Coenzyme Q10 nanoparticles and preparation method thereof
By preparing coenzyme Q10 nanoparticles, the stability and water solubility issues of coenzyme Q10 were solved, achieving high encapsulation efficiency and moisture stability, making it suitable for industrial production.
Patent Information
- Application Number
- CN202511272714.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-08
- Publication Date
- 2025-11-11
AI Technical Summary
Coenzyme Q10 has poor water solubility and is unstable to light and heat, resulting in poor product stability. The soft capsules are also prone to absorbing moisture, which affects their effectiveness.
Coenzyme Q10 nanoparticles were prepared by antisolvent coprecipitation. Coenzyme Q10 was coated with sodium alginate and hydroxypropyl methylcellulose, and then protected by the hydrophobic properties of ethyl cellulose and zein to form nanoparticles with a particle size of 100-400 nm, which prevented agglomeration and improved stability.
It improves the stability and encapsulation efficiency of coenzyme Q10, maintains stable moisture content, and ensures uniform dispersion of nanoparticles, making it suitable for industrial production.
Smart Images
Figure BDA0005585137460000051
Abstract
Description
Technical Field
[0001] This invention relates to the field of health product formulation technology, and in particular to a coenzyme Q10 nanoparticle and its preparation method. Background Technology
[0002] Coenzyme Q10 is widely distributed in the human body, present in various organs, tissues, subcellular components, and plasma. It is a nutrient that activates human cells and cellular energy, possessing functions such as enhancing immunity, anti-oxidation, delaying aging, and increasing vitality. Medically, it is widely used in the treatment of cardiovascular diseases, and both domestically and internationally, it is widely used to provide nutritional foods for immunity and anti-aging. Coenzyme Q10 has poor water solubility and is unstable in light, heat, and alkaline environments, easily oxidizing, which limits the preparation and use of coenzyme Q10 products. Currently, coenzyme Q10 is often made into soft capsules; however, soft capsules are prone to hygroscopicity, making moisture control difficult, and high humidity environments can easily reduce the stability of coenzyme Q10. Summary of the Invention
[0003] Based on the technical problems existing in the background technology, the present invention proposes a coenzyme Q10 nanoparticle and its preparation method. The coenzyme Q10 nanoparticle of the present invention has good encapsulation efficiency and hydrophobicity, can maintain moisture stability and improve its stability; and can avoid nanoparticle agglomeration, so that it can be uniformly dispersed with other components and excipients for use in combination.
[0004] This invention proposes a coenzyme Q10 nanoparticle, the raw materials of which include, by weight: 0.16-0.2 parts coenzyme Q10, 8-10 parts zein, 0.2-0.4 parts ethyl cellulose, 1-2 parts sodium alginate, and 0.2-0.4 parts hydroxypropyl methylcellulose.
[0005] Preferably, the particle size of the coenzyme Q10 nanoparticles is 100-400 nm.
[0006] Preferably, the raw materials also include antioxidants, colorings, and sweeteners.
[0007] Antioxidants and pigments can further enhance the antioxidant and photoresistance properties of coenzyme Q10, and improve its stability.
[0008] Preferably, the weight ratio of zeatin to antioxidant is 8-10:0.01-0.02; the preferred antioxidant may be vitamin E, etc.
[0009] Preferably, the weight ratio of sodium alginate to pigment and sweetener is 1-2:0.03-0.05:0.1-0.2; preferably, the pigment can be Sunset Yellow, etc., and the sweetener can be sucrose, etc.
[0010] The present invention also proposes a method for preparing the above-mentioned coenzyme Q10 nanoparticles, comprising the following steps: dissolving coenzyme Q10, ethyl cellulose, and zein in ethanol to obtain solution A; adding solution A dropwise to a mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose, stirring, and freeze-drying to obtain coenzyme Q10 nanoparticles.
[0011] Preferably, the concentration of zeaxanthin in solution A is 0.01-0.013 g / mL.
[0012] Preferably, in the mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose, the concentration of sodium alginate is 0.0012-0.0025 g / mL.
[0013] Preferably, the pH of the mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose is 3.6-3.8.
[0014] The pH can be adjusted using hydrochloric acid.
[0015] Preferably, the mixture is stirred at a speed of 450-550 rpm for 1-2 hours.
[0016] This invention involves dissolving coenzyme Q10, ethyl cellulose, and zein in ethanol, then adding the solution dropwise to a mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose. Through antisolvent co-precipitation, coenzyme Q10, ethyl cellulose, and zein precipitate, and the ethyl cellulose and zein granules encapsulate coenzyme Q10. Furthermore, in an acidic environment, sodium alginate adsorbs zein granules via electrostatic interactions, further encapsulating coenzyme Q10 with sodium alginate and hydroxypropyl methylcellulose, ultimately yielding coenzyme Q10 nanoparticles with excellent encapsulation efficiency and improved stability. It can maintain a weakly acidic environment to further improve the stability of coenzyme Q10; and the synergistic effect of ethyl cellulose, zein, and hydroxypropyl methylcellulose can give the nanoparticles good hydrophobicity, maintain the moisture stability of the nanoparticles, and further improve the stability of the nanoparticles; in addition, the electrostatic interaction between sodium alginate and zein can make the nanoparticles uniformly dispersed, avoid the agglomeration of nanoparticles, and allow them to be uniformly dispersed with other components (such as phospholipids) and excipients for use in combination; and the preparation process of this invention is simple and the conditions are mild, making it suitable for large-scale industrial production. Detailed Implementation
[0017] The technical solution of the present invention will be described in detail below through specific embodiments. However, it should be clearly stated that these embodiments are only for illustrative purposes and are not intended to limit the scope of the present invention.
[0018] Example 1
[0019] A coenzyme Q10 nanoparticle, the raw materials of which include, by weight: 0.18 parts coenzyme Q10, 9 parts zein, 0.3 parts ethyl cellulose, 1.5 parts sodium alginate, 0.3 parts hydroxypropyl methylcellulose, 0.015 parts vitamin E, 0.04 parts sunset yellow, and 0.15 parts sucrose.
[0020] The preparation method of the above-mentioned coenzyme Q10 nanoparticles includes the following steps: dissolving zein and ethyl cellulose in ethanol, then adding coenzyme Q10 and vitamin E, stirring to dissolve, so that the concentration of zein is 0.011 g / mL, to obtain solution A, which is ready for use.
[0021] Sodium alginate, hydroxypropyl methylcellulose, Sunset Yellow, and sucrose were added to water and stirred to dissolve them, so that the concentration of sodium alginate was 0.0019 g / mL. The pH was adjusted to 3.7 with dilute hydrochloric acid to obtain a mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose for later use.
[0022] Solution A was added dropwise to a mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose while stirring. The mixture was then stirred at 500 rpm for 1.5 h, centrifuged at 3000 rpm for 5 min to remove large particles, and finally freeze-dried at -40℃. The resulting solution was then sieved to obtain coenzyme Q10 nanoparticles. The particle size of the coenzyme Q10 nanoparticles was determined to be 100-400 nm.
[0023] Example 2
[0024] A coenzyme Q10 nanoparticle, the raw materials of which include, by weight: 0.16 parts coenzyme Q10, 8 parts zein, 0.2 parts ethyl cellulose, 2 parts sodium alginate, 0.2 parts hydroxypropyl methylcellulose, 0.01 parts vitamin E, 0.03 parts sunset yellow, and 0.2 parts sucrose.
[0025] The preparation method of the above-mentioned coenzyme Q10 nanoparticles includes the following steps: dissolving zein and ethyl cellulose in ethanol, then adding coenzyme Q10 and vitamin E, stirring to dissolve, so that the concentration of zein is 0.01 g / mL, to obtain solution A, which is ready for use.
[0026] Sodium alginate, hydroxypropyl methylcellulose, Sunset Yellow, and sucrose were added to water and stirred to dissolve them, so that the concentration of sodium alginate was 0.0025 g / mL. The pH was adjusted to 3.6 with dilute hydrochloric acid to obtain a mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose for later use.
[0027] Solution A was added dropwise to a mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose while stirring. The mixture was then stirred at 550 rpm for 1 hour and centrifuged at 3000 rpm for 5 minutes to remove large particles. The solution was then freeze-dried at -40°C and sieved to obtain coenzyme Q10 nanoparticles.
[0028] Example 3
[0029] A coenzyme Q10 nanoparticle, the raw materials of which include, by weight: 0.2 parts coenzyme Q10, 10 parts zein, 0.4 parts ethyl cellulose, 1 part sodium alginate, 0.4 parts hydroxypropyl methylcellulose, 0.02 parts vitamin E, 0.05 parts sunset yellow, and 0.1 parts sucrose.
[0030] The preparation method of the above-mentioned coenzyme Q10 nanoparticles includes the following steps: dissolving zein and ethyl cellulose in ethanol, then adding coenzyme Q10 and vitamin E, stirring to dissolve, so that the concentration of zein is 0.0125 g / mL, to obtain solution A, which is ready for use.
[0031] Sodium alginate, hydroxypropyl methylcellulose, Sunset Yellow, and sucrose were added to water and stirred to dissolve, so that the concentration of sodium alginate was 0.00125 g / mL. The pH was adjusted to 3.8 with dilute hydrochloric acid to obtain a mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose for later use.
[0032] Solution A was added dropwise to a mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose while stirring. The mixture was then stirred at 450 rpm for 2 hours and centrifuged at 3000 rpm for 5 minutes to remove large particles. The solution was then freeze-dried at -40°C and sieved to obtain coenzyme Q10 nanoparticles.
[0033] Comparative Example 1
[0034] A method for preparing coenzyme Q10 nanoparticles includes the following steps:
[0035] Replace the mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose with an aqueous solution, otherwise the same as in Example 1.
[0036] Comparative Example 2
[0037] A method for preparing coenzyme Q10 nanoparticles includes the following steps:
[0038] The aqueous solution of sodium alginate and hydroxypropyl methylcellulose was prepared without sodium alginate, otherwise the same as in Example 1.
[0039] Comparative Example 3
[0040] A method for preparing coenzyme Q10 nanoparticles includes the following steps:
[0041] The aqueous solution of sodium alginate and hydroxypropyl methylcellulose was prepared without hydroxypropyl methylcellulose, and otherwise the same as in Example 1.
[0042] Comparative Example 4
[0043] A method for preparing coenzyme Q10 nanoparticles includes the following steps:
[0044] Solution A does not contain ethyl cellulose, and is otherwise the same as in Example 1.
[0045] The coenzyme Q10 nanoparticles prepared in Examples 1-3 and Comparative Examples 1-4 were used to test their encapsulation efficiency, moisture content, and stability. The results are shown in Table 1.
[0046] Table 1 Test Results
[0047]
[0048] As can be seen from Table 1, the coenzyme Q10 nanoparticles prepared in this invention have good stability, high encapsulation efficiency, and stable moisture content.
[0049] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.
Claims
1. A coenzyme Q10 nanoparticle, characterized in that, Its raw materials, by weight, include: 0.16-0.2 parts coenzyme Q10, 8-10 parts corn gluten, 0.2-0.4 parts ethyl cellulose, 1-2 parts sodium alginate, and 0.2-0.4 parts hydroxypropyl methylcellulose.
2. The coenzyme Q10 nanoparticles according to claim 1, characterized in that, The particle size of coenzyme Q10 nanoparticles is 100-400 nm.
3. The coenzyme Q10 nanoparticles according to claim 1 or 2, characterized in that, Its ingredients also include antioxidants, colorings, and sweeteners.
4. The coenzyme Q10 nanoparticles according to claim 3, characterized in that, The weight ratio of zeatin to antioxidant is 8-10:0.01-0.
02.
5. The coenzyme Q10 nanoparticles according to claim 3, characterized in that, The weight ratio of sodium alginate to coloring and sweetener is 1-2:0.03-0.05:0.1-0.
2.
6. The method for preparing coenzyme Q10 nanoparticles according to any one of claims 1-5, characterized in that, The process includes the following steps: dissolving coenzyme Q10, ethyl cellulose, and zein in ethanol to obtain solution A; adding solution A dropwise to a mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose, stirring, and freeze-drying to obtain coenzyme Q10 nanoparticles.
7. The method for preparing coenzyme Q10 nanoparticles according to claim 6, characterized in that, In solution A, the concentration of zeaxanthin is 0.01-0.013 g / mL.
8. The method for preparing coenzyme Q10 nanoparticles according to claim 6 or 7, characterized in that, In the mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose, the concentration of sodium alginate is 0.0012-0.0025 g / mL.
9. The method for preparing coenzyme Q10 nanoparticles according to any one of claims 6-8, characterized in that, The pH of the mixed aqueous solution of sodium alginate and hydroxypropyl methylcellulose is 3.6-3.
8.
10. The method for preparing coenzyme Q10 nanoparticles according to any one of claims 6-9, characterized in that, Stir at 450-550 rpm for 1-2 hours.