Formula tea and tea drink for promoting blood sugar reduction

Abstract

The invention provides formula tea and tea drink capable of promoting blood sugar reduction, and the formula tea and tea drink are prepared from the following components in parts by mass: 0.6-1 part of folium mori, 1.8-2.5 parts of lithocarpus litseifolius, 1.8-2.2 parts of white tea and 0.1-0.18 part of folium mori extract. After being brewed, the tea drink contains a plurality of components such as caffeine, trilobatin, agritol 4 '-O-(5'-O-acetyl)-beta-d-glucopyranoside, phlorizin, D-glucose, 4-O-beta-D-galactopyranosyl, sedoheptulose and the like. The research shows that the blood sugar reducing tea drink has the function of maintaining the blood sugar health level on mice according to the national health and health committee 2023 edition of Health Food Function Test and Evaluation Method, and can effectively reduce blood sugar.

Description

Technical Field

[0001] This invention relates to the field of functional tea technology, specifically to a formula tea and tea beverage that promotes blood sugar reduction. Background Technology

[0002] Blood sugar-lowering health teas are functional beverages made primarily from medicinal and edible plants, using scientific formulations and modern processing techniques. Their core objective is to regulate blood sugar metabolism, improve insulin sensitivity, and serve as an adjunct to health management for patients with prediabetes and type II diabetes. The blood sugar-lowering effects of these health teas are achieved mainly through the following pathways: Inhibiting glucose absorption: Alpha-glucosidase inhibitors (such as 1-deoxynojirimycin (DNJ) from mulberry leaves) slow down carbohydrate breakdown, reducing postprandial blood sugar; dietary fiber (such as inulin and oat β-glucan) reduces intestinal glucose absorption; promoting glucose metabolism: Activating the AMPK (adenosine monophosphate-activated protein kinase) and PPAR-γ (peroxisome proliferator-activated receptor γ) signaling pathways, enhancing glucose uptake by muscle and fat cells.

[0003] Lowering blood sugar through functional teas is a suitable daily practice; therefore, this application proposes a functional tea and functional tea beverage that effectively lowers blood sugar. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a formula tea and tea beverage that promotes blood sugar reduction.

[0005] As one aspect of the present invention, the present invention provides a functional tea that effectively lowers blood sugar, which is composed of mulberry leaf composition, Litsea cubeba leaf and white tea, in parts by weight of 0.6-1.3 parts, 1.8-2.5 parts, 1.8-2.2 parts and 0.1-0.18 parts respectively.

[0006] Preferably, the mulberry leaf composition comprises mulberry leaves and / or mulberry leaf extract in a mass ratio of (0.6-1):(0.1-0.18).

[0007] Preferably, by weight, the amounts of mulberry leaf, Litsea cubeba, white tea, and mulberry leaf extract are 0.8 parts, 2.3 parts, 2 parts, and 0.15 parts, respectively.

[0008] In another aspect of the present invention, the formula tea is brewed using a water extraction method, with the amount of water being 800 parts by weight.

[0009] Preferably, by weight percentage, it includes 7%-8% caffeine, 30%-35% trifolin, 7%-8% sedulyl 4'-O-(5'-O-acetyl)-β-d-glucopyranoside, 6%-8% phlorizin, 5%-6% D-glucose, 4-O-β-D-galactopyranoside, 5%-6% sedoheptulose, 1%-2% persicogenin-3'-glucopyranoside, 1%-2% 10-methoxy-20-o-acetylcamptothecin, 1%-2% tocopherol phenyl isopropanol, and 1%-2% cis-1-(2-furanyl)-4-(2-thienyl)-1-buten-3-yne.

[0010] Preferably, by weight percentage, it comprises 7.73% caffeine, 34.70% trifolin, 7.08% 4'-O-(5'-O-acetyl)-β-d-glucopyranoside, 6.96% phlorizin, 5.63% D-glucose, 4-O-β-D-galactopyranosyl, 5.07% sedoheptulose, 1.86% persicogenin-3'-glucopyranoside, 1.25% 10-methoxy-20-o-acetylcamptothecin, 1.15% tocopherol phenyl isopropanol ((+)DELTA-tocopherol), and 1.12% cis-1-(2-furanyl)-4-(2-thienyl)-1-buten-3-yne.

[0011] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides a functional tea beverage based on white tea, which, after brewing, contains caffeine, trifolin, 4'-O-(5'-O-acetyl)-β-d-glucopyranoside, phlorizin, D-glucose, 4-O-β-D-galactopyranosyl, sedoheptulose, and other components. According to the 2023 edition of the "Methods for Functional Testing and Evaluation of Health Foods" issued by the National Health Commission of China, this study shows that the hypoglycemic tea beverage can maintain healthy blood sugar levels in mice and can effectively lower blood sugar. Attached Figure Description

[0012] Figure 1 This is a graph showing the top 10 detected components in Example 1 of the present invention; Figure 2 This is a distribution diagram of the number of components classified in Embodiment 1 of the present invention; Figure 3 This is a distribution diagram of the component categories and contents in Example 1 of the present invention; Figure 4 This describes the extraction process of mulberry leaf extract in Example 1 of the present invention. Detailed Implementation

[0013] To provide a clearer understanding of the technical features, objectives, and effects of the present invention, specific embodiments of the present invention are now described.

[0014] Example 1 According to Table 1, mulberry leaves, Litsea cubeba leaves, white tea, and mulberry leaf extract were weighed separately by weight to form a formula tea that promotes blood sugar reduction. Table 1 .

[0015] Mulberry leaf extract was prepared by water extraction or alcohol extraction, ensuring that the content of deoxynojirimycin C6H13NO4 in the mulberry leaf extract was greater than or equal to 5%. This example uses the alcohol extraction method; see details below. Figure 4 The content of deoxynojirimycin C6H13NO4 in the obtained mulberry leaf extract was between 0.76 and 0.79 (see M0060 in Table 3).

[0016] The herbal tea is brewed using the water extraction method with 800ml of water. The specific brewing method is as follows: After selecting the raw materials, white tea was roasted at 60℃ for 2 hours, Litsea cubeba for 1 hour, and mulberry leaves for 1 hour. The white tea and Litsea cubeba were then pulverized, mixed, and stirred. 800ml of water was added and boiled to 100℃. Mulberry leaves were then added, and the mixture was allowed to stand at room temperature before filtering. Mulberry leaf extract was then added, stirred, and filtered again to obtain the tea beverage. The components of the tea beverage were analyzed. The experimental procedures are as follows: 1) Preprocessing Take out the tea sample, mix it evenly, and put 100 μL of the sample into a 1.5 mL centrifuge tube; Add 900 μL of 70% methanol-water mixture (containing mixed internal standard, 2 μg / mL) and vortex for 1 min; Ultrasonic extraction in an ice-water bath for 60 min; Centrifuge for 10 min (12000 rpm, 4℃); Take 200 μL of the supernatant and transfer it into an LC-MS vial with an inner liner for analysis. Test 3 samples.

[0017] 2) Liquid Chromatography-Mass Spectrometry Conditions Column: ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 μm); Column temperature: 45℃; Mobile phase (Table 2): A-water (containing 0.1% formic acid), B-acetonitrile; Flow rate: 0.35 mL / min; Injection volume: 2 μL.

[0018] PDA scanning range: 210-400 nm; ion source: HESI; sample mass spectrometry signal acquisition adopted positive and negative ion scanning modes respectively.

[0019] Data acquisition mode: DDA. Scan method: Full MS / dd-MS2 (TOP 8). Table 2 .

[0020] Table 3 shows the test results, including information on components and retention times. , , , , , , , , , , , , , , , , , , .

[0021] Example 4: Study on the effect of hypoglycemic tea on blood glucose in mice The blood sugar-lowering tea, obtained in Example 1, is a dark red liquid. Based on an average adult weight of 75 kg, the formula in Example 1 contains 0.8 g of mulberry leaves, 2.3 g of Litsea cubeba, 2 g of white tea, and 0.15 g of mulberry leaf extract, totaling 5.25 g. The recommended human dosage is calculated as 5.25 g / 75 kg = 0.075 g / kg. Using 0.075 g / kg as the normal dosage, further multiplying by the corresponding factors to convert to mouse dosages, we arrive at the high-dose group (0.75 g / kg), medium-dose group (0.375 g / kg), and low-dose group (0.1875 g / kg).

[0022] A total of 70 SPF-grade male Kunming mice provided by Beijing Speywood Biotechnology Co., Ltd. were selected as experimental animals, with a body weight of 24.0 - 28.0 g. The animal quality certificate number is 11324251101147778, and the animal production license number is SCXK(Beijing)2024-0001. The mice were allowed to freely eat and drink water, and were raised in an environment with a temperature of 20°C - 24°C, a relative humidity of 50% - 60%, and a 12 h / 12 h light-dark cycle. The feed and bedding were both provided by Beijing Speywood Biotechnology Co., Ltd. The test substance was set at 3 dose groups, a high-dose group (0.75 g / kg), a medium-dose group (0.375 g / kg), and a low-dose group (0.1875 g / kg), which were equivalent to 10.5 and 2.5 times the recommended human dosage respectively. Another model control group was set, and equal volume of distilled water was given. The gavage volume was 0.2 mL / 10 g for all groups.

[0023] Experimental methods Effect on blood glucose of normal mice: 20 healthy mice were selected for the experiment. They were grouped according to their blood glucose levels after a 5-hour fasting period, and randomly divided into 1 normal control group and 1 dose group, with 10 animals in each group, and fed separately in cages. The control group was given an equal volume of solvent distilled water, and the dose group was orally administered the high-dose concentration of the test sample (0.75 g / kg). The gavage volume was 0.2 mL / 10 g. This was done continuously for 30 days. The body weights of the animals were measured on days 0, 15, and 30 of the experiment. The weight gain of the animals was calculated on day 30 of the experiment. The fasting blood glucose (after a 5-hour fasting period) was measured on day 30. The body weights, weight gains, and blood glucose values of the two groups of animals were compared.

[0024] Effect on blood glucose of hyperglycemic model mice with pancreatic islet injury: After 50 mice were acclimated for 3 days, 15 animals were randomly selected and fasted for 5 hours, and their fasting blood glucose was measured as the baseline blood glucose value of this batch of animals. Subsequently, after the animals were fasted for 24 hours (allowed to drink water freely), they were intraperitoneally injected with alloxan (130 mg / kg) once to establish the model. Five days later, they were fasted for 5 hours and their blood glucose was measured.

[0025] Forty healthy mice with a successful hyperglycemia model (blood glucose levels between 10-25 mmol / L), glossy coats, and good mental state were selected. They were randomly divided into a model control group and three dosage groups based on their blood glucose levels after a 5-hour fast. Each group consisted of 10 animals, housed separately. Different concentrations of the test sample were administered to each dosage group, while the model control group received an equal volume of distilled water, once daily for 30 consecutive days. Animal weight was measured on days 0, 15, and 30, and weight gain was calculated on day 30. Fasting blood glucose levels were measured (after a 5-hour fast). Each dosage group was then given the corresponding concentration of the test sample again, while the model control group received an equal volume of solvent. Fifteen minutes later, each group was orally administered glucose (2.0 g / kg), and blood glucose levels were measured at 0.5 and 2.0 hours after glucose administration. The fasting blood glucose levels and the percentage decrease in blood glucose were compared among the groups of animals. Changes in blood glucose levels and the area under the blood glucose curve were observed at various time points (0, 0.5, and 2.0 h) after glucose administration in the model control group and the dosage group. The formulas for calculating the rate of decrease in blood glucose and the area under the blood glucose curve were: Blood glucose decrease rate (%) = (Blood glucose level before administration of the test substance - Blood glucose level at the end of the experiment) / Blood glucose level before administration of the test substance × 100% and Area under the blood glucose curve = (0 h blood glucose + 0.5 h blood glucose) × 0.5 / 2 + (2 h blood glucose + 0.5 h blood glucose) × 1.5 / 2.

[0026] Statistical analysis was performed using SPSS 22.0 software. In the experiment on the effects of the blood glucose-lowering beverage on fasting blood glucose and body weight in normal mice, an independent samples t-test was used to compare the normal control group and the dosage group. In the experiment on the effects of the blood glucose-lowering beverage on fasting blood glucose and body weight in hyperglycemic model mice, one-way ANOVA was used to compare the three dosage groups with the model control group. If statistically significant differences were found, Dunnett-t tests were used for pairwise comparisons between multiple dosage groups and the control group. Since the variances of the blood glucose reduction rate were unequal, a rank-sum test was used for inter-group comparisons. The significance level was set at α=0.05 (two-tailed).

[0027] Animal growth: During the experiment, normal mice had neat and shiny fur, and no abnormalities were observed in their mental state, behavior, or feces. There was no statistically significant difference in weight gain between the dosage group and the normal control group after 30 days (P>0.05), as shown in Table 4 (effects of the hypoglycemic beverage on blood glucose and weight in normal mice, n=10). The hyperglycemic model mice exhibited significant polyuria, and the cages emitted an odor of rotten apples. The hyperglycemic model mice showed significantly greater weight gain at 30 days compared to normal mice (P<0.01). The high, medium, and low dosage groups showed significantly less weight gain at 30 days compared to the model control group (P<0.01), as shown in Table 5. Table 4 .

[0028] Effects of hypoglycemic beverages on fasting blood glucose in normal mice: Before and after the experiment, there were no statistically significant differences in fasting blood glucose levels between the normal control group and the dosage group (all P>0.05), as shown in Table 3. Table 5 .

[0029] Effects of hypoglycemic beverages on fasting blood glucose in hyperglycemic model mice: Before modeling, the basal blood glucose level of this batch of mice was (8.5±0.8) mmol / L. The fasting blood glucose level of the hyperglycemic model mice was significantly higher than that of this batch of mice, indicating successful modeling. At the end of the experiment, the fasting blood glucose levels of all dose groups of the hyperglycemic model mice were lower than those of the model control group, and the rate of blood glucose reduction in each dose group was higher than that in the model control group. The differences in the rate of blood glucose reduction in the high, medium, and low dose groups compared with the model control group were statistically significant (P<0.01, P<0.05), as shown in Table 6.

[0030] Effects of hypoglycemic beverages on glucose tolerance in hyperglycemic model mice: Blood glucose levels and area under the glucose curve (AUC) at all time points after glucose administration in all dose groups of the hyperglycemic model mice were lower than those in the model control group. Specifically, the blood glucose levels at 0.5 h after glucose administration in the high and medium dose groups were significantly different from those in the model control group (P<0.01); the blood glucose levels at 2 h after glucose administration in the high, medium, and low dose groups were significantly different from those in the model control group (all P<0.01); and the AUC of the blood glucose curve in the high, medium, and low dose groups were significantly different from those in the model control group (all P<0.01). (See Table 7). Table 6. Effects of blood sugar-lowering beverages on fasting blood glucose in hyperglycemic model mice (n=10) .

[0031] Table 7 .

[0032] According to the provisions of the National Health Commission's 2023 edition of "Methods for Functional Testing and Evaluation of Health Foods" regarding methods for testing methods that help maintain healthy blood glucose levels, this study shows that the hypoglycemic tea has the function of maintaining healthy blood glucose levels in mice.

[0033] This invention adjusts the white tea formula to stimulate the release of effective components, especially the synergistic effects of 1-deoxynojirimycin (DNJ), mulberry leaf polysaccharides (such as 4'-O-(5'-O-acetyl)-β-d-glucopyranoside, D-glucose, 4-O-β-D-galactopyranoside, sedoheptulose, etc.), quercetin, and phlorizin, thus systematically enhancing the hypoglycemic function of white tea as a health tea.

[0034] 1-Deoxynojirimycin (DNJ) exhibits a non-competitive inhibitory effect on α-sucrase. DNJ promotes glucose uptake and degradation in the liver by up- / down-regulating the expression of rate-limiting enzymes in glucose metabolism, thereby reducing glucose production and regulating blood glucose levels. Mulberry leaf polysaccharides can reduce the levels of inflammatory mediators and free fatty acids in diabetic rats induced by a high-fat diet and a high-sugar, high-fat diet combined with streptozocin (STZ). They also reduce pancreatic islet cell apoptosis by increasing B-cell lymphoma-2 (Bcl-2) / Bcl-2-related X protein (Bax), and improve the insulin secretion capacity of pancreatic β-cells by restoring PDX-1 nuclear localization and protein expression in diabetic rats. Quercetin exhibits α-glucosidase inhibitory activity in vitro. It increases glucose uptake by muscle cells by activating the CaMKKβ / AMPK pathway and insulin signaling pathway. Simultaneously, it enhances glucokinase (GCK) expression levels in diabetic rats, strengthens Akt and GSK-3 phosphorylation, regulates GSK-3β activity in the liver, and alleviates inhibition of glycogen synthase. Furthermore, it inhibits α-glucosidase catalytic activity by interacting with the active sites of the α-glucosidase receptor (Asp 69, Asp 215, Arg 315, Arg 442, Gln 353, Glu 411, and Gln 279) to form ten hydrogen bonds. This inhibition enhances insulin sensitivity and insulin signaling in db / db mice, thereby improving fasting hyperglycemia. Phlorizin from Litsea cubeba is a specific and competitive inhibitor of SGLT1 and SGLT2. It can restore normal blood glucose levels and normalize insulin sensitivity by downregulating glucose absorption in the intestines and kidneys, thereby improving the occurrence of hyperglycemia, hypertension, and diabetic nephropathy complications in diabetic rats. Trifolin exhibits non-competitive inhibitory effects on α-glucosidase and α-amylase. It has a strong inhibitory effect on α-glucosidase and a moderate inhibitory effect on α-amylase. Trifolin inhibits α-glucosidase while avoiding the side effects caused by excessive inhibition of pancreatic amylase.

[0035] Based on the above analysis and the hypoglycemic effect of this experiment, this invention demonstrates that 1-deoxynojirimycin (DNJ), mulberry leaf polysaccharides, and other ingredients provide significant synergistic effects against α-sucrase and α-glucosidase.

[0036] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A formula tea that promotes blood sugar reduction, characterized in that: It is composed of mulberry leaf composition, Litsea cubeba leaf and white tea, in parts by weight of 0.6-1.3 parts, 1.8-2.5 parts, 1.8-2.2 parts and 0.1-0.18 parts respectively.

2. The formula tea for promoting blood sugar reduction according to claim 1, characterized in that: The mulberry leaf composition comprises mulberry leaves and / or mulberry leaf extract in a mass ratio of (0.6-1):(0.1-0.18).

3. The formula tea for promoting blood sugar reduction according to claim 2, characterized in that: By weight, the amounts of mulberry leaf, Litsea cubeba, white tea, and mulberry leaf extract were 0.8 parts, 2.3 parts, 2 parts, and 0.15 parts, respectively.

4. The formula tea for promoting blood sugar reduction according to claim 2, characterized in that: The mulberry leaves, the mulberry leaf extract, and the white tea are derived from the corresponding raw materials: tea leaves or tea powder.

5. The formula tea for promoting blood sugar reduction according to claim 2, characterized in that: The content of deoxynojirimycin C6H13NO4 in the mulberry leaf extract is greater than or equal to 5%.

6. The formula tea for promoting blood sugar reduction according to claim 2, characterized in that: The mulberry leaf extract is obtained by water extraction or alcohol extraction of mulberry leaves.

7. A tea beverage that promotes blood sugar reduction, characterized in that: The tea formula described in claim 1 is brewed using a water extraction method, with 800 parts by weight of water.

8. The tea beverage for promoting blood sugar reduction according to claim 7, characterized in that: By weight percentage, it includes 7%-8% caffeine, 30%-35% trifolin, 7%-8% sedulyl 4'-O-(5'-O-acetyl)-β-d-glucopyranoside, 6%-8% phlorizin, 5%-6% D-glucose, 4-O-β-D-galactopyranosyl, 5%-6% sedoheptulose, 1%-2% persicogenin-3'-glucopyranoside, 1%-2% 10-methoxy-20-o-acetylcamptothecin, 1%-2% tocopherol phenyl isopropanol, and 1%-2% cis-1-(2-furanyl)-4-(2-thienyl)-1-buten-3-yne.

9. The tea beverage for promoting blood sugar reduction according to claim 7, characterized in that: By weight percentage, it includes caffeine 7.73%, trifolin 34.70%, sedulyl 4'-O-(5'-O-acetyl)-β-d-glucopyranoside 7.08%, phlorizin 6.96%, D-glucose, 4-O-β-D-galactopyranoside 5.63%, sedulose heptanose 5.07%, persicogenin-3'-glucopyranoside 1.86%, 10-methoxy-20-o-acetylcamptothecin 1.25%, tocopherol phenyl isopropanol 1.15%, and cis-1-(2-furanyl)-4-(2-thienyl)-1-buten-3-yne 1.12%.

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