Blueberry special biological organic fertilizer and preparation method thereof

Abstract

The application discloses a kind of blueberry special biological organic fertilizer and preparation method thereof, belong to biological organic fertilizer technical field.The biological organic fertilizer of the application is prepared from grass carbon soil, synergist, pyroligneous liquid, furfural residue, corn straw, phosphoric acid, modifier, microbial inoculant, ferrous sulfate, pine needle, pig manure, EM bacteria liquid.The application is composed of two parts of blueberry special biological organic fertilizer granules and solution.Blueberry special biological organic fertilizer solution is synergized by sodium lignosulfonate and potassium humate in a mass ratio of 1:1 to improve the acidic soil environment around the roots;Activated biochar in blueberry special biological organic fertilizer granules is modified by tea polyphenol and sodium alpha-alkyl sulfonate in a mass ratio of 1:1 to graft strong acidic phosphate functional groups, while the water retention capacity is increased, and various substances such as microbial inoculant, fermented organic nutrients, grass carbon soil and ferrous ion work together to improve blueberry planting soil.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of bio-organic fertilizer, and particularly relates to a special bio-organic fertilizer for blueberries and a preparation method thereof. BACKGROUND

[0002] Blueberry is also called blueberry, belongs to the genus of Vaccinium in the family of Ericaceae, and is a deciduous shrub with unique fruit flavor and rich nutritional value, and is listed as one of the five healthiest foods for human by the Food and Agriculture Organization of the United Nations. Blueberry likes light, and the most suitable pH value of soil is between 4.0 and 4.8. Improper application of chemical fertilizer causes high or low pH value of soil. High pH value of soil leads to iron and magnesium chlorosis of blueberry, and low pH value of soil easily causes manganese poisoning, resulting in death of blueberry. Organic matter in soil can improve soil structure and provide essential nutrients for growth of blueberry. At present, sulfur powder is mostly applied to improve the pH value of soil for blueberry planting. Although the effect can be achieved quickly, sulfur powder is a chemical substance and cannot provide organic matter for soil, and is easy to pollute underground water.

[0003] Patent CN104788177B discloses a special water-retaining bio-organic fertilizer for blueberry, which provides a small environment with loose, breathable, moist and high organic matter content for the root of blueberry by using microorganisms as raw materials, and promotes the absorption of nutrients by the root of blueberry. However, the bio-organic fertilizer has no good effect on the control and improvement of the pH value of soil. The root of blueberry is sensitive to the pH value of soil and requires high pH value of soil. The pH value of soil needs to be considered in the preparation process of the bio-organic fertilizer. Therefore, it is of great significance for the planting of blueberry to prepare a bio-organic fertilizer which can durably and effectively improve the suitable pH value of soil for blueberry planting, provide sufficient organic matter to improve the surrounding environment of soil for blueberry planting, promote the growth of the root of blueberry, improve the stress resistance, increase the water absorption and water retention of the root, and has no pollution. SUMMARY

[0004] The application overcomes the defects of the prior art, discloses a special bio-organic fertilizer for blueberry and a preparation method thereof, and comprises two parts of a special bio-organic fertilizer for blueberry and a solution. The special bio-organic fertilizer solution for blueberry is synergized by wood sulfonate sodium and humic acid potassium at a mass ratio of 1:1 to improve the acidic soil environment around the root. The activated biochar in the special bio-organic fertilizer granules for blueberry is modified by tea polyphenol and alpha-alkyl sulfonate sodium at a mass ratio of 1:1, grafted with strong acidic phosphate functional groups, and the water retention performance is increased. The special bio-organic fertilizer for blueberry synergistically acts with microbial agents, organic nutrients after fermentation, grass charcoal soil and ferrous ions to improve the soil for blueberry planting.

[0005] To achieve the above technical purposes, the technical scheme adopted by the application is as follows.

[0006] The blueberry special biological organic fertilizer is prepared from the following raw materials in parts by weight: grass carbon soil 35-45 parts, synergist 10-15 parts, wood vinegar 50-70 parts, furfural residue 25-30 parts, corn straw 65-90 parts, phosphoric acid 60-80 parts, modifier 3-6 parts, microbial agent 5-8 parts, ferrous sulfate 3-5 parts, pine needle raw material 38-45 parts, pig manure 26-32 parts, and EM bacterial liquid 10-15 parts.

[0007] Preferably, the synergist is sodium lignosulfonate and potassium humate with a mass ratio of 1:1.

[0008] Preferably, the wood vinegar is used after being diluted by 20-35 times.

[0009] Preferably, the phosphoric acid is a phosphoric acid solution with a mass fraction of 15-20%.

[0010] Preferably, the modifier is tea polyphenol and sodium alpha-alkenyl sulfonate with a mass ratio of 1:1.

[0011] Preferably, the microbial agent is fusiform lysine bacillus (Bacillus fusiformis) (Bacillus fusiformis), Bacillus subtilis (Bacillus subtilis), Rhizobium Burkholderia (Burkholderia rhizoxinica), and Pseudomonas stutzeri (Pseudomonas stutzeri) with a mass ratio of 1:1:1:1. Lysinibacillus fusiformis Bacillus subtilis Burkholderia tuberum Pseudomonas stutzeri

[0012] ​​​​More preferably, the said spindly Bacillus licheniformis is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Yihuangyuan 1st Road, Beijing City, China, with the preservation number of CGMCC1.10176 and the original preservation date of July 6, 2009; the said Bacillus subtilis is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Yihuangyuan 1st Road, Beijing City, China, with the preservation number of CGMCC1.15792 and the original preservation date of July 10, 2016; the said Burkholderia nodosa is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Yihuangyuan 1st Road, Beijing City, China, with the preservation number of CGMCC1.10205 and the original preservation date of August 19, 2009; the said Pseudomonas stutzeri is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Yihuangyuan 1st Road, Beijing City, China, with the preservation number of CGMCC1.15316 and the original preservation date of June 15, 2015.

[0013] Each strain used in the present application can be purchased through the preservation center strain catalog inquiry, without the need for repeated biological preservation.

[0014] Preferably, the said pine needle raw material is one or more of pine needle leaves, pine sawdust and pine bark.

[0015] Preferably, the said blueberry special biological organic fertilizer is composed of a blueberry special biological organic fertilizer solution and a granular part.

[0016] A preparation method of a blueberry special biological organic fertilizer, the steps are as follows:

[0017] (1) Dilution of wood vinegar solution for synergistic effect:

[0018] The wood vinegar solution is diluted with water to 25-30 times, and then sodium lignosulfonate and potassium humate are added in sequence, after dissolution, stirring and mixing are uniformly carried out, to obtain a blueberry special biological organic fertilizer solution;

[0019] (2) Preparation of microbial agent:

[0020] The Bacillus licheniformis, Bacillus subtilis, Burkholderia nodosa and Pseudomonas stutzeri are respectively cultured in seed culture medium at 25℃, 30℃, 30℃ and 30℃ for 48 hours, and then inoculated into LB culture medium at a ratio of 5%, and cultured in the LB culture medium until the bacterial concentration reaches OD600≈3.5 to obtain four kinds of bacterial liquid, and then the four kinds of bacterial liquid are mixed uniformly at a mass ratio of 1:1:1:1, and then freeze-dried to obtain a freeze-dried powder, and then a microbial inoculum is obtained;

[0021] (3) Fermentation and decomposition:

[0022] The furfural residue, pine needle raw material and pig manure are respectively dried by ventilation to a water content of ≤5%, crushed to pass through an 80-mesh sieve, and then mixed uniformly, and then added into a fermentation tank, water is added to control the water content to be between 40-60%, and then the EM bacterial liquid is sprayed on the fermentation substrate, and then the fermentation substrate and the EM bacterial liquid are fully contacted by multiple stirring and spraying of the EM bacterial liquid, and then the fermentation is cultured by ventilation for 48 hours, water is added to control the water content to be 65%, and then the mixture is stirred and mixed uniformly, the fermentation tank is sealed with a plastic film, the upper layer is compacted with soil, and then the fermentation is carried out for 6 days, the plastic film is opened, and then the mixture is dried by ventilation until the water content is ≤3%, and then an organic nutrient after fermentation is obtained;

[0023] (4) Preparation of modified activated biochar:

[0024] The corn stalks are washed, crushed to 1-2 cm, and then soaked in a phosphoric acid solution with a mass fraction of 15-20%, and then tea polyphenol and sodium alpha-alkyl sulfonate are added in sequence, and then stirred and mixed uniformly, and then soaked for 8 hours, and then the soaked corn stalks are filtered and taken out, and then added into a reaction kettle, and then nitrogen is introduced to exhaust air, and then the nitrogen flow rate is controlled to be 100 cm3 / min, and then heated, and then the temperature is maintained at 750℃ for 1.5 hours, and then cooled to 25℃, and then crushed to pass through an 80-mesh sieve, and then a modified activated biochar is obtained;

[0025] (5) Preparation of the preparation:

[0026] The turf soil is crushed to pass through an 80-mesh sieve, and then the microbial inoculum prepared in step (2), the fermented organic nutrient prepared in step (3), the modified activated biochar prepared in step (4), and ferrous sulfate are added in sequence, and then stirred and mixed uniformly, and then dried to a water content of ≤15%, and then granulated by a granulator, and then the particles with a particle size of 3-5 mm are screened out, and then a blueberry special biological organic fertilizer granule is obtained.

[0027] A method for applying a blueberry special biological organic fertilizer is provided, and the steps are as follows:

[0028] Before planting blueberries, the soil pH is detected, a 15 cm cave is dug around the root of the blueberry seedling, and the blueberry special biological organic fertilizer prepared in the application is applied according to the soil pH, and then buried as a base fertilizer, and the specific amount is as follows:

[0029] 1-3 year seedlings, the soil pH is between 6.5-7, each blueberry is sequentially applied with 0.2-0.25 Kg of the blueberry special biological organic fertilizer granules prepared in step (5) and 30-50 mL of the blueberry special biological organic fertilizer solution prepared in step (1), and the application amount is adjusted according to the size of the seedlings;

[0030] 1-3 year seedlings, the soil pH is between 6-6.5, each blueberry is sequentially applied with 0.15-0.2 Kg of the blueberry special biological organic fertilizer granules prepared in step (5) and 20-30 mL of the blueberry special biological organic fertilizer solution prepared in step (1), and the application amount is adjusted according to the size of the seedlings;

[0031] 1-3 year seedlings, the soil pH is between 5.5-6, each blueberry is sequentially applied with 0.1-0.15 Kg of the blueberry special biological organic fertilizer granules prepared in step (5) and 10-20 mL of the blueberry special biological organic fertilizer solution prepared in step (1), and the application amount is adjusted according to the size of the seedlings;

[0032] 1-3 year seedlings, the soil pH is between 4.9-5.5, each blueberry is sequentially applied with 0.5-0.1 Kg of the blueberry special biological organic fertilizer granules prepared in step (5) and 10-20 mL of the blueberry special biological organic fertilizer solution prepared in step (1).

[0033] The blueberry special biological organic fertilizer is composed of the blueberry special biological organic fertilizer granules and the solution.

[0034] After the wood vinegar in the blueberry special biological organic fertilizer solution is added with sodium lignosulfonate and potassium humate at a mass ratio of 1:1, the wood vinegar is uniformly dispersed in the soil, adsorbed around the soil around the blueberry roots, maintains the acidity of the blueberry roots, increases the organic matter, improves the absorption and utilization of potassium by crops, and improves the stress resistance of crops.

[0035] In the preparation process of the modified activated carbon of the blueberry special biological organic fertilizer granules, the corn stalks are activated after being soaked with phosphoric acid at a mass fraction of 15-20%, and tea polyphenol and sodium alpha-alkenyl sulfonate at a mass ratio of 1:1 are added to form smaller micropores in the preparation process of the activated biological carbon, so that the phosphate and alpha-alkenyl sulfonate are grafted on the surface of the corn stalk biological carbon, the surface of the biological carbon has strong acidic phosphorus functional groups, and the water retention performance is increased, thereby effectively improving the pH and soil quality of the blueberry planting soil.

[0036] The fusiform lysinibacillus, bacillus subtilis, burkholderia nodosa and pseudomonas stutzeri in the microbial agent play a synergistic role, significantly reduce the pH value of the soil, improve the soil quality, improve the soil fertility of the blueberries, promote the growth and development of the blueberry roots, improve the stress resistance, photosynthetic performance and antioxidant capacity of the blueberries, and significantly improve the fruit quality of the blueberries.

[0037] The fermented pine needle leaves contain acidic substances such as crude tannic acid, which can reduce the pH value of the soil, and the organic matter formed after decomposition can significantly improve the soil compaction, increase the soil permeability, and has the functions of water and fertilizer conservation; the furfural residue and pig manure provide nutrient substances for the rapid propagation of microbial agents, and at the same time provide a large amount of mineral elements and organic matter; the grass charcoal contains fulvic acid, humic acid, humin acid and humin, and these acid ions can absorb hydroxyl ions to reduce the soil pH; the humic acid has strong adsorption capacity, increases the soil aggregate structure, makes the soil loose, and has strong water absorption and nitrogen absorption capacity; the ferrous ion increases the chlorophyll content in the blueberry plant, promotes the occurrence of photosynthesis, and promotes the growth and development of the blueberry plant.

[0038] Advantages

[0039] The blueberry special biological organic fertilizer prepared by the application is composed of two parts of blueberry special biological organic fertilizer granules and solution. The blueberry special biological organic fertilizer solution is synergized by wood sulfonate sodium and humic acid potassium at a mass ratio of 1:1; the blueberry special biological organic fertilizer granules are added with activated biochar modified by tea polyphenol and alpha-alkyl sulfonate sodium at a mass ratio of 1:1, microbial agents, fermented organic nutrients, grass charcoal, ferrous ions, and a plurality of substances synergistically play a role in improving the blueberry planting soil. Compared with the prior art, the wood vinegar acid modified by the synergist has stable pH, enhanced adhesion, and acidic components uniformly adhered around the blueberry roots, which reduces the pH value of the soil and improves the soil organic matter; the added modified activated biochar is added with a modifier during phosphoric acid soaking, which forms more micropores on the surface of the biochar, greatly increases the specific surface area and adsorption capacity of the modified activated biochar, and makes the phosphate and alpha-alkyl sulfonate stablely grafted on the surface of the biochar, which has strong acidic phosphorus functional groups and increases the water retention performance; the added microbial agents cooperate with each other and synergistically play a role to effectively improve the pH value of the acidic soil, promote root growth, and improve the stress resistance of crops; the components in the blueberry special biological organic fertilizer granules and solution synergistically play a role to significantly improve the soil quality of the blueberry planting soil, provide a large amount of organic matter, improve the yield and quality of blueberries, and improve the planting benefits. DETAILED DESCRIPTION

[0040] The technical solutions of the application are further described below in combination with specific examples:

[0041] Example 1

[0042] The blueberry special biological organic fertilizer is prepared from the following raw materials in parts by weight: grass carbon soil 35 parts, synergist 10 parts, wood vinegar 50 parts, furfural residue 25 parts, corn straw 65 parts, phosphoric acid 60 parts, modifier 3 parts, microbial agent 5 parts, ferrous sulfate 3 parts, pine needle raw material 38 parts, pig manure 26 parts and EM bacterial liquid 10 parts.

[0043] The synergist is sodium lignosulfonate and potassium humate at a mass ratio of 1:1.

[0044] The wood vinegar is wood vinegar diluted by 20 times.

[0045] The phosphoric acid is a phosphoric acid solution with a mass fraction of 15%.

[0046] The modifier is tea polyphenol and sodium alpha-alkenyl sulfonate at a mass ratio of 1:1.

[0047] The microbial agent is fusiform lysine bacillus, bacillus subtilis, burkholderia nodosa and pseudomonas stutzeri at a mass ratio of 1:1:1:1.

[0048] The fusiform lysine bacillus is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with a preservation number of CGMCC1.10176; the bacillus subtilis is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with a preservation number of CGMCC1.15792; the burkholderia nodosa is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with a preservation number of CGMCC1.10205; and the pseudomonas stutzeri is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with a preservation number of CGMCC1.15316.

[0049] The pine needle raw material is pine needle.

[0050] The blueberry special biological organic fertilizer is composed of a blueberry special biological organic fertilizer solution and a granule.

[0051] A preparation method of a blueberry special biological organic fertilizer, steps are as follows:

[0052] (1) dilution of wood vinegar for synergistic effect:

[0053] The wood vinegar is diluted with water to 25 times, sodium lignosulfonate and potassium humate are added in sequence, after dissolution, stirring and mixing are uniformly conducted, and a blueberry special biological organic fertilizer solution is obtained;

[0054] (2) preparation of microbial inoculum:

[0055] The fusiform bacillus lysiniformis, bacillus subtilis, burkholderia nodosa and pseudomonas stutzeri are respectively cultured in seed culture medium at temperatures of 25 DEG C, 30 DEG C, 30 DEG C and 30 DEG C for 48 h, and are respectively inoculated into LB culture medium at a 5% inoculation amount, and are cultured in the LB culture medium until the bacterial concentration OD600 is about 3.5 to obtain four kinds of bacterial liquid, and the four kinds of bacterial liquid are mixed uniformly at a mass ratio of 1:1:1:1, and are freeze-dried into freeze-dried powder to obtain a microbial inoculum;

[0056] (3) fermentation and maturation:

[0057] The furfural residue, pine needles and pig manure are respectively ventilated and dried to a water content of less than or equal to 5%, are crushed through an 80-mesh sieve, are stirred and mixed uniformly, are added into a fermentation tank, water is added, the water content is controlled to be between 40%, EM bacterial liquid is sprayed on the fermentation substrate, the fermentation substrate and the EM bacterial liquid are fully contacted by multiple times of stirring and spraying of the EM bacterial liquid, ventilation and fermentation culture are conducted for 48 h, water is added, the water content is controlled to be 65%, stirring and mixing are uniformly conducted, the fermentation tank is sealed with a plastic film, the upper layer is compacted with soil, fermentation is conducted for 6 days, the plastic film is opened, ventilation and drying are conducted until the water content is less than or equal to 3% to obtain fermented organic nutrients;

[0058] (4) preparation of modified activated biochar:

[0059] The corn stalks are washed, are crushed to 1 cm, are soaked in a 15% phosphoric acid solution, tea polyphenol and sodium alpha-alkyl sulfonate are added in sequence, stirring and mixing are uniformly conducted, the soaked corn stalks are filtered and taken out, are added into a reaction kettle, nitrogen is introduced to exhaust air, the nitrogen flow rate is controlled to be 100 cm 3 / min, heating is conducted, the temperature is maintained at 750 DEG C for 1.5 h, the temperature is cooled to 25 DEG C, the crushed corn stalks are passed through an 80-mesh sieve to obtain modified activated biochar;

[0060] (5) preparation:

[0061] The grass charcoal soil is crushed through an 80-mesh screen, and the microbial inoculum prepared in step (2), the fermented organic nutrient prepared in step (3), the modified activated biochar prepared in step (4), and ferrous sulfate are sequentially added and uniformly stirred, and then dried to a water content of less than or equal to 15%, granulated by a granulator, and sieved to obtain granules with a particle size of 3-5 mm, thereby obtaining a blueberry special biological organic fertilizer granule.

[0062] A method for applying the blueberry special biological organic fertilizer is as follows:

[0063] Before planting blueberries, the soil pH is detected, a 15-cm cave is dug around the root of the blueberry seedling, and the blueberry special biological organic fertilizer prepared in the application is applied according to the soil pH as base fertilizer, and the specific amount is as follows:

[0064] For 1-3-year-old seedlings, if the soil pH is between 6.5 and 7, 0.2-0.25 Kg of the blueberry special biological organic fertilizer granule prepared in step (5) and 30-50 mL of the blueberry special biological organic fertilizer solution prepared in step (1) are sequentially applied to each blueberry seedling, and the application amount is adjusted according to the size of the seedling.

[0065] For 1-3-year-old seedlings, if the soil pH is between 6 and 6.5, 0.15-0.2 Kg of the blueberry special biological organic fertilizer granule prepared in step (5) and 20-30 mL of the blueberry special biological organic fertilizer solution prepared in step (1) are sequentially applied to each blueberry seedling, and the application amount is adjusted according to the size of the seedling.

[0066] For 1-3-year-old seedlings, if the soil pH is between 5.5 and 6, 0.1-0.15 Kg of the blueberry special biological organic fertilizer granule prepared in step (5) and 10-20 mL of the blueberry special biological organic fertilizer solution prepared in step (1) are sequentially applied to each blueberry seedling, and the application amount is adjusted according to the size of the seedling.

[0067] For 1-3-year-old seedlings, if the soil pH is between 4.9 and 5.5, 0.5-0.1 Kg of the blueberry special biological organic fertilizer granule prepared in step (5) and 10-20 mL of the blueberry special biological organic fertilizer solution prepared in step (1) are sequentially applied to each blueberry seedling.

[0068] Example 2

[0069] A blueberry special biological organic fertilizer is prepared from the following raw materials by weight: grass charcoal soil 40 parts, synergist 12 parts, wood vinegar 60 parts, furfural residue 28 parts, corn straw 75 parts, phosphoric acid 70 parts, modifier 4 parts, microbial inoculum 6 parts, ferrous sulfate 4 parts, pine needle raw material 42 parts, pig manure 29 parts, and EM bacterial liquid 12 parts.

[0070] The synergist is sodium lignosulfonate and potassium humate in a mass ratio of 1:1.

[0071] The wood vinegar is wood vinegar with a dilution ratio of 30 times.

[0072] The phosphoric acid is a phosphoric acid solution with a mass fraction of 18%.

[0073] The modifier is tea polyphenol and sodium alpha-alkenyl sulfonate with a mass ratio of 1:1.

[0074] The microbial agent is fusiform lysine bacillus, bacillus subtilis, burkholderia nodosa, and pseudomonas stutzeri with a mass ratio of 1:1:1:1.

[0075] The fusiform lysine bacillus is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with a preservation number of CGMCC1.10176; the bacillus subtilis is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with a preservation number of CGMCC1.15792; the burkholderia nodosa is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with a preservation number of CGMCC1.10205; and the pseudomonas stutzeri is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with a preservation number of CGMCC1.15316.

[0076] The pine needle raw material is pine sawdust.

[0077] The blueberry special biological organic fertilizer is composed of a blueberry special biological organic fertilizer solution and a granular part.

[0078] A preparation method of a blueberry special biological organic fertilizer, comprising the following steps:

[0079] (1) Dilution of wood vinegar for synergistic effect:

[0080] The wood vinegar is diluted with water to 28 times, and then sodium lignosulfonate and potassium humate are added in sequence. After dissolution, the mixture is stirred and mixed uniformly to obtain a blueberry special biological organic fertilizer solution.

[0081] (2) Preparation of microbial inoculants:

[0082] Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii were cultured in seed culture media at temperatures of 25℃, 30℃, 30℃, and 30℃ for 48 h, respectively. They were then inoculated into LB medium at an inoculum rate of 5% and cultured in LB medium until the bacterial concentration OD600≈3.5 to obtain four bacterial solutions. The four bacterial solutions were then mixed evenly at a mass ratio of 1:1:1:1 and freeze-dried into freeze-dried powder to obtain the microbial inoculant.

[0083] (3) Fermentation and composting:

[0084] Furfural residue, pine sawdust, and pig manure were dried separately in a ventilated environment until the moisture content was ≤5%. They were then crushed and passed through an 80-mesh sieve, stirred and mixed evenly, and added to a fermentation tank. Water was added to control the moisture content at around 50%. EM bacterial solution was sprayed onto the fermentation substrate. The substrate and EM bacterial solution were stirred and sprayed repeatedly to ensure full contact. The fermentation was carried out in a ventilated environment for 48 hours. Water was added to control the moisture content at 65%. The mixture was stirred and mixed evenly. The fermentation tank was sealed with plastic film, and the top layer was compacted with soil. Fermentation was carried out for 6 days. The plastic film was then opened, and the tank was ventilated and dried until the moisture content was ≤3%, yielding the fermented organic nutrients.

[0085] (4) Preparation of modified activated biochar:

[0086] Wash the corn stalks, crush them to 2cm, and soak them in a 15-20% phosphoric acid solution. Add tea polyphenols and sodium α-alkenyl sulfonate sequentially, stir to mix thoroughly, and soak for 8 hours. Filter and remove the soaked corn stalks, add them to a reaction vessel, and purge the air with nitrogen gas at a flow rate of 100cm. 3 The mixture was heated at 750℃ for 1.5 hours, cooled to 25℃, and pulverized through an 80-mesh sieve to obtain modified activated biochar.

[0087] (5) Formulation:

[0088] The peat soil was crushed and passed through an 80-mesh sieve. The microbial agent prepared in step (2), the fermented organic nutrients prepared in step (3), the modified activated biochar prepared in step (4), and ferrous sulfate were added in sequence. The mixture was stirred and mixed evenly, dried until the moisture content was ≤15%, granulated by a granulator, and granules with a particle size of 3-5mm were screened out to obtain a blueberry-specific bio-organic fertilizer granule.

[0089] The application method of the blueberry-specific bio-organic fertilizer in this embodiment is the same as in Embodiment 1.

[0090] Example 3

[0091] The blueberry special biological organic fertilizer is prepared from the following raw materials in parts by weight: grass carbon soil 45 parts, synergist 15 parts, wood vinegar 70 parts, furfural residue 30 parts, corn straw 90 parts, phosphoric acid 80 parts, modifier 6 parts, microbial agent 8 parts, ferrous sulfate 5 parts, pine needle raw material 45 parts, pig manure 32 parts and EM bacterial liquid 15 parts.

[0092] The synergist is sodium lignosulfonate and potassium humate at a mass ratio of 1:1.

[0093] The wood vinegar is wood vinegar diluted by 35 times.

[0094] The phosphoric acid is a 20% phosphoric acid solution by mass fraction.

[0095] The modifier is tea polyphenol and sodium alpha-alkenyl sulfonate at a mass ratio of 1:1.

[0096] The microbial agent is fusiform lysine bacillus, bacillus subtilis, burkholderia nodosa and pseudomonas stutzeri at a mass ratio of 1:1:1:1.

[0097] The fusiform lysine bacillus is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with the preservation number of CGMCC1.10176; the bacillus subtilis is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with the preservation number of CGMCC1.15792; the burkholderia nodosa is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with the preservation number of CGMCC1.10205; and the pseudomonas stutzeri is purchased from China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Beichen West Road, Chaoyang District, Beijing, with the preservation number of CGMCC1.15316.

[0098] The pine needle raw material is pine bark.

[0099] The blueberry special biological organic fertilizer is composed of a blueberry special biological organic fertilizer solution and a granule.

[0100] A preparation method of a blueberry special biological organic fertilizer, steps are as follows:

[0101] (1) dilution of wood vinegar for synergistic effect:

[0102] The wood vinegar is diluted with water to 30 times, sodium lignosulfonate and potassium humate are added in sequence, after dissolution, stirring and mixing are uniformly conducted, and a blueberry special biological organic fertilizer solution is obtained;

[0103] (2) preparation of microbial agent:

[0104] The fusiform lysine bacillus, bacillus subtilis, burkholderia nodosa and pseudomonas stutzeri are respectively cultured in seed culture medium at temperatures of 25 DEG C, 30 DEG C, 30 DEG C and 30 DEG C for 48 h, and are respectively inoculated into LB culture medium at a inoculation amount of 5%, and are cultured in the LB culture medium until the bacterial concentration OD600 is about 3.5 to obtain four kinds of bacterial liquid, and the four kinds of bacterial liquid are mixed uniformly at a mass ratio of 1:1:1:1, and are freeze-dried into freeze-dried powder to obtain a microbial agent;

[0105] (3) fermentation and maturation:

[0106] The furfural residue, pine bark and pig manure are respectively ventilated and dried to a water content of less than or equal to 5%, are crushed through an 80-mesh sieve, are stirred and mixed uniformly, are added into a fermentation tank, water is added, the water content is controlled to be between 60%, the EM bacterial liquid is sprayed on the fermentation substrate, the fermentation substrate and the EM bacterial liquid are fully contacted by multiple times of stirring and spraying of the EM bacterial liquid, ventilation and fermentation culture are conducted for 48 h, water is added, the water content is controlled to be 65%, stirring and mixing are uniformly conducted, the fermentation tank is sealed with a plastic film, the upper layer is compacted with soil, fermentation is conducted for 6 days, the plastic film is opened, ventilation and drying are conducted until the water content is less than or equal to 3% to obtain fermented organic nutrients;

[0107] (4) preparation of modified activated biochar:

[0108] The corn stalks are washed, are crushed to 2 cm, are soaked in a phosphoric acid solution with a mass fraction of 20%, are stirred and mixed uniformly after tea polyphenol and sodium alpha-alkyl sulfonate are added in sequence, are soaked for 8 h, are filtered and taken out after soaking, are added into a reaction kettle, nitrogen is introduced to exhaust air, the nitrogen flow rate is controlled to be 100 cm 3 / min, heating is conducted, the temperature is maintained at 750 DEG C for 1.5 h, the temperature is cooled to 25 DEG C, and the crushed product is passed through an 80-mesh sieve to obtain modified activated biochar;

[0109] (5) preparation:

[0110] The grass charcoal soil is crushed through an 80-mesh screen, and the microbial inoculant prepared in step (2), the fermented organic nutrient prepared in step (3), the modified activated biochar prepared in step (4), and ferrous sulfate are sequentially added and uniformly mixed, and then dried to a water content of less than or equal to 15%, granulated by a granulator, and sieved to obtain granules with a particle size of 3-5 mm, thereby obtaining a blueberry special biological organic fertilizer granule.

[0111] The application method of the blueberry special biological organic fertilizer of the present embodiment is the same as that of Example 1.

[0112] Comparative Example 1

[0113] The present comparative example is the same as Example 3 except that no synergist is added during the preparation process.

[0114] Comparative Example 2

[0115] The present comparative example is the same as Example 3 except that no sodium lignosulfonate is used as the synergist during the preparation process.

[0116] Comparative Example 3

[0117] The present comparative example is the same as Example 3 except that no potassium humate is used as the synergist during the preparation process.

[0118] Comparative Example 4

[0119] The present comparative example is the same as Example 3 except that no modifier is used during the preparation process.

[0120] Comparative Example 5

[0121] The present comparative example is the same as Example 3 except that no tea polyphenol is used as the modifier during the preparation process.

[0122] Comparative Example 6

[0123] The present comparative example is the same as Example 3 except that no sodium α-alkenyl sulfonate is used as the modifier during the preparation process.

[0124] Comparative Example 7

[0125] The present comparative example is the same as Example 3 except that no fusiform lysinibacillus is used as the microbial inoculant during the preparation process.

[0126] Comparative Example 8

[0127] The present comparative example and example 3 are compared only in the preparation process of the microbial inoculant, Bacillus subtilis is not used in the preparation process, and the rest of the operation process is exactly the same as example 3.

[0128] Comparative example 9

[0129] The present comparative example and example 3 are compared only in the preparation process of the microbial inoculant, Bacillus subtilis is not used in the preparation process, and the rest of the operation process is exactly the same as example 3.

[0130] Comparative example 10

[0131] The present comparative example and example 3 are compared only in the preparation process of the microbial inoculant, Bacillus subtilis is not used in the preparation process, and the rest of the operation process is exactly the same as example 3.

[0132] Field test:

[0133] In October 2016, a field test was conducted in a test field with 28 mu of soil environment similar to the blueberry planting base in Daxing Village, Daxing Town, Linshu County. The average pH of the soil was 5.9, the soil bulk density was 1.1 g / cm 3 , the porosity was 54%, the soil organic matter content was 12.4 g / Kg, and the soil water content was 9.5%. The 1-3 year old seedlings were of the variety Brilliant, and the blueberry seedling tree age and growth were basically the same. Fertilization was carried out one week before the flowering period of blueberries.

[0134] The 28 mu test field was divided into 14 parts, and the blueberry special biological organic fertilizer prepared by examples 1-3 and comparative examples 1-10 of the present application was applied to each group of test fields (the remaining 1 test field was not applied with any fertilizer). In this field test, different blueberry special biological organic fertilizers were applied, and the rest of the operation was exactly the same, which was the conventional blueberry field management method. After the blueberries were harvested, the average fruit weight, single plant yield, yield per mu, blueberry sugar content, and anthocyanin content of each group of blueberries were calculated.

[0135] The specific detection method is as follows:

[0136] Sugar content: detected using a refractometer;

[0137] Anthocyanin: detected according to method GB / T 22244-2008 for determination of proanthocyanidins in health food.

[0138] The specific statistical table is shown in Table 1:

[0139] Table 1: Field test statistical table

[0140]

[0141] According to the statistical results in Table 1, the blueberry fruits are more full after applying the blueberry special biological organic fertilizer prepared by the examples 1-3 of the present application, the sugar content and anthocyanin content are obviously increased, the nutrient content is higher, the yield per mu is more, compared with the blank control group without applying the blueberry special biological organic fertilizer, the average fruit weight of blueberry is 2.6g, increased by 62%; the sugar content is 14.3%, increased by 27%; the anthocyanin content is more than 8mg / g; the yield per mu is 616Kg, increased by 58%, so the components of the blueberry special biological organic fertilizer prepared by the present application play a synergistic role, which can effectively improve the quality and yield of blueberry fruits.

[0142] After the harvest of blueberries is completed, the soil quality of 14 test fields is detected, 6 detection soil samples of 0-20cm near the roots of blueberry plants are randomly collected from each test field by soil drilling, mixed, and 100mg of soil is selected by four division method for detection test, and the pH, bulk density, porosity, organic matter content and water content of the soil are detected.

[0143] The specific detection method is as follows:

[0144] pH: detected by pH meter (Shanghai Leici PHS-2F);

[0145] Bulk density and porosity: the bulk density is determined by ring knife method, combined with soil saturated water content and field water capacity, and the soil porosity is calculated respectively;

[0146] Organic matter: determined by potassium dichromate oxidation-external heating method;

[0147] Water content: determined by drying and weighing method.

[0148] The specific detection results are shown in Table 2:

[0149] Table 2 soil improvement effect table

[0150]

[0151] According to the detection results in Table 2, it can be known that the blueberry special biological organic fertilizer prepared by the examples 1-3 of the present application can effectively improve the soil quality, improve the soil pH, improve the soil bulk density and porosity, increase the organic matter and water content of the root soil, and compared with the blank control, the application of the blueberry special biological organic fertilizer prepared by the examples 1-3 of the present application can reduce the soil pH by 1.1 on average, and the soil pH can be adjusted to the most suitable soil pH for the growth of blueberry plants according to the application method; the loose quality of the soil is obviously improved; the organic matter content of the soil near the root of the blueberry plant is 18.9g / Kg, which is increased by 52%; the soil water content is 14.3%, which is increased by 50%. Therefore, the wood vinegar liquid synergized by the synergist, the active biochar modified by the modifier, and the microbial agent components all play an essential role in the improvement of the blueberry special planting soil, and the components of the present application synergistically play a role, which can effectively improve the soil quality of the blueberry planting soil.

[0152] It should be noted that the above examples are only part of the preferred modes of implementing the present application, not all. Obviously, based on the above examples of the present application, all other examples obtained by those of ordinary skill in the art without creative labor should belong to the scope of protection of the present application.

Claims

1. A special bio-organic fertilizer for blueberries, characterized in that, It is prepared from the following raw materials in parts by weight: 35-45 parts peat moss, 10-15 parts synergist, 50-70 parts wood vinegar, 25-30 parts furfural residue, 65-90 parts corn stalks, 60-80 parts phosphoric acid, 3-6 parts modifier, 5-8 parts microbial agent, 3-5 parts ferrous sulfate, 38-45 parts pine needle raw material, 26-32 parts pig manure, and 10-15 parts EM bacterial solution; the synergist is sodium lignosulfonate and potassium humate in a mass ratio of 1:1; the modifier is tea polyphenols and sodium α-olefin sulfonate in a mass ratio of 1:

1.

2. The blueberry-specific bio-organic fertilizer according to claim 1, characterized in that, The wood vinegar solution should be diluted 20-35 times before use.

3. The blueberry-specific bio-organic fertilizer according to claim 1, characterized in that, The phosphoric acid mentioned is a phosphoric acid solution with a mass fraction of 15-20%.

4. The blueberry-specific bio-organic fertilizer according to claim 1, characterized in that, The microbial agent is Bacillus fusiformis (Lysine-containing Bacillus) in a mass ratio of 1:1:1:

1. Lysinibacillus fusiformis Bacillus subtilis ( Bacillus subtilis Burkholderia lumbricoides ( Burkholderia tuberum ) and Pseudomonas stearothermia ( Pseudomonas stutzeri ).

5. The blueberry-specific bio-organic fertilizer according to claim 4, characterized in that, The preservation number of the *Bacillus fusiformis* is CGMCC1.10176; the preservation number of the *Bacillus subtilis* is CGMCC1.15792; the preservation number of *Burkholderia rumenella* is CGMCC1.10205; and the preservation number of *Pseudomonas schrenckii* is CGMCC1.15316.

6. The blueberry-specific bio-organic fertilizer according to claim 1, characterized in that, The pine needle raw material is one or more of pine needles, pine sawdust, and pine bark.

7. The blueberry-specific bio-organic fertilizer according to claims 1-6, characterized in that, The blueberry-specific bio-organic fertilizer consists of two parts: a blueberry-specific bio-organic fertilizer solution and granules.

8. A method for preparing a blueberry-specific bio-organic fertilizer according to any one of claims 1-6, characterized in that, The preparation steps include the following: (1) Diluting wood vinegar to enhance its effects: Dilute the wood vinegar solution with water to 25-30 times, then add sodium lignosulfonate and potassium humate in sequence. After dissolving, stir and mix evenly to obtain a special bio-organic fertilizer solution for blueberries. (2) Preparation of microbial inoculants: Bacillus fusiformis, Bacillus subtilis, Burkholderia rumenella, and Pseudomonas schrenckii were cultured in seed culture media at temperatures of 25℃, 30℃, 30℃, and 30℃, respectively, for 48 hours. They were then inoculated into LB medium at an inoculum rate of 5% and cultured in LB medium until the bacterial concentration OD600≈3.5 to obtain four bacterial solutions. The four bacterial solutions were then mixed evenly in a mass ratio of 1:1:1:1 and freeze-dried into freeze-dried powder to obtain the microbial inoculant. (3) Fermentation and composting: Furfural residue, pine needles, and pig manure were dried separately with ventilation until the moisture content was ≤5%, crushed and passed through an 80-mesh sieve, mixed evenly, and added to a fermentation tank. Water was added to control the moisture content between 40-60%. EM bacterial solution was sprayed onto the fermentation substrate. The substrate and EM bacterial solution were stirred and sprayed repeatedly to ensure full contact. The fermentation was carried out with ventilation for 48 hours. Water was added to control the moisture content to 65%, and the mixture was stirred evenly. The fermentation tank was sealed with plastic film, and the top layer was compacted with soil. Fermentation was carried out for 6 days. The plastic film was then opened, and the tank was ventilated and dried until the moisture content was ≤3% to obtain the fermented organic nutrients. (4) Preparation of modified activated biochar: Wash the corn stalks, crush them to 1-2 cm, and soak them in a 15-20% phosphoric acid solution. Add tea polyphenols and sodium α-alkenyl sulfonate sequentially, stir to mix thoroughly, and soak for 8 hours. Filter and remove the soaked corn stalks, add them to a reaction vessel, and purge the air with nitrogen gas at a flow rate of 100 cm⁻¹. 3 The mixture was heated at 750℃ for 1.5 hours, cooled to 25℃, and pulverized through an 80-mesh sieve to obtain modified activated biochar. (5) Formulation: The peat soil was crushed and passed through an 80-mesh sieve. The microbial agent prepared in step (2), the fermented organic nutrients prepared in step (3), the modified activated biochar prepared in step (4), and ferrous sulfate were added in sequence. The mixture was stirred and mixed evenly, dried until the moisture content was ≤15%, granulated by a granulator, and granules with a particle size of 3-5mm were screened out to obtain a blueberry-specific bio-organic fertilizer granule.

Images