In-vitro organ preservation and culture device

Abstract

The invention discloses an in-vitro organ preservation and culture device, and belongs to the technical field of organ culture. By pressing an air bag seat, air can be guided into three air bag rings to expand the three air bag rings, and the inner walls of the expanded air bag rings are in contact with the outside of an organ to play a role in transversely limiting the organ; the air in the air bag ring can be inflated into the rubber sleeves to enable the rubber sleeves to expand, the multiple rubber sleeves can be fully attached to the outer surface of the organ, the hollow barrier strip can also expand and be attached to the outer portion of the organ, the effect of vertically limiting the organ can be achieved, and the organ can be wrapped transversely and vertically in multiple directions, so that the organ can be prevented from being damaged. The organ can be flexibly supported by the storage limiting assembly, so that the storage limiting assembly can be adaptively adjusted according to the external shape of the organ, the organ can be limited and stored under the condition of not causing pressure damage to the organ, and the situation that the organ is shaken by external force or is excessively extruded to cause damage to influence the storage and culture effect is prevented.

Description

Technical Field

[0001] This invention belongs to the field of organ culture technology, specifically an in vitro organ preservation and culture device. Background Technology

[0002] Currently, using stem cell technology to construct living tissues and organs is one of the ideal ways to solve the shortage of organ donors and to construct disease pathology models. Constructing tissues and organs through bio-3D printing technology, and then preserving and culturing them in vitro and monitoring various physicochemical indicators, is an important task in organ manufacturing research.

[0003] In the preservation and culture of organs in vitro, organs are usually placed in containers and preserved using a perfusion method. By simulating physiological circulation, nutrients and oxygen are provided to the organs while metabolic waste is removed. This significantly improves the preservation of organ activity and condition. By constructing tissue organs to simulate the forces experienced by organs in the human body, the container exerts forces on the internal organs during transport or collisions. Furthermore, the organs are prone to shaking and impacting the inner wall of the container, or the tissues within the organs may be excessively compressed and rubbed against each other, leading to organ damage and affecting the preservation and culture results. Summary of the Invention

[0004] To overcome the above-mentioned defects, the present invention provides an in vitro organ preservation and culture device, which solves the problem that when constructing tissue organs to simulate the forces on organs in the human body, the container will exert forces on the internal organs during transportation or collision, and the organs are prone to shaking and impacting the inner wall of the container, or the tissues in the organs will be excessively squeezed and rubbed against each other, resulting in organ damage and thus affecting the effectiveness of organ preservation and culture.

[0005] The purpose of this invention is to wrap the organ in multiple directions, both horizontally and vertically, so that the organ can be flexibly supported by the preservation and limiting components. Therefore, the preservation and limiting components can adaptively adjust according to the external shape of the organ, so as to limit and preserve the organ without causing pressure damage, and prevent the organ from shaking or being excessively squeezed by external forces, which would affect the preservation and culture effect.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an in vitro organ preservation and culture device, comprising an incubator, a base fixedly connected to the bottom of the incubator, and a cover plate fixedly installed on the top of the incubator, a support assembly provided below the cover plate, the support assembly being installed inside the incubator, three insert rods fixedly connected to the bottom of the inner wall of the incubator, the insert rods being inserted through the bottom of the support assembly, three preservation limiting components installed inside the support assembly, and a pressurization component being connected to the top of the three preservation limiting components through the cover plate;

[0007] The pressurization assembly is fixedly installed on the top of the cover plate. The outer wall of the cover plate is threaded with fastening screws around its perimeter. The fastening screws penetrate one side of the cover plate and are connected to the outer side of the top of the incubator. Three culture perfusion lines are installed equidistantly on the top of the cover plate. The bottom end of the culture perfusion lines extends to the space between the inner wall of the incubator and the support assembly.

[0008] As a further embodiment of the present invention: the mounting assembly includes a tray, a fixed frame is fixedly connected to the tray, a handle is fixedly connected to the top of the fixed frame, and three storage slots are equidistantly provided on the outside of the fixed frame, the included angle between the three storage slots is 120 degrees, the storage slots are arc-shaped and the inner wall is fixed to the storage limiting assembly.

[0009] As a further embodiment of the present invention: the inner wall of the storage slot is provided with a plurality of through holes, the tray is provided with a plurality of overflow holes corresponding to the lower part of the storage slot, and the bottom of the tray is provided with a locking hole corresponding to the position of a plurality of insert rods, and the insert rods are inserted into the locking hole.

[0010] As a further aspect of the present invention: the preservation limiting component includes three rings, an airbag ring is fixed inside the rings, the inner wall of the airbag ring is provided with several rubber sleeves, the outer side of the rubber sleeves communicates with the inside of the airbag ring, and two air guide tubes are snapped between two adjacent rings.

[0011] As a further embodiment of the present invention: the two ends of the air guide tube pass through the ring and are connected to two adjacent airbag rings, and two hollow baffles are connected between the two adjacent airbag rings. The top of the uppermost airbag ring is connected to a connecting pipe, and the top end of the connecting pipe passes through the cover plate and is connected to the end of the pressurization assembly.

[0012] As a further aspect of the present invention: the pressurization assembly includes an airbag seat, the airbag seat is fixed to the top of the cover plate, a one-way valve is installed on the outer side of the top of the airbag seat, the one-way valve is used to introduce external gas into the airbag seat and prevent the gas inside the airbag seat from escaping, and three connecting pipes are connected to the outer side of the bottom of the airbag seat, the end of the connecting pipe away from the airbag seat is connected to the top end of the connecting pipe in the storage limiting assembly.

[0013] As a further embodiment of the present invention: an air valve and a second one-way valve are installed on the outer wall of the connecting pipe near the airbag seat. The air valve is located between the second one-way valve and the airbag seat. The second one-way valve is used to allow the gas inside the airbag seat to pass through the connecting pipe into the connecting pipe, and the second one-way valve prevents the gas inside the connecting pipe from flowing back into the airbag seat.

[0014] As a further aspect of the present invention: a cavity is provided in the side wall of the incubator, an observation window is provided on the front of the incubator, and three industrial cameras are installed on the top of the inner wall of the cover plate and above the corresponding mounting components.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0016] 1. In this invention, by connecting the connecting tube in the pressurization assembly to the connecting tube in the preservation limiting assembly, when limiting the organ, pressing the airbag seat allows the gas inside the airbag seat to be introduced into the connecting tube through the connecting tube and the one-way valve, so that the gas can be introduced into the three airbag rings and inflated. The inner wall of the inflated airbag ring will contact the outside of the organ, playing a role in lateral limiting of the organ. The outer side of the rubber sleeve is connected to the inside of the airbag ring, so that the gas inside the airbag ring can fill the rubber sleeve and cause it to inflate. Multiple rubber sleeves can fully fit the outer surface of the organ. Two hollow baffles are located between the two airbag rings and are interconnected. The hollow baffles can also inflate and fit the outside of the organ, playing a role in vertical limiting of the organ. By wrapping the organ in multiple directions, both laterally and vertically, the organ can be flexibly supported by the preservation limiting assembly. Therefore, the preservation limiting assembly can adaptively adjust according to the external shape of the organ, so as to limit and preserve the organ without causing pressure damage, preventing the organ from shaking or being excessively squeezed by external forces, which would affect the preservation and culture effect.

[0017] 2. In this invention, when the organ is released from its limiting state, the connecting tube is pulled out from the top of the connecting tube, allowing the gas inside the airbag to be discharged through the connecting tube. The airbag returns to its original state, thus facilitating the release of the organ's limiting state. After opening the cover, the fixing frame and tray are removed from the incubator using the handle on the mounting assembly, making it easy to remove the organ. When placing the organ, it is placed in the storage slot inside the fixing frame, so that the organ is located inside the storage limiting assembly, and the bottom of the organ is in contact with the tray. Next, the culture perfusion lines are connected to the veins, arteries, or secretion ports of the organ through the through holes outside the fixing frame. Multiple culture perfusion lines are separated through multiple through holes to prevent tangling. The fixing frame is placed into the incubator with the organ connected to it. The fixing frame then drives the tray to slide down inside the incubator, so that the locking holes on the tray correspond to the insertion rods below. The insertion of the three insertion rods into the locking holes achieves the purpose of limiting the tray and fixing frame, making it difficult for the fixing frame to cause the organ to rotate freely in the incubator, thus improving the stability of the placed organ. Attached Figure Description

[0018] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0019] Figure 2 This is a schematic diagram of the cross-section of the present invention;

[0020] Figure 3 For the present invention Figure 2 Enlarged structural diagram at point A;

[0021] Figure 4 This is a schematic diagram of the structure of the mounting components of the present invention viewed from below;

[0022] Figure 5 This is a schematic diagram of the structure of the limiting component of the present invention;

[0023] Figure 6 This is a three-dimensional structural diagram of the mounting components of the present invention;

[0024] Figure 7 This is a schematic diagram of the cover plate of the present invention viewed from below;

[0025] In the diagram: 1. Incubator; 2. Base; 3. Cover plate; 4. Support assembly; 401. Tray; 402. Fixing frame; 403. Handle; 404. Storage slot; 405. Through hole; 406. Overflow hole; 407. Locking hole; 5. Insert rod; 6. Storage limiting assembly; 601. Ring; 602. Air duct; 603. Hollow baffle; 604. Airbag ring; 605. Rubber sleeve; 606. Connecting pipe; 7. Pressurization assembly; 701. Airbag seat; 702. One-way valve one; 703. Connecting pipe; 704. Air valve; 705. One-way valve two; 8. Cavity; 9. Industrial camera; 10. Fastening screw; 11. Culture perfusion line; 12. Observation window. Detailed Implementation

[0026] The technical solution of this application will be further described in detail below with reference to specific embodiments.

[0027] like Figure 1-7 As shown, the present invention provides a technical solution: an in vitro organ preservation and culture device, including an incubator 1, a base 2 fixedly connected to the bottom of the incubator 1, and a cover plate 3 fixedly installed on the top of the incubator 1. A support assembly 4 is provided below the cover plate 3 and is installed inside the incubator 1. The support assembly 4 includes a tray 401, a fixing frame 402 fixedly connected to the tray 401, a handle 403 fixedly connected to the top of the fixing frame 402, and three placement slots 404 equidistantly provided outside the fixing frame 402. The included angle between the three placement slots 404 is 120 degrees. The placement slots 404 are arc-shaped and their inner walls are fixed to the preservation limiting assembly 6. When an organ is placed in the placement slot 404 inside the fixing frame 402, the organ is located inside the preservation limiting assembly 6, and the bottom of the organ is in contact with the tray 401. The organs are separated by multiple placement slots 404, so that each organ can be preserved independently and prevent friction damage between organs.

[0028] The inner wall of the placement tank 404 has several through holes 405, through which the solution is guided to ensure that the solution can fully contact all parts of the organ. The tray 401 has several overflow holes 406 corresponding to the lower part of the placement tank 404, and the bottom of the tray 401 has locking holes 407 corresponding to the positions of several insertion rods 5. The insertion rods 5 are inserted into the locking holes 407. The fixing frame 402 drives the tray 401 to slide down inside the incubator 1, so that the locking holes 407 on the tray 401 correspond to the insertion rods 5 below. By inserting three insertion rods 5 into the locking holes 407, the tray 401 and the fixing frame 402 are limited, so that the fixing frame 402 does not easily cause the organ to rotate freely in the incubator 1, thus improving the stability of the placed organ.

[0029] Three rods 5 are fixedly connected to the bottom of the inner wall of the incubator 1. The rods 5 are inserted through the bottom of the support assembly 4. Three preservation and limiting components 6 are installed inside the support assembly 4. The preservation and limiting components 6 include three rings 601. An air bladder ring 604 is fixed inside the ring 601. The inner wall of the air bladder ring 604 is provided with several rubber sleeves 605. The outer side of the rubber sleeve 605 is connected to the inside of the air bladder ring 604. Two air guide tubes 602 are snapped between two adjacent rings 601. The inner wall of the inflated air bladder ring 604 will contact the outside of the organ, which will play a role in lateral limiting of the organ. The outer side of the rubber sleeve 605 is connected to the inside of the air bladder ring 604, so that the gas inside the air bladder ring 604 can fill the rubber sleeve 605 and cause it to expand. Multiple rubber sleeves 605 can fully fit the outer surface of the organ.

[0030] The two ends of the air tube 602 pass through the ring 601 and are connected to the two adjacent airbag rings 604. Two hollow baffles 603 are connected between the two adjacent airbag rings 604. The top of the uppermost airbag ring 604 is connected to the connecting tube 606. The top of the connecting tube 606 passes through the cover plate 3 and is connected to the end of the pressurization component 7. The hollow baffles 603 are located between the two airbag rings 604 and are connected to each other. The hollow baffles 603 can also expand and fit against the outside of the organ, which can play a role in vertically limiting the organ.

[0031] The top of the three storage limiting components 6 penetrates the cover plate 3 and is connected to the pressurization component 7. The pressurization component 7 includes an airbag seat 701, which is fixed to the top of the cover plate 3. A one-way valve 702 is installed on the outer side of the top of the airbag seat 701. The one-way valve 702 is used to introduce external gas into the airbag seat 701 and prevent the gas inside the airbag seat 701 from escaping. Three connecting pipes 703 are connected to the outer side of the bottom of the airbag seat 701. The end of the connecting pipe 703 away from the airbag seat 701 is connected to the top of the connecting pipe 606 in the storage limiting component 6.

[0032] An air valve 704 and a one-way valve 705 are installed on the outer wall of the connecting pipe 703 near the airbag seat 701. Because the air valve 704 is provided, after the air valve 704 is closed, the gas inside the airbag seat 701 will not enter the connecting pipe 703, and the connecting pipe 703 will not guide air into the airbag ring 604 through the connecting pipe 606, thereby facilitating the adjustment of the expansion size of one or more airbag rings 604 at different positions.

[0033] The air valve 704 is located between the second check valve 705 and the airbag seat 701. The second check valve 705 is used to allow the gas inside the airbag seat 701 to enter the connecting pipe 606 through the connecting pipe 703, and the second check valve 705 prevents the gas inside the connecting pipe 703 from flowing back into the airbag seat 701. By pressing the airbag seat 701, the gas inside the airbag seat 701 can be introduced into the connecting pipe 606 through the connecting pipe 703 and the second check valve 705, and the second check valve 705 will prevent the gas inside the connecting pipe 703 from flowing back into the airbag seat 701. The connecting pipe 606 introduces the gas into the airbag ring 604 inside the ring 601. Releasing the airbag seat 701 restores it to its original state. The first check valve 702 is used to introduce external gas into the airbag seat 701 and prevent the gas inside the airbag seat 701 from escaping, so that the gas can be drawn into the airbag seat 701.

[0034] The pressurization assembly 7 is fixedly installed on the top of the cover plate 3. The outer wall of the cover plate 3 is threaded with fastening screws 10. The fastening screws 10 pass through one side of the cover plate 3 and are connected to the outer side of the top of the incubator 1. Three culture perfusion lines 11 are installed equidistantly on the top of the cover plate 3. The multiple culture perfusion lines 11 are separated by multiple through holes 405 to prevent the culture perfusion lines 11 from getting tangled. The bottom end of the culture perfusion lines 11 extends to the inner wall of the incubator 1 and between the support assembly 4. A cavity 8 is opened in the side wall of the incubator 1 to provide shock resistance and improve the heat preservation effect. An observation window 12 is provided on the front of the incubator 1. Three industrial cameras 9 are installed on the top of the inner wall of the cover plate 3 and above the support assembly 4. The culture perfusion lines 11 are controlled by the dynamic perfusion system to perfuse the organs. The industrial cameras 9 on the inner wall of the cover plate 3 record the entire culture process and can be used to observe the internal culture situation through the observation window 12 on the outside of the incubator 1.

[0035] The working principle of this invention is as follows:

[0036] When preserving and culturing organs in vitro, the locking state between the cover plate 3 and the incubator 1 is released by loosening the fastening screws 10 on the outside of the cover plate 3. After opening the cover plate 3, the fixing frame 402 and the tray 401 are taken out of the incubator 1 by the handle 403 on the mounting component 4. The tissue organ constructed by bio-3D printing technology is placed in the placement slot 404 inside the fixing frame 402, so that the organ is located inside the preservation limiting component 6 and the bottom of the organ is in contact with the tray 401. Then, the culture perfusion line 11 is connected to the vein, artery or secretion port of the organ through the through hole 405 outside the fixing frame 402. Multiple culture perfusion lines 11 are separated by multiple through holes 405 to prevent the culture perfusion lines 11 from getting tangled. The other end of the culture perfusion line 11 is connected to the external dynamic perfusion system.

[0037] After the organ placement is completed, the fixation frame 402 is placed into the incubator 1 via the handle 403. The fixation frame 402 then drives the tray 401 to slide down inside the incubator 1, so that the locking holes 407 on the tray 401 correspond to the insertion rods 5 below. By inserting the three insertion rods 5 into the locking holes 407, the tray 401 and the fixation frame 402 are limited, making it difficult for the fixation frame 402 to cause the organ to rotate freely in the incubator 1, thus improving the stability of the placed organ.

[0038] Next, inject preservation solution or preservation gel into the incubator 1, ensuring that the preservation solution or preservation gel covers the top of the organ. The solution is then guided through the through-hole 405 in the placement slot 404 to ensure sufficient contact between the solution and all parts of the organ. Next, install the cover plate 3 on the top of the incubator 1 and secure it to the incubator 1 using multiple fastening screws 10. Connect the connecting pipe 703 in the pressurization assembly 7 to the connecting pipe 606 in the preservation limiting assembly 6. When limiting the organ, press the airbag. Airbag seat 701, the gas inside the airbag seat 701 can be introduced into the connecting pipe 606 through the connecting pipe 703 and the one-way valve 2 705, and the one-way valve 2 705 will prevent the gas inside the connecting pipe 703 from flowing back into the airbag seat 701. The connecting pipe 606 introduces the gas into the airbag ring 604 inside the ring 601. The airbag seat 701 is released to restore its original state. The one-way valve 1 702 is used to introduce external gas into the airbag seat 701 and prevent the gas inside the airbag seat 701 from escaping, so that the gas can be drawn into the airbag seat 701.

[0039] The two ends of the air tube 602 pass through the ring 601 and are connected to the two adjacent airbag rings 604, so that gas can be introduced into the three airbag rings 604 to inflate. The inner wall of the inflated airbag ring 604 will contact the outside of the organ, which will play a role in lateral limiting of the organ. The outer side of the rubber sleeve 605 is connected to the inside of the airbag ring 604, so that the gas inside the airbag ring 604 can fill the rubber sleeve 605 to inflate it. Multiple rubber sleeves 605 can fully fit the outer surface of the organ. Two hollow baffles 603 are located between the two airbag rings 604 and are connected to each other. The hollow baffles 603 can also inflate and fit the outside of the organ, which can play a role in vertical limiting of the organ. By wrapping the organ in multiple directions in the lateral and vertical directions, the organ can be flexibly supported by the preservation and limiting component 6.

[0040] During organ culture, the culture perfusion pipeline 11 is controlled by a dynamic perfusion system to perfuse the organ. The industrial camera 9 above the inner wall of the cover plate 3 records the entire culture process and can observe the internal culture conditions through the observation window 12 outside the incubator 1. When the organ is released from its limiting state, the connecting tube 703 is pulled out from the top of the connecting tube 606, allowing the gas inside the airbag ring 604 to be discharged through the connecting tube 606, and the airbag ring 604 returns to its original state to facilitate the removal of the tissue organ.

[0041] In the description of this invention, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0042] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0043] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a communication connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0044] In this invention, unless otherwise expressly specified and limited, the first feature "on" or "below" the second feature may be in direct contact with the first and second features, or indirect contact through an intermediate medium. In the description of this specification, references to terms such as "an embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

Claims

1. An in vitro organ preservation and culture device, comprising an incubator (1), characterized in that: The bottom of the incubator (1) is fixedly connected to a base (2), and the top of the incubator (1) is fixedly installed with a cover plate (3). A support assembly (4) is provided below the cover plate (3). The support assembly (4) is installed inside the incubator (1). Three insert rods (5) are fixedly connected to the bottom of the inner wall of the incubator (1). The insert rods (5) are inserted through the bottom of the support assembly (4). Three storage limiting components (6) are installed inside the support assembly (4). The top of the three storage limiting components (6) is connected to a pressurization assembly (7) through the cover plate (3). The pressurization assembly (7) is fixedly installed on the top of the cover plate (3). The outer wall of the cover plate (3) is threaded with fastening screws (10). The fastening screws (10) penetrate one side of the cover plate (3) and are connected to the outside of the top of the incubator (1). Three culture infusion lines (11) are installed equidistantly on the top of the cover plate (3). The bottom end of the culture infusion line (11) extends to the inner wall of the incubator (1) and the support assembly (4).

2. The in vitro organ preservation and culture device according to claim 1, characterized in that: The mounting component (4) includes a tray (401), a fixed frame (402) is fixedly connected to the tray (401), a handle (403) is fixedly connected to the top of the fixed frame (402), and three storage slots (404) are equidistantly provided on the outside of the fixed frame (402). The included angle between the three storage slots (404) is 120 degrees. The storage slots (404) are arc-shaped and their inner walls are fixed to the storage limiting component (6).

3. The in vitro organ preservation and culture device according to claim 2, characterized in that: The inner wall of the storage slot (404) is provided with several through holes (405), and the tray (401) is provided with several overflow holes (406) corresponding to the lower part of the storage slot (404). The bottom of the tray (401) is provided with locking holes (407) corresponding to the positions of several insert rods (5), and the insert rods (5) are inserted into the locking holes (407).

4. The in vitro organ preservation and culture device according to claim 1, characterized in that: The storage limiting component (6) includes three rings (601), an airbag ring (604) is fixed inside the ring (601), and the inner wall of the airbag ring (604) is provided with several rubber sleeves (605). The outer side of the rubber sleeves (605) communicates with the inside of the airbag ring (604), and two air guide tubes (602) are snapped between two adjacent rings (601).

5. The in vitro organ preservation and culture device according to claim 4, characterized in that: The two ends of the air guide tube (602) pass through the ring sleeve (601) and are connected to the two adjacent airbag rings (604). Two hollow baffles (603) are connected between the two adjacent airbag rings (604). The top of the uppermost airbag ring (604) is connected to the connecting tube (606). The top end of the connecting tube (606) passes through the cover plate (3) and is connected to the end of the pressurization assembly (7).

6. The in vitro organ preservation and culture device according to claim 5, characterized in that: The pressurization assembly (7) includes an airbag seat (701), which is fixed to the top of the cover plate (3). A one-way valve (702) is installed on the outer side of the top of the airbag seat (701). The one-way valve (702) is used to introduce external gas into the airbag seat (701) and prevent the gas inside the airbag seat (701) from escaping. Three connecting pipes (703) are connected to the outer side of the bottom of the airbag seat (701). The end of the connecting pipe (703) away from the airbag seat (701) is connected to the top end of the connecting pipe (606) in the storage limiting assembly (6).

7. The in vitro organ preservation and culture device according to claim 6, characterized in that: An air valve (704) and a one-way valve (705) are installed on the outer wall of the connecting pipe (703) near the airbag seat (701). The air valve (704) is located between the one-way valve (705) and the airbag seat (701). The one-way valve (705) is used to allow the gas inside the airbag seat (701) to pass through the connecting pipe (703) into the connecting pipe (606), and the one-way valve (705) prevents the gas inside the connecting pipe (703) from flowing back into the airbag seat (701).

8. The in vitro organ preservation and culture device according to claim 1, characterized in that: The incubator (1) has a cavity (8) inside its side wall, and an observation window (12) is provided on the front of the incubator (1). Three industrial cameras (9) are installed on the top of the inner wall of the cover plate (3) and above the corresponding mounting component (4).

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