96-hole micro-volume analysis plate

Abstract

The invention provides a 96-well micro-volume analysis plate, and relates to the technical field of 96-well plates. The 96-hole micro-volume analysis plate comprises an outer frame body, a bottom plate, a panel, a groove row plate, a connecting plate, sample adding holes and the like. Trapezoidal holes are formed in the panel, sliding grooves are formed in the groove row plate, the connecting plate slides in the sliding grooves, conical sample adding holes are fixed to the top of the connecting plate, and the sample adding holes penetrate through the trapezoidal holes and are in one-to-one correspondence; a limiting ring is arranged on the periphery of the sample adding hole, a rectangular frame in a through groove in the bottom of the bottom plate is connected with a connecting plate and a push plate, the push plate drives the connecting plate to ascend and descend through a trapezoidal protruding block and a stepped connecting part and is matched with a bayonet and a protruding point to fix the position, and reinforcing ribs are arranged at the top of the groove arrangement plate. According to the structure, orderly stretching and retracting of the sample adding holes are achieved, the sample adding aiming area is enlarged, hole site confusion is avoided, the rigidity of the hole plate is enhanced, the problems that manual sample adding is prone to errors and automatic sample adding is prone to deviation are solved, and operation efficiency and accuracy are improved.

Description

Technical Field

[0001] This invention relates to the field of 96-well plate technology, specifically a 96-well microvolume analysis plate. Background Technology

[0002] 96-well plates, as standard laboratory consumables in biomedical research and drug development, are characterized by an 8-row × 12-column well arrangement and are widely used in scenarios such as analytical platform establishment and candidate drug screening. Compared to 384-well plates, 96-well plates are more advantageous for small-volume sample analysis because they are compatible with most manual and electric pipettes, are easier to operate, and can reduce reagent waste in small-sample experiments.

[0003] The existing 96-well plate has obvious shortcomings in the sample loading operation: when loading manually, the dense well positions can easily cause visual confusion and increase the risk of loading the wrong well; when loading automatically, the movement or positioning of the robotic arm can easily cause slight deformation of the well plate, resulting in the misalignment of the loading needle with the well position.

[0004] To address this, we developed a new 96-well microvolume analysis plate. Summary of the Invention

[0005] (a) Technical problems to be solved To address the shortcomings of existing technologies, this invention provides a 96-well microvolume analysis plate. By designing a connecting plate lifting structure that can be driven by a push plate, and in conjunction with components such as conical sample dispensing holes and reinforcing ribs, it achieves the orderly expansion and contraction of sample dispensing holes and structural reinforcement functions. This solves the problems of easy confusion due to densely packed well positions when manually dispensing samples on existing 96-well plates, and sample dispensing deviation caused by easy deformation of the well plate when automatically dispensing samples.

[0006] (II) Technical Solution To achieve the above objectives, the present invention is implemented through the following technical solution: a 96-well micro-volume analysis plate, an outer frame, a bottom plate fixedly installed at the bottom of the inner frame, a panel fixedly installed at the top of the inner frame, and multiple sets of trapezoidal holes are linearly arrayed through the panel. The bottom plate is fixedly connected to the top of the groove plate. The groove plate has several sliding grooves in a linear array. A connecting plate is slidably fitted inside the sliding groove. Several sample holes are fixedly installed in a linear array on the top of the connecting plate. The sample holes are conical. The top of the sample hole passes through a trapezoidal hole at the corresponding position. Each sample hole has a one-to-one correspondence with a single trapezoidal hole.

[0007] Preferably, a limiting ring is fixedly connected to the peripheral side of the sample feeding hole, and the limiting ring is adapted to the trapezoidal hole.

[0008] Preferably, the bottom of the base plate has a plurality of through slots arranged in a linear array, the through slots being connected to the sliding grooves, and each through slot having a one-to-one correspondence with a single sliding groove.

[0009] Preferably, a rectangular frame body is slidably connected inside the through slot, and the rectangular frame body is fixedly connected to the bottom of the connecting plate, and the opposite two inner sides of the rectangular frame body are symmetrically provided with an engaging chamfer at both ends.

[0010] Preferably, the outer frame body and the bottom plate on the same side are slidably connected with a push plate, the push plate is fixedly connected with a trapezoidal protrusion at the top, the push plate is fixedly connected with a stepped connection portion at the bottom, and the stepped connection portion and the trapezoidal protrusion are arranged in a head-tail connection manner along the length direction of the push plate.

[0011] Preferably, a plurality of rectangular frame bodies are slidably connected with the push plate.

[0012] Preferably, a plurality of clamping holes are fixedly connected to one side of the push plate in a linear array, and a protrusion is formed on an inner side wall of the outer frame body, and the protrusion is matched with the clamping hole.

[0013] Preferably, a plurality of reinforcing ribs are fixedly connected to the top of the groove row plate, and the reinforcing ribs are arranged between the inner side wall of the outer frame body and the sample addition hole and between adjacent sample addition holes.

[0014] (Three) beneficial effects The present application provides a 96-well micro-volume analysis plate, which has the following beneficial effects: 1. When manually adding samples, the 96-well micro-volume analysis plate drives the connecting plate and the sample addition hole to move up and down in order, so that the end of the sample addition hole to be added moves up and away from the panel, and the increased port diameter of the conical sample addition hole significantly expands the aiming area of the sample addition operation. Meanwhile, the design of adding samples row by row avoids visual confusion caused by dense hole positions, greatly reduces the risk of adding wrong holes, and improves the convenience and accuracy of manual sample addition.

[0015] 2. The 96-well micro-volume analysis plate uses the reinforcing ribs on the top of the groove row plate to form a reticular reinforcing structure, which enhances the rigidity and anti-deformation ability of the hole plate as a whole, and reduces the slight deformation of the hole plate during the movement or positioning of the mechanical arm. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 The structure of the present application is shown in the figure; Figure 2 The structure of the present application is shown in the figure; Figure 3 The structure of the present application is shown in the figure; Figure 4 The structure of the present application is shown in the figure; Figure 5 The structure of the present application is shown in the figure; Figure 6Fig. 3 is a schematic view of the structure of the inverted plate, rectangular frame and sample hole group; Figure 7 Fig. 4 is an enlarged view of A in Fig. 3. Figure 3 Fig. 5 is an enlarged view of A in Fig. 3.

[0017] Wherein, 1, outer frame; 2, bottom plate; 3, panel; 4, trapezoidal hole; 5, groove plate; 6, sliding groove; 7, connecting plate; 8, sample hole; 9, limiting ring; 10, through groove; 11, rectangular frame; 12, connecting chamfer; 13, push plate; 14, trapezoidal protrusion; 15, stepped connection; 16, bayonet; 17, protrusion; 18, reinforcing rib. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.

[0019] Embodiments, such as Figure 1 - Figure 7 As shown in Fig. 4, the embodiment of the present application provides a 96-well micro-volume analysis plate, which comprises an outer frame 1, a bottom plate 2 fixedly installed at the inner bottom of the outer frame 1, and a panel 3 fixedly installed at the inner top of the outer frame 1. A plurality of groups of trapezoidal holes 4 are linearly arrayed and through-holed on the panel 3. A groove plate 5 is fixedly connected to the top of the bottom plate 2. A plurality of sliding grooves 6 are linearly arrayed and formed on the groove plate 5. A connecting plate 7 is slidably fitted in the sliding grooves 6. A plurality of sample holes 8 are linearly arrayed and fixedly installed on the top of the connecting plate 7. The sample holes 8 are conical. The top end of each sample hole 8 penetrates through the corresponding trapezoidal hole 4. Each sample hole 8 forms a one-to-one correspondence with a single trapezoidal hole 4. The groove structure of the sliding groove 6 rigidly constrains the moving track of the connecting plate 7, so that the connecting plate 7 can only slide in the vertical direction (up and down) of the sliding groove 6, avoiding horizontal deviation or tilting of the connecting plate 7 during movement, thereby ensuring the accuracy of the lifting of the sample hole 8. The sample hole 8 is designed to be conical, with the top end opening diameter being larger than the bottom, which can effectively expand the aiming area of the sample operation, solving the problem of small and easy-to-mistake sample holes of the hole plate. At the same time, the sample hole 8 corresponds one-to-one with the trapezoidal hole 4 and penetrates through the trapezoidal hole 4 at the top end. The inner wall of the trapezoidal hole 4 can guide the lifting process of the sample hole 8, preventing the sample hole 8 from shaking and ensuring the accuracy of the sample position.

[0020] Specifically, the limiting ring 9 is fixedly connected to the peripheral side of the sample hole 8 and is adapted to the trapezoidal hole 4. When the connecting plate 7 is not moved upward (normal state), the limiting ring 9 is located in the trapezoidal hole 4 and is in contact with the step surface, thereby limiting the sample hole 8 downward and preventing the sample hole 8 from moving downward excessively due to its own gravity or external force. A plurality of through grooves 10 are linearly arranged on the bottom of the bottom plate 2, and the through grooves 10 are in communication with the sliding grooves 6, and each through groove 10 forms a one-to-one correspondence with a single sliding groove 6. A rectangular frame body 11 is slidably fitted in the through groove 10, and the rectangular frame body 11 is fixedly connected to the bottom of the connecting plate 7. The opposite inner sides of the rectangular frame body 11 are symmetrically provided with an engaging chamfer 12 at both ends. The outer frame 1 and the bottom plate 2 on the same side are slidably fitted with a push plate 13, and the top of the push plate 13 is fixedly connected with a trapezoidal protrusion 14. The bottom of the push plate 13 is fixedly connected with a stepped connecting portion 15, and the stepped connecting portion 15 and the trapezoidal protrusion 14 are arranged in a head-to-tail connection along the length direction of the push plate 13. The through groove 10 is in communication with the sliding groove 6, which provides a channel for the rectangular frame body 11 to extend from the bottom of the bottom plate 2 to the inside of the sliding groove 6, so that the rectangular frame body 11 can be fixedly connected to the bottom of the connecting plate 7 through the bottom plate 2, becoming an intermediate part connecting the push plate 13 and the connecting plate 7. The push plate 13 can slide horizontally at the through part of the outer frame 1 and the bottom plate 2, and the trapezoidal protrusion 14 at the top and the stepped connecting portion 15 at the bottom are arranged in a head-to-tail connection, forming a continuous power transmission structure. When the push plate 13 moves, the trapezoidal protrusion 14 or the stepped connecting portion 15 will contact the engaging chamfer 12 of the rectangular frame body 11, and the inclined surface of the engaging chamfer 12 can decompose the horizontal thrust of the push plate 13 into vertical upward or downward components. For example, when the inclined surface of the trapezoidal protrusion 14 contacts the engaging chamfer 12, the horizontal thrust is converted into an upward force, which pushes the rectangular frame body 11 to move the connecting plate 7 upward, so that the limiting ring 9 of a row of sample addition holes 8 on the connecting plate 7 is separated from the stepped surface of the trapezoidal hole 4, and the upward movement of the sample addition hole 8 port facilitates the sample addition operation; as the push plate 13 continues to move, the trapezoidal protrusion 14 is separated from the rectangular frame body 11, and the stepped connecting portion 15 is subsequently in contact with the other engaging chamfer 12 of the rectangular frame body 11, which drives the connecting plate 7 to move downward, so that the limiting ring 9 reenters the trapezoidal hole 4, and the sample addition hole 8 is retracted; at the same time, the continuous movement of the push plate 13 will make the trapezoidal protrusion 14 contact the next rectangular frame body 11, and the above process is repeated. This design realizes the orderly lifting of multiple rows of sample addition holes 8 and the state alternation of the limiting ring 9, and the operator can add samples row by row to avoid confusion of hole positions and improve operation efficiency; The plurality of rectangular frame bodies 11 are slidably fitted with the push plate 13, so that the horizontal movement of the push plate 13 can act on all the rectangular frame bodies 11 synchronously, ensuring that the power of the push plate 13 can be transmitted to each rectangular frame body 11 in turn. This through sliding fit ensures that there is no relative deviation between the push plate 13 and the rectangular frame body 11, and the movement trajectory of the push plate 13 can be accurately transmitted to each rectangular frame body 11, so that multiple connecting plates 7 move up and down in a predetermined order, thereby driving the limiting ring 9 to orderly separate or enter the trapezoidal hole 4, ensuring that the sample addition hole 8 moves up and down regularly, and avoiding structural jamming or operation errors caused by uneven power transmission.

[0021] The push plate 13 is linearly arrayed on one side and is fixedly connected with a plurality of sockets 16, and the inner side wall of the outer frame body 1 is provided with a protrusion 17, which is matched with the socket 16; when the push plate 13 moves to a certain position (such as the limiting ring 9 of a certain row of sample addition holes 8 is separated from the trapezoidal hole 4, and is in a position facilitating sample addition), the protrusion 17 of the inner side wall of the outer frame body 1 will be embedded in the corresponding socket 16 of the push plate 13, and the position of the push plate 13 is fixed by friction and mechanical clamping. This design prevents the push plate 13 from moving accidentally due to external force (such as accidental touch by the operator or equipment vibration) during sample addition, ensures that the row of sample addition holes 8 remains in the port exposure state, avoids the sudden downward movement of the sample addition hole 8, and causes the interruption of sample addition, and guarantees the stability and accuracy of the sample addition operation.

[0022] The top of the groove row plate 5 is fixedly connected with a plurality of reinforcing ribs 18, and the reinforcing ribs 18 are respectively arranged between the inner side wall of the outer frame body 1 and the sample addition hole 8 and between adjacent sample addition holes 8, so that the reinforcing ribs 18 connect the groove row plate 5 with the outer frame body 1, the sample addition hole 8 and the adjacent sample addition hole 8, form a reticular reinforcing structure, can disperse the external force (such as the clamping force of the mechanical arm and the impact force during movement) received by the hole plate, and enhance the rigidity and anti-deformation ability of the overall structure; at the same time, the reinforcing ribs 18 reduce the relative displacement between adjacent sample addition holes 8.

[0023] Working principle: based on the overall frame outer frame body 1, support is formed through the fixedly installed bottom plate 2 and the panel 3, the trapezoidal hole 4 of the panel 3 is matched with the sliding groove 6 of the groove row plate 5, and precise lifting guidance is provided for the connecting plate 7 and the top conical sample addition hole 8; when the 96-hole plate is shipped, the push plate 13 is completely located inside the hole plate, the stepped connection part 15 is in contact with all the rectangular frames 11, the trapezoidal protrusion 14 is not in contact with any one of the rectangular frames 11, and all the sample addition holes 8 are located in the trapezoidal hole 4; during sample addition, the push plate 13 is pulled outward, the rectangular frame 11 connects the connecting plate 7 and the push plate 13 through the through slot 10 of the bottom plate 2, the trapezoidal protrusion 14 and the stepped connection part 15 of the push plate 13 act on the engagement chamfer 12 of the rectangular frame 11, the horizontal pulling force is converted into the vertical lifting force of the connecting plate 7, the orderly stretching and contraction of the multiple rows of sample addition holes 8 are realized, the precise power transmission is guaranteed by the penetrating sliding cooperation of the rectangular frame 11 and the push plate 13, the position of the push plate 13 is fixed by the clamping of the socket 16 of the push plate 13 and the protrusion 17 of the outer frame body 1 to stabilize the sample addition state, and the reticular reinforcing structure formed by the reinforcing ribs 18 at the top of the groove row plate 5 enhances the overall rigidity and reduces the displacement between the holes, so as to finally realize the design goal of convenient, accurate and stable structure for sample addition operation.

[0024] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A 96-well microvolume assay plate comprising an outer frame (1), characterized in that: The outer frame (1) is fixedly installed with a bottom plate (2) at the bottom, and is fixedly installed with a panel (3) at the top, and a plurality of trapezoidal holes (4) are linearly arranged and penetrated on the panel (3); The bottom plate (2) is fixedly connected with a groove plate (5) at the top, a plurality of sliding grooves (6) are linearly arranged on the groove plate (5), a connecting plate (7) is slidably connected in the sliding groove (6), a plurality of sample adding holes (8) are linearly arranged and fixedly installed on the top of the connecting plate (7), the sample adding hole (8) is tapered, the top end of the sample adding hole (8) penetrates the corresponding trapezoidal hole (4), and each sample adding hole (8) forms a one-to-one correspondence with a single trapezoidal hole (4).

2. A 96-well microvolume assay plate according to claim 1, wherein: The limiting ring (9) is fixedly connected to the side surface of the sample adding hole (8), and the limiting ring (9) is matched with the trapezoidal hole (4).

3. A 96-well microvolume assay plate according to claim 2, wherein: A plurality of through grooves (10) are linearly arranged and penetrated on the bottom plate (2), the through groove (10) is communicated with the sliding groove (6), and each through groove (10) forms a one-to-one correspondence with a single sliding groove (6).

4. A 96-well microvolume assay plate according to claim 3, wherein: The rectangular frame (11) is slidably connected in the through groove (10), the rectangular frame (11) is fixedly connected to the bottom of the connecting plate (7), and the rectangular frame (11) is symmetrically provided with an adapter chamfer (12) at both ends of the opposite inner sides.

5. A 96-well microvolume assay plate according to claim 4, wherein: The outer frame (1) and the bottom plate (2) are slidably connected with a push plate (13) on the same side, the push plate (13) is fixedly connected with a trapezoidal protrusion (14) at the top, the push plate (13) is fixedly connected with a stepped connection part (15) at the bottom, and the stepped connection part (15) and the trapezoidal protrusion (14) are arranged in series at the ends along the length direction of the push plate (13).

6. A 96-well microvolume assay plate according to claim 5, wherein: A plurality of rectangular frames (11) are slidably connected with the push plate (13).

7. A 96-well microvolume assay plate according to claim 6, wherein: A plurality of clamping holes (16) are linearly arranged and fixedly connected on one side of the push plate (13), a convex point (17) is arranged on an inner side wall of the outer frame (1), and the convex point (17) is matched with the clamping hole (16).

8. A 96-well microvolume assay plate according to claim 7, wherein: A plurality of reinforcing ribs (18) are fixedly connected to the top of the groove plate (5), and the reinforcing ribs (18) are arranged between the inner side wall of the outer frame (1) and the sample adding hole (8), and between adjacent sample adding holes (8).

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