Stem cell targeted induction detection device for nerve cell repair

Abstract

The invention relates to the technical field of targeted induction detection of stem cells, and discloses a detection device for targeted induction of stem cells for nerve cell repair. Comprising a stem cell detection box body, the interior of the stem cell detection box body is slidably connected with a sliding block, and the side surface of the sliding block is fixedly connected with a culture dish limiting placement seat. According to the stem cell detection device, the stem cell detection probe can be attached to a gap between wiping and cleaning cloth through the arrangement of the locating plates, the locating plates can swing downwards to extrude the first springs under the extrusion influence through continuous movement of the monitoring probe, the locating plates can be separated to locate the stem cell detection probe, and after stem cell detection is completed, the stem cell detection probe can move upwards; the extrusion force borne by the positioning plate is reduced, the wiping cleaning cloth can be attached to the surface of the stem cell detection probe to wipe the stem cell detection probe, and the dryness of the stem cell detection probe is ensured.

Description

Technical Field

[0001] This invention relates to the field of stem cell targeted induction detection technology, specifically a detection device for stem cell targeted induction used for nerve cell repair. Background Technology

[0002] Stem cells are also known as originating cells. Simply put, they are primitive, undifferentiated cells with multi-directional differentiation potential and self-renewal capacity, serving as the original cells that form the various tissues and organs of mammals. Stem cells share common morphological characteristics, typically being round or oval, small in size, with a relatively large nucleus, mostly composed of euchromatin, and exhibiting high telomerase activity. Stem cells can be divided into embryonic stem cells and adult stem cells. The function of stem cell populations is to control and maintain cell regeneration. Generally, between stem cells and their terminally differentiated daughter cells, there exists an intermediate progenitor cell population called "directed progenitor cells," which possess limited proliferative capacity and restricted differentiation potential. The function of these cell populations is to increase the number of differentiated cells produced after each stem cell division. Stem cells have the ability to self-renew. They are cells with unlimited or immortal self-renewal capabilities, capable of producing at least one type of highly differentiated daughter cells. Stem cells can produce daughter cells with the same phenotype and genotype as themselves, as well as specialized cells that make up body tissues and organs. They can also differentiate into progenitor cells. With the advancement of current technology, stem cells are used to culture human tissues for organ repair. Before stem cells can repair nerve cells, their activity needs to be tested using a detection device.

[0003] When using the detection device for targeted induction of neural cell repair stem cells, the device cannot accurately position the stem cell detection probe, leading to potential deviations in stem cell detection. Furthermore, the inability to wipe the probe after use results in contamination. Additionally, the device cannot provide double fixation for the stem cell culture dish, causing it to wobble and reducing the stability of stem cell detection. Summary of the Invention

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a detection device for stem cell targeted induction in nerve cell repair. The stem cell detection probe moves downwards continuously, allowing it to fit into the gap between the wiping cloths. The continuous movement of the probe causes the positioning plates to be compressed, causing them to swing downwards and press against a first spring. This separation of the positioning plates positions the stem cell detection probe. After stem cell detection is complete, the probe moves upwards, reducing the pressure on the positioning plates and allowing the wiping cloth to adhere to the surface of the stem cell detection probe for thorough wiping, ensuring the probe remains dry and clean. The advantages of this stem cell detection probe include dryness, a stable squeezing plate that can be pushed by a pusher plate to pull the second spring, ensuring the stable squeezing plate fully adheres to the surface of the stem cell culture dish, and the ability to push the connecting metal plate to allow the pre-fixing block to penetrate the interior of the culture dish positioning seat and fit into the interior of the fitting opening to pre-fix the support plate. Tightening the bolts allows the bolts to penetrate the interior of the culture dish positioning seat and extend into the interior of the threaded seat to fix the support plate. This results in greater stability of stem cells during detection, solving problems such as probe deviation, probe contamination, and shaking of the stem cell culture dish.

[0006] (II) Technical Solution

[0007] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a stem cell detection box, wherein a slider is slidably connected inside the stem cell detection box, a culture dish limiting seat is fixedly connected to the side surface of the slider, a stem cell culture dish is fitted inside the culture dish limiting seat, a fixing plate is fixedly connected to the upper surface of the culture dish limiting seat, a positioning plate is movably connected to the rear surface of the fixing plate via a bearing, a positioning rubber seat is provided on the side surface of the positioning plate, a wiping cleaning cloth is provided on the side surface of the positioning rubber seat, and a first spring is fixedly connected to the lower surface of the positioning plate, and the first spring is fixedly connected to the culture dish limiting seat;

[0008] A limiting threaded rod is fixedly connected to the upper surface of the stem cell detection box. A connecting plate is slidably connected to the end of the limiting threaded rod away from the stem cell detection box. A top plate is fixedly connected to the side surface of the connecting plate. A mounting base is fixedly connected to the lower surface of the top plate. A stem cell detection fixing rod is fixedly connected to the lower surface of the mounting base. A stem cell detection probe is provided at the end of the stem cell detection fixing rod away from the mounting base.

[0009] Preferably, a sliding plate is slidably connected inside the culture dish limiting and placement seat, and a second spring is fixedly connected to the side surface of the sliding plate, and the second spring is fixedly connected to the inside of the culture dish limiting and placement seat.

[0010] Preferably, a push plate is fixedly connected to the lower surface of the slide plate, and the push plate is slidably connected to the culture dish limiting placement seat. A stabilizing squeezing plate is fixedly connected to the side surface of the push plate, and the stabilizing squeezing plate is in contact with the stem cell culture dish.

[0011] Preferably, a support plate is fixedly connected to the lower surface of the push plate, and the support plate is slidably connected to the culture dish limiting and placement seat. Threaded seats are fixedly connected to both sides of the support plate, and the front surface of the support plate is provided with an interlocking opening.

[0012] Preferably, the front surface of the culture dish limiting placement seat is threaded with a bolt, and the bolt is threadedly connected to the threaded seat. A connecting metal plate is sleeved on the side surface of the bolt, and a pre-fixing block is fixedly connected to the rear surface of the connecting metal plate, and the pre-fixing block is fitted into the fitting port.

[0013] Preferably, the front surface of the stem cell detection box is movably connected to a sealed box door via a hinge, and a control panel is provided on the front surface of the sealed box door, and the control panel is electrically connected to the stem cell detection probe.

[0014] Preferably, an L-shaped plate is fixedly connected to the front surface of the sealed box door, and a fastening bolt is threaded into the interior of the L-shaped plate.

[0015] Preferably, a connecting seat is fixedly connected to the side surface of the stem cell detection box, and the connecting seat is threadedly connected to the fastening bolt.

[0016] Preferably, the upper surface of the stem cell detection box is provided with an opening, and the opening is located below the mounting base.

[0017] Preferably, the side surface of the limiting threaded rod is threaded with a limiting nut, and the limiting nut is in contact with the connecting plate.

[0018] Compared with the prior art, the present invention provides a detection device for stem cell targeted induction for neural cell repair, which has the following beneficial effects:

[0019] 1. In this invention, when detecting stem cells in a stem cell culture dish, the stem cell detection probe is continuously moved downwards to fit into the gap between the wiping cloth and the probe. The continuous movement of the probe causes the positioning plate to be squeezed downwards, which in turn squeezes the first spring, allowing the positioning plates to separate and position the stem cell detection probe. After the stem cell detection is completed, the probe can be moved upwards, reducing the squeezing force on the positioning plate and allowing the wiping cloth to fit into the surface of the stem cell detection probe for wiping, ensuring the dryness of the probe.

[0020] 2. This invention utilizes the connection between the sliding plate and the second spring, so that when the stabilizing compression plate is squeezed and pushed, the stabilizing compression plate can use the pushing plate to push the sliding plate, so that the sliding plate can pull the second spring, so that the stabilizing compression plate can completely adhere to and squeeze the surface of the stem cell culture dish. Pushing the connecting metal plate allows the pre-fixing block to penetrate the interior of the culture dish limiting placement seat and fit into the interior of the fitting opening to pre-fix the support plate. Tightening the bolt can penetrate the interior of the culture dish limiting placement seat and extend into the interior of the threaded seat to fix the support plate, making the stem cells more stable during detection. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the top plate structure of the present invention;

[0023] Figure 3 This is a schematic diagram of the internal structure of the stem cell detection box of the present invention;

[0024] Figure 4 This is a schematic diagram of the internal structure of the petri dish limiting and placement seat of the present invention;

[0025] Figure 5 for Figure 4 Schematic diagram of the structure at point A in the middle;

[0026] Figure 6 This is a schematic diagram of the rear view of the connecting metal plate structure of the present invention.

[0027] The components include: 1. Stem cell detection chamber; 2. Limiting threaded rod; 3. Connecting plate; 4. Top plate; 5. Limiting nut; 6. Control plate; 7. Sealing door; 8. L-shaped plate; 9. Fastening bolt; 10. Connecting seat; 11. Mounting seat; 12. Stem cell detection fixing rod; 13. Stem cell detection probe; 14. Opening; 15. Stem cell culture dish; 16. Connecting metal plate; 17. Sliding block; 18. Culture dish limiting placement seat; 19. Stabilizing compression plate; 20. Fixing plate; 21. First spring; 22. Positioning plate; 23. Wiping cleaning cloth; 24. Positioning rubber seat; 25. Bolt; 26. Pre-fixing block; 27. Slide plate; 28. Push plate; 29. ​​Second spring; 30. Support plate; 31. Threaded seat; 32. Fitting opening. Detailed Implementation

[0028] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Please see Figure 1-6 The system includes a stem cell testing chamber 1. A slider 17 is slidably connected inside the stem cell testing chamber 1. A culture dish positioning seat 18 is fixedly connected to the side surface of the slider 17. A stem cell culture dish 15 is fitted inside the culture dish positioning seat 18. A fixing plate 20 is fixedly connected to the upper surface of the culture dish positioning seat 18. A positioning plate 22 is movably connected to the rear surface of the fixing plate 20 via a bearing. A positioning rubber seat 24 is provided on the side surface of the positioning plate 22. A wiping cloth 23 is provided on the side surface of the positioning rubber seat 24. A first spring 21 is fixedly connected to the lower surface of the positioning plate 22, and the first spring 21 is fixedly connected to the culture dish positioning seat 18. By sliding the slider 17 against the stem cell testing chamber 1, the culture dish positioning seat 18 can be pulled out from inside the stem cell testing chamber 1. The surface of the culture dish limiting and placing seat 18 is recessed, allowing the stem cell culture dish 15 to be placed in the recess for detection. When the device detects stem cells in the stem cell culture dish 15, the stem cell detection probe 13 moves downward continuously, allowing it to fit into the gap between the wiping and cleaning cloth 23. The continuous movement of the monitoring probe causes the positioning plate 22 to be squeezed and swing downward, squeezing the first spring 21, thus separating the positioning plates 22 and positioning the stem cell detection probe 13. After the stem cell detection is completed, the stem cell detection probe 13 can move upward, reducing the squeezing force on the positioning plate 22, allowing the wiping and cleaning cloth 23 to fit into the surface of the stem cell detection probe 13 for wiping, ensuring the dryness of the stem cell detection probe 13.

[0030] A sliding plate 27 is slidably connected inside the culture dish limiting and placement seat 18. A second spring 29 is fixedly connected to the side surface of the sliding plate 27 and is fixedly connected to the inside of the culture dish limiting and placement seat 18. A push plate 28 is fixedly connected to the lower surface of the sliding plate 27 and is slidably connected to the culture dish limiting and placement seat 18. A stabilizing compression plate 19 is fixedly connected to the side surface of the push plate 28 and is in contact with the stem cell culture dish 15. A support plate 30 is fixedly connected to the lower surface of the push plate 28 and is slidably connected to the culture dish limiting and placement seat 18. Threaded seats 31 are fixedly connected to both sides of the support plate 30. The front surface of the support plate 30 has a fitting opening 32. A bolt 25 is threadedly connected to the front surface of the culture dish limiting and placement seat 18 and is threadedly connected to the threaded seat 31. A connecting metal plate is sleeved on the side surface of the bolt 25. 16. A pre-fixing block 26 is fixedly connected to the rear surface of the connecting metal plate 16, and the pre-fixing block 26 is fitted into the fitting port 32. When the stem cell culture dish 15 is placed inside the culture dish limiting placement seat 18, the connection between the sliding plate 27 and the second spring 29 is used to push the stabilizing compression plate 19. The stabilizing compression plate 19 can push the sliding plate 27 with the pushing plate, so that the sliding plate 27 can pull the second spring 29, so that the stabilizing compression plate 19 can be completely pressed against the surface of the stem cell culture dish 15. The connecting metal plate 16 is pushed so that the pre-fixing block 26 can penetrate the interior of the culture dish limiting placement seat 18 and fit into the interior of the fitting port 32 to pre-fix the support plate 30. Tightening the bolt 25 can penetrate the interior of the culture dish limiting placement seat 18 and extend into the interior of the threaded seat 31 to fix the support plate 30, so that the stem cells are more stable during detection.

[0031] A limiting threaded rod 2 is fixedly connected to the upper surface of the stem cell detection chamber 1. A limiting nut 5 is threadedly connected to the side surface of the limiting threaded rod 2, and the limiting nut 5 is in contact with the connecting plate 3. The end of the limiting threaded rod 2 away from the stem cell detection chamber 1 is slidably connected to the connecting plate 3. A top plate 4 is fixedly connected to the side surface of the connecting plate 3. A mounting base 11 is fixedly connected to the lower surface of the top plate 4. A stem cell detection fixing rod 12 is fixedly connected to the lower surface of the mounting base 11. A stem cell detection probe 13 is provided at the end of the stem cell detection fixing rod 12 away from the mounting base 11. An opening 14 is provided on the upper surface of the stem cell detection chamber 1, and the opening 14 is located below the mounting base 11. A sealing door 7 is movably connected to the front surface of the stem cell detection chamber 1 via a hinge. A control plate 6 is provided on the front surface of the sealing door 7, and the control plate 6 is electrically connected to the stem cell detection probe 13. After the stem cell culture dish 15 is fixed, the limiting nut 5 is turned to separate it from the surface of the connecting plate 3. Pushing the top plate 4 downwards allows the stem cell detection fixing rod 12 to drive the stem cell detection probe 13 through the opening 14 into the interior of the stem cell detection chamber 1, enabling the stem cell detection probe 13 to enter the interior of the stem cell culture dish 15 for stem cell detection. The stem cell detection probe 13 transmits the stem cell detection results to the control board 6, which allows the staff to view and record the results. An L-shaped plate 8 is fixedly connected to the front surface of the sealed chamber door 7, and a fastening bolt 9 is threaded inside the L-shaped plate 8. A connecting seat 10 is fixedly connected to the side surface of the stem cell detection chamber 1, and the connecting seat 10 is threadedly connected to the fastening bolt 9. After the stem cell culture dish 15 is placed, the movement of the sealed chamber door 7 allows it to engage with the stem cell detection chamber 1, so that the L-shaped plate 8 aligns with the connecting seat 10. Tightening the fastening bolt 9 allows the L-shaped plate 8 to pass through and threadedly connect to the connecting seat 10, thereby fixing the sealed chamber door 7.

[0032] In use, the stem cell culture dish 15 is placed in the recess of the culture dish limiting seat 18. Utilizing the connection between the sliding plate 27 and the second spring 29, the stabilizing compression plate 19 is pushed by the pushing plate, which in turn pushes the sliding plate 27, causing the sliding plate 27 to pull the second spring 29. This allows the stabilizing compression plate 19 to fully adhere to the surface of the stem cell culture dish 15. Pushing the connecting metal plate 16 allows the pre-fixing block 26 to penetrate the interior of the culture dish limiting seat 18 and fit into the fitting opening 32, pre-fixing the support plate 30. Tightening the bolt 25 allows it to penetrate the interior of the culture dish limiting seat 18 and extend into the threaded seat 31 to fix the support plate 30. After the stem cell culture dish 15 is fixed, tightening the limiting nut 5 separates it from the surface of the connecting plate 3. Pushing the top plate 4 downwards allows the stem cell detection fixing rod 12 to drive the stem cell detection probe 13 through the opening 14. The stem cell detection probe 13 is inserted into the stem cell detection chamber 1. It moves downwards continuously, allowing it to fit against the gap between the wiping cloth 23 and the probe. The continuous movement of the probe causes the positioning plate 22 to be compressed, causing it to swing downwards and press against the first spring 21. This allows the positioning plates 22 to separate and position the stem cell detection probe 13. The probe 13 can then enter the stem cell culture dish 15 to detect stem cells. The detection results are transmitted to the control board 6, which allows the results to be viewed and recorded by staff. After the stem cell detection is complete, the probe 13 moves upwards, reducing the pressure on the positioning plate 22 and allowing the wiping cloth 23 to adhere to the surface of the probe 13 for wiping, ensuring the probe 13 remains dry.

[0033] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A detection device for stem cell targeted induction for neural cell repair, comprising a stem cell detection chamber (1), characterized in that: The stem cell detection box (1) is internally connected to a slider (17), and a culture dish limiting seat (18) is fixedly connected to the side surface of the slider (17). A stem cell culture dish (15) is embedded in the culture dish limiting seat (18). A fixing plate (20) is fixedly connected to the upper surface of the culture dish limiting seat (18). A positioning plate (22) is movably connected to the rear surface of the fixing plate (20) through a bearing. A positioning rubber seat (24) is provided on the side surface of the positioning plate (22). A wiping cleaning cloth (23) is provided on the side surface of the positioning rubber seat (24). A first spring (21) is fixedly connected to the lower surface of the positioning plate (22), and the first spring (21) is fixedly connected to the culture dish limiting seat (18). A limiting threaded rod (2) is fixedly connected to the upper surface of the stem cell detection box (1). A connecting plate (3) is slidably connected to the end of the limiting threaded rod (2) away from the stem cell detection box (1). A top plate (4) is fixedly connected to the side surface of the connecting plate (3). A mounting base (11) is fixedly connected to the lower surface of the top plate (4). A stem cell detection fixing rod (12) is fixedly connected to the lower surface of the mounting base (11). A stem cell detection probe (13) is provided at the end of the stem cell detection fixing rod (12) away from the mounting base (11).

2. The detection device for stem cell targeted induction for neural cell repair according to claim 1, characterized in that: The interior of the culture dish limiting and placement seat (18) is slidably connected to a slide plate (27), and a second spring (29) is fixedly connected to the side surface of the slide plate (27), and the second spring (29) is fixedly connected to the interior of the culture dish limiting and placement seat (18).

3. The detection device for stem cell targeted induction for neural cell repair according to claim 2, characterized in that: The lower surface of the slide plate (27) is fixedly connected to a push plate (28), and the push plate (28) is slidably connected to the culture dish limiting placement seat (18). The side surface of the push plate (28) is fixedly connected to a stabilizing squeezing plate (19), and the stabilizing squeezing plate (19) is attached to the stem cell culture dish (15).

4. The detection device for stem cell targeted induction for neural cell repair according to claim 3, characterized in that: The lower surface of the push plate (28) is fixedly connected to a support plate (30), and the support plate (30) is slidably connected to the culture dish limiting placement seat (18). Both sides of the support plate (30) are fixedly connected to threaded seats (31), and the front surface of the support plate (30) is provided with a fitting opening (32).

5. The detection device for stem cell targeted induction for neural cell repair according to claim 1, characterized in that: The front surface of the culture dish limiting placement seat (18) is threaded with a bolt (25), and the bolt (25) is threaded with a threaded seat (31). A connecting metal plate (16) is sleeved on the side surface of the bolt (25), and a pre-fixing block (26) is fixedly connected to the rear surface of the connecting metal plate (16), and the pre-fixing block (26) is fitted into the fitting port (32).

6. The detection device for stem cell targeted induction for neural cell repair according to claim 1, characterized in that: The front surface of the stem cell detection box (1) is connected to a sealed box door (7) via a hinge. A control board (6) is provided on the front surface of the sealed box door (7), and the control board (6) is electrically connected to the stem cell detection probe (13).

7. The detection device for stem cell targeted induction for neural cell repair according to claim 6, characterized in that: An L-shaped plate (8) is fixedly connected to the front surface of the sealed box door (7), and a fastening bolt (9) is threaded inside the L-shaped plate (8).

8. The detection device for stem cell targeted induction for neural cell repair according to claim 1, characterized in that: The side surface of the stem cell detection box (1) is fixedly connected to a connecting seat (10), and the connecting seat (10) is threadedly connected to a fastening bolt (9).

9. The detection device for stem cell targeted induction for neural cell repair according to claim 1, characterized in that: The upper surface of the stem cell detection box (1) is provided with an opening (14), and the opening (14) is located below the mounting base (11).

10. The detection device for stem cell targeted induction for neural cell repair according to claim 1, characterized in that: The side surface of the limiting threaded rod (2) is threadedly connected to a limiting nut (5), and the limiting nut (5) is in contact with the connecting plate (3).

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