Potassium-dissolving aspergillus tubingensis sk-1 and its application in woody plant cultivation

Abstract

The application discloses a potassium-dissolving Aspergillus tubingensis SK-1 and application thereof in woody plant cultivation, and belongs to the technical field of microorganisms. The Aspergillus tubingensis SK-1 is preserved in the China General Microbiological Culture Collection Center on August 12, 2025, is named as Aspergillus tubingensis, has a preservation number of CGMCC No. 42163, and is preserved in Beijing, China. The Aspergillus tubingensis SK-1 in the application can efficiently decompose insoluble potassium ore in soil, increase the content of soluble potassium elements in soil, promote the potassium absorption efficiency of woody plants, and thus promote the growth of the woody plants. When the Aspergillus tubingensis SK-1 is applied to the cultivation of woody plants, the potassium absorption efficiency of woody plants such as catalpa bungei and poplar can be significantly improved, and the woody plants have a significant growth-promoting effect on plant height.

Description

Technical Field

[0001] This invention relates to a potassium-solubilizing Aspergillus tabineus SK-1 and its application in the cultivation of woody plants, belonging to the field of microbial technology. Background Technology

[0002] Potassium is an essential macronutrient for plant growth. As a key activator of many enzymes, it participates in photosynthesis, respiration, and the synthesis and metabolism of carbohydrates, lipids, and proteins. It promotes root development and fruit ripening, increasing fruit yield and quality. Potassium enhances a plant's resistance to drought, cold, disease, and lodging by regulating ion balance, stomatal opening and closing, and thickening cell walls. Potassium deficiency in plants manifests as yellowing and scorching of older leaves, weak stems, smaller fruits, and decreased stress resistance. Woody plants, due to their large biomass and long growth cycle, have a significantly higher potassium requirement than herbaceous crops and are more dependent on potassium.

[0003] Although soils are rich in potassium minerals, most potassium exists in complex and insoluble silicate forms, which plants cannot directly utilize. Therefore, it is necessary to apply potassium fertilizers or potassium-solubilizing microorganisms appropriately. While chemical potassium fertilizers can provide short-term potassium replenishment, long-term application can easily lead to soil compaction. Potassium-solubilizing microorganisms in potassium-solubilizing agents can decompose insoluble potassium minerals in the soil by secreting organic acids and iron carriers, making it an effective and green means of potassium replenishment. Currently, most potassium-solubilizing microorganisms are primarily bacterial and developed for agricultural crops, not suitable for woody plants, while the development of fungal potassium-solubilizing microorganism resources is insufficient.

[0004] Chinese invention patent application CN118389295A discloses a strain of *Aspergillus tabineum* PTN02 and its applications. This strain can produce phytase at high yields and has a good effect on degrading phytic acid. Chinese invention patent CN114456944B discloses a *Aspergillus tabineum* strain and a compound microbial agent containing this strain, as well as its applications. This *Aspergillus tabineum* strain has a good ability to control cucumber wilt. Chinese invention patent application CN119120209A discloses a *Aspergillus tabineum* strain and its applications; this strain has the ability to solubilize phosphorus. Although *Aspergillus tabineum* has been used in phytic acid degradation, crop disease control, and phosphorus solubilization, its applications in potassium solubilization and woody plant cultivation remain unknown and require further research and exploration. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the purpose of this invention is to provide a potassium-releasing Aspergillus tabineus SK-1 and its application in the cultivation of woody plants. The Aspergillus tabineus SK-1 can relieve potassium and promote the potassium absorption efficiency of woody plants, thereby promoting the growth of woody plants.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution:

[0007] A potassium-solubilizing Aspergillus tubingensis strain SK-1 was deposited on August 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), classified as Aspergillus tubingensis, with accession number CGMCC No. 42163, and deposited in Beijing, China.

[0008] Furthermore, the potassium-solubilizing Aspergillus tabineis SK-1 contains the nucleotide sequence shown in SEQ ID NO.1.

[0009] Furthermore, the *Aspergillus tabingensis* SK-1 was isolated from the rhizosphere soil of perennial catalpa trees.

[0010] Application of potassium-solubilizing Aspergillus tabineus SK-1 in woody plant cultivation, the application includes the following steps:

[0011] (1) Potassium-solubilizing Aspergillus tabinei SK-1 was fermented to obtain Aspergillus tabinei SK-1 fermentation broth;

[0012] (2) Prepare the Aspergillus tabineus SK-1 fermentation broth obtained in step (1) into Aspergillus tabineus SK-1 inoculant and apply it to the planting soil of woody plants.

[0013] Further, in step (1), the preparation method of the fermentation broth of Aspergillus tabineus SK-1 is as follows: the Aspergillus tabineus SK-1 is inoculated into potato glucose culture medium and cultured under shaking conditions of 28℃ and 180rpm for 5-10 days to obtain the fermentation broth of Aspergillus tabineus SK-1.

[0014] Furthermore, in step (2), the Aspergillus tabineus SK-1 agent includes one of Aspergillus tabineus SK-1 liquid agent and Aspergillus tabineus SK-1 solid agent.

[0015] Furthermore, the preparation method of the Aspergillus tabineum SK-1 liquid inoculant is as follows: the Aspergillus tabineum SK-1 fermentation broth obtained in step (1) is diluted with water 10-100 times, and the viable count is >1×10⁻⁶. 8 CFU / mL.

[0016] Furthermore, the preparation method of the Aspergillus tabineus SK-1 solid inoculant is as follows: the Aspergillus tabineus SK-1 fermentation broth obtained in step (1) is mixed with corn straw powder and wheat bran powder in a ratio of 1mL:2g:6g, dried at 50℃ and then pulverized, with a viable count >1×10 8 CFU / g.

[0017] Furthermore, the potato glucose culture medium consists of: 200 g / L potato and 20 g / L glucose.

[0018] Furthermore, the application method of the Aspergillus tabineum SK-1 inoculant is as follows: during the transplanting period of tissue culture seedlings, apply Aspergillus tabineum SK-1 liquid inoculant to the roots of woody plants at a dosage of 10 mL / plant, or apply Aspergillus tabineum SK-1 solid inoculant mixed with soil at a dosage of 10 g / plant in holes; during the topdressing period of tissue culture seedling growth, apply Aspergillus tabineum SK-1 liquid inoculant to the roots of woody plants at a dosage of 200 mL / plant, or apply Aspergillus tabineum SK-1 solid inoculant mixed with soil at a dosage of 200 g / plant in holes.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] 1. This invention isolates potassium-solubilizing Aspergillus tabineus SK-1 from the rhizosphere soil of Catalpa trees, which can decompose insoluble potassium minerals in the soil and increase the content of soluble potassium in the soil.

[0021] 2. The Aspergillus tabineus SK-1 of the present invention, when applied to the cultivation of woody plants, can significantly improve the potassium absorption efficiency of woody plants such as catalpa and poplar, and has a significant effect on promoting the growth of woody plants and reducing the dependence on chemical fertilizers.

[0022] 3. The Aspergillus tabineus SK-1 of the present invention can promote the dissolution and release of insoluble potassium in the soil, improve the soil nutrient supply capacity, and can be developed as an excellent microbial agent to reduce the application of potassium fertilizer. It has broad application prospects in forestry production.

[0023] 4. The microbial agent prepared by Aspergillus tabineum SK-1 in this invention can effectively promote the growth of potassium-deficient plants and is suitable for nursery seedling cultivation, tree topdressing, forest soil improvement and ecological restoration projects, with significant ecological and economic benefits. Attached Figure Description

[0024] Figure 1 This is a diagram of the potassium-solubility zone of Aspergillus tabingensis strain SK-1;

[0025] Figure 2 This is a PDA plate colony morphology diagram of Aspergillus tabbini SK-1 strain;

[0026] Figure 3 This study investigates the effects of Aspergillus tabineum SK-1 liquid inoculant on the growth of potted Catalpa bungei seedlings. In Figure A, the front view of the potted Catalpa bungei seedlings is shown, and in Figure B, the top view of the potted Catalpa bungei seedlings is shown. In Figures A and B, the plants on the left are the control group of Catalpa bungei seedlings that have not been treated with Aspergillus tabineum SK-1 liquid inoculant, and the plants on the right are the treatment group of Catalpa bungei seedlings that have been treated with Aspergillus tabineum SK-1 liquid inoculant.

[0027] Figure 4This study investigates the effect of Aspergillus tabineum SK-1 solid inoculant on the growth of potted poplar seedlings under potassium deficiency stress. The plants on the left in the figure represent the poplar treatment group treated with Aspergillus tabineum SK-1 solid inoculant, while the plants on the right represent the poplar control group not treated with Aspergillus tabineum SK-1 solid inoculant. Detailed Implementation

[0028] Example 1: Isolation and Identification of Aspergillus tabineus SK-1 strain

[0029] (1) Isolation of strains

[0030] Rhizosphere soil collection: Perennial catalpa trees were selected from the Weizishan Catalpa Nature Reserve in Laishan District, Yantai City, Shandong Province. The roots of the soil layer below 10cm were dug up, the soil on the roots was shaken off and placed in a sterile self-sealing bag, and stored at 4℃ to obtain soil samples.

[0031] Isolation, purification, and culture: Take 5g of soil sample, add 45mL of sterile water, vortex, and then serially dilute to 1×10⁻⁶. -3 and 1×10 -4 100 μL of the soil dilution was spread onto potato dextrose agar (PDA) plates and incubated at 28°C for 7 days. Single fungal colonies with significant differences in morphology, color, and size were picked and transferred to new PDA plates. The purification process was repeated until homozygous single strains were obtained. The composition of potato dextrose agar (PDA) was: 200 g / L potato, 20 g / L glucose, and 20 g / L agar.

[0032] Initial screening culture: Using a circular punch with a diameter of 6.0 mm, holes were punched in the above homozygous single strains to obtain circular mycelial growths. These were placed in the center of a modified Aleksandrov culture plate and incubated statically at 28°C. The size of the potassium-releasing zone was observed.

[0033] The modified Aleksandrov medium formula is as follows: 5g glucose, 2g Na2HPO4, 0.5g MgSO4·7H2O, 0.1g CaCO3, 0.005g FeCl3, 2g potassium feldspar powder, 18g agar, 0.1g bromothymol blue, and deionized water to a final volume of 1L, adjusting the pH to 7.2±0.2; the potassium feldspar powder needs to be ground in advance and passed through a 200-mesh sieve.

[0034] Observe and select a strain with a distinct and large diameter yellow halo on a modified Aleksandrov culture plate, such as Figure 1 As shown, the strain was identified as having good potassium solubilization effect and was named SK-1.

[0035] (2) Molecular identification of Aspergillus tabingensis SK-1

[0036] Strains SK-1 were inoculated into PDA medium and cultured at 28°C for 7 days. Colony morphology observation showed that strain SK-1 initially developed loose, short, fluffy white mycelia on PDA medium, and black spores appeared after 3 days. Figure 2 As shown, the spore is a free single-celled spore, spherical with smooth edges, colorless and transparent. It is preliminarily determined that strain SK-1 is a mold.

[0037] Using EasyPure from Beijing TransGen Biotechnology Co., Ltd. ® The Plant Genomic DNA Kit was used to extract genomic DNA from strain SK-1. The transcribed spacer (ITS) sequence was amplified by PCR using primers ITS4 and ITS5. The ITS4 primer sequence was 5'-TCCTCCGCTTATTGATATGC-3' (SEQ ID NO. 2), and the ITS5 primer sequence was 5'-GGAAGTAAAAGTCGTAACAAGG-3' (SEQ ID NO. 3). The total PCR reaction volume was 25 µL, including 12.5 µL of Premix Taq, 1 µL each of the upper and lower primers, 8.5 µL of ddH2O, and 2 µL of DNA template. The PCR reaction program was as follows: (1) Pre-denaturation at 94 °C for 10 min; (2) Cycle 30 times according to the following program: denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, extension at 72 °C for 70 s; (3) Extension at 72 °C for 5 min. The amplified product was purified and recovered and sent to a sequencing company for sequencing.

[0038] The sequencing result of the ITS sequence is SEQ ID NO.1, as shown below:

[0039] .

[0040] The ITS sequences obtained from sequencing were BLAST-aligned using NCBI. The results showed that the top 7 sequences with the highest homology to strain SK-1 were all from *Aspergillus tubingensis*, with an identity of over 99%. Among them, the ITS sequence (OQ678245.1) showed 100% identity with *Aspergillus tubingensis* TPDA-1, indicating that strain SK-1 belongs to *Aspergillus tubingensis*. Therefore, the isolated *Aspergillus tubingensis* SK-1 was deposited on August 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), classified as *Aspergillus tubingensis*, with accession number CGMCC No. 42163, and deposited in Beijing, China.

[0041] Example 2

[0042] The application of a potassium-solubilizing Aspergillus tabineus strain SK-1 in woody plant cultivation, with the following specific steps:

[0043] (1) Potassium-solubilizing Aspergillus tabineus SK-1 was inoculated into potato glucose (PDB) culture medium. The PDB culture medium consisted of 200 g / L potato and 20 g / L glucose. The mixture was shaken and cultured at 28°C and 180 rpm for 6 days to obtain Aspergillus tabineus SK-1 fermentation broth.

[0044] (2) Dilute the Aspergillus tabineus SK-1 fermentation broth obtained in step (1) with water 10-100 times, with a viable count > 1×10⁻⁶. 8 CFU / mL, obtained aspergillus tabine SK-1 liquid inoculum;

[0045] (3) During the transplanting period of tissue culture seedlings, the liquid fungicide Aspergillus tabini SK-1 obtained in step (2) is applied to the roots of woody plants at a dosage of 10 mL / plant.

[0046] Example 3

[0047] The application of a potassium-solubilizing Aspergillus tabineus strain SK-1 in woody plant cultivation, with the following specific steps:

[0048] (1) Potassium-solubilizing Aspergillus tabineus SK-1 was inoculated into potato glucose (PDB) culture medium. The PDB culture medium consisted of 200 g / L potato and 20 g / L glucose. The mixture was shaken and cultured at 28℃ and 180 rpm for 6 days to obtain Aspergillus tabineus SK-1 fermentation broth.

[0049] (2) The Aspergillus tabineus SK-1 fermentation broth obtained in step (1) is mixed with corn straw powder and wheat bran powder in a ratio of 1mL:2g:6g, dried at 50℃ and then pulverized. The viable count is >1×10 8 CFU / g, obtained aspergillus tabine SK-1 solid inoculum agent;

[0050] (3) During the transplanting period of tissue culture seedlings, the Aspergillus tabine SK-1 solid inoculant obtained in step (2) is mixed with the soil at a dosage of 10g / plant and then applied in the hole.

[0051] Example 4

[0052] The application of a potassium-solubilizing Aspergillus tabineus strain SK-1 in woody plant cultivation, with the following specific steps:

[0053] (1) Potassium-solubilizing Aspergillus tabineus SK-1 was inoculated into potato glucose (PDB) culture medium. The PDB culture medium consisted of 200 g / L potato and 20 g / L glucose. The mixture was shaken and cultured at 28℃ and 180 rpm for 6 days to obtain Aspergillus tabineus SK-1 fermentation broth.

[0054] (2) The Aspergillus tabineus SK-1 fermentation broth obtained in step (1) is mixed with corn straw powder and wheat bran powder in a ratio of 1mL:2g:6g, dried at 50℃ and then pulverized. The viable count is >1×10 8 CFU / g, obtained aspergillus tabine SK-1 solid inoculum agent;

[0055] (3) During the topdressing period of tissue culture seedling growth, mix the Aspergillus tabineus SK-1 solid microbial agent obtained in step (2) with the soil at a dosage of 200g / plant and apply it in the hole.

[0056] Example 5

[0057] The application of a potassium-solubilizing Aspergillus tabineus strain SK-1 in woody plant cultivation, with the following specific steps:

[0058] (1) Potassium-solubilizing Aspergillus tabineus SK-1 was inoculated into potato glucose (PDB) culture medium. The PDB culture medium consisted of 200 g / L potato and 20 g / L glucose. The mixture was shaken and cultured at 28°C and 180 rpm for 6 days to obtain Aspergillus tabineus SK-1 fermentation broth.

[0059] (2) Dilute the Aspergillus tabineus SK-1 fermentation broth obtained in step (1) with water 10-100 times, with a viable count > 1×10⁻⁶. 8 CFU / mL, obtained aspergillus tabine SK-1 liquid inoculum;

[0060] (3) During the topdressing period of tissue culture seedling growth, apply the Aspergillus tabinei SK-1 liquid inoculant obtained in step (2) to the roots of woody plants at a dosage of 200 mL / plant.

[0061] Experimental Example 1: Determination of the potassium-solubilizing ability of Aspergillus tabineum SK-1

[0062] A small amount of *Aspergillus tabinei* SK-1 was inoculated into Aleksandrov liquid medium and cultured at 28℃ and 180 rpm for 7 days to obtain the fermentation broth. The fermentation broth was centrifuged at 12000 rpm for 10 min, and the supernatant was collected. After sterilization through a 0.22 μm filter membrane, the concentration of available potassium was determined using a flame spectrophotometer. Aleksandrov liquid medium without *Aspergillus tabinei* SK-1 inoculation served as the control group.

[0063] The Aleksandrov liquid culture medium formula is as follows: 5.0g glucose, 0.5g MgSO4·7H2O, 0.1g CaCl2, 0.005g FeCl3, 1.0g potassium feldspar powder, deionized water to a final volume of 1.0L, pH adjusted to 7.0-7.5, and sterilized at 121℃ for 20min.

[0064] After 7 days of culture in Aleksandrov liquid medium, the soluble potassium content of Aspergillus tabinei SK-1 reached 186.8 mg / L, indicating that Aspergillus tabinei SK-1 has excellent dissolution efficiency for potassium feldspar (K2O·Al2O3·6SiO2) and strong potassium solubilization ability.

[0065] Experiment Example 2: Effects of Aspergillus tabineus SK-1 liquid inoculant on the growth of potted Catalpa bungei seedlings

[0066] Healthy catalpa seedlings were selected and transplanted into sterilized vermiculite-based plastic pots. Ten pots were placed in each group, with catalpa trees treated with Aspergillus tabineus SK-1 liquid inoculant as the treatment group and those not treated as the control group. Each treatment group received 10 mL of Aspergillus tabineus SK-1 liquid inoculant (viable count > 1 × 10⁻⁶) per tree. 8 The control group was drenched with an equal volume of sterilized Aspergillus tabineus SK-1 liquid inoculant (with zero viable bacteria). During the maintenance period, the substrate was watered with distilled water every 3 days to maintain a moisture content of 60%. After one month of cultivation under conditions of 2000-3000 lx light intensity, a photoperiod of 16h / 8h, and a cultivation temperature of 25±2℃, the growth of the Catalpa trees in each group was observed (see [link to Catalpa tree growth information]). Figure 3 The potassium content in the leaves of the treated and control groups of *Catalpa bungei* trees was determined. The specific steps for determining the potassium content in the leaves are as follows: Leaves from the top of each *Catalpa bungei* plant were taken, dried at 80℃ to constant weight, pulverized, digested with HNO3∶HClO4=3∶1 (v / v), and after being brought to a final volume, the potassium content was determined using a flame photometer. The potassium content of the leaves in each group is expressed as the average potassium content of the leaves from 10 *Catalpa bungei* plants in that group.

[0067] Table 1. Determination of potassium content in catalpa leaves in the treatment and control groups.

[0068]

[0069] from Figure 3 It can be seen that the catalpa trees in the treatment group treated with Aspergillus tabin SK-1 liquid inoculant grew significantly better than those in the control group not treated with Aspergillus tabin SK-1 liquid inoculant. The catalpa trees in the treatment group grew vigorously, were taller, and had larger, brighter, darker green leaves. In contrast, the catalpa trees in the control group grew weaker, were shorter, had lower color brightness, smaller leaves, and had withered edges.

[0070] Table 1 shows that the potassium content in the leaves of the treated *Catalpa bungei* group was 119.2 ± 8.2 mg / kg, while the potassium content in the leaves of the control group was 63.8 ± 10.3 mg / kg. Compared with the control group, the potassium absorption of the treated *Catalpa bungei* group increased by 86.8%. This indicates that the application of *Aspergillus tabineum* SK-1 liquid inoculant can significantly promote the growth of *Catalpa bungei*, and that *Aspergillus tabineum* SK-1 can significantly improve the absorption efficiency of potassium by *Catalpa bungei*, thus promoting its growth.

[0071] Experiment Example 3: Effects of Aspergillus tabineum SK-1 solid inoculant on the growth of potted poplar seedlings under potassium deficiency stress

[0072] To further verify the growth-promoting effect of Aspergillus tabingii SK-1 on woody plants and its application effect in cultivation, poplar was selected as a woody plant for the experiment.

[0073] Healthy tissue-cultured poplar seedlings were selected and transplanted into sandy loam soil (garden soil and river sand mixed at a mass ratio of 1:1) that had been sun-dried and sterilized before being washed. The experiment was divided into a treatment group and a control group. In the treatment group, each poplar tree was treated with 10g of Aspergillus tabine SK-1 solid inoculant (viable count > 1 × 10⁻⁶) at the base of its roots. 8 The mixture of corn stalk powder (CFU / mL) and soil was applied in planting holes. The control group received an equal mixture of corn stalk powder and wheat bran powder (mass ratio 1:3). After application, the soil was thoroughly watered. Each group had 20 replicates. Then, every 3 days, the plants were uniformly irrigated with modified Hoagland nutrient solution (KNO3 content halved) to simulate potassium deficiency stress. The culture period was 8 weeks, during which the environmental conditions were maintained at a photoperiod of 16h / 8h and a temperature of 25±2℃. After the experiment, if... Figure 4 As shown, observe the growth status of poplar trees in the treatment group and the control group.

[0074] The modified Hoagland nutrient solution contains the following ingredients:

[0075] Macroelements: Ca(NO3)2·4H2O 945mg / L, KNO3 253mg / L (506mg / L in the classic formula, halved), NH4NO3 80mg / L, KH2PO4 136mg / L, MgSO4·7H2O 493mg / L;

[0076] Iron salt solution: 2.5 mL / L (containing 5.56 g / L FeSO4·7H2O + 27.46 g / L EDTA-Na, pH=5.5);

[0077] Trace element solution: 5 mL / L (containing KI 0.83 mg / L, H3BO3 6.2 mg / L, MnSO4 22.3 mg / L, ZnSO4 8.6 mg / L, CuSO4 0.025 mg / L, CoCl2 0.025 mg / L, Na2MoO4 0.25 mg / L).

[0078] Depend on Figure 4 It was found that the poplar trees treated with Aspergillus tabineum SK-1 solid inoculant showed significant differences under potassium deficiency stress compared to the control group without the inoculant. The treated poplar trees were generally upright and grew well, with greener leaves and only a few leaves showing yellowing at the edges. They exhibited better biomass accumulation and stronger adaptability. In contrast, the control group poplar trees were wilted, with severely yellowed leaves, numerous scorched patches on the leaf tips and edges, severely dried and curled leaves, and low biomass accumulation. The treated poplar trees showed less leaf loss and higher biomass accumulation, demonstrating stronger resistance to potassium deficiency stress. The applied Aspergillus tabineum SK-1 solid inoculant was able to activate insoluble potassium in the soil and enhance the root system's ability to absorb potassium, while the control group, lacking the inoculant, suffered from hindered potassium absorption and restricted growth.

[0079] Therefore, Aspergillus tabbina SK-1 solid inoculant can effectively promote the growth of poplar trees under potassium deficiency stress. It is suitable for nursery seedling cultivation, tree fertilization, forest soil improvement and ecological restoration projects, and has significant ecological and economic benefits.

Claims

1. A potassium-solubilizing Aspergillus tabineus SK-1, characterized in that: The Aspergillus tabineus SK-1 was deposited at the China General Microbiological Culture Collection Center, Beijing, China, and was classified as Aspergillus tabineus. Aspergillus tubingensis The accession number is CGMCC No.42163.

2. The application of the potassium-solubilizing Aspergillus tabineus SK-1 as described in claim 1 in the cultivation of woody plants, characterized in that: The application includes the following steps: (1) The Aspergillus tabineum SK-1 was fermented and cultured to obtain Aspergillus tabineum SK-1 fermentation broth; (2) Prepare the Aspergillus tabineus SK-1 fermentation broth obtained in step (1) into Aspergillus tabineus SK-1 inoculant and apply it to the planting soil of woody plants.

3. The application of the potassium-solubilizing Aspergillus tabineus SK-1 according to claim 2 in the cultivation of woody plants, characterized in that: In step (1), the method for preparing the fermentation broth of Aspergillus tabineus SK-1 is as follows: the Aspergillus tabineus SK-1 is inoculated into potato glucose culture medium and cultured under shaking conditions of 28℃ and 180rpm for 5-10 days to obtain the fermentation broth of Aspergillus tabineus SK-1.

4. The application of potassium-solubilizing Aspergillus tabineus SK-1 according to claim 3 in the cultivation of woody plants, characterized in that: In step (2), the Aspergillus tabineus SK-1 agent includes either Aspergillus tabineus SK-1 liquid agent or Aspergillus tabineus SK-1 solid agent.

5. The application of the potassium-solubilizing Aspergillus tabineus SK-1 according to claim 4 in the cultivation of woody plants, characterized in that: The preparation method of the Aspergillus tabineus SK-1 liquid inoculum is as follows: the Aspergillus tabineus SK-1 fermentation broth obtained in step (1) is diluted with water 10-100 times, and the viable count is >1×10 8 CFU / mL.

6. The application of the potassium-solubilizing Aspergillus tabineus SK-1 according to claim 4 in the cultivation of woody plants, characterized in that: The preparation method of the Aspergillus tabineus SK-1 solid inoculum is as follows: the Aspergillus tabineus SK-1 fermentation broth obtained in step (1) is mixed with corn straw powder and wheat bran powder in a ratio of 1mL:2g:6g, dried at 50℃ and then pulverized, with a viable count >1×10 8 CFU / g.

7. The application of potassium-solubilizing Aspergillus tabineus SK-1 according to claim 4 in the cultivation of woody plants, characterized in that: In step (2), the application method of Aspergillus tabineum SK-1 inoculant is as follows: during the transplanting period of tissue culture seedlings, apply Aspergillus tabineum SK-1 liquid inoculant to the roots of woody plants at a dosage of 10 mL / plant or apply Aspergillus tabineum SK-1 solid inoculant mixed with soil at a dosage of 10 g / plant in holes; during the topdressing period of tissue culture seedling growth, apply Aspergillus tabineum SK-1 liquid inoculant to the roots of woody plants at a dosage of 200 mL / plant or apply Aspergillus tabineum SK-1 solid inoculant mixed with soil at a dosage of 200 g / plant in holes.

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