Extraction method of chlorella polysaccharide and application of chlorella polysaccharide in soothing and oil control products

Abstract

The invention relates to an extraction method of chlorella polysaccharide and application of the chlorella polysaccharide in relieving and oil controlling products in the field of preparation of chlorella polysaccharide, and the extraction method comprises the following steps: S1, basic extraction parameter setting operation, S2, ultrasonic treatment, S3, freezing and thawing circulation, S4, separation and purification, and S5, intelligent cooperative regulation. Real-time acquisition, dynamic analysis and regulation and control compensation of data in the whole extraction process can be realized, and a single and collaborative regulation and control compensation effect is effectively implemented for each extraction link based on a production and consumption balance principle, so that extraction parameters are accurately optimized, the energy consumption and the production cost can be remarkably reduced while the extraction efficiency is improved, and the method is suitable for industrial production. In the extraction process, a dynamic regulation and control compensation effect of extraction parameters is effectively established, so that the raw material conversion efficiency, the chlorella polysaccharide yield and the overall economic benefit can be synchronously improved through accurate process control.

Description

TECHNICAL FIELD

[0001] The present application relates to the extraction and application of Chlorella vulgaris polysaccharide, in particular to a Chlorella vulgaris polysaccharide extraction method applied to the preparation field of Chlorella vulgaris polysaccharide and its application in soothing and oil control products. BACKGROUND

[0002] Chlorella vulgaris polysaccharide extraction technology can be divided into two systems: traditional process and modern technology. The traditional method is mainly based on hot water extraction, acid and alkali extraction, and enzymatic hydrolysis, while the modern technology covers ultrasonic assisted extraction, microwave extraction, high pressure homogenization and other strengthening methods. However, these extraction technologies generally have low extraction efficiency, insufficient purity, and difficulty in adapting to the needs of industrialized mass production, which significantly restricts the economic application of Chlorella vulgaris polysaccharide.

[0003] In view of the above problems, the preparation method of Chlorella vulgaris polysaccharide in Chinese invention patent CN116023517A drives the box body together with the container to move back and forth in the freezing chamber and the heating chamber at a specific time interval by the linear moving mechanism, which can achieve the purpose of multiple automatic freeze-thaw, greatly saving the labor cost, especially for Chlorella vulgaris polysaccharide factory large-scale extraction processing, greatly improving the production efficiency.

[0004] Although the existing technology has improved the extraction efficiency of Chlorella vulgaris polysaccharide to a certain extent, the parameter regulation of the extraction process mainly depends on the experience judgment and repeated test correction of technical personnel, which limits the process control precision. This extensive regulation not only causes the double reduction of Chlorella vulgaris raw material utilization rate and Chlorella vulgaris polysaccharide extraction rate, but also increases energy consumption and weakens economic benefits. SUMMARY

[0005] In view of the above existing technology, the technical problem to be solved by the present application is how to realize precise regulation of the extraction process through intelligent technology, so as to improve the raw material conversion efficiency, Chlorella vulgaris polysaccharide yield and overall economic benefit.

[0006] In order to solve the above problems, the present application provides a Chlorella vulgaris polysaccharide extraction method, which comprises an intelligent collaborative system and an extraction production line matched therewith, the intelligent collaborative system comprising an intelligent processing end, the input end of the intelligent processing end being connected with an extraction data acquisition end, and the output end of the intelligent processing end being connected with a collaborative regulation end.

[0007] The method comprises the following steps:

[0008] S1. Basic extraction parameter setting operation,

[0009] S11. The intelligent processing end sets the extraction parameters of the extraction production line through the basic extraction parameters;

[0010] S12. The control parameters of the ultrasonic treatment, freeze-thaw cycle and separation and purification process in the production line are synergistically controlled by the synergistic control end.

[0011] S2. Ultrasonic treatment,

[0012] S21. The Chlorella raw material is mixed with the extraction solvent according to the solid-liquid ratio;

[0013] S22. The ultrasonic instrument operates according to the control instructions of the synergistic control end, and the mixed solution is treated. The ultrasonic cavitation effect destroys the cell membrane and cell wall, and releases the intracellular components;

[0014] S23. The simultaneous extraction data acquisition end collects the operating power, frequency, processing time and raw material weight of the ultrasonic instrument, and synchronizes to the intelligent processing end;

[0015] S3. Freeze-thaw cycle,

[0016] S31. The freeze-thaw cycle device operates according to the control instructions of the synergistic control end, and the mixture after ultrasonic treatment is subjected to the cycle of freezing and thawing. The ice crystal formation is used to destroy the cell structure, and the intracellular components are further released;

[0017] S32. The simultaneous extraction data acquisition end collects the freezing temperature, freezing time, thawing temperature, thawing time and cycle number of the freeze-thaw cycle device, and synchronizes to the intelligent processing end;

[0018] S4. Separation and purification,

[0019] S41. After the cycle number set by the synergistic control end is completed, the centrifuge operates according to the control instructions of the synergistic control end, and the separated liquid is collected;

[0020] S42. The column chromatography device operates according to the instructions of the synergistic control end, concentrates the separated liquid and adsorbs Chlorella polysaccharide, and elutes through different concentrations of salt solution to separate neutral Chlorella polysaccharide and acidic Chlorella polysaccharide;

[0021] S43. The column chromatography device operates according to the instructions of the synergistic control end, and the eluate containing Chlorella polysaccharide is desalted by dialysis to obtain Chlorella polysaccharide;

[0022] S44. The simultaneous extraction data acquisition end collects the rotation speed, operation time, initial separated liquid weight and separated liquid weight of the centrifuge, and collects the flow rate, salt solution concentration gradient, separation time, dialysis time and Chlorella polysaccharide weight of the column chromatography device, and synchronizes to the intelligent processing end;

[0023] S5. Intelligent synergistic control,

[0024] S51. The intelligent processing end analyzes the collected extraction data, analyzes the energy production and consumption data at each stage of the Chlorella polysaccharide extraction process, and the overall extraction energy production and consumption data, adjusts and compensates the extraction parameters according to the actual production and consumption balance data, and transmits the adjustment and compensation data to the collaborative control end;

[0025] S52. The collaborative control end uses the adjusted and compensated parameters to collaboratively control the operation data of ultrasonic treatment, freeze-thaw cycle and separation and purification, promotes the balance of Chlorella polysaccharide production and consumption, and repeats step S1;

[0026] S53. The intelligent processing end can repeat step S51 to verify the adjustment and compensation parameters according to the collected data transmitted by the extraction data collection end, and dynamically adjust the applicability according to the energy production demand.

[0027] In summary, by applying the intelligent collaborative system in the extraction method, real-time data collection, dynamic analysis and adjustment and compensation of the whole extraction process can be realized. Based on the principle of production and consumption balance, single and collaborative adjustment and compensation can be implemented for each extraction link, so as to accurately optimize the extraction parameters, improve the extraction efficiency, significantly reduce the energy consumption and production cost, effectively improve the utilization rate of Chlorella raw materials and the extraction rate of Chlorella polysaccharide, and make the extraction method support the application of batch and automatic production line. In the extraction process, the dynamic adjustment and compensation of the extraction parameters are effectively established, and then the raw material conversion efficiency, Chlorella polysaccharide yield and overall economic benefit can be simultaneously improved through precise process control. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 Extraction method flowchart of the first to third embodiments of the present application;

[0029] Figure 2 Intelligent collaborative system control logic diagram of the first to third embodiments of the present application;

[0030] Figure 3 Intelligent collaborative system acting on the extraction method flowchart of the first to third embodiments of the present application;

[0031] Figure 4 Freeze-thaw balance sensing unit operation logic diagram of the third embodiment of the present application;

[0032] Figure 5 Single freeze-thaw temperature control balance trigger time state diagram of the third embodiment of the present application;

[0033] Figure 6 Combined trigger freeze-thaw temperature control balance trigger time state diagram of the third embodiment of the present application;

[0034] Figure 7 State diagram when the single freeze-thaw temperature control imbalance abnormality triggers for the third embodiment of the present application;

[0035] Figure 8 State diagram when the combined freeze-thaw temperature control imbalance abnormality triggers for the third embodiment of the present application.

[0036] Explanation of reference numerals in the drawings:

[0037] 1 frozen temperature sensing group, 11 frozen sensing box, 12 low-temperature deformation sleeve, 13 low-temperature accompanying touch plate, 14 low-temperature balance elastic sleeve, 15 low-temperature lower limit touch block, 16 low-temperature upper limit touch block, 2 hot melt temperature sensing group, 21 hot melt sensing box, 22 high-temperature deformation sleeve, 23 high-temperature accompanying touch plate, 24 high-temperature balance elastic sleeve, 25 high-temperature upper limit touch block, 26 high-temperature lower limit touch block, 3 eccentric balance sensing group, 31 eccentric balance sleeve, 32 balance contraction tube, 33 balance sensing slider, 34 balance eccentric touch block, 35 imbalance eccentric touch block. DETAILED DESCRIPTION

[0038] The three embodiments of the present application will be described in detail below with reference to the accompanying drawings.

[0039] First embodiment:

[0040] Figure 1 - Figure 3 The extraction method of Chlorella polysaccharide is shown, which comprises an intelligent collaborative system and an extraction production line matched therewith, the intelligent collaborative system comprising an intelligent processing end, an extraction data acquisition end being connected to an input end of the intelligent processing end, and a collaborative control end being connected to an output end of the intelligent processing end.

[0041] The method comprises the following steps:

[0042] S1. Basic extraction parameter setting operation,

[0043] S11. The intelligent processing end sets the extraction parameters of the extraction production line through the basic extraction parameters;

[0044] S12. The control parameters of the ultrasonic treatment, freeze-thaw cycle and separation and purification process in the extraction production line are collaboratively controlled through the collaborative control end;

[0045] S2. Ultrasonic treatment,

[0046] S21. The Chlorella raw material is mixed with the extraction solvent according to a solid-liquid ratio of 1:10-1:30 (g / mL), and the extraction solvent can be water, alcohol or buffer;

[0047] S22. The ultrasonic instrument is operated according to the control instruction of the collaborative regulation end to process the mixed solution, the ultrasonic cavitation effect destroys the cell membrane and cell wall, and releases the intracellular components, the ultrasonic instrument has a running power of 200-800 W, a frequency of 20-40 kHz, and a processing time of 10-60 minutes;

[0048] S23. The data acquisition end simultaneously acquires data of the running power, frequency, processing time of the ultrasonic instrument, and the weight of the raw material, and synchronizes to the intelligent processing end;

[0049] S3. Freeze-thaw cycle,

[0050] S31. The freeze-thaw cycle device is operated according to the control instruction of the collaborative regulation end to freeze and thaw the mixture after ultrasonic treatment, and the ice crystal formation is used to destroy the cell structure and further release the intracellular components, the freezing temperature is-20℃ to-80℃, the freezing time is 1-12 hours, then the water bath at 37℃ is used to accelerate thawing or room temperature thawing, the freeze-thaw cycle is repeated 2-5 times, the ice crystal formation is used to destroy the cell structure and further release the components;

[0051] S32. The data acquisition end simultaneously acquires data of the freezing temperature, freezing time, thawing temperature, thawing time, freeze-thaw temperature balance data, and cycle number of the freeze-thaw cycle device, and synchronizes to the intelligent processing end;

[0052] The extraction data acquisition end can use temperature sensors to detect the freezing temperature and freeze-thaw temperature in real time, and can realize real-time detection of freeze-thaw temperature balance data through freezing temperature sensing group 1, hot thawing temperature sensing group 2, and eccentric balance sensing group 3, effectively realize effective detection and balance sensing of temperature data, to ensure the accuracy and effectiveness of temperature control in the regulation compensation process, promote the piercing rate of temperature difference to the cell wall, thereby improving the utilization rate of raw materials, reducing the influence of temperature on chlorella polysaccharide, and thereby improving the extraction rate and quality of chlorella polysaccharide;

[0053] S4. Separation and purification,

[0054] S41. After the cycle number set by the collaborative regulation end is completed, the centrifuge is operated according to the control instruction of the collaborative regulation end, the rotation speed is 4000-10,000 r / min, the separation time is 10-30 minutes, and the separated liquid is collected;

[0055] S42. The column chromatography device is operated according to the instruction of the collaborative regulation end to concentrate and adsorb chlorella polysaccharide on the separated liquid, and elute through different concentrations of salt solution to separate neutral chlorella polysaccharide and acidic chlorella polysaccharide;

[0056] S43. The column chromatography device operates according to the instructions of the collaborative regulation end to dialysis desalination of the eluent containing Chlorella polysaccharide, and an ion exchange column is prepared in the column chromatography device by using DEAE-52 cellulose, Chlorella polysaccharide in the concentrated solution is adsorbed, and Chlorella polysaccharide solution is eluted and dialyzed according to a salt solution gradient of 0.1→0.5 M NaCl linear gradient at a flow rate of 1 mL / min to obtain Chlorella polysaccharide;

[0057] S44. The extraction data acquisition end acquires data of the rotation speed, running time, initial separation liquid weight and separation liquid weight of the centrifuge, and acquires data of the flow rate, salt solution concentration gradient, separation time, dialysis time and Chlorella polysaccharide weight of the column chromatography device, and synchronizes to the intelligent processing end;

[0058] The extraction data acquisition end can realize online detection of the initial separation liquid weight, separation liquid weight and Chlorella polysaccharide weight by using a weight sensor, and the weight sensor is arranged at the corresponding position of the centrifuge and the column chromatography device to realize data acquisition, the extraction data acquisition end can realize online detection of the concentration of Chlorella polysaccharide solution in the column chromatography device by using a high-precision refractometer (±0.1% Brix), and in order to ensure detection accuracy, near-infrared spectroscopy (NIRS) can also be used for online detection, and specific applications can be selected by those skilled in the art according to actual conditions;

[0059] S5. Intelligent collaborative regulation,

[0060] S51. The intelligent processing end analyzes and processes the collected extraction data, analyzes the energy production and consumption data of each stage in the Chlorella polysaccharide extraction process, and the overall energy production and consumption data, regulates and compensates the extraction parameters according to the actual production and consumption balance data, and transmits the regulation and compensation data to the collaborative regulation end;

[0061] S52. The collaborative regulation end uses the parameters after regulation and compensation to collaboratively regulate the operation data of ultrasonic treatment, freeze-thaw cycle and separation and purification, promotes the balance of energy production and consumption of Chlorella polysaccharide, and repeats step S1, and the collaborative regulation end uses a pulse damper (±2%) to control the salt solution gradient in the column chromatography device;

[0062] S53. The intelligent processing end can subsequently collect data from the extraction data collection end, repeat step S51, verify the control compensation parameters, and dynamically adjust the applicability according to the production capacity demand. By applying an intelligent collaborative system in the extraction method, real-time data collection, dynamic analysis, and control compensation of the entire extraction process can be achieved. Based on the principle of production and consumption balance, single and collaborative control compensation can be implemented for each extraction link, thereby optimizing the extraction parameters accurately. This can improve extraction efficiency while significantly reducing energy consumption and production costs, effectively improving the utilization rate of chlorella raw materials and the extraction rate of chlorella polysaccharide. The extraction method can effectively support the adaptation of batch and automated production lines, effectively establishing dynamic control compensation of extraction parameters during the extraction process, and thus achieving simultaneous improvement of raw material conversion efficiency, chlorella polysaccharide yield, and overall economic benefits through precise process control.

[0063] Figure 3 When the intelligent processing end analyzes the production capacity and energy consumption data of each stage in the chlorella polysaccharide extraction process in step S51,

[0064] S511. The extraction data collection end collects data for each extraction step in the extraction line,

[0065] The ultrasonic operation collection unit collects data on the operating power, frequency, processing time, and raw material weight of the ultrasonic instrument; the freeze-thaw cycle collection unit collects data on the freezing temperature, freezing time, thawing temperature, thawing time, and cycle number of the freeze-thaw cycle device; the centrifugal operation collection unit collects data on the speed, operating time, initial separation liquid weight, and separation liquid weight of the centrifuge; and the chromatography data collection unit collects data on the flow rate, salt solution concentration gradient, separation time, dialysis time, and chlorella polysaccharide weight of the column chromatography device;

[0066] S512. The intelligent processing end processes and analyzes the collected data,

[0067] The ultrasonic operation collection unit, freeze-thaw cycle collection unit, centrifugal operation collection unit, and chromatography data collection unit transmit the collected data to the intelligent collaborative processing unit; the intelligent collaborative processing unit calculates the extraction energy consumption of ultrasonic treatment, freeze-thaw cycle, and separation and purification based on the obtained data; the intelligent collaborative processing unit calculates the production capacity of the centrifuge based on the initial separation liquid weight data and the separation liquid weight data, and calculates the production capacity of the column chromatography device based on the separation liquid weight and the chlorella polysaccharide weight;

[0068] The intelligent collaborative processing unit also transmits the raw material weight data in the ultrasonic treatment process and the separation liquid weight data in the separation and purification process to the reverse auxiliary analysis unit. The reverse auxiliary unit reversely reasons the combined production capacity of the ultrasonic treatment and the freeze-thaw treatment, and then feeds back the reversely reasoned combined production capacity of the ultrasonic treatment and the freeze-thaw treatment to the intelligent collaborative processing unit;

[0069] The intelligent collaborative processing unit calculates and receives data, and calculates the production and consumption related data of the ultrasonic treatment and the freeze-thaw treatment to obtain the summary production and consumption balance data of the ultrasonic treatment and the freeze-thaw treatment, and calculates the independent production and consumption balance data of the centrifuge and the column chromatography device in the separation and purification process to obtain the centrifuge production and consumption balance data and the column chromatography production and consumption balance data;

[0070] S513. The intelligent processing end regulates and compensates the analyzed data,

[0071] The intelligent collaborative processing unit transmits the calculated summary production and consumption balance data, centrifuge production and consumption balance data, column chromatography production and consumption balance data and basic extraction parameters to the collaborative compensation unit. The collaborative compensation unit specifies a single collaborative compensation effect for the ultrasonic treatment and the freeze-thaw treatment according to the summary production and consumption balance data, that is, only one of the ultrasonic treatment and the freeze-thaw treatment is parameter regulated and compensated.

[0072] The collaborative compensation unit performs synchronous collaborative compensation on the centrifuge and the column chromatography device according to the centrifuge production and consumption balance data and the column chromatography production and consumption balance data, that is, synchronous collaborative parameter regulation and compensation are performed on the centrifuge and the column chromatography device. The collaborative compensation unit feeds back the single collaborative compensation data and the synchronous collaborative compensation data to the intelligent collaborative processing unit. After processing and conversion of the data, the intelligent collaborative processing unit transmits the regulated and compensated extraction parameters to the corresponding ultrasonic operation regulation unit, freeze-thaw cycle regulation unit, centrifuge operation regulation unit and chromatography regulation unit. Through the application of the reverse auxiliary analysis unit, the reverse calculation of the production capacity of the ultrasonic treatment and the freeze-thaw cycle process can be effectively realized, and the detection of the production capacity data can be effectively ensured. Combined with the effect of the collaborative compensation unit, single and collaborative regulation and compensation of the extraction process can be realized. For different data acquisition conditions, suitable regulation and compensation of different extraction processes can be realized, and the effectiveness and precision of regulation and compensation can be effectively promoted.

[0073] Figure 3 When the regulated and compensated parameters are verified and suitable dynamic regulation is performed according to the production capacity demand in step S53,

[0074] S531. The intelligent processing end collects and analyzes the data after regulation and compensation,

[0075] The intelligent collaborative processing unit respectively collects each process data after compensation through the ultrasonic operation collection unit, the freeze-thaw cycle collection unit, the centrifugal operation collection unit and the chromatography data collection unit, analyzes and processes the data through repeated S511 to S522 steps, and then transmits the compensation parameters, the basic extraction parameters and the production and consumption balance data of each process to the feedback verification processing unit;

[0076] S532. The intelligent processing end verifies the compensation parameters,

[0077] The feedback verification processing unit verifies whether the single collaborative compensation data promotes the production and consumption balance direction, whether the synchronous collaborative compensation data promotes the production and consumption balance direction, and whether the overall extraction process promotes the production and consumption balance direction, and then feeds back the verification data to the intelligent collaborative processing unit;

[0078] S533. The intelligent processing end dynamically adjusts according to the verified data,

[0079] The intelligent collaborative processing unit processes and converts the verification data of whether each process promotes the production and consumption balance direction and whether the overall extraction process promotes the production and consumption balance direction, and then transmits these data, the compensation parameters and the compensation data of each production and consumption balance to the collaborative compensation unit, and repeats the S513 step;

[0080] The data after dynamic adjustment is: ultrasonic treatment: ultrasonic instrument operating power 600W, frequency 30kHz, processing time 20-30 minutes; freeze-thaw cycle: freezing temperature-40℃, freezing 4-6 hours, then accelerating melting with 37℃ water bath, repeating freeze-thaw cycle 3 times; separation and purification: centrifuge speed 8000r / min, separation 15 minutes, which can realize the verification effect of the compensation data effectiveness through the cooperation of the feedback verification processing unit, so as to promote the correctness of the compensation, avoid the economic loss caused by continuous compensation exception, realize the closed-loop intelligent regulation of the extraction parameters through the functions of data collection, back calculation, collaborative regulation and feedback verification, so as to promote the regulation accuracy, reduce the extraction energy consumption, realize the effect of high output and low energy consumption, and promote the economic benefit of Chlorella polysaccharide extraction.

[0081] Figure 1 - Figure 3The extraction method of Chlorella polysaccharide is shown, the extraction data acquisition end includes ultrasonic operation acquisition unit, freeze-thaw cycle acquisition unit, centrifugal operation acquisition unit and chromatography data acquisition unit; the intelligent processing end includes intelligent collaborative processing unit, collaborative compensation unit, counter-aided analysis unit and feedback verification processing unit, the intelligent collaborative processing unit is respectively connected with the collaborative compensation unit, the counter-aided analysis unit and the feedback verification processing unit signal connection;

[0082] The collaborative control end includes ultrasonic operation control unit, freeze-thaw cycle control unit, centrifugal operation control unit and chromatography control unit; the output end of the ultrasonic operation acquisition unit, the freeze-thaw cycle acquisition unit, the centrifugal operation acquisition unit and the chromatography data acquisition unit are connected with the intelligent collaborative processing unit signal connection;

[0083] The input end of the ultrasonic operation control unit, the freeze-thaw cycle control unit, the centrifugal operation control unit and the chromatography control unit are connected with the intelligent collaborative processing unit signal connection, the cooperation of the extraction data acquisition end, the intelligent processing end and the collaborative control end can effectively realize the intelligent closed-loop control effect of the extraction process, promote the intelligent regulation and compensation of the extraction parameters, improve the extraction precision, reduce the extraction energy consumption, and effectively reduce the artificial participation, can realize the dynamic regulation of the extraction parameters according to the actual data change of the extraction process, so as to effectively guarantee the regulation precision of the extraction parameters, promote the raw material conversion rate and the Chlorella polysaccharide extraction rate, effectively realize the control effect of the batch and automatic production line, and promote the economic benefit of Chlorella polysaccharide extraction.

[0084] Figure 1 - Figure 3The intelligent collaborative processing unit can utilize the centrifugal purification process productivity data and raw material initial data to reversely calculate the sum of the ultrasonic treatment and freeze-thaw cycle process productivity by the backstepping auxiliary analysis unit. Then the intelligent collaborative processing unit transmits the sum of the productivity and the corresponding energy consumption data to the collaborative compensation unit, so that the collaborative compensation unit can perform single collaborative control compensation for the ultrasonic treatment and freeze-thaw cycle. That is, the extraction process with low energy consumption is preferentially independently regulated and compensated. Then, in the subsequent application process, according to the feedback data transmitted by the feedback verification processing unit, the single collaborative control compensation effect is verified. After verification, if it is effective, the regulation or compensation is continued. If it is invalid, another extraction process is independently regulated and compensated again. Then, the feedback verification is performed again by the feedback verification processing unit. If the continuous verification is invalid, the basic extraction parameters of the ultrasonic treatment and freeze-thaw cycle are restored. If the subsequent verification is valid, the parameters of this regulation and compensation are maintained for operation. In this way, the data detection effectiveness and regulation and compensation effectiveness of the ultrasonic treatment and freeze-thaw cycle extraction processes can be realized. The backstepping detection effect on the cell wall and cell structure can be realized. The production and consumption balance can be ensured. The raw material utilization rate and the chlorella polysaccharide extraction rate are promoted while the energy consumption of the extraction process is reduced. In this way, the extraction cost is reduced and the economic benefit of chlorella polysaccharide extraction is promoted.

[0085] The intelligent collaborative processing unit also synchronously transmits the centrifugal purification process productivity and energy consumption related data to the collaborative compensation unit. The collaborative compensation unit performs collaborative regulation and compensation on the centrifuge and column chromatography device. The intelligent collaborative processing unit transmits corresponding regulation and compensation data to each control unit of the collaborative control end according to the received related regulation and compensation data. Then, the running data and productivity data after the regulation and compensation of each extraction production line process are received by the extraction data acquisition end. The regulation and compensation data are verified by the feedback verification unit. The feedback verification processing unit verifies the data of the collaborative regulation and compensation effect. The direction of the regulation and compensation after the verification is verified. If the regulation direction is effective, that is, the production and consumption balance is beneficial to productivity, the regulation and compensation is maintained or continued according to the subsequent calculation. If the regulation direction is invalid, that is, the production and consumption balance inhibits productivity, the regulation and compensation is re-regulated or the basic running parameters are restored. In this way, the regulation accuracy is promoted while the effectiveness of the production and consumption balance regulation is effectively guaranteed. The economic efficiency of chlorella polysaccharide extraction is promoted.

[0086] Figure 1 - Figure 3The extraction line shown comprises an ultrasonic instrument, a freeze-thaw cycle device arranged at the output end of the ultrasonic instrument, a centrifuge arranged at the output end of the freeze-thaw cycle device, and a column chromatography device arranged at the output end of the centrifuge; the input end of the ultrasonic operation acquisition unit is connected with the ultrasonic instrument signal, the input end of the freeze-thaw cycle acquisition unit is connected with the freeze-thaw cycle device signal, the input end of the centrifugal operation acquisition unit is connected with the centrifuge signal, and the input end of the chromatography data acquisition unit is connected with the column chromatography device signal;

[0087] The output end of the ultrasonic operation regulation unit is connected with the ultrasonic instrument signal, the output end of the freeze-thaw cycle regulation unit is connected with the freeze-thaw cycle device signal, the output end of the centrifugal operation regulation unit is connected with the centrifuge signal, and the output end of the chromatography regulation unit is connected with the column chromatography device signal. By combining the intelligent collaborative system with the extraction line, the real-time data acquisition and real-time regulation of the extraction line are realized, so as to promote the control accuracy of the extraction process, promote the production capacity of the extraction line, and effectively judge and regulate the extraction line when the production capacity of the extraction line is abnormal, reduce the generation of energy consumption, effectively inhibit the generation of abnormalities, effectively improve the automation and intelligent level of the extraction line operation, and promote the economic benefits of the extraction line operation.

[0088] Figure 1 - Figure 3The extraction data acquisition end further comprises a parameter instruction acquisition unit, the collaborative control end further comprises a collaborative data display unit and an abnormality warning unit, the input end of the parameter instruction acquisition unit is connected with the extraction production line control platform signal, the output ends of the collaborative data display unit and the abnormality warning unit are connected with the extraction production line control platform signal, and the output end of the abnormality display unit is further connected with the alarm on the extraction production line control platform, when the intelligent collaborative system is applied in the extraction method of chlorella polysaccharide, the technical personnel input the related basic parameters of chlorella polysaccharide extraction to the input end of the parameter instruction acquisition unit through the extraction production line control platform, these parameters include but are not limited to the weight of the initial raw material and the extraction solvent, the ultrasonic treatment control parameter, the freeze-thaw cycle control parameter, the separation and purification process control parameter and the corresponding start-stop instruction and other related data, the parameter instruction parameter unit transmits the converted data to the intelligent collaborative processing unit, the intelligent collaborative processing unit generates corresponding control instructions according to these parameters, and transmits corresponding control data to the ultrasonic operation control unit, the freeze-thaw cycle control unit, the centrifugal operation control unit and the chromatography control unit, so that the ultrasonic operation control unit controls the ultrasonic instrument, the freeze-thaw cycle control unit controls the freeze-thaw cycle device, the centrifugal operation control unit controls the centrifuge, and the chromatography control unit controls the column chromatography device; the ultrasonic cavitation effect is used to destroy the cell membrane and cell wall, release intracellular components, utilize the temperature difference cycle effect of freezing and thawing to promote ice crystal formation to destroy the cell structure and further release intracellular components, make the centrifuge rotate and separate to obtain a separated liquid, and obtain chlorella polysaccharide through eluent dialysis desalting technology; and in the process of regulating compensation control, the intelligent collaborative processing unit further transmits the operation data of each extraction process to the extraction production line control platform through the collaborative data display unit, so that the technical personnel can obtain the related data of the extraction process in time, intuitively and effectively, promote the three-party cooperation of the system, the technical personnel and the extraction process, and when the production and consumption balance continuously inhibits the production capacity, the intelligent collaborative processing unit further transmits the abnormal data to the extraction production line control platform through the abnormality warning unit, and starts the alarm through the abnormality warning unit to remind the technical personnel that the production capacity of the extraction production line is abnormal, so that the technical personnel can intervene in the control of the extraction production line in time, thereby avoiding the damage of the equipment and the waste of raw materials, ensuring the safety of the operation of the extraction production line, and promoting the economy of chlorella polysaccharide extraction.

[0089] The second embodiment:

[0090] Figure 1 - Figure 8The present invention illustrates a method for extracting polysaccharides from Chlorella vulgaris. The freeze-thaw cycle device includes a freezing device and a heating bath device. The freezing device is equipped with a freezing temperature sensing group 1, and the heating bath device is equipped with a heating thaw temperature sensing group 2. An eccentric balance sensing group 3 is connected between the freezing temperature sensing group 1 and the heating thaw temperature sensing group 2. The extraction data acquisition end also includes a freeze-thaw balance sensing unit. The input end of the freeze-thaw balance sensing unit is connected to the signals of the freezing temperature sensing group 1, the heating thaw temperature sensing group 2, and the eccentric balance sensing group 3, respectively. The output end of the freeze-thaw balance sensing unit is connected to the signal of the intelligent collaborative processing unit.

[0091] The intelligent processing unit also includes a freeze-thaw balance compensation unit, which is signal-connected to the intelligent collaborative processing unit. The collaborative control unit includes a freeze-thaw temperature balance control unit, whose input is signal-connected to the intelligent collaborative processing unit, and whose output is signal-connected to both the freezing device and the heating device. The setup of the freeze-thaw balance sensing unit, freeze-thaw balance compensation unit, and freeze-thaw temperature balance control unit enables real-time temperature detection during the freeze-thaw cycle. This effectively ensures the effectiveness of the production and consumption balance control of the freeze-thaw cycle device while also promoting precise control of the freeze-thaw temperature balance. While ensuring production capacity, it also reduces the impact of temperature changes on cell wall puncture rate and the quality of Chlorella polysaccharide components, effectively ensuring the quality of Chlorella polysaccharide production. Furthermore, precise temperature control promotes cell wall puncture rate and reduces energy consumption in the freeze-thaw cycle, effectively improving production capacity and raw material utilization.

[0092] Figure 4 - Figure 8 The freezing temperature sensing group 1 includes a freezing sensing box 11 fixedly installed in the freezing device. A low-temperature deformation sleeve 12 is fixedly connected to the lower inner wall of the freezing sensing box 11, and the lower end of the low-temperature deformation sleeve 12 extends to the outside of the freezing sensing box 11 and is fixedly connected to a cooling block. A low-temperature accompanying contact plate 13 that slides with the inside of the freezing sensing box 11 is fixedly connected to the upper end of the low-temperature deformation sleeve 12. A low-temperature balancing elastic sleeve 14 is fixedly connected to the upper end of the low-temperature accompanying contact plate 13, and the upper end of the low-temperature balancing elastic sleeve 14 is fixedly connected to the upper inner wall of the freezing sensing box 11. Both the low-temperature deformation sleeve 12 and the low-temperature balancing elastic sleeve 14 produce vertical expansion and contraction deformation in the freezing sensing box 11. Low-temperature lower limit contact blocks 15 located below the low-temperature accompanying contact plate 13 are fixedly connected to the left and right inner walls of the freezing sensing box 11, and low-temperature upper limit contact blocks 16 located above the low-temperature accompanying contact plate 13 are fixedly connected to the left and right inner walls of the freezing sensing box 11.

[0093] The hot melting temperature sensing group 2 comprises a hot melting sensing box 21 fixedly arranged in the hot bath device, a high-temperature deformation sleeve 22 fixedly connected to the inner wall of the hot melting sensing box 21, a heat-conducting block fixedly connected to the lower end of the high-temperature deformation sleeve 22, a high-temperature accompanying touch plate 23 fixedly connected to the upper end of the high-temperature deformation sleeve 22 and in sliding fit with the inner wall of the hot melting sensing box 21, a high-temperature balance flammable sleeve 24 fixedly connected to the upper end of the high-temperature accompanying touch plate 23 and the upper inner wall of the hot melting sensing box 21, and the high-temperature deformation sleeve 22 and the high-temperature balance flammable sleeve 24 both produce vertical extension and contraction deformation in the hot melting sensing box 21, the hot melting sensing box 21 is fixedly connected to the left and right inner walls of the hot melting sensing box 21 and is located on the upper side of the high-temperature accompanying touch plate 23, and the hot melting sensing box 21 is fixedly connected to the left and right inner walls of the hot melting sensing box 21 and is located on the lower side of the high-temperature accompanying touch plate 23.

[0094] A plurality of composite temperature sensing deformation strips are fixedly connected between the upper and lower inner walls of the low-temperature deformation sleeve 12 and the high-temperature deformation sleeve 22, the composite temperature sensing deformation strips located in the low-temperature deformation sleeve 12 can produce corresponding deformation under low-temperature triggering conditions, the composite temperature sensing deformation strips located in the high-temperature deformation sleeve 22 can produce corresponding deformation under high-temperature triggering conditions, the lower end of the composite temperature sensing deformation strip located in the low-temperature deformation sleeve 12 is fixedly connected to the cold-conducting block, and the lower end of the composite temperature sensing deformation strip located in the high-temperature deformation sleeve 22 is signal-connected to the heat-conducting block.

[0095] The eccentric balance sensing group 3 comprises an eccentric balance sleeve 31 fixedly installed outside the freezing device and the hot bath device, balance contraction pipes 32 fixedly connected to the left and right ends of the eccentric balance sleeve 31 and in communication with the eccentric balance sleeve 31, the lower end of the balance contraction pipe 32 located on the left side extends into the freezing sensing box 11 and is in communication with the low-temperature balance elastic sleeve 14, the lower end of the balance contraction pipe 32 located on the right side extends into the hot melting sensing box 21 and is in communication with the high-temperature balance elastic sleeve 24, a balance eccentric touch block 34 is fixedly connected to the left end of the inner wall of the eccentric balance sleeve 31, an unbalance eccentric touch block 35 is fixedly connected to the right end of the inner wall of the eccentric balance sleeve 31, and a balance sensing sliding block 33 is slidably arranged between the balance eccentric touch block 34 and the unbalance eccentric touch block 35 in the eccentric balance sleeve 31, the freezing temperature sensing group 1, the hot melting temperature sensing group 2 and the eccentric balance sensing group 3 can effectively realize sensing and data feedback on the actual temperature and the balance of temperature regulation in the freeze-thaw cycle process, generate a triggering signal through mechanical deformation, promote the effectiveness of the detection of the freeze-thaw temperature, combine the production and consumption balance data of the freeze-thaw process, further improve the regulation compensation accuracy of the freeze-thaw process, so as to effectively reduce the number of cycles and energy consumption while achieving high utilization of raw materials and high extraction rate of chlorella polysaccharide.

[0096] It should be noted that the composite temperature deformation strips located in the low-temperature deformation sleeve 12 and the high-temperature deformation sleeve 22 are supported by memory metal wires with multi-section temperature deformation. The composite temperature deformation strips located in the low-temperature deformation sleeve 12 can produce corresponding expansion and contraction deformation at different low temperatures. For example, since the chlorella polysaccharide is prone to degradation and activity loss under high temperature, the limit high temperature is limited to 45°C under the multiple effects of high temperature, energy consumption and hot bath time. Therefore, when the freezing temperature is set between -30°C and -50°C, the composite temperature deformation strips located in the low-temperature deformation sleeve 12 can produce extreme contraction deformation when the freezing temperature is -50°C, drive the low-temperature deformation sleeve 12 to contract to the minimum length, produce extreme expansion deformation when the freezing temperature is -30°C, drive the low-temperature deformation sleeve 12 to expand to the maximum length, and produce partial contraction deformation when the freezing temperature is -40°C, drive the low-temperature deformation sleeve 12 to contract to a certain length. The composite temperature deformation strips located in the high-temperature deformation sleeve 22 can produce corresponding expansion deformation at different high temperatures. For example, since the chlorella polysaccharide is prone to degradation and activity loss under high temperature, the limit high temperature is limited to 45°C under the multiple effects of high temperature, energy consumption and hot bath time. Therefore, when the hot bath temperature is set between 25°C and 40°C, the composite temperature deformation strips located in the high-temperature deformation sleeve 22 can produce extreme expansion deformation when the hot bath temperature is 40°C, drive the high-temperature deformation sleeve 22 to expand to the maximum length, produce extreme contraction deformation when the hot bath temperature is 25°C, drive the high-temperature deformation sleeve 22 to contract to the minimum length, and produce partial expansion deformation when the hot bath temperature is 35°C, drive the high-temperature deformation sleeve 22 to expand to a certain length.

[0097] When the composite temperature deformation passes through the temperature deformation effect to control the deformation of the low-temperature deformation sleeve 12 and the high-temperature deformation sleeve 22, respectively, the balance induction slider 33 in the eccentric balance sleeve 31 will produce corresponding accompanying movement, and when the low-temperature deformation sleeve 12 and the high-temperature deformation sleeve 22 are in the state of partial contraction and partial expansion, respectively, and the balance induction slider 33 balances the eccentric trigger block 34 to produce triggering effect, indicating that the temperature control of the freeze-thaw cycle device is in an effective and balanced state, and the temperature does not need to be adjusted and compensated;

[0098] When the low-temperature deformation sleeve 12 is in the extreme contraction state or the high-temperature deformation sleeve 22 is in the extreme expansion state, the balance induction slider 33 will continuously trigger the balance eccentric trigger block 34, indicating that the temperature control of the freeze-thaw cycle device is in a state to be adjusted and compensated, and the temperature needs to be adjusted and compensated to avoid the case that the freezing temperature is too low to cause component damage, and the case that the hot bath temperature is too high to cause the conversion of chlorella polysaccharide and reduce the polysaccharide extraction rate;

[0099] When the low-temperature deformation sleeve 12 and the high-temperature deformation sleeve 22 are in the local contraction and local elongation states respectively, and the balanced induction slider 33 triggers the unbalanced eccentric contact block 35, it indicates that the temperature control of the freeze-thaw cycle device is in an unbalanced state in the temperature control interval at this time, and temperature needs to be adjusted and compensated to avoid the continuous imbalance causing poor freezing effect, reducing the cell wall puncture rate, reducing the utilization rate of raw materials, and also avoiding the continuous imbalance causing poor hot bath effect, increasing the hot bath cost and energy consumption.

[0100] When the low-temperature deformation sleeve 12 is in the extreme elongation state or the high-temperature deformation sleeve 22 is in the extreme contraction state, and the balanced induction slider 33 continuously triggers the unbalanced eccentric contact block 35, it indicates that the temperature control of the freeze-thaw cycle device is in an abnormal state or an ineffective state of adjustment and compensation at this time, and the temperature control needs to be adjusted and compensated or an abnormal alarm needs to be given to avoid the extraction loss caused by device damage.

[0101] Figure 2 And Figure 4 - Figure 8 The freeze-thaw balance induction unit includes a freeze-thaw temperature control balance trigger module, a freeze-thaw temperature control imbalance abnormal module, a low-temperature lower limit trigger module, a low-temperature upper limit trigger module, a high-temperature lower limit trigger module and a high-temperature upper limit trigger module. The output ends of the freeze-thaw temperature control balance trigger module, the freeze-thaw temperature control imbalance abnormal module, the low-temperature lower limit trigger module, the low-temperature upper limit trigger module, the high-temperature lower limit trigger module and the high-temperature upper limit trigger module are all connected with a freeze-thaw balance induction processing module, and the output end of the freeze-thaw balance induction processing module is signal connected with an intelligent collaborative processing unit;

[0102] The input end of the freeze-thaw temperature control balance trigger module is connected with the balance eccentric contact block 34, the input end of the freeze-thaw temperature control imbalance abnormality module is connected with the balance eccentric contact block 34, the imbalance eccentric contact block 35, the low-temperature upper limit trigger module and the high-temperature lower limit trigger module, the input end of the low-temperature lower limit trigger module is connected with the low-temperature lower limit contact block 15, the input end of the low-temperature upper limit trigger module is connected with the low-temperature upper limit contact block 16, the input end of the high-temperature lower limit trigger module is connected with the high-temperature lower limit contact block 26, the input end of the high-temperature upper limit trigger module is connected with the high-temperature lower limit contact block 26, and the cooperation of the modules in the freeze-thaw balance sensing unit can realize the detection and judgment of the temperature state and the temperature balance state in the freeze-thaw cycle process, simplify the judgment and processing process of the freeze-thaw temperature, reduce the difficulty of data processing of the subsequent intelligent processing end, improve the data processing efficiency, promote the timeliness of temperature compensation regulation, thereby reducing the economic loss caused by temperature abnormalities, and effectively promoting the control accuracy of the freeze-thaw cycle process, realizing the dynamic maintenance of temperature, thereby promoting the extraction rate of chlorella polysaccharide and reducing the energy consumption in the extraction process, realizing the economy of chlorella polysaccharide extraction. Through independent sensing and compensation calculation of the freeze-thaw temperature, the efficiency of temperature regulation and compensation can be further improved, the energy consumption is reduced, the properties of chlorella polysaccharide and the extraction rate of chlorella polysaccharide are ensured, the influence of temperature fluctuation on the extraction rate of chlorella polysaccharide is reduced, and the influence of high temperature on the quality of chlorella polysaccharide is reduced, thereby promoting the temperature regulation accuracy and efficiency of the chlorella polysaccharide extraction process, promoting the production of chlorella polysaccharide and the utilization rate of raw materials, and promoting the economic benefits of chlorella polysaccharide extraction.

[0103] Figure 1 - Figure 8 It is shown that during the operation of the freeze-thaw cycle device, the freeze-thaw temperature is an important indicator affecting the extraction rate of chlorella polysaccharide. Due to the temperature sensitivity of chlorella polysaccharide, it is necessary to not only maintain sufficient temperature difference during the freeze-thaw process, but also reduce the influence of high temperature on chlorella polysaccharide. Therefore, accurate detection and regulation of the temperature in the freeze-thaw cycle process are required. In cooperation with the cold freezing temperature sensing group 1, the hot melting temperature sensing group 2 and the eccentric balance sensing group 3, the actual freeze-thaw temperature and the temperature balance can be effectively detected and dynamically detected in real time. The freeze-thaw temperature balance compensation unit and the freeze-thaw temperature balance regulation unit can effectively realize high-precision regulation and compensation of the freeze-thaw temperature, thereby avoiding the loss of chlorella polysaccharide caused by excessive temperature fluctuation or high-temperature environment, and effectively avoiding the decrease of cell structure piercing rate caused by unbalanced temperature difference.

[0104] When the freezing temperature is within the set range and the temperature is low, the composite temperature deformation strips in the low-temperature deformation sleeve 12 will cause the low-temperature deformation sleeve 12 to produce a certain amount of shrinkage deformation, so that the low-temperature deformation sleeve 12 drives the low-temperature accompanying touch plate 13 to move to the lower position inside the freezing response box 11, and through the low-temperature accompanying touch plate 13, the low-temperature balance elastic sleeve 14 produces a downward elongation effect, releasing the pressure in the low-temperature balance elastic sleeve 14, and under the connection of the balance contraction pipe 32 on the left and the low-temperature balance elastic sleeve 14, the balance response slider 33 can move to the left side; and when the hot bath temperature is within the set range and the temperature is higher, the composite temperature deformation strips in the high-temperature deformation sleeve 22 will cause the high-temperature deformation sleeve 22 to produce a certain amount of elongation deformation, so that the high-temperature deformation sleeve 22 drives the high-temperature accompanying touch plate 23 to move to the upper position inside the hot-melt response box 21, and through the high-temperature accompanying touch plate 23, the high-temperature balance combustible sleeve 24 produces a upward compression effect, increases the pressure in the high-temperature balance combustible sleeve 24, and under the connection of the balance contraction pipe 32 on the right and the high-temperature balance combustible sleeve 24, the balance response slider 33 is squeezed and pushed to move to the left, and the balance eccentric contact block 34 is triggered, the freeze-thaw temperature control balance module in the freeze-thaw balance response unit is triggered, and the freeze-thaw balance effective trigger data is transmitted to the freeze-thaw balance response processing module, and after the freeze-thaw balance response processing module receives the data, it is transmitted to the intelligent collaborative processing unit. The intelligent collaborative processing unit will judge the freeze-thaw data control effect at this time, that is, no control compensation effect is generated, the temperature control effect at this time is maintained, and the basic operation parameters of the freeze-thaw temperature balance control unit are not controlled and compensated.

[0105] When the freezing temperature is at the lower limit of the set range, the composite temperature deformation strips in the low-temperature deformation sleeve 12 will cause the low-temperature deformation sleeve 12 to produce extreme shrinkage deformation, so that the low-temperature deformation sleeve 12 drives the low-temperature accompanying touch plate 13 to move to the abutting position with the low-temperature lower limit touch block 15, and drives the low-temperature balance elastic sleeve 14 to produce a continuous downward elongation effect, maintaining the triggering effect of the balance response slider 33 on the balance eccentric contact block 34. The trigger of the low-temperature lower limit touch block 15 will transmit the signal to the low-temperature lower limit trigger module, and the low-temperature lower limit trigger module will transmit the data to the freeze-thaw balance response processing module. The freeze-thaw balance response processing module will synchronize the low-temperature lower limit trigger data and the freeze-thaw balance continuous trigger data to the intelligent collaborative processing unit, and the intelligent collaborative processing unit will transmit the low-temperature lower limit trigger data, the freeze-thaw balance continuous trigger data, the freezing temperature set range data and the actual temperature data in the freeze-thaw cycle device to the freeze-thaw balance compensation unit. The freeze-thaw balance compensation unit calculates the temperature compensation according to the obtained data, and then transmits the temperature compensation data to the intelligent collaborative processing unit. The intelligent collaborative processing unit controls the freezing temperature in the freeze-thaw cycle device through the freeze-thaw temperature balance control unit, reduces the persistence of the freezing effect, and thus reduces the freezing energy consumption;

[0106] When the hot bath temperature is at the upper limit value of the set range, the composite temperature deformation strip in the high-temperature deformation sleeve 22 drives the high-temperature deformation sleeve 22 to produce a limit elongation effect, so that the high-temperature deformation sleeve 22 drives the high-temperature accompanying touch plate 23 to move to the abutting position with the high-temperature upper limit touch block 25, and drives the high-temperature balance combustible sleeve 24 to produce a continuous upward contraction, maintaining the triggering effect of the balance induction sliding block 33 on the balance eccentric touch block 34 at this time. The triggering of the high-temperature upper limit touch block 25 will transmit a signal to the high-temperature upper limit triggering module, and the high-temperature upper limit triggering module will transmit data to the freeze-thaw balance induction processing module. The freeze-thaw balance induction processing module will synchronize the high-temperature upper limit triggering data and the freeze-thaw balance continuous triggering data to the intelligent collaborative processing unit. The intelligent collaborative processing unit will transmit the high-temperature upper limit triggering data, the freeze-thaw balance continuous triggering data, the hot bath temperature set range data and the actual temperature data in the freeze-thaw cycle device to the freeze-thaw balance compensation unit. The freeze-thaw balance compensation unit calculates the temperature compensation according to the obtained data, and then transmits the temperature compensation data to the intelligent collaborative processing unit. The intelligent collaborative processing unit regulates the hot bath temperature in the freeze-thaw cycle device through the freeze-thaw temperature balance regulation unit, reduces the persistence of high-temperature effect, and thus reduces the influence of high temperature on the production of the small ball algae polysaccharide. The degradation and activity loss of the small ball algae polysaccharide are reduced, and the extraction rate of the small ball algae polysaccharide is ensured.

[0107] When the freezing temperature is within the set range and the temperature is higher, the composite temperature deformation strips in the low-temperature deformation sleeve 12 will drive the low-temperature deformation sleeve 12 to produce a certain amount of elongation deformation, so that the low-temperature deformation sleeve 12 drives the low-temperature accompanying touch plate 13 to move to the upper position inside the freezing response box 11, and through the low-temperature accompanying touch plate 13, the low-temperature balance elastic sleeve 14 produces a compression action upward, increasing the pressure in the low-temperature balance elastic sleeve 14, under the connection action of the balance contraction pipe 32 located on the left side and the low-temperature balance elastic sleeve 14, the balance response slider 33 is extruded to produce a leftward movement trend; and when the hot bath temperature is within the set range and the temperature is lower, the composite temperature deformation strips in the high-temperature deformation sleeve 22 will drive the high-temperature deformation sleeve 22 to produce a certain amount of contraction deformation, so that the high-temperature deformation sleeve 22 drives the high-temperature accompanying touch plate 23 to move to the lower position inside the hot melt response box 21, and through the high-temperature accompanying touch plate 23, the high-temperature balance flammable sleeve 24 produces a downward elongation, releasing the pressure in the high-temperature balance flammable sleeve 24, under the connection action of the balance contraction pipe 32 located on the right side and the high-temperature balance flammable sleeve 24, the balance response slider 33 has a rightward movement allowance, and then under the extrusion of the low-temperature balance elastic sleeve 14, the balance response slider 33 moves to the right, the balance eccentric trigger 34 is disconnected, and the unbalanced eccentric trigger 35 is triggered, the disconnection of the balance eccentric trigger 34 will prompt the freeze-thaw temperature balance module in the freeze-thaw balance response unit to transmit the disconnection signal to the freeze-thaw temperature imbalance abnormal module, the trigger of the unbalanced eccentric trigger 35 transmits the freeze-thaw temperature imbalance data to the freeze-thaw temperature imbalance abnormal module, the freeze-thaw temperature imbalance abnormal module transmits the data to the freeze-thaw balance response processing module, after the freeze-thaw balance response processing module receives the data, it transmits the data to the intelligent collaborative processing unit, the intelligent collaborative processing unit transmits the temperature imbalance data, the hot bath temperature set range data and the actual temperature data in the freeze-thaw cycle device to the freeze-thaw balance compensation unit, the freeze-thaw balance compensation unit calculates the temperature compensation according to the obtained data, and then transmits the temperature compensation data to the intelligent collaborative processing unit, the intelligent collaborative processing unit adjusts and compensates the freeze-thaw temperature in the freeze-thaw cycle device through the freeze-thaw temperature balance adjustment unit, so as to ensure the balance effect of the freeze-thaw temperature, reduce the energy consumption, effectively promote the effectiveness of the freeze-thaw cycle on the destruction of cell structure, reduce the number of freeze-thaw cycles and hot bath time, and promote the efficiency of hot bath.

[0108] At the upper limit of the set range of the freezing temperature, the composite temperature-sensitive deformation strip in the low-temperature deformation sleeve 12 drives the low-temperature deformation sleeve 12 to produce extreme elongation deformation, so that the low-temperature deformation sleeve 12 drives the low-temperature accompanying touch plate 13 to move to the abutting position with the low-temperature upper limit touch block 16, and drives the low-temperature balance elastic sleeve 14 to produce continuous upward contraction, maintaining the triggering effect of the balanced sensing slider 33 on the unbalanced eccentric touch block 35 at this time. The triggering of the low-temperature upper limit touch block 16 will transmit a signal to the low-temperature upper limit triggering module, and the low-temperature upper limit triggering module will transmit data to the freeze-thaw balance sensing processing module and the freeze-thaw temperature control imbalance abnormal module. The freeze-thaw temperature control imbalance abnormal module transmits the data of continuous imbalance to the freeze-thaw balance sensing processing module, and the freeze-thaw balance sensing processing module synchronizes the triggering data of the low-temperature upper limit and the continuous triggering data of the freeze-thaw imbalance to the intelligent collaborative processing unit. The intelligent collaborative processing unit transmits the low-temperature upper limit triggering data, the freeze-thaw imbalance continuous triggering data, the freezing temperature set range data and the actual temperature data in the freeze-thaw cycle device to the freeze-thaw balance compensation unit. The freeze-thaw balance compensation unit calculates the temperature compensation according to the obtained data, and then transmits the temperature compensation data to the intelligent collaborative processing unit. The intelligent collaborative processing unit adjusts the freezing temperature in the freeze-thaw cycle device through the freeze-thaw temperature balance adjustment unit, ensures the effectiveness of the freezing effect, promotes the generation of freezing crystallization, improves the damage effect on the cell structure, and further promotes the release amount of chlorella polysaccharide;

[0109] At the lower limit of the set range of the hot bath temperature, the composite temperature-sensitive deformation strip in the high-temperature deformation sleeve 22 drives the high-temperature deformation sleeve 22 to produce extreme contraction, so that the high-temperature deformation sleeve 22 drives the high-temperature accompanying touch plate 23 to move to the abutting position with the high-temperature lower limit touch block 26, and drives the high-temperature balance elastic sleeve 24 to produce continuous downward elongation, maintaining the triggering effect of the balanced sensing slider 33 on the unbalanced eccentric touch block 35 at this time. The triggering of the high-temperature lower limit touch block 26 will transmit a signal to the high-temperature lower limit triggering module, and the high-temperature lower limit triggering module will transmit data to the freeze-thaw balance sensing processing module and the freeze-thaw temperature control imbalance abnormal module. The freeze-thaw temperature control imbalance abnormal module transmits the data of continuous imbalance to the freeze-thaw balance sensing processing module, and the freeze-thaw balance sensing processing module synchronizes the triggering data of the low-temperature upper limit and the continuous triggering data of the freeze-thaw imbalance to the intelligent collaborative processing unit. The intelligent collaborative processing unit transmits the low-temperature upper limit triggering data, the freeze-thaw imbalance continuous triggering data, the freezing temperature set range data and the actual temperature data in the freeze-thaw cycle device to the freeze-thaw balance compensation unit. The freeze-thaw balance compensation unit calculates the temperature compensation according to the obtained data, and then transmits the temperature compensation data to the intelligent collaborative processing unit. The intelligent collaborative processing unit adjusts the freezing temperature in the freeze-thaw cycle device through the freeze-thaw temperature balance adjustment unit, ensures the effectiveness of the freezing effect, promotes the generation of freezing crystallization, improves the damage effect on the cell structure, and further promotes the release amount of chlorella polysaccharide.

[0110] While the above regulation is carried out, the intelligent collaborative processing unit can verify the effectiveness of the temperature compensation regulation through the real-time data transmitted by the freeze-thaw balance sensing processing module, and if the verification is effective, the regulation at this time is maintained, if the verification is invalid, the temperature compensation regulation is continued, and if the temperature compensation regulation is invalid for three times or more, the abnormal data display and abnormal alarm are generated through the abnormal alarm unit.

[0111] The third embodiment:

[0112] Figure 1 - Figure 4 The application of Chlorella polysaccharide in soothing and oil control products is shown, 75% of water, 3% of Chlorella polysaccharide, 3.5% of 1,2-hexanediol, 1.5% of p-hydroxyacetophenone and 1.5% of 1,2-pentanediol are mixed in mass percentage, the pH is adjusted to 6.0-6.5, and the soothing and oil control product is prepared. Since Chlorella polysaccharide has the characteristics of being easily consumed by good bacteria and being difficult to be utilized by bad bacteria, the application of Chlorella polysaccharide in the soothing and oil control product can realize the dynamic management of skin resident bacteria, and through the microecological skin care effect, the soothing and oil control product can effectively realize the effects of oil control and acne removal, soothing and repair and anti-aging and moisturizing on the skin.

[0113] In combination with the current actual demand, the above-mentioned embodiments adopted by the present application are not limited to the scope, and various changes made within the knowledge range of those skilled in the art without departing from the concept of the present application still fall within the protection scope of the present application.

Claims

1. A method for extracting polysaccharides from Chlorella, characterized in that: It includes an intelligent collaborative system and an extraction production line that works in conjunction with it. The intelligent collaborative system includes an intelligent processing terminal, the input terminal of which is connected to an extraction data acquisition terminal, and the output terminal of which is connected to a collaborative control terminal. Includes the following steps: S1. Set and run basic extraction parameters. S11. The intelligent processing end sets various extraction parameters of the extraction production line through basic extraction parameters; S12. Then, the control parameters of ultrasonic treatment, freeze-thaw cycle and separation and purification process in the extraction line are coordinated and regulated through the coordinated regulation terminal; S2. Ultrasonic treatment, S21. Mix the Chlorella raw material with the extraction solvent according to the solid-liquid ratio; S22. The ultrasonic instrument operates according to the control instructions of the collaborative control terminal to process the mixture. The ultrasonic cavitation effect destroys the cell membrane and cell wall, releasing intracellular components. S23. Simultaneously, the data acquisition terminal collects data on the ultrasonic instrument's operating power, frequency, processing time, and raw material weight, and synchronizes this data to the intelligent processing terminal. S3. Freeze-thaw cycle, S31. The freeze-thaw cycle device operates according to the control instructions of the coordinated control end, and performs a cycle of freezing and thawing on the ultrasonically treated mixture, using ice crystal formation to destroy cell structure and further release intracellular components; S32. Simultaneously, the data acquisition terminal collects data on the freezing temperature, freezing time, thawing temperature, thawing time, and number of cycles of the freeze-thaw cycle device, and synchronizes it to the intelligent processing terminal; S4. Separation and purification, S41. After the number of cycles set at the collaborative control terminal is completed, the centrifuge operates according to the control instructions of the collaborative control terminal and collects the separated liquid; S42. The column chromatography unit operates according to the instructions of the coordinated control end, concentrates the separation liquid and adsorbs Chlorella polysaccharides, and separates neutral Chlorella polysaccharides and acidic Chlorella polysaccharides by elution with salt solutions of different concentrations. S43. The column chromatography apparatus operates according to the instructions of the coordinated control terminal, dialyzing the eluent containing Chlorella polysaccharide to remove salt, thereby obtaining Chlorella polysaccharide; S44. Simultaneously, the data acquisition terminal collects data on the centrifuge's rotation speed, running time, initial separation liquid weight, and separation liquid weight; it also collects data on the column chromatography device's flow rate, salt solution concentration gradient, separation time, dialysis time, and Chlorella polysaccharide weight, and synchronizes these data to the intelligent processing terminal. S5. Intelligent collaborative control, S51. The intelligent processing end analyzes and processes the collected extraction data, analyzes the production capacity and energy consumption data of each stage in the Chlorella polysaccharide extraction process, as well as the overall extraction production capacity and energy consumption data, adjusts and compensates the extraction parameters based on the actual production and consumption balance data, and transmits the adjustment and compensation data to the collaborative control end. S52. The coordinated control end uses the compensated parameters to coordinately control the operation data of ultrasonic treatment, freeze-thaw cycle and separation and purification, respectively, to promote the balance between the production capacity and energy consumption of Chlorella polysaccharide, and repeat step S1. S53. The intelligent processing end can then repeat step S51 based on the collected data transmitted from the data acquisition end to verify the control compensation parameters and make appropriate dynamic adjustments based on production capacity requirements.

2. The method for extracting Chlorella polysaccharides according to claim 1, characterized in that: The data acquisition terminal includes an ultrasonic operation acquisition unit, a freeze-thaw cycle acquisition unit, a centrifugation operation acquisition unit, and a chromatography data acquisition unit; The intelligent processing unit includes an intelligent collaborative processing unit, a collaborative compensation unit, a reverse analysis unit, and a feedback verification processing unit. The intelligent collaborative processing unit is signal-connected to the collaborative compensation unit, the reverse analysis unit, and the feedback verification processing unit, respectively. The coordinated control unit includes an ultrasonic operation control unit, a freeze-thaw cycle control unit, a centrifugation operation control unit, and a chromatography control unit; The output terminals of the ultrasonic operation acquisition unit, freeze-thaw cycle acquisition unit, centrifugation operation acquisition unit, and chromatography data acquisition unit are all connected to the intelligent collaborative processing unit. The input terminals of the ultrasonic operation control unit, freeze-thaw cycle control unit, centrifugation operation control unit, and chromatography control unit are all connected to the intelligent collaborative processing unit.

3. The method for extracting Chlorella polysaccharides according to claim 2, characterized in that: The extraction line includes an ultrasonic instrument, a freeze-thaw circulation device located at the output end of the ultrasonic instrument, a centrifuge located at the output end of the freeze-thaw circulation device, and a column chromatography device located at the output end of the centrifuge. The input terminal of the ultrasonic operation acquisition unit is connected to the ultrasonic instrument signal, the input terminal of the freeze-thaw cycle acquisition unit is connected to the freeze-thaw cycle device signal, the input terminal of the centrifugation operation acquisition unit is connected to the centrifuge signal, and the input terminal of the chromatography data acquisition unit is connected to the column chromatography device signal. The output of the ultrasonic operation control unit is connected to the ultrasonic instrument signal, the output of the freeze-thaw cycle control unit is connected to the freeze-thaw cycle device signal, the output of the centrifugation operation control unit is connected to the centrifuge signal, and the output of the chromatography control unit is connected to the column chromatography device signal.

4. The method for extracting Chlorella polysaccharides according to claim 2, characterized in that: When analyzing the production capacity and energy consumption data at each stage of the Chlorella polysaccharide extraction process in step S51 at the intelligent processing terminal, S511. The data acquisition terminal collects data from each extraction step in the extraction production line. The ultrasonic operation acquisition unit collects data on the ultrasonic instrument's operating power, frequency, processing time, and raw material weight. The freeze-thaw cycle acquisition unit collects data on the freezing temperature, freezing time, thawing temperature, thawing time, and number of cycles of the freeze-thaw cycle device. The centrifugation operation data acquisition unit collects data on centrifuge speed, running time, initial separation liquid weight, and separation liquid weight. The flow rate, salt solution concentration gradient, separation time, dialysis time, and Chlorella polysaccharide weight of the column chromatography apparatus were collected by the chromatography data acquisition unit. S512. The intelligent processing unit processes and analyzes the collected data. The ultrasonic operation acquisition unit, freeze-thaw cycle acquisition unit, centrifugation operation acquisition unit, and chromatography data acquisition unit transmit the acquired data to the intelligent collaborative processing unit; The intelligent collaborative processing unit calculates the extraction energy consumption for ultrasonic treatment, freeze-thaw cycles, and separation and purification based on the acquired data. The intelligent collaborative processing unit calculates the capacity of the centrifuge based on the initial weight data and weight data of the separated liquid, and calculates the capacity of the column chromatography device based on the weight of the separated liquid and the weight of Chlorella polysaccharide. The intelligent collaborative processing unit will also transmit the raw material weight during the ultrasonic treatment process and the separation liquid weight during the separation and purification process to the reverse analysis unit. The reverse analysis unit will perform reverse reasoning on the sum of the production capacity of ultrasonic treatment and freeze-thaw treatment, and then feed back the sum of the production capacity of ultrasonic treatment and freeze-thaw treatment obtained by reverse reasoning to the intelligent collaborative processing unit. The intelligent collaborative processing unit summarizes and calculates the energy production and energy consumption data of ultrasonic treatment and freeze-thaw treatment based on the data it calculates and receives, and obtains the summary energy production and energy consumption balance data of ultrasonic treatment and freeze-thaw treatment. It also calculates the independent energy production and energy consumption balance data of centrifuge and column chromatography device in the separation and purification process, and obtains the centrifugation energy production and energy consumption balance data and column chromatography energy production and energy consumption balance data. S513. The intelligent processing unit adjusts and compensates for the analyzed data. The intelligent collaborative processing unit transmits the aggregated production and consumption balance data, centrifugation production and consumption balance data, column chromatography production and consumption balance data, and basic extraction parameters it calculates to the collaborative compensation unit. The collaborative compensation unit assigns a single collaborative compensation effect to the ultrasonic treatment and freeze-thaw treatment based on the aggregated production and consumption balance data, that is, it only performs parameter adjustment and compensation for any one of the ultrasonic treatment and freeze-thaw treatment processes. The collaborative compensation unit performs synchronous collaborative compensation on the centrifuge and column chromatography apparatus based on the centrifugation production and consumption balance data and the column chromatography production and consumption balance data, that is, it performs synchronous collaborative parameter adjustment and compensation on the centrifuge and column chromatography apparatus. The collaborative compensation unit feeds back the single collaborative compensation data and the synchronous collaborative compensation data to the intelligent collaborative processing unit. After processing and transforming the data, the intelligent collaborative processing unit transmits the extracted parameters after adjustment and compensation to the corresponding ultrasonic operation control unit, freeze-thaw cycle control unit, centrifugation operation control unit and chromatography control unit, respectively.

5. The method for extracting Chlorella polysaccharides according to claim 4, characterized in that: In step S53, when verifying the control compensation parameters and making applicability-based dynamic adjustments according to capacity demand, S531. The intelligent processing unit collects, analyzes, and processes the data after regulation and compensation. The intelligent collaborative processing unit collects data from each process after regulation and compensation through the ultrasonic operation acquisition unit, freeze-thaw cycle acquisition unit, centrifugation operation acquisition unit, and chromatography data acquisition unit. The data is analyzed and processed by repeating steps S511 to S512. Then, the regulation and compensation parameters, basic extraction parameters, and production and consumption balance data of each process are transmitted to the feedback verification processing unit. S532. Verification of control and compensation parameters by the intelligent processing terminal. The feedback verification processing unit verifies whether a single collaborative compensation data point promotes a balance between production and consumption, verifies whether synchronous collaborative compensation data promotes a balance between production and consumption, and verifies whether the overall extraction process promotes a balance between production and consumption. Then, the verification data is fed back and transmitted to the intelligent collaborative processing unit; S533. The intelligent processing unit dynamically adjusts based on the verified data. The intelligent collaborative processing unit processes and transforms the verification data on whether each process generates a direction that promotes the balance between production and consumption, as well as the overall extraction process, and then transmits these data, the control and compensation parameters, and the control and compensation data of each production and consumption balance data to the collaborative compensation unit, repeating step S513.

6. The method for extracting Chlorella polysaccharides according to claim 2, characterized in that: The freeze-thaw cycle device includes a freezing device and a hot bath device. The freezing device is equipped with a freezing temperature sensing group (1), and the hot bath device is equipped with a hot thaw temperature sensing group (2). An eccentric balance sensing group (3) is connected between the freezing temperature sensing group (1) and the hot thaw temperature sensing group (2). The data acquisition terminal also includes a freeze-thaw balance sensing unit. The input terminal of the freeze-thaw balance sensing unit is connected to the signals of the freezing temperature sensing group (1), the heat-thaw temperature sensing group (2), and the eccentric balance sensing group (3), respectively. The output terminal of the freeze-thaw balance sensing unit is connected to the signal of the intelligent collaborative processing unit. The intelligent processing end also includes a freeze-thaw balance compensation unit, which is signal-connected to the intelligent collaborative processing unit. The collaborative control end includes a freeze-thaw temperature balance control unit, whose input is signal-connected to the intelligent collaborative processing unit, and whose output is signal-connected to the refrigeration device and the heating device, respectively.

7. The method for extracting Chlorella polysaccharides according to claim 6, characterized in that: The freezing temperature sensing group (1) includes a freezing sensing box (11) fixedly installed in the freezing device. A low temperature deformation sleeve (12) is fixedly connected to the lower inner wall of the freezing sensing box (11). A low temperature accompanying contact plate (13) that slides with the inside of the freezing sensing box (11) is fixedly connected to the upper end of the low temperature deformation sleeve (12). A low temperature balancing elastic sleeve (14) is fixedly connected to the upper end of the low temperature balancing elastic sleeve (14), and the upper end of the low temperature balancing elastic sleeve (14) is fixedly connected to the upper inner wall of the freezing sensing box (11). A low temperature lower limit contact block (15) located below the low temperature accompanying contact plate (13) is fixedly connected to both the left and right inner walls of the freezing sensing box (11). A low temperature upper limit contact block (16) located above the low temperature accompanying contact plate (13) is fixedly connected to both the left and right inner walls of the freezing sensing box (11). The heat-sensing assembly (2) includes a heat-sensing box (21) fixedly installed in the heat bath device. A high-temperature deformation sleeve (22) is fixedly connected to the lower inner wall of the heat-sensing box (21). A high-temperature accompanying contact plate (23) that slides with the inner wall of the heat-sensing box (21) is fixedly connected to the upper end of the high-temperature deformation sleeve (22). A high-temperature balancing flammability sleeve (24) is fixedly connected to the upper end of the high-temperature balancing flammability sleeve (24), and the upper end of the high-temperature balancing flammability sleeve (24) is fixedly connected to the upper inner wall of the heat-sensing box (21). A high-temperature upper limit contact block (25) located on the upper side of the high-temperature accompanying contact plate (23) is fixedly connected to the left and right inner walls of the heat-sensing box (21). A high-temperature lower limit contact block (26) located on the lower side of the high-temperature accompanying contact plate (23) is fixedly connected to the left and right inner walls of the heat-sensing box (21). Multiple composite temperature-sensitive deformation strips are fixedly connected between the upper and lower inner walls of the low-temperature deformation sleeve (12) and between the upper and lower inner walls of the high-temperature deformation sleeve (22). The composite temperature-sensitive deformation strips located in the low-temperature deformation sleeve (12) can generate corresponding deformation under low-temperature triggering conditions, and the composite temperature-sensitive deformation strips located in the high-temperature deformation sleeve (22) can generate corresponding deformation under high-temperature triggering conditions. The eccentric balance sensing group (3) includes an eccentric balance sleeve (31) fixedly installed on the outside of the freezing device and the heating device. The left and right ends of the eccentric balance sleeve (31) are fixedly connected to balance contraction tubes (32) that communicate with it. The lower end of the balance contraction tube (32) on the left extends into the freezing sensing box (11) and communicates with the low temperature balance elastic sleeve (14). The lower end of the balance contraction tube (32) on the right extends into the heat melting sensing box (21) and communicates with the high temperature balance elastic sleeve (24). The left end of the inner wall of the eccentric balance sleeve (31) is fixedly connected to a balance eccentric contact block (34), and the right end of the inner wall of the eccentric balance sleeve (31) is fixedly connected to an imbalance eccentric contact block (35). A balance sensing slider (33) is slidably arranged inside the eccentric balance sleeve (31) between the balance eccentric contact block (34) and the imbalance eccentric contact block (35).

8. The method for extracting Chlorella polysaccharides according to claim 7, characterized in that: The freeze-thaw balance sensing unit includes a freeze-thaw temperature control balance trigger module, a freeze-thaw temperature control imbalance abnormality module, a low temperature lower limit trigger module, a low temperature upper limit trigger module, a high temperature lower limit trigger module, and a high temperature upper limit trigger module. The output terminals of the freeze-thaw temperature control balance trigger module, the freeze-thaw temperature control imbalance abnormality module, the low temperature lower limit trigger module, the low temperature upper limit trigger module, the high temperature lower limit trigger module, and the high temperature upper limit trigger module are all connected to a freeze-thaw balance sensing processing module. The output terminal of the freeze-thaw balance sensing processing module is connected to the intelligent collaborative processing unit. The input terminal of the freeze-thaw temperature control balance trigger module is connected to the balance eccentric contact (34) for signal connection. The input terminal of the freeze-thaw temperature control imbalance abnormality module is connected to the balance eccentric contact (34), the imbalance eccentric contact (35), the low temperature upper limit trigger module and the high temperature lower limit trigger module for signal connection respectively. The input terminal of the low temperature lower limit trigger module is connected to the low temperature lower limit contact (15) for signal connection. The input terminal of the low temperature upper limit trigger module is connected to the low temperature upper limit contact (16) for signal connection. The input terminal of the high temperature lower limit trigger module is connected to the high temperature lower limit contact (26) for signal connection. The input terminal of the high temperature upper limit trigger module is connected to the high temperature lower limit contact (26) for signal connection.

9. The method for extracting Chlorella polysaccharides according to claim 2, characterized in that: The data acquisition terminal also includes a parameter command acquisition unit, and the collaborative control terminal also includes a collaborative data display unit and an anomaly warning unit. The input terminal of the parameter command acquisition unit is connected to the signal of the extraction production line control platform. The output terminals of the collaborative data display unit and the anomaly warning unit are both connected to the signal of the extraction production line control platform, and the output terminal of the anomaly display unit is also connected to the alarm signal set on the extraction production line control platform.

10. The application of a Chlorella polysaccharide in a soothing and oil-controlling product, based on a Chlorella polysaccharide prepared by any one of claims 1-9, characterized in that: A soothing and oil-controlling product was prepared by mixing 75% water, 3% Chlorella polysaccharide, 3.5% 1,2-hexanediol, 1.5% p-hydroxyacetophenone, and 1.5% 1,2-pentanediol in the indicated mass percentages and adjusting the pH to 6.0–6.5.

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