls the sampling and cleaning module to take samples from the aquaculture module according to a preset time, and the timing unit counts down according to the preset time. After each countdown is completed, the switch unit 1 controls the second sampling syringe 11 to start taking samples from the aquaculture module.

[0065] Understandably, during sampling, the switch unit 1 is activated, and the second sampling injector 11 is moved in the X-axis, Y-axis, and Z-axis directions respectively by the X-axis sampling arm 8, the second Y-axis sampling arm 10, and the second Z-axis sampling arm 9. The three robotic arms first move the second sampling injector 11 to the cleaning tank 13 to draw a certain amount of cleaning liquid, and then move it to the wastewater tank 15 to discharge the cleaning liquid into the wastewater tank 15, thereby completing the self-cleaning of the second sampling injector 11. Then, the second sampling injector 11 is moved to the aquaculture tank 16 to draw samples.

[0066] It is understandable that sample bottles 20 are pre-placed in the sample storage rack 17. The second sampling syringe 11 is driven by the moving robotic arm to inject the sample into the sample bottle 20. After each sampling is completed, the second sampling syringe 11 is cleaned once before the next sample is drawn.

[0067] Understandably, by automating the sampling process, the risk of cross-contamination is reduced by replacing frequent manual contact with samples; the control module triggers sampling according to a preset time interval, ensuring accurate sampling time points; and storing samples in a low-temperature storage rack can maintain sample activity, providing reliable samples for subsequent toxicological analysis.

[0068] Specifically, the analysis unit receives and records in real time the water level data in the water purification tank 2 and the cleaning tank 13, the water flow rate of the water purification pipe 12, the water volume data in the waste liquid tank 3 and the waste water tank 15, and the concentration data of the aquaculture module collected by the data acquisition module each time. The analysis unit is also used to preset the water level threshold range of the water purification tank 2, the water level threshold range of the cleaning tank 13, the water volume threshold of the waste liquid tank 3, the water volume threshold of the waste water tank 15, and the concentration threshold of the aquaculture module. It also presets the sampling height of the sampling arm unit.

[0069] Specifically, the control module is also used to control the sampling and cleaning module to change the liquid in the aquaculture module, and to calculate the single waste liquid discharge volume and single water replenishment volume when the sampling and cleaning module changes the liquid.

[0070] Specifically, the control module controls the sampling and cleaning module to change the liquid in the aquaculture module through the water purification module and the waste liquid module based on water level data, water volume data, concentration data, and preset water level threshold range, water volume threshold, and concentration threshold.

[0071] Specifically, when the concentration data of the aquaculture module reaches a preset concentration threshold, the switching unit 1 controls the first sampling syringe 7 to start drawing waste liquid from the aquaculture module and discharging it into the waste liquid tank 3; the analysis unit calculates the single waste liquid discharge volume of the first sampling syringe 7 based on the water volume data of the waste liquid tank 3 recorded each time; the solenoid valve 14 controls the water purification pipe 12 to replenish water to the aquaculture module once based on the single waste liquid discharge volume, and the single water replenishment volume is equal to the single waste liquid discharge volume. The analysis unit calculates the opening time of the solenoid valve 14 based on the single water replenishment volume and the outflow rate of the water purification pipe 12; the solenoid valve 14 controls the single water replenishment volume delivered to the aquaculture module by controlling the opening time.

[0072] Understandably, the switch unit 1 controls the start and stop of the sampling and cleaning module, the peristaltic pump 19 adjusts the waste liquid discharge rate, and the solenoid valve 14 precisely controls the water intake; the alarm unit provides real-time early warning of abnormalities, the analysis unit processes data and generates control strategies; the temperature control unit maintains the temperature of the breeding environment, and the timing unit triggers operations according to the experimental cycle, such as timed sampling, to ensure full automation and intelligence of the process and reduce manual intervention.

[0073] Understandably, by accurately calculating the amount of waste liquid discharged and the amount of water replenished in a single operation, "equal water exchange" can be achieved, avoiding the impact of water volume fluctuations in the aquaculture module on the survival of zebrafish. Combined with the precise control of the peristaltic pump 19 and the solenoid valve 14, the amount of liquid exchange can be adjusted according to experimental needs, such as partial or full liquid exchange, to adapt to different toxicological experimental schemes and improve the system's flexibility and applicability.

[0074] Specifically, the control module is also used to control the waste liquid discharge process of the sampling syringe unit, including controlling the waste liquid discharge process of the first sampling syringe 7 through the peristaltic pump 19.

[0075] Specifically, the peristaltic pump 19 controls the waste liquid discharge rate of the first sampling syringe 7 by adjusting its rotation speed; the peristaltic pump 19 automatically adjusts its rotation speed according to the water volume data of the waste liquid tank 3 and the water volume threshold of the waste liquid tank 3. When the water volume data of the waste liquid tank 3 reaches the water level threshold of the waste liquid tank 3, the peristaltic pump 19 reduces its rotation speed; when the rotation speed of the peristaltic pump 19 decreases, the waste liquid discharge rate decreases, and when the rotation speed of the peristaltic pump 19 increases, the waste liquid discharge rate increases.

[0076] The peristaltic pump 19 controls the waste liquid discharge time of the first sampling syringe 7 by adjusting the running time; the analysis unit calculates the running time of the peristaltic pump 19 based on the water volume data of the waste liquid tank 3 and the water volume threshold of the waste liquid tank 3. When the peristaltic pump 19 stops running, the first sampling syringe 7 stops discharging waste liquid.

[0077] Understandably, the peristaltic pump 19 is used to regulate the waste liquid discharge rate to avoid excessively fast discharge causing drastic fluctuations in the water flow of the aquaculture module, or excessively slow discharge affecting experimental efficiency. The control module adjusts the discharge strategy in real time according to the capacity of the waste liquid tank 3, such as slowing down or pausing discharge, to prevent waste liquid from overflowing, ensure experimental safety, and optimize the discharge process to reduce waste liquid residue.

[0078] Understandably, when replacing the solution in the aquaculture module, the switching unit 1 is activated. First, the X-axis sampling arm 8 and the second Y-axis sampling arm 10 drive the first sampling syringe 7 to move above the aquaculture tank 16 in the X-axis and Y-axis directions, respectively. Then, the second Z-axis sampling arm 9 controls the first sampling syringe 7 to move in the Z-axis direction, slowly descending to the preset sampling height. The peristaltic pump 19 controls the first sampling syringe 7 to suck up the waste liquid in the aquaculture tank 16 by adjusting the speed and running time, and then discharges the waste liquid into the waste liquid tank 3 through the waste liquid pipe 4. The analysis unit calculates the amount of waste liquid discharged in a single operation, thereby calculating the amount of water that needs to be replenished to the aquaculture tank 16 in a single operation and the opening time of the solenoid valve 14. The solenoid valve 14 is opened, and within the opening time, the water in the water purification tank 2 is transported to the aquaculture tank 16 through the water purification pipe 12, thus completing the replacement of the aquaculture solution.

[0079] It is understandable that the sample storage rack 17 is pre-stored with a stock solution of toxic compounds. After the water is replenished, if it is necessary to add toxic compounds, the second sampling syringe 11 can be controlled by the switch unit 1 to draw up the toxic compounds and add them to the aquaculture tank 16; then the switch unit 1 controls the second sampling syringe 11 to perform a self-cleaning step.

[0080] It is understandable that the self-cleaning steps of the first sampling syringe 7 are the same as those of the second sampling syringe 11.

[0081] Specifically, the control module is also used to trigger an alarm based on the data information as follows: the control module triggers an alarm based on the water level data of the water purification tank 2 and the cleaning water tank 13, the water volume data of the waste liquid tank 3 and the waste water tank 15, and the preset water level threshold range and water volume threshold.

[0082] Specifically, when the water level data of the water purification tank 2 is lower than the water level threshold range of the water purification tank 2, or when the water level data of the cleaning water tank 13 is lower than the water level threshold range of the cleaning water tank 13, the control module will issue an audible and visual alarm and provide a voice prompt to replenish water.

[0083] When the water level data of the water purification tank 2 exceeds the water level threshold range of the water purification tank 2, and when the water level data of the cleaning water tank 13 exceeds the water level threshold range of the cleaning water tank 13, the control module will issue an audible and visual alarm and provide a voice prompt to stop water replenishment.

[0084] When the water volume data of waste liquid tank 3 reaches the water volume threshold of waste liquid tank 3, or the water volume data of waste water tank 15 reaches the water volume threshold of waste water tank 15, the control module will issue an audible and visual alarm and provide a voice prompt to discharge the waste liquid.

[0085] Understandably, when the water level in the water purifier tank is below the preset threshold, the alarm unit prompts for water replenishment; when the water level in the waste liquid tank reaches the upper limit, the alarm unit prompts for waste liquid to be cleaned to prevent overflow; and when the water level in the cleaning tank is abnormal or the waste water tank is overflowing, the alarm is also triggered.

[0086] As can be seen from the above embodiments, the present invention connects and controls the sample preservation module, aquaculture module, sampling and cleaning module, water purification module, and waste liquid module through a control module. This eliminates the need for manual operation in zebrafish toxicology exposure experiments; instead, the modules work together automatically, reducing labor costs. Real-time data acquisition through the data acquisition module avoids concentration deviations and inaccurate sampling times caused by manual operation, improving the repeatability and accuracy of experimental operations and ensuring the reliability of experimental data. The sampling and cleaning module automatically completes sampling and self-cleaning operations, reducing the need for personnel to come into contact with the experimental subjects. This reduces the risk of cross-contamination from manual operation and ensures high-quality experimental results.

[0087] This invention uses a control module to issue alarms, enabling experimenters to promptly detect abnormalities in system operation and improving the safety and reliability of experiments.

[0088] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the specific implementation of the present invention. Any modifications or equivalent substitutions that do not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A control system for zebrafish toxicological exposure experiments, characterized in that, include: The sample preservation module includes sample vials, sample vial ports, and sample preservation racks; Aquaculture module for culturing zebrafish samples; The sampling and cleaning module is used to put zebrafish samples from the aquaculture module into the sample preservation module, and is also used to clean the aquaculture module and perform its own cleaning. The water purification module is used to store purified water and transmit it to the aquaculture module; Waste liquid module, used to transfer and store waste liquid from the sampling and cleaning module; The data acquisition module is used to collect real-time data on water level and outflow from the water purification module, water volume from the waste liquid module, and concentration from the aquaculture module. The control module is connected to the water purification module, waste liquid module, aquaculture module, sample preservation module, sampling and cleaning module, and data acquisition module, and controls the operation of each module.

2. The zebrafish toxicology exposure experimental control system according to claim 1, characterized in that, The sampling and cleaning module includes a sampling syringe unit and a sampling arm unit.

3. The zebrafish toxicology exposure experimental control system according to claim 2, characterized in that, The water purification module includes a water purification tank, water purification pipes, and a cleaning water tank.

4. The zebrafish toxicology exposure experiment control system according to claim 3, characterized in that, The waste liquid module includes a waste liquid tank, waste liquid pipelines, and wastewater tank.

5. The zebrafish toxicology exposure experimental control system according to claim 4, characterized in that, The control module includes a switching unit, a peristaltic pump, a solenoid valve, an alarm unit, an analysis unit, a temperature control unit, and a timing unit.

6. The zebrafish toxicology exposure experimental control system according to claim 5, characterized in that, The control module is used to control the sampling and cleaning module to take samples from the breeding module and place them in the sample preservation module.

7. The zebrafish toxicology exposure experimental control system according to claim 6, characterized in that, The control module is also used to control the sampling and cleaning module to change the liquid in the aquaculture module.

8. The zebrafish toxicology exposure experiment control system according to claim 7, characterized in that, The control module is also used to calculate the single waste liquid discharge volume and single water replenishment volume when the sampling and cleaning module changes the liquid.

9. The zebrafish toxicology exposure experimental control system according to claim 1, characterized in that, The control module is also used to control the waste liquid discharge process of the sampling syringe unit.

10. The zebrafish toxicology exposure experimental control system according to claim 9, characterized in that, The control module is also used to generate an alarm based on the data information.

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