Compound essential oil for increasing compactness and elasticity of skin as well as preparation method and application of compound essential oil

Abstract

The invention relates to the field of cosmetics, in particular to compound essential oil for improving skin compactness and elasticity as well as a preparation method and application of the compound essential oil. The compound essential oil comprises green coffee seed oil, camellia seed oil, peony seed oil, cactus seed oil, feather cockscomb seed oil, European plum seed oil and the like, and the compound essential oil can effectively improve skin elasticity and compact effects. Meanwhile, by adopting butanediol, oleamide MIPA disodium sulfosuccinate and ceteareth-60 myristyl glycol, the stability of the compound essential oil in a liquid preparation can be effectively improved, and the liquid preparation is endowed with an excellent and stable effect.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a compound essential oil for increasing skin firmness and elasticity and a preparation method and application thereof. BACKGROUND

[0002] The relationship between essential oils and the increase of skin firmness and elasticity is derived from their multiple regulatory effects on skin structure and cell function. The active ingredients in essential oils can stimulate fibroblast proliferation and promote the synthesis of collagen and elastin. Monoterpenes in essential oils can activate cell signaling pathways and enhance the metabolic activity of fibroblasts, providing "raw material supply" for the skin support structure. Essential oils can inhibit the activity of matrix-degrading enzymes, and skin laxity is closely related to the overactivity of matrix metalloproteinases and elastases, which can destroy collagen and elastin. Components such as citronellol and linalool in essential oils can inhibit elastase activity and reduce elastin degradation, maintaining the integrity of the elastin network. Essential oils can also improve skin microcirculation. Components such as limonene in essential oils can promote dermal blood circulation, increase the delivery of oxygen and nutrients, and provide a more adequate metabolic environment for fibroblasts, indirectly supporting the synthesis and repair of extracellular matrix. In addition, essential oils can also have anti-inflammatory and antioxidant effects. Chronic inflammation caused by factors such as ultraviolet light and pollution can accelerate skin aging and lead to laxity. Phenolic components such as terpinen-4-ol in essential oils have anti-inflammatory properties and can reduce the damage of inflammatory factors to the dermis. At the same time, polyphenols and flavonoids in essential oils can scavenge free radicals and delay fibroblast aging.

[0003] Invention patent CN110090178B discloses a compound essential oil for skin tightening and antioxidant, which comprises the following raw materials in mass percentage: wheat germ oil 50-80%, strawberry seed oil 5-15%, selaginella uncinata extract 1.5-2.5%, ethylhexyl palmitate 6-12%, farnesol 0.5-1%; preferably, it also comprises 5-15% aloe vera oil and 3-5% of myrtle leaf oil.

[0004] Invention patent CN119950367B discloses a natural firming and anti-wrinkle skin lightening plant essential oil, a preparation method and application thereof. The essential oil components include frankincense oil, sandalwood oil, lavender oil, myrrh oil, Italian wax chrysanthemum flower oil, rockrose oil, rose oil, rockrose root oil, and marine sterol. Marine sterol is a mixture of syltasterol and gorgonian sterol. The plant essential oil can be applied in skin care products such as water, mask, serum, emulsion, cream, and foundation.

[0005] The application patent CN117618293B discloses a kind of peptide essential oil for anti-wrinkle firming, which includes the following components by mass fraction: 0.1-0.5 parts of rose oil, 0.5-1 parts of citrus medica limonum peel oil, 0.05-0.5 parts of jasmine flower oil, 0.1-0.5 parts of water-in-oil composite peptide, 90-99 parts of base oil.

[0006] The application patent CN118207043B discloses a rosemary essential oil composition for slimming and firming skin. The preparation of the composition includes: selecting dry rosemary, pelargonium, lemon, lotus leaf and marjoram, respectively crushing to obtain powder; mixing the powders according to the amount to obtain mixed powder, then adding aqueous ethanol solution, heating and stirring; cooling, filtering, concentrating the filtrate to obtain concentrate A; adding an extractant composed of aqueous phase and organic phase of organic solvent, heating and extracting, removing the aqueous phase, and removing the organic solvent from the organic phase to obtain the composition.

[0007] The application patent CN107929101B discloses a firming massage essential oil and its preparation method. The firming massage essential oil is prepared from the following raw materials: jojoba extract, PEG-40 hydrogenated castor oil, menthol, menthol lactate, propylene glycol, camphor, borneol, rosemary leaf oil, pigment, sweet orange oil, PPG-1-PEG-9 lauryl glycol ether, fatty alcohol polyether-7, vanillyl butyl ether, iopropynyl butylcarbamate, methyl isothiazolinone, ginseng seed oil.

[0008] There are relatively few technologies reported in the prior art for preparing green coffee seed oil, camellia seed oil, peony seed oil and cactus seed oil by cold pressing method, and then formulating the four together to prepare a compound essential oil for increasing skin firmness and elasticity. Therefore, it is necessary to provide related research technologies to fill the gap in this field, and it is also necessary to solve the technical problem of how to improve the stability of this type of compound essential oil. SUMMARY

[0009] To solve the above technical problems, the present application provides a compound essential oil for increasing skin firmness and elasticity and a preparation method thereof.

[0010] The present application uses green coffee seed oil, camellia seed oil, peony seed oil and cactus seed oil as raw materials to optimize the compound essential oil for increasing skin firmness and elasticity.

[0011] The present application uses cold pressing method (conditions below 40℃ or 30℃) to prepare green coffee seed oil, camellia seed oil, peony seed oil and cactus seed oil to effectively retain the active ingredients of essential oil.

[0012] The cold pressing method includes the following steps: the raw material (green coffee seed, camellia seed, peony seed or cactus seed) is extruded to release the essential oil mixture, then the essential oil mixture is filtered, and then the upper layer material is obtained by centrifugation, filtration and filtration of the filtrate.

[0013] The method for preparing the green coffee seed essential oil, the camellia seed essential oil, the peony seed essential oil or the cactus seed essential oil includes the following steps: the raw material (green coffee seed, camellia seed, peony seed or cactus seed) is taken, the pressure is increased to 20-35 MPa, the essential oil mixture is extruded, the essential oil mixture is filtered by using 20-300 mesh gauze, the filter is obtained, then the filter is centrifuged at 2000-10000 rpm, the upper layer oily material is taken after centrifugation and filtered by using a filter membrane with a pore size of less than 10 microns, and the filtrate obtained by filtration is the essential oil. The temperature of the whole process is lower than 40 DEG C or 30 DEG C, including the processes of increasing the pressure, extruding the essential oil mixture, gauze filtering, centrifugation, filter membrane filtering and the like.

[0014] The green coffee seed, the camellia seed, the peony seed or the cactus seed as the raw material can be optionally subjected to the processes of cleaning, draining, low-temperature drying (for example, drying at a temperature lower than 40 DEG C) and / or crushing.

[0015] The compound essential oil for increasing skin tightness and elasticity includes, in terms of weight parts, 15-35 parts of green coffee seed oil, 40-60 parts of camellia seed oil, 8-15 parts of peony seed oil and 15-35 parts of cactus seed oil.

[0016] The compound essential oil for increasing skin tightness and elasticity includes, in terms of weight parts, 20 parts of green coffee seed oil, 50 parts of camellia seed oil, 10 parts of peony seed oil and 20 parts of cactus seed oil.

[0017] The compound essential oil for increasing skin tightness and elasticity further includes, in terms of weight parts, 15-35 parts of celosia argentea seed oil and 10-20 parts of prunus domestica seed oil.

[0018] The preparation method of the celosia argentea seed oil includes heating reflux extraction by using ethyl acetate as a solvent.

[0019] The preparation method of the prunus domestica seed oil includes heating stirring extraction by using a solvent composed of n-hexane and n-heptane at a weight ratio of 1:1-4.

[0020] The preparation method of the celosia argentea seed oil includes the following steps: the celosia argentea seed is crushed, ethyl acetate is added, heating reflux extraction is performed for 2-10 hours, the filtrate is obtained by filtration, and the celosia argentea seed essential oil is obtained by removing ethyl acetate by concentration.

[0021] The preparation method of the above-mentioned European plum seed oil comprises the following steps: crushing European plum seeds, adding a mixed solvent composed of n-hexane and n-heptane at a weight ratio of 1:1-4, heating and extracting at 40-55 DEG C for 3-10 hours, filtering to obtain a filtrate, and removing n-hexane and n-heptane by concentration to obtain the European plum seed essential oil.

[0022] In the above-mentioned compound essential oil, the weight ratio of green coffee seed oil and cornflower seed oil is 1:0.5-1.5; the above-mentioned ratio makes the active ingredient combination optimal, and the compound essential oil has better effects of increasing skin elasticity and tightening skin.

[0023] The application further provides a preparation method of the compound essential oil, which comprises the following steps: adding camellia seed oil into a container, starting stirring, adding the remaining raw materials, continuing stirring, filtering, and standing for aging to obtain the compound essential oil.

[0024] The application further provides application of the compound essential oil in preparation of a liquid preparation for increasing skin elasticity and tightness.

[0025] The above-mentioned liquid preparation further comprises 2-10% of butanediol, 2-4% of oleamide MIPA sulfosuccinate disodium, 2-4% of ceteareth-60 myristyl glycol, and the rest of water.

[0026] The application has the following beneficial effects:

[0027] The compound essential oil prepared from the green coffee seed oil, camellia seed oil, peony seed oil, cactus seed oil, cornflower seed oil and European plum seed oil has the effects of effectively increasing skin elasticity and tightening skin.

[0028] The green coffee seed oil and cornflower seed oil of the application can effectively improve the migration rate of fibroblasts and have the effects of effectively improving skin elasticity and tightness.

[0029] The compound essential oil prepared by the application can effectively improve the migration rate of fibroblasts and has the effects of effectively improving skin elasticity and tightness.

[0030] This invention selects a combination of disodium oleamide MIPA sulfosuccinate and cetearyl alcohol polyether-60 myristyl glycol, which is more effective than other emulsifiers in stabilizing compound essential oils, resulting in liquid formulations with higher stability. The combination of disodium oleamide MIPA sulfosuccinate and cetearyl alcohol polyether-60 myristyl glycol in this invention exhibits a synergistic effect in stabilizing compound essential oils and achieving stable formulations. Detailed Implementation

[0031] The advantages and beneficial effects of the technology described in this application are presented in conjunction with specific embodiments.

[0032] I. Preparation of Essential Oils

[0033] Green coffee seed oil: Unripe green coffee beans (variety: Typica) free from mold are washed, drained, and vacuum-dried at 37°C until the water content is 5.7 wt%. The beans are then crushed into small particles to obtain the raw material. The raw material is then placed in a pressurizing device, slowly pressurized to 25 MPa and held for 5 minutes. This process is repeated 3 times. The extracted essential oil mixture is collected and then filtered through 100-mesh gauze to obtain the filter material. The filter material is centrifuged at 6000 rpm for 20 minutes, and the upper oily component is collected. This oil is then filtered through a 0.22 μm pore size filter membrane to obtain the green coffee seed oil. The above process is carried out at room temperature.

[0034] Camellia seed oil: Mature camellia seeds (variety: small-fruited camellia) are vacuum dried at 37℃ to a water content of 6.5wt%, then crushed into small particles to obtain the raw material. The raw material is then placed in a pressurizing device, slowly pressurized to 23MPa and held for 4 minutes, repeated 3 times. The extracted essential oil mixture is collected, filtered through 100-mesh gauze, and centrifuged at 6500rpm for 15 minutes. The upper oily component is collected and filtered through a 0.22μm pore size filter membrane to obtain the camellia seed oil. All the above processes are carried out at room temperature.

[0035] Peony seed oil: Mature peony seeds (variety: Fengdan peony) are vacuum dried at 37℃ to a water content of 4.9wt%, then crushed into small particles to obtain raw materials. These are then placed in a pressurizing device, slowly pressurized to 25MPa and held for 4 minutes, repeated 3 times. The extracted essential oil mixture is collected, filtered through 100-mesh gauze, and centrifuged at 5800rpm for 22 minutes. The upper oily component is collected and filtered through a 0.22μm pore size filter membrane to obtain the peony seed oil. All the above processes are carried out at room temperature.

[0036] Cactus seed oil: Take the mature fruit of Opuntia ficus-indica (variety: Mibonta), wash, drain, and dry at 37°C under vacuum until the water content is 6.1 wt%, crush, and obtain the raw material. Then, place it in a pressurized device, slowly increase the pressure to 20 MPa and maintain the pressure for 6 minutes, repeat the operation 3 times, collect the squeezed essential oil mixture, then filter it with 100 mesh gauze, centrifuge the filtrate at 6000 rpm for 20 minutes, take the upper oily component, and then filter it with a 0.22 μm pore size filter to obtain the cactus seed oil; the above process is carried out at room temperature.

[0037] Celosia argentea seed oil: Take clean celosia argentea (CELOSIA ARGENTEA) seed powder, add ethyl acetate according to a solid-liquid weight ratio of 1:15, heat and reflux extract at about 78°C for 3 hours, cool to 40°C and filter with a 0.22 μm pore size filter to obtain the filtrate, then concentrate under reduced pressure at 58°C to remove ethyl acetate (stop concentrating when ethyl acetate is not detected), and obtain the celosia argentea seed essential oil.

[0038] Prunus domestica seed oil: Take clean prunus domestica (PRUNUS DOMESTICA) seed powder, add a mixed solvent (consisting of n-hexane and n-heptane in a weight ratio of 1:1-4) according to a weight ratio of 1:15, heat and stir at 45°C for 4 hours, filter with a 0.22 μm pore size filter to obtain the filtrate, and concentrate under reduced pressure at 30°C to remove n-hexane and n-heptane (stop concentrating when n-hexane and n-heptane are not detected), and obtain the celosia argentea seed essential oil.

[0039] II. Essential oil collocation effect test

[0040] The green coffee seed oil and the celosia argentea seed oil are collocated in the weight ratios in Table 1.

[0041] Table 1: Essential oil collocation

[0042]

[0043] The products with serial numbers 1-9 in Table 1 above are tested for performance to study the collocation effect of the two.

[0044] Fibroblasts are the main cell type in the dermis, and their core function is to synthesize and secrete extracellular matrix (ECM) components such as collagen, elastin, glycosaminoglycans (such as hyaluronic acid), which together form the "support framework" of the skin and directly determine the tightness, elasticity and fullness of the skin. When fibroblasts are active, ECM is continuously updated, and the skin structure is tight and firm. If the activity of fibroblasts decreases (such as aging, ultraviolet damage), the synthesis of ECM decreases and the degradation increases, and the skin support force decreases, showing loose and wrinkled. The present application uses the test product to characterize the effect of the product on tightening the skin and increasing the elasticity of the skin.

[0045] The test procedure is as follows:

[0046] Consumables: NIH-3T3 mouse fibroblasts; culture medium-Gibco 11965084 DMEM medium; PBS.

[0047] Experimental method: Take the logarithmic growth period of NIH-3T3 mouse fibroblasts according to the density of 8x10 8 4 / well, add culture medium to the well, and incubate at 37℃, 5% CO2 constant temperature until the well is covered, then aspirate the culture medium, add 200μL gun head scratch, and wash the cell fragments with PBS 3 times. Then group administration: the experimental group (sample groups with serial numbers 1-9, named group 1-group 9 in turn) is cultured with culture medium added with 0.15μg / mL test sample (samples with serial numbers 1-9, add sample to a concentration of 0.15μg / mL), while the blank group continues to be cultured with culture medium; the culture conditions of each group: 37℃, 5% CO2 constant temperature culture for 24 hours; each group in parallel 4 times. Calculate the cell migration rate of the blank group and the sample groups with serial numbers 1-9 at different times (12 hours and 24 hours), wherein the calculation method of the cell migration rate is: cell migration rate = 100% x (initial scratch width-culture N hours scratch width) / initial scratch width, and calculate the increase ratio A value of the migration rate of each group at different times compared with the blank group (for example, 12 hours corresponds to A 12 , and 24 hours corresponds to A 24 ). The results are shown in Tables 2 and 3.

[0048] Table 2: Fibroblast migration rate

[0049]

[0050]

[0051] Table 3: Increase ratio A value

[0052] No. A12 A24 Group 1 79.75％ 23.64％ Group 2 84.42％ 24.90％ Group 3 102.29％ 29.72％ Group 4 115.74％ 33.53％ Group 5 125.83％ 36.15％ Group 6 120.18％ 34.61％ Group 7 103.17％ 29.98％ Group 8 93.66％ 27.34％ Group 9 86.68％ 25.16％

[0053] According to the test results of Tables 2-3, the green coffee seed oil and the celosia argentea seed oil of the present application can effectively improve the migration rate of fibroblasts, and have a good effect of improving skin tightness and elasticity. According to the comparison of group 1, 9 and other groups, it can be known that when the total amount of essential oil is maintained, the green coffee seed oil and the celosia argentea seed oil present a synergistic effect of improving the migration rate of fibroblasts within the weight ratio range of 1:0.2-8, and when the ratio is 1:0.05, the effect is added. Especially when the weight ratio of green coffee seed oil and celosia argentea seed oil is 1:0.5-1.5, the effect of improving the migration rate of fibroblasts is optimal.

[0054] III. Effect test of compound essential oil

[0055] The compound essential oil is prepared according to the composition in Table 4, and the raw material usage in Table 4 is by weight.

[0056] Table 4: Composition of compound essential oil

[0057] No. Compound 1 Compound 2 Compound 3 Green coffee seed oil 20 17 24 Camellia seed oil 50 53 50 Peony seed oil 10 12 13 Cactus seed oil 20 18 19 Celosia seed oil 20 24 16 Prunus domestica seed oil 15 17 16

[0058] The preparation method of the compound essential oil is as follows: at room temperature, the camellia seed oil is added into a container, stirring is started at 100 rpm, the remaining raw materials are added, stirring is performed for 30 minutes, a filter membrane with a pore size of 0.22 μm is used for filtration, and the filtered liquid is placed for aging for 1 hour to obtain the compound essential oil.

[0059] The effect of the compound essential oil on the migration rate of fibroblasts is tested.

[0060] The test process is as follows:

[0061] Consumables: NIH-3T3 mouse fibroblasts; culture medium: Gibco 11965084 DMEM culture medium; PBS.

[0062] Experimental method: logarithmic growth phase NIH-3T3 mouse fibroblasts are inoculated in a 6-well plate at a density of 8x10 8 Each group is given drug treatment: the experimental groups (compound essential oils 1 to 3) are cultured in the culture medium added with 0.22 μg / mL test sample (compound 1-3 products, added to a total concentration of 0.22 μg / mL), while the blank group is continuously cultured in the culture medium; the culture conditions of each group are 37℃, 5% CO2 constant temperature culture for 24 hours; each group is in parallel for 4 times. The cell migration rate of the blank group and the sample groups 1-9 at 12 hours is calculated, wherein the calculation method of the cell migration rate is: cell migration rate = 100% x (initial scratch width-cultured for 12 hours scratch width) / initial scratch width, and the increase ratio B24 value of the migration rate of each group compared with the blank group at 24 hours of culture is calculated. The results are shown in Table 5.

[0063] Table 5: Fibroblast migration rate and B24 value

[0064]

[0065]

[0066] According to the test results in Table 5, the compound essential oil prepared by the application can effectively improve the migration rate of fibroblasts, and can effectively improve the skin tightening and elasticity effect.

[0067] Four, application

[0068] The liquid preparation was prepared according to the raw materials and amounts described in Table 6, and the amounts were measured in percentage by weight.

[0069] Table 6: Composition of liquid preparation

[0070]

[0071]

[0072] The preparation method of the above liquid preparation is as follows: 1,3-butanediol and compound essential oil are stirred at room temperature at a stirring speed of 100 rpm for 20 minutes to obtain an oil phase; water and the remaining raw materials are stirred at room temperature at a stirring speed of 100 rpm for 15 minutes to obtain an aqueous phase; the stirring speed is opened to 500 rpm, the oil phase is added to the aqueous phase, and emulsification is performed by stirring for 30 minutes to obtain the liquid preparation.

[0073] The zeta potential of the test sample was tested to characterize the stability of the product. The specific method is as follows: after the above liquid preparation and deionized water are stirred at room temperature at a stirring speed of 500 rpm for 30 minutes at a weight ratio of 1:50, the zeta potential λ1 of the sample is tested by using a Zeta potential tester (NanoBrook90Plus PALS); then after the tested sample is sealed and kept away from light at 40℃ for 3 days, the zeta potential λ2 is tested again. The results are shown in Table 7. The greater the absolute value of λ, the better the stability of the sample.

[0074] Table 7: Zeta potential

[0075] No. λ1 (mV) λ2 (mV) Formulation 1 -39.1 -35.3 Formulation 2 -38.3 -34.4 Formulation 3 -34.7 -30.7 Formulation 4 -33.5 -29.2 Formulation 5 -32.9 -28.7 Formulation 6 -31.8 -27.0

[0076] According to the test results in Table 7, it can be known that the combination of butanediol, oleamide MIPA sulfosuccinate disodium and ceteareth-60 myristyl glycol adopted in the present application has excellent effect on stabilizing compound essential oil and has good stability. It can be known from the comparison of preparations 1-2 and 3-4 that the combination of oleamide MIPA sulfosuccinate disodium and ceteareth-60 myristyl glycol is more conducive to stabilizing compound essential oil than other emulsifiers, and the prepared preparation has higher stability. It can be known from the comparison of preparations 1 and 5-6 that the combination of oleamide MIPA sulfosuccinate disodium and ceteareth-60 myristyl glycol has a synergistic relationship in stabilizing compound essential oil to obtain stable preparation.

[0077] The above specific embodiments are only for illustrating the technical solutions and characteristics of the present application, and the purpose is to make it easier for those skilled in the art to implement, and it cannot be used to limit the protection scope of the present application, and equivalent changes or replacements made according to the spirit and essence of the present application are within the protection scope of the present application.

Claims

1. A compound essential oil for increasing skin firmness and elasticity, characterized in that, The raw material composition comprises, by weight, 15-35 parts of green coffee seed oil, 40-60 parts of camellia seed oil, 8-15 parts of peony seed oil and 15-35 parts of cactus seed oil.

2. The compound essential oil according to claim 1, characterized in that, The raw material composition comprises, by weight, 20 parts of green coffee seed oil, 50 parts of camellia seed oil, 10 parts of peony seed oil and 20 parts of cactus seed oil.

3. The compound essential oil according to claim 1, characterized in that, The green coffee seed oil, the camellia seed oil, the peony seed oil and the cactus seed oil are prepared by cold pressing method, and the temperature of the cold pressing method is lower than 30℃.

4. The complex essential oil according to claim 3, characterized in that, The steps of the cold pressing method comprise: extruding the raw materials to release the essential oil mixture, coarsely filtering, then taking the upper layer material by centrifugation, filtering, and taking the filtrate.

5. The compound essential oil according to claim 1, characterized in that, The compound essential oil further comprises, by weight, 15-35 parts of celosia argentea seed oil and 10-20 parts of prunus domestica seed oil.

6. The complex essential oil according to claim 5, characterized in that, The preparation method of the celosia argentea seed oil comprises heating reflux extraction with ethyl acetate as the solvent; or, The preparation method of the prunus domestica seed oil comprises heating stirring extraction with a solvent composed of n-hexane and n-heptane in a weight ratio of 1:1-4.

7. The compound essential oil according to claim 5, characterized in that, The weight ratio of the green coffee seed oil and the celosia argentea seed oil is 1:0.5-1.

5.

8. A method of preparing the compound essential oil according to any one of claims 1 to 7, characterized in that, The method comprises adding the camellia seed oil into a container, starting stirring, adding the remaining raw materials, continuing stirring, filtering, and standing for aging to obtain the compound essential oil.

9. Use of a complex essential oil according to any one of claims 1 to 7 for the preparation of a liquid preparation for increasing skin firmness and elasticity, characterized in that, The weight percentage content of the compound essential oil in the liquid preparation is 1-15%.

10. Use according to claim 9, characterized in that, The liquid preparation further comprises 2-10% butylene glycol, 2-4% oleamide MIPA sulfosuccinate disodium, 2-4% ceteareth-60 myristyl glycol and the balance of water.

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