Fruit and vegetable cleaning method based on micro-nano bubble synergistic cleaning agent

Abstract

The invention discloses a fruit and vegetable cleaning method based on a micro-nano bubble synergistic cleaning agent. The fruit and vegetable cleaning method based on the micro-nano bubble synergistic cleaning agent comprises the following steps that fruits and vegetables are soaked in a micro-nano bubble cleaning agent water solution to be cleaned, and fruit and vegetable cleaning is achieved. According to the cleaning method for reducing the number of microorganisms on the fruits and vegetables, the removal and killing effects on the microorganisms on the surfaces of the fruits and vegetables are greatly enhanced through the synergistic effect of the micro-nano bubbles and the bactericide.

Description

Technical Field

[0001] This invention belongs to the field of fruit and vegetable cleaning technology, and relates to a fruit and vegetable cleaning method based on micro-nano bubble synergistic cleaning agent. Background Technology

[0002] Microbial contamination is a prominent issue during fruit and vegetable processing and storage, primarily involving pathogenic bacteria, fungi, and viruses. These contaminants not only cause spoilage and shorten shelf life but can also act as vectors for foodborne illnesses, posing a potential threat to consumer health. Therefore, developing efficient fruit and vegetable cleaning technologies to remove microorganisms from their surfaces has become a key focus for the industry.

[0003] Micro-nano bubbles (MNBs), as a novel physical cleaning technology, refer to nano-sized gaseous cavities with a diameter of less than 1 μm dispersed in a solution. They mainly exist in two forms: bubbles attached to surfaces and spherical bubbles freely suspended in aqueous solutions. Upon bubble bursting, hydroxyl radicals are generated, disrupting the structure of microorganisms and achieving a sterilization effect. Simultaneously, no harmful substances are produced during the sterilization process, which is beneficial to sustainable development. However, although micro-nano bubble technology has shown broad application prospects in theoretical research and laboratory conditions, it still has certain limitations in practical applications. Especially when facing complex fruit and vegetable surface structures and high concentrations of microbial contamination, relying solely on micro-nano bubble technology often fails to achieve ideal cleaning results, limiting its widespread application in large-scale industrial fruit and vegetable cleaning.

[0004] Therefore, it is necessary to develop a method for cleaning fruits and vegetables based on micro-nano bubbles. Summary of the Invention

[0005] The purpose of this invention is to provide a fruit and vegetable cleaning method based on micro-nano bubble synergistic cleaning agents. This invention reduces the number of microorganisms on fruits and vegetables by significantly enhancing the removal and elimination of microorganisms on the surface of fruits and vegetables through the synergistic effect of micro-nano bubbles and bactericides.

[0006] The present invention provides a fruit and vegetable cleaning method based on micro-nano bubble synergistic cleaning agent, comprising the following steps: immersing the fruit and vegetables in an aqueous solution of micro-nano bubble cleaning agent for cleaning, thereby achieving fruit and vegetable cleaning.

[0007] In the above-mentioned fruit and vegetable cleaning method, the aqueous solution of the micro-nano bubble cleaning agent is prepared using a micro-nano bubble generator. The cleaning agent in the micro-nano bubble cleaning agent aqueous solution is a sodium hypochlorite aqueous solution.

[0008] In the above-mentioned fruit and vegetable cleaning method, the concentration of the cleaning agent aqueous solution in the micro-nano bubble cleaning agent aqueous solution can be 100~200 mg / L, specifically 150 mg / L.

[0009] In the above-mentioned fruit and vegetable cleaning method, the cleaning time can be 5 to 15 minutes, specifically 5, 10, or 15 minutes. Experiments in the examples show that a short cleaning time (less than 5 minutes) has almost no effect, while a long cleaning time has adverse effects (the cleaning effect decreases rather than increases).

[0010] In this invention, the fruit or vegetable may specifically be a small lime.

[0011] The present invention has the following beneficial effects: Compared with cleaning with micro-nano bubbles alone or cleaning agents alone, this invention uses micro-nano bubble technology in combination with cleaning agents to clean fruits and vegetables. The two produce a significant synergistic effect. By enhancing the permeability of the cleaning agent through micro-nano bubbles, and combining physical cavitation and chemical oxidation, the total number of colonies, molds and yeasts is reduced to a greater extent, achieving efficient sterilization and decontamination, which is conducive to improving the food safety of products and actual production efficiency. Detailed Implementation

[0012] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0013] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.

[0014] Example 1 The inlet and outlet pipes were placed in a 150 mg / L sodium hypochlorite aqueous solution. The micro-nano bubble generator was started, and the pressure of the device was maintained between 40 and 60 MPa by adjusting the air intake. After stabilizing for 5 minutes, micro-nano bubble disinfectant water was obtained. Three unwashed small limes were immersed in the micro-nano bubble disinfectant water and washed for different times (5, 10, 15, and 20 minutes) respectively. They were then placed in sterile bags for later use as the experimental group. Comparative Example 1 Place the inlet and outlet pipes in pure water, start the micro-nano bubble generator, and adjust the air intake to keep the device pressure between 40 and 60 MPa. After stabilizing for 5 minutes, micro-nano bubble water is obtained. Immerse 3 unwashed small limes in the micro-nano bubble water and wash them for different times (5, 10, 15, and 20 minutes) respectively. Then put them into sterile bags for later use as control group 1.

[0015] Comparative Example 2 Three unwashed small limes were immersed in a 150 mg / L sodium hypochlorite solution and washed for different times (5, 10, 15, 20 min) before being placed in sterile bags for later use as control group 2.

[0016] Comparative Example 3 Three unwashed small limes were immersed in pure water and rinsed for different times (5, 10, 15, 20 min) before being placed in sterile bags for later use as control group 3.

[0017] Comparative Example 4 Take 3 unwashed small limes and put them directly into a sterile bag for later use as a blank group.

[0018] Example 2 The small limes in the sterile bags of Example 1 and Comparative Examples 1-4 were transferred to Erlenmeyer flasks containing 225 mL of sterile water. After shaking for 5 min, the first gradient sample dilution was obtained. The total number of colonies, mold and yeast were determined according to GB 4789.2-2022 and GB4789.15-2016. The results are shown in Table 1.

[0019] Table 1. Effects of micro / nanobubbles, cleaning agents, and their synergistic cleaning on the number of microorganisms in lime.

[0020] In Table 1, the cleaning methods are as follows: 1 represents pure water rinsing in Comparative Example 3, 2 represents micro-nano bubble cleaning in Comparative Example 1, 3 represents sodium hypochlorite cleaning in Comparative Example 2, and 4 represents synergistic cleaning of micro-nano bubbles and cleaning agent in Example 1. Different lowercase letters indicate significant differences between different cleaning times. p <0.05).

[0021] The effects of different cleaning times (5, 10, 15, and 20 min) on the cleaning effect were investigated in Examples 1 and 1-3 of this invention. As shown in Table 1, a short cleaning time (less than 5 min) has almost no effect on microbial removal, a cleaning time of 5 min is effective, and a longer cleaning time results in better cleaning. However, a cleaning time of 20 min resulted in a higher number of microorganisms in the limes than a cleaning time of 15 min, indicating that a longer cleaning time is not necessarily better. A longer cleaning time has adverse effects, and the cleaning effect is reduced rather than increased. Therefore, this application prefers a cleaning time of 5 min to 15 min.

[0022] As shown in Table 1, compared with rinsing with pure water in Comparative Example 3, the number of microorganisms on the surface of the small limes was significantly reduced after cleaning with micro-nano bubbles in Comparative Example 1 and sodium hypochlorite cleaning agent in Comparative Example 2, indicating that both micro-nano bubble cleaning and cleaning agent (sodium hypochlorite) cleaning have significant bactericidal effects on the microorganisms on the surface of the small limes. However, when micro-nano bubbles and sodium hypochlorite cleaning agent were used synergistically in Example 1 of this invention, a more significant bactericidal effect was observed. Specifically, based on the individual cleaning of Comparative Examples 1-3, the total number of colonies, mold, and yeast on the surface of the small limes treated by this invention was further reduced by about 1 lg·CFU / g. This invention may enhance the permeability of sodium hypochlorite cleaning agent through micro-nano bubbles, thereby enabling sodium hypochlorite cleaning agent to cause stronger damage to the microbial structure.

Claims

1. A method for cleaning fruits and vegetables based on a micro-nano bubble synergistic cleaning agent, comprising the following steps: immersing the fruits and vegetables in an aqueous solution of the micro-nano bubble cleaning agent for cleaning, thereby achieving fruit and vegetable cleaning.

2. The fruit and vegetable washing method according to claim 1, characterized in that, The micro-nano bubble cleaning agent aqueous solution is prepared using a micro-nano bubble generator; The cleaning agent in the micro-nano bubble cleaning agent aqueous solution is a sodium hypochlorite aqueous solution.

3. The fruit and vegetable washing method according to claim 1 or 2, characterized in that, The concentration of the cleaning agent aqueous solution in the micro-nano bubble cleaning agent is 100~200 mg / L.

4. The fruit and vegetable washing method according to any one of claims 1-3, characterized in that, The cleaning time is 5-15 minutes.

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