EDNA enrichment device capable of being flexibly deployed

Abstract

The invention relates to the technical field of aquatic organism environment detection, and discloses an eDNA enrichment device capable of being flexibly deployed, the eDNA enrichment device comprises a throwing rod, a traction rope and a plurality of enrichment modules, the traction rope is connected with the throwing rod, and the plurality of enrichment modules are arranged on the traction rope at intervals; the enrichment module comprises a supporting mesh bag, a filter membrane, a pre-filtering net, a buoyancy block and a balancing weight, and the filter membrane is arranged in the supporting mesh bag; the pre-filtering net is attached to the supporting net bag; the buoyancy block and the balancing weight are arranged on the supporting mesh bag; the buoyancy block and the balancing weight are oppositely arranged on the two sides of the filter membrane. A plurality of enrichment modules can be accurately put into a target water area without depending on a carrier, and each enrichment module can be suspended and kept stable at a preset water depth, has good structure supporting performance, can effectively support a filter membrane to be unfolded and keep the filter membrane in a flat state, realizes synchronous sampling of a plurality of water layers, and is high in practicability. The device is suitable for a strong water flow or wave environment, the enrichment efficiency of eDNA is improved, the working efficiency is improved, and the labor cost is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of aquatic organism detection, in particular to a flexible eDNA enrichment device. BACKGROUND

[0002] Environmental DNA (eDNA) technology is a new type of biological monitoring method that has emerged in recent years with the development of genomics and high-throughput sequencing technology. This technology collects, extracts and analyzes the DNA released by target organisms in the environment (such as water, soil or sediments), thereby achieving detection and evaluation of biological species and their distribution. Compared with traditional survey methods such as netting, visual observation or electric trapping, eDNA technology has significant advantages such as non-invasiveness, ease of operation, low learning cost and high sensitivity. Currently, this technology has been widely used in biological diversity investigation and ecological monitoring of aquatic ecosystems, and has gradually developed into one of the mainstream methods in the field of aquatic organism detection.

[0003] In the prior art, efficient enrichment and preservation of eDNA samples are key links to ensure the accuracy of detection results. The methods currently in use include active sampling and passive sampling. Active sampling is a method that uses external pressure to make water pass through a filter membrane, thereby trapping DNA on the surface of the filter membrane. The sampling device is usually composed of a water pump, a power supply and a pipeline, and has poor overall portability, often relying on a ship for transportation, or the collected water samples need to be transported back to the laboratory for filtration. The sampling process is complicated and labor-intensive. Passive sampling is a technology that relies on natural forces such as water flow and waves to enrich eDNA, without the need for external pressure devices, and has the advantages of simple structure and no need for power supply.

[0004] However, the existing enrichment modules have the defects of lack of structural support, insufficient flexibility in remote deployment and depth adjustment, and are difficult to meet the sampling requirements in complex environmental conditions.

[0005] Therefore, the prior art still needs to be improved and developed. SUMMARY

[0006] In view of the shortcomings of the prior art, the present application aims to provide a flexible eDNA enrichment device and an enrichment method thereof, which can solve the problem of the lack of structural support of the existing passive sampling device, which is prone to deformation, resulting in a collection efficiency that cannot meet the detection requirements.

[0007] The technical solution of the present application is as follows: A flexible eDNA enrichment device, comprising a throwing rod, a towing rope and a plurality of enrichment modules, the towing rope being connected to the throwing rod, and the plurality of enrichment modules being arranged at intervals on the towing rope; and the enrichment module comprises: a support net bag; a filter membrane arranged in the support net bag; a pre-filtering net attached to the support net bag; and a buoyancy block and a counterweight block, both arranged on the support net bag, the buoyancy block and the counterweight block being oppositely arranged on two sides of the filter membrane; The enrichment method of the eDNA enrichment device comprises the following steps: determining a target sampling area, wherein the target sampling area is determined according to the geographical position and sampling range of the water body to be sampled; determining the depth data of the water body in the target sampling area, determining the number of enrichment modules based on the depth data, and determining the weight of the buoyancy block and the counterweight block on each enrichment module; Assembling all the enrichment modules and fixing them on the preset position nodes of the towing rope in sequence; Throwing the enrichment modules into the water body in the target sampling area by swinging the throwing rod to carry out enrichment operation, and recording the sampling time synchronously; When the sampling time reaches the preset time length, the enrichment modules are recovered, the filter membrane in the enrichment module is taken out, and the filter membrane is placed in a sterile sealed bag for frozen preservation, and the enrichment operation is ended.

[0008] The flexible eDNA enrichment device, wherein the support net bag comprises a first support net and a second support net arranged oppositely, the first support net is provided with a water inlet hole, and the second support net is provided with a water outlet hole. The edges of the first support net and the second support net are connected by heat sealing, a receiving cavity is formed between the first support net and the second support net, one side of the receiving cavity communicates with the water inlet hole, and the other side of the receiving cavity communicates with the water outlet hole, and the filter membrane is arranged in the receiving cavity, and the pre-filtering net is arranged on the first support net.

[0009] The flexible eDNA enrichment device, wherein the first support net, the second support net and the pre-filtering net are all square nets with equal side lengths, and the edges of the pre-filtering net are connected with the first support net by heat sealing.

[0010] The flexible eDNA enrichment device, wherein the support net bag comprises a top edge and a bottom edge arranged oppositely. The buoyancy block comprises a foam strip extending along the top edge, and the counterweight block comprises at least two lead blocks, and the at least two lead blocks are arranged on the bottom edge in a spaced manner.

[0011] The flexible deployable eDNA enrichment device, wherein the first supporting mesh, the second supporting mesh and the pre-filtering mesh have a side length of 60 mm; the filter membrane has a circular shape and a diameter of 47 mm.

[0012] The flexible deployable eDNA enrichment device, wherein the traction rope comprises: a main rope connected with the throwing rod; a plurality of sub-ropes connected with the main rope; a plurality of hooks connected with the sub-ropes and used for hanging the supporting mesh bag; wherein four sub-ropes form a group of connecting ropes in a conical shape, and each group of connecting ropes is used for connecting one enrichment module.

[0013] The flexible deployable eDNA enrichment device, wherein the throwing rod comprises: a telescopic rod body; a winding hub arranged on the telescopic rod body and used for winding the traction rope; a guide ring arranged on the telescopic rod body and used for pulling the traction rope.

[0014] The flexible deployable eDNA enrichment device, wherein the telescopic rod body is an aluminum alloy rod body, the length of the telescopic rod body is 3-8 m, the diameter of the traction rope is 0.4-0.8 mm, and the length of the traction rope is 5-50 m.

[0015] The flexible deployable eDNA enrichment device, wherein the eDNA enrichment device comprises a foldable support, and at least two clamping positions are arranged on the foldable support, and the clamping positions are used for clamping the telescopic rod body.

[0016] The flexible deployable eDNA enrichment device, wherein the supporting mesh bag is a polyethylene plastic mesh bag, and the pore size of the supporting mesh bag is 8-10 mm; the filter membrane is a glass fiber filter membrane, and the pore size of the filter membrane is 0.3-0.7 microns; the pre-filtering mesh is a nylon mesh, and the pore size of the pre-filtering mesh is 100-200 microns.

[0017] Compared with the prior art, the embodiments of the present application have the following advantages: The eDNA enrichment device disclosed by the application can realize the synchronous sampling of multiple water layers within a certain range from the shore, and has a simple structure and is convenient for a single person to simultaneously control multiple devices. First, the target sampling area is determined, then the number of enrichment modules is determined according to the water depth, and the multiple enrichment modules are bundled and fixed to the traction rope, the inertia of the rotating throwing rod is used to drive the traction rope to be thrown to the target sampling area, then the enrichment modules automatically sink to the preset water depth under the action of the gravity of the counterweight, and sampling is performed. When the water flows, the silt is filtered out through the pre-filtering net, then enters the supporting net bag, and passes through the filter membrane to realize the enrichment of eDNA. The vertical upward tension is provided by the buoyancy block, and the vertical downward tension is provided by the counterweight, so that the supporting net bag is suspended in the water body in a vertical state and remains in a vertical state, so that the filter membrane between the buoyancy block and the counterweight can remain flat, reduce deformation, wrinkles, twisting, folding and the like caused by water flow impact, increase the effective filtering area, improve the enrichment efficiency, and enhance the environmental adaptability of the device, which is beneficial to meet the use requirements in the complex water body environment in the field. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments described in the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0019] Figure 1 The application scenario diagram of the eDNA enrichment device which can be flexibly deployed in an embodiment of the present application; Figure 2 The flowchart of the eDNA enrichment method in an embodiment of the present application; Figure 3 The structural schematic diagram of the eDNA enrichment device which can be flexibly deployed in an embodiment of the present application; Figure 4 The longitudinal sectional view of the enrichment module in an embodiment of the present application; Figure 5 The front view of the enrichment module in an embodiment of the present application; Figure 6 The structural explosion diagram of the enrichment module in an embodiment of the present application.

[0020] Wherein, 10, throwing pole; 11, telescopic pole body; 12, winding wheel hub; 13, guide ring; 20, traction rope; 21, main rope; 22, sub rope; 23, hook; 30, enrichment module; 31, support net bag; 311, first support mesh; 3111, water inlet hole; 312, second support mesh; 3121, water outlet hole; 313, storage cavity; 32, filter membrane; 33, pre-filtering mesh; 34, buoyancy block; 35, counterweight block; 40, foldable support; 41, support rod. DETAILED DESCRIPTION

[0021] In order to enable persons skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by persons skilled in the art without creative labor fall within the protection scope of the present application.

[0022] Due to manufacturing techniques and / or tolerances, variations in the shapes illustrated in the drawings can occur. Therefore, the examples described herein are not limited to the specific shapes illustrated in the drawings, but include variations in shapes that occur during manufacturing. The flow diagrams illustrated in the drawings are examples only and are not necessarily required to include all of the activities and operations or steps, or the order of the activities and operations or steps must be followed. For example, some activities or steps can be eliminated, combined, or partially combined, and thus the order of the actual execution can be changed according to the actual situation.

[0023] As used herein, the term "and / or" includes any one of the listed related items and any combination of any two or more of the listed related items.

[0024] Although terms such as "first", "second", and "third" can be used herein to describe various components, assemblies, regions, layers or parts, these components, assemblies, regions, layers or parts are not limited by these terms. Rather, these terms are used only to distinguish one component, assembly, region, layer or part from another component, assembly, region, layer or part. Therefore, the first component, assembly, region, layer or part described in the examples described herein can also be referred to as the second component, assembly, region, layer or part without departing from the teachings of the examples.

[0025] For ease of description, spatially relative terms, such as "on", "upper", "lower", "above", and "below", can be used herein for the purpose of illustrating one element's relationship to another element within the figures. Such spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as being "on" or "upper" relative to other elements would then be oriented "below" or "lower" relative to the other elements. Thus, the term "on" encompasses both an "on" and "below" orientation. The device can be otherwise oriented (e.g., rotated 90 degrees or at other orientations) and the spatially relative terms used herein interpreted accordingly.

[0026] The terminology used herein is for the purpose of describing various examples only and is not intended to be limiting of the disclosure. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. The terms "comprises", "comprising", "includes", "including" and "has", "having" and the like are inclusive and are intended to be equivalent to the term "consisting of".

[0027] Referring to Figure 1 , Figure 3 , Figure 4 and Figure 5 , in one embodiment of the present application, a flexible deployment eDNA enrichment device is disclosed, which comprises a throwing pole 10, a traction rope 20 and a plurality of enrichment modules 30, the traction rope 20 is connected with the throwing pole 10, and a plurality of the enrichment modules 30 are arranged on the traction rope 20 at intervals; and the enrichment module 30 comprises a supporting net bag 31, a filter membrane 32, a pre-filtering net 33, a buoyancy block 34 and a counterweight block 35, the filter membrane 32 is arranged in the supporting net bag 31; the pre-filtering net 33 is attached to the supporting net bag 31; the buoyancy block 34 and the counterweight block 35 are both arranged on the supporting net bag 31; and the buoyancy block 34 and the counterweight block 35 are oppositely arranged on both sides of the filter membrane 32.

[0028] The eDNA enrichment device disclosed in the embodiment is used in environmental biological detection, and is used for biological diversity monitoring by extracting biological genetic material from environmental samples (mainly water bodies). Specifically, it can be used in the field environment, and sample collection is realized by passing through the filter membrane 32 by the natural flowing water in the field. The structure is simple, and power supply is not required, so it is convenient to carry and easy to operate, and has a wide application prospect in the field of environmental science.

[0029] Specifically, the eDNA enrichment device disclosed in the embodiment is thrown to the target sampling area by the inertia of the turning of the throwing rod 10, and then the enrichment module 30 automatically sinks to the preset water depth under the gravity of the counterweight 35 to perform sampling.

[0030] The enrichment module 30 disclosed in the embodiment is immersed in the water body. When the water body flows, the silt is filtered out through the pre-filtering screen 33, and then enters the support net bag 31 and passes through the filter membrane 32 to realize the enrichment of eDNA. In the actual sampling process, the water body is free to flow, and sudden impact force may be generated, so the filter membrane 32 is easily pushed.

[0031] In the embodiment, the vertical upward tension is provided by the buoyancy block 34, and the vertical downward tension is provided by the counterweight 35, so that the support net bag 31 is suspended in the water body in a vertical state and is kept in the vertical state. The filter membrane 32 is between the buoyancy block 34 and the counterweight 35, and the two sides are close to the inner wall of the support net bag 31, so that the filter membrane 32 can be kept flat, the deformation, wrinkle, twist, folding and the like caused by the impact of the water flow are reduced, the effective filtering area is increased, the enrichment efficiency is improved, the environmental adaptability of the device is enhanced, and the use demand in the complex water body environment in the field is met.

[0032] As shown in Figure 2 The eDNA enrichment method disclosed in the application is used for the flexible eDNA enrichment device as any one of the above, and comprises the following steps. In step S10, the target sampling area is determined. The target sampling area is determined according to the geographical position and the sampling range of the water body to be sampled.

[0033] According to the actual work requirement, the condition of the water body can be determined by a sonar, a camera and the like, and the target sampling area to be detected is drawn, and the distance between the sampling area and the shore can be detected by a range finder and the like, and then the angle and the force of the throwing are calculated.

[0034] In step S20, the depth data of the water body in the target sampling area is determined, the number of enrichment modules 30 is determined based on the depth data, and the weight of the buoyancy block 34 and the counterweight 35 on each enrichment module 30 is determined.

[0035] Based on the depth of the target sampling area, the number of enrichment modules 30 required in the sampling process is calculated, the target suspension depth of each enrichment module 30 is calculated, and further, the weight of the buoyancy block 34 and the counterweight 35 carried on the corresponding enrichment module 30 is calculated.

[0036] In step S30, all the enrichment modules 30 are assembled and fixed on the preset position nodes of the traction rope 20 in sequence.

[0037] Based on the calculation results, the appropriate weight of the buoyancy block 34 and the counterweight block 35 are selected, the plurality of enrichment modules 30 are completely assembled, and are fixed to the traction rope 20, so as to be thrown into the sampling area for sampling at one time.

[0038] In step S40, the enrichment module 30 is thrown into the water body of the target sampling area by swinging the throwing rod 10 to carry out the enrichment operation, and the sampling time is recorded synchronously.

[0039] The sampling time is recorded synchronously, which can improve the control dimension of the detection process and reduce the influence of time factors on the sampling result.

[0040] In step S50, when the sampling time reaches the preset length of time, the enrichment module 30 is recovered, the filter membrane 32 in the enrichment module 30 is taken out, and is placed in a sterile sealed bag for frozen preservation, and the enrichment operation is ended.

[0041] According to the flow rate of the water body and the temperature of the water body in the sampling environment and other factors, the time required for sampling can be preset, that is, the predetermined sampling time length, such as 1 minute, 3 minutes or 5 minutes, etc. After the predetermined sampling time length is reached, the enrichment module 30 is recovered, the filter membrane 32 is taken out, and is frozen in a sterile bag, the field enrichment operation is ended, the sample is properly stored, and the sample is conveniently transported back to the laboratory for detection.

[0042] In summary, the eDNA enrichment device disclosed in the embodiment can complete the enrichment of biological genetic material in a specific water area or water layer, is simple to operate, can batch sample, has high deployment efficiency, is beneficial to saving manpower, and improves the practicality and application range of the eDNA passive sampling device.

[0043] As shown in Figure 1 As another embodiment of the present application, the eDNA enrichment device is disclosed, which comprises a foldable support 40, and at least two clamping positions are arranged on the foldable support 40, and the clamping positions are used for clamping the telescopic rod body 11. In the detection process, the throwing rod 10 can be handheld or fixed on the foldable support 40. Specifically, when throwing, the throwing rod 10 can be handheld to control the throwing angle and intensity; in the detection process, the throwing rod 10 can be fixed on the foldable support 40 to keep stable, so as to improve the stability in the detection process, which is beneficial to accurately collecting the sample and saving manpower.

[0044] Specifically, the foldable support 40 disclosed in the embodiment is composed of a plurality of support rods 41, the bottom end of the foldable support 40 can be fixed on the ground, for example, fixed on the ground by riveting with fixing nails. The height adjustment can be realized by adjusting the inclination angle of the support rod 41. Moreover, the top of the foldable support 40 is provided with at least two clamping positions, and the telescopic rod body 11 can be clamped into the clamping position to realize the assembly of the throwing rod 10; in addition, according to the sampling quantity during work, a plurality of throwing rods 10 can be fixed on the foldable support 40 at the same time, so as to be placed in a centralized manner, facilitating storage and further saving manpower. The foldable support 40 has simple structure, high flexibility in use and strong adaptability in the field environment, which is conducive to further improving the sampling efficiency.

[0045] As shown in Figure 3 As another embodiment of the present application, the throwing rod 10 is disclosed, which comprises a telescopic rod body 11, a winding hub 12 and a guide ring 13, the winding hub 12 is arranged on the telescopic rod body 11; the winding hub 12 is used for winding the traction; the guide ring 13 is arranged on the telescopic rod body 11 and used for pulling the traction rope 20.

[0046] The telescopic rod body 11 disclosed in the embodiment is an aluminum alloy rod body, which has high structural strength and is not easy to deform; the telescopic rod body 11 can be a multi-stage sleeve rod, the total length of the telescopic rod body 11 is 3-8 meters, the length of each section is 50 centimeters, the length is 50 centimeters when being contracted to the shortest state, which is convenient for storage; when being stretched to the longest state, it can be stretched into deep water area. During detection, the distance between the target area and the shore can be adjusted flexibly, further increasing the flexibility of the device in use.

[0047] Specifically, the winding hub 12 and the guide ring 13 are arranged on the telescopic rod body 11, so as to recover the traction rope 20 along the axial direction of the rod body, avoid dragging the enrichment module 30 in the water body and prevent damage.

[0048] As shown in Figure 3 As another embodiment of the present application, the traction rope 20 is disclosed, which comprises a main rope 21, a plurality of sub-ropes 22 and a plurality of hooks 23, the main rope 21 is connected with the throwing rod 10; a plurality of the sub-ropes 22 are connected with the main rope 21; the hooks 23 are connected with the sub-ropes 22 and used for hanging the support net bag 31; wherein, every four sub-ropes 22 form a group of tapered connecting ropes, and each group of connecting ropes is used for connecting one enrichment module 30.

[0049] The eDNA enrichment device disclosed in the embodiment can throw the enrichment module 30 into the water body by setting the throwing rod 10 and the traction rope 20, and can be directly lifted when recovered. Compared with the traditional mode of transportation by means of a ship, a drone or a diver, the operation risk is reduced and the cost is saved. Therefore, the eDNA enrichment device disclosed in the embodiment is more flexible and simple to operate, is more suitable for operation in the field environment, and is conducive to popularization and application in the production monitoring field.

[0050] Specifically, the throwing rod 10 is similar to the structure of a fishing rod, the main rope 21 of the traction rope 20 is bound on the throwing rod 10, the main rope 21 is connected with at least four sub-ropes 22 at the end, and the sub-ropes 22 are connected with the support net bag 31 through the hooks 23. When working, the detection personnel can hold the throwing rod 10 and stand on the shore, extend the throwing rod 10 to the target position, and then lower the main rope 21 to make the support net bag 31 contact with the water body and slowly sink to the predetermined depth. At this time, every four sub-ropes 22 form a connecting rope group, and each connecting rope group provides a pulling force to one support net bag 31 to balance the lateral thrust generated by the water body on the enrichment module 30. The buoyancy block 34 and the counterweight block 35 maintain the vertical state of the support net bag 31, so that the support net bag 31 can maintain a stable state and suspend in the water body to continuously collect samples.

[0051] As shown in Figure 5 In the embodiment, the hooks 23 can be made of stainless steel, four hooks 23 are arranged on the four corners of the support net bag 31 respectively, and the four sub-ropes 22 are equal in length and connected at the same position of the main rope 21. The connecting rope groups are arranged in a conical shape to balance the pulling force in each direction, so that the stress distribution on the support net bag 31 is more balanced, the inclination probability is reduced, and the enrichment efficiency of the filter membrane 32 is further improved.

[0052] Specifically, in another embodiment of the embodiment, the sub-ropes 22 are provided with 8, 16 or 32 sub-ropes, and the support net bag 31 is provided with 2, 3 or 4 support net bags. In the embodiment, multiple enrichment modules 30 can be connected on the main rope 21 to simultaneously use multiple filter membranes 32 for sampling, thereby improving the detection efficiency; and multiple water layers in the water body can be sampled to increase the comprehensiveness of detection.

[0053] In the embodiment, the diameter of the main rope 21 is 0.4-0.8 mm, and the length is 5-50 m. By setting the high-strength main rope 21, multiple support net bags 31 can be carried, and the main rope 21 can be repeatedly thrown to increase the use efficiency. At the same time, the main rope 21 is long, and the throwing distance is farther, which is conducive to accurately delivering the filter membrane 32 to the center of the water body.

[0054] As shown in Figure 4 and Figure 6As another embodiment of the present application, it is shown that the support net bag 31 comprises a first support net 311 and a second support net 312 arranged oppositely, the first support net 311 is provided with an inlet hole 3111, and the second support net 312 is provided with an outlet hole 3121; the edges of the first support net 311 and the second support net 312 are connected by heat sealing; a receiving cavity 313 is formed between the first support net 311 and the second support net 312, one side of the receiving cavity 313 communicates with the inlet hole 3111, and the other side communicates with the outlet hole 3121; and the filter membrane 32 is arranged in the receiving cavity 313; and the pre-filtering net 33 is arranged on the first support net 311.

[0055] The first support net 311 and the second support net 312 disclosed in the embodiment can be cut from the same kind of plastic net. During manufacturing, the filter membrane 32 is first placed between the first support net 311 and the second support net 312, the first support net 311 and the second support net 312 are aligned, and the edges are heated to realize edge sealing connection by heat sealing technology, so as to manufacture a complete support net bag 31. At this time, a complete receiving cavity 313 is formed between the first support net 311 and the second support net 312, the filter membrane 32 is encapsulated in the receiving cavity 313, and is tightly attached to the first support net 311 and the second support net 312, so that the supporting effect is enhanced, and the filter membrane 32 is kept flat and will not be distorted. On the other hand, by abutting the first support net 311 and the second support net 312 against both sides of the filter membrane 32, the supporting effect can be enhanced, the filter membrane 32 is less likely to be torn, and the impact resistance of the device is improved.

[0056] Specifically, the water body flows into the receiving cavity 313 from the inlet hole 3111, and flows out of the outlet hole 3121 after passing through the filter membrane 32, and the sample containing biological genetic material information is intercepted in the filter membrane 32. The pre-filtering layer is arranged on the first support net 311, so that the water body first passes through the pre-filtering layer and then flows into the receiving cavity 313. The pre-filtering layer can intercept impurities, so as to avoid that the impurities such as silt in the water body block the inlet hole 3111 or the filter membrane 32, and further improve the effective use efficiency of the filter membrane 32.

[0057] For example Figure 6 As another embodiment of the present application, it is shown that the first support net 311, the second support net 312 and the pre-filtering net 33 are all square nets, and the side lengths are equal; and the edge of the pre-filtering net 33 is connected with the first support net 311 by heat sealing.

[0058] The support net bag 31 and the pre-filtering net 33 disclosed in the embodiment can be made of high-strength plastic material. The plastic material has the advantages of light weight, good corrosion resistance, low deformation, low manufacturing cost and easy processing. Through extrusion molding or mold molding, a plurality of square meshes of equal size are formed to be connected integrally through heat sealing, thereby increasing the structural stability of the device, and no other fixing parts are needed, so the structure can be simplified and the device is convenient to use in the field.

[0059] Specifically, the square structure has high symmetry, so the support net bag 31 is arranged in a square shape in the embodiment, which is beneficial to keep stable and reduce the situation of turning on one side or tilting on one side of the whole support net bag 31 in the water body, thereby keeping the stability of the support net bag 31 and the filter membrane 32, further improving the stability in the sampling process, and improving the sampling efficiency.

[0060] Meanwhile, the pre-filtering net 33 is used to filter the water sample entering the support net bag 31 in the embodiment, so the pre-filtering net 33 is manufactured according to the size of the first support mesh 311 to completely cover the whole surface of the first support mesh 311, thereby ensuring the pre-filtering effect. On the other hand, the pre-filtering net 33 has the same size as the first support mesh 311, which is also beneficial to align the edges and then perform heat sealing.

[0061] It should be noted that the shape of the support net bag 31 and the shape of the pre-filtering net 33 disclosed in the embodiment are only examples and not exhaustive. For example, equilateral triangle, regular pentagon, circle and other highly symmetrical shapes can be used as the shape of the support net bag 31 and the pre-filtering net 33, as long as the technical effects disclosed in the application are achieved, and these alternatives should be within the scope of protection of the application as equivalent alternatives of the inventive concept.

[0062] Specifically, in another embodiment of the embodiment, two pre-filtering nets 33 are arranged, one pre-filtering net 33 is arranged outside the first support mesh 311, and the other pre-filtering net 33 is arranged outside the second support mesh 312. In the field operation environment, the flow of the water body cannot be predicted, and the water flow direction may change due to factors such as terrain, rainfall and strong wind. Therefore, the pre-filtering net 33 is arranged on both sides of the support net bag 31, which can further protect the filter membrane 32 and prevent the filter membrane 32 from being polluted by the silt entering the support net bag 31. Of course, after the pre-filtering nets 33 are arranged on both sides, the water body can be introduced into both sides of the support net bag 31, which can further improve the sampling speed.

[0063] Specifically, as another embodiment of the application, the support net bag 31 includes oppositely arranged top and bottom edges; the buoyancy block 34 includes a foam strip, the foam strip extends along the top edge; the counterweight block 35 includes at least two lead blocks, and at least two lead blocks are arranged on the bottom edge.

[0064] The foam strip is used as the buoyant block 34 and the lead block is used as the counterweight block 35 in this embodiment, which are only examples and not exhaustive, and other types of buoyant block 34 and counterweight block 35 that can achieve the technical effects disclosed in this application and are equivalent replacements of the inventive concept should also be within the protection scope of this application.

[0065] The foam strip is arranged along the top edge to generate uniform and vertical upward buoyancy on the entire top edge. When two lead blocks are arranged, they are arranged at the two corners of the lower end of the support mesh bag 31. When three lead blocks are arranged, they are arranged at the two corners and the midpoint of the lower end of the support mesh bag 31. In this way, multiple lead blocks are uniformly distributed on the bottom edge to generate vertical downward tension on the entire bottom edge.

[0066] Therefore, the support mesh bag 31 is pulled by a pair of opposite forces, keeping it in a taut state and improving stability, so that it can withstand greater impact force and adapt to more complex water flow environments.

[0067] Specifically, during actual operation, the volume of the foam strip and the mass of the lead block can be adjusted according to the depth of the target sampling position to balance the tension at both ends of the support mesh bag 31, so that the filter membrane 32 can be suspended in the water body and kept at a specific depth for sampling, thereby improving the effectiveness of sampling.

[0068] Specifically, as another embodiment of the present application, the edge length of the first support mesh 311, the second support mesh 312, and the pre-filtering mesh 33 is 60 mm; the shape of the filter membrane 32 is circular, and the diameter of the filter membrane 32 is 47 mm.

[0069] The eDNA enrichment device disclosed in this embodiment is used in field environmental detection work, and the edge length of the first support mesh 311 and the second support mesh 312 is 60 mm, which is convenient to carry. Moreover, after heat sealing the edges, a square receiving cavity 313 with an edge length of about 50-55 mm can be formed, and a circular filter membrane 32 with a diameter of 47 mm is arranged in the receiving cavity 313, which can fully utilize the space of the receiving cavity 313, fully collect samples, and maintain the mobility of the filter membrane 32, so that the sample can be quickly taken out when the support mesh bag 31 is disassembled.

[0070] It should be noted that the size of the support mesh bag 31 and the size of the filter membrane 32 disclosed in this embodiment are set according to actual work requirements, and other choices can be made in different working environments as long as they can achieve the technical effects disclosed in this application and are equivalent replacements of the inventive concept, which should also be within the protection scope of this application.

[0071] Specifically, as another embodiment of the present application, the support net bag 31 is a polyethylene plastic net bag, the aperture of the support net bag 31 is 8-10 mm; the filter membrane 32 is a glass fiber filter membrane 32, the aperture of the filter membrane 32 is 0.3-0.7 microns; and the pre-filtering net 33 is a nylon net, the aperture of the pre-filtering net 33 is 100-200 microns.

[0072] In the embodiment, the filter membrane 32 is made of glass fiber material and has an aperture of 0.3-0.7 microns, so as to intercept the tiny biological genetic material in the water body. The support net bag 31 is made of high-strength polyethylene material and has an aperture of 8-10 mm, so as to quickly pass the water sample. The pre-filtering net 33 is made of nylon material and has a mesh structure, and has the advantages of wear resistance, corrosion resistance and impact resistance, so as to protect the support net bag 31 and the filter membrane 32. The pre-filtering net 33 has an aperture of 100-200 microns, so as to filter out the large particles such as silt, dry branches and leaves in the water body, so as to avoid the large particles from contacting the filter membrane 32 and avoid blocking the filter membrane 32.

[0073] In summary, the present application discloses a flexible eDNA enrichment device, which comprises a throwing rod 10, a traction rope 20 and a plurality of enrichment modules 30. The traction rope 20 is connected to the throwing rod 10, and the enrichment modules 30 are arranged on the traction rope 20 at intervals. The enrichment module 30 comprises a support net bag 31, a filter membrane 32, a pre-filtering net 33, a buoyancy block 34 and a counterweight block 35. The filter membrane 32 is arranged in the support net bag 31. The pre-filtering net 33 is attached to the support net bag 31. The buoyancy block 34 and the counterweight block 35 are arranged on the support net bag 31. The buoyancy block 34 and the counterweight block 35 are arranged on the two sides of the filter membrane 32. The eDNA enrichment device disclosed in the embodiment uses the inertia of the throwing rod 10 to drive the traction rope 20 to be thrown to the target sampling area, and then the enrichment module 30 automatically sinks to the preset water depth under the action of the gravity of the counterweight block 35 to perform sampling. During the sampling process, the support net bag 31 is in a vertical state and is suspended, and the filter membrane 32 is kept flat, so as to increase the effective filtering area and improve the enrichment efficiency, thereby enhancing the environmental adaptability of the device and facilitating the use requirements in the complex water environment in the field.

[0074] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict.

[0075] It should be noted that the present application introduces the specific structure and working principle of the flexible eDNA enrichment device and the enrichment method thereof, but the application of the present application is not limited to the flexible eDNA enrichment device and the enrichment method thereof, and can also be applied to the production and use of other similar workpieces.

[0076] It should be understood that the application is not limited to the precise construction that has been described above and shown in the accompanying drawings, and that various modifications and changes can be made by those skilled in the art without departing from the scope of the application. The scope of the application should only be limited by the claims appended hereto.

[0077] The above description is merely the preferred embodiment of this application, and is not intended to limit the scope of the application. Any modification, equivalent replacement or improvement made without departing from the spirit and principle of the application should be included in the protection scope of the application.

Claims

1. A flexible deployable eDNA enrichment device, characterized in that, The eDNA enrichment device comprises a throwing pole, a traction rope connected with the throwing pole, and a plurality of enrichment modules arranged on the traction rope at intervals. A support net bag; A filter membrane arranged in the support net bag; A pre-filtering net attached to the support net bag; and A buoyancy block and a counterweight block, both arranged on the support net bag, with the buoyancy block and the counterweight block arranged on opposite sides of the filter membrane. The enrichment method of the eDNA enrichment device comprises: Determining a target sampling area, wherein the target sampling area is determined according to the geographical position and sampling range of the water body to be sampled; Determining the depth data of the water body in the target sampling area, determining the number of enrichment modules based on the depth data, and determining the weight of the buoyancy block and the counterweight block on each enrichment module; Assembling all the enrichment modules and fixing them on the traction rope at preset position nodes in sequence; Throwing the enrichment modules into the water body in the target sampling area by swinging the throwing pole to carry out enrichment operation, and recording the sampling time synchronously; When the sampling time reaches the preset time length, the enrichment modules are recovered, the filter membrane in the enrichment modules is taken out and placed in a sterile sealed bag for frozen preservation, and the enrichment operation is completed.

2. The flexibly deployable eDNA enrichment device of claim 1, wherein, The support net bag comprises a first support mesh and a second support mesh arranged oppositely, the first support mesh is provided with a water inlet hole, and the second support mesh is provided with a water outlet hole; The edges of the first support mesh and the second support mesh are connected by heat sealing; a receiving cavity is formed between the first support mesh and the second support mesh, one side of the receiving cavity communicates with the water inlet hole, and the other side communicates with the water outlet hole; and the filter membrane is arranged in the receiving cavity; the pre-filtering net is arranged on the first support mesh.

3. The flexibly deployable eDNA enrichment device of claim 2, wherein, The first support mesh, the second support mesh and the pre-filtering net are all square meshes with equal side lengths; the edges of the pre-filtering net are connected with the first support mesh by heat sealing.

4. The flexibly deployable eDNA enrichment device of claim 3, wherein, The support net bag comprises a top edge and a bottom edge arranged oppositely; The buoyancy block comprises a foam strip extending along the top edge; the counterweight block comprises at least two lead blocks arranged on the bottom edge at intervals.

5. The flexibly deployable eDNA enrichment device of claim 3, wherein, The side length of the first support mesh, the second support mesh and the pre-filtering net is 60 mm; the shape of the filter membrane is circular, and the diameter of the filter membrane is 47 mm.

6. The flexibly deployable eDNA enrichment device of claim 1, wherein, The traction rope comprises: A main rope connected with the throwing pole; A plurality of sub-ropes connected with the main rope; A plurality of hooks connected with the sub-ropes for hanging the support net bag; Each four sub-ropes form a group of tapered connecting ropes, and each group of connecting ropes is used to connect one enrichment module.

7. The flexibly deployable eDNA enrichment device of claim 1, wherein, The throwing pole comprises: A telescopic pole body; A winding hub arranged on the telescopic pole body, used to wind the traction rope; A guide ring arranged on the telescopic pole body, used to pull the traction rope.

8. The flexibly deployable eDNA enrichment device of claim 7, wherein, The telescopic rod body is an aluminum alloy rod body, the length of the telescopic rod body is 3-8 meters; the diameter of the traction rope is 0.4-0.8 millimeters, and the length of the traction rope is 5-50 meters.

9. The flexibly deployable eDNA enrichment device of claim 7, wherein, The eDNA enrichment device comprises a foldable support, and at least two clamping positions are arranged on the foldable support, and the clamping positions are used for clamping the telescopic rod body.

10. The flexibly deployable eDNA enrichment device according to any one of claims 1 to 9, wherein, The support net bag is a polyethylene plastic net bag, and the pore size of the support net bag is 8-10 millimeters; The filter membrane is a glass fiber filter membrane, and the pore size of the filter membrane is 0.3-0.7 microns; The pre-filtering net is a nylon net, and the pore size of the pre-filtering net is 100-200 microns.

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