A composition with soothing and antipruritic efficacy, and a preparation method and application thereof

Abstract

The application discloses a composition with soothing and itching-relieving effects and a preparation method and application thereof, and belongs to the cosmetic field; the composition comprises the following components: a composite plant extract, a polyhydric alcohol and water; the composite plant extract is compounded by a polygonatum sibiricum redoute fermentation liquor, a paeonia extract and a long-stemmed grape caulerpa lentillifera extract; and the polygonatum sibiricum redoute fermentation liquor is obtained by composite fermentation of lactobacillus pentosus and bacillus subtilis. The soothing and itching-relieving composition provided by the application has excellent soothing effects, and the soothing and itching-relieving composition provided by the application has excellent in-vitro hyaluronidase inhibition effects, and the in-vitro hyaluronidase inhibition rate reaches more than 60%.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of cosmetics, and particularly relates to a composition with soothing and antipruritic effects and a preparation method and application thereof. BACKGROUND

[0002] Skin itching is a common symptom of various skin diseases (such as eczema, dermatitis, urticaria, etc.) and allergic reactions, which seriously affects the quality of life of patients. At present, chemical synthetic ingredients such as glucocorticoids and antihistamines are commonly used in clinical treatment, which can quickly relieve symptoms, but long-term use can easily cause side effects such as skin atrophy, capillary dilation, drug resistance, and cannot solve the problem of deep inflammation and skin barrier repair. In recent years, natural plant extracts have gradually attracted attention due to their high safety and small side effects, such as glycyrrhizic acid, aloe polysaccharide, and purslane extract, which have been applied in soothing products. However, the antipruritic effect of single active ingredient in the prior art is limited, and when multiple ingredients are compounded, there are often problems such as insufficient synergistic effect, poor stability, and low transdermal absorption efficiency. In addition, traditional preparation processes often use high temperature or organic solvent extraction, which can easily cause inactivation of heat-sensitive active ingredients and reduce product efficacy; the dosage form design is also relatively single, and it is difficult to meet the dual needs of rapid antipruritic and long-term repair.

[0003] Therefore, developing a composition with multiple active ingredients synergistically, mild and efficient preparation process, and both instant soothing and long-term repair functions has become an important direction to solve the defects of the prior art. SUMMARY

[0004] In view of the deficiencies of the prior art, the purpose of the present application is to provide a composition with synergistic effect of multiple active ingredients, which is prepared by fermentation process combined with traditional solvent extraction process, and has the effects of soothing and repairing, anti-allergic and antipruritic.

[0005] In order to achieve the above-mentioned purpose, the following technical solutions are disclosed:

[0006] In a first aspect, the present application provides a composition with soothing and antipruritic effects, comprising the following components by mass percentage:

[0007] Compound plant extract 13-15%;

[0008] Polyhydric alcohol 3-8%;

[0009] Water, supplemented to 100%;

[0010] The compound plant extract is compounded by the fermentation liquor of polygonatum sibiricum, the extract of paeonia lactiflora and the extract of long-stem grape caulerpa;

[0011] The preparation method of the fermentation liquor of polygonatum sibiricum includes the following steps:

[0012] Step 1-1. Take Polygonatum rhizome, crush it, and sieve it to obtain Polygonatum rhizome powder;

[0013] Step 1-2. Mix Polygonatum powder and water at a material-to-liquid ratio of 1:(10-15) g / mL to obtain the fermentation substrate;

[0014] Steps 1-3. Take the compound bacterial solution of Lactobacillus pentosus and Bacillus subtilis and add it to the fermentation substrate at an inoculation rate of 8-10 v / v%. Fermentation temperature is 30-37℃, stirring speed is 100-150 r / min, aeration rate is 0.5-1 vvm, fermentation culture is carried out for 2-3 days. After fermentation is completed, sterilize at 121℃ for 20 min, cool and centrifuge to obtain Polygonatum sibiricum fermentation broth.

[0015] Preferably, the viable count of *Lactobacillus pentosus* in the compound bacterial solution is (1-2) × 10⁻⁶. 8 CFU / mL, Bacillus subtilis viable count was (5-6) × 10⁻⁶ 8 CFU / mL.

[0016] More preferably, the preparation method of the peony extract includes the following steps:

[0017] Step 2-1. Take a certain amount of peony powder, add water and soak for 12-16 hours, soaking temperature 25-35℃, material-to-water ratio 1:(10-15)g / mL. After soaking, centrifuge to separate filtrate 1 and filter residue.

[0018] Step 2-2. Add 70 v / v% ethanol aqueous solution to the filter residue, with a material-to-liquid ratio of 1: (20-25) g / mL. Extract in a water bath at 120-140℃ for 120-140 min. After extraction, centrifuge to obtain filtrate 2. Combine filtrate 2 with filtrate 1, concentrate and dry under reduced pressure to obtain peony extract.

[0019] More preferably, the preparation method of the *Botrytis cinerea* extract includes the following steps:

[0020] Step 3-1. Crush and sieve the dried long-stemmed grape aquatic plants to obtain long-stemmed grape aquatic plant powder for later use;

[0021] Step 3-2. Mix the powder of *Gnaphalium longifolium* with a 75% v / v% ethanol aqueous solution at a material-to-liquid ratio of 1:(30-45) g / mL, and extract in a water bath at 60-70℃ for 100-120 min. After extraction, centrifuge to separate the residue and retain the liquid. Concentrate and dry under reduced pressure to obtain *Gnaphalium longifolium* extract.

[0022] More preferably, the compound plant extract is composed of Polygonatum sibiricum fermentation liquid, Paeonia lactiflora extract and Gracilaria longiflora extract in a mass ratio of 10:(1-4):1.

[0023] Preferably, the polyol is at least one of glycerol, 1,3-propanediol, 1,2-hexanediol.

[0024] In a second aspect, the present application provides use of the composition having soothing and anti-itching effects according to the first aspect in the preparation of a skin care product having soothing, anti-irritation and / or repairing effects.

[0025] In a third aspect, the present application provides a skin resident agent having soothing and anti-itching effects, comprising the composition having soothing and anti-itching effects according to the first aspect.

[0026] The composition having soothing and anti-itching effects is added to the skin resident agent in an amount of 5-10 wt%.

[0027] Preferably, the composition further comprises a humectant, an emollient, a preservative, a pH adjuster, an emulsifier, a solvent.

[0028] In the present application:

[0029] After the Huangjing is fermented by the complex fermentation of lactobacillus pentosus and bacillus subtilis, active ingredients (such as polysaccharides, saponins, flavonoids) are degraded into small molecule oligosaccharides, short chain fatty acids and isoflavone aglycone, etc., which significantly improve the transdermal absorption efficiency, inhibit the release of inflammatory factors (such as TNF-α), regulate Th1 / Th2 immune balance and block the transmission of histamine, and synergistically play the roles of anti-inflammatory, anti-itching and anti-allergic; at the same time, a small amount of organic acid (lactic acid, acetic acid) in the fermentation product can repair the skin barrier and enhance the moisturizing property. The Huangjing fermentation broth prepared by the fermentation process avoids the destruction of part of the active ingredients by high temperature or organic solvents, so that the product has both mildness and high efficiency.

[0030] The Paeonia extract contains rich paeoniflorin, flavonoids and polysaccharides, etc., and plays the roles of soothing, anti-itching and anti-allergic: paeoniflorin can inhibit the release of inflammatory mediators (such as TNF-α), reduce histamine sensitivity and relieve redness, itching and swelling; flavonoid components can scavenge free radicals, reduce oxidative stress damage, and tannins can soothe and calm red skin; at the same time, polysaccharide substances can strengthen the skin barrier function and reduce the penetration of external stimuli.

[0031] The Caudium japonicum extract contains rich polyphenol compounds, which have the effects of soothing, anti-itching and anti-allergic, and the polyphenol compounds can scavenge free radicals and inhibit the release of histamine, thereby relieving itching and sensitive reactions caused by external stimuli (such as ultraviolet rays and pollutants).

[0032] The beneficial effects of the present application are:

[0033] 1. The soothing and itching-relieving composition provided by the present application has excellent soothing effect, and the content of TNF-alpha inflammatory factor in macrophages treated by the soothing and itching-relieving composition containing the polygonatum sibiricum ferment, the peony extract and the caulerpa lentillifera extract is significantly reduced compared with the negative control group, and the TNF-alpha inhibition rate reaches 38.26%, 41.46% and 40.29% respectively;

[0034] 2. The soothing and itching-relieving composition provided by the present application has significantly improved hyaluronidase inhibition rate compared with the composition lacking any one of the components, and the in-vitro hyaluronidase inhibition rate reaches more than 60%, indicating that the polygonatum sibiricum ferment, the peony extract and the caulerpa lentillifera extract can promote each other when used in combination, so that the composition has significant soothing, anti-allergic and itching-relieving effect.

[0035] 3. In the present application, the polygonatum sibiricum ferment is prepared by fermentation of the complex bacteria composed of lactobacillus pentosus and bacillus subtilis, and the soothing and itching-relieving composition prepared using the ferment has significantly improved soothing and anti-allergic effect compared with the composition prepared using the polygonatum sibiricum water extract or the polygonatum sibiricum ferment produced by fermentation of a single strain, indicating that the two functional bacteria in the complex bacteria used in the present application can influence and promote each other when mixed fermentation, so that the product has both mildness and high efficiency. DETAILED DESCRIPTION

[0036] To better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples. Those skilled in the art should understand that the specific examples described herein are only used to explain the present application, and are not used to limit the present application.

[0037] The test methods used in the examples and comparative examples are conventional methods unless otherwise specified; the materials, reagents, etc. used are commercially available unless otherwise specified; the percentages mentioned in the examples and comparative examples are mass percentages unless otherwise specified.

[0038] In the present application:

[0039] Polygonatum sibiricum: obtained from the self-owned planting base of Shanghai Jingxin Biotechnology Co., Ltd.;

[0040] Peony: obtained from the self-owned planting base of Shanghai Jingxin Biotechnology Co., Ltd.;

[0041] Caulerpa lentillifera: purchased from Shenzhen Lanting Dingzhi Biological Technology Co., Ltd.;

[0042] Lactobacillus pentosus: purchased from China General Microbiological Culture Collection Center, with the preservation number of CGMCC 1.2707;

[0043] Bacillus subtilis: purchased from China General Microbiological Culture Collection Center, with the preservation number of CGMCC NO. 1.821.

[0044] Preparation of the fermented liquid of Rhizoma Polygonati

[0045] The preparation method of the fermented liquid of Rhizoma Polygonati 1 comprises the following steps:

[0046] Step 1-1. The Rhizoma Polygonati root slices are dried to constant weight, crushed in a crusher, and sieved through a 30-mesh sieve to obtain Rhizoma Polygonati powder;

[0047] Step 1-2. The Rhizoma Polygonati powder obtained in step 1-1 is mixed with water at a solid-liquid ratio of 1:13 g / mL to obtain a fermentation substrate, which is autoclaved at 121°C for 20 min for standby;

[0048] Step 1-3. The complex bacterial liquid of Lactobacillus pentosus and Bacillus subtilis is inoculated into the fermentation substrate at an inoculation amount of 10 v / v%, the fermentation temperature is 37°C, the stirring speed is 150 r / min, the aeration amount is 0.8 vvm, and the fermentation culture is carried out for 3 days. After the fermentation is completed, sterilization is carried out at 121°C for 20 min, and centrifugation is carried out after cooling to obtain the fermented liquid of Rhizoma Polygonati 1, wherein the viable count of Lactobacillus pentosus in the complex bacterial liquid is 1×10 8 CFU / mL, and the viable count of Bacillus subtilis is 5×10 8 CFU / mL.

[0049] The fermented liquid of Rhizoma Polygonati 2: Compared with the preparation method of the fermented liquid of Rhizoma Polygonati 1, the only difference is that the complex bacterial liquid inoculated in step 1-3 is replaced by Lactobacillus pentosus liquid inoculated alone, and the viable count of the inoculum and the remaining conditions and parameters are consistent with those of the preparation method of the fermented liquid of Rhizoma Polygonati 1.

[0050] The fermented liquid of Rhizoma Polygonati 3: Compared with the preparation method of the fermented liquid of Rhizoma Polygonati 1, the only difference is that the complex bacterial liquid inoculated in step 1-3 is replaced by Bacillus subtilis liquid inoculated alone, and the viable count of the inoculum and the remaining conditions and parameters are consistent with those of the preparation method of the fermented liquid of Rhizoma Polygonati 1.

[0051] The water extract of Rhizoma Polygonati: The Rhizoma Polygonati root slices are dried to constant weight, crushed in a crusher, and sieved through a 30-mesh sieve to obtain Rhizoma Polygonati powder. The Rhizoma Polygonati powder is mixed with deionized water at a solid-liquid ratio of 1:20 g / mL, soaked at 35°C for 12 h, and then centrifuged to separate the residue and obtain the water extract of Rhizoma Polygonati.

[0052] Preparation of the extract of Paeonia lactiflora

[0053] Step 2-1. The Paeonia lactiflora is dried to constant weight, crushed in a crusher, and sieved through a 30-mesh sieve to obtain Paeonia lactiflora powder. The Paeonia lactiflora powder is soaked with water, the soaking time is 14 h, the soaking temperature is 30°C, the solid-liquid ratio is 1:15 g / mL, and after the soaking is completed, centrifugal separation is performed to obtain filtrate 1 and filter residue;

[0054] Step 2-2. The residue was added with 70v / v% ethanol aqueous solution, the solid-liquid ratio was 1:25 g / mL, and extraction was performed in a 65°C water bath for 140 min. After extraction, the filtrate 2 was obtained by centrifugal separation. The filtrate 2 was combined with the filtrate 1, and concentrated under reduced pressure to dryness to obtain the peony extract with a water content of 23-25%.

[0055] Preparation of long-stalk grape caulerpa extract

[0056] Step 3-1. The dried long-stalk grape caulerpa was crushed through a 60-mesh sieve to obtain long-stalk grape caulerpa powder for use.

[0057] Step 3-2. The long-stalk grape caulerpa powder was mixed with 75v / v% ethanol aqueous solution at a solid-liquid ratio of 1:40 g / mL. After extraction in a 70°C water bath for 100 min, the residue was removed by centrifugal separation, and the filtrate was concentrated under reduced pressure to dryness to obtain the long-stalk grape caulerpa extract with a water content of 8-10%.

[0058] Preparation of composite plant extract

[0059] The composite plant extract was prepared by compounding the fermented liquid of rhizoma polygonati, the peony extract, and the long-stalk grape caulerpa extract at a mass ratio of 10:(1-4):1. See Table 1 for details.

[0060] Table 1 Raw material ratio of composite plant extract

[0061] Raw materials Composite plant extract 1 Composite plant extract 2 Composite plant extract 3 Composite plant extract 4 Composite plant extract 5 Composite plant extract 6 Composite plant extract 7 Composite plant extract 8 Rhizoma polygonati fermentation broth 1 10 10 10 10 10 - Paeonia extract 1 3 4 - 3 3 3 3 Caulis adianti extract 1 1 1 1 - 1 1 1 Rhizoma polygonati water extract - - - - - 10 - - Rhizoma polygonati fermentation broth 2 - - - - - - 10 - Rhizoma polygonati fermentation broth 3 - - - - - - - 10

[0062] Note: “-” in the table indicates no addition of the corresponding component, and the amount of the missing component is proportionally distributed to the remaining components; and the total amount of the composite plant extract is the same.

[0063] Preparation of soothing and antipruritic composition

[0064] The raw materials were accurately weighed according to the mass percentage in Table 2, and placed in a high-speed homogenizer for homogenization and dispersion for 15 min to obtain the soothing and antipruritic composition.

[0065] Table 2 Mass percentage of raw materials of soothing and antipruritic composition

[0066] Raw materials Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 Composition 7 Composition 8 Composite plant extract / % 13 14 15 14 14 14 14 14 Composite plant extract serial number Extract 1 Extract 2 Extract 3 Extract 4 Extract 5 Extract 6 Extract 7 Extract 8 Glycerin / % 3 5 5 5 5 5 5 5 1,3-propanediol / % - - 1 - - - - - 1,2-hexanediol / % - - 2 - - - - - Deionized water / % Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100 Supplemented to 100

[0067] Note: “-” in the table indicates no addition.

[0068] Performance test:

[0069] To verify the soothing and antipruritic effect of the composite plant extract and the relationship between the components in the composite plant extract, the composite plant extract 2 formula was modified or replaced to obtain the composite plant extracts 4-8. See Table 1 for details. The composite plant extracts 1-8 were prepared into soothing and antipruritic compositions 1-8, which were then subjected to the following performance tests.

[0070] 1 Soothing efficacy test

[0071] (1) Test basis: This experiment is designed and tested according to the standard of “T / SHRH034-2021 Cosmetics Soothing Efficacy Test - In Vitro TNF-α Inflammatory Factor Content Determination Lipopolysaccharide Induced Macrophage Cell Line Inflammatory Cell Model Test Method”.

[0072] (2) Test principle: LPS induces RAW264.7 macrophages, which is a classic cell model for studying inflammatory factors. By comparing the difference in TNF-α content secreted by RAW264.7 after negative control and test sample administration, the ability of the test sample to inhibit the secretion of cell inflammatory factors is evaluated, and the soothing efficacy of the test sample is explored.

[0073] (3) Test cells: Mouse monocyte macrophage leukemia cell line RAW264.7 was selected. The cell line passed the cell line quality control test and the results were qualified. The test generation number was P20.

[0074] (4) Main reagents: fetal bovine serum, high-sugar DMEM medium, penicillin-streptomycin solution, trypsin-EDTA solution, TNF-α detection kit, lipopolysaccharide, dexamethasone sodium phosphate (DSP).

[0075] (5) Test method

[0076] ① Grouping and control

[0077] Product dose grouping:

[0078] The test sample group is the above prepared composition 1-8 diluted to 1% sample solution with cell culture medium (MTT cytotoxicity test is performed first to determine the safe working concentration).

[0079] Test control information: ① Blank control: complete culture medium; ② Negative control: 0.5 μg / mL LPS stimulation; ③ Positive control: 0.5 μg / mL LPS + 100 μg / mL dexamethasone sodium phosphate (DSP).

[0080] ② Operation steps

[0081] 1) Cell preparation: After stable passage twice from the frozen cell culture, inoculate the 96-well plate and place the 96-well plate in a 5v / v% CO2 incubator overnight.

[0082] 2) Drug administration: Discard the culture medium in the 96-well plate, and carry out drug administration. The blank control group is given complete culture medium, the negative control group is given cell culture medium containing 0.5 μg / mL LPS, and the rest of the groups are given cell culture medium containing 0.5 μg / mL LPS + corresponding concentration of test sample, and 6 replicates are set for each group. After drug administration, the 96-well plate is placed in a CO2 incubator for 24 h.

[0083] 3) Inflammatory factor detection: After incubation, detection is carried out according to the instructions of the TNF-α enzyme-linked immunoassay kit, and the absorbance of each sample well is measured at 450 nm using a microplate reader.

[0084] (6) Experimental results

[0085] Inhibition rate (%) = (A0-A1) / A0x100%

[0086] Inhibition rate (%) = (A0-A1) / A0x100%

[0087] Table 3 TNF-α inflammatory factor content and inhibition rate in macrophages

[0088]

[0089] Note: "a" indicates a significant difference compared with the negative control group, "aa": p<0.01, "aaa": p<0.001; "b" indicates a significant difference compared with sample group 2, "bb": p<0.01, "bbb": p<0.001.

[0090] Under the condition of LPS stimulation culture, the TNF-α inflammatory factor content and inhibition rate of sample groups 1-8 in macrophages are shown in Table 3. Compared with the negative control group, the TNF-α inflammatory factor content of macrophages treated with sample groups 1-3 containing Huangjing fermentation broth, Paeonia extract and long-stem grape caulerpa extract was significantly reduced, and the TNF-α inhibition rates were 38.26%, 41.46% and 40.29% respectively, indicating that the compound plant extracts 1-3 had significant ability to inhibit the secretion of TNF-α inflammatory factor. Compared with sample group 2, the anti-inflammatory performance of sample groups 4-8 decreased significantly, among which sample groups 4 and 5 had obvious difference in inhibition rate, indicating that Huangjing fermentation broth, Paeonia extract and long-stem grape caulerpa extract played a key role in the formula, and the absence of any component led to a significant decrease in performance; the replacement of Huangjing fermentation broth 1 with Huangjing water extract, Huangjing fermentation broth 2 and Huangjing fermentation broth 3 in sample groups 6-8 led to a certain degree of decrease in anti-inflammatory performance, indicating that the active ingredients in the fermentation broth increased after the compound fermentation of Lactobacillus pentosus and Bacillus subtilis, making the composition have more excellent soothing effect.

[0091] 2. Anti-allergic efficacy test - hyaluronidase inhibition test

[0092] Hyaluronidase is a specific lytic enzyme for hyaluronic acid, which plays an important inhibitory role in many development and regulation processes in the human body, such as cell adhesion, organ formation, wound healing, tumor occurrence and vascular formation. Inhibition of the activity of hyaluronidase can cause hyaluronic acid to be decomposed, thereby maintaining normal physiological functions. Hyaluronidase is a participant in type I allergic reactions. Most allergic reactions are type I allergic reactions related to the activity of hyaluronidase in the body. The inhibition capacity of a sample on hyaluronidase reflects the ability of the sample to inhibit type I allergic reactions. The greater the inhibition rate of hyaluronidase, the stronger the anti-allergic activity.

[0093] 1. Test materials:

[0094] Take compositions 1-8 as the sample solution to be tested; 500 U / mL hyaluronidase solution; pH = 5.4 acetic acid buffer; 0.5 mg / mL sodium hyaluronate solution; acetylacetone solution (3.5 mL acetylacetone is dissolved in 50 mL 0.1 mol / L sodium carbonate solution); P-DAB color developing agent (0.8 g p-dimethylaminobenzaldehyde is uniformly mixed with 15 mL concentrated HCl and anhydrous ethanol); all are prepared immediately before use.

[0095] 2. Test method: The Elson-Morgan method is referred to. In test tube No. 1, 0.5 mL of the sample solution to be tested and 0.5 mL of the hyaluronidase solution are added; in test tube No. 2, 0.5 mL of the sample solution to be tested and 0.5 mL of the acetic acid buffer are added; in test tube No. 3, 0.5 mL of distilled water and 0.5 mL of the hyaluronidase solution are added; in test tube No. 4, 0.5 mL of distilled water and 0.5 mL of the acetic acid buffer are added, and the mixture is incubated at 37°C for 20 min; in test tubes No. 1 and No. 3, 0.5 mL of the sodium hyaluronate solution is added, and in test tubes No. 2 and No. 4, 0.5 mL of the acetic acid buffer is added, and the mixture is incubated at 37°C for 40 min; after being left to stand at room temperature for 10 min, 0.5 mL of the acetylacetone solution, 0.1 mL of the NaOH solution (5 mol / L) and 0.5 mL of distilled water are added, and the mixture is heated in a boiling water bath for 15 min and then immediately cooled with ice water for 10 min; after being left to stand at room temperature for 10 min, 1 mL of the P-DAB color developing agent is added; after being shaken thoroughly, 3.5 mL of anhydrous ethanol is added, and the mixture is left to stand at room temperature for 30 min, thereby developing color; the absorbance A1, A2, A3 and A4 of test tubes No. 1, No. 2, No. 3 and No. 4, respectively, is measured at 530 nm; and the inhibition rate of hyaluronidase is calculated according to the following formula.

[0096] Hyaluronidase inhibition rate = [(A3-A4)-(A1-A2)] / (A3-A4) x 100%

[0097] In the formula: A1, A2, A3, A4 are respectively OD values of solutions in No.1, No.2, No.3, No.4 test tubes.

[0098] 3. Test results:

[0099] The inhibition rate of hyaluronidase is shown in Table 4.

[0100] Table 4 Inhibition rate of hyaluronidase

[0101] Group Hyaluronidase inhibition rate / % Composition 1 61.3 Composition 2 73.4 Composition 3 70.8 Composition 4 42.8 Composition 5 39.6 Composition 6 50.1 Composition 7 52.7 Composition 8 58.2

[0102] According to the test results, the compositions 1-3 have excellent in-vitro hyaluronidase inhibition efficacy, reaching 61.3%, 73.4% and 70.8% respectively. The hyaluronidase inhibition rate of the compositions 4-8 is obviously decreased compared with the composition 2, indicating that the provided rhizoma polygonati fermentation liquor, paeonia extract and apium nodiflorum extract have better soothing, anti-allergy and itching-relieving effects when used in combination.

[0103] Finally, it should be noted that: the above only describes the preferred embodiments of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments or make equivalent replacements to some technical features. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A composition having soothing antipruritic efficacy, characterized in that, By mass percent, comprising the following components: Compound plant extract 13-15%; Polyhydric alcohol 3-8%; Water supplement to 100%; The compound plant extract is compounded by Huangjing fermentation liquor, Peony extract and Caulescent grape extract; The preparation method of the Huangjing fermentation liquor comprises the following steps: Step 1-1. Huangjing is pulverized and sieved to obtain Huangjing powder; Step 1-2. Huangjing powder is mixed with water at a material-liquid ratio of 1: (10-15) g / mL to obtain a fermentation substrate; Step 1-3. Compound bacteria liquor of Lactobacillus pentosus and Bacillus subtilis is taken at an inoculation amount of 8-10 v / v%, added to the fermentation substrate, the fermentation temperature is 30-37℃, the stirring speed is 100-150 r / min, the aeration amount is 0.5-1vvm, and the fermentation culture is carried out for 2-3 days, after the fermentation is completed, sterilization is carried out at 121℃ for 20 min, and centrifugation is carried out after cooling to obtain Huangjing fermentation liquor.

2. The composition for soothing and relieving itching according to claim 1, wherein The viable cell number of Lactobacillus pentosus in the complex bacterial liquid is (1-2) x 10 8 CFU / mL, and the viable cell number of Bacillus subtilis is (5-6) x 10 8 CFU / mL.

3. The composition for soothing and relieving itching according to claim 2, wherein The preparation method of the Peony extract comprises the following steps: Step 2-1. A certain amount of Peony is pulverized and then soaked in water, the soaking time is 12-16h, the soaking temperature is 25-35℃, the material-water ratio is 1: (10-15) g / mL, after the soaking is completed, centrifugal separation is carried out to obtain filtrate 1 and residue; Step 2-2. The residue is added with 70v / v% ethanol aqueous solution, the material-liquid ratio is 1: (20-25) g / mL, extraction is carried out in a 60-70℃ water bath for 120-140min, after the extraction is completed, centrifugal separation is carried out to obtain filtrate 2, the filtrate 2 is combined with the filtrate 1, and drying is carried out under reduced pressure to obtain Peony extract.

4. The composition for soothing and relieving itching according to claim 3, wherein The preparation method of the Caulescent grape extract comprises the following steps: Step 3-1. Dry Caulescent grape is pulverized and sieved to obtain Caulescent grape powder for use; Step 3-2. Caulescent grape powder is mixed with 75v / v% ethanol aqueous solution at a material-liquid ratio of 1: (30-45) g / mL, extraction is carried out in a 60-70℃ water bath for 100-120min, after the extraction is completed, centrifugal separation is carried out, the residue is removed, and drying is carried out under reduced pressure to obtain Caulescent grape extract.

5. The composition for soothing and relieving itching according to claim 4, wherein The compound plant extract is compounded by Huangjing fermentation liquor, Peony extract and Caulescent grape extract at a mass ratio of 10: (1-4) :

1.

6. The composition for soothing and relieving itching according to claim 1, wherein The polyhydric alcohol is at least one of glycerol, 1,3-propanediol and 1,2-hexanediol.

7. Use of the composition with soothing and antipruritic effects according to any one of claims 1-6 in the preparation of a skin care product with soothing, anti-sensitivity and / or repairing effects.

8. A skin-resident agent having soothing antipruritic efficacy, characterized in that, The composition with soothing and antipruritic effects according to any one of claims 1-6.

9. The skin resident agent having soothing and antipruritic efficacy according to claim 8, wherein Further comprising components: humectants, emollients, preservatives, pH adjusters, emulsifiers, solvents.

10. The skin resident agent having soothing and antipruritic efficacy according to claim 9, wherein The addition amount of the composition with soothing and antipruritic effects in the skin resident agent is 5-10wt%.

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