Acne removal composition and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to an acne-removing composition and a preparation method thereof. The composition comprises a polygonum cuspidatum extract and a mangnolia officinalis extract, the preparation method of the mangnolia officinalis extract comprises the following steps: mixing mangnolia officinalis with water, heating and sterilizing, cooling, inoculating a mould strain, fermenting, then adding a first solvent, extracting, filtering to obtain a first extracting solution, and concentrating and drying the first extracting solution to obtain the mangnolia officinalis extract; the preparation method of the rhizoma polygoni cuspidati extract comprises the following steps: mixing rhizoma polygoni cuspidati with water, heating, sterilizing, cooling, inoculating yeast strains, fermenting, then adding a second solvent, extracting, filtering to obtain a second extracting solution, and concentrating and drying the second extracting solution to obtain the rhizoma polygoni cuspidati extract. The acne-removing composition prepared by the invention not only can inhibit bacteria, but also can control oil and whiten skin, so that the acne problem is solved from multiple angles.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of acne-removing products, in particular to an acne-removing composition and a preparation method thereof. BACKGROUND

[0002] Acne, also known as "dark sore" or "pimple", is a kind of skin disease caused by blockage and inflammation of hair follicles and sebaceous glands. The causes include the following aspects:

[0003] (1) Increase of male hormones, male hormones affect the secretion of sebum, and increase of male hormones will lead to increased sebum secretion, and excessive sebum secretion on the face is one of the important causes of acne;

[0004] (2) Abnormal keratinization of hair follicle sebaceous glands, in the abnormal keratinization of hair follicle sebaceous glands, the metabolic cycle of keratinocytes is shortened and the keratin formed by the keratinocytes is tightly packed in the hair follicle duct orifice, which leads to occlusion of the orifice, and the mixture of the exfoliated epithelial cells and facial sebum will further block the hair follicle, thus forming a pimple;

[0005] (3) Microbial abnormalities, abnormal proliferation of Propionibacterium acnes is also one of the important factors for the occurrence of acne, accumulation of sebum in sebaceous glands and occlusion of pores form an anaerobic environment, and Propionibacterium acnes is an anaerobic bacteria that thrives on sebum, which will release more bioactive enzymes to decompose triglycerides in sebum into free fatty acids, providing nutrients for the growth and proliferation of Propionibacterium acnes, leading to a vicious cycle;

[0006] (4) Occurrence of local inflammation and immune response, Propionibacterium acnes will cause rupture of the pimple wall, and bacterial keratin will penetrate into the dermis, causing inflammatory mediators in the dermis, and inflammatory cells will gather in the hair follicle duct to form a pustule, in addition, free fatty acids produced by the decomposition of sebum by Propionibacterium acnes enzymes also cause inflammatory reactions, which are manifested as skin redness, cysts and nodules and other symptoms.

[0007] The initial, intermediate and late stages of acne will have different symptoms, causing different degrees of damage to the skin, such as redness, pigmentation, acne marks and acne pits.

[0008] Acne not only affects the appearance of the skin, but if not squeezed properly, it can cause damage to the surrounding tissue, causing more inflammation, delayed healing, and noticeable acne marks. There are many existing anti-acne methods, such as laser treatment, which can quickly destroy P. acnes and effectively inhibit bacterial reproduction, eliminating the stimulating and damaging effects on tissue. However, laser treatment is too strong and cannot fundamentally eliminate acne. Another method is acid therapy, which has a certain inhibitory effect on acne, but fruit acid is highly irritating and has strong side effects. Antibiotic treatment, although effective, can easily lead to drug resistance and long-term use can result in dependent dermatitis.

[0009] Current acne-removing products on the market mainly rely on chemical ingredients such as benzoyl peroxide and salicylic acid to achieve antibacterial and exfoliating effects. They often overlook the overall health and long-term care of the skin, focusing only on short-term effects, which can lead to dry, sensitive skin, and even damage the skin barrier. In addition, these products may not effectively control oil secretion, leading to recurring acne. At the same time, they also lack whitening effects and cannot improve the pigmentation problems caused by acne. For the youth population, the irritability and side effects of these products are particularly noteworthy, and most acne-removing products are single-dimensional and cannot solve the problems of P. acnes colonies, inflammation, redness, pigmentation, and acne marks in multiple dimensions.

[0010] Therefore, there is an urgent need to provide an acne-removing composition that can solve the problems of P. acnes colonies, inflammation, redness, pigmentation, and acne marks in multiple dimensions. SUMMARY

[0011] In view of the above-mentioned shortcomings of the prior art, the purpose of the present application is to provide an acne-removing composition and a preparation method thereof, which can solve the problem of not being able to solve the problems of P. acnes colonies, inflammation, pigmentation, and acne marks in multiple dimensions in the prior art.

[0012] To achieve the above-mentioned purposes and other related purposes, the present application is achieved by the following technical solutions.

[0013] The first aspect of the present application provides an acne-removing composition, which comprises a giant knotweed extract and a magnolia officinalis extract.

[0014] The preparation method of the magnolia officinalis extract comprises the following steps: mixing magnolia officinalis with water, heating and sterilizing, cooling, inoculating mold spores, fermenting, then adding a first solvent, extracting, filtering, to obtain a first extract liquid, and concentrating and drying the first extract liquid to obtain the magnolia officinalis extract.

[0015] The preparation method of the hogfujikawa extract includes the following steps: mixing hogfujikawa and water, sterilizing by heating, cooling, inoculating yeast strain, fermenting, then adding a second solvent, extracting, filtering, obtaining a second extract liquid, and drying the second extract liquid to obtain the hogfujikawa extract.

[0016] The second aspect of the present application provides a preparation method of an acne-removing composition, including the following steps:

[0017] (1) mixing magnolia bark and water, sterilizing by heating, cooling, inoculating mold strain, fermenting, then adding a first solvent, extracting, filtering, obtaining a first extract liquid, and drying the first extract liquid to obtain the magnolia bark extract;

[0018] (2) mixing hogfujikawa and water, sterilizing by heating, cooling, inoculating yeast strain, fermenting, then adding a second solvent, extracting, filtering, obtaining a second extract liquid, and drying the second extract liquid to obtain the hogfujikawa extract;

[0019] (3) mixing the magnolia bark extract obtained in step (1) and the hogfujikawa extract obtained in step (2) to obtain the acne-removing composition.

[0020] The third aspect of the present application provides the use of the acne-removing composition as described above in acne-removing cream, essence, and gel.

[0021] As described above, the acne-removing composition and the preparation method thereof have the following beneficial effects:

[0022] (1) The acne-removing composition of the present application can not only effectively inhibit the growth of key acne-causing bacteria such as propionibacterium acnes, but also regulate skin oil secretion, reduce the formation of acne, and have a whitening effect, reducing the pigmentation caused by acne and acne marks. The present application is particularly suitable for the skin characteristics of the youth group, aiming to solve the problem of acne from multiple angles, and at the same time, improve the overall health of the skin.

[0023] (2) The acne-removing composition of the present application has a comprehensive effect, not only can inhibit bacteria, but also can control oil and whiten, solve the problem of acne from multiple angles. At the same time, by using plant extracts, the irritation and side effects of chemical components on the skin are reduced, which is particularly suitable for sensitive skin of the youth group. Long-term use helps to improve the overall health of the skin and reduce the recurrence of acne. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 The figure shows the comparison chart of the user 1 of the present application before and after 2 days of trial.

[0025] Figure 2 The figure shows the comparison chart of the user 2 of the present application before and after 2 days of trial. DETAILED DESCRIPTION

[0026] In order to make the invention purposes, technical solutions and beneficial technical effects of the present application clearer, the present application will be further described in detail below in combination with embodiments, and other advantages and effects of the present application can be easily understood by those skilled in the art from the contents disclosed in the present specification.

[0027] The first aspect of the present application is to provide a composition for treating acne, which comprises an extract of giant knotweed and an extract of Magnolia officinalis;

[0028] The preparation method of the extract of Magnolia officinalis comprises the following steps: mixing Magnolia officinalis with water, sterilizing by heating, cooling, inoculating mold strains, fermenting, then adding a first solvent, extracting, filtering, to obtain a first extract solution, and drying the first extract solution to obtain the extract of Magnolia officinalis.

[0029] The preparation method of the extract of giant knotweed comprises the following steps: mixing giant knotweed with water, sterilizing by heating, cooling, inoculating yeast strains, fermenting, then adding a second solvent, extracting, filtering, to obtain a second extract solution, and drying the second extract solution to obtain the extract of giant knotweed.

[0030] In the present application, the plant is fermented by microorganisms. Under suitable fermentation conditions, the extracellular enzymes produced by the microorganisms can act on the plant to promote the release of active ingredients. Meanwhile, during the growth of the microorganisms, the active ingredients of traditional Chinese medicine are metabolized to produce new ingredients, which can further improve the efficacy of the plant.

[0031] In some embodiments of the present application, the mass ratio of the extract of giant knotweed to the extract of Magnolia officinalis is 3-9:2-6. Specifically, the mass ratio can be 3-6:2-5, 6-9:5-6; typically but not limitedly, for example, 3:2, 6:5, 1:2.

[0032] In some embodiments of the present application, the mass ratio of Magnolia officinalis to water is 1:3-8; specifically, the mass ratio can be 1:3-5, 1:5-8; typically but not limitedly, for example, 1:3, 1:5, 1:8.

[0033] In some embodiments of the present application, the inoculation temperature is room temperature.

[0034] In some embodiments of the present application, the mold strains are selected from one or more of Aspergillus oryzae or Monascus.

[0035] In some embodiments of the present application, the ratio of the amount of Magnolia officinalis added to the inoculation amount of the strain is 15-25g:0.5-1.5ml; specifically, the ratio of the amount of Magnolia officinalis added to the inoculation amount of the strain can be 15-20g:0.5-1ml, 20-25g:1-1.5ml; typically but not limitedly, for example, 15g:0.5ml, 20g:1ml, 25g:1.5ml.

[0036] In some embodiments of the application, the fermentation temperature is 35-40℃, and the fermentation time is 68-75h; specifically, the fermentation temperature can be 35-37℃, 37-40℃; typically but not limited to, for example, 35℃, 37℃, 40℃; and the fermentation time can be 68-72h, 72-75h; typically but not limited to, for example, 68h, 72h, 75h.

[0037] In some embodiments of the application, the first solvent is selected from one or more of ethanol or ethyl acetate.

[0038] In some embodiments of the application, the mass ratio of Magnolia officinalis to the first solvent is 1:15-30; specifically, the mass ratio can be 1:15-20, 1:20-30; typically but not limited to, for example, 1:15, 1:20, 1:30.

[0039] In some embodiments of the application, the extraction temperature is 60-80℃, and the extraction time is 1-3h. Specifically, the extraction temperature can be 60-70℃, 70-80℃; typically but not limited to, for example, 60℃, 70℃, 80℃.

[0040] In some embodiments of the application, the mass ratio of Polygonum cuspidatum to water is 1:8-15; specifically, the mass ratio can be 1:8-12, 1:12-15; typically but not limited to, for example, 1:8, 1:12, 1:15.

[0041] In some embodiments of the application, the inoculation temperature is room temperature.

[0042] In some embodiments of the application, the yeast strain is selected from one or more of Saccharomyces cerevisiae, Pichia pastoris or Candida.

[0043] In some embodiments of the application, the ratio of the amount of Polygonum cuspidatum added to the inoculation amount of the strain is 8-13g:0.5-1.5ml; specifically, the ratio of the amount of Polygonum cuspidatum added to the inoculation amount of the strain can be 8-10g:0.5-1ml, 10-13g:1-1.5ml; typically but not limited to, for example, 8g:0.5ml, 10g:1ml, 13g:1.5ml.

[0044] In some embodiments of the present application, the fermentation temperature is 25-35℃, and the fermentation time is 40-55h; specifically, the temperature can be 25-30℃, 30-35℃; typically but not limited to, for example, 25℃, 30℃, 35℃; the fermentation time can be 40-48h, 48-55h, typically but not limited to, for example, 40h, 48h, 55h.

[0045] In some embodiments of the present application, the second solvent is selected from one or more of ethanol or ethyl acetate.

[0046] In some embodiments of the present application, the mass ratio of the rhizoma magnoliae officinalis to the second solvent is 1:15-30; specifically, the mass ratio of the rhizoma magnoliae officinalis to the second solvent can be 1:15-23, 1:23-30; typically but not limited to, for example, 1:15, 1:23, 1:30.

[0047] In some embodiments of the present application, the extraction temperature is 50-70℃, and the extraction time is 1-3h.

[0048] The second aspect of the present application provides a preparation method of the acne-removing composition, comprising the following steps:

[0049] (1) mixing the rhizoma magnoliae officinalis with water, heating and sterilizing, cooling, inoculating mold strains, fermenting, then adding the first solvent, extracting, filtering, to obtain a first extract, concentrating and drying the first extract to obtain a rhizoma magnoliae officinalis extract;

[0050] (2) mixing the rhizoma magnoliae officinalis with water, heating and sterilizing, cooling, inoculating mold strains, fermenting, then adding the first solvent, extracting, filtering, to obtain a first extract, concentrating and drying the first extract to obtain a rhizoma magnoliae officinalis extract;

[0051] (3) mixing the rhizoma magnoliae officinalis extract obtained in step (1) and the rhizoma magnoliae officinalis extract obtained in step (2) to obtain the acne-removing composition.

[0052] The preparation method of the present application involves the same conditions as described above, which is not repeated here.

[0053] The third aspect of the present application provides the use of the acne-removing composition as described above in acne-removing cream, essence, and gel.

[0054] The present application will be further described below by way of examples, but the scope of the present application is not limited thereto.

[0055] When the embodiments give a numerical range, it should be understood that, unless otherwise specified by the present application, both ends of each numerical range and any one numerical between the two ends can be selected. Unless otherwise defined, all technical and scientific terms used in the present application have the same meaning as commonly understood by those skilled in the art. Unless otherwise noted in the embodiments, the conventional conditions or the conditions recommended by the manufacturer are used. All reagents or instruments, unless the manufacturer is noted, are conventional products that can be obtained by commercial purchase. In addition to the specific methods, devices, materials used in the embodiments, any method, device and material of the prior art similar or equivalent to the methods, devices and materials described in the embodiments of the present application can also be used to implement the present application according to the mastery of the prior art by those skilled in the art and the description of the present application.

[0056] The magnolia officinalis and the polygonum cuspidatum root of the present application meet the pharmacopoeia standard.

[0057] The spore suspension quantity of the mold species of the present application is 1x10 7 cfu / mL.

[0058] The viable bacteria quantity of the yeast species of the present application is 1x10 7 cfu / mL.

[0059] The strains used in the present application are all commercially available strains. The aspergillus oryzae strain is numbered as BNCC338380; the monascus purpureus strain is numbered as BNCC190637; the saccharomyces cerevisiae strain is numbered as BNCC336048; the pichia pastoris strain is numbered as BNCC336048; the candida tropicalis strain is numbered as BNCC376420; the escherichia coli strain of the present application is numbered as BNCC363342; the bacillus subtilis strain of the present application is numbered as BNCC190068, and the above-mentioned strains are manufactured by Shangcheng Beinaeliang Biotechnology Co., Ltd.

[0060] Preparation example of magnolia officinalis extract

[0061] Preparation example A1

[0062] The present preparation example provides a preparation method of magnolia officinalis extract, which comprises the following steps: after the magnolia officinalis is crushed, it is passed through a 40-mesh sieve, 20g is weighed in a triangular flask, 5 times of water is added, sterilization is carried out in a high-pressure sterilization pot at 121℃ for 20min, after the room temperature is recovered, 1ml of aspergillus oryzae strain is inoculated, and fermentation is carried out in a constant temperature incubator at 37℃ for 72h, after the fermentation is completed, the medicinal material is added with 20 times of 50% ethanol, and extraction is carried out at 70℃ for 2h, filtration is carried out, and the extraction liquid is obtained, the extraction liquid is concentrated and dried, and the magnolia officinalis extract is obtained.

[0063] Preparation example A2

[0064] The preparation example provides a preparation method of Magnolia officinalis extract, and the difference from the preparation example A1 is that Escherichia coli fermentation is adopted.

[0065] Preparation example A3

[0066] The preparation example provides a preparation method of Magnolia officinalis extract, and the difference from the preparation example A1 is that conventional extraction process is adopted. Specifically, after Magnolia officinalis is crushed and passed through a 40-mesh sieve, 20 g is weighed in a beaker, 20 times 50% ethanol is added, and extraction is carried out at 70 DEG C for 2 h; filtration is carried out to obtain an extract, and the extract is concentrated and dried to obtain Magnolia officinalis extract.

[0067] Preparation example A4

[0068] The commercially available Magnolia officinalis extract is from Bozhou Xier Chinese Herbal Medicine Co., Ltd.

[0069] Preparation example A5

[0070] The preparation example provides a preparation method of Magnolia officinalis extract, and the difference from the preparation example A1 is that conventional extraction process is adopted. Specifically, after Magnolia officinalis is crushed and passed through a 40-mesh sieve, 20 g is weighed in a beaker, 20 times 50% ethanol is added, and extraction is carried out at 70 DEG C for 2 h; filtration is carried out to obtain an extract, and the extract is concentrated and dried to obtain Magnolia officinalis extract.

[0071] Preparation example of Polygonum cuspidatum extract

[0072] Preparation example B1

[0073] The preparation example provides a preparation method of Polygonum cuspidatum extract, and the difference from the preparation example A1 is that conventional extraction process is adopted. Specifically, after Magnolia officinalis is crushed and passed through a 40-mesh sieve, 20 g is weighed in a beaker, 20 times 50% ethanol is added, and extraction is carried out at 70 DEG C for 2 h; filtration is carried out to obtain an extract, and the extract is concentrated and dried to obtain Magnolia officinalis extract.

[0074] Preparation example B2

[0075] The preparation example provides a preparation method of Polygonum cuspidatum extract, and the difference from the preparation example A1 is that conventional extraction process is adopted. Specifically, after Magnolia officinalis is crushed and passed through a 40-mesh sieve, 20 g is weighed in a beaker, 20 times 50% ethanol is added, and extraction is carried out at 70 DEG C for 2 h; filtration is carried out to obtain an extract, and the extract is concentrated and dried to obtain Magnolia officinalis extract.

[0076] Preparation example B3

[0077] The preparation example provides a preparation method of a rhizoma polygoni multifidi extract, which is different from the preparation example A1 in that a conventional extraction process is used. Specifically, 10 g of the rhizoma polygoni multifidi powder is weighed in a beaker, 20 times of 80% ethanol is added, and extraction is performed at 60°C for 2h. Filtration is performed to obtain an extract, and the extract is concentrated and dried to obtain the rhizoma polygoni multifidi extract.

[0078] Preparation example B4

[0079] The rhizoma polygoni multifidi extract is commercially available, and the manufacturer is Bozhou Xier Chinese Herbal Medicine Co., Ltd.

[0080] Preparation example B5

[0081] The preparation example provides a preparation method of a rhizoma polygoni multifidi extract, which includes the following steps: the rhizoma polygoni multifidi root is crushed and passed through a 40-mesh sieve, 10 g of the rhizoma polygoni multifidi powder is weighed in a triangular flask, 15 times of water is added, sterilization is performed in a high-pressure sterilization pot at 121°C for 20 min, after the temperature is restored to room temperature, 1 ml of a pichia yeast seed is inoculated, and fermentation is performed in a shaking bed at 30°C for 55 h at a speed of 180 r / min. After the fermentation is completed, the medicinal material is added to 30 times of 80% ethanol, extraction is performed at 60°C for 2h, filtration is performed to obtain an extract, and the extract is concentrated and dried to obtain the rhizoma polygoni multifidi extract.

[0082] Embodiment

[0083] Embodiment 1

[0084] The embodiment provides an anti-acne composition, which is composed of magnolia officinalis extract and rhizoma polygoni multifidi extract at a mass ratio of 3:2. The magnolia officinalis extract is prepared by the preparation example A1, and the rhizoma polygoni multifidi extract is prepared by the preparation example B1.

[0085] Embodiment 2

[0086] The embodiment provides an anti-acne composition, which is different from the embodiment 1 in that the magnolia officinalis extract is prepared by the preparation example A2, and the rhizoma polygoni multifidi extract is prepared by the preparation example B2.

[0087] Embodiment 3

[0088] The embodiment provides an anti-acne composition, which is different from the embodiment 1 in that the magnolia officinalis extract is prepared by the preparation example A3, and the rhizoma polygoni multifidi extract is prepared by the preparation example B3.

[0089] Embodiment 4

[0090] The embodiment provides an anti-acne composition, which is different from the embodiment 1 in that the magnolia officinalis extract is prepared by the preparation example A4, and the rhizoma polygoni multifidi extract is prepared by the preparation example B4.

[0091] Embodiment 5

[0092] The embodiment provides a composition for removing acne, which is different from the composition for removing acne in the embodiment 1 in that the composition for removing acne is composed of Magnolia officinalis extract and Polygonum cuspidatum extract in a mass ratio of 1:3.

[0093] Embodiment 6

[0094] The embodiment provides a composition for removing acne, which is different from the composition for removing acne in the embodiment 1 in that the composition for removing acne is Magnolia officinalis extract.

[0095] Embodiment 7

[0096] The embodiment provides a composition for removing acne, which is different from the composition for removing acne in the embodiment 1 in that the composition for removing acne is Polygonum cuspidatum extract.

[0097] Embodiment 8

[0098] The embodiment provides a composition for removing acne, which is different from the composition for removing acne in the embodiment 1 in that the composition for removing acne is composed of Magnolia officinalis extract, Polygonum cuspidatum extract and Phellodendri chinensis bark extract in a mass ratio of 3:2:2. The Phellodendri chinensis bark extract is commercially available.

[0099] Embodiment 9

[0100] The embodiment provides a composition for removing acne, which is different from the composition for removing acne in the embodiment 1 in that the Magnolia officinalis extract is prepared in the preparation example A5, and the Polygonum cuspidatum extract is prepared in the preparation example B5.

[0101] Performance test

[0102] 1. Bacteriostatic test

[0103] The bacteriostatic effect of the compositions for removing acne prepared in the embodiments 1-9 on Staphylococcus aureus and Propionibacterium acnes is tested by using the Oxford cup bacteriostatic ring method, the concentration of the tested composition is 1% composition aqueous solution, the testing method is the bacteriostatic ring method in WS / T 650, and the results are shown in Table 1.

[0104] Table 1: The antibacterial performance of the compositions for removing acne prepared in the embodiments 1-9

[0105] No. Propionibacterium acnes BNCC330605 (mm) Staphylococcus aureus ATCC6538 (mm) Example 1 14.6 12.5 Example 2 8.9 10.3 Example 3 9 9.2 Example 4 9.4 8.5 Example 5 13.5 12.1 Example 6 12.1 10.7 Example 7 11.5 11.9 Example 8 13.9 11.5 Example 9 13.7 13.0

[0106] The composition for removing acne prepared in the embodiment has better inhibition performance on Propionibacterium acnes and better antibacterial performance, and the acne-removing effect can be further improved.

[0107] 2. Oil control performance test

[0108] The inhibition rate of the sample on 5a-reductase is detected by using an enzyme-linked immunosorbent assay (ELISA) to evaluate the oil control activity, and the sample is tested at a concentration of 10 mg / mL. The enzyme-linked immunosorbent assay (ELISA) is a routine knowledge in the field, and the testing is performed by using a routine method in the field, and the specific process is as follows.

[0109] 1) The sample was diluted with 0.1M tris-HCl buffer to a sample concentration of 10mg / ml, as a sample for the next step test;

[0110] 2) Set up blank, positive control hole, sample hole, negative control hole; except that the blank group does not add enzyme, each hole of other groups adds 50uL of 5a-reductase, incubates in a 37℃ constant temperature box for 10min; the sample hole adds the sample, and the positive control hole adds the positive control; the micro-hole plate after adding the sample is incubated for 10min, and the plate is washed 5 times (the washing solution is provided by the kit, and the original washing solution is diluted 20 times for use); the positive control is 1uM of finasteride; and the kit is a DHT detection kit;

[0111] 3) 100uL of horseradish peroxidase (HRP) labeled detection antibody is added to each hole of the negative control group, the positive control group and the sample hole, the reaction hole is sealed with a sealing film, and is incubated in a 37℃ water bath or a constant temperature box for 60min. Discard the liquid and pat dry on a water paper, and wash the plate 3 times with a plate washing machine. 50uL of substrate A and B are added to each hole, and incubated at 37℃ in the dark for 15min; the substrate A is 0.2mM of testosterone; and the substrate B is 1mM of NADPH;

[0112] 4) 50uL of a termination liquid is added to each hole, and the OD value of each hole is determined at 450nm wavelength within 15min, and the percentage of the sample inhibition rate is calculated according to the following formula: inhibition rate (%) = 100% x (OD 空白 + OD 对照组 - OD 样品 ) / (OD 空白 + OD 对照组 ). The percentage of the positive control inhibition rate is calculated according to the following formula: inhibition rate (%) = 100% x (OD 空白 + OD 对照组 - OD 阳性对照 ) / (OD 空白 + OD 对照组 ). The termination liquid is a kit termination liquid with a concentration of 0.1M.

[0113] Table 2 5a-reductase inhibition rate test results of the acne-removing compositions of examples 1-9

[0114] No. 5a-reductase inhibition rate % Negative control 3.56 Positive control 82.47 Example 1 73.93 Example 2 55.21 Example 3 49.58 Example 4 50.25 Example 5 70.41 Example 6 62.65 Example 7 64.29 Example 8 68.31 Example 9 71.45

[0115] The acne-removing composition prepared in the application has a higher inhibition rate on 5a-reductase and a better oil control effect.

[0116] 3, whitening performance test

[0117] Reference to the group standard T / SHRH 015-2018 "Cosmetics - Experimental method for inhibiting tyrosinase activity", test the inhibitory activity of the acne-removing compositions of Examples 1-9 on tyrosinase at a concentration of 10 mg / mL. The test results are shown in Table 3.

[0118] Table 3 Test results of tyrosinase inhibition of acne-removing compositions of Examples 1-9

[0119]

[0120]

[0121] The acne-removing compositions prepared by the present application have a high inhibition rate on tyrosinase and have a whitening effect.

[0122] 4. Acne-removing effect experiment

[0123] Table 4 Serum formulation

[0124] Ingredient Content / % Xanthan gum 0.1 Anti-acne composition (Example 1) 0.1 Phenoxyethanol 0.45 Ethylhexylglycerin 0.05 Propylene glycol 3 Water Balance

[0125] The above Example 1 is directly prepared into a serum formulation, and the formulation is shown in Table 4. As a serum, it is directly applied to the affected area when used. It can also be used on the whole face.

[0126] According to the "Clinical Evaluation Standard for Acne-removing Efficacy Skin Care Products" in the "China Non-public Medical Institutions Association Group Standard" (T / CNMIA0012-2020), 20 volunteers with facial acne, aged 18-35, were recruited. The participants used the acne-removing serum prepared by Example 1 every day for 7 consecutive days.

[0127] I. Symptom improvement evaluation:

[0128] No other skin care products of the same type were used during the experiment, and all the subjects completed the verification process. The experimental results are shown in Table 5, in which:

[0129] Cure: lesion regression rate ≥ 90%;

[0130] Marked effect: lesion regression rate 60-89%;

[0131] Improvement: lesion regression rate 20-59%;

[0132] No effect: lesion regression rate ≤ 20%.

[0133] Table 5 Acne-removing experiment effect of acne-removing composition of Example 1

[0134]

[0135] The effect comparison chart of some volunteers before and after use is as follows Figure 1 andFigure 2 The results are shown in Table 1.

[0136] From the above results, it can be seen that the acne-removing composition of the present application has good bacteriostatic and acne-removing effects.

[0137] The above examples are only illustrative of the principles and effects of the present application, and are not intended to limit the present application. Any person skilled in the art can modify or change the above examples without departing from the spirit and scope of the present application. Therefore, all equivalent modifications or changes made by those skilled in the art without departing from the spirit and technical ideas disclosed by the present application should be covered by the claims of the present application.

Claims

1. An acne treatment composition characterized in that, The composition comprises a hogfujikawa extract and a magnolia officinalis extract; The preparation method of the magnolia officinalis extract comprises the following steps: mixing the magnolia officinalis with water, sterilizing by heating, cooling, inoculating mold strains, fermenting, then adding a first solvent, extracting, filtering, obtaining a first extract, concentrating and drying the first extract, and obtaining the magnolia officinalis extract. The preparation method of the hogfujikawa extract comprises the following steps: mixing the hogfujikawa with water, sterilizing by heating, cooling, inoculating yeast strains, fermenting, then adding a second solvent, extracting, filtering, obtaining a second extract, concentrating and drying the second extract, and obtaining the hogfujikawa extract.

2. The anti-acne composition according to claim 1, characterized in that: The mass ratio of the hogfujikawa extract and the magnolia officinalis extract is 3-9:2-6.

3. The acne treatment composition of claim 1, wherein: The preparation method of the magnolia officinalis extract further comprises one or more of the following features: 11) the mass ratio of the magnolia officinalis to water is 1:3-8; 12) the sterilizing temperature is 115-121 DEG C, and the sterilizing time is 15-30 min; 13) the inoculating temperature is room temperature; 14) the mold strains are selected from one or more of aspergillus oryzae and monascus; 15) the ratio of the added amount of the magnolia officinalis to the inoculating amount of the strains is 15-25 g:0.5-1.5 ml; 16) the fermenting temperature is 35-40 DEG C, and the fermenting time is 68-75 h; 17) the first solvent is selected from one or more of ethanol or ethyl acetate; 18) the mass ratio of the magnolia officinalis to the first solvent is 1:15-30; 19) the extracting temperature is 60-80 DEG C, and the extracting time is 1-3 h.

4. The acne treatment composition of claim 1, wherein: The preparation method of the hogfujikawa extract further comprises one or more of the following features: 21) the mass ratio of the hogfujikawa to water is 1:8-15; 22) the sterilizing temperature is 115-121 DEG C, and the sterilizing time is 15-30 min; 23) the inoculating temperature is room temperature; 24) the yeast strains are selected from one or more of saccharomyces cerevisiae, pichia pastoris or candida; 25) the ratio of the added amount of the hogfujikawa to the inoculating amount of the strains is 8-13 g:0.5-1.5 ml; 26) the fermenting temperature is 25-35 DEG C, and the fermenting time is 40-55 h; 27) the second solvent is selected from one or more of ethanol or ethyl acetate; 28) the mass ratio of the hogfujikawa to the second solvent is 1:15-30.

5. The acne treatment composition of claim 1, wherein: The extracting temperature in the preparation method of the hogfujikawa extract is 50-70 DEG C, and the extracting time is 1-3 h.

6. A method of preparing the anti-acne composition according to any one of claims 1 to 5, characterized in that, The preparation method comprises the following steps: (1) mixing the magnolia officinalis with water, sterilizing by heating, cooling, inoculating mold strains, fermenting, then adding a first solvent, extracting, filtering, obtaining a first extract, concentrating and drying the first extract, and obtaining the magnolia officinalis extract; (2) mixing the hogfujikawa with water, sterilizing by heating, cooling, inoculating yeast strains, fermenting, then adding a second solvent, extracting, filtering, obtaining a second extract, concentrating and drying the second extract, and obtaining the hogfujikawa extract; (3) mixing the magnolia officinalis extract obtained in step (1) and the hogfujikawa extract obtained in step (2), and obtaining the acne-removing composition.

7. The production method according to claim 6, wherein The step (1) further comprises one or more of the following features: 11) the mass ratio of Magnolia officinalis to water is 1:3-8; 12) the sterilization temperature is 115-121℃, and the sterilization time is 15-30min; 13) the inoculation temperature is room temperature; 14) the mold strain is selected from one or more of Aspergillus oryzae and Monascus; 15) the ratio of the amount of Magnolia officinalis to the inoculation amount of the strain is 15-25g:0.5-1.5ml; 16) the fermentation temperature is 35-40℃, and the fermentation time is 68-75h; 17) the first solvent is selected from one or more of ethanol or ethyl acetate; 18) the mass ratio of Magnolia officinalis to the first solvent is 1:15-30; 19) the extraction temperature is 60-80℃, and the extraction time is 1-3h.

8. The preparation method according to claim 6, characterized in that, In the step (2), one or more of the following features is further included: 21) the mass ratio of Polygonum cuspidatum to water is 1:8-15; 22) the sterilization temperature is 115-121℃, and the sterilization time is 15-30min; 23) the inoculation temperature is room temperature; 24) the yeast strain is selected from one or more of Saccharomyces cerevisiae, Pichia pastoris or Candida; 25) the ratio of the amount of Polygonum cuspidatum to the inoculation amount of the strain is 8-13g:0.5-1.5ml; 26) the fermentation temperature is 25-35℃, and the fermentation time is 40-55h; 27) the second solvent is selected from one or more of ethanol or ethyl acetate; 28) the mass ratio of Polygonum cuspidatum to the second solvent is 1:15-30; 29) the extraction temperature is 50-70℃, and the extraction time is 1-3h.

9. The preparation method according to claim 6, characterized in that, In the step (3), the mass ratio of Polygonum cuspidatum extract to Magnolia officinalis extract is 3-9:2-6.

10. The use of the acne-removing composition according to any one of claims 1-5 in acne-removing cream, essence, gel.

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